CN104059808A - Beer and preparation method thereof - Google Patents
Beer and preparation method thereof Download PDFInfo
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- CN104059808A CN104059808A CN201410325379.3A CN201410325379A CN104059808A CN 104059808 A CN104059808 A CN 104059808A CN 201410325379 A CN201410325379 A CN 201410325379A CN 104059808 A CN104059808 A CN 104059808A
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- beer
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Abstract
The invention relates to the field of beer processing and specifically relates to beer and a preparation method thereof. The preparation method of beer comprises the following steps: drying fresh lotus leaves in air and then crushing, extracting nuciferine in the fresh lotus leaves by sequentially adopting a cellulose pre-treating method, a diluted hydrochloric acid leaching method, an ultrasonic wave assisted extraction method and a chloroform extraction method; uniformly mixing and stirring the nuciferine with the beer after the fermentation step in a mass ratio of (0.006-0.010) to 1000, and sterilizing, clarifying by a clarifying agent, freezing, filtering and packaging to obtain the finished product. According to the preparation method of the beer, which is provided by the invention, the prepared beer has better mouthfeel and effect; after the nuciferine is added, the prepared beer can achieve a good effect of lowering lipid and pressure, does not injure spleen and stomach after being drunk much; besides, the processing method is simple and easy to operate.
Description
Technical field
The present invention relates to beer manufacture field, in particular to a kind of beer and preparation method thereof.
Background technology
Beer is that the mankind are the most ancient, is water and the tea beverage that consumption is number three in the world afterwards.Beer imports China in earlier 1900s, belongs to external wine kind.Beer is to be translated into Chinese " beer " according to English Beer, is called " beer ", uses till today.It is main raw material that beer be take Fructus Hordei Germinatus, hops, water, the full carbonated low-alcoholic wine of full beer forming through yeast fermentation effect brew.
Beer all has good auxiliary curative effect to essential hypertension, heart trouble and tuberculosis etc., appropriate drinks beer also has certain curative effect to heart and hyperpietic, but drink, can hurt taste more, increase the weight of moisture in body, therefore invent and a kind ofly not only can play health care lipopenicillinase but also can not drink favor and the concern that the health-care beer of overtaxing one's nerves is more and more subject to people because of excessive.
Summary of the invention
The object of the present invention is to provide a kind of beer and preparation method thereof, to solve the above problems.
The embodiment of the present invention provides a kind of preparation method of beer, comprises the steps:
(A) will after new lotus leaf airing, pulverize, adopt successively cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf;
(B) by the beer after described Nuciferine and step by fermentation, according to mass ratio, be that 0.006-0.010:1000 carries out after mixing and stirring, through sterilizing, finings clarification, freezing, filter and packing after obtain finished product.
The preparation method of a kind of beer that the embodiment of the present invention provides, Nuciferine in new lotus leaf after carrying out fermentation step, beer is added according to certain ratio, the requirement of new lotus leaf and adding proportion is that the certain mouthfeel of the beer in order to guarantee to prepare and the finished product effect prepared are better, add the beer that makes after Nuciferine to prepare and can play the good result of blood fat-reducing blood pressure-decreasing, many drinks yet can not produce and injure taste, without Nuciferine is added together and fermented, only Nuciferine need be added to the beer mix and blend after fermentation, working method is simple, easy to operate.And, in order better to extract the Lipid-lowering activities composition in lotus leaf, by adopting successively four steps of cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method, extract the Nuciferine of the high-content in new lotus leaf, Nuciferine quality and output that this extracting method not only extracts are high, and be added to the hypertension and hyperlipemia effect that can strengthen beer in beer afterwards, can not injure taste yet.
Beer after the present invention's normal fermentation step of process used can be domestic any Yi Jia stdn producer beer, the quality standard of beer is as follows: and State Standard of the People's Republic of China (8 degree, 10 degree, 11 degree, 12 degree top grade pale beers, GB4927-2001).
Preferably, in described step (A), new lotus leaf airing is below 20% to the massfraction of water content, and water content is too high is all unfavorable for follow-up pulverizing and the step of extracting Nuciferine, and the purity of the Nuciferine extracting is also had to impact.
Preferably, in described step (A), the purity of the Nuciferine obtaining is that massfraction reaches more than 98%, and highly purified Nuciferine can make the effect of the beer blood fat-reducing blood pressure-decreasing prepared better.
Preferably, in described step (A), after new lotus leaf airing is pulverized, by the pretreated concrete steps of cellulase, be: adopting consumption is 2.667 * 10
-6-3.000 * 10
-6the cellulase of mo1/ (s.g) is to carry out pre-treatment under the condition of 50-60 ℃ in temperature, enzymolysis time is 2-3h, enzymolysis pH value is controlled between 5.0-6.0, cellulase is under the condition of certain consumption and potential of hydrogen, katalysis is effective, for the better place mat effect of playing of subsequent processing steps, wherein the mo1/ of unit (s.g) refers to the vigor of enzyme, and physical significance is the amount of substance of the enzyme that contains on the substrate of certain quality and volume.
