CN103989030A - Method for ultrasonic-assisted detoxification of cotton seed meal - Google Patents
Method for ultrasonic-assisted detoxification of cotton seed meal Download PDFInfo
- Publication number
- CN103989030A CN103989030A CN201410206261.9A CN201410206261A CN103989030A CN 103989030 A CN103989030 A CN 103989030A CN 201410206261 A CN201410206261 A CN 201410206261A CN 103989030 A CN103989030 A CN 103989030A
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- China
- Prior art keywords
- detoxification
- ultrasonic
- cake dregs
- cotton cake
- cotton seed
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000001784 detoxification Methods 0.000 title claims abstract description 25
- 235000012054 meals Nutrition 0.000 title abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 69
- 239000007788 liquid Substances 0.000 claims description 2
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 abstract description 34
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 abstract description 17
- 229930000755 gossypol Natural products 0.000 abstract description 17
- 229950005277 gossypol Drugs 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract description 2
- 239000012527 feed solution Substances 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 235000012343 cottonseed oil Nutrition 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000000843 powder Substances 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 235000021251 pulses Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a method for ultrasonic-assisted detoxification of cotton seed meal, belonging to the technical field of food processing. The method comprises the following steps: (1) crushing the cotton seed meal and sieving; (2) adding a sodium hydroxide solution according to a certain feed-solution ratio; (3) processing with ultrasonic waves; and (4) drying a processed sample in an oven, and determining the content of free gossypol in the cotton seed meal. According to the method, after the cotton seed meal is subjected to ultrasonic wave processing in a detoxification process, the detoxification effect is remarkably improved.
Description
Technical field
The invention belongs to food processing technology field, particularly the method for the auxiliary cotton cake dregs detoxification of a kind of ultrasonic wave.
Background technology
The seed of cotton is called cottonseed, is one of important oil crops of China, and annual production has exceeded 1,100 ten thousand tons.Cottonseed can produce 12～18% the accessory substance such as cottonseed oil and the cotton cake dregs of 45% left and right through mechanical expression or organic solvent lixiviate etc.In cotton cake dregs, crude protein content is more than 35%, and wherein, except lysine content is lower, amino acid composition rationally, is the second-biggest-in-the-world plant protein material that is only second to dregs of beans.According to statistics, within 2012, China's Cottonseed Meal output has exceeded 6,000,000 tons, the output whole world first.
Gossypol is poisonous and harmful substance main in cotton cake dregs, gossypol comprises bound gossoypol and free gossypol, the toxicity of its Free Gossypol is larger, can with enzyme or other protein bound, destroy its activity, reduce protein digestibility, even make the histoorgan generation pathological change of animal, thereby cause growth of animal slow, poisoning and dead, thereby greatly limited the application of cotton cake dregs in animal feed and food industry.
The content of the edible cottonseed protein goods Free Gossypol of FAO's suggestion must not exceed 400 mg/kg; The forage health standard GB 13078-2001 of the country clearly allowance of the mixed feed Free Gossypol of regulation broiler chicken, laying hen and growing and fattening pigs should be no more than respectively 100,20 and 60 mg/kg.At present, the removal methods of cotton cake dregs Free Gossypol has wet heat treatment method, chemical detoxification method (to comprise FeSO both at home and abroad
4method, alkali treatment method, Urea treatment method, H
2o
2oxidizing process etc.), microbe fermentation method etc.Wherein wet heat treatment method is traditional treatment process, generally adopts boiling or the detoxification of Steam Heating pressure method.This method detoxification efficiency is unstable, and water consumption power consumption, and heating-up temperature is high to be destroyed seriously cotton cake dregs nutritional labeling.Microbe fermentation method removes cotton cake dregs Free Gossypol complicated operation, and the cycle is long.FeSO in chemical detoxication method
4and NaOH facture effect is better, and simple to operate, but FeSO
4color sample blackout after treatment, affects subsequent applications; Adopt NaOH to process detoxification to cotton cake dregs, along with the increase of NaOH concentration, detoxification efficiency is become better and better, but the color of cotton cake dregs after treatment is also more and more darker, and grouts local flavor variation.
In recent years, ultrasonic technology is more and more extensive in the application of food, medicine, chemical field, as active material extraction, sterilization, protein modified and albumen enzymatic hydrolysis etc.But about article or the patent of utilizing the auxiliary cotton cake dregs detoxification of ultrasonic technology there is not yet report.
