CN104055812B - A kind of composition and preparation method and purposes with antidepressant effect - Google Patents
A kind of composition and preparation method and purposes with antidepressant effect Download PDFInfo
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Abstract
The present invention relates to a kind of antidepressant composition, said composition is made up of eleutheroside and rhodioside by weight for 1~10: 1~10.The invention also discloses the preparation method of said composition.The present composition can apply to the preparation of antidepressant.
Description
This application claims Patent Office of the People's Republic of China, Application No. 201410102755.2, invention were submitted on 03 13rd, 2014
It is entitled：The priority of the Chinese patent application of " a kind of composition and preparation method and purposes with antidepressant effect ",
Entire contents are hereby incorporated by reference in the application.
Technical field
The invention belongs to the field of Chinese medicines, more particularly to a kind of composition and its method and purposes with antidepressant effect
Background technology
Depression be a class with the low depressed mood sexual dysfunction for principal character of mental state, be mainly shown as that lasting mood is low
Fall, retardation of thinking, bulesis decline etc..There is this disease high ill, high recurrence, height to disable, high medical treatment cost the characteristics of, seriously
Endanger human physical and mental health.The incidence of disease of depression is very high, but is not still fully aware of to its pathogenic factor now.Typically
Think, the pathogenesis of depression and the monoamine neurotransmitter of intracerebral are extremely relevant, including serotonin, noradrenaline
Element, adrenaline and dopamine etc..Traditional doctor trained in Western medicine antidepressants are MAOI, and conventional includes benzene second
Hydrazine, Moclobemide, there is tricyclic antidepressant and tetracyclic antidepressants in addition.With the development of science and technology, people are to depression
Going deep into for disease and antidepressant research, then develops serotonin reuptake inhibitor Prozac etc..But this kind of anti-suppression
The drawbacks of strongly fragrant medicine has certain, topmost performance is that side effect is strong, easily causes cardiovascular system injury and hepatic injury
Etc..Comparatively speaking, Chinese medicine plays powerful advantage in anti depressant therapy, such as contains Multiple components and targets a variety of
Albumen target spot, therefore medicine of the exploitation based on Chinese traditional medicine composition has reduction side effect, the advantages of reducing sequelae, instant effect.This hair
The bright deficiency that compensate for foregoing pharmaceutical or treatment method, with good effect, preparation method is simple, the advantages of cost is relatively low.
The content of the invention
It is an object of the present invention to provide a kind of composition with antidepressant effect, it is characterised in that the combination
Thing is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 1~10: 1~10；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 1: 10；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 10: 1；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 1: 9；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 9: 1；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 1: 2；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 1: 3；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 2: 3；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 3: 2；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 3: 1；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 5: 8；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 8: 3；
It is preferred that, said composition is consisted of the following composition：The weight ratio of eleutheroside and rhodioside is 7: 4.
It is also another object of the present invention to provide the preparation method of said composition.
A kind of composition with antidepressant effect, it can be prepared by following methods：
Method one：Eleutheroside and rhodioside are commercially available, according to weight proportion, add conventional auxiliary material, are made.
Or prepared by following methods：Method two
(1) wilsonii medicinal material is cut into slices and refluxing extraction is carried out 2~5 times using the water of 10~16 times of amounts, each 1h, collection is carried
Take liquid and filtering and concentrating obtains medicinal extract；Eluted with ethanol alcohol precipitation with 10~50%7 alcohol twice, then through AB-8 types macroporous absorbent resin,
Collect the eleutheroside medicinal extract of eluent concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 2~5 times using the decocting of 6~10 times of amounts, each 2h is refiltered and is concentrated to give
Medicinal extract；Ethanol alcohol precipitation is used, the chromatographic column through polymer carrier stationary phase is entered using not higher than 10% methanol or ethanol water
Row elution, collects eluent and is concentrated to give sterling rhodioside medicinal extract；
(3) by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made；
It is preferred that,
(1) wilsonii medicinal material is cut into slices, refluxing extraction is carried out 3 times using the water of 14 times of amounts, each 1h collects three extractions
Simultaneously filtering and concentrating obtains medicinal extract to liquid；With ethanol alcohol precipitation twice, then through AB-8 30% ethanol elutions of type macroporous absorbent resin, collection is washed
The eleutheroside medicinal extract of de- liquid concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 3 times using the decocting of 8 times of amounts, each 2h is refiltered and is concentrated to give medicinal extract；With
Twice, the chromatographic column through polymer carrier stationary phase is carried out ethanol alcohol precipitation using not higher than 10% methanol or ethanol water
Elution, collects eluent and is concentrated to give sterling rhodioside medicinal extract；
(3) by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made.
Or prepared by following methods：Method three
1. wilsonii is crushed, is 50~75% ethanol, 30~50min of extraction time, ultrasonic power 120 with volume fraction
~180W, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；Ethanol alcohol precipitation is used, then through AB-8 type macroporous absorption trees
30% ethanol elution of fat, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, with the ethanol of volume fraction 50~75%, 30~45 DEG C of Extracting temperature, extraction time 30min,
Extract 3 times, collect No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, the color through polymer carrier stationary phase
Post is composed, is eluted using not higher than 10% methanol or ethanol water, eluent is collected and is concentrated to give the leaching of sterling rhodioside
Cream；
3. by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made；
It is preferred that,
1. wilsonii is crushed, is 60% ethanol, extraction time 40min, ultrasonic power 150W with volume fraction, extracts 3
It is secondary, collect No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, then through AB-8 type macroporous absorbent resins 30% is used
Ethanol elution, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, with the ethanol of volume fraction 60%, 30~45 DEG C of Extracting temperature, extraction time 30min, extracted
3 times, collect No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, the chromatographic column through polymer carrier stationary phase,
Eluted using not higher than 10% methanol or ethanol water, collect eluent and be concentrated to give sterling rhodioside medicinal extract；
3. by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made；
In this method, the ratio of wilsonii and ethanol is that every 1g medicinal materials add 6ml ethanol；The ratio of rhodiola root and ethanol is
20ml ethanol is added per 1g medicinal materials.
A kind of composition with antidepressant effect, it is characterised in that said composition can for tablet, pill, capsule,
Granule, injection, oral liquid.
A kind of composition with antidepressant effect, it is characterised in that said composition is applied to the preparation of antidepressant.
Experimental example
To better illustrate beneficial effects of the present invention, those skilled in the art have carried out experimental study.
The Tail suspension test of experimental example 1 (TST) and mouse forced swimming test (FST)
1.1 animal：
Normal male ICR mouse, purchased from the western pul-Bi Kai experimental animals Co., Ltd in Shanghai.
1.2 tested material：
Fluoxetine hydrochloride, purchased from Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4, lot number 20131220.
Eleutheroside I, purchased from Hubei Ju Sheng Science and Technology Ltd.s；
Rhodioside I, purchased from Hubei Ju Sheng Science and Technology Ltd.s.
Eleutheroside II, is prepared according to preparation method two of the present invention；
Rhodioside II, is prepared according to preparation method two of the present invention.
Eleutheroside III, is prepared according to preparation method three of the present invention；
Rhodioside III, is prepared according to preparation method three of the present invention.
1.3 experiment packet
G1 groups：Blank control group, gives physiological saline；
G2 groups：Model control group, gives physiological saline；
G3 groups：Positive controls, give Fluoxetine hydrochloride；
G4 groups：Experimental group, gives eleutheroside I or eleutheroside II or eleutheroside III；
G5 groups：Experimental group, gives rhodioside I or rhodioside II or rhodioside III；
G6 groups：Experimental group, gives eleutheroside I: rhodioside II (1: 1)；
G7 groups：Experimental group, gives eleutheroside II: rhodioside III (1: 2)；
G8 groups：Experimental group, gives eleutheroside III: rhodioside I (1: 3)；
G9 groups：Experimental group, gives eleutheroside I: rhodioside III (2: 1)；
G10 groups：Experimental group, gives eleutheroside II: rhodioside II (3: 1)；
G11 groups：Experimental group, gives eleutheroside III: rhodioside I (2: 3)；
G12 groups：Experimental group, gives eleutheroside I: rhodioside II (3: 2).
1.4 experimental method
Normal male mice 100 is chosen, body weight is 20 ± 4 grams, is randomly divided into blank control group, model control group, sun
Property control group (Fluoxetine hydrochloride) and experimental group, every group 10.Mouse open field test (OFT) is used after each mouse stomach administration 7d
False positive results (experimental result is shown in Table 1) are excluded, and pass through Tail suspension test (TST) and mouse forced swimming test (FST)
Study the antidepressant effect of the combination (experimental result is shown in Table 2).
1) Tail suspension test (TST)：
Mouse carries out outstanding tail test in behind successive administration 7 days after 21d modelings, the 7th day administration 1h.By single mouse tail end
(at away from tail point portion about 2cm) is fixed on geometrical clamp in outstanding boot upper bracket, makes into projecting state, head is from bottom about
5cm, mouse is in order to overcome abnormal position and struggle activity, but activity a period of time motionless, disappointed shape of display that discontinuity occurs
The state suspension time is 6min, hangs tail after statistics in 4min and adds up the dead time (motionless state is that mouse stops motionless or nothing of struggling
Any activity).Image/video is preserved, is analyzed and processed with Topscan softwares, when counting in rear 4min outstanding tail and adding up motionless
Between.
2) mouse forced swimming test (FST)：
Second day, i.e. mouse are administered the 8th day after outstanding tail, after administration 1h, carry out forced swim test.Mouse is independent
In the cylindrical glass cylinder for being put into high 20cm diameters 14cm, depth of water 10cm in cylinder, water temperature (25 ± 2) DEG C enters from mouse and counted after water
When 6min, the swimming accumulative dead time (refers to mouse and stops struggle, or display floating state in water, only have in 4min after record
Small limb motion is to keep head to keep afloat).Image/video is preserved, is analyzed and processed with Topscan softwares, is counted
Go out the accumulative dead time of swimming in rear 4min.
The different weight of table 1 proportioning eleutheroside+rhodioside passes through the influence of lined number and upright number of times to OFT
The different weight of table 2 matches the influence of eleutheroside+rhodioside TsT and FsT dead time
Note：Compared * P with model control group<0.05, * * P<0.01；Compared #P with Fluoxetine hydrochloride group<0.05, ##P<
0.01。
Result of study is shown, in TST and FST, and the dead time of each administration group mouse substantially reduces, wherein to pierce five
Plus glycosides+rhodioside with weight proportion 1: 2 form a team the mouse dead time influence it is the most obvious, with positive controls and blank pair
There is pole significant difference (P according to group<0.01).In addition eleutheroside+rhodioside with 2: 3 groups of weight proportion it is motionless to mouse when
Between influence more substantially, (the P that compared that there were significant differences with Normal group<0.05).This result illustrates different weight proportioning thorn five
Plus glycosides+rhodioside combination medicament has significantly antidepressant effect, but there is the difference of antidepression degree.
Influence of the experimental example 2 to depression rat monoamine neurotransmitter and its metabolin
1.1 animal：
Normal male rats, SPF grades, purchased from the western pul-Bi Kai experimental animals Co., Ltd in Shanghai.
1.2 tested material：
Fluoxetine hydrochloride, purchased from Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4, lot number 20131220.
Eleutheroside I, purchased from Hubei Ju Sheng Science and Technology Ltd.s；
Rhodioside I, purchased from Hubei Ju Sheng Science and Technology Ltd.s.
Eleutheroside II, is prepared according to preparation method two of the present invention；
Rhodioside II, is prepared according to preparation method two of the present invention.
Eleutheroside III, is prepared according to preparation method three of the present invention；
Rhodioside III, is prepared according to preparation method three of the present invention.
