CN104004688A - Preparation method of compound zymophyte yeast for pickled pepper - Google Patents

Abstract

The invention relates to a preparation method of compound zymophyte yeast for pickled pepper. The preparation method comprises the following specific steps: firstly preparing a compound strain, a bacterial culture medium, a compound bacteria leavening agent and long grain rice vermicelli; then adding the leavening agent, the final concentration of which reaches total viable bacteria of 10<7>-10<9>CFU/ml, the sterilized long grain rice vermicelli and 3% of maltose water to a blender, fully blending, uniformly mixing, making a mixture into soft materials, cutting the soft materials into 8cm*5cm*3cm blocks by using a cutter, placing the blocks in a fermentation room, fermenting at the temperature of 28-30 DEG C for 72-96 hours, after ending fermentation, transferring to a clean air dry oven, and drying for 96 hours at the temperature of 35 DEG C so as to obtain the compound zymophyte yeast; and sealing and packaging the compound zymophyte yeast by using sterile plastic bags, then placing in a 2-5 DEG C ice tanks and storing. The compound zymophyte yeast produced by the method is long in storage time and very convenient to use.

Description

A kind of preparation method of the composite zymocyte song for pickling pepper

Technical field

The invention belongs to food processing field, particularly a kind of preparation method of the composite zymocyte song for pickling pepper.

Background technology

Pickling pepper is a kind of pickled product processing with hygrometric state fermentation mode, generally need to pickle two weeks left and right.Simultaneously pickling pepper is also modal a kind of seasoning matter on common people's dining table, and it is nutritious, and special taste can increase appetite while having dinner, and many foods can physical strength reinforcing, improve be afraid of cold, the symptom such as frostbite, vascular headache.In capsicum, contain the different material of a kind of thing simultaneously, can accelerate metabolism, promote hormone secretion, skin care.The Vc being rich in, can control heart trouble and coronary sclerosis, reduces cholesterol.Contain more antioxidant, can preventing cancer and other chronic diseases.Different local pickling peppers differ from one another, and brewed local flavor and the mouthfeel going out is far from each other.

Summary of the invention

The preparation method who the object of this invention is to provide a kind of composite zymocyte song using for pickling pepper fermenting process.

For a preparation method for the composite zymocyte song of pickling pepper, this preparation method's concrete steps are:

(1), composite bacteria preparation: select commercially available plant lactobacillus and Leuconostoc mesenteroides bacterial strain, be mixed and made into composite bacteria by weight 1:2;

(2), bacteria culture medium preparation: peptone 10 g, yeast extract 5 g, dibasic ammonium citrate 2 g, glucose 20 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, magnesium sulfate heptahydrate 0.58 g, four water manganous sulfate 0.25 g are inserted in the container that 1000 ml distilled water are housed, and making its pH value is 6.5; Seed fermentation nutrient solution ratio: peptone 10 g, yeast extract 5 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, vegetables juice 200 ml, distilled water 800 ml;

(3), compound bacteria-fermented agent preparation: under aseptic condition, by bacteria culture medium tube activation for above-mentioned composite bacteria bacterial classification, be inoculated on strain expanded culture base, at 30 ℃ of temperature, cultivate 36h, enter seed fermentation nutrient solution, the middle fermented liquid of access 3%～5% is cultivated productive leavening agent, and it is l0 that starter ultimate density reaches total viable count ⁷～10 ⁹cFU/ml;

(4), long-grained nonglutinous rice powder is prepared: long-grained nonglutinous rice is pulverized, crossed 70 mesh sieves standby, in steamer, dry blowing is 30 minutes, is cooled to 28～30 ℃, standby;

(5), the preparation of composite zymocyte song: it is l0 that ultimate density is reached to total viable count ⁷～10 ⁹the starter of CFU/ml adds in stirrer with the long-grained nonglutinous rice powder after sterilizing and 3% maltose water, fully be uniformly mixed, make softwood, be cut into softwood by knife 8cm * 5cm * 3cm block, put into sweathouse 28～30 ℃ of fermentations 72～96 hours, in the clean air dry oven of the rear immigration of ferment, at 35 ℃, be dried 96 hours and get final product;

(6), packing: the composite zymocyte song being up to the standards is packed by 500g/ bag with aseptic plastic bag;

(7), storage: the packing bag of dress composite zymocyte song is placed in to 2～5 ℃ of refrigerator-freezers and preserves.

The zymophyte that adopts the present invention to produce is bent, not only the shelf time long, and very easy to use, during use, only zymophyte song need be placed in baking oven and keep certain temperature and time just can make flora activate, reach service requirements.

Embodiment

Pickling pepper is the chilli products that a kind of people like, particularly in southwest, owing to having a humid climate, people, no matter be cooking or blocked shot, like putting capsicum, both can whet the appetite, and can dispel cold again.To be people put into soaking fermentation in pickle jar by fresh chilli is peace and quiet to traditional pickling pepper, ediblely how much takes out how many.If but large-scale production can not be adopted in this way.

