CN104090117A - Switch-control-type paper microfluidic chip for detecting clenbuterol hydrochloride based on SERS (surface-enhanced raman scatting) - Google Patents
Switch-control-type paper microfluidic chip for detecting clenbuterol hydrochloride based on SERS (surface-enhanced raman scatting) Download PDFInfo
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- CN104090117A CN104090117A CN201410328800.6A CN201410328800A CN104090117A CN 104090117 A CN104090117 A CN 104090117A CN 201410328800 A CN201410328800 A CN 201410328800A CN 104090117 A CN104090117 A CN 104090117A
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Abstract
The invention belongs to the technical field of food safety inspection and discloses a switch-control-type paper microfluidic chip for detecting clenbuterol hydrochloride based on SERS (surface-enhanced raman scatting). The switch-control-type paper microfluidic chip mainly comprises a chip main body, a first paper tape switch and a second paper tape switch, wherein a main hydrophilic channel uncoated with wax is formed in the fluidic chip main body; N groups of chip units in parallel arrangement are arranged on two sides of the main hydrophilic channel; sample feeding areas, antibody feeding areas, signal detection areas and baffle areas are formed in each group of chip units; the sample feeding areas, the antibody feeding areas and the signal detection areas on two sides of the main hydrophilic channel are respectively communicated through two secondary hydrophilic channels which are uncoated with wax; an included angle between each of the two secondary hydrophilic channels and the main hydrophilic channel is 0 to 90 degrees. Hydrophobic areas coated with wax and hydrophilic areas which are defined by the hydrophobic areas and uncoated with wax are respectively formed in the first paper tape switch and the second paper tape switch; the two paper switches are respectively inserted in the fluidic chip main body at intervals along with dotted lines which are drawn in advance; the paper tape switches are switched on and off by pulling paper tapes. The switch-control-type paper microfluidic chip is used for detecting clenbuterol hydrochloride based on SERS in the site, is high in detection speed, accurate in detection and simple and convenient to operate.
Description
Technical field
The invention belongs to food safety detection technical field, relate to a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on Surface enhanced raman spectroscopy (SERS, surface-enhanced Raman scatting).
Background technology
Clenbuterol hydrochloride is a parahormone, belongs to beta-receptor activator, comprises clenobuterol hydrochloride, Ractopamine, salbutamol etc.Because this class material makes an addition to the body weight that can increase animal in feed, make its muscle more solid.So a lot of raisers use for reducing feed, make meat listing ahead of time, reduce costs and clenbuterol hydrochloride is used in aquaculture, even arrive the stage of abusing.And people has eaten after the meat product that contains beta-receptor activator, the symptom such as can produce muscle twitches, vomiting, neuroticism and palpitate quickly.So such material has been listed in the growth promoter class material of forbidding in aquaculture.For preventing and supervise illegal retailer and the raiser use to this type of material, indispensable in the food safety detection market of China to the detection analysis of clenbuterol hydrochloride.
At present, conventional clenbuterol hydrochloride detection means comprises two classes: (1) colloidal gold strip method; (2) enzyme linked immunosorbent assay, High Performance Liquid Chromatography/Mass Spectrometry method and gas chromatography-mass spectrography etc.First kind method false positive rate is very high, and Equations of The Second Kind method can only be carried out in centralab, can not accomplish instant Site Detection.
Refill sheet (Paper-based microfluidics) is the In Microfluidic Analytical Systems based on paper material, as the newcomer of microfluidic chip system, in quick diagnosis and on-the site analysis, show up prominently, and receive increasing concern.Paper material is inert fiber element, cheap, and recyclable regenerative is easy to storage and transport, and stability and biocompatibility are all fine.On paper, manufacture hydrophobic barrier and form the papery micro fluidic device that this method of hydrophilic channel builds and can realize sample separation, the function of filtration, meanwhile, relies on capillary force or Action of Gravity Field that sample is moved, easy to operate.Consider the detection demand that vast detection is analyzed market and further expanded, how better refill sheet detection platform need exploitation.
Papery micro-fluidic chip has very large potentiality aspect the improvement of clenbuterol hydrochloride detection technique, its innovative research has great significance for the instant on-the-spot high flux reliable detection that better realizes clenbuterol hydrochloride.
Summary of the invention
The deficiency existing in order to overcome prior art, the object of the invention is to provide a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on SERS, this papery micro-fluidic chip is based on SERS, clenbuterol hydrochloride class material to be detected, and detection speed is fast, accurate, easy to operate.In addition, cheap, the recyclable regenerative of material price that papery micro-fluidic chip adopts, be easy to storage and transport, stability and biocompatibility are all fine.
