CN104013575B - A kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation and preparation method thereof - Google Patents
A kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation and preparation method thereof Download PDFInfo
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- CN104013575B CN104013575B CN201410282237.3A CN201410282237A CN104013575B CN 104013575 B CN104013575 B CN 104013575B CN 201410282237 A CN201410282237 A CN 201410282237A CN 104013575 B CN104013575 B CN 104013575B
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Abstract
The present invention provides a kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation and preparation method thereof, it is characterized in that, including step: S1, astaxanthin oil is mixed to form with oil phase first mixes oil phase, and the weight of this astaxanthin oil accounts for less than the 40% of this first mixing oil phase weight；S2, being added to surfactant mix homogeneously at this first miscella and form the second mixing oil phase, this surfactant is 2:3 with this first weight ratio mixing oil phase；S3, this second mixing oil phase in add concentration less than 0.2% hyaluronic acid aqueous solution, stir, wherein this hyaluronic acid aqueous solution accounts for the 90% of gross weight.Astaxanthin nano-emulsion preparation that the present invention provides and preparation method thereof, without high pressure equipment, the method utilizing mental retardation nano-emulsion, astaxanthin is embedded in nano-emulsion, improve the stability of astaxanthin, the use of acetone and other organic solvent can be avoided, be simultaneously introduced hyaluronic acid aqueous solution and improve the speed through epidermis of astaxanthin nano-emulsion and improve the heat stability of astaxanthin in nano-emulsion.
Description
Technical field
The present invention relates to the preparation method of the nano-emulsion carrier for lyophobic dust astaxanthin, particularly relate to a kind of transparent
Matter acid functionalization astaxanthin nano-emulsion preparation and preparation method thereof.
Background technology
Astaxanthin (Astaxanthin) is keto-acid carotenoid, and its molecular formula is C40H52O4, relative molecular weight is
596. 86.The oxidation resistance of astaxanthin is strong, and for 550 times, 10 times of beta-carotene of vitamin e, and astaxanthin is a lot
Product is all proved to be safely and effectively, therefore at medicine, and food, cosmetics there are the most wide market and huge development dive
Power.
Astaxanthin is hydrophobic material, is easily oxidized in the environment, does not tolerates High Temperature High Pressure, so astaxanthin
Application is restricted.In prior art when preparing relevant astaxanthin carrier, typically astaxanthin oil being dissolved in acetone etc. has
In machine solvent, and using high pressure homogenizer or Ultrasonic Cell Disruptor to input energy from the external world, the unstability of astaxanthin itself is made
Become it to lose under violent working condition, reduce the bioavailability of astaxanthin.
If not using the said equipment, can directly chelate astaxanthin or astaxanthin is direct with biomacromolecules such as cyclodextrin
It is dissolved in the organic solvent can applied in food or health product, such as ethanol.Use biomacromolecule chelates, right
The protective effect of astaxanthin is not very well, because the porous of structure of biological macromolecule；Secondly biomacromolecule was storing
In journey, instability, reduces the shelf life of product.Astaxanthin is dissolved with ethanol, owing to ethanol is easy in food or health product
Volatilization, can cause preparation unstable, and the protective effect to astaxanthin is not the strongest.
Being wrapped in by astaxanthin in nano-emulsion carrier, the dilution stability of emulsion is also a weight of nano-emulsion support
Want measurement index, nano-emulsion compare microemulsion advantage for dilute certain multiple time, it is possible to keep the integrity of this body structure,
But dilution stability is also relative, in actual applications, when extension rate increases, and surfactant concentration is less than nano-emulsion
During the critical aggregation concentration of balling-up, nano-emulsion is assembled, and produces precipitation.
It addition, nano-emulsion field of medicaments as the carrier of topical, at cosmetic field as the load of active substance
During body, the speed through epidermis is its another important indicator.When using nano-emulsion as astaxanthin carrier, if nano-emulsion
Epidermis can not be passed through rapidly, can deposit at skin surface, especially when surfactant concentration is bigger, easily cause skin
Allergy.
Summary of the invention
The features and advantages of the present invention are partly stated, or can from this description it is clear that or
Person can learn by putting into practice the present invention.
For solve lyophobic dust astaxanthin application problem, improve the stability of astaxanthin, astaxanthin nano-emulsion saturating
Crossing speed and the dilution stability of astaxanthin nano-emulsion of epidermis, the present invention provides a kind of hyaluronic acid functionalization astaxanthin nanometer
The preparation method of emulsion formulation, it is characterised in that include step: S1, astaxanthin oil is mixed to form with oil phase first mixes oil phase,
And less than 40% that the weight of this astaxanthin oil is this first mixing oil phase weight；S2, this first miscella be added to surface live
Property agent and mix homogeneously formed second mixing oil phase, this surfactant is 2:3 with this first weight ratio mixing oil phase；S3、
In this second mixing oil phase, add the concentration hyaluronic acid aqueous solution less than 0.2%, stir, wherein this hyalomitome sour water
The weight of solution accounts for the 90% of gross weight.
