CN104085983A - Method for activating anaerobic ammonia oxidation sludge under low-temperature condition by using ultrasonic waves - Google Patents

Method for activating anaerobic ammonia oxidation sludge under low-temperature condition by using ultrasonic waves Download PDF

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Publication number
CN104085983A
CN104085983A CN201410264993.3A CN201410264993A CN104085983A CN 104085983 A CN104085983 A CN 104085983A CN 201410264993 A CN201410264993 A CN 201410264993A CN 104085983 A CN104085983 A CN 104085983A
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anaerobic ammonia
ammonia oxidation
temperature
temperature condition
ultrasonic
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金仁村
郭琼
俞津津
陈辉
姬玉欣
张正哲
王慧中
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Hangzhou Normal University
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Hangzhou Normal University
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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention provides a method for activating anaerobic ammonia oxidation sludge under a low-temperature condition by using ultrasonic waves. The low-temperature condition indicates that the running temperature of an anaerobic ammonia oxidation reactor is below 30 DEG C. The method comprises the following steps: adding the anaerobic ammonia oxidation sludge to be activated into a reaction container, and performing water bath ultrasonic treatment, wherein the water bath temperature is 31-35 DEG C, and the ultrasonic conditions are that the frequency is 20 kHz, the intensity is 0.7-0.9w/cm<2>, and the time is 1.7-2.0 minutes; and after the ultrasonic treatment, obtaining the activated anaerobic ammonia oxidation sludge. By adopting the method for applying ultrasonic waves to the anaerobic ammonia oxidation sludge, the denitrification performance of anaerobic ammonium oxidation bacteria with poor denitrification performance at the low-temperature condition can be improved; and as proved by testing, the method has the advantages of easiness in operation, quick response, wide application range and the like.

Description

A kind of method of utilizing Anammox mud under ultrasonic activation cold condition
Technical field
The present invention relates to a kind of method of utilizing Anammox mud under ultrasonic activation cold condition.
Background technology
Anammox is a kind of Process of Biological Nitrogen Removal, can under anaerobic taking nitrite as electron acceptor(EA), ammonia nitrogen be oxidized to nitrogen, when realizing two kinds of nitrogen pollutants, remove, than traditional biological denitrification process, Anammox has that denitrification effect is good, energy consumption is low, without the advantage such as additional organic carbon source, running cost be low, prospects for commercial application is wide.But anaerobic ammonia oxidizing bacteria is to envrionment conditions sensitivity, and optimum temperuture is 30～35 DEG C, the too low meeting of temperature causes its nitrogen removal performance to decline.But due to the difference in seasonal variation or geographical position, cause actual operating temperature to be difficult to maintain 30～35 DEG C, and artificial temperature control facility complexity and Financial cost are higher.Maintaining the stable and efficient nitrogen removal performance of anaerobic ammonia oxidizing bacteria is that this process application is in the important prerequisite of actual waste water processing.The method of common raising Anammox nitrogen removal performance mainly contains promotion granular sludge, adds carrier and add nutritive substance etc.Although these technology can improve anaerobic ammonia oxidizing bacteria nitrogen removal performance to a certain extent, all there is certain drawback in these methods, as onset time is long, operating process is complicated, operative technique requires high.
Suitably the ultrasonic radiation of intensity has significant stimulation effect to microbial reaction, can make cell walls and cytolemma attenuation, promote mass transfer, more substrate molecule is entered in cell, increase the contact of enzyme-to-substrate, be conducive to the discharge of meta-bolites simultaneously, accelerate bacterial metabolism and the processing to pollutent.The present invention is used for ultrasonic radiation to activate the activity of anaerobic ammonia oxidizing bacteria, and then improves its nitrogen removal performance, to maintain anaerobic ammonia oxidation process stable and high effective operation under cold condition.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing Anammox mud under ultrasonic activation cold condition, can improve anaerobic ammonia oxidizing bacteria nitrogen removal performance.
The present invention adopts technical scheme to be:
A kind of method of utilizing Anammox mud under ultrasonic activation cold condition, described cold condition refers to that the temperature of anaerobic ammonia oxidation reactor operation is 30 DEG C following (preferably 15～30 DEG C), described method is: in reaction vessel, add Anammox mud to be activated, then carry out water-bath supersound process, bath temperature is 31～35 DEG C, and ultrasound condition is: frequency 20kHz, intensity 0.7～0.9w/cm 2, the time is 1.7～2.0min; After supersound process, obtain the Anammox mud of activation.
Described Anammox mud to be activated is generally the Anammox mud that affected by cold condition to cause nitrogen removal performance to reduce.In the anaerobic ammonia oxidation reactor that the Anammox mud of activation can be used for (being generally 15～30 DEG C of temperature) under cold condition, move.
The Anammox mud of the activation that obtains can add anaerobic ammonia oxidation reactor relaying reforwarding row, and every 7～8 days, the activity of Anammox mud declined, and nitrogen removal performance can repeat above-mentioned activation step after reducing.
Further, the condition optimization of water-bath supersound process is: bath temperature is 32 DEG C, and ultrasound intensity is 0.9w cm -2, ultrasonic time is 1.9min.
The present invention is by causing the anaerobic ammonium oxidation granular sludge that nitrogen removal performance is lower to carry out Ultrasonic Radiation processing on affected by cold condition, can solve rapidly in time the problem that anaerobic ammonia oxidizing bacteria denitrification ability that low temperature causes declines.Evidence this method can improve the nitrogen removal performance of (at 15～30 DEG C of temperature) anaerobic ammonia oxidizing bacteria under cold condition well.
The invention provides the Anammox mud that one can be used for the activation moving in the anaerobic ammonia oxidation reactor of (15～30 DEG C of temperature) under cold condition.
The advantage of patent of the present invention is mainly reflected in: 1) working method is simple, and parameter is easy to control; 2) instant effect; 3) activation effect is good.
Brief description of the drawings
The total nitrogen load (NLR) of Fig. 1 ultrasonic radiation to reactor under differing temps and the influence curve figure of total nitrogen removal load (NRR).In Fig. 1, ◆: R unLR, ◇: R unRR, ■: R cnLR,: R cnRR, ●: the temperature of operating period.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this.
Instrument: rated frequency 28kHz ultrasonic apparatus (Hua Shen section work, Shenzhen), ultrasonic apparatus power is 0-800W.
Embodiment 1:
Step:
1) operation conditions of reactor before sludge activation
Two groups of configurations are identical, the UASB reactor that effective volume is 1.0L, and seed sludge is the anaerobic ammonium oxidation granular sludge of laboratory maturation, seed sludge VSS (volatile suspended substance) is 8.20g L -1, being placed in (without temperature control facility) in laboratory simultaneously, real time record reactor temperature changes, and controlling water inlet pH value is 8.0 ± 0.5, reactor is divided into two groups: test group (R u) and control group (R c), process nitrogenous effluent.
Consisting of of nitrogenous simulated wastewater: (NH 4) 2sO 4210mg L -1, NaNO 2210mg L -1, KH 2pO 410mg L -1, CaCl 22H 2o5.6mg L -1, MgSO 47H 2o300mg L -1, KHCO 31250mg L -1, microelement concentrate I, the each 1.25ml L of II -1.Wherein, microelement concentrate I consist of EDTA5g L -1, FeSO 47H 2o9.14g L -1; And microelement concentrate II consists of EDTA15g L -1, ZnSO 47H 2o0.43gL -1, CoCl 26H 2o0.24g L -1, MnCl 24H 2o0.99g L -1, CuSO 45H 2o0.25gL -1, NaMoO 42H 2o0.22g L -1, NiCl 26H 2o0.21g L -1, H 3bO 30.014g L -1.Solvent is water.
Reactor temperature is from autumn to continuous decrease in winter, and nitric efficiency also reduces gradually, and temperature of reactor is between 22～30 DEG C time, and two reactor total nitrogens are removed load (NRR) and are respectively 4.38kg m -3d -1with 4.78kg m -3d -1, temperature is during lower than 22 DEG C, and two reactor operating performancies significantly worsen.
2) utilize Ultrasonic Radiation activation R uanammox mud in reactor
1. take out R uwhole Anammox mud in reactor, and be collected in the beaker that volume is 1L; 2. the beaker that mud is housed is fixed on to ultrasonic apparatus central authorities; 3. controlling bath temperature is 32 DEG C, and ultrasound intensity is 0.7w cm -2, ultrasonic time is 1.9min; 4. the Anammox mud through Ultrasonic Radiation is put back to R ureactor; 5. maintain former have ready conditions constant, continuous service reactor; 6. after the 8d of interval, repeat 1. above-mentioned～5. operation.
3) R uand R cthe comparative analysis of running condition
The impact that Fig. 1 is ultrasonic radiation on reactor operation usefulness and nitrogen removal performance, represents with total nitrogen load (NLR) and NRR.In Fig. 1 the 8th day starts R uanammox mud in reactor carries out supersound process for the first time, R cmud in reactor does not process.
From figure, can obviously find out R ugroup, after supersound process, has higher operating load, i.e. stronger denitrification ability.Taking reactor temperature during as 15 DEG C as example, R unRR be 5.49kg TN m -3d -1, and that control group is only 1.53kg TN m -3d -1.
Test also finds, through after Ultrasonic Radiation for the first time, and R uin reactor, mud TTC-dehydrogenase activity is 0.58mg TF L -1h -1, far above the 0.08mg TF L of control group -1h -1, present higher biological activity.R simultaneously ugroup VSS can reach 10.11g L -1, the 8.20g L during than inoculation -1have a distinct increment.
Described in this specification sheets embodiment, content is only enumerating of way of realization to inventive concept; protection scope of the present invention should not be regarded as only limiting to the specific form that embodiment states, protection scope of the present invention also and conceive thinkable equivalent technologies means according to the present invention in those skilled in the art.

Claims (4)

1. one kind is utilized the method for Anammox mud under ultrasonic activation cold condition, described cold condition refers to that the temperature of anaerobic ammonia oxidation reactor operation is below 30 DEG C, it is characterized in that described method is: in reaction vessel, add Anammox mud to be activated, then carry out water-bath supersound process, bath temperature is 31～35 DEG C, and ultrasound condition is: frequency 20kHz, intensity 0.7～0.9w/cm 2, the time is 1.7～2.0min; After supersound process, obtain the Anammox mud of activation.
2. the method for claim 1, is characterized in that the condition of described water-bath supersound process is: bath temperature is 32 DEG C, and ultrasound intensity is 0.9w cm -2, ultrasonic time is 1.9min.
3. the method for claim 1, is characterized in that cold condition refers to that the temperature of anaerobic ammonia oxidation reactor operation is 15～30 DEG C.
4. the method for claim 1, is characterized in that the Anammox mud of obtain activation adds anaerobic ammonia oxidation reactor relaying reforwarding row, every 7～8 days, repeats ultrasonic activation step.
CN201410264993.3A 2014-06-13 2014-06-13 Method for activating anaerobic ammonia oxidation sludge under low-temperature condition by using ultrasonic waves Pending CN104085983A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104926055A (en) * 2015-06-04 2015-09-23 江苏大学 Method for increasing production of methane by fermentation of activated sludge

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2007125490A (en) * 2005-11-02 2007-05-24 National Institute Of Advanced Industrial & Technology Anaerobic ammonia treatment method
CN101525179A (en) * 2009-04-20 2009-09-09 广东省微生物研究所 Method for anaerobic ammonia oxidation treatment of low-concentration ammonia nitrogen wastewater
CN103755109A (en) * 2013-12-29 2014-04-30 杭州师范大学 Storage and reactivation methods of anaerobic ammonia oxidation granule sludge

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007125490A (en) * 2005-11-02 2007-05-24 National Institute Of Advanced Industrial & Technology Anaerobic ammonia treatment method
CN101525179A (en) * 2009-04-20 2009-09-09 广东省微生物研究所 Method for anaerobic ammonia oxidation treatment of low-concentration ammonia nitrogen wastewater
CN103755109A (en) * 2013-12-29 2014-04-30 杭州师范大学 Storage and reactivation methods of anaerobic ammonia oxidation granule sludge

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104926055A (en) * 2015-06-04 2015-09-23 江苏大学 Method for increasing production of methane by fermentation of activated sludge

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