CN104087568A - Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment - Google Patents
Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment Download PDFInfo
- Publication number
- CN104087568A CN104087568A CN201410259576.XA CN201410259576A CN104087568A CN 104087568 A CN104087568 A CN 104087568A CN 201410259576 A CN201410259576 A CN 201410259576A CN 104087568 A CN104087568 A CN 104087568A
- Authority
- CN
- China
- Prior art keywords
- preparation
- liquid
- hemicellulase
- potato
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/10—Bleaching ; Apparatus therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses preparation and application of hemicellulase for pulping papermaking bleaching pretreatment. The method comprises the following steps: liquid seeds are obtained by test tube slant activation and liquid culture way; a carbon source, a nitrogen source, glucose and mandles nutrient fluid are mixed evenly for 1h of sterilization at 121 DEG C, and then transferred into a fermentation tank after cooling; the liquid seed are put into the fermentation tank for fermentation to obtain a hemicellulase solution. The method uses corncob, wheat bran, chitosan, crude hemicellulose and other biomass base materials as inducing substrates, saves resources and is very friendly to the environment. The hemicellulase solution obtained by the method can fully adapt to the pulping papermaking industry application requirement, and can greatly increase the whiteness of pulp whiteness after use.
Description
Technical field
The invention belongs to and belong to bio-bleaching field, be specifically related to preparation and the application of hemicellulase for a kind of pulping and paper-making bleaching pre-treatment.
Background technology
In pulp and paper industry, the bleaching of paper pulp is very important workshop section.Traditionally, pulp and paper industry generally adopts chloride SYNTHETIC OPTICAL WHITNER to bleach.Although its bleaching effect is good, when using the bleaching of this method, can makes to produce in paper waste organic chloride a large amount of, poisonous, deformity, thereby can cause serious harm to environment.Due to the requirement of environment, papermaking enterprise must address this problem, the non-polluting bleachoing technology of Development of Novel.Therefore traditional chlorine bleaching is sent out to a great extent and is replaced by element chlorine free bleaching (ECF) and total chlorine free bleaching (TCF) (TCF).Hydrogen peroxide bleaching is cleaning and bleaching technology common in paper industry.Hydrogen peroxide bleaching does not remove the residual lignin in slurry, but by destroying to a certain extent the chromophoric groups such as the conjugation carbonyl of lignin and o-quinone, makes it become not chromophoric group and reach bleaching effect.Hydrogen peroxide bleaching is effective, and environmentally safe does not produce the derivative of chlorine in pulp suspension body, meets environmental requirement.If before bleaching, with hemicellulase, paper pulp is carried out to pre-treatment, will make the pharmaceutical chemicalss such as hydrogen peroxide better be penetrated into paper pulp the inside, thereby improve bleaching efficiency, further reduce and pollute.Recent domestic is in the ascendant to the research of pulp bio technology, and wherein as bleach-activating agent, short bleaching becomes and in paper industry, has one of problem of development prospect most microbial enzyme.Adopt biomass preconditioning technique, not only can improve the physicals of paper pulp and can reduce the pollution load of bleaching effluent.
Hemicellulose accounts for 1/3rd of plant carbohydrates total amount, at occurring in nature, is the abundant renewable physical resources of content second after Mierocrystalline cellulose.Hemicellulase is that a kind of prozyme is, comprises zytase, poly lactose enzyme etc.The research of hemicellulase was just started as far back as the sixties, from the microorganism of different sources, be separated to half cellulase of a large amount of dissimilar, difference in functionalitys.Due to the development of Gene science, the application of hemicellulase in paper industry caused the extensive concern of scientists in recent years.
Summary of the invention
For solving the shortcoming and defect part of prior art, primary and foremost purpose of the present invention is to provide the preparation method of a kind of pulping and paper-making bleaching pre-treatment with hemicellulase.
Another object of the present invention is to provide the application of the hemicellulase that above-mentioned preparation method obtains.