Preferably, in this step S1, in this astaxanthin oil, content astaxanthin is 5%.
Preferably, in this step S1, the weight of this astaxanthin oil is the 2% of this first mixing oil phase weight.
Preferably, in this step S3, when adding hyaluronic acid aqueous solution in this second mixing oil phase, this hyaluronic acid
Aqueous solution is added dropwise over.
Preferably, the solvent in this hyaluronic acid aqueous solution is distilled water.
Preferably, in this step S3, magnetic agitation is used will this second mixing oil phase to stir with hyaluronic acid aqueous solution
Mix uniformly.
The present invention also provides for a kind of hyaluronic acid functionalization astaxanthin nano-emulsion system, it is characterised in that be the first miscella
The emulsion oil-in-water that phase, surfactant, the concentration hyaluronic acid aqueous solution less than 0.2% is formed, wherein, this hyaluronic acid
The weight of aqueous solution accounts for the 90% of gross weight, and this first mixing oil phase, the weight ratio of surfactant are 3:2；This first miscella
Include astaxanthin oil and oil phase, and less than 40% that the weight of this astaxanthin oil is this first mixing oil phase weight mutually.
Preferably, in this astaxanthin oil, content astaxanthin is 5%.
Preferably, the solvent in this hyaluronic acid aqueous solution is distilled water.
Preferably, the weight of this astaxanthin oil is the 2% of this first mixing oil phase weight.
By reading description, those of ordinary skill in the art will be best understood the feature of these technical schemes and interior
Hold.
Accompanying drawing explanation
Below with reference to accompanying drawing and combine example be specifically described the present invention, advantages of the present invention and implementation will
Becoming apparent from, wherein content shown in accompanying drawing is only used for explanation of the present invention, and does not constitute to the present invention in all senses
On restriction, in the accompanying drawings:
Fig. 1 is the flow process signal of the preparation method of the hyaluronic acid functionalization astaxanthin nano-emulsion preparation of the embodiment of the present invention
Figure.
Detailed description of the invention
As it is shown in figure 1, the present invention provides the preparation method of a kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation, it is special
Levy and be, including step: S1, astaxanthin oil is mixed to form with oil phase first mixes oil phase, and the weight of this astaxanthin oil accounts for
Less than the 40% of first mixing oil phase weight；S2, it is added to surfactant mix homogeneously at the first miscella and forms second and mix
Closing oil phase, the weight ratio of this surfactant and the first mixing oil phase is 2:3；S3, second mixing oil phase in add concentration low
In the hyaluronic acid aqueous solution of 0.2%, stirring, obtain emulsion oil-in-water, wherein the weight of this hyaluronic acid aqueous solution accounts for
The 90% of gross weight.
When the first oil phase, surfactant, the ratio of hyaluronic acid aqueous solution are fixed, the particle diameter of the emulsion of preparation is one
Fixed, if increasing the ratio of astaxanthin oil, then can change particle diameter, so the content of astaxanthin oil is below 40%, more preferably
2%-40%；And the mass ratio that hyaluronic acid is in the solution is not above 0.2%, if hyaluronic acid concentration is too high, breast can be affected equally
The particle diameter of liquid, particle diameter increase can cause system to reduce the degree of protection of astaxanthin, affect nanoemulsion transdermal effect.
In the present embodiment, the weight of astaxanthin oil accounts for the 2% of the first mixing oil phase weight, and this astaxanthin oil Prawn is blue or green
Cellulose content is 5%.In this step S3, when adding hyaluronic acid aqueous solution in this second mixing oil phase, this hyaluronic acid is water-soluble
Liquid is added dropwise over, and the solvent of this hyaluronic acid aqueous solution can use distilled water.When stirring, use magnetic agitation with regard to energy
By this second mixing oil phase stirs with hyaluronic acid aqueous solution, form homogeneous nano-emulsion system, it is not necessary to other equipment, can
To reduce production cost.
In the present embodiment, can take 0.2g～4g content astaxanthin is that 5% astaxanthin oil mixes shape with 9.8g～6g oil phase
Becoming the first mixing oil phase, the gross mass of this first mixing oil phase is 10g, then takes 0.6g first and mixes oil phase, adds 0.4g non-
Formation the second mixing oil phase, the then compound concentration hyalomitome less than 0.2% it is uniformly mixed under ionic surface active agent room temperature
Aqueous acid, when preparation, can weigh 0.01g～0.02g hyaluronic acid and be dissolved in 10g distilled water, stirs into homogeneous saturating
Bright matter acid solution；Finally being added dropwise over the concentration hyaluronic acid aqueous solution 9g less than 0.2% in the second mixing oil phase, stirring is all
Even, obtain homogeneous hyaluronic acid functionalization astaxanthin nano-emulsion after stirring, whole system presents homogeneous redness.This preparation side
Method component is simple, and operation is easy to implement, and has prepared the oil-in-water type nanoemulsions that particle diameter is tiny, long-term dynamics is stable, tool
Have that energy consumption is little, low cost, can the feature such as commercial production.
