CN104165876A - Method for rapidly fluorescently detecting hydrogen peroxide on basis of etched nano silver triangle - Google Patents
Method for rapidly fluorescently detecting hydrogen peroxide on basis of etched nano silver triangle Download PDFInfo
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- CN104165876A CN104165876A CN201410418331.7A CN201410418331A CN104165876A CN 104165876 A CN104165876 A CN 104165876A CN 201410418331 A CN201410418331 A CN 201410418331A CN 104165876 A CN104165876 A CN 104165876A
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Abstract
The invention discloses a method for rapidly fluorescently detecting hydrogen peroxide on the basis of an etched nano silver triangle. The method comprises the following steps: mixing a sample which probably containing hydrogen peroxide with a nano silver triangle-bovine serum albumin-fluorescent dye composite in a selected liquid-phase environment to be reacted, and detecting the content of hydrogen peroxide in the sample by detecting the fluorescence intensity of a mixed reaction system, wherein the selected liquid-phase environment is a liquid-phase reaction environment which is suitable for the hydrogen peroxide to etch the nano silver triangle. The method has the advantages of high interference resistance, simplicity and convenience in operation, rapidness, sensitivity, low detection limit, low cost and the like, is suitable for rapidly and high-sensitively detecting a complicated matrix and does not need the complicated pretreatment.
Description
Technical field
The present invention relates to a kind of detection method of hydrogen peroxide, particularly a kind of method of the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle.
Background technology
Hydrogen peroxide (Hydrogen peroxide) chemical formula is H
2o
2, its aqueous solution is commonly called as hydrogen peroxide.Outward appearance is colourless transparent liquid, is a kind of strong oxidizer, and its aqueous solution is applicable to wound disinfection and environment, food sterilization.Hydrogen peroxide is not only the accessory substance of some high-selectivity oxidation enzyme reactions, and be a kind of important intermediate in food, pharmacy, clinical, environment and technical analysis, the detection method of research low concentration hydrogen peroxide is significant in the detection of food, medicine.Therefore the method that, detects easily and fast, exactly content of hydrogen peroxide seems particularly important.
The detection method of many hydrogen peroxide that at present had bibliographical information both at home and abroad, as spectroscopic methodology, chemoluminescence method and electrochemical process etc., but the shortcoming such as ubiquity sensitivity is not high, stability is not enough or sample substrate interference is strong, thereby limited their practical application.
Summary of the invention
Fundamental purpose of the present invention is to propose a kind of method of the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle, and it can be realized hydrogen peroxide is carried out to quick, sensitive detection, with low cost, easy operating, thus overcome deficiency of the prior art.
For achieving the above object, the present invention has adopted following technical scheme:
A kind of method of the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle, comprise: sample and Nano Silver triangle-bovine serum albumin-fluorescent dye compound hybrid reaction in selected liquid phase environment that may contain hydrogen peroxide, by detecting the fluorescence intensity of hybrid reaction system, the detection of realization to contained hydrogen peroxide in sample, described selected liquid phase environment is the liquid phase reactor environment that is suitable for hydrogen peroxide etching Nano Silver triangle.
Further, the preparation method of described Nano Silver triangle-bovine serum albumin-fluorescent dye compound can comprise: by Nano Silver triangle solution and bovine serum albumen solution hybrid reaction, add again fluorescent dye solution, fully after reaction, form described Nano Silver triangle-bovine serum albumin-fluorescent dye compound.
Wherein, the particle diameter of described Nano Silver triangle is preferably 30-50nm.
Described fluorescent dye can be selected from but be not limited to fluorescein isothiocynate or tetramethyl rhodamine-5-isothiocyanates.
As one of comparatively typical embodiment, the method for the described fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle specifically can comprise the steps:
(1) bovine serum albumen solution is added to Nano Silver triangle solution, after mixing, standing over night;
(2) fluorescent dye solution is added to the final institute of step (1) and obtains in mixed solution, after mixing, more than standing 4h;
(3) by the final institute of step (2) mixed solution that obtains with more than being not less than the centrifugal 20min of speed of 8000rpm, precipitate take citric acid three sodium solution that concentration is 0.015M resuspended after, standby;
(4) get the sample that may contain hydrogen peroxide and mix with the final resuspended solution that obtains of step (3), and at room temperature more than standing 20min, and detect the fluorescence intensity of the hybrid reaction system that obtains, the detection of realization to contained hydrogen peroxide in sample.
