CN104012775A - Method for hydrolyzing vegetable protein - Google Patents
Method for hydrolyzing vegetable protein Download PDFInfo
- Publication number
- CN104012775A CN104012775A CN201410299812.0A CN201410299812A CN104012775A CN 104012775 A CN104012775 A CN 104012775A CN 201410299812 A CN201410299812 A CN 201410299812A CN 104012775 A CN104012775 A CN 104012775A
- Authority
- CN
- China
- Prior art keywords
- temperature
- hydrolysis
- add
- regulate
- plant protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a method for hydrolyzing vegetable protein and belongs to the field of feed processing. The method for hydrolyzing vegetable protein disclosed by the invention sequentially comprises the following steps: adding a mixture of enzymes including alpha-amylase, cellulose, hemicellulase, pectinase, alpha-galactosidase and phytase at a mass ratio of 10:5:5:2:2:1 into a vegetable protein raw material, and hydrolyzing for 2-5 hours, wherein the addition amount of the mixture is 3-8 g/Kg of the vegetable protein raw material; adding neutral protease, and hydrolyzing for 2-5 hours, wherein the addition amount of the neutral protease is 2-4 g/kg of the vegetable protein raw material; adding alkaline protease, and hydrolyzing for 2-5 hours, wherein the addition amount of the alkaline protease is 3-5 g/kg of the vegetable protein raw material; drying to obtain powdery vegetable hydrated protein. The method for hydrolyzing vegetable protein disclosed by the invention is capable of hydrolyzing a plurality of plant protein raw materials and reducing nutrient levels in the plant raw materials and is thorough in the hydrolyzing process.
Description
Technical field
The invention belongs to field of feed processing, relate in particular to the method that uses multiple hydrolase fractional hydrolysis vegetable protein to produce feedstuff.
Background technology
Along with the domestic and global increase to meat products demand, the also sustainable growth of the demand of protide feedstuff.But animal protein raw material output does not roll up, and owing to worrying the transmissions of disease such as rabid ox disease, the use amount of animal protein raw material is also reducing.The use amount sustainable growth of plant protein material, but due to the result of plant evolution selection, plant protein material contains multiple ANFs, therefore must carry out pretreatment or adopt the method that reduces addition to reduce the generation of stress reaction plant protein material.Except adopting thermal denaturation with inactivating protein class ANFs, adopting hydrolase hydrolyzed vegetable protein raw material is also a kind of method that reduces very efficiently ANFs.In the application study in cultivation about hydrolyzed vegetable protein, can reduce stress reaction except reducing ANFs, feed can also improve after hydrolyzed vegetable protein animal immunocompetence, digestion power, improve the quality of meat.The people such as Zhou (Effect of Feeding Enzymolytic Soybean Meal on Performance, Digestion and Immunity of Weaned Pigs, AJAS, 2011,24:101-109) in weaning pigs, add enzymatic hydrolysis of soybean albumen and can promote piglet growth afterwards, improve digestion power and immunity.The people such as Wang (Effect of enzymolytic soybean meal on growth performance, nutrient digestibility and immune function of growing broilers, Animal Feed Science and Technology, 2011,169:224-229) prove that feed intake, daily gain, feedstuff-meat ratio, digestibility and the immunity of broiler chicken are all directly proportional to the addition of enzymatic hydrolysis of soybean albumen in feed.The people such as Gui (Effects of different dietary levels of cottonseed meal protein hydrolysate on growth, digestibility, body composition and serum biochemical indices in crucian carp (Carassius auratus gibelio), 2010,156:112-120) find to add in carp feed 5% enzyme hydrolysis cotton protein and can improve body weight and digestibility, also can increase the content of albumen, zinc and iron in the flesh of fish.Therefore, large-scale production hydrolyzed vegetable protein has very important economic worth and social value.
In prior art, have the production method of some vegetable protein hydrolyzates, but these methods are mainly used in food and medicine field, can not meet the production requirement of feedstuff.In addition, method of the prior art also only can be applicable to a certain plant, and hydrolytic process also can only be processed the ANFs of protide, can not process more extensively and profoundly other ANFs.
Summary of the invention
The object of the invention is to: a kind of method of hydrolyzed vegetable protein raw material is provided, can be hydrolyzed various plants protein raw materials, hydrolytic process is thorough, can reduce the ANFs level in plant material.
The object of the invention realizes by following technical proposals:
A method for hydrolyzed vegetable protein, comprises the following steps successively:
A) take plant protein material, the temperature that adds relative material quality 5-8 times is the water of 90-100 DEG C, stirs, and maintains more than 90 DEG C 20-30min;
B) allow temperature be cooled to 50-60 DEG C, regulate while stirring pH to 3-5, temperature maintains 50-60 DEG C;
C) after pH and temperature stabilization, add the mixture of following enzyme: mass ratio is AMS: cellulase: hemicellulase: pectase: alpha-galactosidase: phytase=10:5:5:2:2:1, mixture addition is 3-8g/kg plant protein material;
D) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
E) regulate pH to 6-8, temperature is adjusted to 55-65 DEG C;
F) after pH and temperature stabilization, add neutral proteinase, addition is 2-4g/kg plant protein material;
G) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
H) regulate pH to 8-9, temperature is adjusted to 60-70 DEG C;
I) after pH and temperature stabilization, add alkali protease, addition is 3-5g/kg plant protein material;
J) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
K) regulate pH to 6-8;
L) enzymolysis product is done after high-speed homogenization processing, spray dry, obtain powdery plant protein hydrolysate.
As selection, described plant protein material is selected from one or more in dregs of beans, cottonseed, wheat and rice.
As selection, in described B, K step, add lactic acid or salt acid for adjusting pH.
As selection, in described E, H step, add calcium hydroxide or NaOH to regulate pH.
As further selection, the method for described hydrolyzed vegetable protein, comprises the following steps successively:
A) take plant protein material, the temperature that adds relative material quality 6-8 times is the water of 90-100 DEG C, stirs, and maintains more than 90 DEG C 30min;
B) allow temperature be cooled to 55 DEG C, regulate while stirring pH to 4.0-4.5, temperature maintains 55 DEG C;
C) after pH and temperature stabilization, add the mixture of following enzyme: mass ratio is AMS: cellulase: hemicellulase: pectase: alpha-galactosidase: phytase=10:5:5:2:2:1, mixture addition is 6-6.5g/kg plant protein material;
D) maintain pH and temperature stabilization, hydrolysis 3-4 hour;
E) regulate pH to 7.0-7.5, temperature is adjusted to 60 DEG C;
F) after pH and temperature stabilization, add neutral proteinase, addition is 3g/kg plant protein material;
G) maintain pH and temperature stabilization, when hydrolysis 3-5;
H) regulate pH to 8.5-9.0, temperature is adjusted to 70 DEG C;
I) after pH and temperature stabilization, add alkali protease, addition is 4g/kg plant protein material;
J) maintain pH and temperature stabilization, hydrolysis 3-5 hour;
K) regulate pH to 7-8;
L) enzymolysis product is done after high-speed homogenization processing, spray dry, obtain powdery plant protein hydrolysate.
The main scheme of aforementioned the present invention and each further selection scheme thereof can independent assortment to form multiple schemes; be the present invention and can adopt also claimed scheme: as the present invention; each selection can be selected any combination with other; those skilled in the art can understand there is multiple combination according to prior art and common practise after understanding the present invention program; be the claimed technical scheme of the present invention, do not do exhaustive at this.
The inventor finds in research work, after adding AMS, cellulase, pectase and hemicellulase, can effectively improve protein hydrolysis degree, improve product quality, and further add alpha-galactosidase and phytase can be hydrolyzed raffinose, stachyose and phytic acid, can further effectively reduce ANFs content.
Adding after AMS, cellulase, pectase and hemicellulase prehydrolysis plant protein material, significantly improve protein hydrolysis degree; Also increase scheme applicability, be not only applicable to bean cake powder, also can be used for the hydrolysis of cottonseed protein, gluten powder and rice protein powder.As accompanying drawing one, after hydrolysis, the polypeptide molecular weight of soybean, cottonseed, wheat, rice is mainly distributed in 2kDa between 15kDa.
Add after alpha-galactosidase, the stachyose in plant protein material and raffinose content have reduced 70%-90%; Add after phytase, the phytic acid in raw material has reduced 60%-80%, and this has significantly reduced the ANFs level in raw material, improves the production performance of animal.
In the hydrolysate of dregs of beans, protide ANFs glycinin has reduced 60%-90%, and beta-conglycinin has reduced 55%-85%, and trypsin ihhibitor has reduced 60%-95%, show that this technique is suitable for the processing of plant-derived albumen, proteolysis is more thorough.
In concrete scheme, take multistep hydrolysis scheme, first hot water environment makes albuminous degeneration, add AMS, cellulase, pectase and hemicellulase prehydrolysis plant protein material and add alpha-galactosidase and phytase hydrolysis raffinose, stachyose and phytic acid reduction ANFs content, carry out successively more afterwards the hydrolysis of neutral proteinase and alkali protease.Specific hydrolysis order, enzyme addition and pH, temperature and time control, make finally to obtain best hydrolysis effect.
Beneficial effect of the present invention: adaptability is wider, can be hydrolyzed various plants protein raw materials; Hydrolytic process is thorough, and product is mainly the polypeptide of 100 following amino acid residue compositions; Can reduce the various ANFs levels in plant material, the possibility of stress reaction occurs to reduce animal ingestion afterwards; Can carry out large-scale industrial production, meet the requirement that feedstuff is produced.
Brief description of the drawings
Fig. 1 is the PAGE electrophoretogram of Four Plants protolysate of the present invention;
Wherein 1, dregs of beans, 2, dregs of beans hydrolysate, 3, cottonseed protein hydrolysate, 4, gluten powder hydrolysate, 5, rice protein powder hydrolysate.
Detailed description of the invention
Following non-limiting examples is used for illustrating the present invention.
Comparative example 1:
Take bean cake powder 100g, add water 500ml, temperature is adjusted to 55 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 0.2g, hydrolysis 2h.Temperature is adjusted to 65 DEG C, and pH adjusts to 8.0-8.5, adds alkali protease 0.4g, hydrolysis 2h.Drying and crushing, measuring protein hydrolysis degree is 44.5%.
Comparative example 2:
Take bean cake powder 100g, add water 500ml, temperature is adjusted to 55 DEG C, and pH adjusts to 3.5-4.Add AMS 0.2g, hydrolysis 2h.Temperature is adjusted to 55 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 0.2g, hydrolysis 2h.Temperature is adjusted to 65 DEG C, and pH adjusts to 8.0-8.5, adds alkali protease 0.4g, hydrolysis 2h.Drying and crushing, measuring protein hydrolysis degree is 52.4%.
Comparative example 3:
Take bean cake powder 100g, add water 600ml, temperature is adjusted to 50 DEG C, and pH adjusts to 4-4.5.Add the mixture of AMS, cellulase, hemicellulase, pectase, mass ratio is 10:5:5:2,0.4g altogether, hydrolysis 3h.Temperature is adjusted to 60 DEG C, and pH adjusts to 6.5-7.0, adds neutral proteinase 0.2g, hydrolysis 3h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 0.5g, hydrolysis 3h.Drying and crushing, measuring protein hydrolysis degree is 65.6%.
Comparative example 4:
Take bean cake powder 100g, add boiling water 600ml, maintain more than 90 DEG C half an hour.Temperature is adjusted to 55 DEG C, and pH4-4.5 adds AMS, cellulase, hemicellulase, pectase mixture, and mass ratio is 10:5:5:2,0.6g altogether, hydrolysis 4h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 0.3g, hydrolysis 3h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 0.4g, hydrolysis 3h.Drying and crushing, measuring protein hydrolysis degree is 72.9%.
Embodiment 1:
Take bean cake powder 100g, add boiling water 600ml, maintain more than 90 DEG C half an hour.Temperature is adjusted to 55 DEG C, and pH4-4.5 adds the mixture of AMS, cellulase, hemicellulase, pectase, alpha-galactosidase, phytase, and mass ratio is 10:5:5:2:2:1,0.65g altogether, hydrolysis 4h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 0.3g, hydrolysis 3h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 0.4g, hydrolysis 3h.Drying and crushing, measuring protein hydrolysis degree is 71.7%.Adopt HPLC method to measure the content of stachyose and raffinose, the content of stachyose and raffinose is respectively 1.0mg/g and 6.2mg/g, and with respect to comparative example 4, content has reduced respectively 90% and 72%.Adopt GB/T5009.153-2003 to measure phytic acid content, the phytic acid content of hydrolyzed vegetable protein is 3mg/g, and with respect to comparative example 4, phytic acid content has reduced by 73%.
Embodiment 2:
Take bean cake powder 1000kg and put in reactor, add 90-100 DEG C of hot water 6000kg, maintain 90 DEG C above 30 minutes.Temperature is adjusted to 55 DEG C, and pH adjusts to 4-4.5.Add the compound of AMS, cellulase, hemicellulase, pectase, alpha-galactosidase, phytase, mass ratio is 10:5:5:2:2:1,6.5kg altogether, hydrolysis 3h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 3kg, hydrolysis 3h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 4kg, hydrolysis 3h.PH adjusts to 7-8, and after homogenate, spraying is dried, and measuring protein hydrolysis degree is 67.2%.
Embodiment 3:
Take gluten powder 1000kg and put in reactor, add 90-100 DEG C of hot water 8000kg, maintain 90 DEG C above 30 minutes.Temperature is adjusted to 55 DEG C, pH4-4.5.Add the compound of AMS, cellulase, hemicellulase, pectase, alpha-galactosidase, phytase, mass ratio is 10:5:5:2:2:1,6kg altogether, hydrolysis 3h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 3kg, hydrolysis 3h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 4kg, hydrolysis 5h.PH adjusts to 7-8, and after homogenate, spraying is dried, and measuring protein hydrolysis degree is 62.4%.
Embodiment 4:
Take rice protein powder 1000kg and put in reactor, add 90-100 DEG C of hot water 6000kg, maintain 90 DEG C above 30 minutes.Temperature is adjusted to 55 DEG C, pH4-4.5.Add the compound of AMS, cellulase, hemicellulase, pectase, alpha-galactosidase, phytase, mass ratio is 10:5:5:2:2:1,6kg altogether, hydrolysis 3h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 3kg, hydrolysis 5h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 4kg, hydrolysis 4h.PH adjusts to 7-8, and after homogenate, spraying is dried, and measuring protein hydrolysis degree is 58.1%.
Embodiment 5:
Take cottonseed flour 1000kg and put in reactor, add 90-100 DEG C of hot water 7000kg, maintain 90 DEG C above 30 minutes.Temperature is adjusted to 55 DEG C, pH4-4.5.Add the compound of AMS, cellulase, hemicellulase, pectase, alpha-galactosidase, phytase, mass ratio is 10:5:5:2:2:1,6.5kg altogether, hydrolysis 3h.Temperature is adjusted to 60 DEG C, and pH adjusts to 7.0-7.5, adds neutral proteinase 3kg, hydrolysis 4h.Temperature is adjusted to 70 DEG C, and pH adjusts to 8.5-9.0, adds alkali protease 4kg, hydrolysis 4h.PH adjusts to 7-8, and after homogenate, spraying is dried, and measuring protein hydrolysis degree is 69.2%.
Aforementioned hydrolyzed vegetable protein degree of hydrolysis is measured.Soluble protein adopts trichloroacetic acid method to measure, and concrete operations are as follows: take approximately 2 to 3 grams, sample, be placed in beaker, adding distil water 75mL, electromagnetic agitation 45 minutes under room temperature.Solution in beaker and precipitation are shaken up, fold in centrifuge tube, under rotating speed 4000r/min centrifugal 10 minutes, collect supernatant A.Get supernatant A 25mL, add the trichloroacetic acid solution of 25mL20%, after mixing, leave standstill 20 minutes.Solution in beaker and precipitation are shaken up, fold in centrifuge tube, under rotating speed 4000r/min centrifugal 10 minutes, collect supernatant B.Get supernatant B25mL, adopt Kjeldahl nitrogen determination protein content.Adopt GB/T6432 1 to measure the content of crude protein.
Degree of hydrolysis=soluble protein content/crude protein content × 100%.
Aforementioned base materials is commercially available product, and various enzymes are purchased from DSM, Su Kehan (Weifang) bioengineering Co., Ltd, Ningxia Sunson Industrial Group Co., Ltd..
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. a method for hydrolyzed vegetable protein, is characterized in that comprising the following steps successively:
A) take plant protein material, the temperature that adds relative material quality 5-8 times is the water of 90-100 DEG C, stirs, and maintains more than 90 DEG C 20-30min;
B) allow temperature be cooled to 50-60 DEG C, regulate while stirring pH to 3-5, temperature maintains 50-60 DEG C;
C) after pH and temperature stabilization, add the mixture of following enzyme: mass ratio is AMS: cellulase: hemicellulase: pectase: alpha-galactosidase: phytase=10:5:5:2:2:1, mixture addition is 3-8g/kg plant protein material;
D) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
E) regulate pH to 6-8, temperature is adjusted to 55-65 DEG C;
F) after pH and temperature stabilization, add neutral proteinase, addition is 2-4g/kg plant protein material;
G) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
H) regulate pH to 8-9, temperature is adjusted to 60-70 DEG C;
I) after pH and temperature stabilization, add alkali protease, addition is 3-5g/kg plant protein material;
J) maintain pH and temperature stabilization, hydrolysis 2-5 hour;
K) regulate pH to 6-8;
L) enzymolysis product is done after high-speed homogenization processing, spray dry, obtain powdery plant protein hydrolysate.
2. the method for hydrolyzed vegetable protein as claimed in claim 1, is characterized in that: described plant protein material is selected from one or more in dregs of beans, cottonseed, wheat and rice.
3. the method for hydrolyzed vegetable protein as claimed in claim 1, is characterized in that: in described B, K step, add lactic acid or salt acid for adjusting pH.
4. the method for hydrolyzed vegetable protein as claimed in claim 1, is characterized in that: in described E, H step, add calcium hydroxide or NaOH to regulate pH.
5. the method for the hydrolyzed vegetable protein as described in claim 1,2,3 or 4, is characterized in that comprising the following steps successively:
A) take plant protein material, the temperature that adds relative material quality 6-8 times is the water of 90-100 DEG C, stirs, and maintains more than 90 DEG C 30min;
B) allow temperature be cooled to 55 DEG C, regulate while stirring pH to 4.0-4.5, temperature maintains 55 DEG C;
C) after pH and temperature stabilization, add the mixture of following enzyme: mass ratio is AMS: cellulase: hemicellulase: pectase: alpha-galactosidase: phytase=10:5:5:2:2:1, mixture addition is 6-6.5g/kg plant protein material;
D) maintain pH and temperature stabilization, hydrolysis 3-4 hour;
E) regulate pH to 7.0-7.5, temperature is adjusted to 60 DEG C;
F) after pH and temperature stabilization, add neutral proteinase, addition is 3g/kg plant protein material;
G) maintain pH and temperature stabilization, hydrolysis 3-5 hour;
