CN103981132A - Arthrobacterium and application thereof in degradation of aflatoxin - Google Patents

Arthrobacterium and application thereof in degradation of aflatoxin Download PDF

Info

Publication number
CN103981132A
CN103981132A CN201410203045.9A CN201410203045A CN103981132A CN 103981132 A CN103981132 A CN 103981132A CN 201410203045 A CN201410203045 A CN 201410203045A CN 103981132 A CN103981132 A CN 103981132A
Authority
CN
China
Prior art keywords
aflatoxin
afb
degradation
arthrobacter
afg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410203045.9A
Other languages
Chinese (zh)
Other versions
CN103981132B (en
Inventor
刘阳
赵月菊
兰茨纳·桑格
娅娃·米妮·埃尔迪·富丽
邢福国
周露
王龑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Food Science and Technology of CAAS
Original Assignee
Institute of Food Science and Technology of CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Food Science and Technology of CAAS filed Critical Institute of Food Science and Technology of CAAS
Priority to CN201410203045.9A priority Critical patent/CN103981132B/en
Publication of CN103981132A publication Critical patent/CN103981132A/en
Application granted granted Critical
Publication of CN103981132B publication Critical patent/CN103981132B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses an arthrobacterium and an application thereof in degradation of aflatoxin and provides an arthrobacterium (Arthrobacter sp.) L15 which is assigned the accession number CGMCC No.8869. An experiment in the invention proves that the arthrobacterium L15 is discovered in the invention. Aflatoxin can be degraded efficiently when a fermentation liquor of the arthrobacterium L15 is added to the aflatoxin and dissolving and uniform mixing are carried out. The arthrobacterium L15, used as a biological material for degrading aflatoxin, has good application prospects in exploitation of not only novel biodegradation inoculants but also novel biodegradation sterile preparations.

