CN104062260B - A kind of containing the near infrared online detection method in naringin Chinese Traditional Medicine - Google Patents
A kind of containing the near infrared online detection method in naringin Chinese Traditional Medicine Download PDFInfo
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Abstract
The present invention provides a kind of containing the near infrared online detection method in naringin Chinese Traditional Medicine, and it comprises the steps: collect containing the sample in naringin Chinese Traditional Medicine and gather its near infrared spectrum；Prior art is utilized to measure the naringin content in sample；The naringin content that near infrared spectrum according to described sample and prior art record sets up detection model by chemo metric software, and it is 1100～2300nm that the spectral region of near infrared spectrum is chosen in the foundation of described detection model；Gather testing sample, utilize described detection model to measure naringin content and collection of illustrative plates similarity.The present invention provide containing the near infrared online detection method in naringin Chinese Traditional Medicine, can effectively solve containing mass analysis method efficiency in naringin Chinese Traditional Medicine low, the problem that wastage of material is too much.
Description
Technical field
The invention belongs to Chinese medicine extraction and preparation technique field, be specifically related to a kind of containing the production of naringin Chinese medicine
During near infrared online detection method.
Background technology
The production of compound Chinese medicinal preparation is a complex fabrication process process, different material base in production process,
The change of various technological parameters all may result in final unstable product quality.Quality control in production process
Rely primarily on experience and conventional mass analysis method, lack the effective real-time monitoring means of index component content.
In actual production, extraction time is fixed often, does not the most consider raw medicinal material mass discrepancy and fluctuation of operating conditions
The extraction terminal caused shifts to an earlier date or delayed, easily causes the instability of different batches extracting solution quality, causes Chinese medicine
Mass discrepancy between lot, and the energy, waste of time, the reduction etc. of medical material utilization rate.The reddest
It is fast, to sample nondestructive that external spectrum analytical technology (near infrared spectroscopy, NIRS) has analysis speed
Evil, without remarkable advantages such as chemical contaminations, be widely used in the raw material (master of crude drug at field of medicaments in recent years
Want active component, crystalline state, particle diameter, optical activity and density, differentiate the true and false of Chinese crude drug, the place of production and product
Matter classification), the course of processing (mixing uniformity, dry run moisture, injection product sterilizing, film clothing thickness,
In particle diameter, dissolution, medicine microorganism qualitative, quantitative monitoring) and final products (main component, hardness,
The qualification of packaging material, stability, authenticity) etc..
At present containing naringin Chinese Traditional Medicine lacking active component content quick nondestructive assay method, no
The quality Control of critical process link can be solved, cause being difficult to from the source of product solve silverblue and adjust fat
Capsule technologies and the stability of composition, integrity problem.
Summary of the invention
The technical problem to be solved is to overcome the defect of prior art, it is provided that a kind of containing Pericarpium Citri grandis
Near infrared online detection method in glycosides Chinese Traditional Medicine.
The present invention provides a kind of containing the near infrared online detection method in naringin Chinese Traditional Medicine, its bag
Include following steps:
Collect containing the sample in naringin Chinese Traditional Medicine and gather its near infrared spectrum；
Prior art is utilized to measure the naringin content in sample；
The naringin content that near infrared spectrum according to described sample and prior art record sets up detection model,
Described detection model includes that quantitative model and qualitative model, described quantitative model are set up by partial least square method,
The spectral region choosing infrared spectrum is 1100～2300nm, and described qualitative model is by TQ Analyst
Collection of illustrative plates similarity technology is set up, and the spectral region choosing infrared spectrum is 1100～2500nm；
Gather testing sample, utilize described detection model to measure naringin content.
