CN104106481B - A kind of method of large yellow Crocker enhancement releasing mark - Google Patents
A kind of method of large yellow Crocker enhancement releasing mark Download PDFInfo
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- CN104106481B CN104106481B CN201410283245.XA CN201410283245A CN104106481B CN 104106481 B CN104106481 B CN 104106481B CN 201410283245 A CN201410283245 A CN 201410283245A CN 104106481 B CN104106481 B CN 104106481B
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- strontium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Farming Of Fish And Shellfish (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention relates to a kind of method of large yellow Crocker enhancement releasing mark, comprise: in the nursery pond of large yellow Crocker of releasing, add Strontium dichloride hexahydrate, improve water body strontium ion concentration to 0.05 ~ 0.10g/L in nursery pond, strontium mark is carried out to the prelarva that age in days is 30 ~ 40 days, the mark time is 10 days, and mark terminates to carry out strontium element content detection to otolith abrasive disc afterwards.Present invention process is simple, and have stability and convenience, the enhancement effect evaluation that can be large yellow Crocker provides effective means, and can be used for long-term follow and monitor its effect of releasing, for fisheries management, department provides scientific basis.
Description
Technical field
The invention belongs to mark field of releasing, particularly a kind of method of large yellow Crocker enhancement releasing mark.
Background technology
Marker technique is the important tool that researching fish enhancement effect is evaluated, conventional denotation approach has externally to list, cuts fin ray and fluorescence etc., but these methods cause certain injury or less stable to fish cognition, and need a large amount of material resources and manpower, and are not suitable for the mark of extensive seed.In order to better services is in enhancement releasing work, adopt strontium ion (Sr
2+) mark Fish otolith method, solve a mark difficult problem for extensive seed.In ocean, strontium ion concentration is comparatively stable, is about 7.6 ~ 8.1mg/L, and strontium and calcium (Ca) belong to congeners, are deposited in Fish otolith, to fish without any nocuity with the replaceable calcium constituent of strontium element.Relative traditional labeling methods, strontium ion notation can mark large-scale fry, facility simple to operate; Again there is stability, seed long-term follow investigation of being convenient to release, be beneficial to Scientific evaluation increment and release benefit.
One of economic fish that large yellow Crocker Zeng Shi China is important, due to reasons such as overfishings, the resource of large yellow Crocker is in depletion.In order to recover the resource of large yellow Crocker, it is movable to have carried out large-scale enhancement releasing, but due to the long distance migration characteristic of large yellow Crocker and the restriction of denotation approach, fails Efficient Evaluation its effect of releasing.Given this, the effective follow-up investigation of a kind of new denotation approach is needed to release the survival state of seed, with Scientific evaluation enhancement effect.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method of large yellow Crocker enhancement releasing mark, the method technique is simple, there is stability and convenience, the enhancement effect evaluation that can be large yellow Crocker provides effective means, can be used for long-term follow and monitor its effect of releasing, for fisheries management, department provides scientific basis.
The method of a kind of large yellow Crocker enhancement releasing mark of the present invention, comprising:
Strontium dichloride hexahydrate is added in the nursery pond of large yellow Crocker of releasing, improve water body strontium ion concentration to 0.05 ~ 0.10g/L in nursery pond, carry out strontium mark to the prelarva that age in days is 30 ~ 40 days, the mark time is 10 days, and mark terminates to carry out strontium element content detection to otolith abrasive disc afterwards.
Add the Strontium dichloride hexahydrate of equivalent after changing water in described seedling raising process at every turn.
Described strontium element content detection adopts Inductively coupled plasma mass spectrometry.
On the pseudosciaena crocea fish otolith of strontium mark there is Sr/Ca ratio crest in distance otolith core 0.7 ~ 0.9mm place.
1. large yellow Crocker nursery pond Strontium in Sea Water ion concentration range is between 0.05 ~ 0.10g/L, and required for add Strontium dichloride hexahydrate (SrCl in nursery pond
26H
2o) weight, can according to formula M=C*V*266.62 (SrCl
26H
2o molecular weight)/87.62 (Sr molecular weight) ≈ 3.04C*V, wherein M is the weight needing to add Strontium dichloride hexahydrate, and C is nursery pond Strontium in Sea Water ion concentration (0.05 ~ 0.10g/L), and V is water body volume in nursery pond.Corresponding content of strontium rising in pseudosciaena crocea fish otolith, form crest, its Sr/Ca ratio range between 13.29 ~ 24.63mmol/mol, and does not indicate that in individuality, Sr/Ca average of relatives is 2.42mmol/mol.
