CN104000845A - Acetic acid and nano zinc oxide combined synergistic bacteriostasic composition - Google Patents
Acetic acid and nano zinc oxide combined synergistic bacteriostasic composition Download PDFInfo
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- CN104000845A CN104000845A CN201410179502.5A CN201410179502A CN104000845A CN 104000845 A CN104000845 A CN 104000845A CN 201410179502 A CN201410179502 A CN 201410179502A CN 104000845 A CN104000845 A CN 104000845A
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- acetic acid
- bacteriostasic
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an acetic acid and nano zinc oxide combined synergistic bacteriostasic composition, and belongs to the field of medical science. Combined use of two bacteriostasic agents of acetic acid and nano zinc oxide in the bacteriostasic composition plays a combined synergistic bacteriostasic effect on inhibition of escherichia coli O157, bacillus cereus, salmonella and staphylococcus aureus. Compared with the prior art, The acetic acid and nano zinc oxide bacteriostasic composition not only solves the problems that corrosiveness exists, steam has an irritation effect to eyes and noses and bacteria are easy to varied to generate drug resistance to acids when a single acetic acid is used for bacteriostasis and has high concentration, but also solves nano zinc oxide is difficult to dissolve in water in a conventional bacteriostasic method, and allows the biocompatibility of the two bacteriostasic agents to be improved so as to be more widely applied.
Description
Technical field
The invention belongs to field of medicaments, relate in particular to bacteria inhibiting composition.
Background technology
Acetic acid, is also acetic acid, glacial acetic acid, chemical formula CH
3cOOH, is a kind of organic acid, can obtain by the several different methods such as aerobic fermentation, anaerobic fermentation and chemical industry synthesize preparation.The acetic acid of variable concentrations infects to treat various superficial skin fungus medically conventional, the treatment of lavation wound surface and clavus, wart.Modern experimentation shows that acetic acid has the effect that suppresses pathogenic microorganism.Acetic acid is in the time making medicine antibacterial, and ampoule is large, and concentration is high, and life-time service affects health.
Summary of the invention
Large for acetic acid bacteria inhibiting composition ampoule, concentration height and life-time service affect health problem, the inventor is through great many of experiments, find that nano zine oxide can effectively strengthen acetic acid solution bacteriostasis property, reduce the consumption of acetic acid, reduce the concentration of acetic acid, and adopt the bacteria inhibiting composition of two kinds of low dosages, reduce the probability of microorganisms drug resistance, more safe and efficient.The object of the present invention is to provide a kind of acetic acid and the antibacterial complex of nano zine oxide potentiation, reduce and reduce acetic acid antibacterial use dosage and concentration and the toxicity of reduction medicine to human body once.
The invention provides following technical scheme:
Associating acetic acid and nano zine oxide potentiation bacteria inhibiting composition, contain acetic acid, nano zine oxide.
Described bacterium is Escherichia coli O 157, and acetic acid solution concentration is 0.0087mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
Described bacterium is Bacillus cereus, and acetic acid solution concentration is 0.0059mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
Described bacterium is Salmonella, and acetic acid solution concentration is 0.0108mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
Described bacterium is staphylococcus aureus, and acetic acid solution concentration is 0.0145mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
The invention still further relates to described bacteria inhibiting composition and suppress the application in Escherichia coli O 157, Bacillus cereus, Salmonella, staphylococcus aureus medicine in preparation.
The invention has the beneficial effects as follows: nano zine oxide can effectively strengthen acetic acid solution bacteriostasis property, reduce the consumption of acetic acid, reduce the concentration of acetic acid, and adopt the bacteria inhibiting composition of two kinds of low dosages, reduce the probability of microorganisms drug resistance, more safe and efficient.
Detailed description of the invention
Embodiment 1
1, the preparation of low concentration acetic acid diluent comprises the steps: to get analytical pure acetic acid as original liquid, with the distilled water serial dilution after sterilizing, obtains successively variable concentrations acetic acid solution.The original concentration of acetic acid is 17.4mol/L.
2, the preparation of nano zine oxide colloid solution: the nano zine oxide 0.01g that gets the production of Shanghai Aladdin company is dissolved in the distilled water of 1mL, and concussion mixes the nano zine oxide colloid solution that obtains 10mg/mL.