1.3 experiment packet
G1 groups：Blank control group, gives physiological saline；
G2 groups：Model control group, gives physiological saline；
G3 groups：Positive controls, give Fluoxetine hydrochloride；
G4 groups：Experimental group, gives eleutheroside I or eleutheroside II or eleutheroside III；
G5 groups：Experimental group, gives rhodioside I or rhodioside II or rhodioside III；
G6 groups：Experimental group, gives eleutheroside I: rhodioside II (1: 1)；
G7 groups：Experimental group, gives eleutheroside II: rhodioside III (1: 2)；
G8 groups：Experimental group, gives eleutheroside III: rhodioside I (1: 3)；
G9 groups：Experimental group, gives eleutheroside I: rhodioside III (2: 1)；
G10 groups：Experimental group, gives eleutheroside II: rhodioside II (3: 1)；
G11 groups：Experimental group, gives eleutheroside III: rhodioside I (2: 3)；
G12 groups：Experimental group, gives eleutheroside I: rhodioside II (3: 2).
1.4 experimental method
250 ± 20 grams of rats are chosen, blank control group, model control group, positive controls (hydrochloric acid fluorine west is randomly divided into
Spit of fland) and experimental group, every group 10, sub-cage rearing.Model control group, positive controls and experimental group carry out 21 days chronic milds not
Predictable stress stimulation：Electric shock vola (current strength 1mA, voltage 45mV, 10s/ times, be spaced 1min, totally 30 times), frozen water trip
Swim (4 DEG C, 5min), rock (1 time/s, 15min), folder tail (3min), heat stress (45 DEG C, 5min), fasting+noise, taboo water+different
Thing, reverse round the clock, constraint (8h), fasting+humidity bedding and padding (adding 200ml water in per 100g bedding and padding) and taboo water+inclination mouse cage.Often
1 kind of stimulation mode of its random arrangement, every kind of stimulation is used no more than 3 times in experimentation.Rats in normal control group is refused to appoint
What is stimulated.During modeling, Normal group and the daily gavage 50ml physiological saline of model control group, positive controls and experiment
Group, is administered, is administered once a day, each 50mg/kg for continuous 21 days.By manufacturing chronic stress depression rat model, and lead to
Cross body weight and Open field activity excludes false positive results, reuse in high performance liquid chromatography (HPLC) method detection each group rat cerebral tissue
The antidepression function of monoamine neurotransmitter and its metabolite content Changeement eleutheroside+rhodioside (be shown in by experimental result
Table 3 and table 4).
The variance analysis of body weight and the motion of modeling anterior-posterior horizontal before the treatment of each group of table 3
The influence of rat cerebral tissue's prefrontal lobe monoamine neurotransmitter and its metabolin after the treatment of each group of table 4
Note：Compared * P with model control group<0.05, * * P<0.01；Compared #P with Fluoxetine hydrochloride group<0.05, ##P<
0.01。
Result of study shows, homovanillic acid (HVA), norepinephrine (NE), dopamine in depression model group prefrontal lobe
(DA), the content of serotonin (5-HT) is above blank control group, thus illustrates in rat chronic stress depression and brain tissue
Prefrontal lobe monoamine neurotransmitter and its metabolite content change are relevant.In addition, compared with model group, eleutheroside+rhodiola root
Glycosides (1: 2；2: 3) DA, 5-HT are substantially reduced in rat cerebral tissue's prefrontal lobe in combination medicament administration group and positive controls, are said
Bright eleutheroside+rhodioside combination medicament has significant antidepression function.
The tablet of embodiment 1
The rhodioside 5 of eleutheroside 3
Above-mentioned raw materials are commercially available, are mixed by the parts by weight, add appropriate amount of starch and magnesium stearate, granulation, whole grain, pressure
Piece, is produced.
The hard capsule of embodiment 2
The rhodioside 4 of eleutheroside 7
Above-mentioned raw materials are commercially available, are mixed by the parts by weight, add appropriate amount of starch, are pelletized with polyacrylic resin, fill glue
Capsule, is produced.
The soft capsule of embodiment 3
The rhodioside 8 of eleutheroside 5
Above-mentioned raw materials are commercially available, are mixed by the parts by weight, add the miscella that corn oil, soybean lecithin, beeswax are made
Appropriate matrix, is sufficiently mixed, and with colloid barreling to uniform, using pressing, soft capsule is made, produces.
