CN1040657C - Method of extracting superoxide dismutase (SOD) from corn - Google Patents
Method of extracting superoxide dismutase (SOD) from corn Download PDFInfo
- Publication number
- CN1040657C CN1040657C CN93115908A CN93115908A CN1040657C CN 1040657 C CN1040657 C CN 1040657C CN 93115908 A CN93115908 A CN 93115908A CN 93115908 A CN93115908 A CN 93115908A CN 1040657 C CN1040657 C CN 1040657C
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- sod
- corn
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a method for extracting SOD from corn, which has the technical scheme of disinfecting the raw material and washing the raw material with water; subsequently, immersing the raw material in water with the temperature of 20 to 30 DEG C for 20 to 60 hours, adding phosphate buffer and PVP, homogenizing, filtering, and ultra-filtering for concentration; then, adding antioxidants and stabilizers; finally, disinfecting and packaging the product. The present invention has the advantage of SOD extraction from the corn, simple manufacturing process, short production cycle, low cost, wide raw material sources and sanitary and safe use; the SOD can be applied to medicine, food, cosmetics, etc.
Description
The invention belongs to the method for industrialization extraction biochemicals from plant, is a kind of processing method of extracting superoxide-dismutase from corn.
Superoxide-dismutase (SOD) extensively is present in the organism, and the disproportionation reaction of its Catalytic Oxygen free radical can be removed the superoxide radical that organism is had strong toxic action, and organism is shielded.People have studied SOD to anti-inflammatory, anti-ageing, Antiradiation injury and antineoplastic action in recent years, and SOD have been applied to many fields such as medicine, makeup and food.SOD is a kind of zymin that the exploitation future is arranged very much.
At present, commercially available SOD is preparation from animal blood or tissue, and the main technique content of preparation is: fragmentation, sulphur ammonium precipitation, ultrafiltration, chromatography, concentrated, freeze-drying.Through international online retrieval world patent, United States Patent (USP), Chinese patent, biological abstracts, american agriculture digest, CAB Abstracts, U.S. chemical abstract and 4 kinds of domestic professional digests, the discovery that does not all have does not have to find to extract the document of the suitability for industrialized production of SOD than the more effective document introduction of simple economy of aforesaid method from corn.
The purpose of this invention is to provide a kind of simplified method that from corn, extracts SOD, this method work simplification, economize desulfuration ammonium precipitation, chromatography, concentrate, step such as lyophilize, shortened the production cycle, reduce production costs, be fit to batch production production, and raw material sources are wide,, safety more much cleaner than animal blood.The SOD that uses the present invention's preparation is applicable to external application medicine, food, makeup etc.
Main contents of the present invention are as follows:
1, maize raw material carries out surface sterilization with clorox;
2, sterilization back flushing with clean water;
3, cleaning the back soaked 20-60 hour in 20-30 ℃ of water;
4, soak back adding phosphoric acid buffer and PVP (polyvinylpyrrolidone) and carry out homogenate;
5, homogenate filters after stirring;
6, filtrate concentrates with ultra-fine filter, and the membrane retention molecular weight is 15000;
7, in filtrate, add antioxidant and stablizer;
8, last degerming, packing.
The SOD of method for preparing at room temperature can preserve 1 year.
Advantage of the present invention is: can extract SOD from corn, technology is simple, and is with short production cycle, and cost is low, and raw material sources are extensive, and use much cleaner, safety.Can be applicable to medicine, foods and cosmetics etc.
Most preferred embodiment of the present invention is as follows:
5000 gram corns were with 1% clorox surface sterilization 10 minutes, and water is rinsed well then.Cleaning the back steeped 48 hours in 28 ℃ of water loggings.Add 10 after the water logging, 000ml 50mm, PH7.8 phosphoric acid buffer add 500 gram PVP again, even dress in the high speed homogenizer.With homogenate stirring and leaching 1 hour at room temperature, to filter with membrane type inflation sheet frame squeezing machine, filtrate only is concentrated into two of original volume/a period of time with ultra-fine filter.Add sucrose and phosphoric acid salt in concentration process, last, degerming, packing are put into airtight, lucifuge, low temperature environment is preserved.
Above-mentioned sucrose can adopt gsh to substitute, and phosphoric acid salt can adopt Sodium Benzoate to substitute.
Claims (1)
1, a kind of method of from corn, extracting SOD, it is characterized in that: with raw material sterilization, washing, in 20-30 ℃ of water temperature, soaked 20-60 hour then, add 10000ml, 50mM, PH7.8 phosphoric acid buffer and 500 gram PVP subsequently and carry out homogenate, homogenate is stirring and leaching 1 hour at room temperature, filter again, ultrafiltration, concentrate, add antioxidant and stablizer afterwards, last degerming, packing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93115908A CN1040657C (en) | 1993-11-12 | 1993-11-12 | Method of extracting superoxide dismutase (SOD) from corn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93115908A CN1040657C (en) | 1993-11-12 | 1993-11-12 | Method of extracting superoxide dismutase (SOD) from corn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1087678A CN1087678A (en) | 1994-06-08 |
| CN1040657C true CN1040657C (en) | 1998-11-11 |
Family
ID=4991438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93115908A Expired - Fee Related CN1040657C (en) | 1993-11-12 | 1993-11-12 | Method of extracting superoxide dismutase (SOD) from corn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1040657C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1303208C (en) * | 2003-07-23 | 2007-03-07 | 韩伯钧 | Extraction method for SOD complex enzyme coarse powder from corn |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060213C (en) * | 1999-01-07 | 2001-01-03 | 王元志 | Method for large scale production of corn superoxide dismutase (SOD) complex enzyme |
| CN101892204B (en) * | 2010-06-18 | 2012-07-04 | 李作红 | Method for preparing corn superoxide dismutase from corn |
| CN105961003B (en) * | 2016-05-30 | 2019-03-12 | 路平 | A kind of implantation methods of the multienzyme crops containing SOD, multienzyme liquid containing SOD and preparation method thereof |
-
1993
- 1993-11-12 CN CN93115908A patent/CN1040657C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1303208C (en) * | 2003-07-23 | 2007-03-07 | 韩伯钧 | Extraction method for SOD complex enzyme coarse powder from corn |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1087678A (en) | 1994-06-08 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |