CN104137809A - Feedstuff containing conjugated linoleic acid and used for improving sow production and piglet meat quality - Google Patents
Feedstuff containing conjugated linoleic acid and used for improving sow production and piglet meat quality Download PDFInfo
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- CN104137809A CN104137809A CN201410367799.8A CN201410367799A CN104137809A CN 104137809 A CN104137809 A CN 104137809A CN 201410367799 A CN201410367799 A CN 201410367799A CN 104137809 A CN104137809 A CN 104137809A
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Abstract
The invention relates to the field of feedstuff, in particular to a method for increasing the farrowing survival rate and the live piglet litter weight of sows through CLA feedstuff, reducing body weight loss of the sows after farrowing, impoving muscle fiber development of newly born piglets, and improving internal fat deposition of the newly born piglets.
Description
Technical field
The present invention relates to pig diet field, be specifically related to product that pig feed with CLA improves sow live young rate and the young litter weight of living, reduce the sow body weight loss in postpartum, newborn piglet body fat deposition is grown, improved to the muscle fibre that improves newborn piglet method.
Background technology
CLA is mainly present in ruminant product, sunflower oil and safflower oil, is the general name of a class 18 carbon polyunsaturated fatty acids that contain conjugated double bond (-C=C-C=C-).The conjugated double bond of CLA has 8,10,9,11,10,11,11,13 four kinds of position isomerisms; That every kind of position isomerism exists in theory is again suitable, along instead, anti-suitable, anti-four kinds of geometric isomers.Research in recent years shows, c9, and 11-CLA and t-10, the physiologically active such as that c12-CLA has is anticancer, anti arteriosclerosis, enhanced machine body immunity function, is added to and in pig diet, is also had the effect of improving meat.
Research is before Corino C for example, Pastorelli G, Douard V.et al.Conjugated linoleic acid in pig nutrition[J] .Productions animals, 2006,19 (1): 39-46 thinks, the piglet of taking in containing CLA breast milk has higher average daily gain and weanling weight, but CLA growth promoting function is not easy to be observed with fattening stage after wean.
Summary of the invention
Different from the instruction of prior art, the inventor is having following discovery after great many of experiments:
(1) CLA of diet interpolation suitable concentration can improve the product young rate alive of sow and the young litter weight of living, but the CLA of high concentration (2.0 % by weight) can affect the postnatal growth of piglet and survival; Add CLA simultaneously and can reduce the sow body weight loss in postpartum, make sow return to as early as possible normal level.
(2) Sow Diet is added the muscle fibre growth that CLA has affected newborn piglet, and main manifestations is for improving MyCH I type muscle fibre, and reduction MyHC 2x type muscle fibre; Slow switch fibers content showed increased, and fast muscle fiber content reduces.
(3) Sow Diet interpolation CLA has produced obvious impact to the fat deposition in newborn piglet body, has significantly reduced back fat weight, and has significantly improved dorsal muscles intramuscular fat content.
For above discovery, a kind of method that one of object of the present invention is to provide product that improves sow to live young rate and the young litter weight of living, described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration.
Two of object of the present invention is to provide a kind of method that reduces the body weight loss in sow postpartum, and described method comprises and gives the CLA that conceived and nursing sow is added with 0.5-2.5 % by weight concentration.
A kind of method that three of object of the present invention is to provide muscle fibre that improves newborn piglet to grow, described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration; Preferably, described improvement refers to and improves MyCH I type muscle fibre, and reduction MyHC 2x type muscle fibre.
Four of object of the present invention is to provide a kind of method of improving newborn piglet body fat deposition, and described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration; Preferably, described improvement refers to and has significantly reduced back fat weight, and significantly improved dorsal muscles intramuscular fat content.
Accompanying drawing explanation
Fig. 1 shows the changes in gene expression of piglet dorsal muscles muscle fiber types.* representative other ages in days under difference interpolation CLA condition are compared with 1d, significant difference (P<0.05); Different English alphabets represent same age in days, and each organizes significant difference (P<0.05).
Fig. 2 shows 28 age in days piglet dorsal muscles slow switch fibers and fast muscle fiber immunohistochemical analysis.A, B, C are slow switch fibers dyeing, and D, E, F are fast muscle fiber dyeing.
Fig. 3 shows the regulate gene expression variation that piglet dorsal muscles Growth of Muscle Fiber is grown.* representative other ages in days under difference interpolation CLA condition are compared with 1d, significant difference (P<0.05); Different English alphabets represent same age in days, and each organizes significant difference (P<0.05).
Fig. 4 shows piglet adipose tissue oil red O stain and observes (100 times of amplifications).A. control group; B.1.0%CLA group; C.2.0%CLA group.
Fig. 5 shows fat metabolism changes in gene expression in piglet dorsal muscles and back of the body fat.* representative other ages in days under difference interpolation CLA condition are compared with 1d, significant difference (P<0.05); Different English alphabets represent same age in days, and each organizes significant difference (P<0.05).
Embodiment
Explain in detail by the following examples the present invention, but described embodiment does not limit the scope of the invention.
Embodiment
Embodiment 1, the pregnancy duration and sow in lactation diet CLA content
Selection parity is close, 30 of the multiparity Rongchang Pig sows of pregnant 85d (with the artificial insemination of danish landrace boar seminal fluid), is divided at random 3 processed group: control group, 1%CLA interpolation group and 2%CLA interpolation group by breeding time and body weight.Every group of 10 repetitions, each repeats 1 pig.Sow every of pregnant 85d～phase of parturition day scale of feeding be 2.2kg, day feed 2 times; Divide the puerperium to change free choice feeding into, process constant.Piglet 10 ages in days are mended and to be opened foodstuff, 28 ages in days wean, and all piglets same of feeding is opened foodstuff, free choice feeding.Duration of test sow and offspring thereof all freely drink water, and feeding and management is carried out routinely.
CLA is purchased from Qingdao Australia marine growth Science and Technology Ltd., and purity is about 63.8%, c9 wherein, and t11-CLA and t10, c12-CLA content is respectively 29.0% and 30.4%.With reference to Rongchang Pig feeding standard (GB 7223-1987) preparation latter half of gestation and lactation basis diet, adjust and respectively process CLA and soybean oil addition, make control group containing CLA, other nutritive indexs are consistent.
The measured value of sow test diet CLA content is in Table 1.As shown in Table 1, the c9t11-CLA in 3 test diets and the content difference of t10c12-CLA are very obvious, increase and constantly increase, the object of Pass Test design with diet CLA addition.
The measured value (mg/g) of table 1 Sow Diet CLA content
Embodiment 2, diet add the impact of CLA on CLA content in sow milk and piglet serum
C9 in sow milk and piglet serum, t11-CLA and t10, c12-CLA content detection the results are shown in Table 2.As shown in Table 2, the pregnancy duration and lactation in diet, add CLA and significantly improved c9 in sow milk, t11-CLA and t10, c12-CLA content, and by lactation mode, significantly improved c9 in piglet body, t11-CLA and t10, c12-CLA content.C9 in the sow milk of two CLA interpolation groups, t11-CLA and t10, the c12-CLA content all utmost point is significantly higher than control group (P<0.01), and c9 in the piglet blood of two CLA interpolation groups, t11-CLA and t10, the c12-CLA content also utmost point is significantly higher than control group (P<0.01), and this result has been affirmed feasibility and the reliability of test.
CLA content (mg/g) in table 2 sow milk and piglet blood
Note: the different letter representations of going together are respectively organized significant difference (P<0.05).
Embodiment 3, diet add the impact of CLA on sow production performance
Diet adds CLA to sow production performance tool have a significant effect (in Table 3).Diet adds 1.0%～2.0%CLA and has significantly improved sow product young rate alive, compares with control group, has improved 5.90%～9.28% (P<0.05).Though young litter weight and the weanling pig survival rate of living average is without significant difference between each group, and 1.0% interpolation group has the trend higher than control group, has improved respectively 8.67% and 18.07%.It should be noted that 1.0% interpolation group does not go out life and death pig, and while weaning to 28d, death rate of this group piglet is minimum, survival rate is the highest; And the survival rate of 2.0% interpolation group piglet during to 28d is minimum in three groups.These data declarations, the CLA (1.0%) that Sow Diet is added suitable concentration processes product young rate alive and the young litter weight alive that can improve Rongchang County sow, but the CLA of high concentration (2.0%) can affect the postnatal growth of piglet and survival.
The impact of table 3 CLA on sow production performance
Note: the different letter representations of going together are respectively organized significant difference (P<0.05).
On the other hand, the pregnancy duration and lactation diet add CLA and can reduce the sow body weight loss in postpartum (in Table 3), with control group ratio, add 1.0% and 2.0%CLA make respectively the body weight loss in 14 days postpartum of sow reduce 11.92% and 26.08%; And 2.0%CLA makes the body weight loss in 28 days postpartum of sow reduce by 20.98% (P<0.05).With control group ratio, within 14 days postpartum, the feed consumption rate of CLA processed group sow has reduced respectively 10.02% and 20.31%, but 28 days postpartum, the feed consumption rate of three groups was almost as broad as long.These data declarations, CLA processes can reduce Rongchang County sow body weight loss in postpartum, makes sow return to as early as possible normal level.
Embodiment 4, diet add the impact of CLA on newborn piglet growth performance
In the pregnancy duration and sow in lactation diet, add CLA the impact of newborn piglet growth performance be the results are shown in Table to 3-5.As seen from table, at 1,7,14 and 28 ages in days, though each organizes piglet average weight difference not remarkable (P>0.05), 1.0% interpolation group is a little more than other two groups.From testing the full phase, it is not remarkable that each organizes piglet Average weight increasing a day difference, but numerically, 1.0% interpolation group is greater than other two groups, and 2.0% interpolation group and control group almost do not have difference (P>0.05).Above results suggest, adds the growth that 1%CLA can promote newborn piglet to a certain extent in daily ration in sow gestation phase and lactation.
The impact of table 4 CLA on newborn piglet growth performance
Note: the different letter representations of going together are respectively organized significant difference (P<0.05).
Embodiment 5, diet add the impact that CLA grows newborn piglet muscle fibre
In the pregnancy duration and sow in lactation diet, add CLA the impact of newborn piglet longissimus dorsi muscle diameter of muscle fiber and density be the results are shown in Table to 5.As seen from table, piglet diameter of muscle fiber and density are subject to the impact remarkable (P<0.0001) of age in days, with piglet age in days, increase, and the continuous chap of diameter of muscle fiber, muscle fibre density constantly reduces.Though piglet diameter of muscle fiber and density are not subject to the impact (P>0.2005) that CLA processes and age in days * CLA does mutually, but for 14～28 age in days piglets, Sow Diet is added CLA the trend that reduces diameter of muscle fiber and increase muscle fibre density.This is more consistent with the result that CLA in growing-finishing pig test improves meat quality.
The impact of table 5 CLA on piglet diameter of muscle fiber and density
The gene expression of four kinds of muscle fiber typeses of piglet dorsal muscles (MyHCI, 2a, 2x and 2b type) of 1,7,14 and 28 ages in days that adopted quantitative PCR analysis, the results are shown in Figure 1.
As shown in Figure 1, the myofibrillar expression of MyCH I type increases and improves constantly (P<0.05) with piglet age in days, and except 1 age in days, other ages in days process with CLA the myofibrillar expression of MyCH I type (P<0.05) that has been significantly increased.MyHC 2a and the myofibrillar expression of 2x type also increase with piglet growth, and at 14 and 28 ages in days, reach significance level (P<0.05), but CLA processing 14 age in days MyHC2a type muscle fibres and 7～28 myofibrillar expression of age in days MyHC 2x type (P<0.05) have significantly been reduced.The myofibrillar expression of MyCH 2b type slightly increases with piglet growth, but difference is significantly (P>0.05) not, and diet add CLA on the type muscle fibre because of the impact expressed also not obvious (P>0.05).
Adopt Immunohistochemical Method to analyze 28 age in days piglet dorsal muscles fast and slow muscle fibre content, the results are shown in Figure 2.As seen from the figure, with control group ratio, the piglet dorsal muscles slow switch fibers content showed increased of CLA processed group, and fast muscle fiber content reduces.This is consistent with MyHC I, 2a, 2x and the myofibrillar result of 2b type of employing quantitative PCR analysis.Because the increase of slow switch fibers content contributes to improve meat quality, this has also proved the result that CLA can improve meat quality.
Test adopts quantitative PCR analysis further to analyze the functional gene relevant to muscle growth and expresses, and the results are shown in Figure 3.As seen from the figure, myocyte's growth factor MyoG mRNA level occurs significantly raising (P<0.05) piglet postnatal early stage (1～7 age in days), changes subsequently not obvious; And except 28 ages in days, add CLA and improved MyoG mRNA level, wherein at 7 and 14 ages in days, reach significance level (P<0.05).Myocyte breaks up regulatory factor MyoD mRNA level to be increased with piglet growth, and 7,14 and 28 ages in days are all significantly higher than 1 age in days (P<0.05); And at 28 ages in days, CLA processes the expression (P<0.05) that significantly increases this gene.Myostatin MSTN mRNA level increases with piglet age in days and significantly increases (P<0.05), but CLA processing has obviously reduced this gene expression of 1～28 age in days (P<0.05).These results demonstrations, CLA processes and can promote the early stage muscle fibre of piglet growth to grow, and slow switch fibers is increased, and is conducive to the improvement of meat.
Embodiment 6, diet add the impact of CLA on piglet body fat deposition
In the pregnancy duration and sow in lactation diet, add CLA the fat deposition in piglet body has been produced to obvious impact (in Table 6).During 28 age in days, piglet stomach fat weight is at each group difference not significantly (P>0.05), but the back fat weight of two CLA processed group is all significantly lower than control group (P<0.05), and muscle of back intramuscular fat content is all apparently higher than control group (P<0.05).The piglet intramuscular fat content of CLA processed group is all apparently higher than control group (P<0.05).
The impact of table 6 CLA on piglet growth performance and body lipidosis
Note: the different letter representations of going together are respectively organized significant difference (P<0.05).
Get 28 age in days piglet neck fat tissues and carry out frozen section and oil red O stain.Micro-Microscopic observation discovery, each is organized adipocyte and is all round or ellipse, be full of fat and drip, but the adipocyte diameter of CLA processed group will be significantly less than control group (Fig. 4) in born of the same parents.
The fat metabolism regulate gene expression that test adopts quantitative PCR technique further to analyze in piglet back fat and muscle changes.Wherein, synthetase of fatty acid (Fatty acid synthetase, FAS) be the synthetic main rate-limiting enzyme of body fat acid, it is one of most important factor affecting animal tallow deposition, and lard type triglyceride hydrolysis enzyme (Adipose triglyceride lipase, ATGL) be the main rate-limiting enzyme of triglyceride hydrolysis, lipolysis is had to extremely important effect.Result of the test is found, all constantly risings with piglet growth of the mRNA level of FAS and ATGL in back of the body fat and dorsal muscles, and wherein 28 ages in days are significantly higher than 1 age in days (P<0.05 is shown in Fig. 5).During 1 age in days, the expression of FAS and ATGL no significant difference (P>0.05 is shown in Fig. 3-5) all in the dorsal muscles of three groups of piglets and back of the body fat.During 14 age in days, in the back of the body fat of 2.0%CLA interpolation group, ATGL mRNA level is significantly higher than other two groups (P<0.05 is shown in Fig. 5 D), and the equal remarkable (P>0.05 of the differential expression of three groups of dorsal muscles FAS and ATGL, see Fig. 5 A, D).During 28 age in days, the dorsal muscles FAS mRNA level of two CLA interpolation groups is all significantly higher than control group (P<0.05 is shown in Fig. 5 A), and ATGL mRNA level is all a little less than control group (P>0.05 is shown in Fig. 5 C); But the back of the body fat FAS mRNA level of two CLA interpolation groups is all significantly lower than control group (P<0.05 is shown in Fig. 5 B), and ATGL mRNA level is all significantly higher than control group (P<0.05 is shown in Fig. 5 D).These results have further been explained above-mentioned apparent result, and CLA reduces piglet back fat deposition and increases intramuscular fat content.
Claims (10)
1. the product that the improves sow method for young rate and the young litter weight of living of living, described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration.
2. according to the method for claim 1, described method comprises and gives the CLA that farrowing sow is added with 1.0-2.0 % by weight concentration.
3. reduce a method for the body weight loss in sow postpartum, described method comprises and gives the CLA that conceived and nursing sow is added with 0.5-2.5 % by weight concentration.
4. according to the method for claim 3, described method comprises and gives the CLA that farrowing sow is added with 1.0-2.0 % by weight concentration.
5. a kind of method that provides muscle fibre that improves newborn piglet to grow, described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration.
6. according to the method for claim 5, described method comprises and gives the CLA that farrowing sow is added with 1.0-2.0 % by weight concentration.
7. according to the method for claim 5 or 6, described improvement refers to and improves MyCH I type muscle fibre, and reduction MyHC 2x type muscle fibre.
8. improve a method for newborn piglet body fat deposition, described method comprises and gives the CLA that farrowing sow is added with 0.5-2.5 % by weight concentration.
9. method according to Claim 8, described method comprises and gives the CLA that farrowing sow is added with 1.0-2.0 % by weight concentration.
10. according to Claim 8 or 9 method, described improvement refers to and has significantly reduced back fat weight, and significantly improved dorsal muscles intramuscular fat content.
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Cited By (1)
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| CN108935321A (en) * | 2018-08-02 | 2018-12-07 | 四川科为民生物技术有限公司 | One kind being rich in CLA nutrition swine rearing method |
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Application publication date: 20141112 |