CN103981240A - Method of increasing the saccharification reaction efficiency of high-concentration starch - Google Patents
Method of increasing the saccharification reaction efficiency of high-concentration starch Download PDFInfo
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- CN103981240A CN103981240A CN201410201603.8A CN201410201603A CN103981240A CN 103981240 A CN103981240 A CN 103981240A CN 201410201603 A CN201410201603 A CN 201410201603A CN 103981240 A CN103981240 A CN 103981240A
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Abstract
The invention discloses a method of increasing the saccharification reaction efficiency of high-concentration starch, and belongs to the technical field of starch saccharification. According to the method, a high-concentration starch liquefaction product is adopted as a substrate, glucoamylase is added, an ultrasonic pretreatment method is adopted to increase the saccharification reaction efficiency, and finally the production cost of glucose is reduced. The concentration of the substrate is increased from 30% in industry to about 50%, thus reducing the evaporation energy consumption of glucose solutions in the later period of starch saccharification, and overcoming the disadvantages such as high water consumption and energy consumption of the starch saccharification.
Description
Technical field
The present invention relates to a kind of method that improves high density mashing reaction efficiency, belong to starch refine dsugar technical field.
Background technology
Along with the raising of people's living standard, the demand of β-amylose is increasing.In β-amylose tradition enzymatic production process, starch milk starting point concentration is controlled at 25%～35% conventionally, relatively low concentration can be brought the problems such as high water consumption, high energy consumption, in addition, in some amino acid, antibiotic fermentation, need to add more than 40% liquid glucose as carbon source, and the liquid glucose that traditional technology obtains need to concentrate and could use, can increase the cost of these leavened prods, therefore, put forth effort on raising starch milk starting point concentration very necessary.But along with the raising of concentration of substrate, can there is obvious decline in mashing reaction efficiency, is unfavorable for the control of β-amylose production cost.
At present, both at home and abroad to high density starch liquefacation, the existing certain research of Mashing process, and the research of relevant ultrasonic pretreatment raising high density mashing efficiency has no report.Therefore to select starch liquefacation product be substrate in the present invention, through ultrasonic pretreatment, to improve saccharification react speed and the glucose yield of the catalysis of glucoamylase institute.
Summary of the invention
The present invention is mainly to provide a kind of method that improves high density mashing reaction efficiency, is taking high density starch liquefacation product as substrate, through ultrasonic pretreatment, to improve saccharification react speed and the glucose yield of the catalysis of glucoamylase institute.
Described method is taking starch liquefacation product as substrate, adopts ultrasonic pretreatment to improve reaction system mobility, then prepares glucose taking glucoamylase as saccharifying enzyme.
Described high density starch starting point concentration is 40%-60% (w/w, in butt), and after high temperature resistant α-amylaseliquefied, the DE value of starch liquefacation product is 5-25.
Described ultrasonication condition optimization: taking starch liquefacation product as substrate, with hydrochloric acid adjust pH to 4.0-5.0, add glucoamylase 20-100U/g starch (in butt), ultrasonic power is 100-200W, the ultrasonic pretreatment time is 30-180s, saccharification react temperature 40-60 DEG C, reaction times 12-72h.The preferred 0.05-0.1kg of reaction system of described ultrasonic pretreatment.
Described ultrasonication condition is further preferred: taking starch liquefacation product as substrate, with hydrochloric acid adjust pH to 4.5, add glucoamylase 50U/g starch (in butt), carry out ultrasonication: ultrasonic power is 125W, the ultrasonic pretreatment time is 90s, wherein the working hour is 3s, and the intermittent time is 3s.
Beneficial effect of the present invention: than without ultrasonication, can obviously accelerate the saccharification speed of glucoamylase to starch liquefacation product by ultrasonic pretreatment, meanwhile improve glucose yield.After ultrasonication, hyperacoustic energy part has passed to substrate molecule and enzyme molecule, has accelerated molecular thermalmotion, has improved mutual collision probability, thereby has improved saccharification efficiency; The cavatition that ultrasonic wave produces can change the formal structure of substrate on the one hand, and on the other hand, the energy of the shockwave of its generation is enough in saboteur and intermolecular chemical bond, thereby has the effect that reduces system viscosity.Concentration of substrate of the present invention is brought up to 50% left and right by 30% industrial left and right, can reduce starch refine dsugar later stage evaporation energy consumption, thereby overcomes the defects such as starch refine dsugar water consumption is high, energy consumption is high.
Brief description of the drawings
The just relation of speed of Fig. 1 ultrasonic time and reaction.
The impact of Fig. 2 ultrasonic pretreatment time on glucose yield.
Embodiment
Detect final saccharification product by high performance liquid chromatography (HPLC).
Embodiment 1 ultrasonic pretreatment is on the just impact of speed of high density mashing reaction
Technological process:
Starting point concentration is 50% (w/w, in butt) starch milk after high temperature resistant α-amylaseliquefied, the DE value of controlling starch liquefacation product is 15 left and right, add 50U/g glucoamylase (Genencor Company's product), Ultrasonic Conditions is: reaction solution pH is 4.5, and ultrasonic power is 125W, and pretreatment time is 0-180s, wherein the working hour is 3s, and the intermittent time is 3s.Saccharification react temperature 60 C, reaction times 10min.Measure the just relation of speed of ultrasonic time and reaction.Result as shown in Figure 1.
