CN104001180B - A kind of preparation method of marine alga natural antioxidant - Google Patents
A kind of preparation method of marine alga natural antioxidant Download PDFInfo
- Publication number
- CN104001180B CN104001180B CN201410206699.7A CN201410206699A CN104001180B CN 104001180 B CN104001180 B CN 104001180B CN 201410206699 A CN201410206699 A CN 201410206699A CN 104001180 B CN104001180 B CN 104001180B
- Authority
- CN
- China
- Prior art keywords
- marine alga
- dry powder
- preparation
- slag
- natural antioxidant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Edible Seaweed (AREA)
Abstract
The invention discloses a kind of preparation method of marine alga natural antioxidant.By the mashing of new fresh seaweed, broken, hot water extraction, centrifugation, dry, crush, sieving and to obtain marine alga slag, further using biological enzyme broken wall, obtain the marine alga slag dry powder after broken wall；Extracted at room temperature using food grade absolute ethyl alcohol again, collect extract solution, marine alga natural antioxidant product is obtained after concentrated under reduced pressure and vacuum drying.The product is paste, and the total phenol for containing is 5.2 13.7 mass %, and total carotinoid is 3.6 8.4 mass %, and terpenoid is 0.5 1.3 mass %, and phytosterin compound is 0.9 2.6 mass %.The present invention uses marine alga slag that the natural of strong anti-oxidative activity is prepared for raw material, realizes the comprehensive utilization of resource；Simple production process, efficiently, environmental protection is suitable to industrialized production；The oxidation resistance of product is strong, green natural, and traditional chemical can be replaced to synthesize antioxidant use in the food industry.
Description
Technical field
The present invention relates to the preparation technology field of natural, and in particular to a kind of system of marine alga natural antioxidant
Preparation Method.
Background technology
Research shows, oxidative stress is not only the DDs such as cancer, aging, angiocardiopathy and occurs, development is total to
Same pathologic basis, are also the main cause for causing or accelerating food spoilage.Traditional antioxidants mainly have TBHQ
（TBHQ）, dibutyl hydroxy toluene（BHT）, butylated hydroxy anisole（BHA）Etc. chemical substance.Current research finds that these are changed
Learn antioxidant and exist to health potentially hazardous, forbidden making in infant and infant foods in European and American developed countries
With.Therefore, the antioxidant of exploitation natural green substitutes traditional antioxidants application in the food industry into urgent need.
There are substantial amounts of Natural Antioxidants in marine alga.Polyphenols is the important polyphenoils in marine alga
Matter, its content can account for the 1-14% of marine alga dry weight, and seeweed polyphenol has relatively big difference, land in structure with terrestrial plant polyphenol
Plant polyphenol is mainly the derivative of gallic acid and ellagic acid, and seeweed polyphenol is then the polymer of phloroglucin（Holdt &
Kraan, 2011）.Research display, brown algae polyphenols are had outstanding performance in terms of the multiple biological activities such as anti-oxidant.By chemical system
And the comparing of the antioxidant measuring methods of cell system finds, the antioxidation activity of brown algae polyphenols to be much better than vitamin C and
Vitamin E（Yotsu-Yamashitaet al., 2013）.Fucoxanthin (fucoxanthin) is another important work(in marine alga
Energy active material, content can account for more than the 0.5% of marine alga dry weight.Fucoxanthin is also known as brown alga flavine, fucoxanthine, belongs to lutein
Class, its content accounts for more than the 60% of sea-tangle carotenoid total amount（Yan little Jun etc., 2001）.Chen Wenjia etc.（2012）By rock algae
Flavine is determined to the Scavenging activity of hydrogen peroxide, hydroxyl radical free radical, DPPH free radicals, it was demonstrated that its inoxidizability is apparently higher than vitamin
C；Ha etc.（2011）Research finds that fucoxanthin can effectively improve rat plasma glutathione peroxidase and catalase
Containing activity, the oxidative stress of high fat diet induction is reduced.Additionally, research display, lipid, terpene, halide in marine alga, contain
Sulphur compound etc. also has good anti-oxidation function.Therefore, extracting Natural Antioxidants using marine alga has huge opening
Make an offer value.
However, the exploitation to marine alga are mainly the polyphenoils such as algal polysaccharides, seeweed polyphenol, fucoxanthin at present
But as marine alga slag is processed as cheap feed or bio-feritlizer, the significant wastage of resource is caused.Using algal polysaccharides plus
Natural is obtained in work accessory substance-marine alga slag and still belongs to blank.Therefore, Natural Antioxidants are extracted from marine alga slag,
With highly important economic worth and realistic meaning.
The content of the invention
Extract natural from the processing byproduct-marine alga slag of algal polysaccharides is extracted it is an object of the invention to provide a kind of
The preparation method of polyphenoils.
To achieve the above object, the present invention provides a kind of preparation method of marine alga natural antioxidant, it is characterised in that
Comprise the following steps,
The preparation of marine alga slag：New fresh seaweed is beaten to without obvious bulk thing, and by volume 1:5 add 80 after deionized water
DEG C extract 1 h, centrifugation, preferably 3000 r/min, 5 min, taking precipitate, obtain marine alga slag；
Optional, can also include continuing to add deionized water toward marine alga slag, abovementioned steps are repeated, collect marine alga slag；
The preparation of marine alga slag dry powder：Marine alga slag drying, crushing, sieving are made marine alga slag dry powder；
The biological enzymolysis broken wall of marine alga slag dry powder：Deionization is added after the marine alga slag dry powder and complex enzyme formulation are mixed
Water, in certain hour is digested under normal temperature, is centrifuged, preferably 3000 r/min, 5 min, collects sediment, does for 80 DEG C in an oven
Dry 6 h, obtains the marine alga slag dry powder after broken wall；
Organic solvent is extracted：Absolute ethyl alcohol is added toward the marine alga slag dry powder after the broken wall, a timing is extracted under normal temperature
Between, centrifugation, preferably 3000 r/min, 5 min collect supernatant and marine alga sediment respectively；
Optional, can also include continuing to add absolute ethyl alcohol toward marine alga sediment, abovementioned steps are repeated, collect respectively
Supernatant and marine alga sediment；
The acquisition of marine alga natural antioxidant：The supernatant be concentrated under reduced pressure into it is dry, it is vacuum dried to obtain marine alga natural
Antioxidant product.
The marine alga is at least one in sea-tangle, seaweed, undaria pinnitafida, sargassum fusifome.
The marine alga slag is the residue after marine alga extracts polysaccharide.
In the preparation of the marine alga slag dry powder, sieve 80-100 mesh.
In the biological enzymolysis broken wall of the marine alga slag dry powder, the seaweed meal is with the weight ratio of complex enzyme formulation
1000:5；The complex enzyme formulation is made up of pectase, cellulase, and it is 1 to preferably constitute ratio w/w:1；
Optional, the ratio of seaweed meal and complex enzyme formulation summation and deionized water is 1:The weight/volume of 15-25
Than；
Optional, deionized water is added after enzymolysis 5-8 hours under normal temperature.
In the organic solvent extraction, food-grade anhydrous ethanol is added, precipitation is centrifuged off after being extracted under normal temperature, take supernatant
Liquid；
Optional, the ratio of marine alga slag dry powder and food-grade anhydrous ethanol after broken wall is 1:The weight/volume of 5-10；
Optional, food-grade anhydrous ethanol is added after extraction 1-3 hours under normal temperature.
In the acquisition of the marine alga natural antioxidant, supernatant be concentrated under reduced pressure into it is dry, it is vacuum dried to obtain marine alga day
Right antioxidant product；
Optional, supernatant temperature concentrated under reduced pressure is 40-60 DEG C；
Optional, the vacuum drying temperature is 50-80 DEG C；
Optional, the vacuum drying time is 2-5 hours.
The marine alga natural antioxidative active extractive that methods described is obtained is paste, and the total phenol for containing is 5.2-13.7 matter
Amount %, total carotinoid is 3.6-8.4 mass %, and terpenoid is 0.5-1.3 mass %, and phytosterin compound is 0.9-2.6
Quality %.
Marine alga antioxidant of the present invention also obtained by protection either method.
The method of the present invention is simple to operate, environment-friendly high-efficiency, be suitable for large-scale production, adequately achieves the comprehensive of marine algae resource
Close and develop, improve economic benefit.
