CN104094924A - Cryoprotectant for human sperm - Google Patents
Cryoprotectant for human sperm Download PDFInfo
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- CN104094924A CN104094924A CN201310172542.2A CN201310172542A CN104094924A CN 104094924 A CN104094924 A CN 104094924A CN 201310172542 A CN201310172542 A CN 201310172542A CN 104094924 A CN104094924 A CN 104094924A
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Abstract
The invention discloses a cryoprotectant for a human sperm. The cryoprotectant comprises aseptic deionized water, fresh yolk, glycerin, sodium citrate, glucose, fructose, caffeine and cyclodextrin, wherein a volume ratio of deionized water to fresh yolk to glycerin is 5: 1: 1, and a mass ratio of fresh yolk to glycerin to sodium citrate to glucose to fructose to caffeine to cyclodextrin is 0.2 mg/ml: 0.15 mg/ml: 13 mg/ml: 10 mg/ml: 5 mg/ml: 1 mg/ml: 1 mg/ml. A preparation method for the cryoprotectant comprises the following steps: filling a container with 100 ml of aseptic deionized water; then successively adding 1.22 g of sodium citrate, 0.78 g of glucose, 0.68 g of fructose, 20 ml of fresh yolk, 20 ml of glycerin, 0.5 g of caffeine and 0.5 g of cyclodextrin; and finally, uniformly mixing all the above-mentioned raw materials, putting the obtained mixture in a water bath with a temperature of 56 DEG C for 30 min for inactivation, carrying out cooling, then carrying out filtering and split charging and standing the obtained cryoprotectant for subsequent usage. According to the invention, the sperm can be better protected through a novel formula; on the basis of conventional cryoprotectants for the human sperm, the post-thawing and recovery rate of the human sperm is increased, and the original functions of the sperm are better maintained.
Description
Technical field
The present invention relates to technical field of bioengineering, particularly a kind of human sperm's cryoprotector.
Background technology
Human sperm's freezing preservation has positive effect for human reproduction's health, and one of key factor that affects controlled-rate freezing is the cryoprotector adding in refrigerating process.
At human sperm bank, human sperm's freezing preservation has had very ripe reagent and technology, and for the preservation of other cells, sperm storage more must be preserved its energy and fertility when keeping its activity ratio.
The chief component of domestic conventional cryoprotector is yolk and glycerine at present, and external commercialization cryoprotector chief component is albumin and glycerine.In general the ratio of yolk and glycerine is 10%-15%, and the ratio of yolk is 15%-20%, uses these two kinds of cryoprotectors can make the Cryopreservation rate of sperm be stabilized in 60%-70%, can reach the requirement of the Ministry of Public Health to sperm bank.But for each reproductive center, auxiliary procreation technology needs the sperm that energy is better, function is more complete.
Summary of the invention
The defect existing in order to overcome above-mentioned prior art, the invention provides a kind of sperm freezing protecting agent, by new formula, better protects sperm, on existing human sperm's cryoprotector basis, has improved Cryopreservation rate, keeps better sperm original function.
To achieve these goals, technical scheme of the present invention is as follows:
A kind of human sperm's cryoprotector; comprise that volume ratio is the aseptic ionized water of 5: 1: 1, fresh yolk and glycerine; also comprise sodium citrate, glucose, fructose, caffeine and cyclodextrin; its quality proportion relation is:: fresh yolk 0.2mg/ml; glycerine 0.15mg/ml, sodium citrate 13mg/ml, glucose 10mg/ml, fructose 5mg/ml, caffeine 1mg/ml and cyclodextrin 1mg/ml.。
Its collocation method is as described below:
First, with the aseptic ionized water of container splendid attire 100ml.
Then, successively add successively 1.22g sodium citrate, 0.78g glucose, 0.68g fructose, the fresh yolk of 20ml, 20ml glycerine, 0.5g caffeine and 0.5g cyclodextrin.
Final step is, after above-mentioned each raw material is mixed, puts the deactivation in 30 minutes of 56 ℃ of water baths, after cooling, filters packing finished product, stand-by.
The invention has the beneficial effects as follows; before comparing, sperm freezing protecting agent is a kind of brand-new formula; method is novel; on existing human sperm's cryoprotector basis; improved Cryopreservation rate; keep better sperm original function, can provide the sperm that energy is better, function is more complete for reproductive center.
Embodiment:
In order to make technological means of the present invention, creation characteristic and to reach object, be easy to understand understanding, below in conjunction with specific embodiment, further set forth the present invention.
Embodiment:
A kind of sperm freezing protecting agent of the present invention; comprise that volume ratio is the aseptic ionized water of 5: 1: 1, fresh yolk and glycerine; also comprise sodium citrate, glucose, fructose, caffeine and cyclodextrin; its quality proportion relation is:: fresh yolk 0.2mg/ml; glycerine 0.15mg/ml, sodium citrate 13mg/ml, glucose 10mg/ml, fructose 5mg/ml, caffeine 1mg/ml and cyclodextrin 1mg/ml.。
Its collocation method is as described below:
First, with the aseptic ionized water of container splendid attire 100ml.