3, the synergy bacteriostasis of acetic acid and nano zine oxide
The cultivation of a antibacterial
Single bacterium colony of the Escherichia coli O 157 on picking LB agar culture medium is in 10mL LB broth bouillon, be placed in 37 DEG C of incubators and cultivate 12 h, be inoculated in the LB broth bouillon of 5 mL by 1% inoculum concentration again, cultivate after 5h for 37 DEG C, get 2 gradients of 1 mL above-mentioned bacterium liquid 1xPBS water serial dilution, final bacterium liquid is approximately 10
6cFU/mL.
B acetic acid and the nano zine oxide synergism on bacteriostasis property
Get respectively the above-mentioned bacterium liquid having diluted of 900 μ L in 1.5mL centrifuge tube, add respectively the antibacterial that 100 μ L are different (specifically in table one).Be placed at 37 DEG C and cultivate 5h, every group parallel 3 times.
C plate count
By each group of 4 gradients of 1xPBS serial dilution for liquid to be measured, 10
-2, 10
-3, 10
-4in each gradient, take out 10 μ L on LB flat board.After flat board dries up, be inverted and cultivate 12h at 37 DEG C, count after growing bacterium colony, taking 30-300 colony-forming units (CFU) as effective count range.
Experimental result is as table one:
Table one
| Numbering | Antibacterial (being 100 μ L) | Add the clump count after antibacterial |
| 1 | The acetic acid of 0.174mol/L | 69x10 6 |
| 2 | The mixed liquor of the acetic acid volume ratio 1:1 of 10mg/mL nano zine oxide colloid solution and 0.174mol/L | 31x10 5 |
| 3 | The acetic acid of 0.145mol/L | 103x10 7 |
| 4 | The acetic acid volume ratio 1:1 mixed liquor of 10mg/mL nano zine oxide colloid solution and 0.145mol/L | 89x10 6 |
| 5 | The acetic acid of 0.118mol/L | 30x10 8 |
| 6 | The acetic acid volume ratio 1:1 mixed liquor of 10mg/mL nano zine oxide colloid solution and 0.118mol/L | 33x10 7 |
| 7 | Concentration is the nano zine oxide colloid solution of 10 mg/mL | 67x10 8 |
| 8 | 1xPBS | 92x10 8 |
Experiment shows: antibacterial complex provided by the invention not only has the nano zine oxide of promotion dissolution, simultaneously by reducing the Mlc of acetic acid, greatly reduce the stimulation to human body, and share of two kinds of antibacterial substances played collaborative effect to suppressing Escherichia coli O 157.The mixed liquor fungistatic effect of the acetic acid volume ratio 1:1 of 10mg/mL nano zine oxide colloid solution and 0.174mol/L is best, and in bacterium liquid, both actual concentrations are: acetic acid concentration is 0.0087mol/L, and nano oxidized zinc concentration is 0.5mg/mL.
Embodiment 2
1, the preparation of low concentration acetic acid diluent comprises the steps: same embodiment 1.
2, the preparation of nano zine oxide colloid solution: with embodiment 1.
3, the synergy bacteriostasis of acetic acid and nano zine oxide
The cultivation of a antibacterial
Single bacterium colony of the Bacillus cereus on picking LB agar culture medium is in 10mL LB broth bouillon, be placed in 37 DEG C of incubators and cultivate 12 h, be inoculated in the LB broth bouillon of 5 mL by 1% inoculum concentration again, cultivate after 5h for 37 DEG C, get 2 gradients of 1 mL above-mentioned bacterium liquid 1xPBS water serial dilution, final bacterium liquid is approximately 10
6cFU/mL.
B acetic acid and the nano zine oxide synergism on bacteriostasis property
Get respectively the above-mentioned bacterium liquid having diluted of 900 μ L in 1.5mL centrifuge tube, add respectively the antibacterial that 100 μ L are different (specifically in table two).Be placed at 37 DEG C and cultivate 5h, every group parallel 3 times.
C plate count
By each group of 4 gradients of 1xPBS serial dilution for liquid to be measured, 10
-2, 10
-3, 10
-4each 10 μ L that take out are on LB flat board.After flat board dries up, be inverted and cultivate 12h at 37 DEG C, count after growing bacterium colony, taking 30-300 colony-forming units (CFU) as effective count range.