The present invention extracts marine alga natural polyphenoils using the marine alga slag extracted after polysaccharide, big due to alginic cell wall
Par-tial polysaccharide is extracted, the thickness reduction of cell membrane so that biology enzyme shell-broken effect is more preferably, intracellular to be filled containing active material
Divide release, the recovery rate of anti-oxidant ingredients is in 4 mass %-6.7 mass %.It is main that marine alga antioxidant extract now contains
Composition has seeweed polyphenol, carotenoid, terpene, sterols material.The anti-oxidation active substance obtained according to the method for the present invention
With patent application：A kind of method that enzyme process prepares brown alga fucosan and fucoxanthin（Application number：201310142757.X Shens
Please day：2013-04-24, publication date：2013-08-07）Compare, the active component for obtaining more fully, it is achieved thereby that resource
Maximally utilize.
The present invention uses marine alga processing enterprise to process the accessory substance-marine alga slag of algal polysaccharides for raw material prepares strong anti-oxidation
The natural of activity, realizes the comprehensive utilization of resource；Simple production process, efficiently, environmental protection is suitable to industrial metaplasia
Produce；The oxidation resistance of product is strong, green natural, and traditional chemical can be replaced to synthesize antioxidant use in the food industry.
This invention is degraded hard brown alga cell membrane by biology enzyme wall breaking technology, makes to be present in intracellular marine alga many
The antioxidant contents such as phenol, fucoxanthin are fully dissolved out, and realize the quick preparation of marine alga natural antioxidant.
The advantages of the present invention are：
1st, China's marine algae resource enriches, and by after the Polyose extraction in marine alga, marine alga slag is taken as inexpensively for marine alga processing enterprise
Feed or bio-feritlizer treatment, the polyphenoils such as polyphenol, fucoxanthin in marine alga do not obtain processing and utilization well,
The significant wastage of resource is caused, therefore industrialization development utilization is carried out to Natural Antioxidants in marine alga slag, with aobvious
The economic benefit and social benefit of work；
2nd, process is simple (Fig. 1 is shown in technological process) of the invention, environment-friendly high-efficiency, be suitable for scale operation, can be applied to
Industrialized production.
Embodiments of the invention, there is provided the method that natural is prepared using marine algas such as sea-tangles.In embodiment 1,
100 g kelp residue dry powder, finally give 6.5 g antioxidant extracts；In embodiment 2,100 g seaweed slag dry powder finally give
6.7 g antioxidant extracts；In embodiment 3,100 g opotism dish slag dry powder finally give 5.2 g antioxidant extracts；Implement
In example 4,100 g sargassum fusifome slag dry powder finally give 4.9 g antioxidant extracts.
When cellulase treatment broken wall is used to marine alga by step of the present invention, enable the antioxidant content in alginic cell abundant
Dissolution, so as to significantly improve extraction efficiency.Test with kelp residue dry powder as raw material, to pectase, cellulase and its composition
The shell-broken effect of compound formulation has carried out comparative study, and Kelp Powder (80 mesh) is fixed as 1000 with the ratio of biology enzyme：5 (w/
W), 5 h are hydrolyzed at room temperature, the optimum proportioning of biology enzyme is judged with the yield of ethanol extract, as a result as shown in table 1.Experiment knot
Fruit finds that after biology enzyme broken wall treatment, the yield of ethanol extract is all significantly improved, and pectase, fiber is used alone
Plain enzyme is neither as used two kinds of shell-broken effects of enzyme simultaneously.Meanwhile, experiment finds, the proportioning of pectase and cellulase is 1:
1（w/w）When shell-broken effect with other proportioning statistically there were significant differences.Meanwhile, by changing pectase and cellulose
The ratio of enzyme, finds the shell-broken effect of the complex enzyme of various proportionings, whether improves the proportion of pectase or cellulase,
Effect all presses 1 without pectase and cellulase:1（w/w）Shell-broken effect during proportioning is good, so pectase and cellulase are most
Excellent proportioning is 1:1（w/w）.
