CN104094832A - Method for crossbreeding of undaria pinnatifida by using unisexual planospores - Google Patents
Method for crossbreeding of undaria pinnatifida by using unisexual planospores Download PDFInfo
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- CN104094832A CN104094832A CN201410305405.6A CN201410305405A CN104094832A CN 104094832 A CN104094832 A CN 104094832A CN 201410305405 A CN201410305405 A CN 201410305405A CN 104094832 A CN104094832 A CN 104094832A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 14
- 241001261506 Undaria pinnatifida Species 0.000 title abstract 3
- 239000013535 sea water Substances 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 230000000366 juvenile effect Effects 0.000 claims description 19
- 241001261505 Undaria Species 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 12
- 230000035800 maturation Effects 0.000 claims description 12
- 239000002184 metal Substances 0.000 claims description 12
- 229910052751 metal Inorganic materials 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 9
- 230000008186 parthenogenesis Effects 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 208000027877 Disorders of Sex Development Diseases 0.000 claims description 6
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 6
- 230000004720 fertilization Effects 0.000 claims description 6
- 201000005611 hermaphroditism Diseases 0.000 claims description 6
- 230000010196 hermaphroditism Effects 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 208000013327 true hermaphroditism Diseases 0.000 claims description 6
- 230000009182 swimming Effects 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000006698 induction Effects 0.000 abstract description 2
- 230000001568 sexual effect Effects 0.000 abstract description 2
- 238000010899 nucleation Methods 0.000 abstract 2
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000028070 sporulation Effects 0.000 description 2
- 241001260874 Sargassum horneri Species 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Cultivation Of Plants (AREA)
- Farming Of Fish And Shellfish (AREA)
- Cultivation Of Seaweed (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for crossbreeding of undaria pinnatifida, in particular to a method for crossbreeding of the undaria pinnatifida by using unisexual planospores. Female gametophytes are subjected to parthenogernesis induction to generate sporophores, meanwhile, hermaphroditic gametophytes are subjected to development cultivation to generate selfing sporophores, when the sporophores are longer than 1mm, the sporophores are changed into inflating cultivation conditions and cultivated to sexual maturity. Mature sporophyls are placed in sea water for releasing the planospores, the planospores are attached on a seeding curtain after the color of sea water turns into tawny, and the seeding curtain is then placed in fresh sea water. The planospores released by the sporophores generated by parthenogernesis are female planospores, and the planospores released by the hermaphroditic gametophytes are male planospores. According to the method, the male and female gametophyte hybridization technique is improved by the male and female planospore hybridization technique, and the method has great significance in applying monoclonal crossbreeding to the production practice in large scales.
Description
Technical field
The present invention relates to the method for undaria pinnitafida crossbreeding, specifically a kind of method of utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding.
Background technology
Undaria pinnitafida seedling fostering has trip spore to collect seedling and the gametophyte two kinds of approach of collecting seedling, grow seedlings on a large scale at present produce in taking the former as main, the latter only helps out.The advantage of gametophyte clone crossbreeding is to produce the consistent sporophyte offspring of proterties height, and traditional trip spore breeding there will be the differentiation of sporophyte characters of progenies.But the major defect that gametophyte is collected seedling is, after gametophyte clone is inoculated into seedling curtain, to be just attached to Miao Shengshang, the non-constant of its firmness by the stickum of frond secretion.And juvenile sporophyte is before rhizoid forms, and relies on egagametophyte and is attached to Miao Shengshang.Therefore when, this loose adhesive strength is at sea supported after causing and emerging temporarily, de-growth of cereal crop seedlings condition is very serious.By contrast, trip spore is much higher to the adhesive strength of matrix.In addition, adopt gametophyte to grow seedlings, quantitatively cannot the short time meet Production requirement, need to expend a large amount of time, manpower and financial resources carries out gametophytic cultivation amplification, and trip spores release amount is large, does not have this drawback.Therefore, adopt the female trip spore of single traits can obtain with male trip spore hybridization the sporophyte offspring that proterties is consistent, can solve again gametophyte clone seedling cost high, spatfall rate is low, the de-serious problem of seedling, will grow seedlings on a large scale to produce to undaria pinnitafida produces positive impetus.
Summary of the invention
The object of the invention is to provide a kind of method of utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding.
The technical solution used in the present invention is for achieving the above object:
A method of utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding,
1) female gamete body is induced and be cultured to juvenile sporophyte and reach 1mm and proceed to above under inflation condition of culture, the history of life that completes parthenosporophyte, form the sporophyte of parthenogenesis maturation;
2) hermaphroditic gametophyte is carried out to Development culture, produce selfing sporophyte, grow to 1mm when above until sporophyte, proceed to and inflate under condition of culture, the history of life that completes selfing sporophyte, form ripe selfing sporophyte;
3) sporophyll of above-mentioned two kinds of ripe sporophytes is placed in to seawater release and obtains else trip spore of two kinds of unicity, after discharging the color intensity of sea water that obtains swimming spore and being yellowish-brown, two kinds of trip spore fluids are mixed and are attached on seedling curtain, then seedling curtain is put into fresh seawater;
4) the above-mentioned seedling curtain of swimming spore that adheres to is at light intensity 50-80 μ mol photons m
-2s
-1, water temperature 18-22 DEG C, photoperiod 12h daytime: cultivate 13-15d under 12h condition at night, all develop into gametophyte and fully after growth, reduce light intensity to 2-10 μ mol photons m wait the trip spore of sprouting
-2s
-1, delay gametophytic growth and make it spend the summer of high water temperature;
5) will be attached to female and male gametophytes on seedling curtain at 50-80 μ mol photons m
-2s
-1under light intensity, complete and grow and fertilization process; Treat that juvenile sporophyte reaches 200-500 μ m left and right, forward marine cultivation to, realize the cultivation of undaria pinnitafida.
Described step 1) and 2) by female gamete body and hermaphroditism gametophyte respectively in PES sea water medium with temperature 18-20 DEG C, intensity of illumination 50-80 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, the static juvenile sporophyte that is cultured to reaches 1mm respectively, and every 3-5 days changes a subculture;
Be cultured to respectively the juvenile sporophyte of 1mm under inflation condition of culture, with temperature 18-20 DEG C, intensity of illumination 50-80 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, be cultured to juvenile sporophyte length and reach 1cm, and change a PES sea water medium for every 2-3 days;
Be cultured to the juvenile sporophyte of 1cm at 4-18 DEG C, utilize nature seawater to be filled with air rolling and cultivate, form sexually matured sporophyte, and within every 2-3 days, upgrade a nature seawater; Wherein, in nature seawater, adding final concentration is 2.5mgL
-1naNO
3with final concentration be 0.25mgL
-1kH
2pO
4.
Described PES medium is in every 1L sterilization seawater, to add 20mL to prepare liquid; Preparing liquid is to add NaNO in every 1L distilled water
33.5g, KH
2pO
40.5g, ferrous metal liquid 250mL and molten metal 250mL; Through dissolving sterilization, cooling stand-by; Ferrous metal liquid is to add Fe (NH in every 1L distilled water
4)
2(SO
4)
26H
2o0.702g and Na
2eDTA0.6g; Molten metal is to add Na in every 1L distilled water
2eDTA1g, H
3bO
31.14g, FeCl
36H
2o49mg, MnSO
4h
2o164mg, ZnSO
47H
2o22mg and CoSO
47H
2o4.8mg.
Described step 3) ripe sporophyte sporophyll is cut off after sterilization removal of impurities algae, 30-60min dries in the shade, sporophyll is put into seawater and diffuse to obtain trip spore, wait diffuse trip spore color intensity of sea water become after yellowish-brown, trip spore is put into seedling curtain, Spore adhesion density is controlled at 60-100 the trip spore adhering under 120 times of multiplication factors, then seedling curtain is put into fresh seawater.
The present invention has advantages of
1. the method for seed selection of the present invention is to induce after the sporophyte maturation of female gamete body clone generation by parthenogenesis, the trip spore discharging is all female trip spore, and it is on all four that the female gamete body forming after sprouting and initial female gamete body are cloned in heredity.
2. selection of the present invention adopts after the sporophyte maturation of hermaphroditic gametophyte selfing generation, and the trip spore of release is all male trip spore, and it is on all four that the male gametophyte forming after sprouting and initial male gametophyte are cloned in heredity.
3. after the sporophyte maturation that the sporophyte that parthenogenesis produces and hermaphroditic gametophyte selfing produce, the trip spore of release and the gametophyte of formation thereof can firmly be attached to Miao Shengshang, and its firm degree can tolerate the flushing of giant.
