CN104031976A - Method for detecting number of lactic acid bacteria in fermented milk - Google Patents
Method for detecting number of lactic acid bacteria in fermented milk Download PDFInfo
- Publication number
- CN104031976A CN104031976A CN201410267349.1A CN201410267349A CN104031976A CN 104031976 A CN104031976 A CN 104031976A CN 201410267349 A CN201410267349 A CN 201410267349A CN 104031976 A CN104031976 A CN 104031976A
- Authority
- CN
- China
- Prior art keywords
- milk
- fermented
- value
- acid bacteria
- difference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to the field of analytical chemistry and discloses a method for detecting number of lactic acid bacteria in fermented milk. The method comprises the following steps: diluting a fermented milk standard substance into 5-20 times as much as before by using UHT milk, adding resazurin for reacting, recording R difference between a final R value and an initial R value of the fermented milk standard substance by using a red, green and blue (RGB) color information acquisition instrument, and establishing a standard equation between the R difference X and total number Y of the lactic acid bacteria colonies; replacing the fermented milk standard substance as a to-be-detected sample of the fermented milk; recording the R difference of the to-be-detected sample of the fermented milk and substituting the R difference into the acquired standard equation in the same detection environment, and detecting the lactic acid bacteria number in the to-be-detected sample of the fermented milk. The fermented milk sample is diluted and adjusted by utilizing the UHT milk, so that the sample accords with the reaction conditions of the resazurin reduction method and the RGB color information acquisition principle, the same dilution ratio between the samples is guaranteed, the method does not have obvious difference in detection result as compared with a standard method, and the accuracy of the result is greatly guaranteed on the basis of rapid detection.
Description
Technical field
The present invention relates to analytical chemistry field, relate in particular a kind of method that detects lactic acid bacteria number in fermented-milk.
Background technology
Fermented-milk is defined as " take raw ox (sheep) breast or milk powder is raw material, the product of the pH value reduction of making after sterilization, fermentation " in GB19302-2010, has contained the products such as fermented-milk, yogurt, flavored fermented milk, local flavor yogurt.Viable count when edible in fermented-milk and leben need reach some amount and just can play a role in health care, so in GB19302-2010, has clearly stipulated that the milk-acid bacteria of fermented milk prod limits the quantity of: lactic acid bacteria number>=1 * 10
6cfu/g (ml).
Milk-acid bacteria adds up to the crucial test item of fermented milk prod factory testing, in industry, to the detection means of fermented-milk milk-acid bacteria sum, be GB4789.35 method at present, the method not only needs professional to operate, running cost is higher, and most importantly the round of visits of the method is 48 hours, because fermented-milk is full cold chain transportation, and it sells the quality guaranteed period very short (15 days), originally very short shelf-lives of fermented milk prod has seriously been shortened in very long factory inspection, the serious profit that affects enterprise.
In order to shorten detection time, prior art is reported based on resazurin reduction method, the RGB colouring information acquisition instrument of employing medicine and hygiene fields, and record detects color sample to be changed, and it is relevant with total plate count to detect color sample variation, thus obtain total plate count.But, due to the pH of fermented milk prod too low (commercially available qualified fermented-milk pH value is in 4.0～4.5 scopes), do not meet the reaction conditions of resazurin reducing dyes method.Correlative study result proves, application resazurin reducing dyes method detects milk-acid bacteria sum, pH value in 6.5～7.0 scopes detected result without significant difference.Therefore fermented-milk sample need to be made after treatment its pH value in 6.5～7.0 scopes, can apply resazurin reducing dyes method its milk-acid bacteria sum is detected.
Conventional pH value control method is adjusted to required pH value scope for sample is added to the higher damping fluid of pH value, although this method consumption is less, but because the pH value of fermented milk prod is not fixed value, therefore apply damping fluid and be adjusted to the dilute strength difference that same pH value can make sample, thereby cause detected result inaccurate.
If add neutral pure water or deionized water dilution, can make sample pH value value reach within the scope of 6.5-7.0, but need to carry out 100 times of above dilutions to fermented-milk sample, thereby reduced greatly accuracy of detection, what is more important, the principle gathering according to RGB colouring information, it is for to gather colour-change information by LED light to the reflection of sample, if by nearly 100 times of diluted sample, sample diluting liquid is almost completely transparent, because sample reduces greatly to reflection of light, cannot accurately gather colour-change information, cannot detect accurately.
Summary of the invention
In view of this, the invention provides a kind of method that detects lactic acid bacteria number in fermented-milk, make described method can detect fast and accurately the lactic acid bacteria number in fermented-milk.
For achieving the above object, the invention provides following technical scheme:
A method that detects lactic acid bacteria number in fermented-milk, comprises the steps:
Step 1, by fermented-milk standard substance with UHT breast dilution 5-20 doubly, then in the fermented-milk standard substance after dilution, add resazurin reaction, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value and initial R value, and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Wherein, described terminal R value is the R value of random time point in 1-15 minute, and described starting point R value is the R value of 0 minute;
Step 2, fermented-milk standard substance in step 1 are replaced with to fermented-milk testing sample, under identical testing environment, record in the standard equation obtaining in the R difference of fermented-milk testing sample substitution step 1, detect the lactic acid bacteria number in fermented-milk testing sample.
Milk-acid bacteria is in metabolic process, because physiological requirements produce oxydo-reductase, this enzyme and react and make it become pink colour from blueness with resazurin, and how much directly related number and the lactic acid bacteria number of oxydo-reductase be, and then the colour-change of reacting by instrument record (variation of R value), set up the formula relevant with lactic acid bacteria number.
UHT of the present invention (Ultra High Temperature treated) breast is ultra-high-temperature sterilized milk.Ultra High Temperature Short Time, 135-140 ℃, 4-10 second, is a kind of sterilization process that fresh milk is processed.The present invention adopts UHT breast to dilute sample, and the first, it can not bring bacteria effect detected result into; The second, it can not change the transparency of solution, and the sample of processing is still opaque milky white liquid; Therefore the 3rd, UHT breast is electronegativity colloidal solution, and electronegativity micelle will adsorb hydrogen ion, and under the identical prerequisite of dilution consumption, the sample pH value value of UHT breast dilution is higher, therefore use UHT breast regulate sample pH value value to 6.5-7.0 consumption compared with pure water still less.
The present invention adopts respectively 8 times, 10 times and 15 times of UHT breast dilution fermented-milks, and the fermented-milk pH value after dilution is all within the scope of 6.5-6.9.
In technical solution of the present invention, preferably, described fermented-milk standard substance number is more than 5.
Preferably, described resazurin concentration is 0.0005-0.05g/mL.
Preferably, the volume ratio of the fermented-milk standard substance after described dilution and resazurin is 9:1.
Preferably, described extension rate is 8-15 times, and more preferably, described extension rate is 10 times.
Preferably, described terminal R value is the R value of the 5th minute.
Gather 12 commercially available fermented-milk samples, according to the method for the invention, detect, whole testing process only needs 5min, and detected result and GB4789.35 detected result contrast and carry out t check, result demonstration and this area standard detecting method GB4789.35, without significant difference, show the pin-point accuracy that the method for the invention detects.
From above technical scheme, the present invention utilizes UHT breast to dilute adjustment to fermented-milk sample, make reaction conditions and the RGB colouring information acquisition principle of samples met resazurin reduction method, guaranteed between sample, to there is identical extension rate simultaneously, without significant difference, on the basis of rapid detection, guaranteed greatly the accuracy of result with GB4789.35 detected result.
Embodiment
The invention discloses a kind of method that detects lactic acid bacteria number in fermented-milk, those skilled in the art can use for reference content herein, suitably improve processing parameter and realize.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in the present invention.Method of the present invention is described by preferred embodiment, and related personnel obviously can change methods and applications as herein described or suitably change and combination within not departing from content of the present invention, spirit and scope, realizes and apply the technology of the present invention.
Below in conjunction with embodiment, further set forth the present invention.
Embodiment 1:UHT breast dilution fermented-milk sample
Get a plurality of fermented-milk samples, with UHT breast, it is diluted to 8 times, 10 times and 15 times respectively, each sample pH value value is in Table 1.
Table 1 dilution secondary fermentation breast sample pH value value
| PH value before dilution | 4.31 | 4.42 | 4.33 | 4.54 | 4.27 |
| PH value after diluting 8 times | 6.60 | 6.64 | 6.53 | 6.74 | 6.50 |
| PH value before dilution | 4.53 | 4.11 | 4.15 | 4.21 | 4.13 |
| PH value after diluting 10 times | 6.80 | 6.74 | 6.74 | 6.74 | 6.76 |
| PH value before dilution | 4.30 | 4.44 | 4.24 | 4.24 | 4.36 |
| PH value after diluting 10 times | 6.73 | 6.91 | 6.75 | 6.71 | 6.83 |
| PH value before dilution | 4.77 | 4.87 | 4.60 | 4.60 | 4.51 |
| PH value after diluting 15 times | 6.82 | 6.84 | 6.87 | 6.81 | 6.77 |
As shown in Table 1, the present invention adopts respectively 8 times, 10 times and 15 times of UHT breast dilution fermented-milks, 8 times, 10 times and 15 times of UHT breast dilution fermented-milks, and its pH value is all within the scope of 6.5-6.9.
Embodiment 2: the method for lactic acid bacteria number in detection fermented-milk of the present invention
5 fermented-milk standard substance are diluted to 10 times with UHT breast respectively, then get respectively fermented-milk standard substance after 1800 μ L dilutions in cuvette, add 0.005g/mL resazurin solution 200 μ L to mix, 10 fermented-milk standard substance are detected, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value (the R value of the 10th minute) and initial R value (the R value of 0 minute), and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y, Y=4.7985X-278.87 (R
2=0.9013);
Above-mentioned fermented-milk standard substance are replaced with to fermented-milk testing sample, under identical testing environment, record in the R difference substitution standard equation of fermented-milk testing sample, detect the lactic acid bacteria number in fermented-milk testing sample.
Gather 12 commercially available fermented-milk samples, with the present embodiment method, sample is detected and show that R difference X brings Y=4.7985X-278.87 into, obtain milk-acid bacteria sum, and contrast with GB4789.35 detected result, t checks (two tail), the results are shown in Table 2 and table 3.
Table 2 method of the present invention and GB4789.35 detected result
The inventive method in table 2 and GB4789.35 method measurement result are carried out to t-check, the results are shown in Table 3.
Table 3t-assay
| t?Start | The two tails of P (T≤t) | T (two tails are critical) |
| -1.17 | 0.27 | 2.20 |
From table 3t-assay, t Stat<t (two tails are critical), illustrates that lactic acid bacteria number and the GB4789.35 method detected result difference of the method for the invention detection is not remarkable.
Embodiment 3: the method for lactic acid bacteria number in detection fermented-milk of the present invention
5 fermented-milk standard substance are diluted to 8 times with UHT breast respectively, then get respectively fermented-milk standard substance after 1800 μ L dilutions in cuvette, add 0.001g/mL resazurin solution 200 μ L to mix, 5 fermented-milk standard substance are detected, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value (the R value of the 5th minute) and initial R value (the R value of 0 minute), and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Above-mentioned fermented-milk standard substance are replaced with to fermented-milk testing sample, under identical testing environment, record in the R difference substitution standard equation of fermented-milk testing sample, detect the lactic acid bacteria number in fermented-milk testing sample.