Influence table of the biology enzyme broken wall of table 1 to kelp residue ethanol extract yield
2nd, found through analysis of experiments, the anti-oxidation active substance content of the marine alga natural antioxidant that the present invention is obtained is high,
Suitable for the raw material of the industry such as food antioxidant and functional food and medicine.The constituent analysis of marine alga antioxidant extract is such as
Table 2：
The constituent analysis of the different marine alga antioxidant extracts of table 2
3rd, the marine alga natural antioxidant that the present invention is obtained with antioxidation activity it is high the characteristics of, can effective scavenger-cell
Interior free radical（Such as Fig. 2）.From figure 2 it can be seen that the marine alga natural antioxidant that the present invention is obtained is to intracellular free radicals
Maximal clearance in 42%-54%, the maximal clearance of vitamin E is 35%, illustrates the oxidation resistant cellular anti-oxidant of marine alga natural
Activity is better than vitamin E, can be used for the exploitation of food antioxidant and anti-oxidation functional food.
Brief description of the drawings
Fig. 1 is process chart of the invention.
Fig. 2 is effective removing figure of the marine alga natural antioxidant for obtaining of the invention to intracellular free radicals.
Specific embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from start to finish
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
It is exemplary to scheme the embodiment of description, it is intended to for explaining the present invention, and be not considered as limiting the invention.Embodiment
In unreceipted particular technique or condition person, according to the technology or condition described by document in the art or according to the description of product
Book is carried out.Agents useful for same or the unreceipted production firm person of instrument, be can by city available from conventional products.
Complex enzyme used by following examples is 1:1（w/w）Pectase and cellulase complex enzyme.
Embodiment 1：Sea-tangle prepares marine alga natural antioxidant
（1）The g of fresh sea-tangle 500 is taken, scissors is beaten after shredding with beater, adds 2.5 L deionized waters, is stirred；
80 DEG C of 1 h of extraction, are centrifuged using tubular type continuous centrifuge（3000 r/min, 5 min）, taking precipitate, acquisition
The g of marine alga slag 356.9；Marine alga slag drying, crushing, cross 80 mesh sieves and are made the g of marine alga slag dry powder 75.8；
（2）Take the above-mentioned g of kelp residue dry powder 50 fully to be mixed with 0.25 g complex enzymes, add the mL of deionized water 754, stirring
Uniformly.After 20 DEG C are reacted 5 h at room temperature, it is centrifuged using tubular type continuous centrifuge（3000 turns per minute, 5 minutes）, take sea-tangle
Slag.The step uses cellulase treatment broken wall to marine alga slag, makes the polyphenoils mass-energy such as polyphenol, fucoxanthin in marine alga abundant
Dissolution, so as to significantly improve extraction efficiency.Destruction of the biology enzyme to alginic cell wall is judged by the recovery rate of ethanol extract
Effect（It is shown in Table 1）.As can be seen from Table 1, the recovery rate of ethanol extract is 1.52 ± 0.36 g/100 g before ferment treatment, when pressing
After step 2 carries out ferment treatment to marine alga slag, the recovery rate of ethanol extract is significantly improved, when pectase and the proportioning of cellulase
It is 1:1 （w/w）When, the extraction rate reached of ethanol extract to 6.53 ± 0.13 g/100 g, effect is very notable；
（3）Above-mentioned kelp residue is put into baking oven in 6 h are dried at 80 DEG C, obtains the g of marine alga slag dry powder 45 after broken wall；
（4）The food-grade anhydrous ethanol of 225 mL is added toward the marine alga slag dry powder after the broken wall, 20 DEG C extract at room temperature
2 h, centrifugation（3000 turns per minute, 5 minutes）, supernatant and marine alga sediment are collected respectively；
（5）Repeat step 4, the supernatant that combining step 4 and step 5 are obtained；
（6）Above-mentioned supernatant is dry in being concentrated under reduced pressure at 60 DEG C, then using being vacuum dried in 70 DEG C of h of drying 3, obtains
To the g of marine alga natural antioxidant product 3.29.
Embodiment 2：Sargassum fusifome prepares marine alga natural antioxidant
（1）The g of sargassum fusifome 500 is taken, scissors is beaten after shredding with beater, adds 2.5 L deionized waters, is stirred.
