CN104069188B - Chinese medicine composition of a kind of Cure of depression and preparation method thereof - Google Patents
Chinese medicine composition of a kind of Cure of depression and preparation method thereof Download PDFInfo
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Abstract
The invention provides Chinese medicine composition of a kind of Cure of depression and preparation method thereof.The crude drug of this Chinese medicine composition mainly comprises Herba Epimedii, Radix Polygoni Multiflori Preparata, the Radix Astragali.Described preparation method comprises: get crude drug in proportion, with water or the organic solvent extraction that dissolves each other with water; Or, get crude drug in proportion, adopt decoction and alcohol sedimentation technique preparation.Chinese medicine composition of the present invention has obvious antidepressant effect.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, be specifically related to Chinese medicine composition of a kind of Cure of depression and preparation method thereof, belong to field of medicaments.
Background technology
Depression is a kind of common mental sickness, and its prevalence expects the year two thousand twenty will rise to the 2nd.Opinions vary for its pathogenesis, and scholars think it may is the interactive result of the many factors such as psychosocial factor and various biological modifications.Wherein, psychosocial factor mainly contains nervous work and fast pace life, bad inter personal contact, burst life events strike etc.; Biochemical mechanism aspect mainly comprises monoamine hypothesis, receptors hypothesis, endocrine hypothesis, and immunity changes.Monoamine neurotransmitter hypothesis think depression time, the content of the monoamine neurotransmitter (5-HT, NE, DA) of maincenter reduces.Endocrine hypothesis relates generally to HPA(HPA) axle, stress time, hpa axis is excited, mainly cause CRH, ACTH(corticotropin releasing hormone, thyroliberin) and GC(glucocorticoid) content increase, and increase GC Immunosuppression reaction.
Therefore, the medicine developed for monoamine neurotransmitter becomes the technical problem needing in the industry solution badly.
Summary of the invention
The present invention adopts CUMS depression model rat to screen the Chinese medicine composition product with good Cure of depression effect.
The present invention's first object is the Chinese medicine composition providing a kind of Cure of depression;
The present invention's second object is the preparation method providing this Chinese medicine composition.
The object of the invention is to be achieved through the following technical solutions:
A Chinese medicine composition for Cure of depression, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 8-35 weight portion, Radix Polygoni Multiflori Preparata 20-75 weight portion, Radix Astragali 10-50 weight portion.
Further, its crude drug consists of:
Herba Epimedii 9-30 weight portion, Radix Polygoni Multiflori Preparata 25-70 weight portion, Radix Astragali 12-45 weight portion.
Further, its crude drug consists of:
Herba Epimedii 10-25 weight portion, Radix Polygoni Multiflori Preparata 30-65 weight portion, Radix Astragali 15-35 weight portion.
Further, its crude drug consists of:
Herba Epimedii 10-20 weight portion, Radix Polygoni Multiflori Preparata 35-65 weight portion, Radix Astragali 15-27 weight portion.
Further, its crude drug consists of:
Herba Epimedii 15 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 22 weight portion;
Or, Herba Epimedii 25 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 22 weight portion;
Or, Herba Epimedii 15 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 36 weight portion.
Chinese medicine composition of the present invention can be prepared as follows:
Get crude drug in proportion, with water or the organic solvent extraction that dissolves each other with water;
Further, the described organic solvent dissolved each other with aqueous phase is selected from one or more in methanol, ethanol, acetone; Further be preferably the ethanol of 50-90%; Further be preferably the ethanol of 60-80%.
Chinese medicine composition of the present invention also can be prepared as follows:
Get crude drug in proportion, adopt decoction and alcohol sedimentation technique preparation; Wherein said alcohol precipitation concentration is 50-90%;
Further, described alcohol precipitation concentration is 60-80%.
Chinese medicine composition of the present invention carries out except the method preparation of united extraction except employing is above-mentioned to crude drug, and can also adopt and prepare the method that each crude drug carries out extracting separately respectively, concrete grammar is as follows:
Get Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata medical material in proportion, respectively with water extraction, or with the organic solvent extraction dissolved each other with water, or carry out water extract-alcohol precipitation, prepare Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract, Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract are merged, to obtain final product; The wherein said organic solvent dissolved each other with aqueous phase is selected from one or more in methanol, ethanol, acetone; Described alcohol precipitation concentration is 50-90%;
Further, the described organic solvent dissolved each other with aqueous phase is the ethanol of 50-90%; Further be preferably the ethanol of 60-80%;
Further, described alcohol precipitation concentration is 60-80%;
Further, described Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract can be exquisite further, as adopted punching adsorption resin column.
Extracting method used in above-mentioned preparation method comprises one or more modes decocted in extraction, reflux, extract, soak extraction, supersound extraction or seepage pressure effects.
Further, the preparation method of Chinese medicine composition of the present invention comprises the steps:
A, get epimedium herb, water boiling and extraction, extracting solution adds ethanol precipitate with ethanol, prepares Herba Epimedii extract;
B, get Milkvetch Root, water boiling and extraction, extracting solution is concentrated to obtain Radix Astragali extract;
C, get Radix Polygoni Multiflori Preparata medical material, water boiling and extraction, extracting solution adds ethanol precipitate with ethanol, prepares Radix Polygoni Multiflori extract;
D, get Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract, mixing.
Further, the preparation method of Chinese medicine composition of the present invention comprises the steps:
A, get epimedium herb, water boiling and extraction, it is 60%-80% that extracting solution adds ethanol to alcohol content, filters, and filtrate regulates pH to be alkalescence, filters, and filtrate regulates pH to neutral, reclaims ethanol, obtains Herba Epimedii extract;
B, get Milkvetch Root, water boiling and extraction, extracting solution is concentrated to obtain Radix Astragali extract;
C, get Radix Polygoni Multiflori Preparata medical material, water boiling and extraction, it is 70%-80% that extracting solution adds ethanol to alcohol content, filters, and filtrate regulates pH to be alkalescence, filters, and filtrate regulates pH to neutral, reclaims ethanol, obtains Radix Polygoni Multiflori Preparata extract;
D, get Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract, mixing, to obtain final product.
