CN104062260A - Near-infrared on-line detection method used in production process of traditional Chinese medicine containing naringin - Google Patents
Near-infrared on-line detection method used in production process of traditional Chinese medicine containing naringin Download PDFInfo
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Abstract
The invention provides a near-infrared on-line detection method used in production process of a traditional Chinese medicine containing naringin. The method comprises the following steps: collecting a sample in the production process of the traditional Chinese medicine containing naringin, and acquiring a near-infrared spectrum of the sample; measuring the content of naringin in the sample by using the existing technology; establishing a detection model by using chemometrics software according to the near-infrared spectrum of the sample and the naringin content measured by using the existing technology, wherein the spectrum range of the near-infrared spectrum of the detection model is 1100-2300nm; and acquiring the sample to be detected, and measuring the content of naringin and the spectrum similarity degree by using the detection model. By adopting the near-infrared on-line detection method used in the production process of the traditional Chinese medicine containing naringin, the problems that a quality analysis method is low in efficiency and too many raw materials are wasted in the production process of the traditional Chinese medicine containing naringin are effectively solved.
Description
Technical field
The invention belongs to traditional Chinese medicine extraction and preparation technique field, be specifically related to a kind of near infrared online detection method containing in aurantiin Chinese Traditional Medicine.
Background technology
The production of compound Chinese medicinal preparation is a complex fabrication process process, and in production run, the variation former, various technological parameters of different material base all can cause final unstable product quality.Quality control in production run mainly relies on experience and conventional mass analysis method, lacks effective index component content Real-Time Monitoring means.In actual production, extraction time is fixed often, substantially do not consider that the extraction terminal that raw medicinal material mass discrepancy and fluctuation of operating conditions cause shifts to an earlier date or lags behind, easily cause the unstable of different batches extract quality, cause the mass discrepancy between Chinese medicine lot, and the energy, waste of time, the reduction of medicinal material utilization factor etc.Near-infrared spectral analysis technology (near infrared spectroscopy, NIRS) there is analysis speed fast, to sample nondestructive evil, without remarkable advantages such as chemical contaminations, be widely used in the raw material (main active of bulk drug at field of medicaments in recent years, crystalline state, particle diameter, optical activity and density, differentiate the true and false of Chinese crude drug, the place of production and quality grading), process (mixing uniformity, dry run moisture, the sterilizing of injection product, film clothing thickness, particle diameter, dissolution rate, microorganism qualitative, quantitative monitoring in medicine) and final products (principal ingredient, hardness, the qualification of wrappage, stability, authenticity) etc.
Contain at present and in aurantiin Chinese Traditional Medicine, lack active component content quick nondestructive assay method, can not solve the quality Control of critical process link, cause being difficult to solve from the source of product stability, the integrity problem of Beijing enamel ware blood fat regulating capsule technique and composition.
Summary of the invention
Technical matters to be solved by this invention is to overcome the defect of prior art, and a kind of near infrared online detection method containing in aurantiin Chinese Traditional Medicine is provided.
The invention provides a kind of near infrared online detection method containing in aurantiin Chinese Traditional Medicine, it comprises the steps:
Collection contains the sample in aurantiin Chinese Traditional Medicine and gathers its near infrared spectrum;
Utilize the naringin content in prior art working sample;
The naringin content recording according to the near infrared spectrum of described sample and prior art is set up detection model, described detection model comprises quantitative model and qualutative model, described quantitative model is set up by partial least square method, the spectral range of choosing infrared spectrum is 1100～2300nm, described qualutative model is set up by collection of illustrative plates similarity technology in TQ Analyst, and the spectral range of choosing infrared spectrum is 1100～2500nm;
Gather testing sample, utilize described detection model to measure naringin content.
The near infrared online detection method containing in aurantiin Chinese Traditional Medicine provided by the invention can effectively solve that to contain in aurantiin Chinese Traditional Medicine mass analysis method efficiency low, the problem that wastage of material is too much.Mixed-powder in concentrate in extract, the concentration process of collection different time points leaching process, precipitation solution, the batch mixing process in alcohol precipitation process, uses the near infrared spectrometer sample near infrared spectrum of scanning collection respectively; Adopt known method to measure its quality index content, and using this as reference value, by Chemical Measurement software, near infrared spectrum data is carried out to pre-service, set up the online detection model of near infrared spectrum of each production run, by scanning the near infrared spectrum of each production run testing sample, extracting characteristic light spectrogram through computing machine is again input to quantitatively or qualutative model, i.e. the quality index content of measurable testing sample or the uniformity coefficient of material.Whole process time is short, speed is fast, accuracy is high, can realize real-time online and detect, and is conducive to contain the Whole Process Control that aurantiin Chinese medicine is produced, and reduces production run cost, improves the constant product quality that contains aurantiin Chinese medicine.Along with the further investigation to PLS method and application, screen characteristic wavelength or range of wavelengths can obtain better quantitative correction model by ad hoc approach.Wavelength is selected on the one hand can simplified model, and main is the rejecting due to uncorrelated or non-linear variable, can obtain the calibration model that predictive ability is strong, robustness is good.
Brief description of the drawings
Fig. 1 is the process flow diagram that the embodiment of the present invention contains aurantiin Chinese Traditional Medicine;
Fig. 2 is that the embodiment of the present invention contains the near infrared online detection model Establishing process figure schematic diagram in aurantiin Chinese Traditional Medicine;
Fig. 3 is that process sample naringin content distribution plan is decocted in the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extraction one;
Fig. 4 is that process sample naringin content distribution plan is decocted in the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extraction two;
Fig. 5 is the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule concentration process sample naringin content distribution plan;
Fig. 6 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule reclaims ethanol process sample naringin content distribution plan;
Fig. 7 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule mixing of materials process sample near infrared spectrum similarity is with incorporation time distribution plan;
Fig. 8 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extracts a near infrared spectrum stacking diagram who decocts process sample;
Fig. 9 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extracts the two near infrared spectrum stacking diagrams that decoct process sample;
Figure 10 is the near infrared spectrum stacking diagram of the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule concentration process sample;
Figure 11 is the near infrared spectrum stacking diagram that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule reclaims ethanol process sample;
Figure 12 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extracts a correlogram of decocting between naringin content predicted value and the measured value of sample;
Figure 13 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule extracts two correlograms of decocting between naringin content predicted value and the measured value of sample;
Figure 14 is the correlogram between naringin content predicted value and the measured value of the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule concentration process sample;
Figure 15 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule reclaims the correlogram between naringin content predicted value and the measured value of ethanol process sample;
Figure 16 is that the embodiment of the present invention 1 Beijing enamel ware blood fat regulating capsule mixing of materials process sample is put near infrared spectrum standard deviation stacking diagram each sweep time.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the present invention provides a kind of near infrared online detection method containing in aurantiin Chinese Traditional Medicine, and it comprises the steps:
S01: collect and contain the sample in aurantiin Chinese Traditional Medicine and gather its near infrared spectrum;
S02: utilize the naringin content in prior art working sample;
S03: the naringin content recording according to the near infrared spectrum of described sample and prior art is set up detection model, described detection model comprises quantitative model and qualutative model, described quantitative model is set up by partial least square method, the spectral range of choosing infrared spectrum is 1100～2300nm, described qualutative model is set up by collection of illustrative plates similarity technology in TQAnalyst, and the spectral range of choosing infrared spectrum is 1100～2500nm;
S04: gather testing sample, utilize described detection model to measure naringin content.