Summary of the invention
Order of the present invention be exactly when overcoming cotton cake dregs and to adopt NaOH detoxification NaOH concentration used higher, affect the deficiency of grouts local flavor, provide a kind of ultrasonic wave to assist the method for cotton cake dregs detoxification.
The technical solution used in the present invention is: (1) pulverizes cotton cake dregs, crosses 40 mesh sieves, makes cotton cake dregs powder; (2) ultrasonic wave processing: taking cotton cake dregs powder as raw material, in solid-liquid ratio 1:5(g/mL) ratio to add concentration be 0.05%～0.2% sodium hydroxide solution; Adopt the pulse ultrasonic wave of power 200 W～800 W to process 5 min～20 min, ultrasonic intermittence, ratio was 1 s/2 s～3 s/2 s; The baking oven that rear ultrasonic wave processing sample is placed in to 55 DEG C of temperature is dried to moisture and is no more than 8%, can obtain the cotton cake dregs after detoxification.
The present invention adopts the auxiliary NaOH of ultrasonic wave to cotton cake dregs detoxification, and the cotton cake dregs Free Gossypol content after detoxification is measured.Through ultrasonic wave, processing can reduce the working concentration of NaOH greatly, has improved the impact of the simple cotton cake dregs local flavor variation causing with NaOH detoxification, has improved detoxification efficiency.
Detailed description of the invention
In the present invention, weigh detoxification efficiency with cotton cake dregs Free Gossypol content after detoxification, the mensuration of sample Free Gossypol content adopts GB 13086-91 method.
Reference examples 1
(1) cotton cake dregs is pulverized, crossed 40 mesh sieves, make cotton cake dregs powder; (2) take cotton cake dregs 5 g, adding 25 mL concentration is 0.05% sodium hydroxide solution, stirs to be placed in 55 DEG C of baking ovens of temperature to be dried 12 hours; (3) dried cotton cake dregs is pulverized with mortar, measure free gossypol content according to GB 13086-91 method.In table 1.
Reference examples 2
Test processing procedure is with reference examples 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.1%.In table 1.
Reference examples 3
Test processing procedure is with reference examples 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.15%.In table 1.
Reference examples 4
Test processing procedure is with reference examples 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.2%.In table 1.
Embodiment 1
(1) cotton cake dregs is pulverized, crossed 40 mesh sieves, make cotton cake dregs powder; (2) take cotton cake dregs 5 g, add the sodium hydroxide solution of 25 mL concentration 0.05%, adopt the pulse ultrasonic wave of power 200 W to process 5 min after stirring, ultrasonic intermittence, ratio was 1 s/2 s.After processing, sample is positioned in 55 DEG C of baking ovens of temperature and is dried 12 hours; (3) dried cotton cake dregs is pulverized with mortar, measure free gossypol content according to GB 13086-91 method.In table 1.
Embodiment 2
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.2%, and ultrasonic power is 800 W, ultrasonic time 20 min, and ultrasonic intermittence, ratio was 3 s/2 s.In table 1.
Embodiment 3
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.1%, and ultrasonic power is 400 W, ultrasonic time 9 min, and ultrasonic intermittence, ratio was 2 s/2 s.In table 1.
Embodiment 4
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.05%, and ultrasonic power is 600 W, ultrasonic time 12 min, and ultrasonic intermittence, ratio was 2 s/2 s.In table 1.
Embodiment 5
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.15%, and ultrasonic power is 800 W, ultrasonic time 6 min, and ultrasonic intermittence, ratio was 2 s/2 s.In table 1.
Embodiment 6
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.15%, and ultrasonic power is 400 W, ultrasonic time 12 min, and ultrasonic intermittence, ratio was 3 s/2 s.In table 1.
Embodiment 7
Test processing procedure is with embodiment 1, and its difference is that wherein concentration of sodium hydroxide solution is 0.15%, and ultrasonic power is 600 W, ultrasonic time 9 min, and ultrasonic intermittence, ratio was 1 s/2 s.In table 1.