Further, steps A aqueous extract carries out ethanol precipitation twice, and alcohol precipitation concentration is 65%-70% for the first time, and second time is 75%-80%; Further, alcohol precipitation concentration is 65% for the first time, and second time is 80%.
Further, step C aqueous extract carries out ethanol precipitation twice, and alcohol precipitation concentration is 65%-70% for the first time, and second time is 75%-80%; Further, alcohol precipitation concentration is 70% for the first time, and second time is 80%.
Further, alkalescence described in steps A, C is pH8.0-9.0.
Further, steps A, C carry out precipitate with ethanol and after regulating pH, leave standstill 12-36 hour.
Above-mentioned preparation method Raw medicine also can be purified, refining after extracting, as crossed macroporous resin column; And preparation process routinely makes the acceptable any conventional dosage form of pharmaceutics further, comprises granule, tablet, capsule, drop pill, oral liquid, suspension, emulsion, injection.
For enabling above-mentioned dosage form realize, the acceptable adjuvant of pharmacy need be added when preparing these dosage forms, such as: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises: starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, acetic acid chloroethene are fixed, Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods; Substrate comprises: PEG6000, PEG4000, insect wax etc.For enabling above-mentioned dosage form realize pharmacy of Chinese materia medica, pharmacy other adjuvant acceptable (Fan Biting " pharmacy of Chinese materia medica ", the adjuvant that in Shanghai Science Press December the 1st edition in 1997, each dosage form is recorded) need be added when preparing these dosage forms.
Chinese medicine composition of the present invention is except the form fed intake with Herba Epimedii, Radix Polygoni Multiflori Preparata, Radix Astragali crude drug, the form fed intake with the extract of Herba Epimedii, Radix Polygoni Multiflori Preparata, the Radix Astragali (effective site) can also be adopted, therefore the present invention further discloses a kind of Chinese medicine composition of Cure of depression:
A Chinese medicine composition for Cure of depression, the raw material of this Chinese medicine composition consists of:
Herba Epimedii extract 8-35 weight portion, Radix Polygoni Multiflori Preparata extract, 20-75 weight portion, Radix Astragali extract 10-50 weight portion.
Further, its crude drug consists of:
Herba Epimedii extract 9-30 weight portion, Radix Polygoni Multiflori Preparata extract 25-70 weight portion, Radix Astragali extract 12-45 weight portion.
Further, its crude drug consists of:
Herba Epimedii extract 10-25 weight portion, Radix Polygoni Multiflori Preparata extract 30-65 weight portion, Radix Astragali extract 15-35 weight portion.
Further, its crude drug consists of:
Herba Epimedii extract 10-20 weight portion, Radix Polygoni Multiflori Preparata extract 35-65 weight portion, Radix Astragali extract 15-27 weight portion.
Further, its crude drug consists of:
Herba Epimedii extract 15 weight, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 22 parts;
Or, Herba Epimedii extract 25 weight portion, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 22 weight portion;
Or, Herba Epimedii extract 15 weight portion, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 36 weight portion.
Herba Epimedii extract of the present invention, Radix Polygoni Multiflori Preparata extract, Radix Astragali extract are respectively Herba Epimedii, Radix Polygoni Multiflori Preparata, the water extract of the Radix Astragali or the extractive with organic solvent that dissolves each other with aqueous phase; Or, be respectively Herba Epimedii, extract that Radix Polygoni Multiflori Preparata, the Radix Astragali obtain after water extract-alcohol precipitation process.
Wherein, the described organic solvent dissolved each other with aqueous phase is selected from any one in methanol, ethanol, acetone; More preferably concentration is the ethanol of 50%-90%; Further being preferably concentration is the ethanol of 60%-80%;
Described alcohol precipitation concentration is 50%-90%%; More preferably alcohol precipitation concentration 60%-80%.
Further, described Herba Epimedii extract is adopted and is prepared with the following method: get epimedium herb, water boiling and extraction, it is 60%-80% that extracting solution adds ethanol to alcohol content, filter, filtrate regulates pH to be alkalescence, filters, filtrate regulates pH to neutral, reclaims ethanol, obtains Herba Epimedii extract; Further, extracting solution carries out ethanol precipitation twice, and alcohol precipitation concentration is 65% for the first time, and second time is 80%; Further, described alkalescence is pH8.0-9.0; Further, carry out precipitate with ethanol and after regulating pH, leave standstill 12-36 hour;
Described Radix Polygoni Multiflori Preparata extract is adopted and is prepared with the following method: get Radix Polygoni Multiflori Preparata medical material, water boiling and extraction, it is 70%-80% that extracting solution adds ethanol to alcohol content, filter, filtrate regulates pH to be alkalescence, filters, filtrate regulates pH to neutral, reclaims ethanol, obtains Radix Polygoni Multiflori Preparata extract; Further, extracting solution carries out ethanol precipitation twice, and alcohol precipitation concentration is 70% for the first time, and second time is 80%; Further, described alkalescence is pH8.0-9.0; Further, carry out precipitate with ethanol and after regulating pH, leave standstill 12-36 hour;
" Radix Polygoni Multiflori Preparata " preparation that Radix Polygoni Multiflori Preparata of the present invention records with reference to 2010 editions " Chinese Pharmacopoeias " first.
Decoction and alcohol sedimentation technique of the present invention means in Chinese medicine water extracting liquid, adds the alcohol content (i.e. alcohol precipitation concentration) that ethanol makes to reach regulation, and some composition dissolubility in alcoholic solution reduces separates out precipitation, filter, get alcoholic solution, reclaim ethanol and obtain extract, make the method that Aqueous extracts is refined.
Experimentation shows, Chinese medicine composition of the present invention (CF4) obviously can raise the Open-field scoring of CUMS model mouse, body weight, Thymus and spleen coefficient, the content of Hippocampus 5-HT, NE, DA and the percentage ratio (the equal <0.05 of P) of Hippocampus CA 3 Region neuron population order and normal neurons, obviously can reduce serum 5-HT, NE, DA, ACTH, CORT, β-EP content, adrenal gland's coefficient and adrenal cortex thickness (the equal <0.05 of P).
Accompanying drawing explanation
Fig. 1 rat hippocampal CA 3 neuron, HE dyes, and × 400,
The rat hippocampal CA 3 neuron of Fig. 1-1, normal group, HE dyes, and × 400,
The rat hippocampal CA 3 neuron of Fig. 1-2, model group, HE dyes, and × 400,
The rat hippocampal CA 3 neuron of Fig. 1-3, model+Sertraline group, HE dyes, and × 400,
The rat hippocampal CA 3 neuron of Fig. 1-4, model+HLS group, HE dyes, and × 400,
The rat hippocampal CA 3 neuron of Fig. 1-5, model+CF4 group, HE dyes, and × 400.
Effect experimental examples
Experimental example 1
1 material
The complete male Kunming mouse 260 of 1.1 animal cleaning grade, in 6 ~ 8 week age, weight 24 ~ 29g, Sichuan University's Experimental Animal Center provides, the quality certification: real dynamic No. 10, the Guan Zhi in river, before experiment, Animal adaptability is raised 3 ~ 5 days.Feeding environment, room temperature 20 ~ 25 DEG C, humidity 55 ~ 70%, drinking-water of freely ingesting.
1.2 medicines and reagent
Control drug sertraline hydrochloride sheet (Sertraline) (110801), Dalian pfizer inc, prepared before use becomes the 0.5%CMC-Na suspension of 37.5%, 62.5% concentration.
Experimental agents 1HLS: Radix Polygoni Multiflori Preparata 1000g, Radix Astragali 440g, Rhizoma Polygonati (system) 440g, Herba Epimedii 300g, Fructus Lycii 300g, Radix Salviae Miltiorrhizae 220g;
Preparation method: above Six-element, Radix Polygoni Multiflori Preparata, Radix Salviae Miltiorrhizae, Fructus Lycii decoct with water three times, collecting decoction, filter, filtrate is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 70%, places, and filters, filtrate adds ethanol again makes alcohol content reach 80%, places, and filters, with 10% sodium hydroxide solution adjust ph to 8.0, place, filter, filtrate is by 10% hydrochloric acid solution adjust ph to 7.0, and reclaim ethanol, medicinal liquid is for subsequent use; Herba Epimedii, Rhizoma Polygonati decoct with water secondary, collecting decoction, filter, leave standstill, get supernatant concentration to appropriate, let cool, add ethanol and reach 65% to alcohol content, leave standstill, filter, filtrate adds ethanol makes alcohol content reach 80%, leaves standstill, and filters, filtrate, by 10% sodium hydroxide solution adjust ph to 8.0, leaves standstill, and filters, filtrate is by 10% hydrochloric acid solution adjust ph to 7.0, and reclaim ethanol, medicinal liquid is for subsequent use; The Radix Astragali decocts with water three times, collecting decoction, and filter, filtrate is concentrated into about 95ml, and medicinal liquid is for subsequent use.Merge above medicinal liquid for subsequent use, stir evenly, cold preservation is placed, and filters, adds water to 1000ml, add appropriate Sugarless type sweeting agent, and filter, filtrate, by 10% sodium hydroxide solution adjust ph to 7.0, to obtain final product.
Experimental agents 2CF1: Radix Polygoni Multiflori Preparata 1000g, Fructus Lycii 300g, Rhizoma Polygonati 440g;
Preparation method: get Radix Polygoni Multiflori Preparata 1000g, Fructus Lycii 300g, adds to boil and carries three times, merging filtrate, filter, heating is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 70%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 80%, then leaves standstill 24 hours, filters, filtrate regulates pH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, and reclaim ethanol, medicinal liquid is for subsequent use.Get Rhizoma Polygonati 440g, add 10 times of water gagings, boil and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add an alcohol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate regulates pH value to 8.0 with 10% sodium hydroxide solution, leave standstill 24 hours, filter, filter, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, and reclaim ethanol, medicinal liquid is for subsequent use.Merge above reserve liquid, stir evenly, cold preservation places 12 hours, filters, adds water to 1000ml, filters, and filtrate is by 10% sodium hydroxide adjust ph to 7.0, and heated and boiled sterilizing, to obtain final product.
Experimental agents 3CF2: Herba Epimedii 300g
Preparation method: take epimedium herb 300g, add 10 times of water gagings, boil and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add an alcohol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate is by 10% sodium hydroxide solution adjust ph to 8.0, leave standstill 24 hours, filter, filter, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaim ethanol, filter, add water to 1000ml, filter, filtrate by 10% sodium hydroxide adjust ph to 7.0-7.5, heated and boiled sterilizing, obtain.
Experimental agents 4CF3: epimedium herb 300g, Radix Polygoni Multiflori Preparata 1000g;
Preparation method: take epimedium herb 300g, adds 10 times of water gagings, boils and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add an alcohol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate is by 10% sodium hydroxide solution adjust ph to 8.0, leave standstill 24 hours, filter, filter, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Take 1000g Radix Polygoni Multiflori Preparata, add to boil and carry three times, merging filtrate, filter, heating is concentrated into syrup and strengthens, and lets cool, adding ethanol makes alcohol content reach 70%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 80%, then leaves standstill 24 hours, filters, filtrate regulates PH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Get extraction ofHerba Epimedii, Radix Polygoni Multiflori extracting solution stirs evenly, cold preservation places 12 hours, filters, adds water to 1000ml, filters, and filtrate is by 10% sodium hydroxide adjust ph to 7.0, and heated and boiled sterilizing, to obtain final product.
