CN104085975B - The method of chloroperoxidase catalytic degradation sulfadimethoxine - Google Patents
The method of chloroperoxidase catalytic degradation sulfadimethoxine Download PDFInfo
- Publication number
- CN104085975B CN104085975B CN201410282976.2A CN201410282976A CN104085975B CN 104085975 B CN104085975 B CN 104085975B CN 201410282976 A CN201410282976 A CN 201410282976A CN 104085975 B CN104085975 B CN 104085975B
- Authority
- CN
- China
- Prior art keywords
- sulfadimethoxine
- chloroperoxidase
- mixed liquor
- add
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of method of chloroperoxidase catalytic degradation sulfadimethoxine, belong to the processing technology field of water, waste water or sewage, the method is based on the biological enzyme system of degrading, in sulfadimethoxine solution to be degraded, add chloroperoxidase, mix rear pH to 2～4.5 that regulate mixed liquor with phosphate buffer solution, then with H2O2For oxidant, in mixed liquor, add H2O2The aqueous solution, stirring at room temperature degraded sulfadimethoxine. The present invention is simple to operate, chloroperoxidase and H2O2Consumption is few, reaction condition gentleness, and molecular structure that can rapid damage sulfadimethoxine, makes it be fractured into little molecule, reduce its pollution to environment, and degradation time is short, and in 10～20 minutes, sulfadimethoxine degradation rate can reach 80%～96%.
Description
Technical field
The invention belongs to the processing technology field of water, waste water or sewage, be specifically related to one and utilize enzymatic oxidation methodRealize the method for sulfadimethoxine fast degradation.
Background technology
Sulfadimethoxine is common antiseptic, because it has the significantly spy such as anti-infective, efficient, stable in propertiesPoint, is widely used in edible animal as prevention and medicine for treatment. But sulfadimethoxine is difficult to be inhaledReceive, wherein 30%～90% excrete with the form of urine or ight soil. The current municipal sewage in many countries,In the surrounding mediums such as river, underground water and soil, rivers and lakes deposit, all detect sulfadimethoxine. ItsAfter entered environment, can stimulate pathogen to develop immunity to drugs, and can enter human body by food chain, destroy making of peopleBlood system, causes hemolytic anemia disease, affects human health. Find, sulfamethazine may bring out to be nibbledTooth class animal thyroid gland hyperplasia or tumour.
At present, about the processing method of sulfadimethoxine have full cell method, ultrafiltration, Fenton oxidizing process,UV photocatalytic method, O3Oxidizing process and ClO2Oxidizing process etc. Wherein, when full cell method is degraded, use duration (> 12Hour), without specific aim, almost can not remove sulfadimethoxine and pollute; Low (the < of efficiency when ultrafiltration degraded10%); When the degraded of Fenton oxidizing process, can produce secondary pollution; When UV photocatalysis degradation, cost is high and use duration(> 1 hour); O3When oxidizing process degraded, sulfadimethoxine and O3Concentration ratio be 1:8.5, and O3Need online generation, equipment requirement is high; ClO2When oxidizing process degraded, the consumption of oxidant and catalyst is large.
Summary of the invention
Technical problem to be solved by this invention is to overcome the shortcoming of above-mentioned sulfadimethoxine processing method, carriesA kind for the treatment of process of confession is simple, cost is low, efficiency is high, eco-friendly chloroperoxidase catalytic degradation sulfanilamide (SN) twoThe method of Sulfamonomethoxine.
Solving the problems of the technologies described above adopted technical scheme is that it is made up of following step: to sulfanilamide (SN) to be degraded twoIn Sulfamonomethoxine solution, add chloroperoxidase, mix, with the phosphate buffer solution adjusting of 0.1mol/LPH to 2～4.5 of mixed liquor, then adding mass fraction is 30% H2O2The aqueous solution, stirs at ambient temperatureMix described sulfadimethoxine and chloroperoxidase, H 5～20 minutes2O2Mol ratio be 1:2.67 × 10-6 ～6.25×10-5:5.2～17.2。
The preferred sulfadimethoxine of the present invention and chloroperoxidase, H2O2Mol ratio be 1:3.0 × 10-5～6.0×10-5: 10.5～17.2, preferably use the phosphate buffer solution of 0.1mol/L to regulate pH to 2.5～3.5 of mixed liquor.Preferably stir at ambient temperature 10～20 minutes.
The present invention is based on biological enzyme degraded system, with H2O2For oxidant, can its molecule knot of rapid damageStructure is to reach the object of degraded. The method is simple to operate, chloroperoxidase and H2O2Consumption is few, reaction conditionGentleness, environmental protection, degradation time is short, in 10～20 minutes sulfadimethoxine degradation rate can reach 80%～96％。
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in more detail, but protection scope of the present invention is not limited only to these in factExecute example.
Embodiment 1
To the chlorine peroxidating that adds 90 μ L1 μ mol/L in the sulfadimethoxine aqueous solution of 30mL64 μ mol/LThing enzyme solutions (phosphate buffer solution and chloroperoxidase by 0.1mol/L are formulated), mixes, with 0.1The phosphate buffer solution of mol/L regulates the pH to 3 of mixed liquor, then adds the H that 3 μ L mass fractions are 30%2O2WaterSolution, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:4.69 × 10-5: 15.6,Under room temperature condition, stir 15 minutes. By the concentration of sulfadimethoxine before and after the degraded of high-efficient liquid phase color spectrometry, pressFollowing formula calculates the degradation rate of sulfadimethoxine:
η＝(C0-Ct)/C0×100％
In formula, η is degradation rate, C0For the initial concentration of sulfadimethoxine, CtSulfadimethoxine during for time tConcentration. As calculated, the degradation rate of sulfadimethoxine is 95%.
Embodiment 2
In the present embodiment, regulate the pH to 2 of mixed liquor with the phosphate buffer solution of 0.1mol/L, other steps and realityExecute example 1 identical, as calculated, the degradation rate of sulfadimethoxine is 56%.
Embodiment 3
In the present embodiment, regulate the pH to 2.5 of mixed liquor with the phosphate buffer solution of 0.1mol/L, other steps withEmbodiment 1 is identical, and as calculated, the degradation rate of sulfadimethoxine is 82%.
Embodiment 4
In the present embodiment, regulate the pH to 3.5 of mixed liquor with the phosphate buffer solution of 0.1mol/L, other steps withEmbodiment 1 is identical, and as calculated, the degradation rate of sulfadimethoxine is 88%.
Embodiment 5
In the present embodiment, regulate the pH to 4 of mixed liquor with the phosphate buffer solution of 0.1mol/L, other steps and realityExecute example 1 identical, as calculated, the degradation rate of sulfadimethoxine is 76%.
Embodiment 6
In the present embodiment, regulate the pH to 4.5 of mixed liquor with the phosphate buffer solution of 0.1mol/L, other steps withEmbodiment 1 is identical, and as calculated, the degradation rate of sulfadimethoxine is 54%.
Embodiment 7
In the present embodiment, add the H that 1 μ L mass fraction is 30%2O2The aqueous solution, other steps and embodiment 1 phaseWith, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:4.69 × 10-5: 5.2. Through meterCalculate, the degradation rate of sulfadimethoxine is 70%.
Embodiment 8
In the present embodiment, add the H that 2 μ L mass fractions are 30%2O2The aqueous solution, other steps and embodiment 1 phaseWith, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:4.69 × 10-5: 10.5. WarpCalculate, the degradation rate of sulfadimethoxine is 88%.
Embodiment 9
In the present embodiment, add the H that 3.3 μ L mass fractions are 30%2O2The aqueous solution, other steps and embodiment 1Identical, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:4.69 × 10-5:17.2。As calculated, the degradation rate of sulfadimethoxine is 96%.
Embodiment 10
The chloroperoxidase solution that adds 5 μ L1 μ mol/L in the present embodiment, other steps are identical with embodiment 1,Wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:2.67 × 10-6: 15.6. As calculated,The degradation rate of sulfadimethoxine is 62%.
Embodiment 11
In the present embodiment, add the chloroperoxidase solution of 30 μ L1 μ mol/L, other steps and embodiment 1 phaseWith, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:1.56 × 10-5: 15.6. WarpCalculate, the degradation rate of sulfadimethoxine is 77%.
Embodiment 12
In the present embodiment, add the chloroperoxidase solution of 60 μ L1 μ mol/L, other steps and embodiment 1 phaseWith, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:3.13 × 10-5: 15.6. WarpCalculate, the degradation rate of sulfadimethoxine is 91%.
Embodiment 13
In the present embodiment, add the chloroperoxidase solution of 120 μ L1 μ mol/L, other steps and embodiment 1Identical, wherein sulfadimethoxine and chloroperoxidase, H2O2Mol ratio be 1:6.25 × 10-5:15.6。As calculated, the degradation rate of sulfadimethoxine is 96%.
Embodiment 14
In the present embodiment, stir at ambient temperature 5 minutes, other steps are identical with embodiment 1. As calculated, sulfanilamide (SN)The degradation rate of SDM is 61%.
Embodiment 15
In the present embodiment, stir at ambient temperature 10 minutes, other steps are identical with embodiment 1. As calculated, sulphurThe degradation rate of amine SDM is 80%.
Embodiment 16
In the present embodiment, stir at ambient temperature 20 minutes, other steps are identical with embodiment 1. As calculated, sulphurThe degradation rate of amine SDM is 96%.