The granule of embodiment 4
The rhodioside 9 of eleutheroside 2
Above-mentioned raw materials are commercially available, are mixed by the parts by weight, add 30% microcrystalline cellulose, 1% magnesium stearate, 4% friendship
Join sodium carboxymethylcellulose, granulation, whole grain are produced.
The granule of embodiment 5
The rhodioside 2 of eleutheroside 9
Above-mentioned raw materials are commercially available, are mixed by the parts by weight, add 30% microcrystalline cellulose, 1% magnesium stearate, 4% friendship
Join sodium carboxymethylcellulose, granulation, whole grain are produced.
The pill of embodiment 6
The rhodioside 1 of eleutheroside 1
(1) wilsonii medicinal material is cut into slices and refluxing extraction is carried out 5 times using the water of 10 times of amounts, each 1h collects extract solution simultaneously
Filtering and concentrating obtains medicinal extract；Eluted with ethanol alcohol precipitation with 50%7 alcohol twice, then through AB-8 types macroporous absorbent resin, collect eluent
The eleutheroside medicinal extract of concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 5 times using the decocting of 6 times of amounts, each 2h is refiltered and is concentrated to give medicinal extract；With
Ethanol alcohol precipitation, the chromatographic column through polymer carrier stationary phase is eluted using not higher than 10% methanol or ethanol water,
Collect eluent and be concentrated to give sterling rhodioside medicinal extract；
(3) gained eleutheroside medicinal extract, rhodioside medicinal extract are mixed according to weight proportion, sieving, adds appropriate poly- second
Glycol 4000, heating fusing, is mixed, and is instilled in methyl-silicone oil cooling agent, pill, film coating is produced.
The oral liquid of embodiment 7
The rhodioside 2 of eleutheroside 1
(1) wilsonii medicinal material is cut into slices and refluxing extraction is carried out 2 times using the water of 16 times of amounts, each 1h collects extract solution simultaneously
Filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, then through AB-8 10% ethanol elutions of type macroporous absorbent resin, eluent is collected
The eleutheroside medicinal extract of concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 2 times using the decocting of 10 times of amounts, each 2h is refiltered and is concentrated to give medicinal extract；
Ethanol alcohol precipitation is used, the chromatographic column through polymer carrier stationary phase is washed using not higher than 10% methanol or ethanol water
It is de-, collect eluent and be concentrated to give sterling rhodioside medicinal extract；
(3) gained eleutheroside medicinal extract, rhodioside medicinal extract are mixed according to weight proportion, adds appropriate Icing Sugar, stevia rebaudianum
Element, tween, purified water dissolving, are produced.
The water-honeyed pill of embodiment 8
The rhodioside 3 of eleutheroside 1
(1) wilsonii medicinal material is cut into slices and refluxing extraction is carried out 3 times using the water of 14 times of amounts, each 1h collects extract solution simultaneously
Filtering and concentrating obtains medicinal extract；Eluted with ethanol alcohol precipitation with 30%7 alcohol twice, then through AB-8 types macroporous absorbent resin, collect eluent
The eleutheroside medicinal extract of concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 3 times using the decocting of 8 times of amounts, each 2h is refiltered and is concentrated to give medicinal extract；With
Ethanol alcohol precipitation, the chromatographic column through polymer carrier stationary phase is eluted using not higher than 10% methanol or ethanol water,
Collect eluent and be concentrated to give sterling rhodioside medicinal extract；
(3) by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, drying and crushing is mixed, per 100g powder
End plus refined honey 40g, with appropriate boiling water, general ball is dried, produced.
The water-honeyed pill of embodiment 9
The rhodioside 10 of eleutheroside 1
1. the wilsonii, (g of solid-liquid ratio 1: 10 are crushed：ML), it is 50% ethanol, extraction time 50min, ultrasound with volume fraction
Wave power 180W, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；Ethanol alcohol precipitation is used, then is inhaled through AB-8 types macropore
30% ethanol elution of attached resin, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, (the g of solid-liquid ratio 1: 25：ML), with the ethanol of volume fraction 50%, 45 DEG C of Extracting temperature, extraction
Time 30min, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, through polymer carrier
The chromatographic column of stationary phase, is eluted using not higher than 10% methanol or ethanol water, is collected eluent and is concentrated to give sterling
Rhodioside medicinal extract；
3. by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, drying and crushing is mixed, per 100g powder
End plus refined honey 40g, with appropriate boiling water, general ball is dried, produced.
The tablet of embodiment 10
The rhodioside 1 of eleutheroside 10
1. the wilsonii, (g of solid-liquid ratio 1: 5 are crushed：ML), it is 75% ethanol, extraction time 30min, ultrasound with volume fraction
Wave power 120W, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；Ethanol alcohol precipitation is used, then is inhaled through AB-8 types macropore
30% ethanol elution of attached resin, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, (the g of solid-liquid ratio 1: 15：ML), with the ethanol of volume fraction 75%, 30 DEG C of Extracting temperature, extraction
Time 30min, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, through polymer carrier
The chromatographic column of stationary phase, is eluted using not higher than 10% methanol or ethanol water, is collected eluent and is concentrated to give sterling
Rhodioside medicinal extract；
3. gained eleutheroside medicinal extract, rhodioside medicinal extract are mixed according to weight proportion, adds 30% microcrystalline cellulose
Element, 1% magnesium stearate, 4% Ac-Di-Sol, granulation, whole grain, tabletting are produced.
The granule of embodiment 11
The rhodioside 3 of eleutheroside 8
1. the wilsonii, (g of solid-liquid ratio 1: 8 are crushed：ML), it is 65% ethanol, extraction time 40min, ultrasound with volume fraction
Wave power 150W, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；Ethanol alcohol precipitation is used, then is inhaled through AB-8 types macropore
30% ethanol elution of attached resin, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, (the g of solid-liquid ratio 1: 15：ML), with the ethanol of volume fraction 65%, 40 DEG C of Extracting temperature, extraction
Time 30min, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, through polymer carrier
The chromatographic column of stationary phase, is eluted using not higher than 10% methanol or ethanol water, is collected eluent and is concentrated to give sterling
Rhodioside medicinal extract；
3. gained eleutheroside medicinal extract, rhodioside medicinal extract are mixed according to weight proportion, adds 30% microcrystalline cellulose
Element, 1% magnesium stearate, 4% Ac-Di-Sol, granulation, whole grain, are produced.
Claims (18)
1. a kind of composition with antidepressant effect, it is characterised in that said composition is consisted of the following composition：Eleutheroside with
The weight ratio of rhodioside is 1~10: 1~10, and said composition is applied to the preparation of antidepressant.
2. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 1: 10, and said composition is applied to the preparation of antidepressant.
3. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 10: 1, and said composition is applied to the preparation of antidepressant.
4. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 1: 9, and said composition is applied to the preparation of antidepressant.
5. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 9: 1, and said composition is applied to the preparation of antidepressant.
6. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 1: 2, and said composition is applied to the preparation of antidepressant.
7. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 1: 3, and said composition is applied to the preparation of antidepressant.
8. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 2: 3, and said composition is applied to the preparation of antidepressant.
9. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 3: 2, and said composition is applied to the preparation of antidepressant.
10. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 3: 1, and said composition is applied to the preparation of antidepressant.
11. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Wilsonii
The weight ratio of glycosides and rhodioside is 5: 8, and said composition is applied to the preparation of antidepressant.
12. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 8: 3, and said composition is applied to the preparation of antidepressant.
13. a kind of composition as claimed in claim 1, it is characterised in that said composition is consisted of the following composition：Eleutheroside
Weight ratio with rhodioside is 7: 4, and said composition is applied to the preparation of antidepressant.
14. a kind of preparation method of composition as claimed in claim 1, it is characterised in that said composition uses following methods system
It is standby：
Method one：Eleutheroside and rhodioside are commercially available, according to weight proportion, add conventional auxiliary material, are made；
Method two：(1) wilsonii medicinal material is cut into slices and refluxing extraction is carried out 2~5 times using the water of 10~16 times of amounts, each 1h is received
Simultaneously filtering and concentrating obtains medicinal extract to collection extract solution；With ethanol alcohol precipitation twice, then through AB-8 types macroporous absorbent resin with 10~50% ethanol
Elution, collects the eleutheroside medicinal extract of eluent concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 2~5 times using the decocting of 6~10 times of amounts, each 2h is refiltered and is concentrated to give leaching
Cream；Ethanol alcohol precipitation is used, the chromatographic column through polymer carrier stationary phase is carried out using not higher than 10% methanol or ethanol water
Elution, collects eluent and is concentrated to give sterling rhodioside medicinal extract；
(3) by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made；
Method three：1. wilsonii is crushed, is 50~75% ethanol, 30~50min of extraction time, ultrasonic power with volume fraction
120~180W, is extracted 3 times, collects No. three extract solutions and filtering and concentrating obtains medicinal extract；Ethanol alcohol precipitation is used, then is inhaled through AB-8 types macropore
30% ethanol elution of attached resin, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, with the ethanol of volume fraction 50~75%, 30~45 DEG C of Extracting temperature, extraction time 30min, extracted
3 times, collect No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, the chromatographic column through polymer carrier stationary phase,
Eluted using not higher than 10% methanol or ethanol water, collect eluent and be concentrated to give sterling rhodioside medicinal extract；
3. by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made.
15. preparation method as claimed in claim 14, its feature is in method two,
(1) wilsonii medicinal material is cut into slices, refluxing extraction is carried out 3 times using the water of 14 times of amounts, each 1h collects No. three extract solutions simultaneously
Filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, then through AB-8 30% ethanol elutions of type macroporous absorbent resin, eluent is collected
The eleutheroside medicinal extract of concentration；
(2) by rhodiola root pulverizing medicinal materials, boiled 3 times using the decocting of 8 times of amounts, each 2h is refiltered and is concentrated to give medicinal extract；Use ethanol
Twice, the chromatographic column through polymer carrier stationary phase is eluted alcohol precipitation using not higher than 10% methanol or ethanol water,
Collect eluent and be concentrated to give sterling rhodioside medicinal extract；
(3) by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made.
16. preparation method as claimed in claim 14, it is characterised in that method three is,
1. wilsonii is crushed, is 60% ethanol, extraction time 40min, ultrasonic power 150W with volume fraction, extracts 3 times, receive
Simultaneously filtering and concentrating obtains medicinal extract to No. three extract solutions of collection；With ethanol alcohol precipitation twice, then through AB-8 30% ethanol of type macroporous absorbent resin
Elution, collects the eleutheroside medicinal extract of eluent concentration；
2. rhodiola root is crushed, with the ethanol of volume fraction 60%, 30~45 DEG C of Extracting temperature, extraction time 30min, extracted 3 times,
Collect No. three extract solutions and filtering and concentrating obtains medicinal extract；With ethanol alcohol precipitation twice, the chromatographic column through polymer carrier stationary phase, is used
Not higher than 10% methanol or ethanol water is eluted, and is collected eluent and is concentrated to give sterling rhodioside medicinal extract；
3. by gained eleutheroside medicinal extract, rhodioside medicinal extract according to weight proportion, conventional auxiliary material is added, is made.
17. preparation method as claimed in claim 16, it is characterised in that in method three, the ratio of wilsonii and ethanol is every 1g
Medicinal material adds 5~10ml ethanol；The ratio of rhodiola root and ethanol is that every 1g medicinal materials add 15~25ml ethanol.
18. the composition as described in any one of claim 1~13, it is characterised in that said composition can be tablet, pill, glue
Wafer, granule, injection, oral liquid.
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| CN117180292A (en) * | 2022-07-19 | 2023-12-08 | 北京斯高科技有限公司 | Salidroside or rhodiola rosea extract for preparing medicine for treating heart block |
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| CN1531954A (en) * | 2003-03-19 | 2004-09-29 | 成都康弘科技实业（集团）有限公司 | Medicine for treating depression and its preparing method |
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| CN1531954A (en) * | 2003-03-19 | 2004-09-29 | 成都康弘科技实业（集团）有限公司 | Medicine for treating depression and its preparing method |
| CN1593226A (en) * | 2004-06-30 | 2005-03-16 | 虞泓 | Health food |
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