CN104140982A - Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs - Google Patents
Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs Download PDFInfo
- Publication number
- CN104140982A CN104140982A CN201410384330.5A CN201410384330A CN104140982A CN 104140982 A CN104140982 A CN 104140982A CN 201410384330 A CN201410384330 A CN 201410384330A CN 104140982 A CN104140982 A CN 104140982A
- Authority
- CN
- China
- Prior art keywords
- streptomycete
- preventing
- streptomyces
- willow
- treating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs, and relates to a preparation method of the fermentation liquor. The method mainly solves the technical problems that chemical pesticides are unsafe, and microorganism sourced pesticide is few in kind. The method comprises the steps that (1) excitation cultivation of strains is carried out, (Streptomyces sp.) Syjd3 is shifted to gause first solid media for cultivation to obtain activated (Streptomyces sp.) Syjd3; (2) the activated (Streptomyces sp.) Syjd3 is inoculated to the gause first solid media, the gause first solid media after the inoculation are put into a shake cultivation box for cultivation, and the streptomyces fermentation liquor for preventing and treating the alamo grey speck disease germs is obtained. The streptomyces fermentation liquor has an inhibition function on the alamo grey speck disease germs, and also has certain inhibition functions on alamo leaf blight germs, fusarium solani, wheat root rot germs, curvularia lunata and rice bakanae pathogen.
Description
Technical field
The present invention relates to a kind of preparation method of fermented liquid.
Background technology
Willow graywall is by willow Cercospora Sojina Hara (Sporocadus populinus (Bres.) Orsenigo, one of Rodondi & B.Sutton=Coryneum populinum Bres.) one harm poplar leaf leaf diseases of causing, generation area is large, harm is serious, mainly occur on the blade and treetop of treelet, seedling, blade is come off ahead of time, cadre's ulcer, tender top drying top, increment lowers, when serious, can cause plant death, transplanting survival rate reduces, to willow grow seedlings and afforest threaten very large.
Plant diseases is one of important factor threatening agriculture production, using at present chemical pesticide is the main method of controlling Plant diseases, but not only contaminate environment of the life-time service of chemical pesticide, destroy the eubiosis, and residual people, animal are had to harm, and make pathogenic bacteria produce resistance, therefore the biological control of Plant diseases research more and more comes into one's own.Plant endogenesis actinomycetes are the microorganisms that live in plant health organization internal, and plant host and and other microorganisms of its symbiosis between have close relationship, be a novel bacterial of biological control.Endophyte of plant has caused plant protection scholar, microbiologist, ecologist's etc. extensive concern in recent years; progressively become the focus of domestic and international research; research both at home and abroad all shows that endophyte has potential application to controlling plant diseases and exploitation is worth, and therefore finding control willow Cercospora Sojina Hara Antagonistic Fungi is one of important controlling way.The present invention has carried out suppressing the research of plant pathogenic fungi effect to this strain fermentation meta-bolites.Further development and exploitation that this invention is microbial pesticide provide good starting strain.
Summary of the invention
The present invention is that to solve chemical pesticide dangerous, the technical problem that microbial pesticide kind is less, thus a kind of preparation method of the streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara is provided.
The preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara of the present invention carries out according to the following steps:
One, the activation culture of bacterial classification: by streptomycete (Streptomyces sp.) Syjd3 inoculation Gause I solid medium, be to cultivate 4～6d under 27～29 DEG C of conditions in temperature, obtain streptomycete (Streptomyces sp.) Syjd3 of activation;
Two, fermented liquid is cultivated: to the Gause I liquid nutrient medium that packs 300mL in 500mL triangular flask into, then autoclaving 20～30min at 110～121 DEG C of temperature, after cooling, under aseptic condition, streptomycete (Streptomyces sp.) Syjd3 of the step 1 of picking 2～3 transfering loops activation is inoculated in Gause I liquid nutrient medium, then putting into shaking table incubator cultivates, shaking table temperature is 27～29 DEG C, shaking table revolution is 180～230 revs/min, fermented incubation time is 7～15d, obtains preventing and treating the streptomycete fermentation liquid of willow Cercospora Sojina Hara;
Wherein, described Gause I solid medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4, 20g agar and 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described Gause I liquid nutrient medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5gMgSO
4, 0.01g FeSO
4with 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described streptomycete (Streptomyces sp.) Syjd3, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on June 17th, 2014, and deposit number is CGMCC No.9355.
The present invention includes following beneficial effect:
1, the invention provides the method for streptomycete Syjd3 fermentation acquisition active metabolite; This meta-bolites can be applicable to the control of fungal diseases of plants, has solved chemical pesticide dangerous, and the problem that microbial pesticide kind is less, for the exploitation of agricultural bacteriocide has increased new approach.
2, streptomycete fermentation liquid of the present invention has restraining effect to willow Cercospora Sojina Hara, and willow leaf spoting bacteria, Fusarinm solani, Root Rot of Wheat germ, maize Curvularia leaf spot fungi and fusarium moniliforme are also had to certain restraining effect.
Brief description of the drawings
Fig. 1 is the growth conditions figure that does not add the willow Cercospora Sojina Hara of streptomycete fermentation liquid;
Fig. 2 is the growth conditions figure that adds the willow Cercospora Sojina Hara after the test one streptomycete fermentation liquid obtaining.
Embodiment
Embodiment one: the preparation method of a kind of streptomycete fermentation liquid of present embodiment carries out according to the following steps:
One, the activation culture of bacterial classification: by streptomycete (Streptomyces sp.) Syjd3 inoculation Gause I solid medium, be to cultivate 4～6d under 27～29 DEG C of conditions in temperature, obtain streptomycete (Streptomyces sp.) Syjd3 of activation;
Two, fermented liquid is cultivated: to the Gause I liquid nutrient medium that packs 300mL in 500mL triangular flask into, then autoclaving 20～30min at 110～121 DEG C of temperature, after cooling, under aseptic condition, streptomycete (Streptomyces sp.) Syjd3 of the step 1 of picking 2～3 transfering loops activation is inoculated in Gause I liquid nutrient medium, then putting into shaking table incubator cultivates, shaking table temperature is 27～29 DEG C, shaking table revolution is 180～230 revs/min, fermented incubation time is 7～15d, obtains preventing and treating the streptomycete fermentation liquid of willow Cercospora Sojina Hara;
Wherein, described Gause I solid medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4, 20g agar and 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described Gause I liquid nutrient medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4with 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described streptomycete (Streptomyces sp.) Syjd3, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on June 17th, 2014, and deposit number is CGMCC No.9355.
Present embodiment comprises following beneficial effect:
1, present embodiment provides streptomycete Syjd3 fermentation to obtain the method for active metabolite; This meta-bolites can be applicable to the control of fungal diseases of plants, has solved agricultural chemicals dangerous, and the problem that microbial pesticide kind is less, for the exploitation of agricultural bacteriocide has increased new approach.
2, present embodiment streptomycete fermentation liquid has restraining effect to willow Cercospora Sojina Hara, and willow leaf spoting bacteria, Fusarinm solani, Root Rot of Wheat germ, maize Curvularia leaf spot fungi and fusarium moniliforme are also had to certain restraining effect.
Embodiment two: present embodiment is different from embodiment one: be to cultivate 4d under 28 DEG C of conditions in temperature in step 1.Other is identical with embodiment one.
Embodiment three: present embodiment is from specifically mode one or two is different: in step 2 at 121 DEG C of temperature autoclaving 20min.Other is identical with embodiment one or two.
Embodiment four: present embodiment is from specifically one of mode one to three is different: in step 2, shaking table temperature is 28 DEG C.Other is identical with one of embodiment one to three.
Embodiment five: present embodiment is from specifically one of mode one to four is different: in step 2, shaking table revolution is 200 revs/min.Other is identical with one of embodiment one to four.
Embodiment six: present embodiment is from specifically one of mode one to five is different: in step 2, fermented incubation time is 10d.Other is identical with one of embodiment one to five.
By following verification experimental verification beneficial effect of the present invention:
Test one: the preparation method of a kind of streptomycete fermentation liquid of this test realizes according to the following steps:
One, the activation culture of bacterial classification: by streptomycete (Streptomyces sp.) Syjd3 inoculation Gause I solid medium, be to cultivate 4d under 28 DEG C of conditions in temperature, obtain streptomycete (Streptomyces sp.) Syjd3 of activation;
Two, fermented liquid is cultivated: to the Gause I liquid nutrient medium that packs 300mL in 500mL triangular flask into, then autoclaving 20min at 121 DEG C of temperature, after cooling, under aseptic condition, streptomycete (Streptomyces sp.) Syjd3 of the step 1 of picking 3 transfering loops activation is inoculated in Gause I liquid nutrient medium, then putting into shaking table incubator cultivates, shaking table temperature is 28 DEG C, shaking table revolution is 200 revs/min, fermented incubation time is 10d, obtains preventing and treating the streptomycete fermentation liquid of willow Cercospora Sojina Hara;
Wherein, described Gause I solid medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4, 20g agar and 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described Gause I liquid nutrient medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4with 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described streptomycete (Streptomyces sp.) Syjd3, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on June 17th, 2014, and deposit number is CGMCC No.9355.
Spore experiment by the test one streptomycete fermentation liquid obtaining for willow Cercospora Sojina Hara (S.populinus), method is: adopt sessile drop method to carry out spore germination experiment, with sterilized water in contrast, repeat 3 times, 5 visuals field of each observation, record spore germination number after 48h.
Inhibition of germination=(contrast spore germination rate-processing spore germination rate)/contrast spore germination rate × 100%
During to 48h, the streptomycete fermentation liquid that this test obtains is 88.46% to the spore inhibiting rate of willow Cercospora Sojina Hara (S.populinus).
The pure culture biscuits involvng inoculation of willow Cercospora Sojina Hara 7mm, in PDA culture dish central authorities, is cultivated to the hole of beating 7mm in distance for examination bacterium cake zygomorphy 1cm place respectively after 16d, the streptomycete fermentation liquid of crossing 0.22um membrane filtration is filled, be placed at 22 DEG C and cultivate, blank in contrast, repeats 3 times, measures antibacterial band diameter.During to 12d, do not add streptomycete fermentation liquid willow Cercospora Sojina Hara growth conditions figure as shown in Figure 1; Add the growth conditions figure of the willow Cercospora Sojina Hara after the streptomycete fermentation liquid that this test obtains as shown in Figure 2; Can be found out by Fig. 1 and Fig. 2, the streptomycete fermentation liquid that this test obtains is inhibited to the spore of willow Cercospora Sojina Hara (S.populinus), and antibacterial band diameter reaches 5.75mm.
Adopt agar punch method to detect the restraining effect of streptomycete fermentation liquid to other plant pathogenic bacteria, finding also has certain restraining effect to willow leaf spoting bacteria (Alternaria alternata), Fusarinm solani (Fusarium solani), Root Rot of Wheat germ (Drechslera sorokiniana), maize Curvularia leaf spot fungi (Curvularia lunata), fusarium moniliforme (Fusarium moniliforme), and experimental result is as follows.
| Indicator | Antibacterial band diameter (mm) |
| Willow leaf spoting bacteria | 5.1 |
| Fusarium solani | 4.0 |
| Root Rot of Wheat germ | 4.5 |
| Maize Curvularia leaf spot fungi | 4.7 |
| Fusarium moniliforme | 5.5 |
Described willow Cercospora Sojina Hara (S.populinus) is at " 5 kinds of Medicinal Herbs Extracts are to the research of willow graywall bacteriostatic activity " (Liu Ying, Liaoning forestry science and technology, the 3rd phase in 2012) in open, willow leaf spoting bacteria (Alternaria alternata) (is counted red virtue etc. in " impact of Lactarius vellereus fermenting liquor extract on several important enzyme activity of willow leaf spoting bacteria ", Beijing Forestry University's journal, in July, 2009) in open, Fusarinm solani (Fusarium solani) is at " research of Xinjiang elm bark beetle association fungi " (Zhu Xiaomei, Northeast Forestry University's academic dissertation, 2011) in open, above 3 strain bacterium are located to obtain by the author of article respectively.
Described Root Rot of Wheat germ (buy from Inst. of Plant Protection, Heilongjiang Agricultural Science Academy by Drechslera sorokiniana, maize Curvularia leaf spot fungi (Curvularia lunata), fusarium moniliforme (Fusarium moniliforme).
This patent is to subsidize (numbering 2013AA102701) by " 863 " planning item.
Claims (6)
1. prevent and treat a preparation method for the streptomycete fermentation liquid of willow Cercospora Sojina Hara, it is characterized in that the preparation method of the streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara carries out according to the following steps:
One, the activation culture of bacterial classification: by streptomycete (Streptomyces sp.) Syjd3 inoculation Gause I solid medium, be to cultivate 4～6d under 27～29 DEG C of conditions in temperature, obtain streptomycete (Streptomyces sp.) Syjd3 of activation;
Two, fermented liquid is cultivated: to the Gause I liquid nutrient medium that packs 300mL in 500mL triangular flask into, then autoclaving 20～30min at 110～121 DEG C of temperature, after cooling, under aseptic condition, streptomycete (Streptomyces sp.) Syjd3 of the step 1 of picking 2～3 transfering loops activation is inoculated in Gause I liquid nutrient medium, then putting into shaking table incubator cultivates, shaking table temperature is 27～29 DEG C, shaking table revolution is 180～230 revs/min, fermented incubation time is 7～15d, obtains preventing and treating the streptomycete fermentation liquid of willow Cercospora Sojina Hara;
Wherein, described Gause I solid medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5g MgSO
4, 0.01g FeSO
4, 20g agar and 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described Gause I liquid nutrient medium is by 20g Zulkovsky starch, 1g KNO
3, 0.5g NaCl, 0.5g K
2hPO
4, 0.5gMgSO
4, 0.01g FeSO
4with 1000mL water composition, pH is 7.2～7.6,121 DEG C of sterilizings 20 minutes;
Described streptomycete (Streptomyces sp.) Syjd3, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on June 17th, 2014, and deposit number is CGMCC No.9355.
2. the preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara according to claim 1, is characterized in that in step 1 being to cultivate 4d under 28 DEG C of conditions in temperature.
3. the preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara according to claim 1, is characterized in that in step 2 autoclaving 20min at 121 DEG C of temperature.
4. the preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara according to claim 1, is characterized in that in step 2, shaking table temperature is 28 DEG C.
5. the preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara according to claim 1, is characterized in that in step 2, shaking table revolution is 200 revs/min.
