CN103981277A - Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria - Google Patents
Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria Download PDFInfo
- Publication number
- CN103981277A CN103981277A CN201410249530.XA CN201410249530A CN103981277A CN 103981277 A CN103981277 A CN 103981277A CN 201410249530 A CN201410249530 A CN 201410249530A CN 103981277 A CN103981277 A CN 103981277A
- Authority
- CN
- China
- Prior art keywords
- oxidizing bacteria
- propane
- methane
- anomaly
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The invention discloses an oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria, which comprises the following steps: after homogenizing a soil sample, extracting sample DNA (deoxyribonucleic acid), and quantifying propane oxidizing bacteria and methane oxidizing bacteria by a fluorescence quantitative PCR (polymerase chain reaction) technique; determining anomaly thresholds of the propane oxidizing bacteria and methane oxidizing bacteria, establishing a microbiological anomaly reference system for verification zone oil-gas microbiological exploration, drawing isoline of the propane oxidizing bacteria, and carrying out microbiological anomaly zoning; and marking the gas-cap reservoir and pure reservoir in the anomaly zone according to the content of the methane oxidizing bacteria. According to the method, the anomaly of light hydrocarbon oxidizing bacteria is utilized to perform oil-gas exploration; in the precision detection aspect, the quantity of the light hydrocarbon oxidizing bacteria in the sample is directly detected to avoid the artificial interference in the traditional culture method; and the detection quantity is higher than that of the traditional culture method by 1-2 orders of magnitude, the time is shortened to 12 hours from 14-21 days in the traditional culture method, and the method can detect 96 samples at one time, thereby effectively enhancing the precision and detection efficiency of microbial oil-gas exploration.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of employing Protocols in Molecular Biology, and be applied to Microbial prospecting of oil and gas, effectively avoid earth's surface to pollute or the biogenic Microbial Prospecting of Oil and Gas method of near surface.
Background technology
Oil and natural gas is the basic energy resource that concerns national economy.Through 3-d seismic exploration for many years, the hydrocarbon-bearing pool resource that China's great majority are easy to find has been verified and has developed.Less, the non-structure type of scale and hidden hydrocarbon-bearing pool resource become the primary goal of current exploration.But these hydrocarbon resourceses are used, the means prospecting prime cost of earthquakes is too high and data analysis is difficult.
Microbial prospecting technology of oil & is a branch of near surface geochemical exploration, has the features such as direct, effective, cost is low.It can be used as a kind of independent technique, not having geology or seismic data area to carry out advanced exploration, determines prospective area, carries out reservoir properties and Reserves Evaluation; Also can combine with 3-D seismics and chemistry exploration, determine more accurately and expand limit increasing the storage by well location, find out the hydrocarbon-bearing pool of undiscovered oil and gas bearing trap and leakage, increase success ratio, reduce prospecting prime cost and risk.
The know-why of Microbial Prospecting of Oil and Gas is: be imbedded in the hydrocarbon-bearing pool of deep under ground, under various dynamic actions, the lighter hydrocarbons gas (being mainly methane, ethane, propane, butane) of hydrocarbon-bearing pool continues to earth's surface diffusion and migration by micro-seepage, obligate microorganism in soil is taking lighter hydrocarbons gas as its unique energy derive, in surface soil directly over oil reservoir, grow very much and form microbiological anomaly, by detecting the existence of the measurable hydrocarbon-bearing pool of underliing of microbiological anomaly.Micro-seepage of lighter hydrocarbons gas has three features: the one, and vertical property, migratory direction when light hydrocarbon microseepage is vertical generally, so the scope of microbiological anomaly is roughly corresponding to the border of subterranean oil gas reservoir, and the intensity of microbiological anomaly is corresponding with the original content of hydrocarbon-bearing pool; The 2nd, ubiquity, i.e. all there is micro-seepage of lighter hydrocarbons gas in most hydrocarbon-bearing pool, so microorganism detection method is suitable for the exploration of most of oil gas reservoir; The 3rd, dynamic, along with the continually developing of hydrocarbon-bearing pool, light hydrocarbon microseepage intensity can corresponding reduction, and this dynamic change can characterize the changes in distribution of Remaining Oil And Gas in oil-gas field development.
Microbial prospecting technology of oil & was founded by USSR (Union of Soviet Socialist Republics) scientist in nineteen thirty-seven, and subsequently, the U.S. and Germany have carried out a large amount of research work, and India has also carried out correlative study in recent years.China starts from mid-term the 1950's at this area research, and after the nineties, this technology is paid attention to again.At present, the microbial prospecting technology of oil & of being used widely is mainly Microbial prospecting of oil and gas (the Microbial Prospection for Oil and Gas of German doctor's Wagner research and development, MPOG) microorganism oil-gas prospecting (Microbial Oil Survey Technique, the MOST) technology of technology and U.S. doctor Hitzman research and development.These two kinds of technology are culture methods to microorganism detection method.The indicative bacterial classification relating generally to comprises methane-oxidizing bacteria, ethane oxidation bacterium, oxidation of propane bacterium and butane oxidation bacterium etc.But it is reported, the microorganism in soil more than 99.7% is all not educable, so culture method can not be to the accurate valuation of relevant lighter hydrocarbons oxidation bacterium primary in physical environment.And the culture condition that laboratory adopts also may make little microbe population originally be exaggerated, and occurs spurious anomaly.In addition, culture method complex operation, labour intensity is large, and incubation time long (needing 14～21 days), wastes time and energy, and can not meet the requirement of batch samples rapid detection.Therefore, how to improve Microbial Prospecting of Oil and Gas precision and efficiency is that domestic and international experts and scholars make great efforts the problem solving always.Real-Time Fluorescent Quantitative PCR Technique is a kind of new Protocols in Molecular Biology means, it does not need to cultivate, but directly from sample, extract bacteria total DNA, detect the functional gene content of indicator, detected result comprises can cultivate and not educable indicator, more accurate compared with culture method, quick (within 2～3 hours, can detect complete), high-throughput (can detect 96 samples for 1 time).
Real-time fluorescence quantitative PCR technology is as nucleic acid quantification method, in microbial ecology field, be widely used, as the dynamic monitoring changing for environment specified microflora, for the research of specified microflora physiological metabolism and the research of environmental microorganism community distribution.The application of this technology in Microbial Prospecting of Oil and Gas also has report, as adopt can culture technique and Real-Time Fluorescent Quantitative PCR Technique can detect respectively the methane-oxidizing bacteria of oil field aeration zone section different depths, result of study shows, adopts Real-Time Fluorescent Quantitative PCR Technique methane-oxidizing bacteria detected level than large 1～2 order of magnitude of methane-oxidizing bacteria detected level that can culture technique.
Microbial prospecting of oil and gas need to pay attention to indicating the selection of bacterial classification.Methane-oxidizing bacteria is a kind of obligate bacterium, can only utilize methane or methyl compounds as sole carbon source and the energy.Because methane content is more in the lighter hydrocarbons gas of hydrocarbon-bearing pool Upward Migration, can descend definitely the existence of hydrocarbon-bearing pool by measuring methane-oxidizing bacteria.But because the same degree of depth of non-HYDROCARBON-BEARING REGION also can detect the biogenesis methane of same amount, so the methane of the micro-migration of instruction bottom hydrocarbon-bearing pool that the methane-oxidizing bacteria near surface soil can not be single-minded, makes methane-oxidizing bacteria occur false-positive risk as reservoir exploration.Propane is also the important composition composition of micro-seepage hydrocarbon, and compared with the uncertainty in methane source, the unique source of propane is subterranean oil gas reservoir.Although its content in lighter hydrocarbons gas, not as methane, due to lasting Upward Migration, accumulates the oxidation of propane bacterium in soil cover in a large number, compared with soil background, quantity is extremely large, thereby can be by detecting this existence of extremely inferring hydrocarbon-bearing pool.Compared with methane-oxidizing bacteria, oxidation of propane bacterium is the more reliable indicator of hydrocarbon exploration.
Summary of the invention
For in current Microbial prospecting of oil and gas taking methane-oxidizing bacteria as main indicator, cannot distinguish hydrocarbon-bearing pool seepage methane or shallow-layer biogenesis methane, easily produce the drawback of " false positive " result, the invention provides a kind of based on fluorescent quantitative PCR technique, to detect oxidation of propane bacterial content extremely as main, methane oxidation bacterial content is auxiliary novel microorganism Petroleum Exploration Methods extremely.
For realizing the object of the invention, this method of extremely carrying out oil-gas exploration based on lighter hydrocarbons oxidation bacterium, is characterized in that comprising the following steps:
A. soil sample in oil-field development region or untapped region, soil sample dot density is 100 m～1000, m × 100 m × 1000 m, and sampling depth is 20 cm～250 cm, and sampling amount is 200 g～500 g;
B. the pedotheque above-mentioned sampling spot being obtained extracts sample DNA after processing through homogenizing, and oxidation of propane bacterium and methane-oxidizing bacteria quantity are wherein carried out to real-time fluorescence quantitative PCR mensuration; Wherein utilize propane monooxygenase prmA gene numerical value to represent oxidation of propane bacterium quantity, utilize methane monooxygenase pmoA numerical value to represent methane-oxidizing bacteria quantity;
C. determining of background value: adopt iterative method to reject after high value, determine iterations referring again to long profile method, in the time that the mean value of the new data set background value definite with growing profile method is close, stop iteration, the mean value X of new data set is worth as a setting, the mean value of definite new data set and standard deviation are thus: methane-oxidizing bacteria: X=33 copies/mg, SD=26.11; Oxidation of propane bacterium: X=27 copies/mg, SD=13.71;
D. the standard deviation S D that above-mentioned background value is added to 2 times of new data set is anomaly threshold C
0determine methane-oxidizing bacteria and oxidation of propane bacterium anomaly threshold, determine checking district methane-oxidizing bacteria and oxidation of propane bacterium maximum value, then will be greater than after the data Further Division exception level of anomaly threshold, set up the microbiological anomaly reference hierarchy of checking district Microbial Prospecting of Oil and Gas;
E. sample oxidation of propane bacterium detected result according to above-mentioned checking district's soil sample dot density, drawing isoline, and carry out microbiological anomaly subregion by above-mentioned reference hierarchy;
F. in microbiological anomaly subregion, according to methane oxidation bacterial content, will in abnormal subregion, be with gas-cap reservoir and pure oil reservoir to indicate.
Method used in the present invention is to extract to gather total DNA of pedotheque quantitative respectively to the oxidation of propane bacterium in pedotheque and methane-oxidizing bacteria by Real-Time Fluorescent Quantitative PCR Technique, oxidation of propane bacterial content between comparison different zones, determine oxidation of propane bacterium abnormal area, go out oxidation of propane bacterial content zone chart at exploration area chart display, the exceptions area that content is high is exploration target area, by oxidation of propane bacterium and methane-oxidizing bacteria content balance, further identification of hydrocarbon is hidden character subsequently.
The technical progress that the present invention obtains: oxidation of propane bacterium is extremely only present in hydrocarbon-bearing pool top near surface soil, if a certain region has higher oxidation of propane bacterial content, illustrates that the content of bottom hydrocarbon-bearing pool is higher.So it is comparatively accurate extremely to draw a circle to approve hydrocarbon-bearing pool scope with oxidation of propane bacterial content, can reduce the risk of the abnormal false positive results of methane oxidation bacterial content.
The present invention is taking hydrocarbon-bearing pool lighter hydrocarbons gas Vertical movement theory as basis, taking microorganism Protocols in Molecular Biology as means, design oxidation of propane bacterium is main indicator, taking methane-oxidizing bacteria as auxiliary indicator, reduce the false-positive impact of methane-oxidizing bacteria result, can more accurately carry out Microbial prospecting of oil and gas work.The lighter hydrocarbons oxidation bacterium of utilization of the present invention based on DNA analysis carries out oil-gas exploration extremely,, the defect such as incubation time long large with complex operation, the labour intensity of avoiding direct culture method to exist, aspect accuracy of detection, the directly quantity of lighter hydrocarbons oxidation bacterium in test sample, avoid the artificial interference in culture-based method, detected level is than high 1～2 order of magnitude of traditional cultural method, shortened to 12 hours by 14～21 days of traditional cultural method, and once can detect 96 samples, effectively improve precision and the detection efficiency of Microbial prospecting of oil and gas.
Brief description of the drawings
Fig. 1 is the abnormal subregion schematic diagram of oxidation of propane bacterium of the present invention.
Fig. 2 is the abnormal A level district reservoir properties prediction schematic diagram of Fig. 1.
" ╋ " shown in Fig. 1, Fig. 2 is sampling spot; "●" is producing well; " ▲ " is water injection well; " ■ " is band gas-cap reservoir; "
" be pure oil reservoir.
Embodiment
The present invention includes following steps:
A. soil sample in oil-field development region or untapped region, soil sample dot density is the grid sampling of 100 m～1000, m × 100 m × 1000 m, sampling depth is 20cm～250 cm, sampling amount is 200 g～500 g, the present embodiment adopts the grid sampling dot density of 250 m × 250 m, the present embodiment sampling depth 50 cm;
B., after the grinding bead homogenizer that the pedotheque above-mentioned sampling spot being obtained utilizes biospec company of the U.S. to produce homogenizes and processes, adopt the powerful soil DNA of mobio company of the U.S. to extract test kit and carry out DNA extraction.
C. the stepone quantitative real time PCR Instrument that utilizes American AB I company to produce carries out real-time fluorescence quantitative PCR mensuration to the propane monooxygenase prmA gene in sample, measures the quantity that numerical value can representative sample oxidation of propane bacterium.Employing primer is 955F(5 '-TGGCACCGGTGGATCTACGACGACT-3 ') and 1517R(5 '-GCGCGATCAGCGTCTTGCCGTC-3 '), build typical curve taking Uncultured soil bacterium clone SL01 (JX045657.1) as standard substance, the total reaction system of 20 μ l is: SYBR
premix Ex Taq
tM(2X) 10 μ l, front end primer (10 μ M) 0.2 μ l, rear end primer (10 μ M) 0.2 μ l, ROX Reference Dye (50 ×) 0.4 μ l, ultrapure water 7.2 μ l, DNA template 2 μ l, for oxidation of propane bacterium functional gene prmA, carry out fluorescent quantitative PCR condition optimizing, final definite by following amplification program: 94 DEG C of denaturation 30s, 94 DEG C of 5s, 66 DEG C of 30s, 72 DEG C of 60s, 88 DEG C of 15s(collect fluorescent signal) 40 circulations of amplification, 95 DEG C of 15s, 60 DEG C of 60s, 0.3 DEG C of reading 15s of 60 DEG C～95 DEG C every risings carries out melting curve analysis,
D. the stepone quantitative real time PCR Instrument that utilizes American AB I company to produce carries out real-time fluorescence quantitative PCR mensuration to the methane monooxygenase pmoA gene in sample, measures the quantity that numerical value can representative sample methane-oxidizing bacteria.Utilize pmoA gene realize to the quantitative process of methane-oxidizing bacteria in, the primer adopting is A189f (5 '-GGNGACTGGGACTTCTGG-3 ') and mb661R (5 '-CCGGMGCAACGTCYTTACC-3 '), taking Uncultured bacterium clone F05-FW300-98 particulate pmoA gene (DQ917359.1) as standard substance, the total reaction system of 20 μ l is: SYBR
premix Ex Taq