In order to realize foregoing invention object, solve the problem existing in prior art, the technical scheme that the present invention takes is: a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on SERS, mainly comprise chip body, the first paper slip switch in the time of off-state and the second paper slip switch in the time of on-state, main hydrophilic channel without wax spray is set in described chip body, the described main hydrophilic channel both sides without wax spray arrange N group parallel arranged, symmetrical chip unit, wherein N is 1, 2, 3 ... positive integer, described every core assembly blade unit arranges sample feeding district, antibody sample introduction district, input district and a baffle area that hydrophobic barrier forms, the sample feeding district of the described main hydrophilic channel both sides without wax spray, antibody sample introduction district and input district are communicated with by two secondary hydrophilic channels without wax spray respectively, described two without the secondary hydrophilic channel of wax spray be 0-90 ° of angle without the main hydrophilic channel of wax spray, on described the first paper slip switch in the time of off-state, the second paper slip switch in the time of on-state, arrange respectively through the hydrophobic region of wax spray and the hydrophilic region without wax spray surrounding thereof, two paper slip switches alternate in chip body along drawn in advance dotted line position respectively, rely on extruding between the two with chip body and be closely connected for paper slip switch, by pulling paper slip to complete the connecting and disconnecting of paper slip switch, hydrophobic region on described the first paper slip switch in the time of off-state is connected with sample feeding district, hydrophilic region on described the second paper slip switch in the time of on-state is communicated with antibody sample introduction district and input district, for the filtration to sample, enrichment with separate.
The material of described papery micro-fluidic chip is selected from the one in qualitative filter paper, quantitative filter paper, printer paper or nitrocellulose filter.
Beneficial effect of the present invention is: a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on SERS, mainly comprise chip body, the first paper slip switch in the time of off-state and the second paper slip switch in the time of on-state, main hydrophilic channel without wax spray is set in described chip body, the described main hydrophilic channel both sides without wax spray arrange N group parallel arranged, symmetrical chip unit, wherein N is 1, 2, 3 ... positive integer, described every core assembly blade unit arranges sample feeding district, antibody sample introduction district, input district and a baffle area that hydrophobic barrier forms, the sample feeding district of the described main hydrophilic channel both sides without wax spray, antibody sample introduction district and input district are communicated with by two secondary hydrophilic channels without wax spray respectively, described two without the secondary hydrophilic channel of wax spray be 0-90 ° of angle without the main hydrophilic channel of wax spray, on described the first paper slip switch in the time of off-state, the second paper slip switch in the time of on-state, arrange respectively through the hydrophobic region of wax spray and the hydrophilic region without wax spray surrounding thereof, two paper slip switches alternate in chip body along drawn in advance dotted line position respectively, rely on extruding between the two with chip body and be closely connected for paper slip switch, by pulling paper slip to complete the connecting and disconnecting of paper slip switch, hydrophobic region on described the first paper slip switch in the time of off-state is connected with sample feeding district, hydrophilic region on described the second paper slip switch in the time of on-state is communicated with antibody sample introduction district and input district, for the filtration to sample, enrichment with separate.Compared with the prior art, the present invention detects clenbuterol hydrochloride class material at the scene based on SERS, and detection speed is fast, accurate, easy to operate.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is the SERS spectrogram of variable concentrations clenbuterol hydrochloride of the present invention.
In figure: 1, the first paper slip switch in the time of off-state, 1a, the first paper slip switch hydrophilic region, 1b, the first paper slip switch hydrophobic region, 2, the second paper slip switch in the time of on-state, 2a, the second paper slip switch hydrophilic region, 2b, the second paper slip switch hydrophobic region, 3, chip body, 3a, the hydrophobic barrier forming through wax spray, 3b, antibody sample introduction district, 3c, without the secondary hydrophilic channel of wax spray, 3d, input district, 3e, sample feeding district, 3f, form through wax spray the baffle area that hydrophobic barrier forms, 3g, chip body need to be scratched the gap of inserting paper slip switch, 3h, without the main hydrophilic channel of wax spray.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
As shown in Figure 1, a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on SERS, mainly comprise chip body 3, the first paper slip switch 1 in the time of off-state and the second paper slip switch 2 in the time of on-state, main hydrophilic channel 3h without wax spray is set in described chip body 3, the described main hydrophilic channel 3h both sides without wax spray arrange N group parallel arranged, symmetrical chip unit, wherein N is 1, 2, 3 ... positive integer, described every core assembly blade unit arranges sample feeding district 3e, antibody sample introduction district 3b, input district 3d and a baffle area 3f that hydrophobic barrier forms, the sample feeding district 3e of the described main hydrophilic channel 3h both sides without wax spray, antibody sample introduction district 3b and input district 3d are communicated with by two secondary hydrophilic channel 3c without wax spray respectively, described two without the secondary hydrophilic channel 3c of wax spray be 0-90 ° of angle without the main hydrophilic channel 3h of wax spray, on described the first paper slip switch 1 in the time of off-state, the second paper slip switch 2 in the time of on-state, arrange respectively through hydrophobic region 1b, the 2b of wax spray and hydrophilic region 1a, the 2a without wax spray surrounding thereof, two paper slip