CN103983626A - Reagent for detecting lipase and method for rapidly detecting lipase - Google Patents
Reagent for detecting lipase and method for rapidly detecting lipase Download PDFInfo
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- CN103983626A CN103983626A CN201410215727.1A CN201410215727A CN103983626A CN 103983626 A CN103983626 A CN 103983626A CN 201410215727 A CN201410215727 A CN 201410215727A CN 103983626 A CN103983626 A CN 103983626A
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- lipase
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- 108090001060 Lipase Proteins 0.000 title claims abstract description 25
- 102000004882 Lipase Human genes 0.000 title claims abstract description 25
- 239000004367 Lipase Substances 0.000 title claims abstract description 24
- 235000019421 lipase Nutrition 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 27
- 235000013365 dairy product Nutrition 0.000 claims abstract description 24
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 9
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 8
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 claims abstract description 8
- 239000007853 buffer solution Substances 0.000 claims abstract description 3
- 239000012530 fluid Substances 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 30
- 210000000481 breast Anatomy 0.000 claims description 13
- 238000013016 damping Methods 0.000 claims description 13
- 230000005284 excitation Effects 0.000 claims description 11
- 235000019626 lipase activity Nutrition 0.000 claims description 11
- 235000013336 milk Nutrition 0.000 claims description 10
- 239000008267 milk Substances 0.000 claims description 10
- 210000004080 milk Anatomy 0.000 claims description 10
- 235000020185 raw untreated milk Nutrition 0.000 claims description 10
- 238000002965 ELISA Methods 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000002421 anti-septic effect Effects 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- 235000020122 reconstituted milk Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 239000007974 sodium acetate buffer Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 238000006467 substitution reaction Methods 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 2
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- GRVDJDISBSALJP-UHFFFAOYSA-N methyloxidanyl Chemical group [O]C GRVDJDISBSALJP-UHFFFAOYSA-N 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- -1 methoxyl resorufin Chemical compound 0.000 abstract description 13
- 239000000243 solution Substances 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 2
- 239000003755 preservative agent Substances 0.000 abstract 1
- 230000002335 preservative effect Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 229910021642 ultra pure water Inorganic materials 0.000 description 5
- 239000012498 ultrapure water Substances 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241001494479 Pecora Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000020186 condensed milk Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000012859 sterile filling Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 108010072641 thermostable lipase Proteins 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
? | Blank | 1# | 2# | 3# |
0min | 140 | 152 | 132 | 124 |
40min | 199 | 587 | 516 | 340 |
Claims (7)
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CN201410215727.1A CN103983626B (en) | 2014-05-21 | 2014-05-21 | A kind of lipase quick determination method of lipase detection reagent |
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CN201410215727.1A CN103983626B (en) | 2014-05-21 | 2014-05-21 | A kind of lipase quick determination method of lipase detection reagent |
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CN103983626A true CN103983626A (en) | 2014-08-13 |
CN103983626B CN103983626B (en) | 2017-08-29 |
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CN201410215727.1A Active CN103983626B (en) | 2014-05-21 | 2014-05-21 | A kind of lipase quick determination method of lipase detection reagent |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104198474A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Detection kit for measuring content of lipase in serum by colorimetric method |
CN105651715A (en) * | 2016-01-25 | 2016-06-08 | 中国农业科学院农产品加工研究所 | Method and kit for high throughput testing of enzymatic activity in lipase in liquid milk product |
CN104730014B (en) * | 2015-03-27 | 2017-08-04 | 宁波博泰生物技术有限公司 | A kind of serum lipase determination kit and preparation method thereof |
CN113640262A (en) * | 2021-07-30 | 2021-11-12 | 安徽科技学院 | Technical method for rapidly identifying compost maturity degree by using fluorescence method |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR890003946B1 (en) * | 1985-05-03 | 1989-10-13 | 뵈링거 만하임 게엠배하 | Method for determination of lipase |
JPH09215500A (en) * | 1996-02-13 | 1997-08-19 | Towns:Kk | Substrate solution for lipase analysis and reagent solution kid for lipase analysis |
CN101131389A (en) * | 2006-08-22 | 2008-02-27 | 浙江养生堂天然药物研究所有限公司 | Method for screening pancreatic lipase restrainer from traditional Chinese medicine |
CN101324573A (en) * | 2007-06-12 | 2008-12-17 | 富士胶片株式会社 | Dry analytical element for lipase measurement |
CN102621138A (en) * | 2012-04-06 | 2012-08-01 | 上海蓝怡科技有限公司 | Preparation method of micro-emulsion kit |
-
2014
- 2014-05-21 CN CN201410215727.1A patent/CN103983626B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR890003946B1 (en) * | 1985-05-03 | 1989-10-13 | 뵈링거 만하임 게엠배하 | Method for determination of lipase |
JPH09215500A (en) * | 1996-02-13 | 1997-08-19 | Towns:Kk | Substrate solution for lipase analysis and reagent solution kid for lipase analysis |
CN101131389A (en) * | 2006-08-22 | 2008-02-27 | 浙江养生堂天然药物研究所有限公司 | Method for screening pancreatic lipase restrainer from traditional Chinese medicine |
CN101324573A (en) * | 2007-06-12 | 2008-12-17 | 富士胶片株式会社 | Dry analytical element for lipase measurement |
CN102621138A (en) * | 2012-04-06 | 2012-08-01 | 上海蓝怡科技有限公司 | Preparation method of micro-emulsion kit |
Non-Patent Citations (6)
Title |
---|
DEAN GILHAM ET AL.: "Techniques to measure lipase and esterase activity in vitro", 《METHODS》, 31 December 2005 (2005-12-31) * |
FARIHA HASAN ET AL.: "Methods for detection and characterization of lipases: A comprehensive review", 《BIOTECHNOLOGY ADVANCES》, 17 June 2009 (2009-06-17) * |
李宇辉等: "微生物脂肪酶的性质及应用研究现状", 《食品工业》 * |
沈瑛等: "单底物一步法测定血清脂肪酶及其临床应用", 《上海医学检验杂志》, vol. 16, no. 3, 31 December 2001 (2001-12-31) * |
郑毅等: "脂肪酶活力测定研究进展", 《工业微生物》, vol. 35, no. 4, 31 December 2005 (2005-12-31) * |
郭德军等: "原料乳中嗜冷菌及其主要热稳定性酶类的研究进展", 《中国乳品工业》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104198474A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Detection kit for measuring content of lipase in serum by colorimetric method |
CN104730014B (en) * | 2015-03-27 | 2017-08-04 | 宁波博泰生物技术有限公司 | A kind of serum lipase determination kit and preparation method thereof |
CN105651715A (en) * | 2016-01-25 | 2016-06-08 | 中国农业科学院农产品加工研究所 | Method and kit for high throughput testing of enzymatic activity in lipase in liquid milk product |
CN105651715B (en) * | 2016-01-25 | 2019-04-09 | 中国农业科学院农产品加工研究所 | A kind of high-throughput method and its kit for detecting lipase enzymatic activity in liquid diary product |
CN113640262A (en) * | 2021-07-30 | 2021-11-12 | 安徽科技学院 | Technical method for rapidly identifying compost maturity degree by using fluorescence method |
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Publication number | Publication date |
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CN103983626B (en) | 2017-08-29 |
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CB03 | Change of inventor or designer information |
Inventor after: Zhou Juanzuo Inventor after: Jin Jinghua Inventor after: Zhou Yue Inventor after: Wang Jiangyun Inventor after: Li Hongtuan Inventor before: Zhou Juanzuo |
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Effective date of registration: 20170427 Address after: 100085 room D304, zone D, level 12, Haidian District information road, Beijing, China Applicant after: Beijing peaceful Lv Yuan Science and Technology Ltd. Address before: 100085 Haidian District, Zhongguancun on the road to information on the ground floor, building 26, No. 1004, Applicant before: Zhou Juanzuo Applicant before: Jin Jinghua Applicant before: Zheng Peng Applicant before: Zhou Yue Applicant before: Wang Jiangyun |
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Effective date of registration: 20190517 Address after: 223201 A4-5 Floor, Huai'an Wisdom Valley, 19 Meigao Road, Huai'an Economic and Technological Development Zone, Jiangsu Province Patentee after: Huaian Anfu Technology Co. Ltd. Address before: Room D304, D District, 3rd Floor, 12 Shangdi Information Road, Haidian District, Beijing 100085 Patentee before: Beijing peaceful Lv Yuan Science and Technology Ltd. |
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