The different embodiment cotton cake dregs of table 1 detoxification efficiency
| Processing mode | Free gossypol removal efficiency (%) |
| Reference examples 1 | 60.65 |
| Reference examples 2 | 61.57 |
| Reference examples 3 | 62.32 |
| Reference examples 4 | 63.05 |
| Embodiment 1 | 72.74 |
| Embodiment 2 | 93.98 |
| Embodiment 3 | 70.12 |
| Embodiment 4 | 81.69 |
| Embodiment 5 | 78.92 |
| Embodiment 6 | 85.30 |
| Embodiment 7 | 73.37 |
After measured, the content of raw material cotton cake dregs Free Gossypol is 830 mg/kg.
As can be seen from the above table, cotton cake dregs adds and is put in heat drying in baking oven after a certain amount of sodium hydroxide solution and has certain effect that removes free gossypol, and after applying ultrasonic wave and processing, cotton cake dregs detoxification efficiency obviously improves.
Claims (1)
1. a method for the auxiliary cotton cake dregs detoxification of ultrasonic wave, is characterized in that carrying out according to following step: (1) by cotton cake dregs according to solid-liquid ratio 1:5(g/mL) ratio to add concentration be 0.05%～0.2% sodium hydroxide solution; (2) adopt the pulse ultrasonic wave of power 200 W～800 W to process 5 min～20 min, ultrasonic intermittence, ratio was 1 s/2 s～3 s/2 s; (3) baking oven that rear ultrasonic wave processing sample is placed in to 55 DEG C of temperature is dried to moisture and is no more than 8%, can obtain the cotton cake dregs after detoxification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206261.9A CN103989030A (en) | 2014-05-16 | 2014-05-16 | Method for ultrasonic-assisted detoxification of cotton seed meal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206261.9A CN103989030A (en) | 2014-05-16 | 2014-05-16 | Method for ultrasonic-assisted detoxification of cotton seed meal |
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| Publication Number | Publication Date |
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| CN103989030A true CN103989030A (en) | 2014-08-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410206261.9A Pending CN103989030A (en) | 2014-05-16 | 2014-05-16 | Method for ultrasonic-assisted detoxification of cotton seed meal |
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| Country | Link |
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| CN (1) | CN103989030A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905003A (en) * | 2015-06-01 | 2015-09-16 | 江苏大学 | Method for detoxifying cottonseed meal |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016235A (en) * | 2007-02-12 | 2007-08-15 | 西安交通大学 | Method of extracting gossypol acetate from cotton seed oil niger |
| CN101253929A (en) * | 2008-04-14 | 2008-09-03 | 华中农业大学 | Quick high-efficient cotton cake dregs gossypol-removing method |
| CN102178030A (en) * | 2010-07-06 | 2011-09-14 | 新疆农业大学 | Economic and efficient method for removing gossypol from cotton by-products |
-
2014
- 2014-05-16 CN CN201410206261.9A patent/CN103989030A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016235A (en) * | 2007-02-12 | 2007-08-15 | 西安交通大学 | Method of extracting gossypol acetate from cotton seed oil niger |
| CN101253929A (en) * | 2008-04-14 | 2008-09-03 | 华中农业大学 | Quick high-efficient cotton cake dregs gossypol-removing method |
| CN102178030A (en) * | 2010-07-06 | 2011-09-14 | 新疆农业大学 | Economic and efficient method for removing gossypol from cotton by-products |
Non-Patent Citations (4)
| Title |
|---|
| 严伟等: "超声波协助提取技术", 《化工进展》, vol. 21, no. 9, 31 December 2002 (2002-12-31) * |
| 杨冲等: "棉籽粕中棉酚的超声辅助脱除研究", 《中国粮油学报》, vol. 26, no. 1, 31 January 2011 (2011-01-31) * |
| 贾光锋等: "基于超声波协同效应提取棉油泥中棉酚", 《粮食与饲料工业》, no. 12, 31 December 2011 (2011-12-31) * |
| 赛买提艾买提等: "五种不同方法对棉副产品棉酚脱毒效果的比较研究", 《饲料工业》, vol. 29, no. 1, 31 December 2008 (2008-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905003A (en) * | 2015-06-01 | 2015-09-16 | 江苏大学 | Method for detoxifying cottonseed meal |
| CN104905003B (en) * | 2015-06-01 | 2018-06-26 | 江苏大学 | A kind of method of cotton cake dregs detoxification |
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Application publication date: 20140820 |