CN104056857A - Electrically-assistant microbial remediation method of oil polluted soil - Google Patents
Electrically-assistant microbial remediation method of oil polluted soil Download PDFInfo
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- CN104056857A CN104056857A CN201310112380.3A CN201310112380A CN104056857A CN 104056857 A CN104056857 A CN 104056857A CN 201310112380 A CN201310112380 A CN 201310112380A CN 104056857 A CN104056857 A CN 104056857A
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Abstract
The invention discloses an electrically-assistant microbial remediation method of oil polluted soil, which comprises the following steps: (1) adding a surfactant solution into oil polluted soil; (2) adding N, P nutrition solutions into the soil treated in step (1); (3) adding 6-8% of petroleum degrading bacteria into the soil treated in step (2), well mixing; (4) adding 2% of an organic additive (W/W) into the soil treated in step (3); (5) weaving resistance wires on steel wire nets in a snake-shaped reciprocating manner to prepare resistance wire heating plates, connecting the resistance wire heating plates with a temperature controller in parallel, and externally connecting the resistance wire heating plates with a power plug; (6) putting the oil polluted soil treated in step (4) into a reaction tank, putting the resistance wire heating plate at the bottom end and the top end of the oil polluted soil, inserting a probe of the temperature controller into the bottom of the oil polluted soil, and turning on the power. The method of the invention can realize remediation of soil with high oil content, and has the advantages of short remediation time, high polluted oil removal rate, and suitability for low temperature environment. The figure is a flow chart of the electrically-assistant microbial remediation method of oil polluted soil.
Description
Technical field
The present invention relates to soil contaminated by crude oil restorative procedure, relate in particular to the electronic auxiliary repairing method of microorganism of soil contaminated by crude oil, belong to soil contaminated by crude oil biological restoration field.
Technical background
In today of economic develop rapidly, oil has become the core of the contemporary economy of energy.But along with the growing demand of the world to oil and goods thereof, in oil exploitation, processing and transportation, because all causing petroleum pollution, the burning of crude oil leakage, crude oil processing factory, equipment pollution and oil etc. flows into environment, cause serious environmental pollution, environment has been damaged.Therefore, the problem of oil pollution can not be ignored, and has become countries in the world common concern and environmental problem urgently to be resolved hurrily.
The method of processing sump oil mainly contains the methods such as physics landfill, burning and biological restoration, wherein bioremediation technology have safety, efficient, economical, not spoiled soil environment, pollutant oxidation completely, do not have secondary pollution, can in-situ treatment relative with the operation advantage such as simple are considered to have wide application prospect and receive much concern, be also the main research and development direction of processing at present oil pollution problem.
But in soil contaminated by crude oil biological restoration process, except the selection of efficient microbial strain, also have the factor that much affects repairing effect, comprise the factor such as nutrient content, ventilation degree in soil contaminated by crude oil.Poor containing the soil aeration that sump oil amount is higher, cause microbial reproduction slow, remediation efficiency is low, and the time is longer, and this is also the main cause of restriction soil contaminated by crude oil bioremediation technology application.Therefore, setting up soil contaminated by crude oil bioremediation technology is fast the task of top priority that solves soil polluted by oil.
Summary of the invention
Technical problem solved by the invention is to overcome the problems such as general petroleum-contaminated soil biological renovation method is poor to the soil contaminated by crude oil repairing effect of floorboard with high oil content (2%-10%), the rate of deoiling is low, the time is long, provide a kind of new soil contaminated by crude oil electronic auxiliary repairing method of microorganism, the method can be repaired the greasy dirt loam earth of floorboard with high oil content, and has short, the advantage such as sump oil clearance high and low temperature environment still can be applied repair time.
Technical problem to be solved by this invention is achieved through the following technical solutions:
The electronic auxiliary repairing method of microorganism of a kind of soil contaminated by crude oil, comprises the following steps: (1) is to the surfactant solution adding in soil contaminated by crude oil; (2) in step (1) soil contaminated by crude oil after treatment, add N, P nutritive solution; (3) pollute degradation flora to the high efficient oil that adds 6-8% in step (2) soil contaminated by crude oil after treatment, mix; (4) in step (3) soil contaminated by crude oil after treatment, add 2% organic additive (W/W); (5) resistance wire is woven on steel wire by snakelike coming and going, makes Resistant heating plate, temperature controller in parallel simultaneously, external power supply plug; (6) petroleum-contaminated soil of step (4) being processed is put into reaction tank, and Resistant heating plate is placed on to bottom and the top of soil contaminated by crude oil, and thermostat probe is inserted to soil contaminated by crude oil bottom, switches on power.
Wherein, the surfactant of the interpolation described in step (1) is Tween80, and it adds concentration is 0.06% of soil contaminated by crude oil quality.
The N of the interpolation described in step (2), P nutrient are NH
4nO
3and K
2hPO
4, the mass fraction using the quality of sump oil in petroleum-contaminated soil as C, adding proportion is C:NH
4nO
3: K
2hPO
4=100: 6: 1.
The degradation flora adding in step (3) is by 6 kinds of Fungal community compositions: sub-sticky group sickle-like bacteria (Fusarium subglutinans), a Pseudoallescheria boydii (pseudallescheria boydii), cot penicillium bacterium (Eupenicillium crustaceum), powder Isaria bacterium (lsaria farinosa), aspergillus terreus (Aspergillus terreus), wart stalk Aspergillus (Aspergillus protuberus).In order to reach better effect, 6 kinds of fungies are first cultivated respectively mixing composition flora again and add soil contaminated by crude oil, require single bacterial classification concentration to reach 10
6individual/more than mL.
Organic additive in step (4) is the corncob of pulverizing, and diameter is less than 1.5cm.
The resistance wire spacing 4-5cm of the making Resistant heating plate in step (5).
Two Resistant heating distances between plates in step (6) are 20-25cm, and when circuit is connected, electric current is 0.6 ± 0.3A.
The electronic auxiliary repairing method of microorganism of soil contaminated by crude oil of the present invention mainly has advantages of following several respects:
1, can process the soil contaminated by crude oil of floorboard with high oil content: adopt restorative procedure of the present invention, by adding the corncob of surfactant Tween80 and pulverizing, can increase the aeration of soil contaminated by crude oil, the soil contaminated by crude oil for high oil content below 10% can effectively be repaired.
2, it is fast that sump oil clearance is high, soil contaminated by crude oil is repaired speed: adopt restorative procedure of the present invention, in one month, the soil contaminated by crude oil rate of deoiling that contains 10% crude oil reaches more than 75%, and the soil contaminated by crude oil of oil content 2%-5% deoils rate all more than 85%, can reach 90% at most.
3, low temperature environment still can be applied: adopt restorative procedure of the present invention, utilize Resistant heating plate still can use 10 DEG C of left and right, and the reparation speed of soil contaminated by crude oil is unaffected.
In a word, the present invention has and can process that floorboard with high oil content soil contaminated by crude oil, sump oil clearance are high, soil contaminated by crude oil is repaired the advantages such as speed is fast, low temperature environment still can be applied.
Brief description of the drawings
The flow chart of the electronic auxiliary repairing method of microorganism of Fig. 1 soil contaminated by crude oil of the present invention
The temperature controller of the electronic auxiliary repairing method of microorganism of Fig. 2 soil contaminated by crude oil of the present invention
The resistive heater of the electronic auxiliary repairing method of microorganism of Fig. 3 soil contaminated by crude oil of the present invention
Resistant heating plate and the temperature regulating device of the electronic auxiliary repairing method of microorganism of Fig. 4 soil contaminated by crude oil of the present invention
The installation demonstration graph of the electronic auxiliary repairing method of microorganism of Fig. 5 soil contaminated by crude oil of the present invention
The exploded view of the electronic auxiliary repairing method of microorganism of Fig. 6 soil contaminated by crude oil of the present invention
Detailed description of the invention
Line face further describes the present invention in conjunction with specific embodiments, and advantage and disadvantage of the present invention will be more clear along with description, but these embodiment are only exemplary, scope of the present invention are not formed to any restriction.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these amendments and replacement all fall within the scope of protection of the present invention.
