CN104026047A - Zero-damage injection method of immune reaction allogenes of veneridae shellfish - Google Patents
Zero-damage injection method of immune reaction allogenes of veneridae shellfish Download PDFInfo
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- CN104026047A CN104026047A CN201410202494.1A CN201410202494A CN104026047A CN 104026047 A CN104026047 A CN 104026047A CN 201410202494 A CN201410202494 A CN 201410202494A CN 104026047 A CN104026047 A CN 104026047A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a zero-damage injection method of immune reaction allogenes of a veneridae shellfish. The method comprises the following steps that an injector with a syringe needle is prepared, a pathogen is sucked by the injector, the veneridae shellfish to be injected is prepared, an intersection point of the tail end of the outer ligament and the edge of the shell of the veneridae shellfish is found out, the intersection point serves as a collection point, the veneridae shellfish to be injected is stably held by one hand, the point of the syringe needle of the injector is aligned with the collection point by the other hand, the included angle between the point of the syringe needle and the tangent line of the edge of the shell is made to range from 30 degrees to 60 degrees, the point of the syringe needle is sent to the soft portion in the veneridae shellfish to be identified through the collection point, and the pathogen is injected into the veneridae shellfish. The injection method can be used for conducting a shellfish infection immunity test by injecting the pathogen into the shellfish on the premise that the shell of the shellfish is not damaged.
Description
Technical field
The invention belongs to marine biotechnology field, relate in particular to a kind of not damaged input method of Veneridae shellfish immune response allogene.
Background technology
In the shellfish of China's cultivation, Veneridae shellfish is in occupation of important cultivation status.For example, the young Ruditapes philippinarum belonging to of Veneridae clam is one of China's four large cultivated shellfishes, has very high economic worth.On market, common purple Saxidomus, clam, equilateral shallow clam, thin slice mirror clam all belongs to Veneridae shellfish simultaneously.From Veneridae shellfish, as important edible shellfish, researchers have just carried out much research to shellfish.And in recent years due to the destruction gradually of environment, in body of shellfish, there are various causal organisms, as, the handkerchief gold worm in Ruditapes philippinarum etc., the generation of these causal organisms can directly have influence on its economic worth.Researchers have come to realise the importance of research pathogen on shellfish impact, and then have carried out the para-infectious immunity test of shellfish.
At present, in the test of shellfish infection immunity, in order to inject various pathogens (bacterium, fungi, virus etc.) in shellfish, what generally take is that the mode of manually prizing shell or punching is carried out infection experiment, this method often needs special apparatus or equipment, and the amount of labour is also relatively large, the input method of this damage type tends to cause shellfish to produce stress reaction simultaneously, and then brings larger error to test.
Summary of the invention
The present invention is in order to solve the existing above-mentioned deficiency of prior art, proposes a kind ofly can pathogen be input to shellfish inside under the prerequisite of shellfish housing not being destroyed to carry out the input method of shellfish infection immunity test.
Technical solution of the present invention is: a kind of not damaged input method of Veneridae shellfish immune response allogene, is characterized in that: described method is carried out according to following steps:
A, prepare with the syringe of syringe needle, and draw pathogen with syringe, prepare Veneridae shellfish to be entered,
B, the external ligament end that finds Veneridae shellfish and the plotted point of shell edge, this point is designated as collection point,
C, one hand hold steady Veneridae shellfish to be entered, with another hand by the tip alignment collection point of syringe, allow needle point and shell edge tangent line be 30-60 degree angle, needle point is sent into soft body in Veneridae shellfish to be identified by collection point, to the inner input of Veneridae shellfish pathogen.
Syringe in described a step can be disposable sterilized injector, can be also sterile-processed syringe.
Compared with the existing technology, tool has the following advantages in the present invention:
Allogene input method disclosed in this invention, can be under the prerequisite of shellfish housing not being destroyed, pathogen is input to shellfish inside, the method of shell being pried open or being holed than traditional employing mechanical type instrument, apparatus is simple and easy to get, easy to operation, the more important thing is, because the method can not cause any type of destruction to shell, therefore can not affect experimental result because external force overstimulation produces stress reaction, damage littlely, error is little, for good scientific research basis has been established in the test of shellfish infection immunity.Therefore can say that it has possessed multiple advantage, be particularly suitable for applying in the art, its market prospects are very wide, have the significance of scientific research of outbalance.
Brief description of the drawings
Fig. 1 is Veneridae shellfish Ruditapes philippinarum not damaged injection embodiment plane of structure figure and vertical face figure.
Fig. 2 is Veneridae shellfish thin slice mirror clam not damaged injection embodiment plane of structure figure and vertical face figure.
Embodiment
Below in conjunction with brief description of the drawings the specific embodiment of the present invention.
A kind of not damaged input method of Veneridae shellfish immune response allogene, carry out according to following steps: first prepare the syringe with syringe needle, the syringe here can be disposable sterilized injector, also can adopt the reusable syringe through disinfecting, and draw pathogen with syringe; Then prepare Veneridae shellfish to be entered, and find the external ligament end of this shell and the plotted point of shell edge, this point is designated as to collection point; One hand holds steady Veneridae shellfish to be entered, with another hand by the tip alignment collection point of syringe, allow needle point and shell edge tangent line be 30-60 degree angle, needle point is sent into soft body in Veneridae shellfish to be identified by collection point, to the inner input of Veneridae shellfish pathogen.Can normally survive through this input method Veneridae shellfish after treatment, and 100% all can there is immune response.
embodiment 1
In January, 2014, in Liaoning Province's shellfish stock breeding Engineering Technical Research Centre, disclose method with the present invention Ruditapes philippinarum has been carried out to infection immunity test.As shown in Figure 1.
Pathogen is: peptide glycan (PGN), β whole glucan particles (WGP), outer membrane protein lipopolysaccharides (LPS) or viral double-stranded RNA synthetic (PIC), and concentration is 100 μ g/mL; First prepare disposable sterilized injector, draw pathogen solution, determine the external ligament end of Ruditapes philippinarum and the plotted point at shell edge, the input point that this point is real-time operation; Left hand holds steady Ruditapes philippinarum, the right hand is aimed at this point with syringe needle point, adjust needle point and shell edge tangential angle and be 35 degree, needle point is sent into the soft body of Ruditapes philippinarum, and input pathogen (one in PGN, WGP, LPS, PIC) in Ruditapes philippinarum, after operation, Ruditapes philippinarum can normally be survived, and 100% immune response occurs.
Wherein, 150 individual Ruditapes philippinarums are carried out to input experiment, found all to have occurred immune response, through 15d test, found without dead individual survival rate 100%.
embodiment 2
In February, 2014, in Liaoning Province's shellfish stock breeding Engineering Technical Research Centre, disclose method thin slice mirror clam with the present invention and carried out infection immunity test.As shown in Figure 2.
Pathogen is: peptide glycan (PGN), β whole glucan particles (WGP), outer membrane protein lipopolysaccharides (LPS) or viral double-stranded RNA synthetic (PIC), and concentration is 100 μ g/mL; First prepare disposable sterilized injector, draw pathogen solution, determine the external ligament end of thin slice mirror clam and the plotted point at shell edge, the input point that this point is real-time operation; Left hand holds steady thin slice mirror clam, the right hand is aimed at this point with syringe needle point, adjust needle point and shell edge tangential angle and be 55 degree, needle point is sent into the soft body of thin slice mirror clam, and input pathogen (one in PGN, WGP, LPS, PIC) in thin slice mirror clam, after operation, Ruditapes philippinarum can normally be survived, and 100% immune response occurs.
Wherein, 100 individual thin slice mirror clams are carried out to input experiment, found all to have occurred immune response, through 40d test, found without dead individual survival rate 100%.
Claims (2)
1. a not damaged input method for Veneridae shellfish immune response allogene, is characterized in that: described method is carried out according to following steps:
Prepare the syringe with syringe needle, and draw pathogen with syringe, prepare Veneridae shellfish to be entered,
Find the external ligament end of Veneridae shellfish and the plotted point of shell edge, this point is designated as collection point,
One hand holds steady Veneridae shellfish to be entered, with another hand by the tip alignment collection point of syringe, allow needle point and shell edge tangent line be 30-60 degree angle, needle point is sent into soft body in Veneridae shellfish to be identified by collection point, to the inner input of Veneridae shellfish pathogen.
2. the not damaged input method of a kind of Veneridae shellfish immune response allogene according to claim 1, is characterized in that: the syringe in described a step can be disposable sterilized injector, can be also sterile-processed syringe.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410202494.1A CN104026047A (en) | 2014-05-14 | 2014-05-14 | Zero-damage injection method of immune reaction allogenes of veneridae shellfish |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410202494.1A CN104026047A (en) | 2014-05-14 | 2014-05-14 | Zero-damage injection method of immune reaction allogenes of veneridae shellfish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931468A (en) * | 2018-05-16 | 2018-12-04 | 中国海洋大学 | A kind of method of easy quickly detection oyster ploidy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11196706A (en) * | 1998-01-07 | 1999-07-27 | Kajima Corp | Labeling shellfish to be labeled |
| CN102461471A (en) * | 2010-11-11 | 2012-05-23 | 湖南文理学院 | Culture method of amorphous pearls |
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2014
- 2014-05-14 CN CN201410202494.1A patent/CN104026047A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11196706A (en) * | 1998-01-07 | 1999-07-27 | Kajima Corp | Labeling shellfish to be labeled |
| CN102461471A (en) * | 2010-11-11 | 2012-05-23 | 湖南文理学院 | Culture method of amorphous pearls |
Non-Patent Citations (4)
| Title |
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| 刘可君等: "鳗弧菌侵染对青蛤溶菌酶和超氧化物岐化酶活性的影响", 《四川动物》 * |
| 宋欣等: "鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)磷酸酶活性的影响", 《海洋与湖沼》 * |
| 樊甄娇等: "鳗弧菌注射对栉孔扇贝免疫活性的影响", 《南方水产》 * |
| 潘宝平等: "青蛤(Cyclina sinensis)溶菌酶基因在鳗弧菌(Vibrio anguillarum)刺激下的表达", 《海洋与湖沼》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931468A (en) * | 2018-05-16 | 2018-12-04 | 中国海洋大学 | A kind of method of easy quickly detection oyster ploidy |
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