Experimental result is as follows:
Table two
| Numbering | Antibacterial (being 100 μ L) | Add the clump count after antibacterial |
| 1 | The acetic acid of 0.118mol/L | 124x10 6 |
| 2 | The mixed liquor of the acetic acid volume ratio 1:1 of 10 mg/mL nano zine oxide colloid solution and 0.118mol/L | 38x10 6 |
| 3 | The acetic acid of 0.10875mol/L | 121x10 7 |
| 4 | The acetic acid volume ratio 1:1 mixed liquor of 10 mg/mL nano zine oxide colloid solution and 0.10875mol/L | 46x10 7 |
| 5 | The acetic acid of 0.087mol/L | 62x10 8 |
| 6 | The acetic acid volume ratio 1:1 mixed liquor of 10 mg/mL nano zine oxide colloid solution and 0.087mol/L | 34x10 8 |
| 7 | Concentration is the nano zine oxide colloid solution of 10 mg/mL | 102x10 8 |
| 8 | 1xPBS | 133x10 8 |
Experiment shows: bacteria inhibiting composition provided by the invention not only has the nano zine oxide of promotion dissolution, simultaneously by reducing the Mlc of acetic acid, greatly reduce the stimulation to human body, and share of two kinds of antibacterial substances played collaborative effect to suppressing Bacillus cereus.In numbering 2, acetic acid concentration is 0.0059mol/L, and nano oxidized zinc concentration is 0.5mg/mL fungistatic effect the best.
Embodiment 3
1, the preparation of low concentration acetic acid diluent comprises the steps: same embodiment 1.
2, the preparation of nano zine oxide colloid solution: with embodiment 1.
3, the synergy bacteriostasis of acetic acid and nano zine oxide
The cultivation of a antibacterial
Single bacterium colony of the Salmonella on picking LB agar culture medium is in 10mL LB broth bouillon, be placed in 37 DEG C of incubators and cultivate 12 h, be inoculated in the LB broth bouillon of 5 mL by 1% inoculum concentration again, cultivate after 5h for 37 DEG C, get 2 gradients of 1 mL above-mentioned bacterium liquid 1xPBS water serial dilution, final bacterium liquid is approximately 10
5cFU/mL.
B acetic acid and the nano zine oxide synergism on bacteriostasis property
Get respectively the above-mentioned bacterium liquid having diluted of 900 μ L in 1.5mL centrifuge tube, add respectively the antibacterial that 100 μ L are different (specifically in table three).Be placed at 37 DEG C and cultivate 5h, every group parallel 3 times
C plate count
By each group of 3 gradients of 1xPBS serial dilution for liquid to be measured, 10
-1, 10
-2, 10
-3each 10 μ L that take out are on LB flat board.After flat board dries up, be inverted and cultivate 12h at 37 DEG C, count after growing bacterium colony, taking 30-300 colony-forming units (CFU) as effective count range.
Experimental result is as table three:
| Numbering | Antibacterial (being 100 μ L) | Add the clump count after antibacterial |
| 1 | The acetic acid of 0.2175mol/L | 92x10 4 |
| 2 | The mixed liquor of the acetic acid volume ratio 1:1 of the nano zine oxide colloid solution of 10 mg/mL and 0.2175mol/L | 143x10 3 |
| 3 | The acetic acid of 0.174mol/L | 132x10 5 |
| 4 | The acetic acid volume ratio 1:1 mixed liquor of the nano zine oxide colloid solution of 10 mg/mL and 0.174mol/L | 46x10 5 |
| 5 | The acetic acid of 0.145mol/L | 87x10 6 |
| 6 | The acetic acid volume ratio 1:1 mixed liquor of the nano zine oxide colloid solution of 10 mg/mL and 0.145mol/L | 34x10 6 |
| 7 | Concentration is the nano zine oxide colloid solution of 10 mg/mL | 97x10 7 |
| 8 | 1xPBS | 133x10 7 |
Experiment shows: bacteria inhibiting composition provided by the invention not only has the nano zine oxide of promotion dissolution, simultaneously by reducing the Mlc of acetic acid, greatly reduce the stimulation to human body, and share of two kinds of antibacterial substances played collaborative effect to suppressing Salmonella.In numbering 2, acetic acid concentration is 0.0108mol/L, and nano oxidized zinc concentration is 0.5mg/mL, fungistatic effect the best.
Embodiment 4
1, the preparation of low concentration acetic acid diluent comprises the steps: same embodiment 1.