tM(2X) 10 μ l, front end primer (10 μ M) 0.2 μ l, rear end primer (10 μ M) 0.2 μ l, ROX Reference Dye (50 ×) 0.4 μ l, ultrapure water 7.2 μ l, DNA template 2 μ l, employing two step method is carried out, amplification program: first stage 95 DEG C of denaturation 30s; 95 DEG C of 5s of subordinate phase, 60 DEG C of 30s, 84 DEG C of 15s(collect fluorescent signal) 40 circulations of amplification; Phase III is drawn 95 DEG C of 15s of melting curve, 60 DEG C of 60s, 0.3 DEG C of reading of 60 DEG C～95 DEG C every risings, and reading duration 15s carries out melting curve analysis;
E. determining of background value: definite employing iterative method of background value is rejected after high value, determine iterations referring again to long profile method, in the time that the mean value of the new data set background value definite with growing profile method is close, stop iteration, the mean value X of new data set is worth as a setting, and the mean value of definite new data set and standard deviation are as follows thus:
Methane-oxidizing bacteria: X=33 copies/mg, SD=26.11
Oxidation of propane bacterium: X=27 copies/mg, SD=13.71
Accordingly, determine that checking district methane-oxidizing bacteria and oxidation of propane bacterium functional gene background value are respectively 33copies/mg and 27 copies/mg;
F. background value adds that the standard deviation S D of 2 times of new data set is anomaly threshold C
0accordingly, determine that methane-oxidizing bacteria and oxidation of propane bacterium anomaly threshold based on DNA analysis are respectively 90 copies/mg and 60 copies/mg, checking district methane-oxidizing bacteria and oxidation of propane bacterium maximum value are respectively 2673 copies/mg and 1589 copies/mg, the data Further Division exception level of anomaly threshold will be greater than, taking 200 copies/mg and 500 copies/mg as boundary, be defined as abnormal C level, abnormal B level and abnormal A level respectively.The microbiological anomaly reference hierarchy of setting up thus the Microbial Prospecting of Oil and Gas of checking district based on DNA analysis is as shown in table 1, and wherein abnormal A level district is favorable exploration target area.
The oil gas microbiological anomaly reference hierarchy of table 1 based on DNA analysis
In abnormal A level district, by oxidation of propane bacterial content and methane-oxidizing bacteria content balance, further identification of hydrocarbon is hidden character, and the measuring point that methane oxidation bacterial content is greater than 90 copies/mg underlies oil reservoir for band gas-cap reservoir, and the measuring point that is less than 90 copies/mg underlies, oil reservoir is pure oil reservoir, as shown in table 2.
The reservoir properties prediction of table 2 based on DNA analysis
G. according to above-mentioned checking district grid sampling oxidation of propane bacterium detected result, draw isopleth as shown in Figure 1, and carry out microbiological anomaly subregion by the reference hierarchy of setting up, result shows, verifies that western part, district is undeveloped region, east is oil-field development district, in the east oil district having exploited, producing well is distributed in exceptions area, near water injection well due to displacement of reservoir oil by filling water, bottom hydrocarbon-bearing pool is expelled, so neighbouring abnormal without oxidation of propane bacterium; Northern abnormal without oxidation of propane bacterium, represent that bottom is without hydrocarbon-bearing pool, producing well is not laid in oil field, thus oil producing region, east oxidation of propane bacterium abnormal distribute consistent with practical situation, and in the abnormal A level of the oxidation of propane bacterium district of western undeveloped region discovery for exploring Favorable Areas.
H. in abnormal A level district, according to methane oxidation bacterial content, the abnormal zone chart of oxidation of propane bacterium that abnormal A level is shown in as shown in Figure 2 with gas-cap reservoir and pure oil reservoir is in reservoir properties prediction schematic diagram.
Claims (1)
1. a method of extremely carrying out oil-gas exploration based on lighter hydrocarbons oxidation bacterium, is characterized in that comprising the following steps:
A. soil sample in oil-field development region or untapped region, soil sample dot density is 100 m～1000, m × 100 m × 1000 m, and sampling depth is 20 cm～250 cm, and sampling amount is 200 g～500 g;
B. the pedotheque above-mentioned sampling spot being obtained extracts sample DNA after processing through homogenizing, and oxidation of propane bacterium and methane-oxidizing bacteria quantity are wherein carried out to real-time fluorescence quantitative PCR mensuration; Wherein utilize propane monooxygenase prmA gene numerical value to represent oxidation of propane bacterium quantity, utilize methane monooxygenase pmoA numerical value to represent methane-oxidizing bacteria quantity;
C. determining of background value: adopt iterative method to reject after high value, determine iterations referring again to long profile method, in the time that the mean value of the new data set background value definite with growing profile method is close, stop iteration, the mean value X of new data set is worth as a setting, the mean value of definite new data set and standard deviation are thus: methane-oxidizing bacteria: X=33 copies/mg, SD=26.11; Oxidation of propane bacterium: X=27 copies/mg, SD=13.71;
D. the standard deviation S D that above-mentioned background value is added to 2 times of new data set is anomaly threshold C
0determine methane-oxidizing bacteria and oxidation of propane bacterium anomaly threshold, determine checking district methane-oxidizing bacteria and oxidation of propane bacterium maximum value, then will be greater than after the data Further Division exception level of anomaly threshold, set up the microbiological anomaly reference hierarchy of checking district Microbial Prospecting of Oil and Gas;
E. sample oxidation of propane bacterium detected result according to above-mentioned checking district's soil sample dot density, drawing isoline, and carry out microbiological anomaly subregion by above-mentioned reference hierarchy;
F. in microbiological anomaly subregion, according to methane oxidation bacterial content, will in abnormal subregion, be with gas-cap reservoir and pure oil reservoir to indicate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410249530.XA CN103981277A (en) | 2014-06-06 | 2014-06-06 | Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410249530.XA CN103981277A (en) | 2014-06-06 | 2014-06-06 | Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103981277A true CN103981277A (en) | 2014-08-13 |
Family
ID=51273448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410249530.XA Pending CN103981277A (en) | 2014-06-06 | 2014-06-06 | Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103981277A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106126975A (en) * | 2016-06-28 | 2016-11-16 | 中国地质科学院水文地质环境地质研究所 | Gene quantification Microbial prospecting of oil and gas method based on mathematical statistics |
| CN106480167A (en) * | 2015-08-24 | 2017-03-08 | 中国石油化工股份有限公司 | A kind of method of utilization microbial prospecting oil gas |
| CN107532198A (en) * | 2015-02-12 | 2018-01-02 | 生物识别有限公司 | The detection and production of the computer supported to the inhomogeneous distribution formula hydrocarbon source in subsurface formations based on microbial prospecting |
| CN108265108A (en) * | 2016-12-30 | 2018-07-10 | 中国石油化工股份有限公司 | A kind of method of methane-oxidizing bacteria in monitoring soil |
| CN109423458A (en) * | 2017-08-30 | 2019-03-05 | 中国石油化工股份有限公司 | A kind of Rhodococcus sp and its identification method and application |
| CN111458480A (en) * | 2020-04-10 | 2020-07-28 | 盎亿泰地质微生物技术（北京）有限公司 | Method and device for detecting coal field combustion area |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234608A (en) * | 2010-05-06 | 2011-11-09 | 长江大学 | Production method of propane oxidizing bacteria test bottle |
-
2014
- 2014-06-06 CN CN201410249530.XA patent/CN103981277A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234608A (en) * | 2010-05-06 | 2011-11-09 | 长江大学 | Production method of propane oxidizing bacteria test bottle |
Non-Patent Citations (1)
| Title |
|---|