Technician is according to years'experiences, develops a kind of composite zymocyte of using in pickling pepper fermenting process of being applicable to bent, and its preparation method is:

(1), composite bacteria preparation: select commercially available plant lactobacillus and Leuconostoc mesenteroides bacterial strain, be mixed and made into composite bacteria by weight 1:2;

(2), bacteria culture medium preparation: peptone 10 g, yeast extract 5 g, dibasic ammonium citrate 2 g, glucose 20 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, magnesium sulfate heptahydrate 0.58 g, four water manganous sulfate 0.25 g are inserted in the container that 1000 ml distilled water are housed, and making its pH value is 6.5; Seed fermentation nutrient solution ratio: peptone 10 g, yeast extract 5 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, vegetables juice 200 ml, distilled water 800 ml;

(3), compound bacteria-fermented agent preparation: under aseptic condition, by bacteria culture medium tube activation for above-mentioned composite bacteria bacterial classification, be inoculated on strain expanded culture base, at 30 ℃ of temperature, cultivate 36h, enter seed fermentation nutrient solution, the middle fermented liquid of access 3%～5% is cultivated productive leavening agent, and it is l0 that starter ultimate density reaches total viable count ⁷～10 ⁹cFU/ml;

(4), long-grained nonglutinous rice powder is prepared: long-grained nonglutinous rice is pulverized, crossed 70 mesh sieves standby, in steamer, dry blowing is 30 minutes, is cooled to 28～30 ℃, standby;

(5), the preparation of composite zymocyte song: it is l0 that ultimate density is reached to total viable count ⁷～10 ⁹the starter of CFU/ml adds in stirrer with the long-grained nonglutinous rice powder after sterilizing and 3% maltose water, fully be uniformly mixed, make softwood, be cut into softwood by knife 8cm * 5cm * 3cm block, put into sweathouse 28～30 ℃ of fermentations 72～96 hours, in the clean air dry oven of the rear immigration of ferment, at 35 ℃, be dried 96 hours and get final product;

(6), packing: the composite zymocyte song being up to the standards is packed by 500g/ bag with aseptic plastic bag;

(7), storage: the packing bag of dress composite zymocyte song is placed in to 2～5 ℃ of refrigerator-freezers and preserves.

When needs ferment composite zymocyte song for pickling pepper, first activate, that is: the composite zymocyte song of preserving certain hour in refrigerator-freezer is activated to 36 hours in the baking oven of 28～30 ℃, recover the vigor of composite bacteria, for pickling pepper, ferment.

The present invention compares and has following characteristics with traditional natural fermentation:

Although a. traditional natural zymotechnique is simpler, but fermenting speed is affected greatly by natural temperature, the fermenting-ripening time is about 15 days, very easily accumulates the objectionable impuritiess such as nitrite, nitrosamine, nitroso compound in the pickles course of processing, can produce larger edible safety problem; And the pickling pepper lactic acid of artificial inoculation compound bacteria-fermented generates soon, thereby accelerated fermenting speed, fermenting-ripening time shorten is 2～3 days;

B. fermentation lactic acid bacteria of the present invention is preponderated, the a large amount of lactic acid that produce in the course of processing can suppress miscellaneous bacteria to be infected, simultaneously, objectionable impurities content after product maturation such as the nitrite producing in the course of processing, nitrosamine, nitroso compound are extremely low, to human body, can not cause any harm, thereby improve edible safety;

C. many materials that composite bacteria growth metabolism produces, as lactic acid, acetic acid, N.F,USP MANNITOL, 2-heptanone etc. can strengthen product special flavour again.

Therefore the pickling pepper that, uses the present invention to produce is bright in colour, give off a strong fragrance, pungent is long, local flavor is outstanding, mouthfeel is soft, is that pickling pepper is as a kind of better selection of suitability for industrialized production.Not only can overcome many deficiencies of spontaneous fermentation, but also can improve state of the art and quality product and edible safety that pickling pepper is produced.

Claims (1)

1. for a preparation method for the composite zymocyte song of pickling pepper, it is characterized in that: this preparation method's concrete steps are:

(1), composite bacteria preparation: select commercially available plant lactobacillus and Leuconostoc mesenteroides bacterial strain, be mixed and made into composite bacteria by weight 1:2;

(2), bacteria culture medium preparation: peptone 10 g, yeast extract 5 g, dibasic ammonium citrate 2 g, glucose 20 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, magnesium sulfate heptahydrate 0.58 g, four water manganous sulfate 0.25 g are inserted in the container that 1000 ml distilled water are housed, and making its pH value is 6.5; Seed fermentation nutrient solution ratio: peptone 10 g, yeast extract 5 g, extractum carnis 10 g, tween 80 ml, dipotassium hydrogen phosphate 2 g, vegetables juice 200 ml, distilled water 800 ml;

(3), compound bacteria-fermented agent preparation: under aseptic condition, by bacteria culture medium tube activation for above-mentioned composite bacteria bacterial classification, be inoculated on strain expanded culture base, at 30 ℃ of temperature, cultivate 36h, enter seed fermentation nutrient solution, the middle fermented liquid of access 3%～5% is cultivated productive leavening agent, and it is l0 that starter ultimate density reaches total viable count ⁷～10 ⁹cFU/ml;

(4), long-grained nonglutinous rice powder is prepared: long-grained nonglutinous rice is pulverized, crossed 70 mesh sieves standby, in steamer, dry blowing is 30 minutes, is cooled to 28～30 ℃, standby;

(5), the preparation of composite zymocyte song: it is l0 that ultimate density is reached to total viable count ⁷～10 ⁹the starter of CFU/ml adds in stirrer with the long-grained nonglutinous rice powder after sterilizing and 3% maltose water, fully be uniformly mixed, make softwood, be cut into softwood by knife 8cm * 5cm * 3cm block, put into sweathouse 28～30 ℃ of fermentations 72～96 hours, in the clean air dry oven of the rear immigration of ferment, at 35 ℃, be dried 96 hours and get final product;

(6), packing: the composite zymocyte song being up to the standards is packed by 500g/ bag with aseptic plastic bag;

(7), storage: the packing bag of dress composite zymocyte song is placed in to 2～5 ℃ of refrigerator-freezers and preserves.