With reference to the simultaneous test in embodiment 1,12 commercially available fermented-milk samples of same collection, with the present embodiment method, sample is detected and show that R difference X brings in standard equation, obtain milk-acid bacteria sum, and contrast with GB4789.35 detected result, t checks (two tail), and result shows, t Stat<t (two tails are critical), illustrates that lactic acid bacteria number and the GB4789.35 method detected result difference of the method for the invention detection is not remarkable.
Embodiment 4: the method for lactic acid bacteria number in detection fermented-milk of the present invention
10 fermented-milk standard substance are diluted to 13 times with UHT breast respectively, then get respectively fermented-milk standard substance after 1800 μ L dilutions in cuvette, add 0.0005g/mL resazurin solution 200 μ L to mix, 10 fermented-milk standard substance are detected, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value (the R value of the 10th minute) and initial R value (the R value of 0 minute), and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Above-mentioned fermented-milk standard substance are replaced with to fermented-milk testing sample, under identical testing environment, record in the R difference substitution standard equation of fermented-milk testing sample, detect the lactic acid bacteria number in fermented-milk testing sample.
With reference to the simultaneous test in embodiment 1,12 commercially available fermented-milk samples of same collection, with the present embodiment method, sample is detected and show that R difference X brings in standard equation, obtain milk-acid bacteria sum, and contrast with GB4789.35 detected result, t checks (two tail), and result shows, t Stat<t (two tails are critical), illustrates that lactic acid bacteria number and the GB4789.35 method detected result difference of the method for the invention detection is not remarkable.
Embodiment 5: the method for lactic acid bacteria number in detection fermented-milk of the present invention
20 fermented-milk standard substance are diluted to 15 times with UHT breast respectively, then get respectively fermented-milk standard substance after 1800 μ L dilutions in cuvette, add 0.003g/mL resazurin solution 200 μ L to mix, 20 fermented-milk standard substance are detected, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value (the R value of the 15th minute) and initial R value (the R value of 0 minute), and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Above-mentioned fermented-milk standard substance are replaced with to fermented-milk testing sample, under identical testing environment, record in the R difference substitution standard equation of fermented-milk testing sample, detect the lactic acid bacteria number in fermented-milk testing sample.
With reference to the simultaneous test in embodiment 1,12 commercially available fermented-milk samples of same collection, with the present embodiment method, sample is detected and show that R difference X brings in standard equation, obtain milk-acid bacteria sum, and contrast with GB4789.35 detected result, t checks (two tail), and result shows, t Stat<t (two tails are critical), illustrates that lactic acid bacteria number and the GB4789.35 method detected result difference of the method for the invention detection is not remarkable.
Embodiment 6: the method for lactic acid bacteria number in detection fermented-milk of the present invention
4 fermented-milk standard substance are diluted to 13 times with UHT breast respectively, then get respectively fermented-milk standard substance after 1800 μ L dilutions in cuvette, add 0.002g/mL resazurin solution 200 μ L to mix, 4 fermented-milk standard substance are detected, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value (the R value of the 4th minute) and initial R value (the R value of 0 minute), and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Above-mentioned fermented-milk standard substance are replaced with to fermented-milk testing sample, under identical testing environment, record in the R difference substitution standard equation of fermented-milk testing sample, detect the lactic acid bacteria number in fermented-milk testing sample.
With reference to the simultaneous test in embodiment 1,12 commercially available fermented-milk samples of same collection, with the present embodiment method, sample is detected and show that R difference X brings in standard equation, obtain milk-acid bacteria sum, and contrast with GB4789.35 detected result, t checks (two tail), and result shows, t Stat<t (two tails are critical), illustrates that lactic acid bacteria number and the GB4789.35 method detected result difference of the method for the invention detection is not remarkable.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a method that detects lactic acid bacteria number in fermented-milk, is characterized in that, comprises the steps:
Step 1, by fermented-milk standard substance with UHT breast dilution 5-20 doubly, then in the fermented-milk standard substance after dilution, add resazurin reaction, with RGB colouring information acquisition instrument, record the R difference of fermented-milk standard substance terminal R value and initial R value, and set up the standard equation between R difference X and milk-acid bacteria total number of bacterial colony Y;
Wherein, described terminal R value is the R value of random time point in 1-15 minute, and described starting point R value is the R value of 0 minute;
Step 2, fermented-milk standard substance in step 1 are replaced with to fermented-milk testing sample, under identical testing environment, record in the standard equation obtaining in the R difference of fermented-milk testing sample substitution step 1, detect the lactic acid bacteria number in fermented-milk testing sample.
2. method according to claim 1, is characterized in that, described fermented-milk standard substance number is more than 5.
3. method according to claim 1, is characterized in that, described resazurin concentration is 0.0005-0.05g/mL.
4. method according to claim 1, is characterized in that, described extension rate be 8-15 doubly.
5. method according to claim 4, is characterized in that, described extension rate is 10 times.
6. method according to claim 1, is characterized in that, described terminal R value is the R value of the 5th minute.
7. method according to claim 1, is characterized in that, the fermented-milk standard substance after described dilution and the volume ratio of resazurin are 9:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267349.1A CN104031976B (en) | 2014-06-16 | 2014-06-16 | A kind of detect the method for lactic acid bacteria number in fermentation milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267349.1A CN104031976B (en) | 2014-06-16 | 2014-06-16 | A kind of detect the method for lactic acid bacteria number in fermentation milk |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104031976A true CN104031976A (en) | 2014-09-10 |
| CN104031976B CN104031976B (en) | 2016-08-31 |
Family
ID=51462971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410267349.1A Active CN104031976B (en) | 2014-06-16 | 2014-06-16 | A kind of detect the method for lactic acid bacteria number in fermentation milk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104031976B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557014A (en) * | 2019-01-24 | 2019-04-02 | 广东产品质量监督检验研究院（国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室） | A kind of method of lactic acid bacteria number in quick detection acidified milk |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0704538A1 (en) * | 1993-06-14 | 1996-04-03 | HE, Lin | A bacteria identification method and apparatus |
| WO2009032987A1 (en) * | 2007-09-05 | 2009-03-12 | Microbia, Inc. | Isolation of pellet-forming microorganisms |
-
2014
- 2014-06-16 CN CN201410267349.1A patent/CN104031976B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0704538A1 (en) * | 1993-06-14 | 1996-04-03 | HE, Lin | A bacteria identification method and apparatus |
| WO2009032987A1 (en) * | 2007-09-05 | 2009-03-12 | Microbia, Inc. | Isolation of pellet-forming microorganisms |
Non-Patent Citations (6)
| Title |
|---|
| 刘娜等: "刃天青还原法快速检测鲜牛奶中总菌数的研究", 《河南工业大学学报》, vol. 34, no. 1, 28 February 2013 (2013-02-28), pages 73 - 76 * |
| 吕加平等: "发酵乳品中乳酸菌生物量测定方法的研究", 《试验研究》, no. 2, 31 December 1997 (1997-12-31), pages 3 - 7 * |
| 富鑫: "生乳细菌快速检测技术研究与设备开发", 《东北农业大学硕士论文集》, 6 April 2011 (2011-04-06) * |
| 富鑫等: "一种发酵乳中乳酸菌总数快速检测技术的研究", 《食品研究与开发》, vol. 36, no. 4, 28 February 2015 (2015-02-28), pages 142 - 144 * |
| 王丽等: "利用色差法检测乳品菌群浓度的研究", 《哈尔滨理工大学学报》, vol. 9, no. 5, 31 October 2004 (2004-10-31), pages 46 - 48 * |
| 蔡强等: "色差法在乳品菌群浓度检测中的应用", 《计量与测试技术》, vol. 32, no. 12, 31 December 2005 (2005-12-31), pages 26 - 29 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557014A (en) * | 2019-01-24 | 2019-04-02 | 广东产品质量监督检验研究院（国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室） | A kind of method of lactic acid bacteria number in quick detection acidified milk |
| CN109557014B (en) * | 2019-01-24 | 2021-08-27 | 广东产品质量监督检验研究院（国家质量技术监督局广州电气安全检验所、广东省试验认证研究院、华安实验室） | Method for rapidly detecting number of lactic acid bacteria in fermented milk |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104031976B (en) | 2016-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gomes et al. | Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal | |
| CN103614451A (en) | Method for detecting microorganisms of cosmetics | |
| CN103642891A (en) | Method for inspecting microbes in cosmetics | |
| Ferreira et al. | Effect of high-pressure and thermal pasteurization on microbial and physico-chemical properties of Opuntia ficus-indica juices | |
| Ubeira-Iglesias et al. | High hydrostatic pressure processing of beetroot juice: Effects on nutritional, sensory and microbiological quality | |
| Mari et al. | Qualitative and guantitative validation of the micro biological survey method for Listeria spp., Salmonella spp., Enterobacteriaceae and Staphylococcus aureus in food samples | |
| CN101970682A (en) | Method for detecting and/or identifying clostridium difficile | |
| KR101459577B1 (en) | Qualitative antibiotics residue detection media | |
| CN104031976A (en) | Method for detecting number of lactic acid bacteria in fermented milk | |
| CN106350571B (en) | A kind of method of the sensitive antibiotics of quick screening treatment mastitis for milk cows | |
| CN103983626A (en) | Reagent for detecting lipase and method for rapidly detecting lipase | |
| Wright et al. | MacConkey broth purple provides an efficient MPN estimation method for Shigatoxigenic Escherichia coli | |
| KR20180070821A (en) | Method of manufacturing fermented meat products, fermented meat products produced therefrom | |
| CN107287275B (en) | Culture medium, kit containing culture medium and application of culture medium | |
| CN103168849A (en) | Direct vat set yogurt starter | |
| RU2701343C1 (en) | Nutrient medium for detection of lactic acid bacteria (versions) | |
| de SOUZA et al. | Evaluation of Petrifilm™ system compared with traditional methodology in count of indicators of sanitary-hygienic quality and pathogenic microorganisms in sheep milk | |
| CN102076868A (en) | Culture medium containing a spore germination inhibiting or delaying compound | |
| KR100955884B1 (en) | Method for detection of Enterobacter sakazakii using salicin and medium for detection of Enterobacter sakazakii | |
| CN103760122A (en) | Ultraviolet visible spectrophotometric method of escherichia coli | |
| JP5397991B2 (en) | Determination method of vitamin B12 using lactic acid bacteria | |
| Ali et al. | Physico-chemical characteristics of russian tea fungus: kombucha | |
| Teramura et al. | MC-Media Pad ACplus™ for Enumeration of Aerobic Counts in a Variety of Foods | |
| Akter et al. | Raw Milk In Noakhali, Bangladesh: Quality Assessment and Antibiotic Resistance of Identified Microorganisms. | |
| TW202009300A (en) | Time-temperature indicator for monitoring microbial spoilage of refrigerated food and manufacturing method thereof based on the kinetic information of spoilage microorganism growth of refrigerated food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 310053 building 10, No.1 Weiye Road, Binjiang District, Hangzhou City, Zhejiang Province Co-patentee after: Hangzhou Beingmate Infant and Mom Nutrition Co., Ltd. Patentee after: Bainmei Co., Ltd Co-patentee after: Heilongjiang Beingmate Diary Co., Ltd. Address before: 310051 No. 3758 South Ring Road, Hangzhou, Zhejiang, Binjiang District Co-patentee before: Hangzhou Beingmate Infant and Mom Nutrition Co., Ltd. Patentee before: Bei Yinmei Ying Tong food limited-liability company Co-patentee before: Heilongjiang Beingmate Diary Co., Ltd. |