Preferably, in described step (A), the concrete steps of dilute hydrochloric acid lixiviate are by the dilute hydrochloric acid lixiviate 16-18h that is 0.5-0.8% with mass percent concentration with the pretreated new lotus leaf of cellulase.
Preferably, in described step (B), by the beer after described Nuciferine and step by fermentation, according to mass ratio, be that the stirring velocity that 0.006-0.009:1000 carries out mixing and stirring is 500-600rad/min, time is 2-3h, temperature is 25-35 ℃, more even in order to mix when the beer after fermentation mixes with Nuciferine, temperature, time and rotating speed are preferably in the product better effects if to guarantee to prepare in certain scope.
Preferably, in described step (A), the concrete steps of ultrasonic-assisted extraction are for putting into the new lotus leaf after dilute hydrochloric acid lixiviate extracting solvent, and ultrasonic vibration 30-40min, controls temperature at 78-80 ℃, and ultrasonic frequency is controlled at 45-60kHz.Oscillator sends ultrasonic wave to extracting in solvent, ' cavitation effect ' that ultrasonic wave produces in extracting solvent and mechanical effect one side be the cell walls of broken lotus leaf effectively, make effective constituent be unbound state and dissolve in to extract in solvent, can accelerate to extract on the other hand the molecular motion of solvent, making to extract solvent contacts fast with the effective constituent in lotus leaf, fused, mixing, reaches the object that extracts Nuciferine mutually.
Preferably, in described step (B), described finings is one or more in tannic acid, gelatin and egg white.
Preferably, in described step (B), that finings carries out after adding rear standing 24h above is again freezing, filter and packing, in order to make the clarifying effect of beer better, preferably, by more than the standing 24h of the beer of putting into finings, the clarity of product and outward appearance, mouthfeel are all better.
Preferably, in described step (B), Nuciferine is adopted to pasteurization with carrying out sterilizing described in the step of sterilizing after the beer of step by fermentation mixes again, sterilising temp is controlled at 62-65 ℃, the too high meeting of sterilising temp destroys the effective constituent among beer, reduces its effect.
The embodiment of the present invention also provides a kind of a kind of beer that has above-mentioned preparation method to prepare, compare with beer of the prior art, it take Nuciferine is raw material with the beer of step by fermentation, wherein lotus leaf is the blade of nymphaeaceae plant lotus (NELUM BONUCIFERA GAERTN), nymphaeaceae plant, main product in Fujian, the ground such as Jiangxi, Hunan.In < < Compendium of Materia Medica > >, record: effects such as " hair tonic vigour, benefit helps taste, puckery elite, loose blood " " appetite-stimulating indigestion-relieving, hemostasis controlling nocturnal emission with astringent drugs ".In November, 1991, the prison of defending of Ministry of Health of the People's Republic of China was sent out in (1991) No. 45 files, and lotus leaf is put into second batch " being food and medicine " list.Lotus Leafextract Nuciferine is a kind of lipopenicillinase that is applied to, step-down, the aspects such as fat that disappear health-care components the most widely, also be to be subject to west authority's medicine and pharmacology can praise " the holy product of lipopenicillinase ", Nuciferine has outstanding effect of weight reducing, contains multiple effectiveization fat alkaloid, effectively the fat in decomposer, and powerful excreting, has remarkable Antilipemic health effect.Make the beer of preparing possess lipopenicillinase, step-down, the effect of the fat that disappears, and can, because excessive drinking hurts taste, not there is the effect of remarkable health care lipopenicillinase.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment 1
A kind of preparation method of beer is as follows:
(A) will after new lotus leaf airing, pulverize, adopt successively cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf;
(B) the beer 100kg after Nuciferine 0.6g and step is by fermentation carried out after mixing and stirring, through sterilizing, finings clarification, freezing, filter and packing after obtain finished product.
Embodiment 2
(A) by new lotus leaf airing, the massfraction to water content is 18% rear pulverizing, adopts cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf successively, by detecting the massfraction of its purity of Nuciferine, is 98%; Its cellulase pre-treatment is that employing consumption is 2.667 * 10
-6the cellulase of mo1/ (s.g) is to carry out pre-treatment under the condition of 50 ℃ in temperature, and enzymolysis time is 2h, and enzymolysis pH value is controlled at 5.0, and dilute hydrochloric acid lixiviate is the dilute hydrochloric acid lixiviate 16h that is 0.5% with mass percent concentration;
(B) Nuciferine 0.9g and beer 100kg after normal fermentation step are carried out after mixing and stirring, through sterilizing, finings clarification, freezing, filter and packing after obtain finished product.
Embodiment 3
(A) by new lotus leaf airing, the massfraction to water content is 17% rear pulverizing, adopts cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf successively, by detecting the massfraction of its purity of Nuciferine, is 99%; Its cellulase pre-treatment is that employing consumption is 3.000 * 10
-6the cellulase of mo1/ (s.g) is to carry out pre-treatment under the condition of 60 ℃ in temperature, and enzymolysis time is 2h, and enzymolysis pH value is controlled at 6.0, and dilute hydrochloric acid lixiviate is the dilute hydrochloric acid lixiviate 18h that is 0.8% with mass percent concentration; Wherein the concrete steps of ultrasonic-assisted extraction are for putting into the new lotus leaf after dilute hydrochloric acid lixiviate extracting solvent, and ultrasonic vibration 30min, controls temperature at 78 ℃, and ultrasonic frequency is controlled at 45kHz.
(B) Nuciferine 1g and the beer 100kg after normal fermentation step are carried out to mixing and stirring, stirring velocity is 600rad/min, and churning time is 3h, and whipping temp is 35 ℃, wherein the consumption of Nuciferine can also select to add 0.7g, 0.8g and 0.85g;
(C) fully stir later through temperature be bus sterilizing under 65 ℃ of conditions, gelatin and egg white mix clarify 26h after, after freezing, filtration residue and packing, obtain finished product.
Embodiment 4
(A) by new lotus leaf airing, the massfraction to water content is 20% rear pulverizing, adopts cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf successively, by detecting the massfraction of its purity of Nuciferine, is 98%; Its cellulase pre-treatment is that employing consumption is 2.900 * 10
-6the cellulase of mo1/ (s.g) is to carry out pre-treatment under the condition of 55 ℃ in temperature, and enzymolysis time is 2.5h, and enzymolysis pH value is controlled at 5.5, and dilute hydrochloric acid lixiviate is the dilute hydrochloric acid lixiviate 17h that is 0.5% with mass percent concentration; Wherein the concrete steps of ultrasonic-assisted extraction are for putting into the new lotus leaf after dilute hydrochloric acid lixiviate extracting solvent, and ultrasonic vibration 40min, controls temperature at 80 ℃, and ultrasonic frequency is controlled at 60kHz.
(B) Nuciferine 0.9g and the beer 100kg after normal fermentation step are carried out to mixing and stirring, stirring velocity is 500rad/min, and churning time is 2h, and whipping temp is 25 ℃,
(C) fully stir later through after temperature is bus sterilizing under 62 ℃ of conditions, tannic acid clarification 24h, obtain finished product after freezing, filtration residue and packing.
Experimental example 1
The beer that embodiment of the present invention 1-4 is prepared is respectively prepared bottled 900 bottles of 500ml specification, and bottled 900 bottles of the Snowflake Beer 500ml specification of selling on the market, 900 bottled beers that embodiment 1 prepares are first group, 900 bottled beers that embodiment 2 prepares are second group, 900 bottled beers that embodiment 3 prepares are the 3rd group, 900 bottled beers that embodiment 4 prepares are the 4th group, 900 bottles of the Snowflake Beer sold are on the market the 5th group, the number of degrees of beer are 8 degree, randomly draw 50 people, 10 artificial one group, first 10 people drink 900 bottled beers of first group, drinking method is: every day, supper was drunk one bottle, drink 3 months, second 10 people drinks 900 bottled beers of second group, the like, each is like this per capita, after three months, the people after finishing off is carried out to susceptibility investigation, investigation result is as follows.
Table 1 is tried out result
As seen from Table 1, drunk the people of beer of the present invention, do not feel that taste are uncomfortable but also blood fat and blood pressure is had some improvement, the beer that the present invention prepares by preparation method of the present invention after adding Nuciferine is described, can make people's long-term drinking of drinking also there will not be the uncomfortable situation of taste, belong to a kind of health care beer, can not injure taste, and the words of drinking the common Snowflake Beer of selling on the market, in the short period of time of 3 months, can not improve significantly to blood fat and blood pressure, may need drinking of longer time to have a little effect, but also can feel that taste are uncomfortable.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. a preparation method for beer, is characterized in that, comprises the steps:
(A) will after new lotus leaf airing, pulverize, adopt successively cellulase pre-treatment, dilute hydrochloric acid lixiviate, ultrasonic-assisted extraction, chloroform extraction method to extract the Nuciferine in new lotus leaf;
(B) by the beer after described Nuciferine and step by fermentation, according to mass ratio, be that 0.006-0.010:1000 carries out after mixing and stirring, through sterilizing, finings clarification, freezing, filter and packing after obtain finished product.