Claims (1)
1. a method for chloroperoxidase catalytic degradation sulfadimethoxine, is characterized in that: fall to waitingIn the sulfadimethoxine solution of separating, add chloroperoxidase, mix, slow with the phosphoric acid of 0.1mol/LDissolved liquid regulates pH to 2.5～3.5 of mixed liquor, and then adding mass fraction is 30% H2O2The aqueous solution,Under room temperature condition, stir described sulfadimethoxine and chloroperoxidase, H 10～20 minutes2O2RubYou are than being 1:3.0 × 10-5～6.0×10-5:10.5～17.2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410282976.2A CN104085975B (en) | 2014-06-23 | 2014-06-23 | The method of chloroperoxidase catalytic degradation sulfadimethoxine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410282976.2A CN104085975B (en) | 2014-06-23 | 2014-06-23 | The method of chloroperoxidase catalytic degradation sulfadimethoxine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104085975A CN104085975A (en) | 2014-10-08 |
| CN104085975B true CN104085975B (en) | 2016-05-04 |
Family
ID=51633772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410282976.2A Expired - Fee Related CN104085975B (en) | 2014-06-23 | 2014-06-23 | The method of chloroperoxidase catalytic degradation sulfadimethoxine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104085975B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104368121B (en) * | 2014-10-31 | 2017-05-17 | 陕西师范大学 | Method for catalytically converting atrazine by virtue of chloroperoxidase |
| CN104402121B (en) * | 2014-11-20 | 2016-06-15 | 陕西师范大学 | The method of chloroperoxidase catalyzed degradation triazophos |
| CN116425316B (en) * | 2023-03-10 | 2023-11-21 | 中国水产科学研究院黄海水产研究所 | Application of sperm whale Mb and mutant H64D/V68I Mb in degradation of sulfonamide compounds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997017301A1 (en) * | 1995-11-07 | 1997-05-15 | Akzo Nobel N.V. | Process for the degradation of chlorite |
| CN1962477A (en) * | 2006-10-26 | 2007-05-16 | 上海交通大学 | Process for catalyzing oxidation of organic compounds by ultraviolet |
| CN101781012A (en) * | 2009-12-31 | 2010-07-21 | 陕西师范大学 | Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2 |
-
2014
- 2014-06-23 CN CN201410282976.2A patent/CN104085975B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997017301A1 (en) * | 1995-11-07 | 1997-05-15 | Akzo Nobel N.V. | Process for the degradation of chlorite |
| CN1962477A (en) * | 2006-10-26 | 2007-05-16 | 上海交通大学 | Process for catalyzing oxidation of organic compounds by ultraviolet |
| CN101781012A (en) * | 2009-12-31 | 2010-07-21 | 陕西师范大学 | Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104085975A (en) | 2014-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liang et al. | Anaerobic fermentation of waste activated sludge for volatile fatty acid production: recent updates of pretreatment methods and the potential effect of humic and nutrients substances | |
| Zhu et al. | Protection mechanisms of periphytic biofilm to photocatalytic nanoparticle exposure | |
| Vasudevan | An efficient removal of phenol from water by peroxi-electrocoagulation processes | |
| Abelleira et al. | Advanced thermal hydrolysis: optimization of a novel thermochemical process to aid sewage sludge treatment | |
| Zhang et al. | Sewage sludge disintegration by combined treatment of alkaline+ high pressure homogenization | |
| CN104085975B (en) | The method of chloroperoxidase catalytic degradation sulfadimethoxine | |
| Demadis et al. | “Green” scale inhibitors in water treatment processes: the case of silica scale inhibition | |
| Luo et al. | Optimization of Fenton process on removing antibiotic resistance genes from excess sludge by single-factor experiment and response surface methodology | |
| Hadadi et al. | Comparison of four plant-based bio-coagulants performances against alum and ferric chloride in the turbidity improvement of bentonite synthetic water | |
| CN102718295A (en) | Compound medicament for treating coking wastewater and preparation method thereof | |
| CN103864185A (en) | Method and device for high-efficiently and quickly oxidizing and fixing arsenic in wastewater based on glow discharge | |
| Mitraka et al. | A comprehensive review on pretreatment methods for enhanced biogas production from sewage sludge | |
| CN103349001A (en) | Bactericide for cooling water of petrochemical system | |
| CN104085963A (en) | Combined electrochemical ozone treatment device for antibiotic wastewater and treatment method thereof | |
| CN110540283A (en) | Method for enhanced removal of sulfamethoxazole in water | |
| Wu et al. | Cost-effective FeIVO2+ generation for antibiotics removal in electrochlorination of mariculture wastewater | |
| CN109574193B (en) | Ruthenium ruthenate lanthanide perovskite catalyst and method for degrading carbamazepine by heterogeneous activated peroxymonosulfate | |
| CN105084512A (en) | Method for promoting Fe(III)/H2O2 system to repair organic polluted water through biochar synthesized by composting products | |
| CN104649395A (en) | An efficient flocculant | |
| CN104445700A (en) | Treatment and reuse method of guanidine gum fracturing wastewater | |
| CN104692510A (en) | Preparation method for solid polymer ferric sulfate zinc flocculant | |
| CN104098170A (en) | Decoloration method and system for electroplating wastewater | |
| CN104211157A (en) | Method for preparing sustained release potassium ferrate clathrate compound for water treatment | |
| KR20160014144A (en) | Method for treatment of microwave-assisted solubilization efficiency for waste activated sludge by addition of food wastewater | |
| CN102531117A (en) | Composite inorganic water purifying agent and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160504 Termination date: 20200623 |