CN104027479B - A kind of ganoderma tea polyphenol composite antioxidant - Google Patents
A kind of ganoderma tea polyphenol composite antioxidant Download PDFInfo
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- CN104027479B CN104027479B CN201410225073.0A CN201410225073A CN104027479B CN 104027479 B CN104027479 B CN 104027479B CN 201410225073 A CN201410225073 A CN 201410225073A CN 104027479 B CN104027479 B CN 104027479B
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Abstract
The present invention provides a kind of ganoderma tea polyphenol composite antioxidant, using different material antioxidation approach and the difference of mode, synergy is compounded between raw material, there is provided a kind of composite antioxidant, including Tea Polyphenols, Ganodenna Lucidum P.E and agrocybe extract, content is respectively:Tea Polyphenols 15 70%, Ganodenna Lucidum P.E 5 40%, agrocybe extract 5 50%.By measure and calculation, the Berenbaum indexes of inventive formulation are less than having collaboration antioxidation between different component in 1, i.e. inventive formulation.And it is common edible natural products that the present invention is raw materials used, wide material sources have no toxic side effect.
Description
【Technical field】
The invention belongs to medicines and health protection field, it is related to a kind of ganoderma lucidum and the compound antioxidant of Tea Polyphenols.
【Background technology】
During human senility, oxidation resistance constantly declines, and it is exactly the mistake for producing free radical in vivo to aoxidize
Journey, research finds that Tea Polyphenols and Ganodenna Lucidum P.E have the ability for preferably removing free radical.Ganoderma lucidum is a kind of widely used
Integration of drinking and medicinal herbs fungi, its principle active component is GL-B, and GL-B is produced in can catching lipid peroxidation chain reaction
Raw active oxygen, reduces the length of peroxidatic reaction of lipid chain, blocks or slow down the carrying out of lipid peroxidation.Plant polyphenol is one
Class polyol, is a kind of natural nontoxic antioxidant, and on the one hand Tea Polyphenols can be reduced certainly by suppressing oxidizing ferment
By the generation of base, to improve its antioxidation;On the other hand by inactivating free radical, antioxidase is protected, improves anti-in vivo
The activity and then enhancing antioxidation of oxidizing ferment.In order to obtain preferably remove free radical effect, the present invention by Tea Polyphenols,
Ganodenna Lucidum P.E and agrocybe extract science are assembled, and develop a kind of compound anti-oxidation product.
【The content of the invention】
The present invention utilizes the compounding synergy between the approach of different material antioxidation and the difference of mode, raw material,
A kind of composite antioxidant is developed, it is measured and is removed fat peroxy radical, hydroxy radical and ultra-oxygen anion free radical
Berenbaum indexes be respectively less than 1 (Berenbaum indexes be the evaluations that were proposed in 1985 of Berenbaum two or more
The standard of collaboration or antagonism between medicine,Wherein, Xi:I-th medicine is in joint
Dosage during medication, Xie:I-th medicine can produce the dosage with drug combination same effect, n when being used alone:Joint is used
The medicine number of medicine.When Berenbaum indexes are less than 1, medication combined is synergy, and works as Berenbaum indexes more than 1
When, then prompting has antagonism), i.e., there is synergy in the present invention between different component.The present invention takes following technical side
Case:
A kind of ganoderma tea polyphenol composite antioxidant, including Tea Polyphenols, Ganodenna Lucidum P.E and agrocybe extract, the tea
The percentage that polyphenol, Ganodenna Lucidum P.E and agrocybe extract account for composite antioxidant is respectively:Tea Polyphenols 15-70%, ganoderma lucidum
Extract 5-40%, agrocybe extract 5-50%.
The Tea Polyphenols is green-tea extract;Thick many candies content is 8-15% in the Ganodenna Lucidum P.E;The agrocybe
Thick many candies content is 2.5-6% in extract.
Preferably, above-mentioned ganoderma tea polyphenol composite antioxidant also includes auxiliary material, and the auxiliary material includes microcrystalline cellulose, hard
Fatty acid magnesium, maltodextrin and silica.
Preferably, the Tea Polyphenols, Ganodenna Lucidum P.E and agrocybe extract account for the percentage difference of composite antioxidant
For:Tea Polyphenols 15%, Ganodenna Lucidum P.E 15%, agrocybe extract 43%.
Preferably, the auxiliary material content is respectively microcrystalline cellulose 17%, magnesium stearate 1%, maltodextrin 8%, dioxy
SiClx 1%.
Preferably, the ganoderma tea polyphenol composite antioxidant is a kind of tablet, and its manufacture craft is:Dispensing → premix →
Powder vertical compression → plain piece → film coating, the piece that dries in the air → Film coated tablets to be packaged.
Preferably, the agrocybe extract is obtained by the following method:With water as extract solution, extract at twice,
First time extraction conditions are:100 DEG C of Extracting temperature, is incubated 2h, solid-liquid ratio=1:12;Second extraction conditions be:Extracting temperature
100 DEG C, it is incubated 1h, solid-liquid ratio=1:8, by twice extract solution merge after 70-80 DEG C, vacuum -0.04Mpa -
Pol is concentrated under conditions of 0.07Mpa for 3.5-4.0, spray drying.
The Ganodenna Lucidum P.E is obtained by the following method:With water as extract solution, extract at twice, extract for the first time
Condition is:100 DEG C of Extracting temperature, is incubated 2h, solid-liquid ratio=1:12;Second extraction conditions be:100 DEG C of Extracting temperature, insulation
1h, solid-liquid ratio=1:8, after extract solution twice is merged under conditions of 70-80 DEG C, vacuum -0.04Mpa -0.07Mpa
Pol is concentrated into for 3.5-4.0, spray drying.
Advantage and have the beneficial effect that this reality is invented:(1) present invention utilizes Tea Polyphenols, Ganodenna Lucidum P.E and tea tree first
Mushroom extract, is made composite antioxidant, improves the anti-oxidant efficiency of single raw material, reduces the dosage of medication.(2) present invention matches somebody with somebody
Major ingredient used is common edible natural products in side, wide material sources and has no toxic side effect.
【Specific embodiment】
With reference to specific embodiment, the invention will be further described, but the present invention includes scope not limited to this.
Embodiment one
(1) agrocybe extract is prepared
With water as extract solution, extract at twice, first time extraction conditions are:100 DEG C of Extracting temperature, is incubated 2h, solid-liquid ratio
=1:12;Second extraction conditions be:100 DEG C of Extracting temperature, is incubated 1h, solid-liquid ratio=1:8, after extract solution twice is merged
Pol is concentrated under conditions of 70-80 DEG C, vacuum -0.04Mpa -0.07Mpa for 3.5-4.0, spray drying obtains tea
Tree mushroom water extract.
(2) Ganodenna Lucidum P.E is prepared
With water as extract solution, extract at twice, first time extraction conditions are:100 DEG C of Extracting temperature, is incubated 2h, solid-liquid ratio
=1:12;Second extraction conditions be:100 DEG C of Extracting temperature, is incubated 1h, solid-liquid ratio=1:8, after extract solution twice is merged
Pol is concentrated under conditions of 70-80 DEG C, vacuum -0.04Mpa -0.07Mpa for 3.5-4.0, spray drying is obtained spirit
Sesame water extract.
(3) composite antioxidant is prepared
Weigh Tea Polyphenols 20kg (buying in Shanxi Jiahe Plant Chemical Co., Ltd.), Ganodenna Lucidum P.E 40kg, tea
Tree mushroom extract 13kg, maltodextrin 8kg, microcrystalline cellulose 17kg, coated tablet is made according to the common process of tablet, is designated as
Formula 1.
(4) measure of fat peroxy radical (ROO) Scavenging activity
By 5mL ethanol, 5mL0.1mol/L phosphate buffers (pH8.0), 0.1mL linoleic acid respectively with 5mg/mL, 6mg/
Sample (volume of sample is 1mL) mixing of mL, 7mg/mL, 8mg/mL, 9mg/mL, 10mg/mL, uses 50W ultra violet lamps
45min.It is subsequently adding 4mL trichloroacetic acids (20%, W/W), 1mL thiobarbituricacidα-s (3%), 95 DEG C of reaction 90min, ice bath, 4
DEG C centrifugation, with ultraviolet-visible spectrophotometer in 532nm at colorimetric, survey its absorbance A j, with distilled water as blank, survey
Obtain each sample as shown in table 1 to the clearance rate of fat peroxy radical.Wherein, the computing formula of free radical scavenging activity is:P=(Ao-
Aj)/Ao, Ao are the absorbance of blank.
The clearance rate P (%) of the fat peroxy radical of table 1
Calculate the IC that said sample is drawn after analyzing50Value is as shown in table 2:
The IC of the removing fat peroxy radical of each sample of table 250Value
With reference to the computational methods that Berenbaum was proposed in 1985:Wherein, Xi:
Dosage of i-th medicine in drug combination, Xie:I-th medicine can be produced and drug combination same effect when being used alone
Dosage.n:The medicine number of drug combination.When Berenbaum indexes are less than 1, medication combined is synergy, and is worked as
When Berenbaum indexes are more than 1, then antagonism is pointed out.Can calculate:Berenbaum indexes are 0.8438.Illustrate formula
There is collaboration antioxidation between each component in 1.
Embodiment two
The preparation process of agrocybe and ganoderma lucidum water extract is with embodiment one.
Weigh Tea Polyphenols 70kg (buying in Shanxi Jiahe Plant Chemical Co., Ltd.), Ganodenna Lucidum P.E 5kg, tea tree
Mushroom extract 5kg, maltodextrin 5kg, microcrystalline cellulose 12kg, coated tablet is made according to the common process of tablet, is designated as matching somebody with somebody
Side 2.
The measure of hydroxyl radical free radical clearance rate:
Be separately added into 10mL colorimetric cylinders 0.05% bromocresol purple solution 0.4mL, 0.1mol/L hydrochloric acid 0.5mL,
1mmol/L green-vitriol solution 1.0mL, scale is diluted to redistilled water, is shaken up, and is placed in 30 DEG C of waters bath with thermostatic control and is reacted
8min, is rapidly added 0.05mol/L NaF solution 0.2mL after taking-up, mix terminating reaction.1cm cuvettes are used, is with distilled water
Reference, the mensuration absorbance at 420nm determines 3 Ao that average.
Ibid, 0.1mol/L hydrogen peroxidase 10 .5mL are added after adding 1mmol/L green-vitriol solution, solution is determined
Absorbance, determines 3 A that average, and calculate absorbance decreasing value (Ao-A).
Prior to be separately added into 10mL colorimetric cylinders 0.05% bromocresol purple solution 0.4mL, 0.1mol/L hydrochloric acid 0.5mL,
1mmol/L green-vitriol solution 1.0mL, 0.1mol/L hydrogen peroxidase 10 .5mL, then the sample for being separately added into 10mg/ml
0.06mL, 0.07mL, 0.08mL, 0.09mL, 0.10mL, scale is diluted to redistilled water, is shaken up, and body is determined according to preceding method
It is absorbance A s.Clearance rate computing formula is:D=[(As-A)/(Ao-A)] × 100%, its result is as shown in table 3:
The clearance rate D (%) of the hydroxyl radical free radical of table 3
Calculate the IC that said sample is drawn after analyzing50Value is as shown in table 4:
The IC of each sample scavenging hydroxyl of table 450Value
With reference to the computational methods that Berenbaum was proposed in 1985, it is 0.8703, explanation to calculate Berenbaum indexes
There is collaboration antioxidation in formula 2 between each component.
Embodiment three
The preparation process of agrocybe and ganoderma lucidum water extract is with embodiment one.
Weigh Tea Polyphenols 15kg (buying in Shanxi Jiahe Plant Chemical Co., Ltd.), Ganodenna Lucidum P.E 15kg, tea
Tree mushroom extract 43kg, maltodextrin 8kg, microcrystalline cellulose 17kg, coated tablet is made according to the common process of tablet, is designated as
Formula 3.
The measure of ultra-oxygen anion free radical clearance rate:
Using pyrogallol 325nm micromethods, mouse thymus cells speed is measured for 0.004OD/S.First in test tube
Add pH8.2 50mmol/L Tris-HCl buffer solution 4.5mL, be then respectively adding 0.1mL, 0.2mL, 0.3mL, 0.4mL,
The sample (sample solution concentration is 10mg/mL) of 0.5mL, adds appropriate distilled water, overall solution is 5mL.In preservation at 25 DEG C
20min, is added in the pyrogallol 0.01mL preheated in 25 DEG C immediately after taking-up, shake up pour into cuvette rapidly, every 30s
An absorbance is determined, autoxidation speed S is calculated.With 10mmol/L HCl as blank.Clearance rate computing formula is:E=
(0.004-S)/0.004 × 100%, as a result as shown in table 5:
The clearance rate (%) of the ultra-oxygen anion free radical of table 5
Calculate the IC that said sample is drawn after analyzing50Value is as shown in table 6:
Each sample of table 6 removes the IC of superoxide anion50Value
With reference to the computational methods that Berenbaum was proposed in 1985, it can be deduced that Berenbaum indexes are 0.744, explanation
There is collaboration antioxidation in formula 3 between each component.
Example IV
The preparation process of agrocybe and ganoderma lucidum water extract is with embodiment one.
Weigh Tea Polyphenols 15kg (buying in Shanxi Jiahe Plant Chemical Co., Ltd.), Ganodenna Lucidum P.E 15kg, tea
Tree mushroom extract 50kg, maltodextrin 5kg, microcrystalline cellulose 12kg, coated tablet is made according to the common process of tablet, i.e. spirit
Sesame Tea Polyphenols composite antioxidant.
Claims (8)
1. a kind of ganoderma tea polyphenol composite antioxidant, it is characterised in that by Tea Polyphenols, Ganodenna Lucidum P.E, agrocybe extract
Constituted with auxiliary material, wherein, the content of the Tea Polyphenols, Ganodenna Lucidum P.E and agrocybe extract is respectively:Tea Polyphenols 15-
70%, Ganodenna Lucidum P.E 5-40%, agrocybe extract 5-50%.
2. ganoderma tea polyphenol composite antioxidant according to claim 1, it is characterised in that the Tea Polyphenols is carried for green tea
Take thing;Thick many candies content is 8-15% in the Ganodenna Lucidum P.E;Thick many candies content is 2.5- in the agrocybe extract
6%.
3. ganoderma tea polyphenol composite antioxidant according to claim 2, it is characterised in that the auxiliary material is by microcrystalline cellulose
Element, magnesium stearate, maltodextrin and silica composition.
4. ganoderma tea polyphenol composite antioxidant according to claim 3, it is characterised in that the auxiliary material content is respectively
Microcrystalline cellulose 17%, magnesium stearate 1%, maltodextrin 8%, silica 1 %.
5. ganoderma tea polyphenol composite antioxidant according to claim 4, it is characterised in that the Tea Polyphenols, ganoderma lucidum are extracted
The percentage composition that thing and agrocybe extract account for composite antioxidant is respectively:Tea Polyphenols 15%, Ganodenna Lucidum P.E 15%, tea
Tree mushroom extract 43%.
6. ganoderma tea polyphenol composite antioxidant according to claim 4, it is characterised in that the ganoderma lucidum Tea Polyphenols is combined
Antioxidant is a kind of tablet, is made up of following technique:Dispensing → premix → powder vertical compression → plain piece → film coating,
Dry in the air piece → Film coated tablets to be packaged.
7. the ganoderma tea polyphenol composite antioxidant according to any one of claim 1-5, it is characterised in that the ganoderma lucidum
Extract is obtained by the following method:With water as extract solution, extract at twice, first time extraction conditions are:Extracting temperature
100 DEG C, it is incubated 2h, solid-liquid ratio=1:12;Second extraction conditions be:100 DEG C of Extracting temperature, is incubated 1h, solid-liquid ratio=1:8,
Pol is concentrated into after extract solution twice is merged under conditions of 70-80 DEG C, vacuum -0.04Mpa -0.07Mpa for 3.5-
4.0, spray drying.
8. the ganoderma tea polyphenol composite antioxidant according to any one of claim 1-5, it is characterised in that the tea tree
Mushroom extract is obtained by the following method:With water as extract solution, extract at twice, first time extraction conditions are:Extract temperature
100 DEG C of degree, is incubated 2h, solid-liquid ratio=1:12;Second extraction conditions be:100 DEG C of Extracting temperature, is incubated 1h, solid-liquid ratio=1:
8, being concentrated into pol under conditions of 70-80 DEG C, vacuum -0.04Mpa -0.07Mpa after extract solution twice is merged is
3.5-4.0, spray drying.
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