6. the preparation method of a kind of streptomycete fermentation liquid of preventing and treating willow Cercospora Sojina Hara according to claim 1, is characterized in that in step 2, fermented incubation time is 10d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410384330.5A CN104140982A (en) | 2014-08-06 | 2014-08-06 | Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410384330.5A CN104140982A (en) | 2014-08-06 | 2014-08-06 | Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104140982A true CN104140982A (en) | 2014-11-12 |
Family
ID=51850323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410384330.5A Pending CN104140982A (en) | 2014-08-06 | 2014-08-06 | Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104140982A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123175A (en) * | 2015-07-22 | 2015-12-09 | 四川省农业科学院作物研究所 | Inoculation method for corn gray leaf spot |
| CN105255795A (en) * | 2015-12-01 | 2016-01-20 | 吉林省林业科学研究院 | Cytospora chrysosperma biocontrol bacterium |
| CN105420145B (en) * | 2015-11-12 | 2018-05-11 | 四川大学 | Strepiomyces lavendulae S3-5 and its application in grey leaf spot disease of maize is prevented |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533614A (en) * | 2012-02-21 | 2012-07-04 | 浙江大学 | Streptomyces strain and application thereof |
| CN103725640A (en) * | 2014-01-15 | 2014-04-16 | 东北林业大学 | Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof |
-
2014
- 2014-08-06 CN CN201410384330.5A patent/CN104140982A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533614A (en) * | 2012-02-21 | 2012-07-04 | 浙江大学 | Streptomyces strain and application thereof |
| CN103725640A (en) * | 2014-01-15 | 2014-04-16 | 东北林业大学 | Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 曾大鹏: "我国杨树病害的研究现状与防治", 《中国森林病虫》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123175A (en) * | 2015-07-22 | 2015-12-09 | 四川省农业科学院作物研究所 | Inoculation method for corn gray leaf spot |
| CN105123175B (en) * | 2015-07-22 | 2018-01-09 | 四川省农业科学院作物研究所 | A kind of inoculation method of grey leaf spot disease of maize |
| CN105420145B (en) * | 2015-11-12 | 2018-05-11 | 四川大学 | Strepiomyces lavendulae S3-5 and its application in grey leaf spot disease of maize is prevented |
| CN105255795A (en) * | 2015-12-01 | 2016-01-20 | 吉林省林业科学研究院 | Cytospora chrysosperma biocontrol bacterium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107446847B (en) | Bacillus belgii GT11 and application thereof | |
| CN105886428B (en) | One plant of Streptomycesalbidoflhaving and its application in microbial manure | |
| CN105219681B (en) | A kind of bacillus amyloliquefaciens Bacillus amyloliquefaciens D2WM and preparation method and application | |
| CN103509740B (en) | Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof | |
| CN105820981B (en) | The preparation and application of one plant height ground bacillus microbial inoculum | |
| CN105734000A (en) | Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases | |
| CN105670958A (en) | Low-temperature biocontrol bacterial strain and application thereof | |
| CN104877943A (en) | Antagonistic bacterium for controlling radix rehmannia root rot and application of antagonistic bacterium | |
| CN104480046A (en) | Brevibacillus laterosporus strain and application thereof | |
| CN105462881B (en) | A kind of Paenibacillus polymyxa and its application for being used to prevent crop verticillium wilt | |
| CN105543132A (en) | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases | |
| CN104651260A (en) | Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis | |
| CN103004886A (en) | Marine bacterium preparation for preventing banana vascular wilt | |
| CN102174431B (en) | Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon | |
| CN102172249A (en) | Marine bacteria 2BS12 preventing and controlling banana fusarium wilt | |
| CN106701619B (en) | high-density fermentation method of bacillus amyloliquefaciens and preparation method of microbial inoculum thereof | |
| CN104140982A (en) | Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs | |
| CN105018367A (en) | Bacillus marinus capable of inducing disease resistance and stress tolerance of plant | |
| CN106222121A (en) | A kind of bacillus megaterium bacterial strain, biocontrol agent and preparation method and application | |
| CN103951483B (en) | A kind of prevent and treat farm crop soil-borne disease biocontrol bacterial fertilizer and production technique and application | |
| CN102443559B (en) | Baclillus subtilis used for controlling cotton verticillium wilt and application thereof | |
| CN1327774C (en) | Tobacco rust resisting microbial prepn and its prepn process and application | |
| CN106148221A (en) | The stenotrophomonas antibacterial of one strain induction Semen arachidis hypogaeae root-knot nematode resistant | |
| CN100364411C (en) | Microbiological agent resisting rice blast, preparation method and application thereof | |
| CN104152372A (en) | Biocontrol strain G68 for preventing and treating plant diseases, and preparation method and application of microbial inoculant thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141112 |