Experimental agents 5CF4: for embodiment 1 prepares oral liquid.
Experimental agents 6CF5: Radix Polygoni Multiflori Preparata 1000g, Radix Astragali 440g, Herba Epimedii 300g, Radix Salviae Miltiorrhizae 220g;
Preparation method: get Radix Polygoni Multiflori Preparata 1000g, Radix Salviae Miltiorrhizae 220g, adds decocting in water and carries three times, merging filtrate, filter, heating is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 70%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 80%, then leaves standstill 24 hours, filters, filtrate regulates pH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, and reclaim ethanol, medicinal liquid is for subsequent use.Take epimedium herb 300g, add 10 times of water gagings, boil and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add an alcohol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate is by 10% sodium hydroxide solution adjust ph to 8.0, leave standstill 24 hours, filter, filter, filtrate regulates PH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use.Take Radix Astragali 440g, add 10 times amount water extraction three times, each 1.5 hours, merge extractive liquid, filter, concentrated for subsequent use.The mixing of said extracted liquid stirred evenly, cold preservation places 12 hours, filters, adds water to 3000ml, filters, and filtrate is by 10% sodium hydroxide adjust ph to 7.0, and heated and boiled sterilizing, to obtain final product.
" Radix Polygoni Multiflori Preparata " used in above-mentioned Experimental agents and " Rhizoma Polygonati (system) " is respectively the processed product of Radix Polygoni Multiflori and Rhizoma Polygonati, the preparation method of " Radix Polygoni Multiflori Preparata " and " Rhizoma Polygonati processed product " that concrete preparation method is recorded with reference to 2010 editions " Chinese Pharmacopoeias " first.
1.3 key instrument MK3 microplate reader (U.S. Thermo); AllegraX-22RCentrifuge(U.S. Backman); 81m-25 optical microscope (Olympus company); ZZ-6 mice autonomic activities instrument (Chengdu TME Technology Co., Ltd.), TH-86-340-WA-80 DEG C of ultra cold storage freezer (Germany), 1/0,000 Sartorius electronic analytical balances (Germany); 1/000 electronic analytical balances (Germany); 250Q fixator of rat; The wooden spacious case of uncovered etc.
2 methods
2.1 experiment
2.1.1 grouping and administration are chosen 96 mices and are divided into 8 groups by body weight stratified random, i.e. normal (0.5%CMC-Na20mlkg-1d-1), positive control (Sertraline, 5mgkg-1d-1), HLS and CF1,2,3,4,5(all gives corresponding oral liquid 20mlkg-1d-1) totally 8 groups, 12/group, continuous gastric infusion 7d respectively.
2.1.2 1h after the last administration of tail-suspention test, is suspended on iron stand by mousetail 1-2cm place, with immobilization with adhesive tape, allows mice keep upset hanging posture to adapt to 1min, is determined at the dead time in 5min.
2.1.3 swimming test chooses 96 Kunming mouses, by 2.1.1 step, after last administration 1h, mice is placed in the cuboid white plastic box of water temperature 25 ± 1 DEG C, water capacity 20 × 10 × 13cm3, swimming 6min, is suspended from the water surface dead time after measuring in 5min.
2.2 statistical analysis experimental datas with
represent, SPSS17.0 software kit carries out one factor analysis of variance to data, variance test homogeneous, and p>0.05 uses LSD method, and p<0.05 adopts DunnettT3 method, and p<0.05 is obvious difference.* represent that p<0.05, # represent P<0.05 compared with model group compared with normal group.
3 results
The impact of each Experimental agents on mouse tail suspension and non-swimming time the results are shown in Table 1.
Table 1 is on the impact of mouse tail suspension and non-swimming time
| Group | Outstanding tail time (TST)/s | Dead time (FST)/s |
| Normal group | 138.20±53.46 | 94.00±54.23 |
| Sertraline | 82.60±66.70* | 52.73±34.59* |
| HLS | 86.90±54.30* | 59.91±34.77* |
| CF1 | 117.78±35.22 | 98.00±40.81 |
| CF2 | 117.54±63.14 | 80.82±47.16 |
| CF3 | 107.00±51.15 | 80.18±35.35 |
| CF4 | 81.22±29.73* | 64.00±23.50 |
| CF5 | 121.89±58.28 | 89.45±42.71 |
From table 1, with normal group ratio, HLS and CF4 group significantly can shorten the mouse tail suspension dead time (P<0.05), and all the other organize equal no difference of science of statistics (P>0.05); HLS group significantly can shorten mice non-swimming time (P<0.05), and CF4 group has shortening dead time trend (P=0.083), and all the other are respectively organized all without impact (P>0.05).
Experimental example 2
1 material
The complete male Wistar rat 120 of 1.1 animal SPF level, in 7 ~ 8 week age, weight 200 ± 20g, Da Shuo bio tech ltd, Chengdu provides, the quality certification: SCXK (river) 2008-24, and experiment prospective adaptation is raised 1 week.Feeding environment, room temperature 20 ~ 25 DEG C, humidity 55 ~ 70%, drinking-water of freely ingesting.
1.2 medicines and reagent
Control drug: sertraline hydrochloride sheet (Sertraline) (110801), Dalian pfizer inc, prepared before use becomes the 0.5%CMC-Na suspension of 37.5,62.5%.
Experimental agents 1:HLS, with experimental example 1;
Experimental agents 2:CF4 is that embodiment 1 prepares oral liquid.
Other reagent: autogamy 0.5%CMC-Na liquid; 5-hydroxy tryptamine (5-HT), norepinephrine (NE), dopamine (DA), thyroliberin (ACTH), corticosterone (CORT) and β endorphins (β-EP) Elisa detection kit (201204), by upper Haifeng county, Xiang bio tech ltd provides; Analytical pure PBS, chloral hydrate, paraformaldehyde etc.
1.3 key instrument MK3 microplate reader (U.S. Thermo); AllegraX-22RCentrifuge(U.S. Backman); 81m-25 optical microscope (Olympus company); ZZ-6 mice autonomic activities instrument (Chengdu TME Technology Co., Ltd.), TH-86-340-WA-80 DEG C of ultra cold storage freezer (Germany), 1/0,000 Sartorius electronic analytical balances (Germany); 1/000 electronic analytical balances (Germany); 250Q fixator of rat; The wooden spacious case of uncovered etc.
2 methods
2.1 experiment
2.1.1 grouping and administration
Male Wistar rat 120, do neurological deficit score by Open-field experiment, the rat choosing score 30 ~ 120 points enters experiment, according to the screening experiment result of mice, by Open-field experiment scoring, adopt randomized block design, be divided into 5 groups, often organized more than 16.1. normal group: normally raise; 2. model group: accept lonely the supporting of 21dCUMS+ and prepare depression model; 3. positive drug group: depression model+Sertraline (Sertraline); 4. HLS group: depression model+HLS; 5. CF4 group: depression model+CF4.After modeling 21d, start continuous gastric infusion 21d respectively, and continue modeling.Each group gives corresponding medicine 20mlkg-1d-1 respectively.
2.1.2CUMS+ lonely supporting sets up rat depression model
Except normal group is normally raised (5 ~ 6/cage), other adopts lonely supporting, and gives two kinds of different stimulations every day at random, comprising: 1. cold-wate swimming (4 DEG C, 5min.Depth of water 10cm, moves into rat in 25 ~ 30 DEG C of heated pools after swimming terminates after its limbs are got warm again after a cold spell and takes out); 2. thermostimulation (hot plate method 45 DEG C, 5min); 3. prohibit water (24h); 4. fasting (24h) (can't help water during fasting, non-fasting during taboo water); 5. put upside down round the clock (24h); 6. moist bedding and padding (l0h); 7. fetter 1h; 8. shake (1min).
2.1.3Open-Field mark 100cm × 100cm × 50cm wooden case, bottom is divided into 5 × 5 grids, all paints black in wooden case.The environment of keeping quite, is placed in wooden case central authorities by rat, observe the active situation of rat in 3min, the horizontal score of record rat (at least 3 pawls enter grid pass through lattice number) and vertical score (both feet leave bottom surface) summation.
2.1.4 experimental index detect and method generally all after medicine 1h detect the same day index.
2.1.4.1Open-Field an Open-field experiment score and body weight is measured weekly after experiment and body weight administration.
2.1.4.2 Main Organ Coefficients measures 1h after 21d administration, often organize and get 8 Mus (8:00 ~ 10:30), lumbar injection 10% chloral hydrate (0.3mL/100g) is anaesthetized, femoral artery is got blood and is put to death, immediately ice platform breaks end, get bilateral hippocampus, separately win adrenal gland, thymus, spleen, weigh respectively, calculate organ coefficient.
2.1.4.3 aforementioned the got blood of the assay of Hippocampus and serum 5-HT, NE, DA, leaves standstill the about centrifugal 20min of about 1h, 3000r/min, gets supernatant.Aforementioned got Hippocampus, adds a certain amount of PBS, homogenate, ditto centrifugal, gets supernatant, and-80 DEG C of freezen protective are to be measured.The assay of 5-HT, DA, NE is undertaken by test kit specification limit.
2.1.4.4 the mensuration of assay ACTH, CORT, β-EP of serum ACTH, CORT, β-EP is undertaken by test kit specification limit.
2.1.4.5 PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM respectively organizes residue 8 rats, with 2.5 anesthesia, lie on the back fixing, be about 100ml through left ventricle saline injection, shred liver, reinject 4% paraformaldehyde perfusion fixation, get brain, adrenal gland, weigh respectively.Fixing in specimen 4% paraformaldehyde liquid, paraffin embedding, be interrupted section, every 10 sections get 1, and each Hippocampus and adrenal gland respectively get 5, and HE dyes.Adopt TSView7 image analysis system (TUCSENIMAGINGTECHNOLOGY, Inc.), under 400 times of light microscopics, hippocampal slices CA3 district counts neuron population in 5 lattices and normal neurons number, calculates the normal neurons number ratio of Hippocampus; Under 40 times of light microscopics, measure adrenocortical maximum gauge.
2.2 statistical analysis experimental datas with
represent, SPSS17.0 software kit carries out one factor analysis of variance to data, variance test homogeneous, and p>0.05 uses LSD method, and p<0.05 adopts DunnettT3 method, and p<0.05 is obvious difference.* represent that p<0.05, # represent P<0.05 compared with model group compared with normal group.
3 results
3.1 on open-field scoring and the impact of body weight change
The results are shown in Table 2.Result shows, compares with normal group, and the times of exercise of model group rats reduces (P<0.05); With model group ratio, administration is after 1 week, HLS and CF4 does not obviously increase times of exercise (P>0.05), after 2 weeks, there is increase trend, HLS (P=0.064), CF4 (P=0.087), after 3 weeks, HLS is significantly increased times of exercise effect (P<0.05), and CF4 has increase trend (P=0.093).Model group rats body weight compared with normal group significantly reduces (P<0.05); Compared with model group, administration 1,2,3 weeks, HLS and CF4 is all shown in that body weight significantly increases (P<0.05).Illustrate that HLS has obvious treatment to depression rat behavioristics, CF4 has certain improvement result.
Table 2 is on open-field scoring and the impact of body weight change
3.2 impacts on monoamine neurotransmitter in rat blood serum and brain
The results are shown in Table 3.Result shows, with Normal group ratio, model group rats Hippocampus monoamine neurotransmitter content reduces (p<0.05), and in blood, content raises (p<0.05).Compare with model group, the content of HLS group Hippocampus 5-HT and DA raises (p<0.05), and the content of CF4 group Hippocampus DA raises (p<0.05).In HLS and CF4 group blood, NE, DA, 5-HT all reduce (p<0.05).
Table 3 is on the impact of monoamine neurotransmitter in rat blood serum and brain
3.3 impacts on rat blood serum ACTH, CORT, β-EP
The results are shown in Table 4.Result shows all remarkable than the normal group rising (P<0.05) of the content of model group serum ACTH, CORT, β-EP.Through HLS, CF4 treatment, serum ACTH, CORT, β-EP all has obvious reduction (P<0.05).
Table 4 is on the impact of rat blood serum ACTH, CORT, β-EP
| Group | ACTH/ng.L -1 | CORT/ng.L -1 | β-EP/ng.L -1 |
| Normal group | 42.98±6.72 | 14.81±4.27 | 200.94±59.10 |
| Model group | 64.29±15.53 * | 24.54±6.95 * | 282.93±72.49 * |
| Model+Sertraline group | 47.04±11.34 # | 19.77±3.82 # | 201.78±34.42 # |
| Model+HLS group | 43.48±11.11 # | 17.83±5.51 # | 184.23±37.73 # |
| Model+CF4 group | 49.19±13.99 # | 18.36±7.21 # | 195.83±67.93 # |
3.4 impacts on Rats Organs and Tissues coefficient
The results are shown in Table 5.Result shows, model group obviously increases (p<0.05), thymus coefficient than adrenal gland's coefficient of normal rats, Spleen coefficient reduces (p<0.05).Through treatment, HLS has certain reduction trend (P=0.093) to adrenal gland's coefficient, obvious increase effect (p<0.05) is had to thymus coefficient and Spleen coefficient, CF4 without obvious effect (p>0.05), has increase tendency to thymus coefficient (P=0.053) and Spleen coefficient (P=0.058) to adrenal gland's coefficient.
Table 5 is on the impact of Rats Organs and Tissues coefficient
| Group | Adrenal gland's coefficient | Thymus coefficient | Spleen coefficient |
| Normal group | 0.0160±0.0021 | 0.1574±0.0271 | 0.4256±0.1381 |
| Model group | 0.0274±0.0182* | 0.1003±0.0466* | 0.2096±0.0464* |
| Model+Sertraline group | 0.0239±0.0071 | 0.1281±0.0485 | 0.2466±0.0520 |
| Model+HLS group | 0.0212±0.0055 | 0.1424±0.0458# | 0.2914±0.1539# |
| Model+CF4 group | 0.0232±0.0070 | 0.1381±0.0743 | 0.2881±0.1337 |
3.5 impacts on rat hippocampus, adrenal gland's morphological changes of various tissue components
3.5.1HLS with the impact of CF4 on Hippocampus CA 3 Region neuron morphology and number
Normal rats Hippocampus CA 3 Region neuronal cell marshalling, intensive, kernel is high-visible.Compared with normal group, the Hippocampus CA 3 Region neuronal cell arrangement of model group rats is sparse, and intercellular substance increases, and kernel disappears, and cytoplasmic condensati, is shown in Fig. 1.The neuron number of model group and the ratio compared with normal group shared by normal neurons significantly reduce (P<0.05), compared with model group, the ratio of HLS and CF4 group Hippocampus CA 3 Region neuron number and normal neurons obviously increases (P<0.05), in table 6.
3.5.2HLS with the impact of CF4 on Rat Adrenal Cortex thickness and adrenal gland's pathological change
Normal rat adrenal gland clear in structure, ecto-entad is divided into adrenal cortex and medullary substance part, and wherein cortex is divided into glomerular zone again, zona fasciculata and reticular zone; CUMS rat cortex is plump.The adrenal cortex thickness of model group compared with normal group rat obviously increases (P<0.05), HLS and CF4 group obviously can reduce the adrenal cortex thickness (P<0.05) of model mouse, in table 6.
Table 6 is on the impact of rat hippocampus, adrenal gland's morphological changes of various tissue components
| Group | Neuron number | The ratio (%) of normal neurons | Adrenal cortex thickness (mm) |
| Normal group | 47.80±4.38 | 90.31±11.22 | 793.33±92.45 |
| Model group | 33.00±5.52 | 34.51±19.04 * | 976.00±105.97 * |
| Model+Sertraline group | 46.25±7.93 | 77.76±15.48 # | 810.12±96.09 # |
| Model+HLS group | 44.80±8.53 | 73.75±11.77 # | 821.11±112.41 # |
| Model+CF4 group | 43.67±7.84 | 67.26±14.77 # | 841.43±106.54 # |
Detailed description of the invention
Embodiment 1 oral liquid
Herba Epimedii 300g, Radix Astragali 440g, Radix Polygoni Multiflori Preparata 1000g;
Take epimedium herb 300g, add 10 times of water gagings, boil and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add ethanol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate regulates pH value to 8.0 with 10% sodium hydroxide solution, leaves standstill 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Take Radix Astragali 440g, add 10 times amount water extraction three times, each 1.5 hours, merge extractive liquid, filter, concentrated for subsequent use; Take 1000g Radix Polygoni Multiflori Preparata, add decocting in water and carry three times, merging filtrate, filter, heating is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 70%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 80%, then leaves standstill 24 hours, filters, filtrate regulates PH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Get extraction ofHerba Epimedii, Radix Astragali extractive solution, Radix Polygoni Multiflori Preparata extracting solution stir evenly, cold preservation places 12 hours, filters, adds water to 1000ml, filters, and filtrate is by 10% sodium hydroxide adjust ph to 7.0, and heated and boiled sterilizing, to obtain final product.
Embodiment 2 tablet
Herba Epimedii 25g, Radix Astragali 22g, Radix Polygoni Multiflori Preparata 50g;
Get the crude drug of recipe quantity, add 12 times amount 70% alcohol reflux 2 times, each 1.5 hours; Merge extractive liquid, filters, and reclaims ethanol and concentrates; Concentrated solution drying under reduced pressure, is ground into fine powder, adds customary adjuvant, mixing; Dry-pressing is granulated, and tabletting, to obtain final product.
