CN104067724A - Method for safely breaking pokeberry root seed episperm - Google Patents
Method for safely breaking pokeberry root seed episperm Download PDFInfo
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- CN104067724A CN104067724A CN201410300296.9A CN201410300296A CN104067724A CN 104067724 A CN104067724 A CN 104067724A CN 201410300296 A CN201410300296 A CN 201410300296A CN 104067724 A CN104067724 A CN 104067724A
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- sulfuric acid
- concentrated sulfuric
- cup
- phytolacca acinosa
- seed
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Abstract
The invention relates to a method for safely breaking pokeberry root seed episperm. The method comprises the following steps: arranging pokeberry root seeds in an inner cup of which the bottom and side walls are provided with small holes, arranging the inner cup in an outer cup which has the same size as the inner cup and is filled with concentrated sulfuric acid, soaking for a certain time, and taking out the inner cup; and arranging the inner cup in a plastic cup which has the same size as the inner cup and is filled with distilled water, and cleaning residual concentrated sulfuric acid on the surfaces of the pokeberry root seeds to prevent the pokeberry root seeds from being damaged by heat dissipated after the operation of dissolving lots of concentrated sulfuric acid in water. The method is simple in steps and easy to operate, the germination rate of the pokeberry root seeds can be effectively improved, the inner cup is only moved by an operator in the whole process, and damage of concentrated sulfuric acid on the operator can be reduced. Meanwhile, concentrated sulfuric acid and diluted acid liquor are collected in the plastic cup and do not enter an external environment at all, and thus the environmental pollution is avoided.
Description
Technical field
The present invention relates to Plant Tissue Breeding and heavy metal hyperaccumulative plant and cultivate field, the safety that relates in particular to a kind of Phytolacca acinosa seed exosper is abolished method.
Background technology
Heavy metal pollution is due to its high poison, lasting and be difficult for being degraded into the soil pollutant of global concern.The reparation that heavy metal in soil pollutes is thought one of the most difficult technology by many scholars.The extractive technique of heavy metal hyperaccumulative plant is considered to the most thoroughly, economic, eco-friendly feature is by science researcher, Environmental Engineer's common concern.Phytolacca acinosa is because its biomass is large, growth is fast and can become heavy metal pollution of soil desirable plants by excess accumulation heavy metal in soil.Because the sprouting of trade route seed is more difficult, Phytolacca acinosa is mainly that plantation and research in laboratory, trade route are restricted by plucking the method for the serious mining area self-sow of heavy metal pollution in the research of soil pollution by heavy metal reparation at present.
Phytolacca acinosa is by seminal propagation, but under the condition of culture of the structure of its exosper hard and general temperature and humidity very, is difficult to destroy, and very low of its germination rate, has limited widely it and in heavy metal pollution, repaired the application in field.Usual way is by methods such as freezing, illumination, potassium nitrate solution, artificial outside destroy, but its germination rate is also very low.Compare with said method, it is a kind of method of very effective destruction Phytolacca acinosa exosper that the concentrated sulfuric acid soaks.
But Phytolacca acinosa seed presents uniform distribution in the concentrated sulfuric acid, be difficult to its and the concentrated sulfuric acid to separate, adopt topple over or the method for the suction pipe absorption concentrated sulfuric acid not only time-consuming but also easily operating personnel are damaged.Add the concentrated sulfuric acid and there is very strong corrosivity, the length of soak time affects percentage of seedgermination, and diluting concentrated sulfuric acid process is strong exothermic process, if misoperation, not in time that the concentrated sulfuric acid is separated with seed, the heat distributing can destroy the inclusion of Phytolacca acinosa seed, soak time can not be destroyed its hard exosper not completely, reduce germination rate, in operating process, the flushing of the outside residual concentrated sulfuric acid of Phytolacca acinosa seed also pollutes the environment in addition, the concentrated sulfuric acid has again strong corrosivity, for experimental implementation has fast and accurately increased difficulty.Therefore, the germination problem of the solution Phytolacca acinosa seed of efficient, safety, environmental protection, becomes the key issue of Phytolacca acinosa in soil pollution reparation application.
Summary of the invention
In order to overcome the above problems; the invention provides and a kind ofly both can realize quickly and accurately the separated of Phytolacca acinosa seed and the concentrated sulfuric acid; can improve the germination rate of Phytolacca acinosa again; can protect operator safety simultaneously; also do not pollute the environment completely, be also the dirt cheap safety of the Phytolacca acinosa seed exosper that is easy to get of material is abolished method.
The present invention is achieved by the following technical solutions:
The safety of above-mentioned Phytolacca acinosa seed exosper is abolished method, comprises the following steps:
(1) immersion of Phytolacca acinosa seed
(1.1) get clean ripe Phytolacca acinosa seed, the Phytolacca acinosa seed of choosing is positioned in a bottom and the foraminate interior cup of sidewall tool; Select again first outer cup with plastic inner cup formed objects, in the first outer cup, contain a certain amount of concentrated sulfuric acid solution; (1.2) the interior cup that Phytolacca acinosa seed is housed in above-mentioned steps S011 is placed in the first outer cup that concentrated sulfuric acid solution is housed, after 6 minutes, interior cup is taken out fast, make Phytolacca acinosa seed separated fast with the concentrated sulfuric acid;
(2) removal of the residual concentrated sulfuric acid
(2.1) select one with above-mentioned steps S011 in onesize the second outer cup of the first outer cup, and in the second outer cup, contain a certain amount of distilled water or running water; (2.2) the interior cup soaking in above-mentioned steps S012 is later placed in to the second outer cup that above-mentioned steps S021 contains distilled water, and shaking gently cleaned the residual concentrated sulfuric acid on the outside and interior wall of cup of Phytolacca acinosa seed, repeat 5-6 time, to remove the residual concentrated sulfuric acid of Phytolacca acinosa the surface of the seed; (2.3) the Phytolacca acinosa seed after above-mentioned steps S022 cleaning is placed on filter paper and is drained away the water, think in medium and cultivate and prepare.
The safety of described Phytolacca acinosa seed exosper is abolished method, wherein: the described concentrated sulfuric acid solution in described step (1.1) is the concentrated sulfuric acid solution that concentration is greater than 70%.
The safety of described Phytolacca acinosa seed exosper is abolished method, wherein: the diameter of the aperture of described interior cup is less than the diameter of Phytolacca acinosa seed.
The safety of described Phytolacca acinosa seed exosper is abolished method, wherein: what described interior cup and described first, second outer cup all adopted is disposable plastics water glass.
Beneficial effect:
It is simple that the safety of Phytolacca acinosa seed exosper of the present invention is abolished method step, processing ease, can effectively improve the germination rate of Phytolacca acinosa, wherein, Phytolacca acinosa seed is placed in to a bottom and the foraminate interior cup of sidewall tool, then interior cup is placed in to the onesize outer cup that fills the concentrated sulfuric acid, the concentrated sulfuric acid can enter by aperture like this, after the immersion regular hour, interior cup is picked up, the concentrated sulfuric acid will flow out interior cup by aperture like this, it is punctual to realize, separated fast, then interior cup is placed in to the onesize outer cup that fills distilled water, wash the residual concentrated sulfuric acid of Phytolacca acinosa the surface of the seed, avoided the injury of the water-soluble heat giving out of a large amount of concentrated sulfuric acids to Phytolacca acinosa seed, meanwhile, in whole process operating personnel just mobile in glass, reduced the harm that the concentrated sulfuric acid causes operating personnel, and the acid solution after the concentrated sulfuric acid and dilution is all collected in outer cup, do not have in an entered environment.The principle that whole method utilization " two a set of cups " is filtered is separated fast with Phytolacca acinosa seed by the concentrated sulfuric acid, and has ensured that operating personnel's safety has reclaimed whole sulfuric acid solutions simultaneously, does not pollute environment, and meanwhile, material is also dirt cheap and is easy to get, and cost is lower.
Embodiment
The safety of Phytolacca acinosa seed exosper of the present invention is abolished method, and it comprises the following steps:
The immersion of S010, Phytolacca acinosa seed
S011, get clean ripe Phytolacca acinosa seed, the Phytolacca acinosa seed of choosing is positioned in a bottom and the foraminate interior cup of sidewall tool; Select again first outer cup with plastic inner cup formed objects, in the first outer cup, contain a certain amount of concentrated sulfuric acid solution (concentrated sulfuric acid that concentration is greater than 70%);
S012, the interior cup that Phytolacca acinosa seed is housed in above-mentioned steps S011 is placed in the first outer cup that concentrated sulfuric acid solution is housed, after 6 minutes, interior cup is taken out fast, make Phytolacca acinosa seed separated fast with the concentrated sulfuric acid;
The removal of S020, the residual concentrated sulfuric acid
S021, select one with above-mentioned steps S011 in onesize the second outer cup of the first outer cup, and in the second outer cup, contain a certain amount of running water or distilled water;
S022, the interior cup soaking in above-mentioned steps S012 is later placed in to the second outer cup that above-mentioned steps S021 contains distilled water, and shaking gently cleaned the residual concentrated sulfuric acid on the outside and interior wall of cup of Phytolacca acinosa seed, repeat 5-6 time, to remove the residual concentrated sulfuric acid of Phytolacca acinosa the surface of the seed;
S023, the Phytolacca acinosa seed after above-mentioned steps S022 is cleaned are placed on filter paper drain away the water (rate of abolishing of this method Phytolacca acinosa seed can reach 69%-98%), think and in medium, cultivate prepare (germination rate can reach 60%-70%).
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1
The immersion of S110, Phytolacca acinosa seed
S111, get 100 clean ripe Phytolacca acinosa seeds, be placed in a bottom and the foraminate plastic inner cup of sidewall tool; Select again a first plastics outer cup with plastic inner cup formed objects (volume is 200ml), in the first plastics outer cup, contain the concentrated sulfuric acid solution of 20mL;
S112, the plastic inner cup that Phytolacca acinosa seed is housed in above-mentioned steps S111 is placed in the first plastics outer cup that concentrated sulfuric acid solution is housed, after 6 minutes, plastic inner cup is taken out fast, make Phytolacca acinosa seed separated fast with the concentrated sulfuric acid;
The removal of S020, the residual concentrated sulfuric acid
S021, select one with above-mentioned steps S011 in onesize the second outer cup of the first outer cup, and in the second outer cup, contain the distilled water of 45mL;
S022, the interior cup soaking in above-mentioned steps S012 is later placed in to the second outer cup that above-mentioned steps S021 contains distilled water, and shaking gently cleaned the residual concentrated sulfuric acid on the outside and interior wall of cup of Phytolacca acinosa seed, repeat 5-6 time, to remove the residual concentrated sulfuric acid of Phytolacca acinosa the surface of the seed;
S023, the Phytolacca acinosa seed after above-mentioned steps S022 is cleaned are placed on filter paper drain away the water (rate of abolishing of Phytolacca acinosa seed can reach 98%), think in medium and cultivate and prepare.
Phytolacca acinosa seed after the present embodiment 1 is abolished is transferred to above the clean perlite of distilled water, cover one deck vermiculite above, after about one week, start to sprout and add 1/4 Hoagland culture fluid, add gradually 1/2 later, full culture fluid was cultivated after one month, and Phytolacca acinosa height of seedling can reach 20cm left and right.The method of the present embodiment 1 is processed germination rate can reach 60%～70%, than physical method, processes and improves 20%～40%, and germination rate and survival rate are all higher; Meanwhile, it is simple, convenient that whole Phytolacca acinosa seed exosper is abolished method, ensured operating personnel's safety, also reclaimed whole sulfuric acid solutions, do not pollute environment.
Wherein, except the above embodiments 1, adopt the method for abolishing of the present invention May of 2007 and June, time experiment to be carried out to a kind skin with Phytolacca acinosa seed in two batches and abolish experiment, 50 seeds of each sampling observation, check the rate of abolishing of its kind of skin, the rate of abolishing can reach respectively 96% (48/50) and 98% (49/50), and tests whole process at every turn and be about 30 minutes, and conventional efficient is much higher than other the method for abolishing.
It is simple, easy to operate that the safety of Phytolacca acinosa seed exosper of the present invention is abolished method step, wherein, the principle of utilizing " two a set of cups " to filter is separated fast with Phytolacca acinosa seed by the concentrated sulfuric acid, has ensured operating personnel's safety, also reclaim whole sulfuric acid solutions, do not polluted environment.
Claims (4)
1. the safety of Phytolacca acinosa seed exosper is abolished a method, it is characterized in that, comprises the following steps:
(1) immersion of Phytolacca acinosa seed
(1.1) get clean ripe Phytolacca acinosa seed, the Phytolacca acinosa seed of choosing is positioned in a bottom and the foraminate interior cup of sidewall tool; Select again first outer cup with plastic inner cup formed objects, in the first outer cup, contain a certain amount of concentrated sulfuric acid solution;
(1.2) the interior cup that Phytolacca acinosa seed is housed in above-mentioned steps S011 is placed in the first outer cup that concentrated sulfuric acid solution is housed, after 6 minutes, interior cup is taken out fast, make Phytolacca acinosa seed separated fast with the concentrated sulfuric acid;
(2) removal of the residual concentrated sulfuric acid
(2.1) select one with above-mentioned steps S011 in onesize the second outer cup of the first outer cup, and in the second outer cup, contain a certain amount of distilled water or running water;
(2.2) the interior cup soaking in above-mentioned steps S012 is later placed in to the second outer cup that above-mentioned steps S021 contains distilled water, and shaking gently cleaned the residual concentrated sulfuric acid on the outside and interior wall of cup of Phytolacca acinosa seed, repeat 5-6 time, to remove the residual concentrated sulfuric acid of Phytolacca acinosa the surface of the seed;
(2.3) the Phytolacca acinosa seed after above-mentioned steps S022 cleaning is placed on filter paper and is drained away the water, think in medium and cultivate and prepare.
2. the safety of Phytolacca acinosa seed exosper as claimed in claim 1 is abolished method, it is characterized in that: the described concentrated sulfuric acid solution in described step (1.1) is the concentrated sulfuric acid solution that concentration is greater than 70%.
3. the safety of Phytolacca acinosa seed exosper as claimed in claim 1 is abolished method, it is characterized in that: the diameter of the aperture of described interior cup is less than the diameter of Phytolacca acinosa seed.
4. the safety of Phytolacca acinosa seed exosper as claimed in claim 1 is abolished method, it is characterized in that: what described interior cup and described first, second outer cup all adopted is disposable plastics water glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410300296.9A CN104067724A (en) | 2014-06-26 | 2014-06-26 | Method for safely breaking pokeberry root seed episperm |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410300296.9A CN104067724A (en) | 2014-06-26 | 2014-06-26 | Method for safely breaking pokeberry root seed episperm |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002058538A2 (en) * | 2001-01-26 | 2002-08-01 | Regeneron Pharmaceuticals, Inc. | Methods of imaging and targeting vasculature |
| WO2003022029A1 (en) * | 2001-09-12 | 2003-03-20 | Virens Di Baggio Nicola & Andrea S.N.C. | Item for the cultivation of an agricultural plantation and method for making said item |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN202979622U (en) * | 2012-12-18 | 2013-06-12 | 山东省农作物种质资源中心 | Drug seed soaking treatment instrument |
-
2014
- 2014-06-26 CN CN201410300296.9A patent/CN104067724A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002058538A2 (en) * | 2001-01-26 | 2002-08-01 | Regeneron Pharmaceuticals, Inc. | Methods of imaging and targeting vasculature |
| WO2003022029A1 (en) * | 2001-09-12 | 2003-03-20 | Virens Di Baggio Nicola & Andrea S.N.C. | Item for the cultivation of an agricultural plantation and method for making said item |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN202979622U (en) * | 2012-12-18 | 2013-06-12 | 山东省农作物种质资源中心 | Drug seed soaking treatment instrument |
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Application publication date: 20141001 |