In step S01, by consistent with machine time furnishing the stopwatch time, in the time that technological process starts, open software Automatic continuous and gather spectrum; When sampling, while outflow with effective sample, sample valve valve opening time is recorded as initial time, and taking the valve closing time as the termination time, this time period is sample time; The corresponding spectrum of the sample spectrum that interior continuous acquisition of time period obtains for this reason of obtaining.Preferably, carry out sample collection in each step of technological process.Taking Beijing enamel ware blood fat regulating capsule as example, the production technology of Beijing enamel ware blood fat regulating capsule as shown in Figure 1.In the production run of described Beijing enamel ware blood fat regulating capsule, sample collection is to collect the extract, concentrate, the ethanol that contain different time points in aurantiin Chinese Traditional Medicine to reclaim liquid and mixture samples.The extract of described different time points refers to that Beijing enamel ware blood fat regulating capsule decocts the extract of each time point in leaching process for the first time, for the second time; The concentrate of different time points refers to the concentrate of each time point in Beijing enamel ware blood fat regulating capsule concentration process; The ethanol of different time points reclaims liquid and refers to that Beijing enamel ware blood fat regulating capsule precipitation solution reclaims the recovery liquid of each time point in ethanol process; The batch mixing of different time points refers to the batch mixing of each time point in Beijing enamel ware blood fat regulating capsule batch mixing process.
Step S02 is specially, and the naringin content naringin content in prior art working sample preferably adopts high effective liquid chromatography for measuring, chromatographic column: Aglient Extend-C18,4.6 × 250mm, 5 μ m posts; Taking methyl alcohol-Acetic Acid-Water (35:4:61) as mobile phase; Detect wavelength: 283nm; Flow velocity: 1.0mL/min; Column temperature: 25 DEG C.The preparation of reference substance solution: get aurantiin reference substance appropriate, accurately weighed, add methyl alcohol and make the solution of every 1mL containing aurantiin 61 μ g, to obtain final product.Containing sample in aurantiin Chinese Traditional Medicine gets to be diluted with water in right amount and contains approximately 19.68～98.40mg/L of aurantiin.The accurate each 10 μ L of solution that draw after reference substance solution and dilution respectively, injection liquid chromatography is measured, and obtains the content of aurantiin.
In step S03, set up quantitative model by partial least square method (PLS).Modeling parameters: sample is with original NIRS spectrum, and modeling wave band is 1100～2300nm.For avoiding occurring " over-fitting " phenomenon, need to carry out choose reasonable to number of principal components.In concrete modeling process, preferably the near infrared spectrum collecting is done to pre-service.Conventional preprocess method, such as polynary scatter correction (MSC), the conversion of standard orthogonal variable (SNV), single order differential, second-order differential, S-G are level and smooth and Norris derivative filtering etc. all can be used, also can not done pre-service.Containing aurantiin Chinese Traditional Medicine for routine is often divided into extraction, concentrates, reclaims ethanol, mixing of materials process.Each production run is set up quantitatively or qualutative model.In Beijing enamel ware blood fat regulating capsule production run, decoct for the first time the parameter of leaching process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum non-processor, number of principal components 10, rejecting abnormalities point; Decoct for the second time the parameter of leaching process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum smoothly carries out pre-service with first order derivative and Norris, number of principal components 4, rejecting abnormalities point; The parameter of concentration process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum smoothly carries out pre-service with second derivative and SG, number of principal components 11, rejecting abnormalities point; Reclaim the parameter of ethanol process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum carries out pre-service with polynary scatter correction, number of principal components 10, rejecting abnormalities point.The foundation of batch mixing process qualutative model: in application TQ Analyst, collection of illustrative plates similarity technology (Similarity match) is set up qualutative model.Modeling parameters: sample NIRS spectrum smoothly carries out pre-service with first order derivative and Norris, modeling wave band 1000～2500nm.
The embodiment of the present invention utilizes near-infrared spectrum technique in conjunction with Chemical Measurement software, foundation contains in the each production run of aurantiin Chinese medicine (extract, concentrated, reclaim ethanol, mixing of materials process) online aurantiin fast quantitatively or qualitative detection model, improve quality testing efficiency in production run with this, the stability that final guarantee contains aurantiin tcm product quality.