H) regulate pH to 8.5-9.0, temperature is adjusted to 70 DEG C;
I) after pH and temperature stabilization, add alkali protease, addition is 4g/kg plant protein material;
J) maintain pH and temperature stabilization, hydrolysis 3-5 hour;
K) regulate pH to 7-8;
L) enzymolysis product is done after high-speed homogenization processing, spray dry, obtain powdery plant protein hydrolysate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410299812.0A CN104012775B (en) | 2014-06-27 | 2014-06-27 | A kind of method of hydrolyzed vegetable protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410299812.0A CN104012775B (en) | 2014-06-27 | 2014-06-27 | A kind of method of hydrolyzed vegetable protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104012775A true CN104012775A (en) | 2014-09-03 |
| CN104012775B CN104012775B (en) | 2016-01-13 |
Family
ID=51430005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410299812.0A Active CN104012775B (en) | 2014-06-27 | 2014-06-27 | A kind of method of hydrolyzed vegetable protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104012775B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104664052A (en) * | 2015-02-05 | 2015-06-03 | 江苏三仪动物营养科技有限公司 | Novel functional biological feed production process |
| CN105524966A (en) * | 2016-02-26 | 2016-04-27 | 天津现代职业技术学院 | Method for preparing ACE inhibitory peptides through bean pulp enzymolysis |
| CN106417891A (en) * | 2015-08-05 | 2017-02-22 | 江苏爱福特科技开发有限公司 | Method for preparing gluten powder hydrolysate by utilizing enzymatic method |
| CN106701876A (en) * | 2017-01-06 | 2017-05-24 | 成都美溢德生物技术有限公司 | Proteinate high-efficiency cleaning production method |
| CN106831003A (en) * | 2017-03-03 | 2017-06-13 | 杭州威斯诺威科技有限公司 | A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer |
| CN106922948A (en) * | 2015-12-29 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | The production technology of fermented bean dregs is carried out suitable for edible fat production factory environment |
| CN106982982A (en) * | 2017-05-09 | 2017-07-28 | 北京工商大学 | It is a kind of to improve the alkali heat-treatment method that vegetable protein digests efficiency |
| CN107569672A (en) * | 2016-07-05 | 2018-01-12 | 陈栋梁 | A kind of anti-tumor compositions containing Antrodia camphorata peptide and preparation method and application |
| CN107801689A (en) * | 2017-11-22 | 2018-03-16 | 恭城福茂生油茶文化产业发展有限公司 | A kind of dragon wins the disease-resistant cultural method of emerald green duck |
| CN107950470A (en) * | 2017-11-22 | 2018-04-24 | 恭城福茂生油茶文化产业发展有限公司 | A kind of high-efficiency breeding method of the emerald green duck of dragon victory |
| CN110106220A (en) * | 2019-05-24 | 2019-08-09 | 湖南华诚生物资源股份有限公司 | The method of small peptide albumen is prepared from mango seed |
| CN111528342A (en) * | 2020-06-15 | 2020-08-14 | 张国建 | Enzymolysis composition, soybean meal enzymolysis method and preparation method of organic trace element feed additive |
| CN115226862A (en) * | 2022-06-23 | 2022-10-25 | 北京宝贝优鲜科技有限公司 | Fish double-protein peptide and preparation method and composition thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096696A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process |
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
-
2014
- 2014-06-27 CN CN201410299812.0A patent/CN104012775B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096696A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process |
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
Non-Patent Citations (2)
| Title |
|---|
| 化学工业出版社组织编写: "《中国化工产品大全 下卷》", 31 January 2005, 化学工业出版社 * |
| 王琪等: "麦胚蛋白的双酶分步水解及其产物的抗氧化活性研究", 《食品科技》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104664052A (en) * | 2015-02-05 | 2015-06-03 | 江苏三仪动物营养科技有限公司 | Novel functional biological feed production process |
| CN106417891A (en) * | 2015-08-05 | 2017-02-22 | 江苏爱福特科技开发有限公司 | Method for preparing gluten powder hydrolysate by utilizing enzymatic method |
| CN106922948A (en) * | 2015-12-29 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | The production technology of fermented bean dregs is carried out suitable for edible fat production factory environment |
| CN105524966A (en) * | 2016-02-26 | 2016-04-27 | 天津现代职业技术学院 | Method for preparing ACE inhibitory peptides through bean pulp enzymolysis |
| CN107569672A (en) * | 2016-07-05 | 2018-01-12 | 陈栋梁 | A kind of anti-tumor compositions containing Antrodia camphorata peptide and preparation method and application |
| CN106701876A (en) * | 2017-01-06 | 2017-05-24 | 成都美溢德生物技术有限公司 | Proteinate high-efficiency cleaning production method |
| CN106831003A (en) * | 2017-03-03 | 2017-06-13 | 杭州威斯诺威科技有限公司 | A kind of preparation method of plant source amino acid polypeptide Water soluble fertilizer |
| CN106982982A (en) * | 2017-05-09 | 2017-07-28 | 北京工商大学 | It is a kind of to improve the alkali heat-treatment method that vegetable protein digests efficiency |
| CN107801689A (en) * | 2017-11-22 | 2018-03-16 | 恭城福茂生油茶文化产业发展有限公司 | A kind of dragon wins the disease-resistant cultural method of emerald green duck |
| CN107950470A (en) * | 2017-11-22 | 2018-04-24 | 恭城福茂生油茶文化产业发展有限公司 | A kind of high-efficiency breeding method of the emerald green duck of dragon victory |
| CN110106220A (en) * | 2019-05-24 | 2019-08-09 | 湖南华诚生物资源股份有限公司 | The method of small peptide albumen is prepared from mango seed |
| CN111528342A (en) * | 2020-06-15 | 2020-08-14 | 张国建 | Enzymolysis composition, soybean meal enzymolysis method and preparation method of organic trace element feed additive |
| CN115226862A (en) * | 2022-06-23 | 2022-10-25 | 北京宝贝优鲜科技有限公司 | Fish double-protein peptide and preparation method and composition thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104012775B (en) | 2016-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104012775B (en) | A kind of method of hydrolyzed vegetable protein | |
| CN101627793B (en) | Industrial production method of hydrolyzed wheat protein for feeding | |
| CN102948614B (en) | Method for preparing peptide used for active feed by bacteria and enzyme synergistic fermentation bean pulp | |
| CN104884615B (en) | Polypeptide with proteinase activity | |
| AU2015341922B2 (en) | Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed | |
| CN103734542A (en) | Fermented feed for nursery pig and preparation method thereof | |
| CN102669447B (en) | Low-protein feed for small broiler chicken and preparation method thereof | |
| CN103907758B (en) | The preparation method of a kind of feeding fermented type bean sprouts feed | |
| CA3037875A1 (en) | Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion | |
| CN103931885A (en) | Preparation method of acidified small-peptide protein feed | |
| JP6017571B2 (en) | Method for producing fermented corn gluten | |
| CN103478438B (en) | Mixed feed for mixotrophic culture of litopenaeus vannamei and sparus latus and preparation method thereof | |
| CN104686833A (en) | High-yield and disease-resistant turtle feed | |
| CN103766609B (en) | Preparation method of polypeptide feed additive | |
| CA2997213C (en) | Glycoside hydolases and their use in preventing and/or treating a pathogenic infection in an animal | |
| CN105558411A (en) | High-protein fish feed | |
| CN103988981A (en) | Environmentally-friendly low cost pig feed | |
| CN110663841A (en) | Broiler feed and application thereof | |
| CN103416592A (en) | Industrial production method of rice protein hydrolysate for feed | |
| Sorbara et al. | Enzymatic programs for broilers | |
| CN104206866A (en) | Making method of chicken feed for improving feed conversion rate | |
| CN103564226A (en) | Manufacturing method of chicken feed capable of improving egg yield | |
| CN104150975A (en) | Marine edible and medicinal fungus culture medium and preparation method thereof | |
| CN105941979A (en) | Feed for accelerating goose growth by modified sterculia nobilis seed meal and preparation method of feed | |
| CN103283964A (en) | Enzymed soy albumen preparation for feed and preparation method of enzymed soy albumen preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |