CN104160998A - Construction method and application of male sterility line of hybrid scallops of purple scallops and Argopecten irradians - Google Patents
Construction method and application of male sterility line of hybrid scallops of purple scallops and Argopecten irradians Download PDFInfo
- Publication number
- CN104160998A CN104160998A CN201410428981.XA CN201410428981A CN104160998A CN 104160998 A CN104160998 A CN 104160998A CN 201410428981 A CN201410428981 A CN 201410428981A CN 104160998 A CN104160998 A CN 104160998A
- Authority
- CN
- China
- Prior art keywords
- scallop
- purple
- male sterile
- hybrid
- sterile line
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a construction method and application of the male sterility line of hybrid scallops of purple scallops and Argopecten irradians. The male sterility line cannot generate functional sperms, ova of the male sterility line can be normally fertilized and develop, the male sterility characteristics can be inherited to the descendant hybrid scallop strains, and the hybrid scallops of the purple scallops and the Argopecten irradians are filial generations of purple scallop ova and Argopecten irradians sperms. Firstly, a large number of purple scallop and Argopecten irradians hybrid families of the first filial generation are constructed, male sterile individuals are found out from the purple scallop and Argopecten irradians hybrid families of the first filial generation, then sperms of male parent Argopecten irradians of the male sterile individuals are used for continuous backcross breeding until males of all backcross generations are sterile and ova of all the backcross generations are fertile, and the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians can be obtained; the male sterility line and a maintainer line are used for hybridization to produce parent scallops of the male sterility line in a large scale, and the parent scallops of the male sterility line and a restorer line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians in a large scale. According to the method and application, the male sterility line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.
Description
Technical field
The invention belongs to shellfish breeding technique--scallop hybrid commodity offspring seed cultivation method, especially relates to construction method and the application of purple extra large scallop hybrid (filial generation of purple scallop ovum and bay scallop sperm) male sterile line.
Background technology
Bay scallop (Argopecten irradians irradians) originates in U.S., Atlantic bank, in nineteen eighty-two, introduces China.Bay scallop fast growth, temperature wide accommodation, strong stress resistance is applicable to the cultivation in northern China marine site very much, and current production rate and cultured area account for the more than 50% of China's scallop culture.But due to long-term inbreeding after introducing, bay scallop germplasm in breeding process is degenerated serious, show as commodity shellfish specification more and more less, in seedling cultivation process, lethality is higher, therefore scallop culture industry is in the urgent need to carrying out genetic improvement to bay scallop, and wherein crossbreeding is one of the most effective germplasm improvement method.
For this reason, inventor has introduced purple scallop (Argopecten purpuratus) from Peru first from 2007.Purple scallop is the medium-sized scallop of a kind of fast-growing type that originates in the South Pacific Ocean, all belongs to Argopecten belong to bay scallop, and chromosome number is identical, and is all hermaphroditism, but the two is complementary in aspect proterties such as Individual Size, growth and thermal adaptabilities.Therefore inventor, again by purple scallop and bay scallop are carried out to interspecific cross, turns out quadrature (purple scallop ovum and the fertilization of bay scallop sperm) and two kinds of hybridization first filial generations of reciprocal cross (bay scallop ovum and the fertilization of purple scallop sperm).The individual weight of hybridization first filial generation improves approximately 100% than the bay scallop of cultivating the same period, can grow to 7-8 centimetre then, and Second Year can continue cultivation and reaches larger specification, individual weight reaches more than 200 grams, its resistance strengthens, particularly in Shandong District, can aestivate smoothly and survive the winter, demonstrate huge industry potentiality (Wang Chunde etc., the research of 2009. purple scallops and bay scallop interspecific cross.Marine Sciences, 33 (10): 84-87; Chunde Wang etc., 2011.Introduction of the Peruvian scallop and its hybridization with the bay scallop in China, Aquaculture, 2011,310:380-387).
Although above-mentioned hybridization first filial generation can produce larger hybrid vigour, but want spread still to exist larger difficulty, this is because bay scallop and purple scallop are all hermaphroditic scallops, during breeding, discharge sperm and ovum simultaneously, the ovum of discharge is easily subject to the pollution of sperm of the same race, be difficult to obtain in a large number unfertilized egg for interspecific cross, thereby cause filial generation ratio real in obtained commodity seed greatly to reduce, thereby make the increase rate of its output greatly limited.
Research discovery, the hermaphroditism characteristic of above-mentioned two kinds of Argopecten scallops is similar to the monoecism characteristic of many crops.Hermaphrodite crops, also exist the problem of color pollination in as rice breeding, need artificial emasculation, this point is similar to the self-fertilization problem running in Argopecten irradians irradians process to purple scallop.The key addressing this problem in agricultural depends on the discovery of male sterile plants, utilize male sterile plant and its male parent continuous backcross, just can select male sterile line, maintainer and restorer, with this, build the three series mating breeding system in crop breeding, realize the large-scale production of crossbreed, thereby solve a hybrid seeding difficult problem.Up to now, in numerous crops set up three series mating breeding system both at home and abroad, and be widely used in, in the production of plant hybrid, becoming the main path that increases substantially crop yield.
But in animal breeding, there is no up to now both at home and abroad and find male sterile and femalely educate individual report, thereby also cannot set up male sterile line, maintainer and the restorer that is similar to crops, for crossbreeding.Obviously; if can build the three series mating breeding system that similarly comprises male sterile line, maintainer and restorer in animal breeding; also just can realize low cost, the scale interspecific cross of hermaphroditism type animal; improve hybridization efficiency; improve resistance and the output of breeding animal, thereby realize the important breakthrough of animal breeding technology.The seed selection of male sterile line is the core strain of three series mating breeding system, is key and the prerequisite of setting up three series mating breeding system.
Summary of the invention
The object of the invention is to realize in animal breeding, specifically in hermaphroditism type scallop, set up first the construction method of purple extra large scallop hybrid male sterile line, to overcome the deficiency of existing scallop hybrid breeding technique.
Another object of the present invention is to utilize the extra large scallop hybrid male sterile line of above-mentioned purple and maintainer hybridization to set up application method extensive, the purple extra large scallop hybrid male sterile line kind shellfish of low-cost production.
The 3rd object of the present invention is to set up application method extensive, the purple extra large scallop hybrid commodity seed of low-cost production with purple extra large scallop hybrid male sterile line and restorer hybridization.
It is undesired that above-mentioned male sterile line is that male gonad is grown, and can not produce the sperm of function, but its egg development is normal, can accept eupyrene sperm and be fertilized, and can be by its male sterile characteristic hereditary the strain to offspring.Obviously male sterile line is the core strain in three series mating system, and its Main Function is the unfertilized ovum that provides a large amount of in hybrid seedlings is commercially produced, and it is bred and depends on the maintainer supporting with it simultaneously.Male sterile line is the individual seed selection that utilizes male sterile serendipitous and ovum to educate.
Above-mentioned maintainer claims again male sterile line maintainer, when as male parent and male sterile line hybridization, can make first filial generation keep male sterile characteristic, and the Main Function of maintainer is to provide male sterile line and breeds necessary sperm.Maintainer is by constantly backcrossing with bay scallop and male sterile individuality, when backcross progeny all shows as the bay scallop strain obtaining when male sterile and ovum can be educated.Maintainer is the hermaphroditism strain that can normally breed, can breed maintainer of future generation by selfing.
Restorer claims again male sterile line restorer, and after the ovum fertilization with its sperm to male sterile line, the female and male fertile of the filial generation that produces all recovers normal.Therefore, in animal breeding, the effect of restorer is to provide the ovum fertilization of sperm and male sterile line, to realize the cultivation of good scallop hybrid commodity seed.Restorer is by use bay scallop and male sterile line kind shellfish continuous backcross, the bay scallop strain obtaining when backcross progeny all shows as hermaphroditism.Restorer is the hermaphroditism strain that can normally breed, can breed restorer of future generation by selfing.
The present invention is based on following design and practice: 1) inventor studies and found to exist in purple extra large scallop hybrid (filial generation of purple scallop ovum and bay scallop sperm) first filial generation the male sterile of 5-10% individual, these individualities do not produce activated sperm, but its ovum can be fertilized by the sperm of bay scallop, and the fertilized egg obtaining can normal development, has obtained the scallop that backcrosses, 2) research is further found, after the thus obtained scallop gonad maturity that backcrosses, approximately there is the individuality of 5-8% still for male sterile and its ovum can be fertilized by bay scallop sperm, therefore as long as the ovum of above-mentioned male sterile individuality and male parent bay scallop self progeny's sperm continuous multi-generation is backcrossed, just can select offspring is all male sterile line (female parent)-bay scallop (male parent) combination that male sterile and ovum can be educated, bay scallop strain in this combination has the ability that makes the offspring of corresponding male sterile line still keep male sterile characteristic, obtain the maintainer of male sterile line He this male sterile line of purple extra large scallop hybrid, 3) research is also found, with the ovum of male sterile individuality in bay scallop sperm and purple extra large scallop hybrid the cultivated scallop that backcrosses that backcrosses, form rear approximately 90% individuality and still show as hermaphroditism, therefore can be by setting up a plurality of methods that backcross family and carry out coordinate force test, selecting its offspring is all the male sterile line that hermaphroditism and proterties are good (female parent)-bay scallop (male parent) combination, bay scallop strain in this combination has the ability that makes male sterile line recover male fertile, obtains the restorer of purple extra large scallop hybrid male sterile line, 4) applying purple extra large scallop hybrid male sterile line and corresponding maintainer hybridization can large-scale production male sterile and the ovum kind shellfish that can educate, by these male sterile line kind shellfishes and the sperm of corresponding restorer are hybridized, can realize convenient, efficient, the purple extra large scallop hybrid commodity seed of large-scale production, and the scallop hybrid commodity seed obtaining has significant advantage aspect growth, Individual Size and temperature tolerance.
The construction method of the extra large scallop hybrid male sterile line of purple of the present invention is as follows:
1) breeding of the screening of purple extra large scallop hybrid male sterile individuality and male parent thereof: select good purple scallop and bay scallop individuality to synchronize after accelerating, with conventional bay scallop, dry in the shade and induce purple scallop and bay scallop laying eggs with thermal stimulus method, and collect respectively the ovum of purple scallop and bay scallop, induce bay scallop ejaculation simultaneously, and obtain bay scallop sperm with 500 order silk cover filterings, then in the ovum of purple scallop, add appropriate bay scallop sperm, after fertilized egg normal development, obtain purple extra large scallop hybrid (purple scallop ovum and the fertilization of bay scallop sperm) first filial generation family, be ready to use in and select male sterile individual, simultaneously by autologous sperm fertilization for the ovum of bay scallop, set up the self-fertilization family (sff) of this bay scallop.Because the individual ratio existing of male sterile in hybridization first filial generation is very low, therefore need to build a large amount of hybridization first filial generation familys, thereby find male sterile individuality for the seed selection of male sterile line.
2) seed selection of purple extra large scallop hybrid male sterile line: select the male sterile in the family of the extra large scallop hybrid of above-mentioned purple first filial generation individual, synchronize accelerating with the defect individual in male parent bay scallop self-fertilization family (sff), after gonad maturity, with conventional bay scallop, dry in the shade and induce the scallop ovulation of above-mentioned hybridization first filial generation recovery of ova with the method for thermal stimulus, induce bay scallop ejaculation simultaneously, and obtain bay scallop sperm with 500 order silk cover filterings, by the ovum of the hybridization first filial generation of above-mentioned acquisition and the fertilization of bay scallop sperm, family that collection is fertilized and the foundation of normotrophic fertilized egg backcrosses, to set up self-fertilization family (sff) for smart bay scallop self-fertilization simultaneously, the family that will backcross next year and male parent bay scallop self-fertilization family (sff) accelerating thereof, selection backcross in family still for male sterile individual with conventional bay scallop laying eggs abductive approach stimulation, lay eggs after, with its male parent bay scallop self progeny's sperm, continue to backcross, will continue selfing for smart bay scallop simultaneously and set up self-fertilization family (sff), continuous backcross seed selection after the same method every year later, until backcross progeny is all the individuality that male sterile and ovum can be educated, obtains purple extra large scallop hybrid male sterile line.Male sterile line is the core strain of three series mating breeding system; it is prerequisite; the object of setting up purple extra large scallop hybrid male sterile line is that the production for scallop hybrid seed provides a large amount of male sterile scallop kind shellfishes, thereby realize convenient, efficient, scale, obtains a large amount of unfertilized eggs.
3) expansion of purple extra large scallop hybrid male sterile line kind shellfish is numerous: the present invention is by the crossbreed purple extra large scallop hybrid male sterile line scallop of future generation of purple extra large scallop hybrid male sterile line and maintainer, thereby the expansion that realizes purple extra large scallop hybrid male sterile line kind shellfish is numerous, and male sterile line kind shellfish is produced for following purple sea scallop hybrid commodity seed.
4) production method of purple extra large scallop hybrid commodity seed: male sterile line and the restorer kind shellfish of first selecting purple extra large scallop hybrid according to the ratio of 5:1, and stimulate respectively after accelerating according to a conventional method male sterile line kind shellfish to lay eggs and the restorer kind shellfish ejaculation of laying eggs, again the smart ovum mixed liquor of this restorer is obtained to the seminal fluid of restorer with 500 order silk cover filterings, the seminal fluid of restorer is added in spawning pond and stirred, and add the amount of sperm to have 5-6 sperm to be advisable around with each ovum, finally after hatching, obtain purple extra large scallop hybrid commodity seed according to a conventional method.
Male sterile line construction method of the present invention is also applied to the breeding that the Argopecten such as extra large purple scallop hybrid (filial generation of bay scallop ovum and purple scallop sperm), purple black scallop hybrid (filial generation of purple scallop ovum and Gulf of Mexico scallop sperm) and violet black scallop hybrid (filial generation of Gulf of Mexico scallop ovum and purple scallop sperm) belong to scallop hybrid, and is applied to set up the large-scale method for producing of these scallop hybrid commodity seeds.
Method of the present invention is also applied to the interspecific cross breeding of other hermaphroditism type scallops, and for example Pecten belongs to the interspecific cross of scallop, and basic skills of the present invention is also applicable to the interspecific cross breeding of other hermaphroditism type invertebrates.
The purple sea scallop hybrid male sterile line that the present invention builds is set up first in the animal kingdom, is core and the prerequisite of animal three series mating breeding system, should belong to the important breakthrough in animal breeding technology.The present invention has effectively overcome autogamous obstacle in hermaphroditism scallop crossover process; realize low cost and scale and obtained filial generation completely; therefore can make full use of hybrid vigour; reached the characteristic that makes cultivated purple sea scallop hybrid commodity seed simultaneously there is bay scallop and purple scallop; aspect growth, Individual Size and temperature tolerance, than bay scallop, there is significant advantage; therefore can be in northern sea area as the marine sites such as Qingdao, Dalian successfully cultivate, and improve more than 40% when the comparable bay scallop of annual production.
Embodiment
The present invention has built purple extra large scallop hybrid male sterile line first, and has set up the method with the purple extra large scallop hybrid male sterile line kind shellfish of male sterile line large-scale production and purple extra large scallop hybrid commodity seed, and concrete steps are as follows:
Embodiment 1
1) breeding of the screening of purple extra large scallop hybrid male sterile individuality and male parent thereof: First Year is selected the purple scallop of 50 sexual gland fully-developeds and 50 bay scallops, dry in the shade stimulation was placed on each scallop in the independent container that adds in advance 23 ℃ of seawater it is laid eggs after 30 minutes, ejaculation, collect separately the ovum of each purple scallop and the sperm of each bay scallop and ovum, in the ovum suspension of each purple scallop, add a small amount of seminal fluid from single bay scallop, stirring mixes smart ovum, micro-Microscopic observation makes each ovum have 5-6 sperm around, and fertilized egg is placed under dark surrounds and is hatched, obtain hybridization first filial generation, purple sea family.With said method, set up at least 20 such hybridization familys, bay scallop essence ovum for smart is mixed to fertilization simultaneously, set up the bay scallop self-fertilization family (sff) of respective numbers, after carrying out larvae cultivation, keep a full stand of seedings and form according to conventional Process of Rearing Scallop Seeds method, for the screening of male sterile individuality.
2) seed selection of purple extra large scallop hybrid male sterile line: Second Year is hybridized purple sea after the family accelerating of first filial generation spring, select 50 male sterile individualities that proterties is good, with conventional bay scallop laying eggs abductive approach, stimulate and lay eggs, with the sperm of its male parent bay scallop, be fertilized respectively, collect normal fertilization, the fertilized egg of growing foundation family that backcrosses, collect normal fertilization, the fertilized egg of growing foundation family that backcrosses, to provide the bay scallop of sperm from numerous self-fertilization family (sff) of setting up simultaneously, set up at least 20 familys that backcross with corresponding bay scallop self-fertilization family (sff) and carry out larvae cultivation according to conventional bay scallop seedling-cultivating method, keep a full stand of seedings, form, the 3rd year spring, after the family that will backcross and bay scallop self-fertilization family (sff) accelerating, select 50 male sterile individualities that backcross in family that proterties is good, with self progeny's sperm of its male parent bay scallop, backcross, collect the fertilized egg continuation of normal fertilization, growth and set up the family that backcrosses, continue to breed the self-fertilization family (sff) of bay scallop simultaneously, select to set up 20 backcross family and corresponding bay scallop self-fertilization family (sff)s thereof that above proterties is good, the male sterile scallop that later annual selection traits is after the same method good and the self-fertilization family (sff) of its male parent bay scallop backcross, until all individualities of backcross progeny all show as, proterties is good, male sterile and ovum can be educated, and obtains purple extra large scallop hybrid male sterile line.
3) expanding propagation method of purple extra large scallop hybrid male sterile line kind shellfish: expand the extra large scallop hybrid male sterile line of numerous purple scallop with purple extra large scallop hybrid male sterile line and the hybridization of maintainer kind shellfish every year, numerous restorer is expanded in the selfing of bay scallop seedling-cultivating method routinely simultaneously.
4) production method of purple extra large scallop hybrid commodity seed: select after purple extra large scallop hybrid male sterile line and restorer kind shellfish accelerating according to the ratio of 5:1 when grow seedlings spring, spawning pond moderate stimulation male sterile line kind shellfish, lay eggs, in bucket moderate stimulation restorer kind shellfish ejaculation, and with 500 order silk cover filterings, obtain the restorer bay scallop seminal fluid that does not contain ovum; The seminal fluid of restorer is evenly splashed and stirred in spawning pond and immediately, and fertilization situation is examined under a microscope in sampling at any time, and seminal fluid addition has 5-6 sperm to be advisable around with each ovum; Fertilized egg is hatched according to conventional method, obtains purple extra large scallop hybrid business seed after growth.
Embodiment 2
In by the end of March, 2008, gets the purple scallop of gonadal maturation, obtains purple scallop ovum after thermal stimulus, after the sperm insemination with bay scallop, obtains purple scallop and Argopecten irradians irradians first filial generation seed and form.Spring in 2009, by the scallop of therefrom selecting individual great Qie Jinci district to grow after the scallop accelerating of above-mentioned hybridization first filial generation, after drying in the shade 30 minutes, scallop being placed in to the container moderate stimulation that fills 23 ℃ of seawater lays eggs, with the sperm from a plurality of bay scallops, mix insemination, cultivate the backcross population of first filial generation Zi Hai scallop hybrid and bay scallop.This backcross population major part after spring in 2010 accelerating shows as hermaphroditism, but also has the individuality of <10% still to show as male sterile.
Continuation backcrosses above-mentioned male sterile individuality with its male parent bay scallop self progeny's sperm, through a generation, just can obtain offspring at the most for seed selection is all male sterile line (female parent)-maintainer (male parent) combination that male sterile and ovum can be educated, thereby obtains respectively male sterile line and maintainer.After gonad maturity accelerating, with the conventional bay scallop laying eggs abductive approach male sterile line scallop of hastening parturition, can obtain a large amount of unfertilized ovums; used the sperm fertilization of maintainer, just can be obtained on a large scale the male sterile line kind shellfish producing for purple extra large scallop hybrid commodity seed.
Finally, by after the male sterile line kind shellfish accelerating of the purple sea scallop hybrid of above-mentioned acquisition, with conventional bay scallop induced spawning method, obtain a large amount of unfertilized ovums, used the sperm fertilization of restorer, purple extra large scallop hybrid commodity seed just can be mass-produced.
Embodiment 3
Spring in 2010, male sterile individuality in the purple scallop ovum of selecting set up for 2009 and bay scallop sperm hybridization first filial generation stimulates and lays eggs after accelerating, and the sperm of use bay scallop is fertilized, and has set up 12 familys forming of backcrossing.Find after accelerating spring in 2011, the most of individualities of 11 familys wherein show as hermaphroditism, and grow in the individual Jin Ci of the major part district of 1 family wherein, a small amount of individual sexual gland agensis.
Using such method, backcrosses the ovum of single male sterile individuality wherein and the sperm of single bay scallop to set up some familys that backcross, and will set up corresponding self-fertilization family (sff) for smart bay scallop selfing.The backcross gonad development situation of family of Second Year inspection, if backcross, family has male sterile individuality, further with the sperm of its male parent self-fertilization family (sff), detect the fertility of its ovum, and continue to set up backcross family and male parent bay scallop self-fertilization family (sff), carry out seed selection of future generation; Through a generation or after how for seed selection, when backcross progeny is all male sterile and ovum can educate time, male sterile line and corresponding maintainer thereof have just been obtained.
Embodiment 4
2013 and 2014, the purple scallop of utilize introducing and bay scallop are built together and have been found 18 of the hybridization first filial generation familys of purple scallop ovum and bay scallop sperm, and set up the self-fertilization family (sff) for smart bay scallop, according to the method for embodiment 1, male sterile family and corresponding maintainer and the restorer that can in 1-3, select purple extra large scallop hybrid, realize commercially producing of purple extra large scallop hybrid seed.
Embodiment 5
The present invention is for the construction and application of extra large purple scallop hybrid male sterile line
The construction method of the male sterile line that the present invention sets up is also applied to the breeding of extra large purple scallop hybrid (filial generation of bay scallop ovum and purple scallop sperm), and is applied to set up the large-scale method for producing of extra large purple scallop hybrid commodity seed.
First select bay scallop and the synchronous accelerating of purple scallop and stimulate lay eggs simultaneously, ejaculation, collect respectively the ovum of bay scallop and the sperm of purple scallop and ovum, the sperm fertilization of the ovum of bay scallop and purple scallop is set up to purple hybridization first filial generation, sea family, be used for selecting male sterile individual, the sperm of purple scallop and ovum are mixed to fertilization simultaneously, set up purple scallop self-fertilization family (sff); Second Year is selected male sterile individuality from the family of purple hybridization first filial generation, sea, sperm and its fertilization with purple scallop, if the family that backcrosses is set up in fertilized egg normal development, for continuing seed selection male sterile line, the purple scallop self-fertilization of male parent is set up to self-fertilization family (sff) simultaneously; Through generation generation selection at the most, when backcross progeny all shows as male sterile, just obtained male sterile line and the corresponding maintainer of extra large purple scallop hybrid like this.