4. selection of the present invention middle reaches Spore adhesion is after seedling curtain, by the gametophytic growth of two high smooth technology control, aestivate and growth course.
5. the male gametophyte clone hybridization of the trip sporulation that the female gamete body of the trip sporulation that in the present invention, parthenosporophyte maturation discharges afterwards and hermaphroditism gametophyte selfing sporophyte maturation discharge afterwards, the first filial generation sporophyte producing is also highly consistent in heredity, is similar to the single mating combination offspring of gametophyte clone.
6. parthenogenesis of the present invention produces trip spore and parent's female gamete body have on all four genetic background, therefore can replace egagametophyte and carry out fine-variety breeding and seedling fostering.
7. hermaphroditism gametophyte selfing sporophyte of the present invention produces trip spore and parent's male gametophyte have on all four genetic background, therefore can replace male gametophyte and carry out fine-variety breeding and seedling fostering.
Embodiment
Be specially, egagametophyte carried out to parthenogenesis induction and produce sporophyte, meanwhile, hermaphroditic gametophyte is carried out to Development culture and produce selfing sporophyte.When sporophyte reaches 1mm when above, proceed under inflation condition of culture, be cultured to sexual maturity.Ripe sporophyll is placed in to seawater and swims spores release, after color intensity of sea water becomes yellowish-brown, will swim Spore adhesion on seedling curtain, then seedling curtain is placed in to fresh seawater.The trip spore that the sporophyte that parthenogenesis produces discharges is all female trip spore, and the trip spore that the sporophyte that hermaphroditic gametophyte selfing produces discharges is all male trip spore.The female trip spore of gained and male trip Spore adhesion co-incubation on same seedling rope, completes and grows and fertilization process, is equivalent to traditional trip spore collection technique.Treat that juvenile sporophyte reaches 200 μ m left and right, forward marine cultivation to, complete the cultivation of undaria pinnitafida.
Embodiment 1
By female gamete body and hermaphroditic gametophyte respectively in PES sea water medium with 18 DEG C of temperature, intensity of illumination 60 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, and static cultivation, after 20 days, more than sporophyte reaches 1mm, forwards in 2L beaker and is filled with air under the condition of every 3 days replacing one subcultures, with 18 DEG C of temperature, intensity of illumination 60 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, and within every 3 days, change and under the condition of a PES sea water medium, be cultured to juvenile sporophyte length and reach 1cm, the juvenile sporophyte that length is reached to 1cm forwards respectively 80 liters of PP water tanks to and utilizes nature seawater to be filled with air rolling to cultivate that (rolling training method please refer to Pang, S.J., Liu, F., Shan, T.F., Gao, S.Q.and Zhang, Z.H.2009.Cultivation of the brown alga Sargassum horneri:sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature.J.Appl.Phycol.21:413-22.), in the nature seawater of cultivation juvenile sporophyte, add again 2.5mgL
-1naNO
3and 0.25mgL
-1kH
2pO
4, and within every 2 days, upgrade and once add 2.5mgL
-1naNO
3and 0.25mgL
-1kH
2pO
4nature seawater, the seasonal variation scope of water temperature is 4-18 DEG C, and then forms the sporophyte of parthenogenesis maturation and the sporophyte of hermaphroditism selfing maturation.
Described PES medium is in every 1L sterilization seawater, to add 20mL to prepare liquid; Preparing liquid is to add NaNO in every 1L distilled water
33.5g, KH
2pO
40.5g, ferrous metal liquid 250mL and molten metal 250mL; Through dissolving sterilization, cooling stand-by; Ferrous metal liquid is to add Fe (NH in every 1L distilled water
4)
2(SO
4)
26H
2o0.702g and Na
2eDTA0.6g; Molten metal is to add Na in every 1L distilled water
2eDTA1g, H
3bO
31.14g, FeCl
36H
2o49mg, MnSO
4h
2o164mg, ZnSO
47H
2o22mg and CoSO
47H
2o4.8mg.
Treat above-mentioned sporophyte maturation, sporophyll is cut off, with antiseptic gauze wiping sporophyll surface repeatedly, remove the assorted algae of surface attachment, the 30-60min that dries in the shade, puts into seawater by sporophyll and diffuses trip spore, become after yellowish-brown wait discharging the color intensity of sea water that obtains swimming spore, put into the long seedling rope of 16m, Spore adhesion density is controlled at 60-100 the trip spore adhering under 120 times of multiplication factors, afterwards seedling rope is put into fresh seawater.At light intensity 50 μ mol photons m
-2s
-1, water temperature 18-22 DEG C, photoperiod 12h daytime: cultivate 15d under 12h condition at night, the trip spore of sprouting all develops into gametophyte fully growth, reduces afterwards light intensity to 10 μ mol photons m
-2s
-1below, delay gametophytic growth, make it spend the summer of high water temperature.
After sporophyte maturation, the gametophyte that the trip spore of release and growth thereof form can firmly be attached to Miao Shengshang, and its firm degree can tolerate the flushing of giant.
Trip Spore adhesion is after seedling curtain, by the gametophytic growth of two high smooth technology control, aestivate and growth course.Be specially, the first high photophase intensity control is at 50 μ mol photons m
-2s
-1, gametophyte is fully grown, reduce afterwards light intensity to 10 μ mol photons m
-2s
-1below, delay gametophytic growth, spend high water temperature in summer.The second high photophase light intensity is increased to 50 μ mol photons m
-2s
-1above, female and male gametophytes is grown simultaneously, complete fertilization.
The juvenile sporophyte that fertilization forms reaches 200 μ m left and right, forwards the main cultivation area of undaria pinnitafida to---and gold mountain and sea district, Lvshun, Dalian, marine temporary supporting 10 days, then presss from both sides seedling, completes sea and forms.
Embodiment 2
Cultivate if realize large-scale production, what the sporophyte that the sporophyte that parthenogenesis can be produced and hermaphroditism selfing produce was collected seedling in producing plants vegetables.
Treat above-mentioned sporophyte maturation, sporophyll is cut off, with antiseptic gauze wiping sporophyll surface repeatedly, remove the assorted algae of surface attachment, 30-60min dries in the shade, the seawater of sporophyll being put into nursery pond diffuses trip spore, become after yellowish-brown wait the color intensity of sea water that discharges the trip spore obtaining, put into seedling curtain (the long 100m of seedling rope being wound around on each seedling curtain), Spore adhesion density is controlled at 60-100 the trip spore adhering under 120 times of multiplication factors, afterwards seedling curtain is put into fresh seawater.At light intensity 50 μ molphotons m
-2s
-1, water temperature 18-22 DEG C, photoperiod 12h daytime: cultivate 15d under 12h condition at night, the trip spore of sprouting all develops into gametophyte fully growth, reduces afterwards light intensity to 10 μ molphotons m
-2s
-1below, delay gametophytic growth, make it spend the summer of high water temperature.
The gametophyte of trip spore and formation thereof can firmly be attached to Miao Shengshang, and its firm degree can tolerate the flushing of giant.
Male and female trip spore is sprouted and is formed respectively female and male gametophytes simultaneously on seedling rope, through aestivating the stage, light intensity is increased to 50 μ mol photons m
-2s
-1above, complete and grow and fertilization process.When juvenile sporophyte reaches 200 μ m left and right, forward seedling curtain to undaria pinnitafida main cultivation area---gold mountain and sea district, Lvshun, Dalian, marine foster 10 days temporarily, then press from both sides seedling, complete sea and form.By above method, can save required time and the manpower and financial resources of a large amount of cultivation amplification gametophyte.
According to above-mentioned cultural method, from April in October to next year then as a culture-cycle.When next year, measured April, the average length and width of sporophyte, fresh weight are respectively 290.25cm, 72.1cm and 749.75g, and sporophyte offspring economic characters height is consistent.
Claims (4)
1. a method of utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding, is characterized in that:
1) female gamete body is induced and be cultured to juvenile sporophyte and reach 1mm and proceed to above under inflation condition of culture, the history of life that completes parthenosporophyte, form the sporophyte of parthenogenesis maturation;
2) hermaphroditic gametophyte is carried out to Development culture, produce selfing sporophyte, grow to 1mm when above until sporophyte, proceed to and inflate under condition of culture, the history of life that completes selfing sporophyte, form ripe selfing sporophyte;
3) sporophyll of above-mentioned two kinds of ripe sporophytes is placed in to seawater release and obtains else trip spore of two kinds of unicity, after discharging the color intensity of sea water that obtains swimming spore and being yellowish-brown, two kinds of trip spore fluids are mixed and are attached on seedling curtain, then seedling curtain is put into fresh seawater;
4) the above-mentioned seedling curtain of swimming spore that adheres to is at light intensity 50-80 μ mol photons m
-2s
-1, water temperature 18-22 DEG C, photoperiod 12h daytime: cultivate 13-15d under 12h condition at night, all develop into gametophyte and fully after growth, reduce light intensity to 2-10 μ mol photons m wait the trip spore of sprouting
-2s
-1, delay gametophytic growth and make it spend the summer of high water temperature;
5) will be attached to female and male gametophytes on seedling curtain at 50-80 μ mol photons m
-2s
-1under light intensity, complete and grow and fertilization process; Treat that juvenile sporophyte reaches 200-500 μ m left and right, forward marine cultivation to, realize the cultivation of undaria pinnitafida.
2. by the method for utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding claimed in claim 1, it is characterized in that:
Described step 1) and 2) by female gamete body and hermaphroditism gametophyte respectively in PES sea water medium with temperature 18-20 DEG C, intensity of illumination 50-80 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, the static juvenile sporophyte that is cultured to reaches 1mm respectively, and every 3-5 days changes a subculture;
Be cultured to respectively the juvenile sporophyte of 1mm under inflation condition of culture, with temperature 18-20 DEG C, intensity of illumination 50-80 μ mol photons m
-2s
-1, photoperiod 12h daytime: 12h night, be cultured to juvenile sporophyte length and reach 1cm, and change a PES sea water medium for every 2-3 days;
Be cultured to the juvenile sporophyte of 1cm at 4-18 DEG C, utilize nature seawater to be filled with air rolling and cultivate, form sexually matured sporophyte, and within every 2-3 days, upgrade a nature seawater; Wherein, in nature seawater, adding final concentration is 2.5mgL
-1naNO
3with final concentration be 0.25mgL
-1kH
2pO
4.
3. by the method for utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding claimed in claim 1, it is characterized in that: described PES medium is in every 1L sterilization seawater, to add 20mL to prepare liquid; Preparing liquid is to add NaNO in every 1L distilled water
33.5g, KH
2pO
40.5g, ferrous metal liquid 250mL and molten metal 250mL; Through dissolving sterilization, cooling stand-by; Ferrous metal liquid is to add Fe (NH in every 1L distilled water
4)
2(SO
4)
26H
2o0.702g and Na
2eDTA0.6g; Molten metal is to add Na in every 1L distilled water
2eDTA1g, H
3bO
31.14g, FeCl
36H
2o49mg, MnSO
4h
2o164mg, ZnSO
47H
2o22mg and CoSO
47H
2o4.8mg.
4. by the method for utilizing unisexuality trip spore to carry out undaria pinnitafida crossbreeding claimed in claim 1, it is characterized in that: described step 3) ripe sporophyte sporophyll is cut off after sterilization removal of impurities algae, 30-60min dries in the shade, sporophyll is put into seawater and diffuse to obtain trip spore, wait diffuse trip spore color intensity of sea water become after yellowish-brown, trip spore is put into seedling curtain, and Spore adhesion density is controlled at 60-100 the trip spore adhering under 120 times of multiplication factors, then seedling curtain is put into fresh seawater.
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Cited By (6)
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| CN105706893A (en) * | 2016-02-03 | 2016-06-29 | 大连海宝渔业有限公司 | Cloned undaria pinnatifida seedling attachment method |
| CN107950380A (en) * | 2017-12-21 | 2018-04-24 | 山东省农业科学院生物技术研究中心 | A kind of cultivation of undaria pinnitafida and the method for administering red tide |
| CN110771496A (en) * | 2019-11-27 | 2020-02-11 | 山东省海洋生物研究院 | Method for inducing development of low-temperature and low-light preserved kelp male germplasm |
| CN112690208A (en) * | 2020-12-18 | 2021-04-23 | 中国科学院海洋研究所 | Method for constructing Double Haploid (DH) sporophyte population in undaria pinnatifida |
| CN112913678A (en) * | 2020-05-15 | 2021-06-08 | 中国科学院海洋研究所 | A method for preparing permanent F from Undaria Pinnatifida2Methods of sporozoite population |
| CN112956413A (en) * | 2021-01-28 | 2021-06-15 | 大连海宝渔业有限公司 | Method for preventing benthic diatom pollution in undaria pinnatifida gametophyte seedling culture production |
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| CN1080114A (en) * | 1992-06-15 | 1994-01-05 | 中国科学院海洋研究所 | Haploid clone breeding technology for undaria pinnatifida |
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