Show from reaction result, the ultrasonic pretreatment of certain hour can be accelerated the saccharification speed of glucoamylase to starch significantly.Not ultrasonic saccharified liquid reaction just speed is 1.8g/ (Lmin), and the reaction of ultrasonic 90s (power 125W) just speed ratio blank has improved 31.5%.Continue to extend ultrasonic time, can reduce on the contrary speed of reaction.
The impact of embodiment 2 ultrasonic pretreatment on high density mashing reaction process
Technological process:
Starting point concentration is 50% (w/w, in butt) starch milk after high temperature resistant α-amylaseliquefied, the DE value of controlling starch liquefacation product is 15 left and right, add 50U/g glucoamylase, Ultrasonic Conditions is: reaction solution pH is 4.5, and ultrasonic power is 125W, pretreatment time is 90s, wherein the working hour is 3s, and the intermittent time is 3s, and reaction system is 0.05kg.Saccharification react temperature 60 C, reaction times 48h.Measure the impact of ultrasonic pretreatment on saccharifying, process ultrasonic pretreatment is not blank group.Result as shown in Figure 2.
Experimental result demonstration, after ultrasonic pretreatment, mashing speed of reaction in early stage obviously improves.After saccharification 24h, the glucose yield of the sample of ultrasonic pretreatment is glucose yield when 48h higher than contrast, illustrates that ultrasonic pretreatment can shorten saccharification time.And continue to extend the saccharification 24h time, glucose yield increasess slowly.
Determining of embodiment 3 ultrasonic pretreatment reaction conditionss
(1) best power of ultrasonic pretreatment: varying in size of ultrasonic power, just different for the facilitation effect of saccharification react.Different ultrasonication power is as shown in table 1 with the relation of final glucose yield.
Experimental result demonstration, the ultrasonication of appropriateness can improve glucose yield.In the time that ultrasonic power is too high, glucose yield can decline, and this may be relevant with high strength supersonic meeting affecting glucose amylase activity.Therefore, select power 125W ultrasonic pretreatment reaction solution the best.
Table 1
(2) impact of ultrasonic time on glucose yield under certain power ultrasonic pre-treatment.
Under the supersound process of power 125W, the relation of different ultrasonic times and glucose yield is as shown in table 2.
Experimental result shows, within the scope of 0-90s, along with the increase of ultrasonic time, glucose yield is corresponding increase also, taking time of ultrasonic 90s as best.
Table 2
Although the present invention with preferred embodiment openly as above; but it is not in order to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, therefore protection scope of the present invention should be with being as the criterion that claims were defined.
Claims (6)
1. a method that improves high density mashing reaction efficiency, is characterized in that, is taking high density starch liquefacation product as substrate, through ultrasonic pretreatment, to improve saccharification react speed and the glucose yield of the catalysis of glucoamylase institute.
2. method according to claim 1, is characterized in that, described high density starch liquefacation product is initial mass concentration to be counted to the starch of 40%-60% with butt through high temperature resistant α-amylaseliquefied processing, and the DE value of starch liquefacation product is 5-25.
3. method according to claim 1, it is characterized in that, described ultrasonic pretreatment condition is: taking starch liquefacation product as substrate, with hydrochloric acid adjust pH to 4.0-5.0, add glucoamylase in butt: 20-100U/g starch, ultrasonic power is 100-200W, the ultrasonic pretreatment time is 30-180s, wherein the working hour is 3s, and the intermittent time is 3s.
4. method according to claim 3, is characterized in that, the consumption of described glucoamylase is preferably in butt 50U/g starch.
5. method according to claim 3, is characterized in that, described ultrasonic pretreatment condition optimization: power is 125W, and the ultrasonic pretreatment time is 90s, and wherein the working hour is 3s, and the intermittent time is 3s.
6. according to the arbitrary described method of claim 1-5, it is characterized in that saccharification react temperature 40-60 DEG C, reaction times 12-72h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263781A (en) * | 2014-09-29 | 2015-01-07 | 江苏大学 | Ultrasonic-assisted double-enzyme hydrolysis technology of starch |
| CN108949864A (en) * | 2018-08-07 | 2018-12-07 | 梁山菱花生物科技有限公司 | The preparation method and application of glutamic acid fermentation sugar |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787382A (en) * | 2010-01-19 | 2010-07-28 | 广州双桥股份有限公司 | Method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787382A (en) * | 2010-01-19 | 2010-07-28 | 广州双桥股份有限公司 | Method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup |
Non-Patent Citations (1)
| Title |
|---|
| STEPHEN BARTON ET AL.: "The effects of ultrasound on the activities of some glycosidase enzymes of industrial importance", 《ENZYME AND MICROBIAL TECHNOLOGY》, vol. 18, 15 February 1996 (1996-02-15), pages 190 - 194, XP 002071669, DOI: doi:10.1016/0141-0229(95)00092-5 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263781A (en) * | 2014-09-29 | 2015-01-07 | 江苏大学 | Ultrasonic-assisted double-enzyme hydrolysis technology of starch |
| CN108949864A (en) * | 2018-08-07 | 2018-12-07 | 梁山菱花生物科技有限公司 | The preparation method and application of glutamic acid fermentation sugar |
| WO2020029353A1 (en) * | 2018-08-07 | 2020-02-13 | 梁山菱花生物科技有限公司 | Sugar for glutamic acid fermentation, and preparation method therefor and application thereof |
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