For achieving the above object, the present invention adopts following technical scheme: the preparation method of hemicellulase for a kind of pulping and paper-making bleaching pre-treatment, comprises the following steps:
(1) ATCC56765 Trichodermareesei bacterial classification is placed in to potato glucose G/W, at 25～35 ℃, under 180rpm condition, cultivates 48 hours, produce spore; Then the spore inoculating making is carried out to solid culture in potato dextrose agar (PDA) substratum, under 25～35 ℃ of conditions, cultivate 1～4 day, produce well-developed single bacterium colony;
(2) well-developed single bacterium colony step (1) being made is placed in potato glucose water and carries out liquid culture, under 30 ℃, 150～220rpm condition, cultivates 48 hours, turns out liquid seeds;
(3) carbon source, nitrogenous source, glucose and mandles nutritive medium are mixed after under 121 ℃ of conditions sterilizing within 1 hour, obtain substrate, after substrate is cooling, proceed to fermentor tank, the liquid seeds that step (2) is made is inoculated into the fermentation cylinder for fermentation that installs substrate and cultivates 1～4 day, obtains bacterium liquid;
(4) after bacterium liquid centrifugation step (3) being obtained, collect clear liquid, obtain the crude extract of enzyme; By carrying out chromatography purification after crude extract dialysis, obtain hemicellulase liquid.
Preferably, the consisting of of step (1) described potato dextrose agar: 20% potato juice, glucose, potassium primary phosphate, bitter salt, VitB1 and agar, its ratio is 1L:20g:3g:1.5g:8mg:20g; Described potato dextrose agar pH value is 4～6.
Preferred, described 20% potato juice compound method is: remove skin potato 200g, be cut into small pieces, add 1L water, boil 20min, elimination potato ball, then adds water filtrate is supplied to 1L, obtains described 20% potato juice.
Preferably, the inoculum size of the described liquid seeds of step (3) is 1～5% (lmL～5mL/100mL).
Preferably, the described carbon source of step (3) is corn cob, wheat bran, chitosan, thick hemicellulose, or a kind of in corn cob, wheat bran, chitosan and thick hemicellulose enzyme mixture; Described nitrogenous source is extractum carnis.
Preferably, in step (3), the actual conditions of fermentation culture is: tank pressure 0.01～0.08MPa, initial air quantity is 800m
3/ h, cultivates air quantity after 4 hours and opens to 1500m
3/ h, initial mixing speed is 120r/min; Cultivate mixing speed after 4 hours and be adjusted to 180r/min, in culturing process, add buffered soln pH value is maintained to 4～6, yeast culture temperature is 25 ℃～35 ℃, when stablizing, pH adds methyl alcohol when constant, methyl alcohol addition is 1% (per-cent of methanol quality and fermentating liquid volume), and in this process, keeping temperature is 25 ℃～35 ℃.
Preferred, in culturing process, add buffered soln pH value is maintained to 5.5.
Preferably, described in step (3), the addition of carbon source, nitrogenous source, glucose and mandles nutritive medium is respectively 10～20g/L, 10～20g/L, 0.1～0.5% and 0.01～0.10%, and percentage ratio is wherein glucose or the quality of mandles nutritive medium and the per-cent of fermentating liquid volume.
Preferred, described buffered soln is SODIUM PHOSPHATE, MONOBASIC or Sodium phosphate dibasic; The air passing in described fermentor tank, the buffered soln of adding and methyl alcohol all pass through sterilising treatment.
Preferably, adopt in return medium of CM-Sephadex in step (4), the phosphoric acid buffer that pH value is 6.0 carries out cation-exchange chromatography to crude extract.
The hemicellulase that aforesaid method makes can be for pulping and paper-making bleaching field.
Compared with prior art, the present invention has the following advantages and beneficial effect:
(1) the inventive method adopts and utilizes the Trichodermareesei of modification as fermented bacterium, and the hemicellulase liquid cellulase activity that this bacterial classification produces is low, can prevent a large amount of degradeds of fiber in pulp bleaching process.
(2) the inventive method adopts the biomass-based materials such as corn cob, wheat bran, chitosan, thick hemicellulose as induction substrate, has saved resource simultaneously very friendly to environment.
(3) the hemicellulase liquid that the inventive method is produced can fully adapt to pulp and paper industry and use requirement, after using, can significantly improve pulp brightness simultaneously.
Accompanying drawing explanation
Fig. 1 is the preparation method's of hemicellulase preparation flow figure for pulping and paper-making bleaching pre-treatment of the present invention.
Fig. 2 is that in the present invention, different carbon sources are produced the result schematic diagram that affects of hemicellulase on Trichodermareesei bacterial classification.Wherein substrate 1 is that corn cob, 2 is that wheat bran, 3 is that chitosan, 4 is the corn cob after sieving, wheat bran, chitosan and thick hemicellulose enzyme mixture.