Further, the method for the described fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle comprises:
I, the hydrogen peroxide standard model of getting a series of variable concentrations and Nano Silver triangle-bovine serum albumin-fluorescent dye compound be hybrid reaction in selected liquid phase environment, and detection is by the fluorescence intensity of the final formed hybrid reaction system of each standard model, and, the corresponding relation Criterion curve of establishing criteria sample concentration and hybrid reaction system fluorescence intensity;
II, get and may contain the sample of hydrogen peroxide and Nano Silver triangle-bovine serum albumin-fluorescent dye compound hybrid reaction in selected liquid phase environment, and detect the fluorescence intensity of final formed hybrid reaction system, compare with typical curve again, thereby record the concentration of contained hydrogen peroxide in sample.
Further, the fluorescence intensity of described fluorescence intensity for detecting under the emission wavelength of described fluorescent dye.
Compared with prior art, the present invention at least tool has the following advantages: be somebody's turn to do the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle according to hydrogen peroxide etching Nano Silver triangle, and on Nano Silver triangle, be marked with fluorescent dye, obtain the mixed solution that fluorescence intensity is different, can be by measuring photocathode quantitative analysis results, detect easy to be quick, highly sensitive, with low cost.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, the accompanying drawing the following describes is only some embodiment that record in the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the schematic diagram that the present invention is based on the hydrogen peroxide fluorescence detection method of silver nanoparticle triangle etching;
Fig. 2 is concentration of hydrogen peroxide-Standardization curve for fluorescence intensity of setting up in the embodiment of the present invention 1;
Fig. 3 is Nano Silver triangle-bovine serum albumin(BSA)-fluorescein isothiocynate compound transmission electron microscope picture in the embodiment of the present invention 1;
Fig. 4 is that in the embodiment of the present invention 1, Nano Silver triangle-bovine serum albumin(BSA)-fluorescein isothiocynate compound and concentration are the transmission electron microscope picture after the superoxol of 100 μ M is hatched.
Embodiment
As previously mentioned, in view of deficiency of the prior art, the invention provides a kind of method of the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle, its by after silver nanoparticle triangle adsorption bovine serum albumin with fluorescein isothiocynate, the fluorescence molecule couplings such as tetramethyl rhodamine-5-isothiocyanates, obtain silver nanoparticle triangle-bovine serum albumin(BSA)-fluorescent dye compound, after hydrogen peroxide etching silver nanoparticle triangle, the fluorescence signal of nano-complex strengthens, and it is different to the etching degree of Nano Silver triangle according to the hydrogen peroxide of varying number, make the fluorescence intensity difference showing, thereby realize the content that quantitatively detects hydrogen peroxide, this detection method is easy and simple to handle, be not vulnerable to the interference of sample substrate, thereby do not need precipitation to remove the more loaded down with trivial details sample pre-treatments consuming time such as albumen yet, this is detection of complex matrix, comprise food, content such as low concentration hydrogen peroxide in the liquid beverages such as milk provides quick, high-sensitive detection method.
Method of the present invention comprises: sample and Nano Silver triangle-bovine serum albumin-fluorescent dye compound hybrid reaction in selected liquid phase environment that may contain hydrogen peroxide, by detecting the fluorescence intensity of hybrid reaction system, the detection of realization to contained hydrogen peroxide in sample, described selected liquid phase environment is the liquid phase reactor environment that is suitable for hydrogen peroxide etching Nano Silver triangle.
For example, as one of specific embodiment comparatively, the method can comprise the steps:
(1) bovine serum albumen solution is added in Nano Silver triangle solution, making the final concentration of bovine serum albumin is 0.5mg/mL, mixes standing over night.
(2) according to the mass ratio of fluorescent dye and bovine serum albumin, be 1:3 to 1:6, fluorescent dye solution is added to final the obtained mixed solution of step (1), mix standing 4h;
(3) by final the obtained mixed solution of step (2) at the centrifugal 20min of 8000rpm, it is resuspended that precipitation be take the citric acid three sodium solution that concentration is 0.015M, concentrated 10 times simultaneously.
(4) getting the test sample that may contain hydrogen peroxide mixes according to the volume ratio of 1:1 with the resuspended solution that obtains in step (3), and standing 20min at room temperature, detect the fluorescence intensity of hybrid reaction system, realize the detection to contained hydrogen peroxide in sample.
And one more specifically in case study on implementation, the method can comprise
(1) preparation of Nano Silver triangle.40mL water solution system, comprises 400 μ L, 0.01M liquor argenti nitratis ophthalmicus, 600 μ L, 0.1M citric acid three sodium solution, 96 μ L, 30wt%H
2o
2strong stirring 10min under room temperature.Then, inject fast 400 μ L, 0.1M sodium borohydride solution.
(2) get 40mL Nano Silver triangle solution.200 μ L bovine serum albumen solution (10mg/mL) are joined in Nano Silver triangle rubber liquid solution, mix standing over night.Again 300 μ L fluorescent dyes (1mg/mL) are joined in above-mentioned mixed solution, mix standing 4h.The centrifugal 20min of mixed solution 8000rpm, resuspended with 0.015M citric acid three sodium solution, concentrated 10 times simultaneously.
(3) draw variable concentrations (as: 0,0.025,5,30,50,100 μ M) hydrogen peroxide 100uL, join in the resuspended liquid described in (1), vibration mixes, standing 20min, the fluorescence intensity of detection hybrid reaction system.
Further, in abovementioned steps (1), described Nano Silver triangle rubber liquid solution volume can be 10～100mL.
The bovine serum albumen solution volume using in abovementioned steps (1) can also be 50～500 μ L, and concentration can also be 1～10mg/mL.
The fluorescent dye solution volume using in abovementioned steps (1) can also be 30～300 μ L, and concentration can also be 1～10mg/mL.
More specifically, the method in this embodiment can also be:
(1) prepare 10～100mL Nano Silver triangle rubber liquid solution.50～500 μ L bovine serum albumen solution (1～10mg/mL) are joined in Nano Silver triangle rubber liquid solution, mix standing over night.Again 30～300 μ L fluorescent materials (1～10mg/mL) are joined in above-mentioned mixed solution, mix standing 4h.The centrifugal 20min of mixed solution 8000rpm, resuspended with 0.015M citric acid three sodium solution, concentrated 10 times simultaneously.
(2) draw variable concentrations (as: 0,0.025,5,30,50,100 μ M) hydrogen peroxide 100uL, join in the resuspended liquid described in (1), vibration mixes, standing 20min, the fluorescence intensity of detection hybrid reaction system.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is described in detail, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, the every other embodiment that those of ordinary skills obtain under the prerequisite of not making creative work, belongs to the scope of protection of the invention.
The detection method principle of embodiment 1 this hydrogen peroxide is shown below:
2Ag+H
2O
2→2Ag
++2OH
-
The method is specific as follows:
(1) preparation of Nano Silver triangle-bovine serum albumin (BSA)-fluorescein isothiocynate (FITC) compound.Get 40mL Nano Silver triangle solution, 200 μ L bovine serum albumen solution (10mg/mL) are joined in Nano Silver triangle rubber liquid solution, mix standing over night.Again 300 μ L fluorescein isothiocynates (1mg/mL) are joined in above-mentioned mixed solution, mix standing 4h.The centrifugal 20min of mixed solution 8000rpm, resuspended with 0.015M citric acid three sodium solution, concentrated 10 times simultaneously.
(2) making of hydrogen peroxide typical curve.Draw variable concentrations (as: 0,0.025,5,30,50,100 μ M) superoxol 100uL, join in the resuspended liquid described in step (1), vibration mixes, standing 20min, detect the fluorescence intensity (excitation wavelength 494nm, emission wavelength 518nm) of hybrid reaction system.Take concentration of hydrogen peroxide as horizontal ordinate, and fluorescence intensity is ordinate, and as shown in Figure 2, detect the range of linearity is 0.025-100 μ M to the typical curve obtaining, and detection sensitivity can reach 8nM.
In addition, to refer to Fig. 3,4 be Nano Silver triangle-bovine serum albumin(BSA)-fluorescein isothiocynate compound, and being 100 μ M with concentration, superoxol is hatched forward and backward transmission electron microscope picture.
(3) detection of hydrogen peroxide in fresh milk sample.By 20 times of fresh milk dilutions, get solution 100 μ L, join in the resuspended liquid described in step (1), vibration mixes, standing 20min, the fluorescence intensity (excitation wavelength 494nm, emission wavelength 518nm) of detection hybrid reaction system.Reference standard curve can calculate the concentration of hydrogen peroxide in milk sample.
Embodiment 2 the method are specific as follows:
(1) preparation of Nano Silver triangle-bovine serum albumin-tetramethyl rhodamine-5-isothiocyanate complexes.Get 40mL Nano Silver triangle rubber liquid solution.200 μ L bovine serum albumen solution (10mg/mL) are joined in Nano Silver triangle rubber liquid solution, mix standing over night.Again 300 μ L tetramethyl rhodamine-5-isothiocyanates (1mg/mL) are joined in above-mentioned mixed solution, mix standing 4h.The centrifugal 20min of mixed solution 8000rpm, resuspended with 0.015M citric acid three sodium solution, concentrated 10 times simultaneously.
(2) draw variable concentrations (as: 0,0.025,5,30,50,100 μ M) superoxol 100uL, join in the resuspended liquid described in step (1), vibration mixes, standing 20min, detect the fluorescence intensity (excitation wavelength 546nm, emission wavelength 576nm) of hybrid reaction system.The testing result of hydrogen peroxide, also can draw the typical curve close with embodiment 1.
(3) detection of hydrogen peroxide in fresh milk sample: similar to method in example 1.
Be to be understood that; above embodiment is only for illustrating content of the present invention, and in addition, the present invention also has other embodiments; in every case those skilled in the art enlighten because inventing related technology, and adopt the technical scheme that is equal to replacement or equivalents formation all to drop in protection scope of the present invention.
Claims (7)
1. the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle, it is characterized in that comprising: sample and Nano Silver triangle-bovine serum albumin-fluorescent dye compound hybrid reaction in selected liquid phase environment that may contain hydrogen peroxide, by detecting the fluorescence intensity of hybrid reaction system, the detection of realization to contained hydrogen peroxide in sample, described selected liquid phase environment is the liquid phase reactor environment that is suitable for hydrogen peroxide etching Nano Silver triangle.
2. the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle according to claim 1, the preparation method who it is characterized in that described Nano Silver triangle-bovine serum albumin-fluorescent dye compound comprises: by Nano Silver triangle solution and bovine serum albumen solution hybrid reaction, add again fluorescent dye solution, fully after reaction, form described Nano Silver triangle-bovine serum albumin-fluorescent dye compound.
3. the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle according to claim 1, the particle diameter that it is characterized in that described Nano Silver triangle is 30-50 nm.
4. the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle according to claim 1 and 2, is characterized in that described fluorescent dye comprises fluorescein isothiocynate or tetramethyl rhodamine-5-isothiocyanates.
5. the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle according to claim 1, is characterized in that comprising the steps:
(1) bovine serum albumen solution is added to Nano Silver triangle solution, after mixing, standing over night;
(2) fluorescent dye solution is added to the final institute of step (1) and obtains in mixed solution, after mixing, more than standing 4 h;
(3) by the final institute of step (2) mixed solution that obtains with more than being not less than centrifugal 20 min of speed of 8000 rpm, precipitate take citric acid three sodium solution that concentration is 0.015 M resuspended after, standby;
(4) get the sample that may contain hydrogen peroxide and mix with the final resuspended solution that obtains of step (3), and at room temperature more than standing 20 min, and detect the fluorescence intensity of the hybrid reaction system that obtains, the detection of realization to contained hydrogen peroxide in sample.
6. according to the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle described in any one in claim 1-5, it is characterized in that comprising:
I, the hydrogen peroxide standard model of getting a series of variable concentrations and Nano Silver triangle-bovine serum albumin-fluorescent dye compound be hybrid reaction in selected liquid phase environment, and detection is by the fluorescence intensity of the final formed hybrid reaction system of each standard model, and, the corresponding relation Criterion curve of establishing criteria sample concentration and hybrid reaction system fluorescence intensity;
II, get and may contain the sample of hydrogen peroxide and Nano Silver triangle-bovine serum albumin-fluorescent dye compound hybrid reaction in selected liquid phase environment, and detect the fluorescence intensity of final formed hybrid reaction system, compare with typical curve again, thereby record the concentration of contained hydrogen peroxide in sample.
7. according to the method for the fluoroscopic examination hydrogen peroxide based on etching Nano Silver triangle described in any one in claim 1-5, it is characterized in that the fluorescence intensity of described fluorescence intensity for detecting under the emission wavelength of described fluorescent dye.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181953A (en) * | 2015-06-26 | 2015-12-23 | 江南大学 | Grape-cluster-like nanoparticles, immune probe, and preparation method and applications of immune probe |
| CN105731534A (en) * | 2016-02-03 | 2016-07-06 | 浙江理工大学 | Method for labeling titanium dioxide nanotubes with green fluorescence |
| CN106404739A (en) * | 2016-09-07 | 2017-02-15 | 江南大学 | Surface-enhanced Raman scattering substrate as well as preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590164A (en) * | 2012-02-03 | 2012-07-18 | 桂林理工大学 | Method for determining hydrogen peroxide content |
| CN103087328A (en) * | 2013-02-01 | 2013-05-08 | 北京理工大学 | Preparation method of chitosan 6-OH immobilized cyclodextrin derivative and application thereof in H2O2 detection fluorescent biosensor |
| KR20130107060A (en) * | 2012-03-21 | 2013-10-01 | 아주대학교산학협력단 | Changeable color two-photon fluorescent probes for hydrogen peroxide in mitochondria and biological imaging methode of hydrogen peroxide using the same and synthesis methode of the same |
-
2014
- 2014-08-22 CN CN201410418331.7A patent/CN104165876B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590164A (en) * | 2012-02-03 | 2012-07-18 | 桂林理工大学 | Method for determining hydrogen peroxide content |
| KR20130107060A (en) * | 2012-03-21 | 2013-10-01 | 아주대학교산학협력단 | Changeable color two-photon fluorescent probes for hydrogen peroxide in mitochondria and biological imaging methode of hydrogen peroxide using the same and synthesis methode of the same |
| CN103087328A (en) * | 2013-02-01 | 2013-05-08 | 北京理工大学 | Preparation method of chitosan 6-OH immobilized cyclodextrin derivative and application thereof in H2O2 detection fluorescent biosensor |
Non-Patent Citations (2)
| Title |
|---|
| ARUN SINGH PATEL等: "Silver nanoclusters in BSA template: a selective sensor for hydrogen peroxide", 《J MATER SCI》 * |
| 唐建华: "基于荧光光谱的血浆中亚甲蓝含量测定和二氧化硅强荧光纳米粒子的制备及应用", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181953A (en) * | 2015-06-26 | 2015-12-23 | 江南大学 | Grape-cluster-like nanoparticles, immune probe, and preparation method and applications of immune probe |
| CN105731534A (en) * | 2016-02-03 | 2016-07-06 | 浙江理工大学 | Method for labeling titanium dioxide nanotubes with green fluorescence |
| CN106404739A (en) * | 2016-09-07 | 2017-02-15 | 江南大学 | Surface-enhanced Raman scattering substrate as well as preparation method and application thereof |
| CN106404739B (en) * | 2016-09-07 | 2018-10-30 | 江南大学 | A kind of surface enhanced Raman scattering substrate, preparation method and applications |
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