CN101091957A

CN101091957A - Method for restoring petroleum polluted soil by microbe

Info

Publication number: CN101091957A
Application number: CN 200710055897
Authority: CN
Inventors: 冯江; 王小雨; 王升忠
Original assignee: Northeast Normal University
Current assignee: Northeast Normal University
Priority date: 2007-07-17
Filing date: 2007-07-17
Publication date: 2007-12-26
Anticipated expiration: 2027-07-17
Also published as: CN101091957B

Abstract

The invention relates to a method to restore soil polluted by petroleum by adopting microorganism and the surface active agent produced by the microorganism. The invention restores the soil by adopting two strains of high efficiency degradation fungus Rhodococcus erythropolis and Pseudomonas aeruginosa and the surface active agent from Rhodococcus erythropolis. It can sharply improve degradation efficiency of soil petroleum hydrocarbon. The sample soil contains petroleum hydrocarbon 6500-8500 mg/kg, after degradation 25-60 days' degradation, the total petroleum hydrocarbon content is 1600-600mg/kg, and degradation efficiency is 80-93%.

Description

The repairing method of microorganism of oil-polluted soils

Technical field

The invention belongs to restorative procedure, be specifically related to utilize the biosurfactant reparation of microorganism and generation thereof to be subjected to the method for petroleum hydrocarbon contaminated soil northern wetland oilfield exploitation district to contaminated soil.

Background technology

Along with developing rapidly of China Petroleum, set up the oil field of different scales in many wetland zones, petroleum-polluted wetland area is also more and more.For example, Daqing oil field and Jilin Oil Field are positioned at the wetland district of two sides, downstream, Nenjiang, song-Nen plain innerland, and the Liaohe Oil Field is positioned at Liaohe delta wetland district, and Jilin Oil Field part mining area has entered south, Mo Moge nature reserve area and looked into the nature reserve area, dry lake.The foundation in oil field is very remarkable to the infringement of wetlands ecosystems, the deterioration that pollution sources such as the discarded slurry that oil exploration and a plurality of links such as drilling well, underground work and oil recovery in the development process are produced, ground crude oil, greasy filth and transportation and storage process all can bring well site and near soil soil property thereof has destroyed the ecological environment of wetlands ecosystems.Soil pollution petroleum hydrocarbon problem shows especially day by day, and Wetland ecological environment and biology are constituted a serious threat.

The common method of the petroleum hydrocarbon contaminated reparation of soil comprises: physics, chemistry and biological method.Compare with chemical method with physics, biological restoration particularly microorganism is repaired and to be had characteristics such as cost is low, environmental friendliness, is one of developing direction of the tool potentiality of the petroleum hydrocarbon contaminated reparation of soil.

Exist the microorganism of a large amount of dependence organic matter lives in the soil,, have the organic huge ability of oxidation Decomposition as bacterium, fungi, actinomyces etc.After being subjected to oil pollution, some microorganisms produce the enzyme system of decomposing pollutant under the inducing of pollutant, contaminant degradation can be transformed.

Summary of the invention

The synergy reparation that the present invention utilizes the wetland oilfield exploitation to distinguish from the biosurfactant (sugar esters compounds) of 2 high-efficiency degradation bacterium-rhodococcus erythropolis (Rodococcus erythropolis) that screening obtains and verdigris first monad (Pseudomonas aeruginosa) and rhodococcus erythropolis (more than be known bacterial classification) generation is subjected to petroleum hydrocarbon contaminated soil, and the present invention can improve the degradation efficiency of soil petroleum hydrocarbon significantly.

The present invention is achieved by following technical proposals:

(1) will gather greasy dirt soil near the oil well, air-dry 20 ~ 30 days, remove volatile hydrocarbon and moisture content.

(2) rhodococcus erythropolis and verdigris first monad are cooperated Mixed Microbes in 1: 1 ratio, it is in 6500 ~ 8500 mg/kg soil that bacterium liquid is inserted the total petroleum hydrocarbon initial content, and connecing the bacterium amount is 1.0 * 10 ⁸~ 1.5 * 10 ⁸Individual/g soil, add 1000 ~ 2000mg/kg biosurfactant again, add nutritive salt (nitrogenous source and phosphorus source), add H simultaneously ₂O ₂5 ~ 10mg.

(3) add 5 ~ 10% wood chips and mix, and the adjusting moisture content is 15 ~ 25%.With the vessel surface covered with plastic film, prick the hole ventilation, degraded 50 ~ 60 days down at 25 ~ 30 ℃.

Implementation result

Tried that the total petroleum hydrocarbon initial content is 6500 ~ 8500mg/kg in the soil, after 25 ~ 60 days degraded, total petroleum hydrocarbons content is 1600 ~ 600mg/kg in the soil, and degradation efficiency is 80 ~ 93%.

The specific embodiment

Embodiment 1

(1) will gather greasy dirt soil near the oil well, air-dry 30 days, remove volatile hydrocarbon and moisture content.

(2) rhodococcus erythropolis and verdigris first monad are cooperated Mixed Microbes in 1: 1 ratio, it is in the 8037mg/kg soil that bacterium liquid is inserted the total petroleum hydrocarbon initial content, and connecing the bacterium amount is 1.1 * 10 ⁸Individual/g soil, add the 1000mg/kg biosurfactant again, add KNO ₃And K ₂HPO ₄As nutritive salt, add H simultaneously ₂O ₂5mg.

(3) add 6% wood chip and mix, and the adjusting moisture content is 25%.With the vessel surface covered with plastic film, prick the hole ventilation, total petroleum hydrocarbons content is 597mg/kg in the soil after 30 ℃ were degraded 58 days down, degradation efficiency is 93%.

Embodiment 2

(1) will gather greasy dirt soil near the oil well, air-dry 25 days, remove volatile hydrocarbon and moisture content.

(2) rhodococcus erythropolis liquid and verdigris first monad cooperate Mixed Microbes in 1: 1 ratio, and inserting the total petroleum hydrocarbon initial content is in the 8460mg/kg soil, and connecing the bacterium amount is 1.5 * 10 ⁸Individual/g soil, add the 2000mg/kg biosurfactant, add KNO ₃And K ₂HPO ₄As nutritive salt, add H simultaneously ₂O ₂7mg.

(3) add 8% wood chip and mix, and the adjusting moisture content is 25%.With the vessel surface covered with plastic film, prick the hole ventilation, total petroleum hydrocarbons content is 804mg/kg in the soil after 25 ℃ were degraded 55 days down, degradation efficiency is 90.5%.

Embodiment 3

(1) will gather greasy dirt soil near the oil well, air-dry 20 days, remove volatile hydrocarbon and moisture content.

(2) rhodococcus erythropolis liquid being inserted the total petroleum hydrocarbon initial content is in the 6500mg/kg soil, and connecing the bacterium amount is 1.0 * 10 ⁸Individual/g soil, do not add biosurfactant, add KNO ₃And K ₂HPO ₄As nutritive salt, add H simultaneously ₂O ₂10mg.

(3) add 10% wood chip and mix, and the adjusting moisture content is 25%.With the vessel surface covered with plastic film, prick the hole ventilation, total petroleum hydrocarbons content is 1235mg/kg in the soil after 20 ℃ were degraded 50 days down, degradation efficiency is 81%.

Claims

1, the repairing method of microorganism of oil-polluted soils is characterized in that, concrete steps are as follows:

(1) will gather greasy dirt soil near the oil well, air-dry 20 ~ 30 days, remove volatile hydrocarbon and moisture content;

(2) rhodococcus erythropolis and verdigris first monad are cooperated Mixed Microbes in 1: 1 ratio, it is in 6500 ~ 8500mg/kg soil that bacterium liquid is inserted the total petroleum hydrocarbon initial content, and connecing the bacterium amount is 1.0 * 10 ⁸~ 1.5 * 10 ⁸Individual/g soil, add 1000 ~ 2000mg/kg biosurfactant again, add nutritive salt nitrogenous source or phosphorus source, add H simultaneously ₂O ₂5 ~ 10mg;

(3) add 5 ~ 10% wood chips and mix, and the adjusting moisture content is 15 ~ 25%; With the vessel surface covered with plastic film, prick the hole ventilation, degraded 50 ~ 60 days down at 25 ~ 30 ℃.

2, by the repairing method of microorganism of the described oil-polluted soils of claim 1, it is characterized in that the nutritive salt of described adding is nitrogenous source.

3, by the repairing method of microorganism of the described oil-polluted soils of claim 1, it is characterized in that the nutritive salt of described adding is the phosphorus source.

4, by the repairing method of microorganism of claim 2,3 described oil-polluted soils, it is characterized in that:

(1) will gather greasy dirt soil near the oil well, air-dry 25 days, remove volatile hydrocarbon and moisture content;

(2) rhodococcus erythropolis liquid and verdigris first monad cooperate Mixed Microbes in 1: 1 ratio, and inserting the total petroleum hydrocarbon initial content is in the 8460mg/kg soil, and connecing the bacterium amount is 1.5 * 10 ⁸Individual/g soil, add the 2000mg/kg biosurfactant, add KNO ₃And K ₂HPO ₄As nutritive salt nitrogenous source or phosphorus source, add H simultaneously ₂O ₂7mg;

(3) add 8% wood chip and mix, and to regulate moisture content be 25%,, prick the hole ventilation the vessel surface covered with plastic film, 25 ℃ down degraded after 55 days in the soil total petroleum hydrocarbons content be 804mg/kg, degradation efficiency is 90.5%.