Fig. 3 is that in the present invention, carbon source Different adding amount is produced the result schematic diagram that affects of hemicellulase on Trichodermareesei bacterial classification.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
The Trichodermareesei bacterial classification that the present invention uses, purchased from USDA DSMZ, is numbered ATCC56765, and in following embodiment, material therefor is commercially available obtaining.
Embodiment 1
A preparation method for hemicellulase for pulping and paper-making bleaching pre-treatment, as shown in Figure 1, comprises the following steps:
(1) slant strains is cultivated: ATCC56765 Trichodermareesei bacterial classification is placed in to potato glucose G/W, at 30 ℃, cultivates 48 hours under 180rpm condition, produce spore; Then the spore inoculating making is carried out to solid culture in potato dextrose agar (PDA) substratum, at 30 ℃, place 48h, produce well-developed single bacterium colony, put into afterwards refrigerator freezing and preserve; PDA slant medium used comprises: 20% potato juice 1L, and glucose 20g, potassium primary phosphate 3g, seven water and magnesium sulfate 1.5g, VitB1 8mg and agar 20g, medium pH is 6.0;
Described 20% potato juice compound method is: remove skin potato 200g, be cut into small pieces, add 1L water, boil 20min, elimination potato ball, then adds water filtrate is supplied to 1L, obtains described 20% potato juice;
(2) shaking flask amplification culture: well-developed single bacterium colony that step (1) is made is placed in containing the triangular flask of 500mL potato glucose water and cultivates, at 30 ℃, 180rpm overnight incubation, turns out liquid seeds;
(3) be seeded to fermentor cultivation: after carbon source (corn cob), nitrogenous source (extractum carnis), glucose and mandles nutritive medium are mixed, sterilizing obtains substrate for 1 hour under 121 ℃ of conditions, after substrate is cooling, proceed to fermentor tank, the liquid seeds inoculation (inoculum size 4%) simultaneously step (2) being made, to installing in the fermentor tank of substrate, is settled to 10m by raw material in tank
3, directly pass into sterilised air, guarantee that air is at 12m
3, regulating pH to 5.5, fermentation culture 4 days, obtains bacterium liquid;
Fermentation culture actual conditions is: tank pressure 0.05MPa, initial air quantity is 800m
3/ h, cultivates air quantity after 4 hours and opens to 1500m
3/ h guarantees that dissolved oxygen amount is greater than 20% above, and fermentation starting stage mixing speed is 120r/min; Cultivate mixing speed after 4 hours and be adjusted to 180r/min, add at any time buffered soln pH value is maintained to 5.5 in culturing process, yeast culture temperature is 30 ℃;
After inoculation, by fermentation, started, pH value of mensuration per hour, makes pH value maintain 5.5 left and right, cultivate after 48 hours by control to start to measure enzyme alive, to pH, add methanol induction after without obvious decline, to improve the output of enzyme;
(4) after bacterium liquid centrifugation step (3) being obtained, collect clear liquid, obtain the crude extract of enzyme; By carrying out chromatography purification after crude extract dialysis, obtain described hemicellulase liquid.
Wherein Carbon and nitrogen sources addition is 10～20g/L, and the add-on of glucose and mandles nutritive medium is respectively 0.1～0.5% (per-cent of quality and fermentating liquid volume) and 0.01～0.1% (per-cent of quality and fermentating liquid volume).In final hemicellulase liquid, enzyme is lived as 1423.59IU/g.
Embodiment 2: as described in Example 1, when difference is the solid culture of step (1), culture temperature is 25 ℃ to concrete steps; While preparing liquid seeds in step (2), rotating speed is 150rpm, and incubation time is 1 day.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 1223.59IU/g.
Embodiment 3: as described in Example 1, when difference is the solid culture of step (1), culture temperature is 35 ℃ to concrete steps; While preparing liquid seeds in step (2), rotating speed is 220rpm, and incubation time is 4 days.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 1323.59IU/g.
Embodiment 4: as described in Example 1, difference is that the carbon source adding in step (3) fermenting process is wheat bran to concrete steps.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 368IU/g.
Embodiment 5: as described in Example 1, difference is that the carbon source adding in step (3) fermenting process is chitosan to concrete steps.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 57.35IU/g.