Below in conjunction with specific embodiment, specific implementation of the present invention is described in detail.
Embodiment 1:
The selected near infrared spectrometer of the present embodiment is the adjustable On-line NIR instrument of the U.S.'s Luminar3060 of Brimrose company acousto-optic (numbering 1112-SP-10979), and critical piece comprises: the part such as opticator, control section, 675 saturating reflection probe, DN25 fluid sample measuring device, optical fiber composition.Instrument detecting device used is InGaAs.In the present embodiment, in Beijing enamel ware blood fat regulating capsule production run, extract, concentrated, alcohol precipitation, recovery ethanol equipment be Guangzhou Hanfang Pharmaceutical Co., Ltd.'s automation equipment.Mixing of materials process adopts Nicolet6700 Fourier transformation infrared spectrometer (Thermo Nicolet company of the U.S.), is furnished with integrating sphere diffuse reflection sampling system and Omnic signals collecting software.The present embodiment also relates to Agilent1200 high performance liquid chromatograph, Mettler XS205DU type electronic analytical balance (Switzerland).The data processing software of the present embodiment is CAMO Unscrambler9.7 and TQ Analyst8.0 software.
The aurantiin reference substance (lot number: 110722-200610) that the present embodiment uses is purchased from Nat'l Pharmaceutical & Biological Products Control Institute; Methyl alcohol is chromatographically pure, and liquid phase water is distilled water, and it is pure that all the other reagent are analysis.
Method and result
The collection of sampling and near infrared spectrum
The embodiment of the present invention is taking the near infrared online detection in Beijing enamel ware blood fat regulating capsule production run as example.Beijing enamel ware blood fat regulating capsule is mainly made up of Exocarpium Citri Grandis, ginkgo leaf, gynostemma pentaphylla and propolis, have invigorate the spleen and benefit qi, promoting blood circulation and removing blood stasis, eliminating dampness and eliminating phlegm effect, be mainly used in treating qi-asthenia phlegm stasis blocking numbness type hyperlipidemia.Research shows, in Beijing enamel ware blood fat regulating capsule, one of flavones ingredient aurantiin has stronger biologically active at aspects such as reducing blood lipid, calmness, anti-oxidant, antitumor, antimycotic, antiatherosclerosis, spasmolysis, analgesia, adjusting blood sugar.
Beijing enamel ware blood fat regulating capsule production run is produced according to the flow process of Fig. 1.By consistent with machine time furnishing the stopwatch time, in the time that technological process starts, open software Automatic continuous and gather spectrum; When sampling, while outflow with effective sample, sample valve valve opening time is recorded as initial time, and taking the valve closing time as the termination time, this time period is sample time; The corresponding spectrum of the sample spectrum that interior continuous acquisition of time period obtains for this reason of obtaining.
Extraction one is decocted in process and is collected altogether 153 duplicate samples, selects 137 parts of composition calibration sets of representative sample, all the other 16 duplicate samples composition checking collection.Extraction two is decocted in process and is collected altogether 163 duplicate samples, selects 147 parts of composition calibration sets of representative sample, all the other 16 duplicate samples composition checking collection.In concentration process, collect altogether 129 duplicate samples, select 116 parts of composition calibration sets of representative sample, all the other 13 duplicate samples composition checking collection.Reclaim in ethanol process and collect altogether 102 duplicate samples, select 92 parts of composition calibration sets of representative sample, all the other 10 duplicate samples composition checking collection.Mixing of materials process, every 1min scanning 1 time, scans 16 times altogether.In embodiment, extract one decoct, the two near infrared spectrums stacks of decocting, concentrate, reclaiming ethanol, mixing of materials process sample are shown in Fig. 3～Fig. 7.