| 邵明瑞: "油气田土壤丙烷氧化菌的检测及油气藏勘探应用", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107532198A (en) * | 2015-02-12 | 2018-01-02 | 生物识别有限公司 | The detection and production of the computer supported to the inhomogeneous distribution formula hydrocarbon source in subsurface formations based on microbial prospecting |
| CN106480167A (en) * | 2015-08-24 | 2017-03-08 | 中国石油化工股份有限公司 | A kind of method of utilization microbial prospecting oil gas |
| CN106480167B (en) * | 2015-08-24 | 2020-01-24 | 中国石油化工股份有限公司 | Method for exploring oil gas by using microorganisms |
| CN106126975A (en) * | 2016-06-28 | 2016-11-16 | 中国地质科学院水文地质环境地质研究所 | Gene quantification Microbial prospecting of oil and gas method based on mathematical statistics |
| CN106126975B (en) * | 2016-06-28 | 2020-06-26 | 中国地质科学院水文地质环境地质研究所 | Gene quantitative microbial oil and gas exploration method based on mathematical statistics |
| CN108265108A (en) * | 2016-12-30 | 2018-07-10 | 中国石油化工股份有限公司 | A kind of method of methane-oxidizing bacteria in monitoring soil |
| CN109423458A (en) * | 2017-08-30 | 2019-03-05 | 中国石油化工股份有限公司 | A kind of Rhodococcus sp and its identification method and application |
| CN109423458B (en) * | 2017-08-30 | 2022-08-19 | 中国石油化工股份有限公司 | Rhodococcus and identification method and application thereof |
| CN111458480A (en) * | 2020-04-10 | 2020-07-28 | 盎亿泰地质微生物技术（北京）有限公司 | Method and device for detecting coal field combustion area |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103981277A (en) | Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria | |
| Xiong et al. | Geographic distance and pH drive bacterial distribution in alkaline lake sediments across Tibetan Plateau | |
| Martiny et al. | Taxonomic resolution, ecotypes and the biogeography of Prochlorococcus | |
| US10663618B2 (en) | Methods to determine conditions of a hydrocarbon reservoir | |
| Hodgkinson et al. | Background research for selection of potential geostorage targets—case studies from the Surat Basin, Queensland | |
| Sorensen et al. | Using boreholes as windows into groundwater ecosystems | |
| Kühn et al. | CLEAN: Project overview on CO 2 large-scale enhanced gas recovery in the Altmark natural gas field (Germany) | |
| CN103776981B (en) | A kind of new karst stage time method of discrimination | |
| CN104101914A (en) | Method for oil-gas resource exploration indicating by utilizing molecular ecology | |
| CN106769170B (en) | A kind of acquisition method of karst underground hole, crack and soil information | |
| CN112301138A (en) | Oil gas microbial gene exploration method | |
| Du et al. | Landscape position influences microbial composition and function via redistribution of soil water across a watershed | |
| Hou et al. | Quantitative assessment of the sweet spot in marine shale oil and gas based on geology, engineering, and economics: A case study from the Eagle Ford Shale, USA | |
| Flynn et al. | The active bacterial community in a pristine confined aquifer | |
| US10619458B2 (en) | Computer supported exploration and production of heterogeneous distributed hydrocarbon sources in subsurface formations based on microbial prospecting | |
| CN106126975B (en) | Gene quantitative microbial oil and gas exploration method based on mathematical statistics | |
| CN102676643A (en) | Effective microbiological detection method capable of being applied to oil-gas exploration | |
| CN107267623B (en) | A kind of Microbial Prospecting of Oil and Gas method | |
| Yonkofski et al. | Risk-based monitoring designs for detecting CO2 leakage through abandoned wellbores: An application of NRAP’s WLAT and DREAM tools | |
| Sawadogo et al. | Impact of Completion Design and Interwell Communication on Well Performance in Full Section Development: A STACK Case Study Using DNA Based Diagnostics | |
| CN106480167A (en) | A kind of method of utilization microbial prospecting oil gas | |
| He et al. | Genetic quantitative techniques combined with continuous electromagnetic profiling to identify subtle oil and gas reservoirs | |
| CN113702620A (en) | Method for determining hydrocarbon source by using microbial fingerprint | |
| CN114958964B (en) | Oil gas exploration method based on microbial genes | |
| Posdyshev et al. | Using DNA-Logging to Determine Inflow Profile in Horizontal Wells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140813 |