CN104031777A - Culture dish washing liquid and washing steps thereof - Google Patents
Culture dish washing liquid and washing steps thereof Download PDFInfo
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- CN104031777A CN104031777A CN201410260617.7A CN201410260617A CN104031777A CN 104031777 A CN104031777 A CN 104031777A CN 201410260617 A CN201410260617 A CN 201410260617A CN 104031777 A CN104031777 A CN 104031777A
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Abstract
The invention discloses culture dish washing liquid and washing steps thereof. The culture dish washing liquid comprises early washing liquid and late washing liquid, wherein the early washing liquid consists of the following components: 20g of para-sodium dodecyl benzene sulfonate, 30g of sodium sulfate, 7g of sodium carbonate, 25g of sodium silicate and 400ml of distilled water; the late washing liquid consists of the following components: 2g of oxalic acid, 100ml of 20% hydrochloric acid solution, 100ml of 60% sulfuric acid solution, 100ml of 25% nitric acid solution and 200ml of 60% ethanol. The washing liquid has the advantages of thorough cleaning, short cleaning time and no influence on the culture dish quality.
Description
Technical field
The present invention relates to a kind of culture dish washings and washing step thereof.
Background technology
Culture dish is a kind of labware for microorganism or cell cultures, is made up of the end and a lid of a flat circle plate-like.Culture dish material is divided into two classes substantially, is mainly plastic and glass, can also may the using for the adherent culture of vegetable material, microorganism culturing and zooblast of glass.Plastics may be polythene material, once property with nonexpondable, be applicable to the operation of laboratory inoculation, line, separation of bacterial, can be for the cultivation of vegetable material.Culture dish being fragile, frangible, the culture dish of finishing using preferably cleans up in time, leaves safe, fixing position in, prevents from damaging, breaking.The cleaning of culture dish generally through soaking, scrub, pickling and four steps of cleaning.Scrub with pickling process in all to use washings, the soil removability of washings is directly connected to the clean level of culture dish, affects reusing of culture dish.Existing culture dish washings cannot clean up culture dish even completely, and residual bacteria effect reuses to culture dish.
Summary of the invention
The technical problem to be solved in the present invention is to provide one and cleans up thoroughly, and scavenging period is short, does not affect the culture dish washings of culture dish quality.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: a kind of culture dish washings, and described culture dish washings in early stage is made up of by weight ratio following composition: 20g is to dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass, 400ml distilled water; Final wash liquid is made up of by weight ratio following composition: the salpeter solution that the hydrochloric acid soln of 2g oxalic acid, 100ml concentration 20%, the sulphuric acid soln of 100ml concentration 60%, 100ml concentration are 25%, the ethanol that 200ml concentration is 60%;
Another technical problem that the present invention will solve is to provide one and cleans up thoroughly, and scavenging period is short, does not affect the washing step of culture dish quality culture dish.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: comprise the following steps:
1) extract by weight ratio 20g dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass are dissolved in 400mL distilled water, obtain washings in early stage, for subsequent use;
2) extract by weight ratio in the mixed solution of the ethanol that hydrochloric acid soln that 2g oxalic acid is dissolved in 100ml concentration 20%, salpeter solution that 100ml concentration is 25% and 200ml concentration are 60%, again gained mixed solution is slowly poured into the sulphuric acid soln of 100ml concentration 60%, obtain final wash liquid, for subsequent use;
3) by used culture dish early stage washings soak half an hour, repeatedly scrub with banister brush; Do not stay dead angle, and prevent from destroying the smooth finish of vessel surface; The glassware scrubbing clean is cleaned, dried;
5) above-mentioned culture dish is dipped into 6-12 hour in final wash liquid, washings is static condition;
6) cleaning down culture dish, each culture dish at least repeatedly " water filling-turned letter " more than 15 times, finally embathes 2-3 time with redistilled water, dry or dry after package spare.
Embodiment
For the concrete function that can further understand feature of the present invention, technique means and reach, with embodiment, the present invention is described in detail below.
Embodiment:
A kind of culture dish washings, described culture dish washings in early stage is made up of by weight ratio following composition: 20g is to dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass, 400ml distilled water; Final wash liquid is made up of by weight ratio following composition: the salpeter solution that the hydrochloric acid soln of 2g oxalic acid, 100ml concentration 20%, the sulphuric acid soln of 100ml concentration 60%, 100ml concentration are 25%, the ethanol that 200ml concentration is 60%;
The washing step of described culture dish is as follows:
1) extract by weight ratio 20g dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass are dissolved in 400mL distilled water, obtain washings in early stage, for subsequent use;
2) extract by weight ratio in the mixed solution of the ethanol that hydrochloric acid soln that 2g oxalic acid is dissolved in 100ml concentration 20%, salpeter solution that 100ml concentration is 25% and 200ml concentration are 60%, again gained mixed solution is slowly poured into the sulphuric acid soln of 100ml concentration 60%, obtain final wash liquid, for subsequent use;
3) by used culture dish early stage washings soak half an hour, repeatedly scrub with banister brush; Do not stay dead angle, and prevent from destroying the smooth finish of vessel surface; The glassware scrubbing clean is cleaned, dried;
5) above-mentioned culture dish is dipped into 6-12 hour in final wash liquid, washings is static condition;
6) cleaning down culture dish, each culture dish at least repeatedly " water filling-turned letter " more than 15 times, finally embathes 2-3 time with redistilled water, dry or dry after package spare.
