CN103693749A - Method for improving denitrification performance of anaerobic ammonium oxidation bacteria by using tourmaline - Google Patents

Method for improving denitrification performance of anaerobic ammonium oxidation bacteria by using tourmaline Download PDF

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CN103693749A
CN103693749A CN201310691077.3A CN201310691077A CN103693749A CN 103693749 A CN103693749 A CN 103693749A CN 201310691077 A CN201310691077 A CN 201310691077A CN 103693749 A CN103693749 A CN 103693749A
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tourmalinite
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张捍民
李义菲
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Dalian University of Technology
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Dalian University of Technology
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Abstract

The invention discloses a method for improving the denitrification performance of anaerobic ammonium oxidation bacteria by using tourmaline, belonging to the technical field of environment engineering. According to the technical scheme, tourmaline powder is added into a reactor filled with anaerobic ammonia oxidation sludge, so that the growth metabolisms of anaerobic ammonium oxidation bacteria are irritated, the pH value and the oxidation reduction potential are effectively regulated, the reaction activity and environment adaptive capacity of the anaerobic ammonium oxidation bacteria are improved, and the high-ammonia nitrogen wastewater is treated. The method has the beneficial effects of greatly improving the activity of the anaerobic ammonium oxidation bacteria and favorably removing total nitrogen; raw materials are easily available, low in cost, capable of being recycled and free of secondary pollution; the method is simple and convenient to operate and beneficial to the reduction of anaerobic ammonium oxidation process investment and operation cost as well as the promotion of popularization of the process.

Description

A kind of method of utilizing tourmalinite to improve anaerobic ammonia oxidizing bacteria nitrogen removal performance
Technical field
The invention belongs to field of environment engineering technology, relate to the new bio denitride technology in sewage disposal, particularly a kind of method of utilizing tourmalinite to improve anaerobic ammonia oxidizing bacteria nitrogen removal performance.
Background technology
In recent years, along with China's Economic development and urbanization process are accelerated, a large amount of nitrogenous compounds drains in water body environment, and polluted by nitrogen situation is on the rise, caused China's many waters body eutrophication, water area ecological environment and human health have been caused to very large harm.In order to control water body polluted by nitrogen, sewage drainage standard has improved in China, and using nitrogen concentration priority control index in sewage disposal during " 12 ".Traditional biological denitrification process is because energy consumption is high, floor space is large, nitric efficiency is low, need the deficiencies such as additional organic carbon source cannot meet high-level sewage water denitrification demand, and the development and application of Novel sewage denitrification process becomes the study hotspot in water environment protection field.
Anammox is a kind of Process of Biological Nitrogen Removal of efficient economy, and Anammox reaction is usingd nitrous acid nitrogen as electron acceptor(EA), and ammonia nitrogen is electron donor, completes autotrophic denitrification.This technique have nitric efficiency high, can reduce by 63% oxygen depletion, reduce release of carbonate dioxide, without advantages such as organic electronic donor, sludge yield are low, be applicable to processing high ammonia-nitrogen wastewater, especially there is unique advantage processing aspect low ratio of carbon to ammonium waste water.Yet Anammox bacteria growing is extremely slow, reach 11 days its generation time, and required growth metabolism condition is very strict, therefore restricted the through engineering approaches application of this technique.