What the present invention provided can efficient solution containing the near infrared online detection method in naringin Chinese Traditional Medicine
Certainly low containing mass analysis method efficiency in naringin Chinese Traditional Medicine, that wastage of material is too much problem.Receive
Collection different time points extract the concentrated solution in the extracting solution of process, concentration process, alcohol deposit fluid during precipitate with ethanol,
Mixed-powder during batch mixing, uses the sample near infrared spectrum of near infrared spectrometer scanning collection respectively；
Use known method to measure its quality index content, and in this, as reference value, pass through chemo metric software
Near infrared spectrum data is carried out pretreatment, sets up the near infrared spectrum on-line checking model of each production process,
By scanning the near infrared spectrum of each production process testing sample, then it is defeated to extract characteristic light spectrogram through computer
Enter to quantitative or qualitative model, the quality index content of the most measurable testing sample or the uniformity of material.Whole
Individual process time is short, speed is fast, accuracy is high, it is possible to realizes real-time online detection, is conducive to containing Pericarpium Citri grandis
The Whole Process Control that glycosides Chinese medicine produces, reduces production run cost, improves the product matter containing naringin Chinese medicine
Amount stability.Along with to the further investigation of PLS method and application, by ad hoc approach screening characteristic wavelength or
Range of wavelengths can obtain more preferable quantitative calibration models.Wavelength selects on the one hand can be main with simplified model
It is due to the rejecting of uncorrelated or non-linear variable, the straightening die that predictive ability is strong, robustness is good can be obtained
Type.
Accompanying drawing explanation
Fig. 1 is the flow chart that the embodiment of the present invention contains naringin Chinese Traditional Medicine；
Fig. 2 is that the near infrared online detection model that the embodiment of the present invention contains in naringin Chinese Traditional Medicine is built
Vertical flow chart schematic diagram；
Fig. 3 is that process sample naringin content scattergram is decocted in the embodiment of the present invention 1 silverblue blood fat regulating capsule extraction one；
Fig. 4 is that process sample naringin content scattergram is decocted in the embodiment of the present invention 1 silverblue blood fat regulating capsule extraction two；
Fig. 5 is the embodiment of the present invention 1 silverblue blood fat regulating capsule concentration process sample naringin content scattergram；
Fig. 6 is that the embodiment of the present invention 1 silverblue blood fat regulating capsule reclaims ethanol process sample naringin content scattergram；
Fig. 7 is the embodiment of the present invention 1 silverblue blood fat regulating capsule material mixed process sample near infrared spectrum similarity
With incorporation time scattergram；
Fig. 8 is that the embodiment of the present invention 1 silverblue blood fat regulating capsule extracts a near infrared spectrum superposition decocting process sample
Figure；
Fig. 9 is that the embodiment of the present invention 1 silverblue blood fat regulating capsule extracts the two near infrared spectrum superpositions decocting process sample
Figure；
Figure 10 is the near infrared spectrum stacking chart of the embodiment of the present invention 1 silverblue blood fat regulating capsule concentration process sample；
Figure 11 is the near infrared spectrum superposition that the embodiment of the present invention 1 silverblue blood fat regulating capsule reclaims ethanol process sample
Figure；
Figure 12 be the embodiment of the present invention 1 silverblue blood fat regulating capsule extract one decoct the naringin content predictive value of sample with
Relevant figure between measured value；
Figure 13 be the embodiment of the present invention 1 silverblue blood fat regulating capsule extract two decoct the naringin content predictive values of sample with
Relevant figure between measured value；
Figure 14 be the embodiment of the present invention 1 silverblue blood fat regulating capsule concentration process sample naringin content predictive value with
Relevant figure between measured value；
Figure 15 is the naringin content prediction that the embodiment of the present invention 1 silverblue blood fat regulating capsule reclaims ethanol process sample
It is worth and the relevant figure between measured value；
Figure 16 is that the embodiment of the present invention 1 silverblue blood fat regulating capsule material mixed process sample point each sweep time is the reddest
External spectrum standard deviation stacking chart.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and reality
Execute example, the present invention is further elaborated.Only should be appreciated that specific embodiment described herein
Only in order to explain the present invention, it is not intended to limit the present invention.
The embodiment of the present invention provides a kind of containing the near infrared online detection side in naringin Chinese Traditional Medicine
Method, it comprises the steps:
S01: collect containing the sample in naringin Chinese Traditional Medicine and gather its near infrared spectrum；
S02: utilize prior art to measure the naringin content in sample；
S03: set up detection according to the naringin content that near infrared spectrum and the prior art of described sample record
Model, described detection model includes that quantitative model and qualitative model, described quantitative model pass through offset minimum binary
Method is set up, and the spectral region choosing infrared spectrum is 1100～2300nm, and described qualitative model passes through TQ
In Analyst, collection of illustrative plates similarity technology is set up, and the spectral region choosing infrared spectrum is 1100～2500nm；
S04: gather testing sample, utilizes described detection model to measure naringin content.
In step S01, stopwatch times is consistent with machine time furnishing, open soft when technical process starts
Part Automatic continuous gathers spectrum；During sampling, when flowing out with effective sample, sample valve valve opening time record
For initial time, with the valve closing time for terminating the time, time period is sample time；The sample obtained
Corresponding spectrum is the spectrum that continuous acquisition obtains in time period.Preferably, in each step of technical process
All carry out sample collecting.As a example by silverblue blood fat regulating capsule, the production technology of silverblue blood fat regulating capsule is as shown in Figure 1.
In the production process of described silverblue blood fat regulating capsule sample collecting be collect containing in naringin Chinese Traditional Medicine not
Extracting solution, concentrated solution, ethanol recovered liquid and mixture samples with time point.The extraction of described different time points
The extracting solution of each time point during liquid refers to silverblue blood fat regulating capsule first time, decoction is extracted for the second time；Different
The concentrated solution of time point refers to the concentrated solution of each time point in silverblue blood fat regulating capsule concentration process；Different time points
Ethanol recovered liquid refer to that silverblue blood fat regulating capsule alcohol deposit fluid reclaims the recovered liquid of each time point in ethanol process；No
The batch mixing of each time point during silverblue blood fat regulating capsule batch mixing is referred to the batch mixing of time point.
Step S02 is specifically, the naringin content naringin content that prior art measures in sample preferably employs
High effective liquid chromatography for measuring, chromatographic column: Aglient Extend-C18,4.6 × 250mm, 5 μm posts；With
Methanol-acetic acid-water (35:4:61) is flowing phase；Detection wavelength: 283nm；Flow velocity: 1.0mL/min；Post
Temperature: 25 DEG C.The preparation of reference substance solution: take naringin reference substance appropriate, accurately weighed, add methanol and make
Every 1mL solution containing naringin 61 μ g, to obtain final product.Take suitable containing the sample in naringin Chinese Traditional Medicine
Amount is diluted with water to containing naringin about 19.68～98.40mg/L.Respectively precision draws reference substance solution and dilute
The each 10 μ L of solution after releasing, inject chromatograph of liquid and are measured, obtain the content of naringin.
In step S03, set up quantitative model by partial least square method (PLS).Modeling parameters: sample
With original NIRS spectrum, modeling wave band is 1100～2300nm.For avoiding the occurrence of " over-fitting " phenomenon,
Need number of principal components is rationally selected.In concrete modeling process, preferably the reddest to collect
External spectrum makees pretreatment.The preprocess method such as multiplicative scatter correction (MSC) of routine, normal orthogonal variable
Conversion (SNV), first differential, second-order differential, S-G be smooth and Norris derivative filtering etc. all can make
With, it is possible to do not make pretreatment.For routine contain naringin Chinese Traditional Medicine be often divided into extraction, concentration,
Reclaim ethanol, material mixed process.Each production process is set up quantitatively or qualitative model.As silverblue adjusts fat
During capsule manufacture, the parameter of decoction extraction process naringin quantitative model for the first time: spectral range
1100～2300nm, original spectrum is without process, number of principal components 10, rejecting abnormalities point；Second time decocts and carries
Take the parameter of process naringin quantitative model: spectral range 1100～2300nm, original spectrum is with first derivative
Smooth with Norris and carry out pretreatment, number of principal components 4, rejecting abnormalities point；The quantitative mould of concentration process naringin
The parameter of type: spectral range 1100～2300nm, original spectrum carries out pre-place so that second dervative and SG are smooth
Reason, number of principal components 11, rejecting abnormalities point；The parameter of recovery ethanol process naringin quantitative model: wave spectrum model
Enclosing 1100～2300nm, original spectrum carries out pretreatment, number of principal components 10 with multiplicative scatter correction, rejects
Abnormity point.The foundation of batch mixing process qualitative model: collection of illustrative plates similarity technology in application TQ Analyst
(Similarity match) sets up qualitative model.Modeling parameters: sample NIRS spectrum with first derivative and
Norris is smooth carries out pretreatment, models wave band 1000～2500nm.
The embodiment of the present invention utilizes near-infrared spectrum technique to combine chemo metric software, sets up containing naringin
Online quickly naringin in each production process of Chinese medicine (extract, concentrate, reclaim ethanol, material mixed process)
Quantitatively or qualitative detection model, improve quality testing efficiency in production process with this, final ensure containing Pericarpium Citri grandis
The stability of glycosides tcm product quality.
Below in conjunction with specific embodiment the present invention implemented and be described in detail.
Embodiment 1:
Near infrared spectrometer selected by the present embodiment is that Brimrose company of U.S. Luminar3060 acousto-optic can
Adjusting On-line NIR instrument (numbering 1112-SP-10979), critical piece includes: opticator, control
Partly, the part such as 675 transflector probes, DN25 fluid sample measuring device, optical fiber composition.Detector used by instrument
For InGaAs.The present embodiment extract in silverblue blood fat regulating capsule production process, concentrate, precipitate with ethanol, recovery ethanol
Equipment is Guangzhou Hanfang Pharmaceutical Co., Ltd.'s automation equipment.Material mixed process uses
Nicolet6700 Fourier transformation infrared spectrometer (Thermo Nicolet company of the U.S.), is furnished with integrating sphere
Diffuse-reflectance sampling system and Omnic signals collecting software.The present embodiment further relates to Agilent1200 high-efficient liquid
Chromatography, Mettler XS205DU type electronic analytical balance (Switzerland).The data of the present embodiment process
Software is CAMO Unscrambler9.7 and TQ Analyst8.0 software.
It is biological that the naringin reference substance (lot number: 110722-200610) that the present embodiment uses is purchased from China's medicine
Goods examine and determine institute；Methanol is chromatographically pure, and liquid phase water is distilled water, and remaining reagent is analytical pure.
Method and result
Sampling and the collection of near infrared spectrum
As a example by embodiment of the present invention near infrared online detection in silverblue blood fat regulating capsule production process.Silverblue is adjusted
Fat capsule is mainly made up of Exocarpium Citri Grandis, Folium Ginkgo, Herb Gynostemmae Pentaphylli and propolis, have invigorating the spleen and replenishing QI, blood circulation promoting and blood stasis dispelling,
Effect of drying dampness to eliminate phlegm, is mainly used in treating qi-asthenia phlegm stasis blocking numbness type hyperlipemia.Research shows, silverblue is adjusted
In fat capsule one of flavones ingredient naringin blood fat reducing, calmness, antioxidation, antitumor, antifungal,
Atherosclerosis, spasmolytic, ease pain, regulate the aspects such as blood glucose there is stronger biological activity.
Silverblue blood fat regulating capsule production process produces according to the flow process of Fig. 1.By stopwatch times and machine time
Furnishing is consistent, opens software Automatic continuous when technical process starts and gathers spectrum；During sampling, with effective sample
When product flow out, sampling valve valve opening time is recorded as initial time, with the valve closing time for terminating the time,
Time period is sample time；The sample correspondence spectrum obtained is the spectrum that continuous acquisition obtains in time period.
Extraction one collects 153 parts of samples during decocting altogether, selects 137 parts of representative sample composition
Calibration set, remaining 16 parts of sample composition checking collection.Extraction two collects 163 parts of samples during decocting altogether, chooses
Select 147 parts of composition calibration sets of representative sample, remaining 16 parts of sample composition checking collection.Concentration process
In collect 129 parts of samples altogether, select 116 parts of composition calibration sets of representative sample, remaining is 13 years old
Part sample composition checking collection.Reclaim in ethanol process and collect 102 parts of samples altogether, select representative
92 parts of composition calibration sets of sample, remaining 10 parts of sample composition checking collection.Material mixed process is every 1min
Scan 1 time, scanning 16 times altogether.Embodiment is extracted one decoct, two decoct, concentrate, reclaim ethanol, material mixes
Fig. 3～Fig. 7 is shown in the near infrared spectrum superposition closing process sample.
HPLC measures the naringin content of sample in each production process of silverblue blood fat regulating capsule
Chromatographic condition
Chromatographic column: Aglient Extend-C18,4.6mm × 250mm, 5 μm posts；With methanol-acetic acid-water
(35:4:61) for flowing phase；Detection wavelength: 283nm；Flow velocity: 1.0mL/min；Column temperature: 25 DEG C；
The preparation of reference substance solution
Take naringin reference substance appropriate, accurately weighed, add methanol and make molten containing naringin 61 μ g of every 1mL
Liquid, to obtain final product；
The preparation of need testing solution
Take each production process sample of silverblue blood fat regulating capsule to be placed in right amount in 25mL volumetric flask, add distilled water extremely
Scale, to obtain final product.
Algoscopy
Precision draws reference substance solution and each 10 μ L of need testing solution respectively, injects chromatograph of liquid and surveys
Fixed, obtain the content of naringin.
Embodiment is extracted one decoct, the two content distribution figures decocting, concentrate, reclaim ethanol process sample naringin,
See Fig. 8～Figure 11.
The foundation of model
Extract the foundation decocting naringin quantitative model
Application PLS method sets up quantitative model.Modeling parameters: sample, with original NIRS spectrum, models wave band
1100～2300nm, number of principal components 10, rejecting abnormalities point, must extract one through cross validation and decoct naringin calmly
Amount model rC=0.9919, RMSEC=2.5396；rP=0.9565, RMSEP=5.8424；Model is pre-
Relevant figure between measured value to measured value is shown in Figure 12.
Extract two foundation decocting naringin quantitative model
Application PLS method sets up quantitative model.Modeling parameters: sample, with original NIRS spectrum, models wave band
1100～2300nm, number of principal components 4, rejecting abnormalities point, must extracting two through cross validation, to decoct naringin quantitative
Model rC=0.9747, RMSEC=0.9522；rP=0.9421, RMSEP=1.4290；Model prediction
Relevant figure between value to measured value is shown in Figure 13.
The foundation of concentration process naringin quantitative model
Application PLS method sets up quantitative model.Modeling parameters: sample NIRS spectrum is put down with second dervative+SG
Slide into row pretreatment, model wave band 1100～2300nm, number of principal components 11, rejecting abnormalities point.Through intersecting
Verify to obtain concentration process naringin quantitative model rC=0.9995, RMSEC=9.8837；rP=0.9918,
RMSEP=39.6042；Relevant figure between model predication value to measured value is shown in Figure 14.
Reclaim the foundation of ethanol process naringin quantitative model
Application PLS method sets up quantitative model.Modeling parameters: sample NIRS spectrum MSC carries out pretreatment,
Modeling wave band is 1100～2300nm, number of principal components 9, rejecting abnormalities point.Second must be reclaimed through cross validation
Alcohol process naringin quantitative model rC=0.9942, RMSEC=70.6075；rP=0.9891, RMSEP=
97.3434；Relevant figure between model predication value to measured value is shown in Figure 15.
The foundation of material mixed process qualitative model
In application TQ Analyst8.0, collection of illustrative plates similarity technology (Similarity match) sets up qualitative model.
Modeling parameters: sample NIRS spectrum carries out pretreatment so that first derivative+Norris is smooth, modeling wave band 1000～
2500nm.Material mixed process different sample point profile criteria deviation stacking charts per minute see Figure 16.
According to above-mentioned set up calibration model, the near infrared spectrum data of checking collection sample is imported in model,
Can fast prediction naringin content.Result shows, inclined between NIRS predictive value and HPLC measured value
Difference is less, and the predictive ability of institute's established model is preferable, is shown in Table 1.
The checking of table 1 silverblue blood fat regulating capsule production process near-infrared model
Table 2 silverblue blood fat regulating capsule material mixed process
From the experimental result in table 1, table 2, set up containing in naringin Chinese Traditional Medicine
Near infrared online detection method is easy, quick, accurate, highly sensitive, precision, good stability.The reddest
After outer forecast model is successfully established, the content of unknown sample naringin can be measured rapidly and accurately, the suitableeest
Fast mass for each production process of silverblue blood fat regulating capsule detects, thus improves silverblue blood fat regulating capsule quality
Stability, it is ensured that its clinical efficacy.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (4)
1. the near infrared online detection method contained in naringin Chinese Traditional Medicine, it is characterised in that
Comprise the steps:
Collect containing the sample in naringin Chinese Traditional Medicine and gather its near infrared spectrum, described containing Fructus Citri grandis
Skin glycosides Chinese Traditional Medicine includes extracting, concentrates, reclaims ethanol, material mixed process；
Prior art is utilized to measure the naringin content in sample；
The naringin content that near infrared spectrum according to described sample and prior art record sets up detection model,
Described detection model includes that quantitative model and qualitative model, described quantitative model are set up by partial least square method,
The spectral region choosing infrared spectrum is 1100～2300nm, and described qualitative model is soft by TQ Analyst
Collection of illustrative plates similarity technology in part is set up, and the spectral region choosing infrared spectrum is 1100～2500nm；
Gather testing sample, utilize described detection model to measure naringin content；
Wherein, described is silverblue blood fat regulating capsule containing naringin Chinese medicine；
In the production process of described silverblue blood fat regulating capsule, sample collecting is to collect containing naringin Chinese Traditional Medicine
The extracting solution of middle different time points, concentrated solution, ethanol recovered liquid and mixture samples；
The extracting solution of described different time points refers to that silverblue blood fat regulating capsule decocts extraction process for the first time, for the second time
In the extracting solution of each time point；When the concentrated solution of different time points refers to each in silverblue blood fat regulating capsule concentration process
Between point concentrated solution；The ethanol recovered liquid of different time points refers to that silverblue blood fat regulating capsule alcohol deposit fluid reclaims ethanol mistake
The recovered liquid of each time point in journey；When the batch mixing of different time points is each during referring to silverblue blood fat regulating capsule batch mixing
Between point batch mixing；
The parameter of decoction of described first time extraction process naringin quantitative model: spectral range 1100～2300
Nm, original spectrum is without process, number of principal components 10, rejecting abnormalities point；Described second time decocts extraction process Fructus Citri grandis
The parameter of skin glycosides quantitative model: spectral range 1100～2300nm, original spectrum is with first derivative and Norris
Smooth and carry out pretreatment, number of principal components 4, rejecting abnormalities point；Described concentration process naringin quantitative model
Parameter: spectral range 1100～2300nm, original spectrum carries out pretreatment so that second dervative and SG are smooth,
Number of principal components 11, rejecting abnormalities point；The parameter of described recovery ethanol process naringin quantitative model: wave spectrum model
Enclosing 1100～2300nm, original spectrum carries out pretreatment, number of principal components 10 with multiplicative scatter correction, rejects
Abnormity point；The parameter of described batch mixing process qualitative model: spectral range 1000～2500nm, original spectrum
Pretreatment is carried out so that first derivative and Norris are smooth.
The most according to claim 1 containing the near infrared online detection side in naringin Chinese Traditional Medicine
Method, it is characterised in that described prior art is high performance liquid chromatography.
The most according to claim 1 containing the near infrared online detection side in naringin Chinese Traditional Medicine
Method, it is characterised in that described collection containing the sample in naringin Chinese Traditional Medicine be divided into calibration set and
Checking collection, sets up calibration set respectively and prior art measures the corresponding relation between the naringin content in sample,
Detection model is verified by recycling checking collection sample, optimizes containing the inspection in naringin Chinese Traditional Medicine
Survey model.
4. according to arbitrary described the existing containing the near-infrared in naringin Chinese Traditional Medicine of claims 1 to 3
Line detecting method application in Chinese medicine quality monitors.
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| CN105234094B (en) * | 2015-08-27 | 2017-12-22 | 威海御膳坊生物科技有限公司 | Using the capsule real-time inspection platform of double check |
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| CN106770001A (en) * | 2016-11-11 | 2017-05-31 | 本溪国家中成药工程技术研究中心有限公司 | Method and application using extract solution concentration process in the preparation process of near infrared spectroscopy quick detection qizhi weitong granules |
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| CN108918460A (en) * | 2018-09-30 | 2018-11-30 | 福建医科大学 | A method of based on aristolochic acid I content in near-infrared spectrum technique measurement Chinese medicine |
| CN111024869A (en) * | 2019-09-26 | 2020-04-17 | 天圣制药集团股份有限公司 | Near-infrared quality detection system for ginkgo leaf purification process of capsule containing radix codonopsis pilosulae and dredging collaterals |
| CN110665842A (en) * | 2019-11-14 | 2020-01-10 | 广西立盛茧丝绸有限公司 | Near infrared spectrum cocoon selection method |
| CN110987865A (en) * | 2019-12-13 | 2020-04-10 | 齐鲁工业大学 | Method for detecting fig quality based on near infrared spectrum |
| CN113155572A (en) * | 2021-04-28 | 2021-07-23 | 广州科曼生物科技有限公司 | Pummelo peel foetus control extract and preparation method and application thereof |
| CN113484272B (en) * | 2021-07-08 | 2022-08-19 | 云南中烟工业有限责任公司 | Method for rapidly predicting oil content in fresh tobacco leaves by adopting similarity analysis technology based on near infrared spectrum |
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