switches alternate in chip body 3 along drawn in advance dotted line position 3g respectively, rely on extruding between the two with chip body and be closely connected for paper slip switch, by pulling paper slip to complete the connecting and disconnecting of paper slip switch, hydrophobic region 1b on described the first paper slip switch 1 in the time of off-state is connected with sample feeding district 3e, hydrophilic region 2a on described the second paper slip switch 2 in the time of on-state is communicated with antibody sample introduction district 3b and input district 3d, for the filtration to sample, enrichment with separate.The material of described papery micro-fluidic chip is selected from the one in qualitative filter paper, quantitative filter paper, printer paper or nitrocellulose filter.Specific operation process is as follows: first the chip design designing is printed on filter paper with wax spray printer, and toasts the inside that makes wax be penetrated into paper in 3 minutes under 150 DEG C of conditions, form hydrophobic cofferdam.Then the left margin in input district, right margin and lower boundary are cut off, and whole region are holded up and chip body plane is 90 ° of angles, and sample can not spread to surrounding after adding like this, size that can control signal surveyed area.Dotted line on chip body two wing passages is scratched, two paper slip switches are inserted to chip body, now should be noted that and guarantee that the hydrophilic region of paper slip switch can not be overlapping with the hydrophobic region of chip body, in order to avoid at this moment with regard to too early communications signals detection zone and sample feeding district or antibody sample introduction district.Then in input district, drip shitosan and pentanedial decoration, drip afterwards 2 μ L clenobuterol hydrochloride antigens, and drip bovine serum albumen solution block site.Again input district is pushed back to paper chip body institute planar again, the determinand solution of the Clenbuterol that drips 20 μ L antibody in antibody sample introduction district and sample feeding district respectively and contain variable concentrations, pull paper slip switch, make the hydrophilic region of white on paper slip cover the hydrophobic region of the black in chip body, and be closely affixed, play connection effect, make the sample on both sides and antibody flow or be diffused into input district fully reaction.Hair particle deposition in determinand is in sample feeding district, and sample arrives input district through the filtration of paper slip switch.Mobile and the rate of propagation that can observe antibody and sample is adjusted the time of two paper slip switch opens, makes the two arriving signal detection zone simultaneously.Three core assembly blade units of parallel arranged can be measured respectively the sample of variable concentrations, and now antibody sample introduction district is same antibody; Or also can detect three kinds of antigen molecules, at this moment need three kinds of corresponding different antibodies.After immune response, wash away unconjugated antibody, input district detects Raman signal afterwards, and along with testing concentration increases, Raman signal weakens, and detects and is limited to 10pg/mL.
Claims (2)
1. one kind is detected the switch controlled papery micro-fluidic chip of clenbuterol hydrochloride class material based on SERS, mainly comprise chip body (3), the first paper slip switch (1) in the time of off-state and the second paper slip switch (2) in the time of on-state, it is characterized in that: the upper main hydrophilic channel (3h) arranging without wax spray of described chip body (3), described main hydrophilic channel (3h) both sides without wax spray arrange N group parallel arranged, symmetrical chip unit, wherein N is 1, 2, 3 ... positive integer, described every core assembly blade unit arranges sample feeding district (3e), antibody sample introduction district (3b), input district (3d) and a baffle area (3f) that hydrophobic barrier forms, the sample feeding district (3e) of described main hydrophilic channel (3h) both sides without wax spray, antibody sample introduction district (3b) and input district (3d) are communicated with by two secondary hydrophilic channels (3c) without wax spray respectively, described two without the secondary hydrophilic channel (3c) of wax spray be 0-90 ° of angle without the main hydrophilic channel (3h) of wax spray, the hydrophilic region without wax spray (1a, 2a) that hydrophobic region (1b, 2b) through wax spray is set respectively and surrounds on described the first paper slip switch (1) in the time of off-state, the second paper slip switch (2) in the time of on-state, two paper slip switches alternate in chip body (3) along drawn in advance dotted line position (3g) respectively, rely on extruding between the two with chip body and be closely connected for paper slip switch, by pulling paper slip to complete the connecting and disconnecting of paper slip switch, hydrophobic region (1b) on described the first paper slip switch (1) in the time of off-state is connected with sample feeding district (3e), hydrophilic region (2a) on described the second paper slip switch (2) in the time of on-state is communicated with antibody sample introduction district (3b) and input district (3d), for the filtration to sample, enrichment with separate.
2. a kind of switch controlled papery micro-fluidic chip that detects clenbuterol hydrochloride class material based on SERS according to claim 1, is characterized in that: the material of described papery micro-fluidic chip is selected from the one in qualitative filter paper, quantitative filter paper, printer paper or nitrocellulose filter.
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| WO2016156783A1 (en) * | 2015-03-27 | 2016-10-06 | University Court Of The University Of St Andrews | Pharmaceutical detection |
| CN108291904A (en) * | 2015-11-19 | 2018-07-17 | 赛多利斯史泰迪生物技术有限责任公司 | Patterned membrane structure |
| CN110261605A (en) * | 2019-06-25 | 2019-09-20 | 陕西科技大学 | A kind of method of chitin modified enhancing test paper detection sensitivity |
| CN110354920A (en) * | 2018-03-26 | 2019-10-22 | 首都师范大学 | A kind of enrichment detecting method of paper substrate micro-fluidic chip and the phosphorylated polypeptide based on it |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110261605A (en) * | 2019-06-25 | 2019-09-20 | 陕西科技大学 | A kind of method of chitin modified enhancing test paper detection sensitivity |
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