Embodiment 3 capsule
Herba Epimedii 15g, Radix Astragali 36g, Radix Polygoni Multiflori Preparata 50g;
Get the crude drug of recipe quantity, add 10 times amount 80% ethanol ultrasonic extraction 3 times, for the first time 40min, the 2nd, 3 each 20min, merge extractive liquid, filter, concentrating under reduced pressure; Concentrated solution drying under reduced pressure, is ground into fine powder, adds customary adjuvant, mixing; Dry-pressing is granulated, and incapsulates, to obtain final product.
Embodiment 4 granule
Herba Epimedii 8g, Radix Astragali 50g, Radix Polygoni Multiflori Preparata 20g;
Get the crude drug of recipe quantity, decoct with water 2 times, 2 hours first times, second time 1.5 hours, collecting decoction, filter, filtrate concentrates, and it is 80% that concentrated solution adds ethanol to alcohol content, leaves standstill, and filters, filtrate recycling ethanol is extremely without alcohol taste, and concentrate drying, be ground into fine powder, add adjuvant, mixing; Dry-pressing is granulated, and to obtain final product.
Embodiment 5 oral liquid
Herba Epimedii 35g, Radix Astragali 10g, Radix Polygoni Multiflori Preparata 75g;
Get epimedium herb, add 8 times amount 70% alcohol reflux 2 times, each 1.5 hours; Merge extractive liquid, filters, and filtrate regulates pH value to 9.0 with 10% sodium hydroxide solution, leaves standstill 24 hours, filters, and filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Get Radix Astragali medicine merge extractive liquid, filter, concentrated for subsequent use; Get Radix Polygoni Multiflori Preparata medical material, add water backflow three times, merging filtrate, filter, heating is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 60%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 90%, then leaves standstill 24 hours, filters, filtrate regulates pH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, for subsequent use; Get extraction ofHerba Epimedii, Radix Astragali extractive solution, Radix Polygoni Multiflori Preparata extracting solution stir evenly, cold preservation places 12 hours, filters, and filtrate is by 10% sodium hydroxide adjust ph to 7.0, and heated and boiled sterilizing, to obtain final product.
Embodiment 6 drop pill
Herba Epimedii 9g, Radix Astragali 45g, Radix Polygoni Multiflori Preparata 25g;
Get recipe quantity Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata medical material, respectively with 60% ethanol ultrasonic extraction, extracting solution concentrates to obtain Herba Epimedii extract, Radix Astragali extract and Radix Polygoni Multiflori Preparata extract, by three's mix homogeneously, adds customary adjuvant and makes drop pill according to the routine techniques of this area preparation.
Embodiment 7 tablet
Herba Epimedii 30g, Radix Astragali 12g, Radix Polygoni Multiflori Preparata 70g;
Get recipe quantity Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata medical material, respectively with 12 times of water gaging reflux, extract, extracting solution concentrates to obtain Herba Epimedii extract, Radix Astragali extract and Radix Polygoni Multiflori Preparata extract, by three's mix homogeneously, adds customary adjuvant, and dry-pressing is granulated, and tabletting, to obtain final product.
Embodiment 8
Herba Epimedii extract 15g, Radix Astragali extract 22g, Radix Polygoni Multiflori Preparata extract 50g;
The extract that described Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract are respectively Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata prepare through 60% alcohol reflux.To pulverize after said extracted thing drying under reduced pressure as fine powder, mixing, adds customary adjuvant and makes granule.
Embodiment 9
Herba Epimedii extract 15g, Radix Astragali extract 36g, Radix Polygoni Multiflori Preparata extract 50g;
The extract that described Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract are respectively Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata prepare through soak by water.To pulverize after said extracted thing drying under reduced pressure as fine powder, mixing, adds customary adjuvant and makes tablet.
Embodiment 10
Herba Epimedii extract 12g, Radix Astragali extract 30g, Radix Polygoni Multiflori Preparata extract 60g;
The extract that described Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract are respectively Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata prepare through aqueous extraction-alcohol precipitation technology, its alcohol precipitation concentration is the extract that 70% alcoholic solution seepage pressure effects obtains; Be ground into fine powder by after said extracted thing drying under reduced pressure, add customary adjuvant, mixing; Dry-pressing is granulated, and tabletting, to obtain final product.
Embodiment 11
Herba Epimedii extract 35g, Radix Astragali extract 10g, Radix Polygoni Multiflori Preparata extract 70g;
Described Herba Epimedii extract is adopted and is prepared with the following method: get epimedium herb, add 10 times of water gagings, boil and carry twice, each 1.5 hours, filter, merging filtrate, leave standstill, get supernatant concentration to appropriate, add ethanol after letting cool and reach 65% to alcohol content, leave standstill 24 hours, filter, filtrate adds ethanol makes alcohol content reach 80%, leave standstill, 24 hours, filter, filtrate is by 10% sodium hydroxide solution adjust ph to 8.0, leave standstill 24 hours, filter, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaim ethanol, obtain Herba Epimedii extract;
Described Radix Astragali extract is adopted and is prepared with the following method: get Milkvetch Root, add 10 times amount water extraction three times, each 1.5 hours, merge extractive liquid, filters, concentrated, obtains Radix Astragali extract;
Described Radix Polygoni Multiflori Preparata extract is adopted and is prepared with the following method: get Radix Polygoni Multiflori Preparata medical material, add decocting in water and carry three times, merging filtrate, filter, heating is concentrated into syrupy shape, lets cool, adding ethanol makes alcohol content reach 70%, leaves standstill 12 hours, filters, filtrate adds ethanol again makes alcohol content reach 80%, then leaves standstill 24 hours, filters, filtrate regulates pH to 8.0 with the NaOH of 10%, places 24 hours, filters, filtrate regulates pH to 7.0 with the hydrochloric acid of 10%, reclaims ethanol, obtains Radix Polygoni Multiflori Preparata extract.
The above-mentioned three kinds of extracts prepared are mixed, is ground into fine powder after drying under reduced pressure, adds customary adjuvant, mixing; Dry-pressing is granulated, and tabletting, to obtain final product.
Claims (14)
1. a Chinese medicine composition for Cure of depression, is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 8-35 weight portion, Radix Polygoni Multiflori Preparata 20-75 weight portion, Radix Astragali 10-50 weight portion.
2. Chinese medicine composition as claimed in claim 1, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 9-30 weight portion, Radix Polygoni Multiflori Preparata 25-70 weight portion, Radix Astragali 12-45 weight portion.
3. Chinese medicine composition as claimed in claim 1, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 10-25 weight portion, Radix Polygoni Multiflori Preparata 30-65 weight portion, Radix Astragali 15-35 weight portion.
4. Chinese medicine composition as claimed in claim 1, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 10-20 weight portion, Radix Polygoni Multiflori Preparata 35-65 weight portion, Radix Astragali 15-27 weight portion.
5. Chinese medicine composition as claimed in claim 1, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii 15 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 22 weight portion;
Or, Herba Epimedii 25 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 22 weight portion;
Or, Herba Epimedii 15 weight portion, Radix Polygoni Multiflori Preparata 50 weight portion, the Radix Astragali 36 weight portion.
6. the preparation method of Chinese medicine composition as described in as arbitrary in claim 1-5, it is characterized in that, the method comprises:
Get crude drug in proportion, with water or the organic solvent extraction that dissolves each other with water; Or, get crude drug in proportion, adopt decoction and alcohol sedimentation technique preparation.
7. the preparation method of Chinese medicine composition as described in as arbitrary in claim 1-5, it is characterized in that, the method comprises:
Get Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata crude drug in proportion;
Respectively with water extraction, or with the organic solvent extraction dissolved each other with water, or carry out water extract-alcohol precipitation, prepare Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract; And
Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract are merged.
8. preparation method as claimed in claim 7, it is characterized in that, the method comprises the steps:
A, get epimedium herb, water boiling and extraction, extracting solution adds ethanol precipitate with ethanol, prepares Herba Epimedii extract;
B, get Milkvetch Root, water boiling and extraction, extracting solution is concentrated to obtain Radix Astragali extract;
C, get Radix Polygoni Multiflori Preparata medical material, water boiling and extraction, extracting solution adds ethanol precipitate with ethanol, prepares Radix Polygoni Multiflori extract; And
D, get Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract, mixing.
9. preparation method as claimed in claim 8, is characterized in that:
A, get epimedium herb, water boiling and extraction, it is 60%-80% that extracting solution adds ethanol to alcohol content, filters, and filtrate regulates pH to be alkalescence, filters, and filtrate regulates pH to neutral, reclaims ethanol, obtains Herba Epimedii extract;
B, get Milkvetch Root, water boiling and extraction, extracting solution is concentrated to obtain Radix Astragali extract;
C, get Radix Polygoni Multiflori Preparata medical material, water boiling and extraction, it is 70%-80% that extracting solution adds ethanol to alcohol content, filters, and filtrate regulates pH to be alkalescence, filters, and filtrate regulates pH to neutral, reclaims ethanol, obtains Radix Polygoni Multiflori Preparata extract; And
D, get Herba Epimedii extract, Radix Astragali extract, Radix Polygoni Multiflori Preparata extract, mixing.
10. a Chinese medicine composition for Cure of depression, is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii extract 8-35 weight portion, Radix Polygoni Multiflori Preparata extract 20-75 weight portion, Radix Astragali extract 10-50 weight portion.
11. Chinese medicine compositions as claimed in claim 10, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii extract 9-30 weight portion, Radix Polygoni Multiflori Preparata extract 25-70 weight portion, Radix Astragali extract 12-45 weight portion.
12. Chinese medicine compositions as claimed in claim 10, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii extract 10-25 weight portion, Radix Polygoni Multiflori Preparata extract 30-65 weight portion, Radix Astragali extract 15-35 weight portion.
13. Chinese medicine compositions as claimed in claim 10, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii extract 10-20 weight portion, Radix Polygoni Multiflori Preparata extract 35-65 weight portion, Radix Astragali extract 15-27 weight portion.
14. Chinese medicine compositions as claimed in claim 10, it is characterized in that, the crude drug of this Chinese medicine composition consists of:
Herba Epimedii extract 15 weight, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 22 parts;
Or, Herba Epimedii extract 25 weight portion, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 22 weight portion;
Or, Herba Epimedii extract 15 weight portion, Radix Polygoni Multiflori Preparata extract 50 weight portion, Radix Astragali extract 36 weight portion.
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| CN101559168A (en) * | 2008-04-17 | 2009-10-21 | 成都地奥集团天府药业股份有限公司 | Pharmaceutical composition for invigorating Qi, tonifying blood and nourishing liver and kidney as well as preparation method and application thereof |
| CN102397462A (en) * | 2010-09-10 | 2012-04-04 | 成都华神集团股份有限公司制药厂 | Oral liquid used for resisting aging, preparation method thereof, and quality controlling method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559168A (en) * | 2008-04-17 | 2009-10-21 | 成都地奥集团天府药业股份有限公司 | Pharmaceutical composition for invigorating Qi, tonifying blood and nourishing liver and kidney as well as preparation method and application thereof |
| CN101496865A (en) * | 2009-03-19 | 2009-08-05 | 杨颖� | Chinese medicament preparation for treating depression and anxiety as well as preparation method thereof |
| CN102397462A (en) * | 2010-09-10 | 2012-04-04 | 成都华神集团股份有限公司制药厂 | Oral liquid used for resisting aging, preparation method thereof, and quality controlling method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 活力苏口服液对自然衰老小鼠肝、脑、肾组织p16基因表达的影响;林欣等;<<现代中西医结合杂志>>;20130131;第22卷(第2期);142-143,226 * |
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| CN104069188A (en) | 2014-10-01 |
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