80 DEG C of 1 h of extraction, are centrifuged using tubular type continuous centrifuge（3000 r/min, 5 min）, taking precipitate, acquisition
The g of marine alga slag 385.7；Marine alga slag drying, crushing, cross 80 mesh sieves and are made the g of marine alga slag dry powder 81.4；
（2）Take the above-mentioned g of marine alga slag dry powder 50 fully to be mixed with 0.25 g complex enzymes, add the mL of deionized water 1005, stir
Mix uniform.After 20 DEG C are reacted 5 h at room temperature, it is centrifuged using tubular type continuous centrifuge（3000 turns per minute, 5 minutes）, take marine alga
Slag；
（3）Above-mentioned marine alga slag is put into baking oven in 6 h are dried at 80 DEG C, obtains the g of marine alga slag dry powder 46 after broken wall；
（4）The food-grade anhydrous ethanol of 368 mL is added toward the marine alga slag dry powder after the broken wall, 20 DEG C extract at room temperature
2 h, centrifugation（3000 turns per minute, 5 minutes）, supernatant and marine alga sediment are collected respectively；
（5）Repeat step 4, the supernatant that combining step 4 and step 5 are obtained；
（6）Above-mentioned supernatant is dry in being concentrated under reduced pressure at 60 DEG C, then using being vacuum dried in 70 DEG C of h of drying 3, obtains
To the g of marine alga natural antioxidant product 2.47.
Embodiment 3：Undaria pinnitafida prepares marine alga natural antioxidant
（1）The g of fresh undaria pinnitafida 500 is taken, scissors is beaten after shredding with beater, adds 2.5 L deionized waters, is stirred；
80 DEG C of 1 h of extraction, are centrifuged using tubular type continuous centrifuge（3000 r/min, 5 min）, taking precipitate, acquisition
The g of marine alga slag 322.5；Marine alga slag drying, crushing, cross 80 mesh sieves and are made the g of marine alga slag dry powder 65.2；
（2）Take the above-mentioned g of marine alga slag dry powder 50 fully to be mixed with 0.25 g complex enzymes, add the mL of deionized water 1256, stir
Mix uniform.After 20 DEG C are reacted 5 h at room temperature, it is centrifuged using tubular type continuous centrifuge（3000 turns per minute, 5 minutes）, take marine alga
Slag；
（3）Above-mentioned marine alga slag is put into baking oven in 6 h are dried at 80 DEG C, obtains the g of marine alga slag dry powder 43 after broken wall；
（4）The food-grade anhydrous ethanol of 430 mL is added toward the marine alga slag dry powder after the broken wall, 20 DEG C extract at room temperature
2 h, centrifugation（3000 turns per minute, 5 minutes）, supernatant and marine alga sediment are collected respectively；
（5）Repeat step 4, the supernatant that combining step 4 and step 5 are obtained；
（6）Above-mentioned supernatant is dry in being concentrated under reduced pressure at 60 DEG C, then using being vacuum dried in 70 DEG C of h of drying 3, obtains
To the g of marine alga natural antioxidant product 2.66.
Embodiment 4：Seaweed prepares marine alga natural antioxidant
（1）The g of fresh laver 500 is taken, scissors is beaten after shredding with beater, adds 2.5 L deionized waters, is stirred；
80 DEG C of 1 h of extraction, are centrifuged using tubular type continuous centrifuge（3000 r/min, 5 min）, taking precipitate, acquisition
The g of marine alga slag 349.7；Marine alga slag drying, crushing, cross 80 mesh sieves and are made the g of marine alga slag dry powder 70.6；
（2）Take the above-mentioned g of marine alga slag dry powder 50 fully to be mixed with 0.25 g complex enzymes, add the mL of deionized water 1005, stir
Mix uniform.After 20 DEG C are reacted 5 h at room temperature, it is centrifuged using tubular type continuous centrifuge（3000 turns per minute, 5 minutes）, take marine alga
Slag；
（3）Above-mentioned marine alga slag is put into baking oven in 6 h are dried at 80 DEG C, obtains the g of marine alga slag dry powder 47 after broken wall；
（4）The food-grade anhydrous ethanol of 376 mL is added toward the marine alga slag dry powder after the broken wall, 20 DEG C extract at room temperature
2 h, centrifugation（3000 turns per minute, 5 minutes）, supernatant and marine alga sediment are collected respectively；
（5）Repeat step 4, the supernatant that combining step 4 and step 5 are obtained；
（6）Above-mentioned supernatant is dry in being concentrated under reduced pressure at 60 DEG C, then using being vacuum dried in 70 DEG C of h of drying 3, obtains
To the g of marine alga natural antioxidant product 3.38.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from principle of the invention and objective
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (9)
1. a kind of preparation method of marine alga natural antioxidant, it is characterised in that comprise the following steps,
The preparation of marine alga slag：New fresh seaweed is beaten to without obvious bulk thing, and by volume 1:5 add 80 DEG C of extractions after deionized water
1h, centrifugation, 3000r/min, 5min, taking precipitate obtains marine alga slag；
Optional, can also include continuing to add deionized water toward marine alga slag, abovementioned steps are repeated, collect marine alga slag；
The preparation of marine alga slag dry powder：Marine alga slag drying, crushing, sieving are made marine alga slag dry powder；
The biological enzymolysis broken wall of marine alga slag dry powder：Deionized water is added after the marine alga slag dry powder and complex enzyme formulation are mixed,
In certain hour is digested under normal temperature, it is centrifuged, 3000r/min, 5min, collects sediment, 80 DEG C dries 6h in an oven, are broken
Marine alga slag dry powder after wall；The marine alga slag dry powder is 1000 with the weight ratio of complex enzyme formulation:5；The complex enzyme formulation by
Pectase, cellulase composition；
Organic solvent is extracted：Added toward the marine alga slag dry powder after the broken wall and extract certain hour under absolute ethyl alcohol, normal temperature, from
The heart, 3000r/min, 5min collect supernatant and marine alga sediment respectively；
Optional, can also include continuing to add absolute ethyl alcohol toward marine alga sediment, abovementioned steps are repeated, supernatant is collected respectively
Liquid and marine alga sediment；
The acquisition of marine alga natural antioxidant：The supernatant be concentrated under reduced pressure into it is dry, it is vacuum dried to obtain marine alga natural antioxygen
Agent product.
2. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The marine alga is sea-tangle, purple
At least one in dish, undaria pinnitafida, sargassum fusifome.
3. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The marine alga slag is carried for marine alga
Take the residue after polysaccharide.
4. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The system of the marine alga slag dry powder
In standby, sieve 80-100 mesh.
5. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The marine alga slag dry powder with it is multiple
The ratio of synthase preparation summation and deionized water is 1:The weight/volume of 15-25；
Optional, deionized water is added after enzymolysis 5-8 hours under normal temperature.
6. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The complex enzyme formulation is by w/
W is 1:1 pectase, cellulase composition.
7. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The organic solvent extraction
In, food-grade anhydrous ethanol is added, precipitation is centrifuged off after being extracted under normal temperature, take supernatant；
Optional, the ratio of marine alga slag dry powder and food-grade anhydrous ethanol after broken wall is 1:The weight/volume of 5-10；
Optional, food-grade anhydrous ethanol is added after extraction 1-3 hours under normal temperature.
8. the preparation method of a kind of marine alga natural antioxidant of claim 1, it is characterised in that：The marine alga natural is anti-oxidant
In the acquisition of agent, supernatant be concentrated under reduced pressure into it is dry, it is vacuum dried to obtain marine alga natural antioxidant product；
Optional, supernatant temperature concentrated under reduced pressure is 40-60 DEG C；
Optional, the vacuum drying temperature is 50-80 DEG C；
Optional, the vacuum drying time is 2-5 hours.