The preparation method of astaxanthin nano-emulsion preparation that the present invention provides, it is not necessary to use high pressure homogenizer or ultrasonic broken
Broken instrument inputs energy from the external world, it is not necessary to High Temperature High Pressure, is well mixed with astaxanthin oil by hydrophobic oil phase simultaneously, it is not necessary to
Introduce organic solvent, and astaxanthin is embedded in close spherical emulsion, it is possible to well provide protective effect to astaxanthin.Simultaneously
Introducing hyaluronic acid aqueous solution makes the speed through epidermis of astaxanthin nano-emulsion be improved, and more improves the heat-resisting of astaxanthin
Property, it is seen that the preparation not only method of the astaxanthin nano-emulsion preparation that the present invention provides is simple, can be with industrialized production, and can be significantly
Reduce production cost.
The present invention also provides for a kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation, is the first mixing oil phase, surface work
Property agent, concentration less than 0.2% hyaluronic acid aqueous solution formed emulsion oil-in-water, wherein, the weight of this hyaluronic acid aqueous solution
Amount accounts for the 90% of gross weight, and this first mixing oil phase, the weight ratio of surfactant are 3:2；This first mixing oil phase includes that shrimp is blue or green
Vegetable oil and oil phase, and the weight of this astaxanthin oil account for this first mixing oil phase weight less than 40%.In the present embodiment, this shrimp
The weight of blue or green vegetable oil accounts for the 2% of this first mixing oil phase weight, and in this astaxanthin oil, content astaxanthin is 5%, this hyaluronic acid
The solvent of aqueous solution can use distilled water.
The present invention provides a kind of hyaluronic acid functionalization astaxanthin nano-emulsion preparation and preparation method thereof, and utilize emulsion has
The mutually hydrophobic feature of machine, is wrapped in hydrophobic astaxanthin oil in oil phase, it is to avoid the use of acetone and other organic solvent；Utilize
The method of mental retardation nano-emulsion, is embedded in astaxanthin in nano-emulsion, improves the stability of astaxanthin, solves astaxanthin real
Can not the problem of industrialization in the application of border；It is simultaneously introduced hyaluronic acid aqueous solution and improves the speed through epidermis of astaxanthin nano-emulsion
Rate；The hyaluronic acid functionalization astaxanthin nano-emulsion preparation that the application present invention obtains has higher stability and through epidermis
Speed.
Illustrating the preferred embodiments of the present invention above by reference to accompanying drawing, those skilled in the art are without departing from the scope of the present invention
And essence, multiple flexible program can be had to realize the present invention.For example, as the shown partially of an embodiment or describe
Feature can be used for another embodiment to obtain another embodiment.These are only the embodiment that the present invention is the most feasible, not
Therefore the interest field of the present invention is limited to, the equivalence change that all utilization description of the invention and accompanying drawing content are made, it is both contained in
Within the interest field of the present invention.
Claims (2)
1. the preparation method of a hyaluronic acid functionalization astaxanthin nano-emulsion preparation, it is characterised in that include step:
S1, astaxanthin oil is mixed to form with oil phase first mixes oil phase, and the weight of described astaxanthin oil is described first to mix
Close less than the 40% of oil phase weight；
S2, being added to surfactant mix homogeneously at described first miscella and form the second mixing oil phase, lives in described surface
The weight ratio that property agent mixes oil phase with described first is 2:3；
S3, mix described second oil phase adds the concentration hyaluronic acid aqueous solution less than 0.2%, stir, wherein said
The weight of hyaluronic acid aqueous solution accounts for the 90% of gross weight；
In described step S1, in described astaxanthin oil, content astaxanthin is 5%；
In described step S1, the weight of described astaxanthin oil is the 2% of described first mixing oil phase weight；
In described step S3, when described second mixes and add hyaluronic acid aqueous solution in oil phase, described hyaluronic acid is water-soluble
Liquid is added dropwise over；
Solvent in described hyaluronic acid aqueous solution is distilled water；
In described step S3, magnetic agitation is used to be stirred with hyaluronic acid aqueous solution by described second mixing oil phase.
2. a hyaluronic acid functionalization astaxanthin nano-emulsion preparation, it is characterised in that be the first mixing oil phase, surface activity
The emulsion oil-in-water that agent, the concentration hyaluronic acid aqueous solution less than 0.2% are formed, wherein, the weight of described hyaluronic acid aqueous solution
Amount accounts for the 90% of gross weight, and described first mixing oil phase, the weight ratio of surfactant are 3:2；Described first mixing oil phase includes
Astaxanthin oil and oil phase, and less than 40% that the weight of described astaxanthin oil is described first mixing oil phase weight；Described astaxanthin
In oil, content astaxanthin is 5%；Solvent in described hyaluronic acid aqueous solution is distilled water；The weight of described astaxanthin oil is institute
State the 2% of the first mixing oil phase weight.
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