Experimental example:
Experimental subjects: 100 least recently used culture dish, are divided into two groups, every group of 50 people.
Experimental technique: control group cleans by general culture dish washings and step, experimental group is cleaned by culture dish washings of the present invention and step.Bacterium after record cleaning is residual respectively.
Experimental result 2:
From experimental result, general culture dish washings cannot thoroughly clean up culture dish, and residual bacterium is many, and uses this culture dish washings to clean up thoroughly, and scavenging period is short, does not affect culture dish quality.
Below the present invention is described in detail, the above, be only the present invention's preferred embodiment, when not limiting the scope of the present invention, allly does impartial variation and modification according to the application's scope, all should still belong in covering scope of the present invention.
Claims (2)
1. a culture dish washings, comprise washings and final wash liquid in earlier stage, it is characterized in that: described culture dish washings in early stage is made up of by weight ratio following composition: 20g is to dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass, 400ml distilled water; Final wash liquid is made up of by weight ratio following composition: the salpeter solution that the hydrochloric acid soln of 2g oxalic acid, 100ml concentration 20%, the sulphuric acid soln of 100ml concentration 60%, 100ml concentration are 25%, the ethanol that 200ml concentration is 60%.
2. a washing step for culture dish as claimed in claim 1, is characterized in that: comprise the following steps:
1) extract by weight ratio 20g dodecylbenzene sodium sulfonate, 30g sodium sulfate, 7g sodium carbonate, 25g water glass are dissolved in 400mL distilled water, obtain washings in early stage, for subsequent use;
2) extract by weight ratio in the mixed solution of the ethanol that hydrochloric acid soln that 2g oxalic acid is dissolved in 100ml concentration 20%, salpeter solution that 100ml concentration is 25% and 200ml concentration are 60%, again gained mixed solution is slowly poured into the sulphuric acid soln of 100ml concentration 60%, obtain final wash liquid, for subsequent use;
3) by used culture dish early stage washings soak half an hour, repeatedly scrub with banister brush; Do not stay dead angle, and prevent from destroying the smooth finish of vessel surface; The glassware scrubbing clean is cleaned, dried;
5) above-mentioned culture dish is dipped into 6-12 hour in final wash liquid, washings is static condition;
6) cleaning down culture dish, each culture dish at least repeatedly " water filling-turned letter " more than 15 times, finally embathes 2-3 time with redistilled water, dry or dry after package spare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410260617.7A CN104031777A (en) | 2014-06-12 | 2014-06-12 | Culture dish washing liquid and washing steps thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410260617.7A CN104031777A (en) | 2014-06-12 | 2014-06-12 | Culture dish washing liquid and washing steps thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN104031777A true CN104031777A (en) | 2014-09-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410260617.7A Pending CN104031777A (en) | 2014-06-12 | 2014-06-12 | Culture dish washing liquid and washing steps thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418764A (en) * | 2017-06-20 | 2017-12-01 | 青岛金典生化器材有限公司 | A kind of glass culture dish cleaning solution and its washing methods |
| CN109442869A (en) * | 2018-09-06 | 2019-03-08 | 上海航天精密机械研究所 | Spherical tank drying means |
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| CN101451092A (en) * | 2007-12-05 | 2009-06-10 | 鲁习武 | High efficiency universal cleaning agent |
| JP2010229035A (en) * | 2007-08-01 | 2010-10-14 | Taisho Pharmaceutical Co Ltd | Pyridopyrimidin-4-one derivative |
| CN103087858A (en) * | 2013-02-06 | 2013-05-08 | 厦门富可汽车配件有限公司 | Solid glass cleaner and processing method thereof |
| CN103320241A (en) * | 2012-03-21 | 2013-09-25 | 上海盈致橡塑制品有限公司 | Detergent for plastic products |
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2014
- 2014-06-12 CN CN201410260617.7A patent/CN104031777A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2010229035A (en) * | 2007-08-01 | 2010-10-14 | Taisho Pharmaceutical Co Ltd | Pyridopyrimidin-4-one derivative |
| CN101451092A (en) * | 2007-12-05 | 2009-06-10 | 鲁习武 | High efficiency universal cleaning agent |
| CN103320241A (en) * | 2012-03-21 | 2013-09-25 | 上海盈致橡塑制品有限公司 | Detergent for plastic products |
| CN103087858A (en) * | 2013-02-06 | 2013-05-08 | 厦门富可汽车配件有限公司 | Solid glass cleaner and processing method thereof |
Non-Patent Citations (3)
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| 吕冬霞主编: "《细胞生物学技术》", 31 March 2010, 科学出版社 * |
| 李云巧编著: "《实验室溶液制备手册》", 31 January 2006, 化学工业出版社 * |
| 白金泉等编: "《表面活性剂在洗涤工业中的应用》", 31 July 2003, 化学工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418764A (en) * | 2017-06-20 | 2017-12-01 | 青岛金典生化器材有限公司 | A kind of glass culture dish cleaning solution and its washing methods |
| CN109442869A (en) * | 2018-09-06 | 2019-03-08 | 上海航天精密机械研究所 | Spherical tank drying means |
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20140910 |