2, the preparation of nano zine oxide colloid solution: with embodiment 1.
3, the synergy bacteriostasis of acetic acid and nano zine oxide
The cultivation of a antibacterial
Single bacterium colony of staphylococcus aureus on picking LB agar culture medium is in 10mL LB broth bouillon, be placed in 37 DEG C of incubators and cultivate 12 h, be inoculated in the LB broth bouillon of 5 mL by 1% inoculum concentration again, cultivate after 5h for 37 DEG C, get 2 gradients of 1 mL above-mentioned bacterium liquid 1xPBS water serial dilution, final bacterium liquid is approximately 10
6cFU/mL.
B acetic acid and the nano zine oxide synergism on bacteriostasis property
Get respectively the above-mentioned bacterium liquid having diluted of 900 μ L in 1.5mL centrifuge tube, add respectively the antibacterial that 100 μ L are different (specifically in table four).Be placed at 37 DEG C and cultivate 5h, every group parallel 3 times
C plate count
By each group of 4 gradients of 1xPBS serial dilution for liquid to be measured, 10
-2, 10
-3, 10
-4each middle taking-up 10 μ L are on LB flat board.After flat board dries up, be inverted and cultivate 12h at 37 DEG C, count after growing bacterium colony, taking 30-300 colony-forming units (CFU) as effective count range.
Experimental result is as table four:
| Numbering | Antibacterial (being 100 μ L) | Add the clump count after antibacterial |
| 1 | The acetic acid of 0.29mol/L | 67x10 5 |
| 2 | The mixed liquor of the acetic acid volume ratio 1:1 of the nano zine oxide colloid solution of 10 mg/mL and 0.29mol/L | 172x10 3 |
| 3 | The acetic acid of 0.2175mol/L | 59x10 7 |
| 4 | The acetic acid volume ratio 1:1 mixed liquor of the nano zine oxide colloid solution of 10 mg/mL and 0.21755mol/L | 46x10 6 |
| 5 | The acetic acid of 0.174mol/L | 43x10 8 |
| 6 | The acetic acid volume ratio 1:1 mixed liquor of the nano zine oxide colloid solution of 10 mg/mL and 0.174mol/L | 121x10 7 |
| 7 | Concentration is the nano zine oxide colloid solution of 10 mg/mL | 93x10 8 |
| 8 | 1xPBS | 144x10 8 |
Experiment shows: bacteria inhibiting composition provided by the invention not only has the nano zine oxide of promotion dissolution, simultaneously by reducing the Mlc of acetic acid, greatly reduce the stimulation to human body, and share of two kinds of antibacterial substances played collaborative effect to suppressing staphylococcus aureus.In numbering 2, acetic acid concentration is 0.0145mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and fungistatic effect is best.
Claims (6)
1. associating acetic acid and nano zine oxide potentiation bacteria inhibiting composition, is characterized in that containing acetic acid, nano zine oxide.
2. bacteria inhibiting composition as claimed in claim 1, is characterized in that described bacterium is Escherichia coli O 157, and acetic acid concentration is 0.0087mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
3. the bacteria inhibiting composition as described in claim 1, is characterized in that described bacterium is Bacillus cereus, and acetic acid concentration is 0.0059mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
4. the bacteria inhibiting composition as described in claim 1, is characterized in that described bacterium is Salmonella, and acetic acid concentration is 0.0108mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
5. the bacteria inhibiting composition as described in claim 1, is characterized in that described bacterium is staphylococcus aureus, and acetic acid concentration is 0.0145mol/L, and nano oxidized zinc concentration is 0.5mg/mL, and nano granular of zinc oxide particle diameter is 200nm.
6. bacteria inhibiting composition claimed in claim 1 suppresses the application in Escherichia coli O 157, Bacillus cereus, Salmonella, staphylococcus aureus medicine in preparation.
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|---|---|---|---|---|
| US20090156994A1 (en) * | 2007-12-13 | 2009-06-18 | Ricardo Levisman | Pharmaceutical compound to prevent and treat focal tissular lesions and infections, method and applicators |
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| US20090156994A1 (en) * | 2007-12-13 | 2009-06-18 | Ricardo Levisman | Pharmaceutical compound to prevent and treat focal tissular lesions and infections, method and applicators |
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Application publication date: 20140827 |