With the male sterile line ovum of the purple scallop hybrid in above-mentioned sea and the hybridization of the sperm of maintainer, can realize male sterile kind shellfish efficient, convenient, the extra large purple scallop hybrid of large-scale production; With the male sterile kind shellfish of the purple scallop hybrid in sea and the hybridization of the sperm of restorer, can realize efficient, convenient, the extra large purple scallop hybrid seed of large-scale production.
Embodiment 6
The present invention is for the construction and application of purple black scallop hybrid male sterile line
The construction method of the male sterile line that the present invention sets up is also applied to purple black scallop hybrid (filial generation of purple scallop ovum and Gulf of Mexico scallop sperm) breeding, and is applied to set up the large-scale method for producing of purple black scallop hybrid commodity seed.
First select purple scallop and the synchronous accelerating of Gulf of Mexico scallop and stimulate lay eggs simultaneously, ejaculation, collect respectively the ovum of purple scallop and sperm and the ovum of Gulf of Mexico scallop, the sperm fertilization of the ovum of purple scallop and Gulf of Mexico scallop is set up to purple China ink hybridization first filial generation family, be used for selecting male sterile individual, the sperm of Gulf of Mexico scallop and ovum are mixed to fertilization simultaneously, set up Gulf of Mexico scallop self-fertilization family (sff); Second Year is selected male sterile individuality from the family of purple China ink hybridization first filial generation, sperm and its fertilization with Gulf of Mexico scallop, if the family that backcrosses is set up in fertilized egg normal development, for continuing seed selection male sterile family, the scallop self-fertilization of the male parent Gulf of Mexico is set up to self-fertilization family (sff) simultaneously; Through generation generation selection at the most, when backcross progeny all shows as male sterile, just obtained male sterile line and the corresponding maintainer of purple black scallop hybrid like this.
With the male sterile line ovum of the black scallop hybrid of above-mentioned purple and the hybridization of the sperm of maintainer, can realize efficient, convenient, the purple black scallop hybrid male sterile kind shellfish of large-scale production; Sperm hybridization by the black scallop hybrid male sterile of purple kind shellfish and restorer, can realize efficient, convenient, the purple black scallop hybrid seed of large-scale production.
Embodiment 7
The present invention is for the construction and application of violet black scallop hybrid male sterile line
The construction method of the male sterile line that the present invention sets up is also applied to violet black scallop hybrid (filial generation of Gulf of Mexico scallop ovum and purple scallop sperm) breeding practice, and is applied to set up the large-scale method for producing of violet black scallop hybrid commodity seed.
First select purple scallop and the synchronous accelerating of Gulf of Mexico scallop and stimulate lay eggs simultaneously, ejaculation, collect respectively the ovum of Gulf of Mexico scallop and the sperm of purple scallop and ovum, the sperm fertilization of the ovum of Gulf of Mexico scallop and purple scallop is set up to violet black hybridization first filial generation family, be used for selecting male sterile individual, the sperm of purple scallop and ovum are mixed to fertilization simultaneously, set up purple scallop self-fertilization family (sff); Second Year is selected male sterile individuality from the family of violet black hybridization first filial generation, sperm and its fertilization with purple scallop, if the family that backcrosses is set up in fertilized egg normal development, for continuing seed selection male sterile family, the purple scallop self-fertilization of male parent is set up to self-fertilization family (sff) simultaneously; Through generation generation selection at the most, when backcross progeny all shows as male sterile, just obtained male sterile line and the corresponding maintainer thereof of violet black scallop hybrid like this.
With the male sterile line ovum of above-mentioned violet black scallop hybrid and the hybridization of the sperm of maintainer, can realize efficient, convenient, large-scale production violet black scallop hybrid male sterile kind shellfish; Sperm hybridization by violet black scallop hybrid male sterile kind shellfish and restorer, can realize efficient, convenient, large-scale production violet black scallop hybrid seed.
Claims (10)
1. the extra large scallop hybrid male sterile line of purple, it is characterized in that, this male sterile line is the sperm that can not produce function, but its ovum energy normal fertilization, growth, and can be by its male sterile characteristic hereditary the scallop hybrid strain to offspring, wherein purple extra large scallop hybrid is the filial generation of purple scallop ovum and bay scallop sperm.
2. the construction method of the purple of claim 1 sea scallop hybrid male sterile line, is characterized in that:
1) breeding of the screening of purple extra large scallop hybrid male sterile individuality and male parent thereof: first build 20 above purple extra large scallop hybrid first filial generation familys, set up for smart male parent bay scallop self-fertilization family (sff), for the seed selection of following male sterile line simultaneously;
2) seed selection of purple extra large scallop hybrid male sterile line: Second Year is selected 50 male sterile individualities from the family of the extra large scallop hybrid of above-mentioned purple first filial generation, after accelerating, stimulate its ovulation, sperm fertilization with defect individual in the male parent bay scallop self-fertilization family (sff) of this male sterile individuality, fertilized egg foundation of collecting normal development backcrosses family more than 20, will set up self-fertilization family (sff) for smart bay scallop self-fertilization simultaneously; After selecting next year to backcross in family still as male sterile individual 50 acceleratings and stimulating and lay eggs, with the male parent bay scallop self progeny's of this male sterile individuality sperm, continue to backcross, set up 20 familys that backcross above, will continue selfing for smart bay scallop simultaneously and set up self-fertilization family (sff); Continuous backcross seed selection after the same method every year later, until backcross progeny is all the individuality that male sterile and ovum can be educated, obtains purple extra large scallop hybrid male sterile line; Meanwhile, by above-mentioned, can make male sterile line offspring continue to keep the bay scallop self-fertilization family (sff) of male sterile characteristic numerous with conventional Process of Rearing Scallop Seeds method selfing expansion, obtain the maintainer of this male sterile line.
3) expansion of male sterile line kind shellfish is numerous: the crossbreed purple extra large scallop hybrid male sterile line scallop of future generation by above-mentioned male sterile line and maintainer, thus it is numerous to realize the expansion of purple extra large scallop hybrid male sterile line.
3. the purple of the claims 1 and claim 2 sea scallop hybrid male sterile line is applied to the purple extra large scallop hybrid male sterile line kind shellfish of large-scale production; with purple extra large scallop hybrid male sterile line and corresponding maintainer hybridization, the purple extra large scallop hybrid male sterile line kind shellfish of the next generation that can educate with propagation in scale male sterile and ovum.
4. the purple of the claims 1 and claim 2 sea scallop hybrid male sterile line is applied to produce purple extra large scallop hybrid commodity seed, with the ovum of purple extra large scallop hybrid male sterile line and the sperm hybridization of corresponding restorer, obtain purple extra large scallop hybrid commodity seed.
5. the construction method of the purple sea scallop hybrid male sterile line that claim 2 is set up is also applied to the breeding of each following scallop hybrid:
(1) the purple scallop hybrid in sea of cultivating with bay scallop ovum and the fertilization of purple scallop sperm;
(2) the purple China ink hybridization fan of cultivating with purple scallop ovum and the fertilization of Gulf of Mexico scallop sperm
Shellfish;
(3) the violet black hybridization fan of cultivating with Gulf of Mexico scallop ovum and the fertilization of purple scallop sperm
Shellfish.
6. the application method of the purple sea scallop hybrid male sterile line that claim 3 and claim 4 are set up is also applied to the breeding of each following scallop hybrid:
(1) the purple scallop hybrid in sea of cultivating with bay scallop ovum and the fertilization of purple scallop sperm;
(2) the purple China ink hybridization fan of cultivating with purple scallop ovum and the fertilization of Gulf of Mexico scallop sperm
Shellfish;
(3) the violet black hybridization fan of cultivating with Gulf of Mexico scallop ovum and the fertilization of purple scallop sperm
Shellfish.
7. the construction method of the purple sea scallop hybrid male sterile line that claim 2 is set up is also applied to other hermaphroditism type scallops, and for example Argopecten belongs to and the interspecific cross breeding of Pecten genus scallop.
8. the purple sea scallop hybrid male sterile line application method that claim 3 and claim 4 are set up is also applied to other hermaphroditism type scallops, and for example Argopecten belongs to and the interspecific cross breeding of Pecten genus scallop.
9. the basic construction method of the purple sea scallop hybrid male sterile line that claim 2 is set up is also applied to the interspecific cross breeding of other hermaphroditism type invertebrates.
10. the application method of the purple sea scallop hybrid male sterile line that claim 3 and claim 4 are set up is also applied to the interspecific cross breeding of other hermaphroditism type invertebrates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410428981.XA CN104160998B (en) | 2014-08-27 | 2014-08-27 | The construction method of purple extra large scallop hybrid male sterile line and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410428981.XA CN104160998B (en) | 2014-08-27 | 2014-08-27 | The construction method of purple extra large scallop hybrid male sterile line and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104160998A true CN104160998A (en) | 2014-11-26 |
| CN104160998B CN104160998B (en) | 2016-02-24 |
Family
ID=51904904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410428981.XA Active CN104160998B (en) | 2014-08-27 | 2014-08-27 | The construction method of purple extra large scallop hybrid male sterile line and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104160998B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793969A (en) * | 2022-05-11 | 2022-07-29 | 中国科学院烟台海岸带研究所 | Method for backcrossing and producing seeds of hybrid scallop and purple scallop |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
| CN117121853A (en) * | 2022-09-19 | 2023-11-28 | 中国海洋大学 | Preparation method of scallop solitary haploid embryo |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703017A (en) * | 2009-07-31 | 2010-05-12 | 青岛农业大学 | Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings |
| CN102308770B (en) * | 2011-08-05 | 2012-07-18 | 青岛农业大学 | Breeding method for new marine backcrossed scallop variety |
| CN102301968B (en) * | 2011-08-05 | 2012-08-22 | 青岛农业大学 | Method for cultivating new variety of hybridized and backcrossed Argopecten purpuratus |
-
2014
- 2014-08-27 CN CN201410428981.XA patent/CN104160998B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703017A (en) * | 2009-07-31 | 2010-05-12 | 青岛农业大学 | Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings |
| CN102308770B (en) * | 2011-08-05 | 2012-07-18 | 青岛农业大学 | Breeding method for new marine backcrossed scallop variety |
| CN102301968B (en) * | 2011-08-05 | 2012-08-22 | 青岛农业大学 | Method for cultivating new variety of hybridized and backcrossed Argopecten purpuratus |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793969A (en) * | 2022-05-11 | 2022-07-29 | 中国科学院烟台海岸带研究所 | Method for backcrossing and producing seeds of hybrid scallop and purple scallop |
| CN117121853A (en) * | 2022-09-19 | 2023-11-28 | 中国海洋大学 | Preparation method of scallop solitary haploid embryo |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
| CN116391654B (en) * | 2023-03-30 | 2023-11-03 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104160998B (en) | 2016-02-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102318571B (en) | Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus | |
| CN102301968B (en) | Method for cultivating new variety of hybridized and backcrossed Argopecten purpuratus | |
| CN102308770B (en) | Breeding method for new marine backcrossed scallop variety | |
| CN102301969B (en) | Method for cultivating hybridized and backcrossed marine product fries | |
| CN102301971B (en) | Method for breeding novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians | |
| CN102301970B (en) | Method for cultivating hybridized and backcrossed Argopecten product fries | |
| CN104186388B (en) | The construction method of the purple scallop hybrid three series mating breeding system in sea and application | |
| CN104160996B (en) | The construction method of purple black scallop hybrid maintainer and application | |
| CN104186386B (en) | The construction method of violet black scallop hybrid three series mating breeding system and application | |
| CN104186389B (en) | The construction method of purple black scallop hybrid three series mating breeding system and application | |
| CN106234267A (en) | A kind of miscellaneous sea backcrosses the selection of the low temperature resistant strain of scallop | |
| CN104160998B (en) | The construction method of purple extra large scallop hybrid male sterile line and application | |
| CN104206325B (en) | The construction method of purple extra large scallop hybrid three series mating breeding system and application | |
| CN104304098B (en) | The construction method of purple extra large scallop hybrid restorer and application | |
| CN104160999B (en) | The construction method of purple black scallop hybrid restorer and application | |
| CN106069956A (en) | A kind of selection of the sea low temperature resistant strain of the miscellaneous scallop that backcrosses | |
| CN104206326B (en) | The construction method of violet black scallop hybrid restorer and application | |
| CN104186384B (en) | The construction method of the purple scallop hybrid male sterile line in sea and application | |
| CN104206327B (en) | The construction method of purple extra large scallop hybrid maintainer and application | |
| CN104186385B (en) | The construction method of violet black scallop hybrid male sterile line and application | |
| CN104255587B (en) | The construction method of the purple scallop hybrid maintainer in sea and application | |
| CN104160997B (en) | The construction method of purple black scallop hybrid male sterile line and application | |
| CN104221959B (en) | The construction method of violet black scallop hybrid maintainer and application | |
| CN104186387B (en) | The construction method of the purple scallop hybrid restorer in sea and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Chunde Inventor after: Ma Bin Inventor before: Wang Chunde Inventor before: Sun Kuifu Inventor before: Ma Bin |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180418 Address after: 264006 5, Changjiang Road, Yantai economic and Technological Development Zone, Shandong, 5 Patentee after: Yantai sea spring aquaculture technology Co., Ltd. Address before: 266101 West Wing 1702, building 1, Zhuzhou Road, Laoshan District, Qingdao, Shandong, 1702 Patentee before: QINGDAO OCEANWIDE BIOTECHNOLOGY CO., LTD. |