Then, successively add successively 1.22g sodium citrate, 0.78g glucose, 0.68g fructose, the fresh yolk of 20ml, 20ml glycerine, 0.5g caffeine and 0.5g cyclodextrin.
Final step is, after above-mentioned each raw material is mixed, puts the deactivation in 30 minutes of 56 ℃ of water baths, after cooling, filters packing finished product, stand-by.
The effect of above-mentioned each constitutive material composition is: add 1.22g sodium citrate, for maintaining protectant acid base equilibrium; Add 0.78g glucose, for spermoblast film, have protective effect; Adding 0.68g fructose, is the nutriment of sperm; Add the fresh yolk of 20ml, protection perforatorium, improves sperm motility rate.
Add 20ml glycerine, permeable mass, can infiltrate in sperm membrane, the infringement that the minimizing ice crystal phase causes sperm; Add 0.5g caffeine, can significantly improve vigor and the rate in vitro fertilization of sperm; Add 0.5g cyclodextrin, anti-oxidant, protection plasmalemmae of sperms and acrosomal integnity.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. human sperm's cryoprotector; it is characterized in that; comprise that volume ratio is the aseptic ionized water of 5: 1: 1, fresh yolk and glycerine; also comprise sodium citrate, glucose, fructose, caffeine and cyclodextrin; its quality proportion relation is:: fresh yolk 0.2mg/ml; glycerine 0.15mg/ml, sodium citrate 13mg/ml, glucose 10mg/ml, fructose 5mg/ml, caffeine 1mg/ml and cyclodextrin 1mg/ml.。
2. a kind of human sperm's cryoprotector according to claim 1, is characterized in that, its collocation method is as described below:
(1) first, with the aseptic ionized water of container splendid attire 100ml;
(2) then, successively add successively 1.22g sodium citrate, 0.78g glucose, 0.68g fructose, the fresh yolk of 20ml, 20ml glycerine, 0.5g caffeine and 0.5g cyclodextrin.
(3) final step is, after above-mentioned each raw material is mixed, puts the deactivation in 30 minutes of 56 ℃ of water baths, after cooling, filters packing finished product, stand-by.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310172542.2A CN104094924A (en) | 2013-04-13 | 2013-04-13 | Cryoprotectant for human sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310172542.2A CN104094924A (en) | 2013-04-13 | 2013-04-13 | Cryoprotectant for human sperm |
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| CN104094924A true CN104094924A (en) | 2014-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310172542.2A Pending CN104094924A (en) | 2013-04-13 | 2013-04-13 | Cryoprotectant for human sperm |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365583A (en) * | 2014-11-07 | 2015-02-25 | 郜鸿生物科技（上海）有限公司 | Extremely-low sperm freezing protective agent and application thereof |
| CN108651440A (en) * | 2018-04-18 | 2018-10-16 | 中南大学 | A kind of supper-fast freezen protective system of human seminal fluid |
| EP3284795A4 (en) * | 2015-04-16 | 2019-03-20 | The School Corporation Kansai University | Anti-ice nucleation activator |
| KR102364057B1 (en) * | 2021-01-26 | 2022-02-17 | 한국식품연구원 | Method for microorganism cryoprotectant using coffee residue |
| KR102463256B1 (en) * | 2022-05-02 | 2022-11-07 | 주식회사 프롬바이오 | Composition for Low-temperatuer Preservation or Cryopreservation of Fat Tissue Using Caffeine, etc. and the Preservation Method of Fat Tissue Using the Same |
-
2013
- 2013-04-13 CN CN201310172542.2A patent/CN104094924A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365583A (en) * | 2014-11-07 | 2015-02-25 | 郜鸿生物科技（上海）有限公司 | Extremely-low sperm freezing protective agent and application thereof |
| CN104365583B (en) * | 2014-11-07 | 2016-08-24 | 郜鸿生物科技（上海）有限公司 | A kind of rare sperm freezing protecting agent and application thereof |
| EP3284795A4 (en) * | 2015-04-16 | 2019-03-20 | The School Corporation Kansai University | Anti-ice nucleation activator |
| US10633570B2 (en) | 2015-04-16 | 2020-04-28 | The School Corporation Kansai University | Anti-ice nucleation activator |
| CN108651440A (en) * | 2018-04-18 | 2018-10-16 | 中南大学 | A kind of supper-fast freezen protective system of human seminal fluid |
| KR102364057B1 (en) * | 2021-01-26 | 2022-02-17 | 한국식품연구원 | Method for microorganism cryoprotectant using coffee residue |
| KR102463256B1 (en) * | 2022-05-02 | 2022-11-07 | 주식회사 프롬바이오 | Composition for Low-temperatuer Preservation or Cryopreservation of Fat Tissue Using Caffeine, etc. and the Preservation Method of Fat Tissue Using the Same |
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Application publication date: 20141015 |