For overcoming the above problems, some are studied by providing external energy field to improve anaerobic ammonia oxidizing bacteria activity, for example magnetic field, electric field and ultrasonic.It is active that this research department adopts the methods such as extra electric field, magnetic field successfully to improve Anammox flora, people such as Liu Sitong 2008 report that at J.Biotechnol. the 138th volume 96-102 page maximum nitrogen removal efficiency improves that 30%, Liu Sitong is bright at Dalian University of Technology's Ph D dissertation the 250th page table in 2009 at electrode voltage is-0.05V under the continuous the action of a magnetic field of 60.0mT sECcontinuous electric field effect under maximum nitrogen removal efficiency improve 20%.People such as this school Duan Xiumei 2011 have studied the effect to Anammox flora activity of low-intensity ultrasonic that frequency is 25kHz at Bioresour.Technol. the 102nd volume 4290-4293 page, and result shows that in ultrasonic density be 0.3w cm -2, action time, while being 4min, nitrogen removal rate improved 25.5%.The people such as this advance sheet Jing Xin report and use a kind of novel anaerobic ammonia oxidation reactor with the ferroelectric utmost point improve Anammox flora activity and accelerate start-up course at Bioresour.Technol. the 114th volume 102-108 page for 2012, through operation in 125 days, there is the nitrogen removal rate (1209.6mg N/L/d) of the reactor of the ferroelectric utmost point apparently higher than control reactor (973.3mg N/L/d).But above method exists larger extra energy consumption and raw materials cost, therefore by method easier and with low cost, improving anaerobic ammonia oxidizing bacteria activity, to realize efficient denitrification significant to biological wastewater treatment.
Summary of the invention
The object of this invention is to provide a kind of method of utilizing tourmalinite to improve anaerobic ammonia oxidizing bacteria nitrogen removal performance.The method can not only effectively improve the reactive behavior of anaerobic ammonia oxidizing bacteria, the pH of adjusting ambient and redox potential, and Promote cell's growth metabolism, and be conducive to the removal of total nitrogen.The method raw material is easily obtained, with low cost, easy and simple to handle, successful, by being conducive to reduce investment and the running cost of anaerobic ammonia oxidation process, promotes popularizing of this technique.
Technical scheme of the present invention is as follows:
Utilize tourmalinite to improve a method for anaerobic ammonia oxidizing bacteria nitrogen removal performance, concrete steps are as follows:
Step 1: the cultivation of anaerobic ammonia oxidizing bacteria: adopt stirring-type Continuous Flow membrane bioreactor to cultivate anaerobic ammonia oxidizing bacteria.Step 2: the pre-treatment of tourmalinite: selected tourmalinite is schorl, makes tourmaline powder through pulverizing, grind, drying, and cleans standby.
Step 3: the treatment process that improves nitrogen removal performance
(1) from reactor, get Anammox mud, flushing, precipitation, centrifugal rear removal upper strata suspended substance, pack above-mentioned Anammox mud in reaction flask into.
(2) synthetic sewage consists of: nitrogenous source is from NH 4 +middle N and NO 2 -middle N, wherein NH 4 +the concentration of middle N is 30-70mg/L, NO 2 -the concentration of middle N is 40-100mg/L;
KHCO 3concentration be 1.25g/L;
KH 2pO 4concentration be 0.025g/L;
CaCl 2.2H 2the concentration of O is 0.3g/L;
MgSO 4.7H 2the concentration of O is 0.2g/L;
FeSO 4concentration be 0.00625g/L;
Liquid microelement is 1.25mL/L;
Liquid microelement composition is: the concentration of EDTA is 15g/L; ZnSO 4.7H 2the concentration of O is 0.43g/L; CoCl 2.6H 2the concentration of O is 0.24g/L; MnCl 24H 2the concentration of O is 0.99g/L; CuSO 4.5H 2the concentration of O is 0.25g/L; NaMoO 4.2H 2the concentration of O is 0.22g/L; NiCl 2.2H 2the concentration of O is 0.19g/L; NaSeO 4.10H 2the concentration of O is 0.21g/L; H 3bO 4concentration be 0.014g/L and NaWO 4.2H 2the concentration of O is 0.050g/L; To synthesize sewage joins in above-mentioned reaction flask; In the Anammox mud obtaining, the concentration of microbial biomass is 1gVSS/L.
(3) add tourmalinite: the tourmaline powder in above-mentioned steps two is joined in above-mentioned reaction flask, and it is 10-400g (gVSSL) that tourmalinite adds concentration range -1, it is 2.5 μ m (d (0.1): 0.916 that tourmalinite adds particle size range; D (0.5): 2.250; D (0.9): 4.429)-62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).(4) regulate pH to 5-11, the nitrogen that exposes to the sun, sealing, in approximately 35 ℃ of shaking tables of constant temperature, cultivates under lucifuge condition.
Effect of the present invention and benefit are:
Tourmalinite material is cheap and easy to get, 200 order-1250 order tourmalinite prices be about 4.5-10 unit/kilogram, its material cost is far below equipment and the operation cost of other external energy fields; Tourmaline capable increases substantially anaerobic ammonia oxidizing bacteria activity, and is conducive to the removal of total nitrogen.Tourmaline capable stimulates the growth metabolism of anaerobic ammonia oxidizing bacteria, and effectively regulates pH and redox potential, improves the environmental compatibility of anaerobic ammonia oxidizing bacteria; Tourmalinite has higher physical and chemical stability, can reuse, and does not produce secondary pollution, and therefore a material drops into and can realize active raising, has avoided follow-up continuous cost; This technological operation is easy, can avoid equipment control and maintenance cost in additive method completely.The present invention improves method that anaerobic ammonia oxidizing bacteria activity processes for high ammonia-nitrogen wastewater and is with a wide range of applications and high application and popularization value by adding tourmalinite, as: the start time that shortens anaerobic ammonia oxidation process, regulate the strict life condition of anaerobic ammonia oxidizing bacteria, and then improve the denitrification effect of this technique.
Accompanying drawing explanation
Fig. 1 is tourmaline powder Sample Scan electron microscope (SEM) imaging.
Fig. 2 adds tourmalinite sample and blank sample anaerobic ammoxidation activity (SAA) temporal evolution situation.
Fig. 3 adds tourmalinite sample and blank sample ammonia nitrogen, nitrous acid nitrogen, the concentration of nitrate nitrogen temporal evolution situation.
Fig. 4 adds tourmalinite sample and blank sample polysaccharide, protein and the contrast of extracellular polymeric (EPS) ultimate production.
Fig. 5 adds tourmalinite sample and blank sample redox potential (ORP) temporal evolution situation.
Fig. 6 adds tourmalinite sample and blank sample pH value temporal evolution situation.
Embodiment
Below in conjunction with technical scheme and accompanying drawing, describe the specific embodiment of the invention in detail.
Embodiment 1
1. the cultivation of anaerobic ammonia oxidizing bacteria:
(1) adopt the laboratory scale Continuous Flow stirring-type membrane bioreactor cultivation anaerobic ammonia oxidizing bacteria of operation continuously, Anammox substratum is composed as follows: NH 4the concentration of Cl is 100mg-N/mL; NaNO 2concentration be 132mg-N/mL; KHCO 3concentration be 1.25g/L; KH 2pO 4concentration be 0.025g/L; CaCl 2.2H 2the concentration of O is 0.3g/L; MgSO 4.7H 2the concentration of O is 0.2g/L; FeSO 4concentration be 0.00625g/L; Trace element: 1.25mL/L waste water; Liquid microelement composition is: the concentration of EDTA is 15g/L; ZnSO 4.7H 2the concentration 0.43g/L of O; CoCl 2.6H 2the concentration of O is 0.24g/L; MnCl 2.4H 2the concentration of O is 0.99g/L; CuSO 4.5H 2the concentration of O is 0.25g/L; NaMoO 4.2H 2the concentration of O is 0.22g/L; NiCl 2.2H 2the concentration of O is 0.19g/L; NaSeO 4.10H 2the concentration of O is 0.21g/L; H 3bO 4concentration be 0.014g/L and NaWO 4.2H 2the concentration of O is 0.050g/L.