9. the marine alga antioxidant obtained by claim 1-8 either method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206699.7A CN104001180B (en) | 2014-05-16 | 2014-05-16 | A kind of preparation method of marine alga natural antioxidant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206699.7A CN104001180B (en) | 2014-05-16 | 2014-05-16 | A kind of preparation method of marine alga natural antioxidant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104001180A CN104001180A (en) | 2014-08-27 |
| CN104001180B true CN104001180B (en) | 2017-07-11 |
Family
ID=51362263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410206699.7A Expired - Fee Related CN104001180B (en) | 2014-05-16 | 2014-05-16 | A kind of preparation method of marine alga natural antioxidant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104001180B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663928A (en) * | 2015-02-11 | 2015-06-03 | 河南工业大学 | Frying oil stabilizer and use method thereof |
| CN105878230A (en) * | 2016-05-07 | 2016-08-24 | 集美大学 | New application of fucoxanthin to eyesight protection |
| CN106974284A (en) * | 2017-03-06 | 2017-07-25 | 广东药科大学 | A kind of chlamydomonas ethanol extract and preparation method and application |
| CN106962931B (en) * | 2017-03-06 | 2020-11-27 | 广东药科大学 | Horseradish algae intracellular fluid and preparation method and application thereof |
| CN107156844B (en) * | 2017-05-25 | 2021-03-02 | 广东药科大学 | Preparation method and application of sphingomyelina crypthecogenica ethanol extract |
| CN109805375B (en) * | 2019-01-28 | 2022-05-17 | 集美大学 | Functional food for improving oxidation resistance of visual organs and preparation method thereof |
| CN113080145B (en) * | 2021-04-01 | 2022-09-16 | 广东省农业科学院蚕业与农产品加工研究所 | Method for recycling edible fungus by-products by using earthworms |
| CN115380918B (en) * | 2022-08-29 | 2024-01-26 | 福州大学 | Method for optimized extraction of kelp polyphenol by response surface method |
| CN116623312B (en) * | 2023-05-31 | 2024-02-02 | 芝诺(苏州)生物科技有限公司 | Dacron macrofiber containing 2' -fucosyllactose and seaweed extract and preparation method thereof |
| CN116555933B (en) * | 2023-05-31 | 2024-02-02 | 芝诺(苏州)生物科技有限公司 | ES macrobiological fiber containing 2' -fucosyllactose and seaweed extract and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232552A (en) * | 2013-04-24 | 2013-08-07 | 集美大学 | Method for preparing brown algae fucosan and fucoxanthin in enzymic way |
-
2014
- 2014-05-16 CN CN201410206699.7A patent/CN104001180B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232552A (en) * | 2013-04-24 | 2013-08-07 | 集美大学 | Method for preparing brown algae fucosan and fucoxanthin in enzymic way |
Non-Patent Citations (1)
| Title |
|---|
| 复合酶法提取海带岩藻黄素及其抗氧化活性分析;秦云 等;《食品科学》;20130107;第34卷(第16期);摘要及1.3节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104001180A (en) | 2014-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104001180B (en) | A kind of preparation method of marine alga natural antioxidant | |
| Beres et al. | Towards integral utilization of grape pomace from winemaking process: A review | |
| Shim et al. | Flaxseed (Linum usitatissimum L.) oil processing and selected products | |
| Ashokkumar et al. | Technological advances in the production of carotenoids and their applications–A critical review | |
| CN101812111A (en) | Comprehensive deep processing method for oil tea fruits | |
| CN104557648A (en) | Method for preparing beta-carotene | |
| CN104804880A (en) | Method for preparing natural detergent through microbial fermentation of sapindus peels | |
| Picot-Allain et al. | Assessing the bioactivity, cytotoxicity, and rheological properties of pectin recovered from citrus peels | |
| Talekar et al. | Rapid, enhanced and eco-friendly recovery of punicalagin from fresh waste pomegranate peels via aqueous ball milling | |
| Berenguer et al. | Current challenges in the sustainable valorisation of agri-food wastes: a review | |
| CN104003962B (en) | A kind of preparation method and its usage of fucoxanthin | |
| CN102511569B (en) | Production method for extracting allicin oil from garlic | |
| CN101787067B (en) | Method for extraction of ergosterol by mushroom scrap | |
| CN108929770A (en) | A method of utilizing enzyme assisted extraction soybean grease | |
| CN109418668A (en) | A kind of Rice Bran liquid and preparation method thereof | |
| CN102276455A (en) | Method for extracting effective ingredients from dry cinnamomum burmannii fruits | |
| CN108703278A (en) | A kind of preparation method of the full fruit fruit juice of navel orange | |
| Cecchi et al. | Biocascading: General Strategy for the Recovery of Valuable Substances from Food Waste | |
| CN111039841B (en) | Method for simultaneously extracting carotenoid and polyphenol from green leaf vegetables | |
| Muzaffar et al. | Fruit Processing Wastes and By-Products | |
| KR20150142958A (en) | A preparation method of fermented and puffed ginseng having high ginsenoside contents | |
| Prokopov | Utilization of by-products from fruit and vegetable processing: a review | |
| CN104814505B (en) | A kind of preparation method and applications of natural composite antioxidant | |
| CN109553698A (en) | A kind of pectin from lemon peel pre-treating method | |
| Pathak et al. | Fruit peel waste” Biorefinery” and sustainability issues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170711 Termination date: 20200516 |