2. tag time is 10 days, and the foster temporarily of high concentration strontium seawater of 10 days can form the Sr/Ca ratio crest being about 0.2mm width on the otolith of large yellow Crocker prelarva, differentiates for judging.Time of supporting temporarily is too short, and Sr/Ca ratio crest is narrower and not easy to identify, long, increases cost.
3. to be age in days be time of starting of the mark large yellow Crocker prelarva (after hatching 30 ~ 40 days) of 30 ~ 40 days.Due to large yellow Crocker seed in nursery pond grow to 50 ages in days, body be about as 20mm time, need be transferred in open waters net cage and support temporarily, otherwise can be too small because of water body volume, affect seed growth.Therefore in order to coordinate nursery work and seed growth needs, the mark last applicable time shifts net cage first 10 days in seed, and namely age in days is the prelarva of 40 days.And the prelarva after hatching in theory can indicate, but its peak forming Sr/Ca ratio is close to otolith core, during grinding otolith easily by this region mill thoroughly, cause experimental error, add experiment abrasive disc difficulty, therefore select age in days be 30 days prelarva for mark the time started ideal.
4. according to mark concentration and mark time started, at least 4 prescription cases can being combined, for indicating that difference is released the seed of place and different time of releasing, effective means can be provided for further investigation enhancement effect.4 prescription cases can be divided into the combinations such as 0.05g/L strontium ion concentration and age in days 30 days, 0.05 strontium ion concentration and age in days 40 days, 0.10g/L strontium ion concentration and age in days 30 days and 0.10 strontium ion concentration and age in days 40 days.Method of discrimination between various combination be 0.05g/L strontium ion concentration symbols seed otolith on Sr/Ca average of relatives be 13.29mmol/mol, and the Sr/Ca average of relatives on the seed otolith of 0.10g/L strontium ion concentration symbols is 24.63mmol/mol; Be 40 days prelarvas compared to age in days, age in days is distance otolith core nearly about 0.2mm in Sr/Ca ratio peak on the prelarva otolith of 30 days.
beneficial effect
Present invention process is simple, and have stability and convenience, the enhancement effect evaluation that can be large yellow Crocker provides effective means, and can be used for long-term follow and monitor its effect of releasing, for fisheries management, department provides scientific basis.
Accompanying drawing explanation
Fig. 1 is the otolith Sr/Ca ratio schematic diagram of large yellow Crocker mark prelarva;
Fig. 2 is recapture large yellow Crocker otolith Sr/Ca ratio schematic diagram.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
1. indicate the stage: release in the nursery of large yellow Crocker, by adding Strontium dichloride hexahydrate, improving water body strontium ion concentration in nursery pond and, to 0.05 ~ 0.10g/L, strontium mark being carried out to the prelarva that age in days is 30 ~ 40 days.Rearing conditions, all in the normal habitat of nursery defined, only improves and raises strontium ion concentration in water body.Owing to needing change water every day in seedling raising process, need the Strontium dichloride hexahydrate adding equivalent after therefore changing water, concrete weight is by formula and change water volume and calculate and learn, to guarantee the stable of strontium ion concentration in nursery pond at every turn.The strontium flag tag time is 10 days.After mark terminates, stay 10 tail large yellow Crocker prelarvas as sample, to compare.
2. the processing stage of check sample: check sample is measured body and grown and take otolith, and use the grinding of otolith grinder to make otolith abrasive disc.Guarantee by otolith core in process of lapping, ensure the accuracy of data.After this, utilize Inductively coupled plasma mass spectrometry (ICP-MS) otolith abrasive disc to be carried out to the detection of strontium element content, its result as shown in Figure 1.On the pseudosciaena crocea fish otolith of strontium mark as can be seen from Figure there is Sr/Ca ratio crest in distance otolith core 0.7 ~ 0.9mm place.
3. recapture and detection-phase: large yellow Crocker seed is a large amount of after releasing obtains large yellow Crocker sample, and whether carry out conventional biology measurement, take otolith and make detectable otolith abrasive disc, detect Sr/Ca ratio by ICP-MS, be individuality of releasing for detecting.Fig. 2 is the Sr/Ca ratio schematic diagram of recapture large yellow Crocker otolith, and as in detection sample, at distance otolith core 0.7 ~ 0.9mm place, occur Sr/Ca ratio peak, then this sample is the individuality of tagging.
Claims (3)
1. a method for large yellow Crocker enhancement releasing mark, comprising:
Strontium dichloride hexahydrate is added in the nursery pond of large yellow Crocker of releasing, improve water body strontium ion concentration to 0.05 ~ 0.10g/L in nursery pond, carry out strontium mark to the prelarva that age in days is 30 ~ 40 days, the mark time is 10 days, and mark terminates to carry out strontium element content detection to otolith abrasive disc afterwards; Wherein, the Strontium dichloride hexahydrate SrCl in nursery pond is added required for
26H
2the weight of O, calculate according to formula M=C*V*266.62/87.62 ≈ 3.04C*V, wherein M is the weight needing to add Strontium dichloride hexahydrate, and C is nursery pond Strontium in Sea Water ion concentration, and V is water body volume in nursery pond.
2. the method for a kind of large yellow Crocker enhancement releasing mark according to claim 1, is characterized in that: described strontium element content detection adopts Inductively coupled plasma mass spectrometry.
3. the method for a kind of large yellow Crocker enhancement releasing mark according to claim 1, is characterized in that: on the pseudosciaena crocea fish otolith of strontium mark, Sr/Ca ratio crest appears in distance otolith core 0.7 ~ 0.9mm place.
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| CN201410283245.XA CN104106481B (en) | 2014-06-23 | 2014-06-23 | A kind of method of large yellow Crocker enhancement releasing mark |
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| CN201410283245.XA CN104106481B (en) | 2014-06-23 | 2014-06-23 | A kind of method of large yellow Crocker enhancement releasing mark |
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| CN104106481B true CN104106481B (en) | 2016-03-02 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105684971A (en) * | 2016-02-24 | 2016-06-22 | 中国科学院水生生物研究所 | Method for distinguishing artificially-bred Gymnocypris przewalskii and naturally-bred Gymnocypris przewalskii and application |
| CN106172109B (en) * | 2016-07-13 | 2019-01-01 | 海阳市黄海水产有限公司 | Flounder flounder individual separator method |
| CN107258647B (en) * | 2017-05-24 | 2019-11-08 | 中国水产科学研究院黄海水产研究所 | A kind of lefteye flounder fish otolith rubidium marking method |
| CN110463644A (en) * | 2019-08-20 | 2019-11-19 | 中国水产科学研究院东海水产研究所 | A kind of otolith rubidium marking method of black porgy enhancement releasing fry |
| CN113552153A (en) * | 2021-07-27 | 2021-10-26 | 中国水产科学研究院淡水渔业研究中心 | Strontium finlet marking method for non-lethal freshwater fish and detection method thereof |
| CN116307893B (en) * | 2023-03-20 | 2024-01-30 | 广东海洋大学 | Fish proliferation releasing water area suitability evaluation system based on data analysis |
Citations (3)
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| CN102273418A (en) * | 2011-06-24 | 2011-12-14 | 中国水产科学研究院珠江水产研究所 | Fluorescent labeling method of fish otolith |
| CN102960275A (en) * | 2012-11-20 | 2013-03-13 | 中国长江三峡集团公司 | Method for marking grass carp otolith by fluorescent substances |
| CN102960274A (en) * | 2012-11-20 | 2013-03-13 | 中国长江三峡集团公司 | Method for marking fish otolith by fluorescent substances |
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| JP2001275512A (en) * | 2000-04-03 | 2001-10-09 | Sumitomo Metal Mining Co Ltd | Method of identifying natural sweetfish and cultivated sweetfish |
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Patent Citations (3)
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|---|---|---|---|---|
| CN102273418A (en) * | 2011-06-24 | 2011-12-14 | 中国水产科学研究院珠江水产研究所 | Fluorescent labeling method of fish otolith |
| CN102960275A (en) * | 2012-11-20 | 2013-03-13 | 中国长江三峡集团公司 | Method for marking grass carp otolith by fluorescent substances |
| CN102960274A (en) * | 2012-11-20 | 2013-03-13 | 中国长江三峡集团公司 | Method for marking fish otolith by fluorescent substances |
Non-Patent Citations (2)
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| 环境因子对大黄鱼耳石锶元素富集的影响;张翼等;《海洋渔业》;20130815;第35卷(第3期);第278-288页 * |
| 鱼类的耳石信息分析及生活史重建――理论、方法与应用;窦硕增;《海洋科学集刊》;20070515(第00期);第93-112页 * |
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