(2) with fluorescence in-situ hybridization method, thalline purity is analyzed and drawn: anaerobic ammonia oxidizing bacteria accounts for the more than 90% of whole thalline, wherein " Kuenenia stuttgartiensis " bacterium accounts for the more than 70% of Anammox thalline.Be that " Kuenenia stuttgartiensis " microspecies in anaerobic ammonia oxidizing bacteria are the main body bacterial classifications in reactor.Cultivate suitable time to observing stable nitrogen removal performance, from reactor, get a certain amount of anaerobic ammonia oxidizing bacteria as the function bacterial classification in high ammonia-nitrogen wastewater treatment process.
2. the selection of tourmalinite:
(1) tourmalinite used, from Eastern Inner Mongolia, belongs to schorl, through pulverizing, grind, drying, makes tourmaline powder.Its chemical constitution (wt.%) is: NaO 2: 1.19; MgO:8.05; Cr 2o 3: 0.22; SiO 2: 37.05; K 2o:0.09; CaO:1.18; TiO 2: 0.44; B 2o 3: 10.16; FeO:8.33; Al 2o 3: 9.8.
(2) with laser particle analyzer, measuring tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).By sem observation, tourmalinite is fibrous or cylindrical particle, take long and short post as main, and particle is interdependent arrangement each other, and forms cluster.
(3) with pure water, clean tourmaline powder to remove surface impurity, standby.
3. the treatment process of ammonia nitrogen waste water
(1) from reactor, take out 10mL Anammox mud, after precipitation, remove upper strata suspended substance.With phosphate buffer solution, rinse, centrifugal 5min under 3500rpm condition, rinses and centrifugation step repeats 3 times again.
(2) mud is packed into 100mL serum bottle, add synthetic sewage, in bottle, the mixture cumulative volume of mud and synthetic sewage is 50mL, and gained anaerobic ammonia oxidation microbiological amount concentration is 1gVSS/L.Synthetic sewage consists of: NH 4cl and NaNO 2make nitrogenous source, nitrogenous source compares NH according to the metering of Anammox reactive chemistry 4 +-N:NO 2 --N is that 1:1.32 adds, and is respectively NH 4 +middle N concentration is 50mg/L and NO 2 -middle N concentration is 66mg/L.Other consist of KHCO 3concentration be 1.25g/L; KH 2pO 4concentration be 0.025g/L; CaCl 2.2H 2the concentration of O is 0.3g/L; MgSO 4.7H 2the concentration of O is 0.2g/L; FeSO 4concentration be 0.00625g/L; The concentration of EDTA is 0.00625g/L; Trace element: 1.25mL/L.Liquid microelement composition is: the concentration of EDTA is 15g/L; ZnSO 4.7H 2the concentration of O is 0.43g/L; CoCl 2.6H 2the concentration of O is 0.24g/L; MnCl 2.4H 2the concentration of O is 0.99g/L; CuSO 4.5H 2the concentration of O is 0.25g/L; NaMoO 4.2H 2the concentration of O is 0.22g/L; NiCl 2.2H 2the concentration of O is 0.19g/L; NaSeO 4.10H 2the concentration of O is 0.21g/L; H 3bO 4concentration be 0.014g/L and NaWO 4.2H 2the concentration of O is 0.050g/L.
(3) add tourmalinite: tourmaline powder added to culturing bottle and it is dispersed in mud, adding concentration and be made as 200g (gVSSL) -1, calculating and add 10g tourmaline powder, blank sample does not add tourmalinite.
(4) with the HCl of 0.5M and the NaOH of 0.5M, regulate pH to 8.0 ± 0.1, the nitrogen 1 hour of exposing to the sun is in order to remove dissolved oxygen, and bottle seals with plug, in 35 ℃ of constant temperature, 150rpm shaking table, cultivates 48 hours under lucifuge condition.
4. technological method effect measuring
(1) in culturing process, interval certain hour is measured ammonia nitrogen, nitrous acid nitrogen, the concentration of nitrate nitrogen and pH value,
On-line monitoring redox potential, cultivates and finishes rear working sample extracellular polymeric output (EPS) continuously.With anaerobic ammoxidation activity SAA, evaluate nitrogen removal performance.
SAA = ( NH 4 + - N + NO 2 - - N ) mgL - 1 × 24 gd - 1 1 gVSS · L - 1 × h × 1000 mgg - 1 , G (NH 4 +-N+NO 2 --N) g -1vSSd -1h be incubation time (hour), NH 4 +-N, NO 2 --N is NH 4 +-N and NO 2 -the consumption of-N.
(2) after testing
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 98.3% and 99.9%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 57.7% of blank.The concentration of nitrate nitrogen (15.1mg-N/L) that adds tourmalinite sample is starkly lower than blank (58.9mg-N/L), illustrates that adding tourmalinite not only has obvious promoter action to anaerobic ammoxidation activity, and is conducive to the removal of total nitrogen.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 17.3%, 356.2% and 179.7% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.008-8.155, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Fig. 1 is that in embodiment 1, particle diameter is tourmaline powder Sample Scan electron microscope (SEM) imaging of 62 μ m, and tourmalinite is fibrous or cylindrical particle, take long and short post as main, and particle is interdependent arrangement each other, and forms cluster.
Fig. 2 adds 62 μ m, 200g (gVSSL) in embodiment 1 -1tourmalinite sample and blank sample anaerobic ammoxidation activity (SAA) temporal evolution situation, result shows to cultivate while finishing, and adds tourmalinite sample than the active raising 57.7% of blank.
Fig. 3 adds 62 μ m, 200g (gVSSL) in embodiment 1 -1tourmalinite sample and blank sample ammonia nitrogen, nitrous acid nitrogen, the concentration of nitrate nitrogen temporal evolution situation, result shows to add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 98.3% and 99.9%, does not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and is respectively 63.0% and 62.8%.
Fig. 4 adds 62 μ m, 200g (gVSSL) in embodiment 1 -1tourmalinite sample and blank sample polysaccharide, protein and the contrast of extracellular polymeric (EPS) ultimate production, polysaccharide, protein and extracellular polymeric ultimate production that result shows to add tourmalinite sample increase by 17.3%, 356.2% and 179.7% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
Embodiment 2
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 10g (gVSSL)-1, calculates and adds 0.5g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 92.2% and 99.7%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 53.3% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 0%, 24.8% and 11.9% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 7.998-7.838, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 3
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 8.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 20g (gVSSL)-1, calculates and adds 1g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 97.2% and 99.9%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 56.9% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 0%, 40.9% and 19.6% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.001-8.016, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 4
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL)-1, calculates and adds 2g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 96.6% and 100%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 56.6% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 0%, 187.6% and 89.9% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.033-7.807, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Fig. 5 adds 62 μ m, 40g (gVSSL) in embodiment 4 -1tourmalinite sample and blank sample redox potential (ORP) temporal evolution situation, result shows that tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 5
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 100g (gVSSL)-1, calculates and adds 5g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 96.1% and 100%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 56.2% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 16.1%, 203.8% and 107.7% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 7.999-8.038, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Fig. 6 adds 62 μ m, 100g (gVSSL) in embodiment 5 -1tourmalinite sample and blank sample pH value temporal evolution situation, result show tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 6
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 400g (gVSSL) -1, calculate and add 20g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 96.1% and 99.9%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 56.2% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 15.4%, 260.0% and 132.6% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.042-8.114, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 7
Treatment process condition is:
1. tourmalinite particle diameter is 18 μ m (d (0.1): 3.436; D (0.5): 16.183; D (0.9): 35.326).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 94.4% and 99.9%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 55.0% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 1.9%, 65.7% and 32.5% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.002-8.130, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 8
Treatment process condition is:
1. tourmalinite particle diameter is 12 μ m (d (0.1): 1.732; D (0.5): 10.843; D (0.9): 25.361).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 94.4% and 99.9%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample and improve 40.5% than blank blank is active.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 0%, 151.4% and 72.6% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 7.998-8.153, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 9
Treatment process condition is:
1. tourmalinite particle diameter is 4 μ m (d (0.1): 064; D (0.5): 3.885; D (0.9): 7.590).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 92.2% and 99.8%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 53.4% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 0%, 334.3% and 160.2% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.070-8.173, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 10
Treatment process condition is:
1. tourmalinite particle diameter is 2.5 μ m (d (0.1): 0.916; D (0.5): 2.250; D (0.9): 4.429).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 8.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1. after cultivating and finishing, add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency is respectively 92.2% and 99.8%, do not add tourmalinite sample ammonia nitrogen and nitrous acid nitrogen removal efficiency and be respectively 63.0% and 62.8%.Cultivate while finishing, add tourmalinite sample than the active raising 53.4% of blank.
2. the polysaccharide, protein and the extracellular polymeric ultimate production that add tourmalinite sample increase by 1.9%, 233.3% and 112.8% than blank respectively, illustrate that tourmaline capable promotes the growth metabolism of cell.
3. adding tourmalinite sample pH value variation range is 8.037-8.145, and not adding tourmalinite sample pH value variation range is 8.021-7.635, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
4. tourmalinite can regulate to a certain extent redox potential and it is kept within the specific limits.
Embodiment 11
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 5.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.011g (NH 4 +-N+NO 2 --N) g -1vSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.009g (NH 4 +-N+NO 2 --N) g -1vSSd -1, add tourmalinite sample than the active raising 17.5% of blank.
2. adding tourmalinite sample pH value variation range is 4.995-6.804, and not adding tourmalinite sample pH value variation range is 4.992-5.084, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 12
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 6.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.013g (NH 4 +-N+NO 2 --N) g -1vSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.011g (NH 4 +-N+NO 2 --N) g -1vSSd -1, add tourmalinite sample than the active raising 18.2% of blank.
2. adding tourmalinite sample pH value variation range is 6.042-7.501, and not adding tourmalinite sample pH value variation range is 5.902-6.598, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 13
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 7.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.015g (NH 4 +-N+NO 2 --N) g -1vSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.012g (NH 4 +-N+NO 2 --N) g -1vSSd -1, add tourmalinite sample than the active raising 25.0% of blank.
2. adding tourmalinite sample pH value variation range is 7.039-7.740, and not adding tourmalinite sample pH value variation range is 7.008-7.525, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 14
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 9.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.020g (NH 4 +-N+NO 2 --N) g -1VSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.012g (NH 4 +-N+NO 2 --N) g -1vSSd -1, add tourmalinite sample than the active raising 66.7% of blank.
2. adding tourmalinite sample pH value variation range is 8.925-8.068, and not adding tourmalinite sample pH value variation range is 8.988-8.127, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 15
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 10.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.008g (NH 4 +-N+NO 2 --N) g -1vSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.
2. adding tourmalinite sample pH value variation range is 9.929-9.210, and not adding tourmalinite sample pH value variation range is 9.977-9.404, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 16
Treatment process condition is:
1. tourmalinite particle diameter is 62 μ m (d (0.1): 34.879; D (0.5): 58.020; D (0.9): 95.904).
2. tourmalinite adds concentration and is made as 40g (gVSSL) -1, calculate and add 2g tourmaline powder.
3. regulating pH is 11.0 ± 0.1.
4. other steps as described in Example 1.
Treatment process effect is:
1., after cultivation finishes, adding tourmalinite sample anaerobic ammoxidation activity is 0.006g (NH 4 +-N+NO 2 --N) g -1vSS d -1, not adding tourmalinite sample anaerobic ammoxidation activity is 0.
2. adding tourmalinite sample pH value variation range is 11.025-10.233, and not adding tourmalinite sample pH value variation range is 10.998-10.288, illustrate tourmaline capable effectively regulate pH make its more approach anaerobic ammonia oxidizing bacteria just when 8.0.
Embodiment 17
NH 4 +middle N concentration is 30mg/L and NO 2 -middle N concentration is 40mg/L, and other conditions are as embodiment 1.Embodiment 18
NH 4 +middle N concentration is 70mg/L and NO 2 -middle N concentration is 100mg/L, and other conditions are as embodiment 1.
To sum up embodiment draws, the optimum operation condition that this technology realizes is: tourmalinite particle diameter is 62 μ m, and adding concentration is 200g (gVSSL) -1, pH is 8 ± 0.1.

Claims (8)

1. utilize tourmalinite to improve a method for anaerobic ammonia oxidizing bacteria nitrogen removal performance, it is characterized in that, comprise that step is as follows:
Step 1: cultivate anaerobic ammonia oxidizing bacteria;
Step 2: through pulverizing, grind, dry, clean, schorl is made to tourmaline powder;
Step 3: the treatment process that improves nitrogen removal performance
(1) from reactor, get Anammox mud, flushing, precipitation, centrifugal rear removal upper strata suspended substance, pack Anammox mud in reaction flask into;
(2) synthetic sewage consists of:
Nitrogenous source is from NH 4 +middle N and NO 2 -middle N, wherein NH 4 +the concentration of middle N is 30-70mg/L, NO 2 -the concentration of middle N is 40-100mg/L;
KHCO 3concentration be 1.25g/L;
KH 2pO 4concentration be 0.025g/L;
CaCl 2.2H 2the concentration of O is 0.3g/L;
MgSO 4.7H 2the concentration of O is 0.2g/L;
FeSO 4concentration be 0.00625g/L;
Liquid microelement is 1.25mL/L;
Liquid microelement composition is: the concentration of EDTA is 15g/L; ZnSO 4.7H 2the concentration of O is 0.43g/L; CoCl 2.6H 2the concentration of O is 0.24g/L; MnCl 24H 2the concentration of O is 0.99g/L; CuSO 4.5H 2the concentration of O is 0.25g/L; NaMoO 4.2H 2the concentration of O is 0.22g/L; NiCl 2.2H 2the concentration of O is 0.19g/L; NaSeO 4.10H 2the concentration of O is 0.21g/L; H 3bO 4concentration be 0.014g/L and NaWO 4.2H 2the concentration of O is 0.050g/L;
To synthesize sewage joins in above-mentioned reaction flask; In the Anammox mud obtaining, the concentration of microbial biomass is 1gVSS/L;
(3) add tourmalinite: the tourmaline powder in above-mentioned steps 2 is joined in the reaction flask that above-mentioned (2) obtain, and it is 10-400g (gVSSL) that tourmalinite adds concentration -1;
(4) regulate pH to 5-11, the nitrogen that exposes to the sun, sealing, cultivates under 35 ℃ of lucifuge conditions of constant temperature.
2. method according to claim 1, is characterized in that, it is 100-200g (gVSSL) that described tourmalinite adds concentration -1.
3. method according to claim 1 and 2, is characterized in that, described pH value is 8.0.
4. method according to claim 1 and 2, is characterized in that, the particle diameter of described tourmaline powder is 20-60 μ m.
5. method according to claim 3, is characterized in that, the particle diameter of described tourmaline powder is 20-60 μ m.
6. according to the method described in claim 1,2 or 5, it is characterized in that, described reaction flask is serum bottle.
7. method according to claim 3, is characterized in that, described reaction flask is serum bottle.
8. method according to claim 4, is characterized in that, described reaction flask is serum bottle.
CN201310691077.3A 2013-12-12 2013-12-12 Method for improving denitrification performance of anaerobic ammonium oxidation bacteria by using tourmaline Pending CN103693749A (en)

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