HPLC measures the naringin content of sample in the each production run of Beijing enamel ware blood fat regulating capsule
Chromatographic condition
Chromatographic column: Aglient Extend-C18,4.6mm × 250mm, 5 μ m posts; Taking methyl alcohol-Acetic Acid-Water (35:4:61) as mobile phase; Detect wavelength: 283nm; Flow velocity: 1.0mL/min; Column temperature: 25 DEG C;
The preparation of reference substance solution
Get aurantiin reference substance appropriate, accurately weighed, add methyl alcohol and make the solution of every 1mL containing aurantiin 61 μ g, to obtain final product;
The preparation of need testing solution
Get each production run sample of Beijing enamel ware blood fat regulating capsule and be placed in right amount 25mL volumetric flask, adding distil water, to scale, to obtain final product.
Determination method
Accurate reference substance solution and the each 10 μ L of need testing solution of drawing respectively, injection liquid chromatography is measured, and obtains the content of aurantiin.
In embodiment, extract one decoct, the two content distribution figure that decoct, concentrate, reclaim ethanol process sample aurantiin, see Fig. 8～Figure 11.
The foundation of model
Extract a foundation of decocting aurantiin quantitative model
Application PLS method is set up quantitative model.Modeling parameters: sample is with original NIRS spectrum, modeling wave band 1100～2300nm, number of principal components 10, rejecting abnormalities point, must extract one through cross validation and decoct aurantiin quantitative model r
c=0.9919, RMSEC=2.5396; r
p=0.9565, RMSEP=5.8424; Correlogram between model predication value and measured value is shown in Figure 12.
Extract two foundation of decocting aurantiin quantitative model
Application PLS method is set up quantitative model.Modeling parameters: sample is with original NIRS spectrum, modeling wave band 1100～2300nm, number of principal components 4, rejecting abnormalities point, must extract two through cross validation and decoct aurantiin quantitative model r
c=0.9747, RMSEC=0.9522; r
p=0.9421, RMSEP=1.4290; Correlogram between model predication value and measured value is shown in Figure 13.
The foundation of concentration process aurantiin quantitative model
Application PLS method is set up quantitative model.Modeling parameters: sample NIRS spectrum smoothly carries out pre-service with second derivative+SG, modeling wave band 1100～2300nm, number of principal components 11, rejecting abnormalities point.Obtain concentration process aurantiin quantitative model r through cross validation
c=0.9995, RMSEC=9.8837; r
p=0.9918, RMSEP=39.6042; Correlogram between model predication value and measured value is shown in Figure 14.
Reclaim the foundation of ethanol process aurantiin quantitative model
Application PLS method is set up quantitative model.Modeling parameters: sample NIRS spectrum MSC carries out pre-service, and modeling wave band is 1100～2300nm, number of principal components 9, rejecting abnormalities point.Must reclaim ethanol process aurantiin quantitative model r through cross validation
c=0.9942, RMSEC=70.6075; r
p=0.9891, RMSEP=97.3434; Correlogram between model predication value and measured value is shown in Figure 15.
The foundation of mixing of materials process qualutative model
In application TQ Analyst8.0, collection of illustrative plates similarity technology (Similarity match) is set up qualutative model.Modeling parameters: sample NIRS spectrum smoothly carries out pre-service with first order derivative+Norris, modeling wave band 1000～2500nm.The different sampling spot collection of illustrative plates of mixing of materials process per minute standard deviation stacking diagram sees Figure 16.
According to above-mentioned set up calibration model, the near infrared spectrum data of checking collection sample is imported in model, can fast prediction naringin content.Result shows, the deviation between NIRS predicted value and HPLC measured value is less, and the predictive ability of institute's established model is better, in table 1.