2. the preparation method of a kind of beer according to claim 1, is characterized in that, in described step (A), new lotus leaf airing to the massfraction of water content is below 20%.
3. the preparation method of a kind of beer according to claim 1, is characterized in that, in described step (A), the massfraction of the purity of the Nuciferine obtaining reaches more than 98%.
4. the preparation method of a kind of beer according to claim 1, is characterized in that, in described step (A), is: adopting consumption is 2.667 * 10 after new lotus leaf airing is pulverized by the pretreated concrete steps of cellulase
-6-3.000 * 10
-6the cellulase of mo1/ (s.g) is to carry out pre-treatment under the condition of 50-60 ℃ in temperature, and enzymolysis time is 2-3h, and enzymolysis pH value is controlled between 5.0-6.0.
5. the preparation method of a kind of beer according to claim 1, it is characterized in that, in described step (A), the concrete steps of dilute hydrochloric acid lixiviate are by the dilute hydrochloric acid lixiviate 16-18h that is 0.5-0.8% with mass percent concentration with the pretreated new lotus leaf of cellulase.
6. the preparation method of a kind of beer according to claim 1, it is characterized in that, in described step (A), the concrete steps of ultrasonic-assisted extraction are for putting into the new lotus leaf after dilute hydrochloric acid lixiviate extracting solvent, ultrasonic vibration 30-40min, control temperature at 78-80 ℃, ultrasonic frequency is controlled at 45-60kHz.
7. the preparation method of a kind of beer according to claim 1, it is characterized in that, in described step (B), by the beer after described Nuciferine and step by fermentation, according to mass ratio, be that the stirring velocity that 0.006-0.009:1000 carries out mixing and stirring is 500-600rad/min, time is 2-3h, and temperature is 25-35 ℃.
8. the preparation method of a kind of beer according to claim 1, is characterized in that, in described step (B), that finings carries out after adding rear standing 24h above is again freezing, filter and packing.
9. the preparation method of a kind of beer according to claim 1, it is characterized in that, in described step (B), Nuciferine is adopted to pasteurization with carrying out sterilizing described in the step of sterilizing after the beer of step by fermentation mixes again, sterilising temp is controlled at 62-65 ℃.
10. a beer of preparing according to the preparation method of the beer described in claim 1-9 any one.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410325379.3A CN104059808B (en) | 2014-07-09 | 2014-07-09 | A kind of beer and preparation method thereof |
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| CN201410325379.3A CN104059808B (en) | 2014-07-09 | 2014-07-09 | A kind of beer and preparation method thereof |
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| CN104059808A true CN104059808A (en) | 2014-09-24 |
| CN104059808B CN104059808B (en) | 2016-03-09 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108450941A (en) * | 2018-03-13 | 2018-08-28 | 武春风 | A kind of dietary composition adjusting lipid metaboli |
| CN109349624A (en) * | 2017-11-06 | 2019-02-19 | 江苏武惠堂食品有限公司 | A kind of preparation method of Folium Nelumbinis essenc |
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| CN1297030A (en) * | 2000-10-31 | 2001-05-30 | 姚力克 | Health-care beer |
| CN101139320A (en) * | 2007-09-01 | 2008-03-12 | 福州大学 | Method for separating nuciferine and lotus leaf flavone from lotus leaf |
| CN102220211A (en) * | 2011-06-20 | 2011-10-19 | 庞霞 | Method for making special yellow wine containing lotus leaf flavonoids |
| CN103087888A (en) * | 2013-03-05 | 2013-05-08 | 宜宾龙门酒业有限公司 | Lotus leaf wine and preparation method thereof |
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2014
- 2014-07-09 CN CN201410325379.3A patent/CN104059808B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1297030A (en) * | 2000-10-31 | 2001-05-30 | 姚力克 | Health-care beer |
| CN101139320A (en) * | 2007-09-01 | 2008-03-12 | 福州大学 | Method for separating nuciferine and lotus leaf flavone from lotus leaf |
| CN102220211A (en) * | 2011-06-20 | 2011-10-19 | 庞霞 | Method for making special yellow wine containing lotus leaf flavonoids |
| CN103087888A (en) * | 2013-03-05 | 2013-05-08 | 宜宾龙门酒业有限公司 | Lotus leaf wine and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| 李凤发: "今夏啤酒新品多", 《金融时报》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109349624A (en) * | 2017-11-06 | 2019-02-19 | 江苏武惠堂食品有限公司 | A kind of preparation method of Folium Nelumbinis essenc |
| CN108450941A (en) * | 2018-03-13 | 2018-08-28 | 武春风 | A kind of dietary composition adjusting lipid metaboli |
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| CN104059808B (en) | 2016-03-09 |
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