Embodiment 1
(1) pretreatment of soil contaminated by crude oil
To the surfactant Tween80 solution that adds 500 times of 0.06% dilutions in soil contaminated by crude oil by the mass ratio of soil; Mass fraction using the content of soil contaminated by crude oil Petroleum Hydrocarbon as C again, with NH
4nO
3and K
2hPO
4as the mass fraction of N and P, according to C: N: P=100: the ratio of 6: 1 is added and the isopyknic NH of surfactant in soil contaminated by crude oil
4nO
3and K
2hPO
4solution.
(2) interpolation of flora
First cultivate separately 6 kinds of fungies in flora: sub-group sickle-like bacteria (Fusarium subglutinans), Pseudoallescheria boydii (pseudallescheria boydii), cot penicillium bacterium (Eupenicillium crustaceum), powder Isaria bacterium (lsaria farinosa), aspergillus terreus (Aspergillus terreus), the wart stalk Aspergillus (Aspergillus protuberus) of gluing, treat that bacterial classification concentration reaches 10
6individual/when mL, 6 bacterial classifications to be mixed, by the 6-8% interpolation flora of soil contaminated by crude oil quality, mixing is mixed thoroughly.
(3) adding of organic additive
By soil contaminated by crude oil quality 2% to the corncob that adds pulverizing in soil contaminated by crude oil, agitation as appropriate is mixed.
(4) making of heating and temperature controlling device
As shown in Figure 4, by reserving the length of 20-25cm in the middle of resistance wire, be covered with steel wire respectively on two steel wires with snakelike round braiding, spacing is 4-5cm, and wherein connect with temperature controller in one end, an attaching plug of one end link of the other end and controller.
(5) installation and soil contaminated by crude oil reparation
Resistant heating plate a slice of making is placed on and repairs bottom, pond, in covering, add the soil contaminated by crude oil of the preparations such as the flora of high about 20cm, cover again upper another sheet resistance silk heating plate, top covers the soil contaminated by crude oil of thick about 2cm, the bottom of the probe of thermostat being inserted to reaction tank, switches on power.
Comparative experimental example 1
One, test method: test method is divided into test group and control group, in soil, sump oil content is 5%.
Test group: point 15-16 DEG C and 20-22 DEG C of two environmental temperature experiments, for adopting the electronic auxiliary repairing method of microorganism of soil contaminated by crude oil of embodiment 1, temperature controller control temperature setting is set to 20 DEG C and 30 DEG C, and the test period is one month; Wherein resistance wire total length is 5.2 meters, and resistance is 70 Ω/rice, and voltage is 220V;
Control group: point 15-16 DEG C, 20-22 DEG C and 30-33 DEG C of three environmental temperature experiments, test method is the soil contaminated by crude oil repairing method of microorganism that does not use Resistant heating plate and thermostat in test group, the test period is one month.
Two, result of the test: comparative test result is in table 1
The electronic auxiliary microorganism of table 1 and repairing method of microorganism comparison
From comparative test result, no matter at low temperature environment or in hot environment, the petroleum degradation rate of test group is apparently higher than the control group petroleum degradation rate of same environment temperature and repairing environment temperature.
Claims (9)
1. the electronic auxiliary repairing method of microorganism of soil contaminated by crude oil, comprises the following steps: (1) is to the surfactant solution adding in soil contaminated by crude oil; (2) in step (1) soil contaminated by crude oil after treatment, add N, P nutritive solution; (3) pollute degradation flora to the high efficient oil that adds 6-8% in step (2) soil contaminated by crude oil after treatment, mix; (4) in step (3) soil contaminated by crude oil after treatment, add 2% organic additive (W/W); (5) resistance wire is woven on steel wire by snakelike coming and going, makes Resistant heating plate, temperature controller in parallel simultaneously, external power supply plug; (6) petroleum-contaminated soil of step (4) being processed is put into reaction tank, and Resistant heating plate is placed on to bottom and the top of soil contaminated by crude oil, and thermostat probe is inserted to soil contaminated by crude oil bottom, switches on power.
2. require the restorative procedure described in 1 according to patent, it is characterized in that: the surfactant of the interpolation described in step (1) is Tween80, it adds concentration is 0.06% of soil contaminated by crude oil quality.
3. require the restorative procedure described in 1 according to patent, it is characterized in that: the N of the interpolation described in step (2), P nutrient are NH
4nO
3and K
2hPO
4, the mass fraction using the quality of sump oil in petroleum-contaminated soil as C, according to C: NH
4nO
3: K
2hPO
4=100: within 6: 1, ratio is added.
4. require the restorative procedure described in 1 according to patent, it is characterized in that: the degradation flora adding in step (3) is by 6 kinds of Fungal community compositions: sub-sticky group sickle-like bacteria (Fusarium subglutinans), a Pseudoallescheria boydii (pseudallescheriaboydii), cot penicillium bacterium (Eupenicillium crustaceum), powder Isaria bacterium (lsaria farinosa), aspergillus terreus (Aspergillus terreus), wart stalk Aspergillus (Aspergillus protuberus).
5. require the restorative procedure described in 1 according to patent, it is characterized in that: the degradation flora adding in step (3) is before adding, and 6 kinds of fungies are cultivated respectively, and fungi concentration reaches 10
6when individual/mL is above, mixes and use.
6. require the restorative procedure described in 1 according to patent, it is characterized in that: the organic additive in step (4) is the corncob of pulverizing, and diameter is less than 1.5cm.
7. require the restorative procedure described in 1 according to patent, it is characterized in that: the resistance wire spacing in step (5) is 4-5cm.
8. require the restorative procedure described in 1 according to patent, it is characterized in that: two resistance heated distances between plates in step (6) are 20-25cm.
9. require the restorative procedure described in 1 according to patent, it is characterized in that: when the circuit in step (6) is connected, electric current is 0.6 ± 0.3A.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112380.3A CN104056857B (en) | 2013-03-22 | 2013-03-22 | A kind of electronic auxiliary repairing method of microorganism of soil contaminated by crude oil |
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|---|---|---|---|
| CN201310112380.3A CN104056857B (en) | 2013-03-22 | 2013-03-22 | A kind of electronic auxiliary repairing method of microorganism of soil contaminated by crude oil |
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| CN104056857B CN104056857B (en) | 2017-10-27 |
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Cited By (8)
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| CN104492807A (en) * | 2014-12-15 | 2015-04-08 | 中国石油天然气股份有限公司 | Microbial remediation equipment for treating petroleum-contaminated soil and remediation method of microbial remediation equipment |
| CN104588405A (en) * | 2015-01-19 | 2015-05-06 | 中国科学院沈阳应用生态研究所 | Microorganism-electric power two-segment repairing method for petroleum contaminated soils |
| CN105013815A (en) * | 2015-07-28 | 2015-11-04 | 湖南大学 | Biological remediation method for polycyclic aromatic hydrocarbon and heavy metal compound contaminated soil |
| CN107671116A (en) * | 2017-11-16 | 2018-02-09 | 北京高能时代环境技术股份有限公司 | A kind of improved organic material contaminated soil or underground water In-situ resistance heating hot repair complex system and hot restorative procedure |
| CN108676732A (en) * | 2018-07-20 | 2018-10-19 | 四川农业大学 | Vacation Escherichia WNF15 one plant oval and its application |
| CN109226251A (en) * | 2018-10-24 | 2019-01-18 | 邵亦飞 | The ex-situ repair system and method for oil-polluted soils |
| CN110614270A (en) * | 2019-08-26 | 2019-12-27 | 北京石油化工学院 | Experimental device and method for repairing organic contaminated soil by coupling resistance heating with microorganisms |
| CN110842020A (en) * | 2019-12-02 | 2020-02-28 | 上海康恒环境修复有限公司 | Method for repairing petroleum-polluted soil by heat-strengthening-assisted in-situ microorganisms |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110614270A (en) * | 2019-08-26 | 2019-12-27 | 北京石油化工学院 | Experimental device and method for repairing organic contaminated soil by coupling resistance heating with microorganisms |
| CN110842020A (en) * | 2019-12-02 | 2020-02-28 | 上海康恒环境修复有限公司 | Method for repairing petroleum-polluted soil by heat-strengthening-assisted in-situ microorganisms |
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