The checking of table 1 Beijing enamel ware blood fat regulating capsule production run near-infrared model
Table 2 Beijing enamel ware blood fat regulating capsule mixing of materials process
From the experimental result in table 1, table 2, set up to contain near infrared online detection method in aurantiin Chinese Traditional Medicine easy, quick, accurate, highly sensitive, precision, good stability.After near infrared forecast model is successfully established, can measure rapidly and accurately the content of unknown sample aurantiin, be particularly useful for the quick quality testing of the each production run of Beijing enamel ware blood fat regulating capsule, thereby improve the stability of Beijing enamel ware blood fat regulating capsule quality, ensure its clinical efficacy.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. contain the near infrared online detection method in aurantiin Chinese Traditional Medicine, it is characterized in that, comprise the steps:
Collection contains the sample in aurantiin Chinese Traditional Medicine and gathers its near infrared spectrum;
Utilize the naringin content in prior art working sample;
The naringin content recording according to the near infrared spectrum of described sample and prior art is set up detection model, described detection model comprises quantitative model and qualutative model, described quantitative model is set up by partial least square method, the spectral range of choosing infrared spectrum is 1100～2300nm, described qualutative model is set up by the collection of illustrative plates similarity technology in TQ Analyst software, and the spectral range of choosing infrared spectrum is 1100～2500nm;
Gather testing sample, utilize described detection model to measure naringin content.
2. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 1, is characterized in that, described prior art is high performance liquid chromatography.
3. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 1, it is characterized in that, the sample containing in aurantiin Chinese Traditional Medicine of described collection is divided into calibration set and checking collection, set up respectively the corresponding relation between the naringin content in calibration set and prior art working sample, recycling checking collection sample is verified detection model, optimizes the detection model containing in aurantiin Chinese Traditional Medicine.
4. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 1, is characterized in that, described in to contain aurantiin Chinese medicine be Beijing enamel ware blood fat regulating capsule.
5. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 4, it is characterized in that, in the production run of described Beijing enamel ware blood fat regulating capsule, sample collection is to collect the extract, concentrate, the ethanol that contain different time points in aurantiin Chinese Traditional Medicine to reclaim liquid and mixture samples.
6. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 5, it is characterized in that, the extract of described different time points refers to that Beijing enamel ware blood fat regulating capsule decocts the extract of each time point in leaching process for the first time, for the second time; The concentrate of different time points refers to the concentrate of each time point in Beijing enamel ware blood fat regulating capsule concentration process; The ethanol of different time points reclaims liquid and refers to that Beijing enamel ware blood fat regulating capsule precipitation solution reclaims the recovery liquid of each time point in ethanol process; The batch mixing of different time points refers to the batch mixing of each time point in Beijing enamel ware blood fat regulating capsule batch mixing process.
7. the near infrared online detection method containing in aurantiin Chinese Traditional Medicine according to claim 6, it is characterized in that, the described parameter that decocts for the first time leaching process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum non-processor, number of principal components 10, rejecting abnormalities point; The described parameter that decocts for the second time leaching process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum smoothly carries out pre-service with first order derivative and Norris, number of principal components 4, rejecting abnormalities point; The parameter of described concentration process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum smoothly carries out pre-service with second derivative and SG, number of principal components 11, rejecting abnormalities point; The parameter of described recovery ethanol process aurantiin quantitative model: wave spectrum scope 1100～2300nm, original spectrum carries out pre-service with polynary scatter correction, number of principal components 10, rejecting abnormalities point; The parameter of described batch mixing process qualutative model: wave spectrum scope 1000～2500nm, original spectrum smoothly carries out pre-service with first order derivative and Norris.
8. according to arbitrary described near infrared online detection method in the aurantiin Chinese Traditional Medicine application in traditional Chinese medicine quality monitoring that contains of claim 1～7.
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| CN110987865A (en) * | 2019-12-13 | 2020-04-10 | 齐鲁工业大学 | Method for detecting fig quality based on near infrared spectrum |
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| CN113484272A (en) * | 2021-07-08 | 2021-10-08 | 云南中烟工业有限责任公司 | Method for rapidly predicting oil content in fresh tobacco leaves by adopting similarity analysis technology based on near infrared spectrum |
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