Embodiment 6
A preparation method for hemicellulase for pulping and paper-making bleaching pre-treatment, comprises the following steps:
(1) slant strains is cultivated: ATCC56765 Trichodermareesei bacterial classification is placed in to potato glucose G/W, at 30 ℃, cultivates 48 hours under 180rpm condition, produce spore; Then the spore inoculating making is carried out to solid culture in potato dextrose agar (PDA) substratum, at 30 ℃, place 48h, produce well-developed single bacterium colony, put into afterwards refrigerator freezing and preserve; PDA slant medium used comprises: 20% potato juice 1L, and glucose 20g, potassium primary phosphate 3g, seven water and magnesium sulfate 1.5g, VitB1 8mg and agar 20g, medium pH is 6.0;
Described 20% potato juice compound method is: remove skin potato 200g, be cut into small pieces, add 1L water, boil 20min, elimination potato ball, then adds water filtrate is supplied to 1L, obtains described 20% potato juice;
(2) shaking flask amplification culture: well-developed single bacterium colony that step (1) is made is placed in containing the triangular flask of 500mL potato glucose water and cultivates, at 30 ℃, 180rpm overnight incubation, turns out liquid seeds;
(3) be seeded to fermentor cultivation: after carbon source (corn cob, wheat bran, chitosan and thick hemicellulose enzyme mixture are crossed 100 mesh sieve gained), nitrogenous source (extractum carnis), glucose and mandles nutritive medium are mixed, sterilizing obtains substrate for 1 hour under 121 ℃ of conditions, after substrate is cooling, proceed to fermentor tank, the liquid seeds inoculation (inoculum size 4%) simultaneously step (2) being made, to installing in the fermentor tank of substrate, is settled to 10m by raw material in tank
3, directly pass into sterilised air, guarantee that air is at 12m
3, regulating pH to 4～6, fermentation culture 4 days, obtains bacterium liquid;
Fermentation culture actual conditions is: tank pressure 0.05MPa, initial air quantity is 800m
3/ h, cultivates air quantity after 4 hours and opens to 1500m
3/ h guarantees that dissolved oxygen amount is greater than 20% above, and fermentation starting stage mixing speed is 120r/min; Cultivate mixing speed after 4 hours and be adjusted to 180r/min, add at any time buffered soln pH value is maintained to 4～6 in culturing process, yeast culture temperature is 30 ℃;
After inoculation, by fermentation, started, pH value of mensuration per hour, makes pH value maintain 4～6 left and right, cultivate after 48 hours by control to start to measure enzyme alive, to pH, add methanol induction after without obvious decline, to improve the output of enzyme;
(4) after bacterium liquid centrifugation step (3) being obtained, collect clear liquid, obtain the crude extract of enzyme; By carrying out chromatography purification after crude extract dialysis, obtain described hemicellulase liquid.
Wherein Carbon and nitrogen sources addition is 10g/L, and the add-on of glucose and mandles nutritive medium is respectively 0.1～0.5% (per-cent of quality and fermentating liquid volume) and 0.01～0.1% (per-cent of quality and fermentating liquid volume).In final gained hemicellulase liquid, enzyme is lived as 2137.46IU/g.
Embodiment 7: as described in Example 6, when difference is the solid culture of step (1), culture temperature is 25 ℃ to concrete steps; While preparing liquid seeds in step (2), rotating speed is 150rpm, and incubation time is 1 day.In final hemicellulase liquid, enzyme is lived as 1923.59IU/g.
Embodiment 8: as described in Example 6, when difference is the solid culture of step (1), culture temperature is 35 ℃ to concrete steps; While preparing liquid seeds in step (2), rotating speed is 220rpm, and incubation time is 4 days.In final gained hemicellulase liquid, enzyme is lived as 1723.59IU/g.
Embodiment 9: as described in Example 6, difference is that the carbon source amount adding in step (3) fermenting process is 5g/L to concrete steps.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 958.14IU/g.
Embodiment 10: as described in Example 6, difference is that the carbon source amount adding in step (3) fermenting process is 20g/L to concrete steps.In the hemicellulase liquid of final the present embodiment gained, enzyme is lived as 1548.25IU/g.
Fig. 2 is that in the present invention, different carbon sources are produced the result schematic diagram that affects of hemicellulase on Trichodermareesei bacterial classification.Wherein substrate 1 is that corn cob, 2 is that wheat bran, 3 is that chitosan, 4 is the corn cob after sieving, wheat bran, chitosan and thick hemicellulose enzyme mixture.When substrate is the 4th kind as can be seen from FIG., enzyme is lived the highest, but while complicated operation loses higher.Considering cost factor, best substrate is corn cob.
Fig. 3 is that in the present invention, carbon source Different adding amount is produced the result schematic diagram that affects of hemicellulase on Trichodermareesei bacterial classification.When carbon source addition is 10～14g/L as can be seen from FIG., the activity of enzymatic production is the highest.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. a preparation method for hemicellulase for pulping and paper-making bleaching pre-treatment, is characterized in that, comprises the following steps:
(1) ATCC56765 Trichodermareesei bacterial classification is placed in to potato glucose G/W, at 25～35 ℃, under 180rpm condition, cultivates 48 hours, produce spore; Then the spore inoculating making is carried out to solid culture in potato dextrose agar, under 25～35 ℃ of conditions, cultivate 1～4 day, produce well-developed single bacterium colony;
(2) well-developed single bacterium colony step (1) being made is placed in potato glucose water and carries out liquid culture, under 30 ℃, 150～180rpm condition, cultivates 48 hours, turns out liquid seeds;
(3) carbon source, nitrogenous source, glucose and mandles nutritive medium are mixed after under 121 ℃ of conditions sterilizing within 1 hour, obtain substrate, after substrate is cooling, proceed to fermentor tank, the liquid seeds that step (2) is made is inoculated into the fermentation cylinder for fermentation that installs substrate and cultivates 1～4 day, obtains bacterium liquid;
(4) after bacterium liquid centrifugation step (3) being obtained, collect clear liquid, obtain the crude extract of enzyme; By carrying out chromatography purification after crude extract dialysis, obtain hemicellulase liquid.
2. preparation method according to claim 1, it is characterized in that, consisting of of step (1) described potato dextrose agar: 20% potato juice, glucose, potassium primary phosphate, bitter salt, VitB1 and agar, its ratio is 1L:20g:3g:1.5g:8mg:20g; Described potato dextrose agar pH value is 4～6.
3. preparation method according to claim 2, is characterized in that, described 20% potato juice compound method is: remove skin potato 200g, be cut into small pieces, add 1L water, boil 20min, elimination potato ball, then adds water filtrate is supplied to 1L, obtains described 20% potato juice.
4. preparation method according to claim 1, is characterized in that, the inoculum size of the described liquid seeds of step (3) is 1～5%.
5. preparation method according to claim 1, is characterized in that, the described carbon source of step (3) is corn cob, wheat bran, chitosan, thick hemicellulose, or a kind of in corn cob, wheat bran, chitosan and thick hemicellulose enzyme mixture; Described nitrogenous source is extractum carnis.
6. preparation method according to claim 1, it is characterized in that, described in step (3), the addition of carbon source, nitrogenous source, glucose and mandles nutritive medium is respectively 10～20g/L, 10～20g/L, 0.1～0.5% and 0.01～0.10%, and percentage ratio is wherein glucose or the quality of mandles nutritive medium and the per-cent of fermentating liquid volume.
7. preparation method according to claim 1, is characterized in that, in step (3), the actual conditions of fermentation culture is: tank pressure 0.01～0.08MPa, initial air quantity is 800m
3/ h, cultivates air quantity after 4 hours and opens to 1500m
3/ h, initial mixing speed is 120r/min; Cultivate mixing speed after 4 hours and be adjusted to 180r/min, add buffered soln pH value is maintained to 5.5 in culturing process, yeast culture temperature is 25 ℃～35 ℃, when pH stablizes, adds methyl alcohol when constant.
8. preparation method according to claim 7, is characterized in that, described buffered soln is SODIUM PHOSPHATE, MONOBASIC or Sodium phosphate dibasic; The air passing in described fermentor tank, the buffered soln of adding and methyl alcohol all pass through sterilising treatment.
9. preparation method according to claim 1, is characterized in that, adopts in return medium of CM-Sephadex in step (4), and the phosphoric acid buffer that pH value is 6.0 carries out cation-exchange chromatography to crude extract.
10. the application of the hemicellulase that described in claim 1, preparation method obtains in pulping and paper-making bleaching field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410259576.XA CN104087568A (en) | 2014-06-11 | 2014-06-11 | Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410259576.XA CN104087568A (en) | 2014-06-11 | 2014-06-11 | Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104087568A true CN104087568A (en) | 2014-10-08 |
Family
ID=51635329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410259576.XA Pending CN104087568A (en) | 2014-06-11 | 2014-06-11 | Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104087568A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103154249A (en) * | 2010-06-04 | 2013-06-12 | 国家技术研究中心Vtt | Method for improved protein production in filamentous fungi |
-
2014
- 2014-06-11 CN CN201410259576.XA patent/CN104087568A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103154249A (en) * | 2010-06-04 | 2013-06-12 | 国家技术研究中心Vtt | Method for improved protein production in filamentous fungi |
Non-Patent Citations (3)
| Title |
|---|
| 无: "里氏木霉RUTC30生产半纤维素酶", 《中国造纸学会第九届学术年会论文集》 * |
| 李想: "发酵法制备豆渣可溶性膳食纤维", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 洪枫等: "纤维素酶预调控pH值提高木聚糖酶活力的研究", 《南京林业大学学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Verma et al. | Isolation and production of cellulase enzyme from bacteria isolated from agricultural fields in district Hardoi, Uttar Pradesh, India | |
| Zheng et al. | Semi-continuous production of high-activity pectinases by immobilized Rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content | |
| CN102174355B (en) | Concentrated fragrance type distilled spirit pit mud pit-maintaining liquid, production method thereof and concentrated fragrance type distilled spirit pit mud maintenance method | |
| RU2361918C1 (en) | Strain of mycelial mushroom penicillium verruculosum - producer of complex of cellulase, xylanase and xyloglucanase and way of reception of fermental preparation of complex of cellulase, xylanase and xyloglucanase for hydrolysis of cellulose and hemicellulose | |
| CN106834368A (en) | A kind of method that utilization lignocellulose for fermentation produces L lactic acid | |
| Mehboob et al. | Exploring thermophilic cellulolytic enzyme production potential of Aspergillus fumigatus by the solid-state fermentation of wheat straw | |
| Shah et al. | Optimization of cellulase production by Penicillium oxalicum using banana agrowaste as a substrate | |
| Khalil et al. | Production of cellulase by Pleurotus ostreatus and Pleurotus sajor-caju in solid state fermentation of lignocellulosic biomass | |
| CN110713939A (en) | Strain for degrading lignocellulose source inhibitor under extremely low pH condition and application | |
| CN110205349A (en) | A method of bacteria cellulose is prepared using rice bran hydrolyzate fermentation | |
| CN102061270B (en) | Streptomyces thermocarboxydus strain and cultivating method thereof | |
| CN103952447B (en) | Method for producing succinic acid by fermentation under anaerobic condition | |
| CN108541999A (en) | A kind of processing method of anaerobic solid-state fermentation offal and its application | |
| Intasit et al. | Biological pretreatment of empty fruit bunch (EFB) using oleaginous Aspergillus tubingensis TSIP9 | |
| Singh et al. | Evaluation of chemical pre-treatment for biodegradation of agricultural lignocellulosic wastes by Aspergillus niger | |
| CN108795819A (en) | A kind of complex microorganism culture and its application in producing carotenoid | |
| JP5629876B2 (en) | Lactic acid production method by non-sterile fermentation | |
| CN103160543B (en) | Method for improving biogas yield of lignocelluloses-containing raw material | |
| CN102268379A (en) | Trametes trogii and method for producing cellulase by using same | |
| Runajak et al. | Analysis of microbial consortia with high cellulolytic activities for cassava pulp degradation | |
| Zhang et al. | Two-stage co-hydrolysis of rice straw by Trichoderma reesei ZM4-F3 and Pseudomonas aeruginosa BSZ-07 | |
| JP2014176351A (en) | Method for producing ethanol | |
| CN104087568A (en) | Preparation and application of hemicellulase for pulping papermaking bleaching pretreatment | |
| CN103614299A (en) | Mucor circinelloides, method for preparing viscosity-reduction enzyme and application of viscosity-reduction enzyme | |
| CN102250857A (en) | Liquid fermentation technology capable to improve per unit of cellulose activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141008 |
|
| RJ01 | Rejection of invention patent application after publication |