Description

One strain Arthrobacter and the application in aflatoxin degradation thereof
Technical field
The present invention relates to biological technical field, relate in particular to a strain Arthrobacter and the application in aflatoxin degradation thereof.
Background technology
Aflatoxin (Aflatoxin, AFT) be that a class is mainly by flavus (Aspergillus flavus) and the mycetogenetic poisonous secondary metabolite of Aspergillus parasiticus (A.parasiticus), there is carcinogenic, teratogenesis, cause the effect of cell mutation, only 0.294mg/kg dosage just can cause acute poisoning dead (Bondy and Pestka, 2000 of Sensitivity animal; Wang et al., 2002; Rawal, et al., 2010), its main action target is liver, to bring out malignant tumour primary hepatocellular carcinoma (Hepatocellular carcinoma, HCC) one of principal element, can cause kidney and adrenal acute pathology (Poirier et al., 2000 in addition; Kensler et al., 2011).
The basic structure of aflatoxin is two furan nucleuss and coumarin (tonka bean camphor), and the former is basic toxicity structure, and the latter has the toxicity of reinforcement and carcinogenesis.The aflatoxin of having found at present approximately has 20 kinds, and what in UV-light, issue blue light (Blue) is B family (AFB 1and B 2), green light (Green) be G family (AFG 1and G 2).Aflatoxin M 1aFB 1hydroxylation derivative.AFB wherein 1distribute the widest, content is the highest, toxicity is the strongest, its toxicity is 10 times of potassium cyanide, 68 times of arsenic.
Traditional aflatoxin detoxicating method has physics and chemistry method, comprise ammoniation process, alkaline process, pyroprocess, x ray irradiation x method and ultrafiltration-percolation process etc., these methods exist that effect is unstable, nutritive ingredient loss is large, degraded product is complicated, degraded product toxicity is difficult to determine, and is difficult to the shortcomings such as large-scale production; In addition, also have absorption method, absorption method also can be adsorbed nutritive ingredient in absorbing toxin.Biological process is the method for utilizing the meta-bolites aflatoxin degradations such as enzyme of microorganism and secretion thereof.The feature that biological process has that efficiency is high, high specificity and food, feed and environment are not had pollutes is the direction of aflatoxin degraded.
Summary of the invention
An object of the present invention is to provide a strain Arthrobacter.
A strain Arthrobacter provided by the invention (Arthrobacter sp.) L15, its deposit number is CGMCC No.8869.
Above-mentioned Arthrobacter L15 and/or its bacteria suspension and/or its fermented liquid and/or the application of its meta-bolites in aflatoxin degradation are also the scope of protection of the invention.
Above-mentioned Arthrobacter L15 and/or its bacteria suspension and/or its fermented liquid and/or the application of its meta-bolites in preparing the product of aflatoxin degradation are also the scope of protection of the invention.
In above-mentioned application, described aflatoxin is AFB 1, AFB 2, AFG 1, AFG 2or aflatoxin M 1.
Another object of the present invention is to provide a kind of method of aflatoxin degradation.
Method provided by the invention, comprises the steps:, with above-mentioned Arthrobacter L15, aflatoxin is carried out to degradation treatment.
In aforesaid method, the mode of described degradation treatment is by the bacteria suspension of described Arthrobacter L15 and/or its fermented liquid and/or its meta-bolites and the sample and/or the Product mix that contain aflatoxin.
The described sample that contains aflatoxin and/or product are specially processing of farm products raw material, feed, food and environmental sample and/or the product that contains aflatoxin.
In aforesaid method, described aflatoxin is derivative and/or the G family aflatoxin of B family aflatoxin, B family aflatoxin, and wherein, described B family aflatoxin is AFB 1or AFB 2; Described G family aflatoxin is AFG 1or AFG 2.The derivative of described B family aflatoxin is aflatoxin M 1.In the present invention, described aflatoxin is AFB 1, AFB 2, AFG 1, AFG 2or aflatoxin M 1.
The described sample that contains aflatoxin and/or product are specially processing of farm products raw material, feed, food and environmental sample and/or the product that contains aflatoxin.
In an embodiment of the present invention, described degradation treatment is that the nutrient solution of Arthrobacter L15 mixes with aflatoxin solution; Aflatoxin solution is comprised of aflatoxin and Chromatographic Pure Methanol, and the final concentration of aflatoxin in aflatoxin solution is 100ppb.
Arthrobacter L15 nutrient solution is for to be inoculated in Arthrobacter (Arthrobacter sp.) L15 (CGMCC No.8869) in liquid NB substratum, in temperature, be that 28 ℃ and rotating speed are under the condition of 200rpm (rotation radius 20mm) after shaking culture 24h, all substances in culture vessel are denoted as L15 nutrient solution.
Bacterial strain L15 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on February 28th, 2014 and (is called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), preserving number is CGMCC No.8869, and Classification And Nomenclature is Arthrobacter Arthrobacter sp..
Of the present invention experimental results show that, the present invention has found a strain Arthrobacter L15, its fermented liquid and aflatoxin dissolving are mixed, it can efficient degradation aflatoxin, therefore this bacterium, as the biomaterial of aflatoxin degraded, all has good application prospect aspect the new biological degradation microbial inoculum of exploitation or biological degradation sterile preparation.
Accompanying drawing explanation
Fig. 1 is AFB 1degradation effect liquid chromatogram
Fig. 2 is AFB 2degradation effect liquid chromatogram
Fig. 3 is AFG 1degradation effect liquid chromatogram
Fig. 4 is AFG 2degradation effect liquid chromatogram
Fig. 5 is aflatoxin M 1degradation effect liquid chromatogram
Embodiment
The experimental technique using in following embodiment if no special instructions, is ordinary method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
NB liquid nutrient medium: formed by solvent and solute; Solute is peptone, extractum carnis and NaCl, and solvent is water; The concentration of peptone in NB liquid nutrient medium is 1%, and the concentration of extractum carnis in NB liquid nutrient medium is that the concentration of 0.3%, NaCl in NB liquid nutrient medium is 0.5%, and described % is quality volume percent (g/100ml).
NA solid medium: add agar (ratio of agar and liquid nutrient medium is 1.5g:100ml) in NB liquid nutrient medium, obtain NA solid medium.
The separation of embodiment 1, bacterium and evaluation
One, the separation of bacterium
In June, (one) 2013, in Bechtop, is placed on collection in sterile distilled water and shakes and within 15 minutes, prepare bacteria suspension from Beijing suburb pedotheque, and shaking speed is 180rpm.
(2) bacteria suspension is carried out being coated on NA culture medium flat plate after concentration gradient dilution with sterile distilled water, under 30 ℃ of conditions, cultivate 24 hours, bacterium colony is covered with whole flat board, with form on transfering loop picking flat board, size, color, bacterial strain plate streaking that transparency is different, purify, the bacterial strain that point connects after purifying is applied to aflatoxin degradation experiment.The strong bacterial strain called after L15 of a strain aflatoxin degradation capability obtaining.
Two, identify
1, physiology and morphology biochemical characteristic
According to " the outstanding Bacteria Identification handbook of uncle " (the 8th edition) and " common bacteria system identification handbook " (eastern elegant pearl, Cai Miaoying etc. write, Beijing: Science Press, 2001.2) method of describing in, bacterial strain L15 is carried out to morphological specificity and physio-biochemical characteristics evaluation, and concrete outcome is as follows:
The physiology and morphology biochemical characteristic of thalline:
Be shaft-like; Gram-positive; Oxydase :-; Catalase :+; Starch Hydrolysis :+; Casein hydrolysis :-; Nitrate reduction :+; 50 ℃ of growths :-.
Biolog GEN III growth experiment shows, bacterial strain L15 can utilize a-D-glucose, p-hydroxyl phenylacetic acid, dextrin, D-MANNOSE, Pyruvic Acid Methyl ester, D-Maltose, D-Fructose, ALANINE, D-ALPHA-Hydroxypropionic acid methyl esters, D-trehalose, D-semi-lactosi, L-arginine, Pfansteihl, D-cellobiose, L-Aspartic acid, citric acid, gentiobiose, D-Fucose, Pidolidone, α-ketoglutaric acid, sucrose, L-fucose, L-Histidine, turanose, L-rhamnosyl, L-Glutimic acid, L MALIC ACID, stachyose, inosine, Serine, bromosuccinic acid, D-raffinose, pectin, PEARLITOL 25C, γ-aminobutyric acid, D-melibiose, α-hydroxybutyric acid, Beta-methyl-D-glucoside, D-glyconic acid, beta-hydroxy-D, L-butyric acid, D-saligenin, glycerine, D-Glucose aldehydic acid, α-batanone acid, N-acetyl-GLUCOSAMINE, etheric acid, propionic acid, quininic acid and acetic acid.
Biolog GEN III chemical-sensitive is tested and is shown, bacterial strain L15 is responsive to condition determinations such as 1% Sodium.alpha.-hydroxypropionate, Nalidixic Acid, pH6.0, lithium chloride, pH5.0, aztreonam, 1%NaCl, Sodium propanecarboxylate, 4%NaCl and sodium bromates.
Insensitive to institute's condition determinations such as lincomycin, fusidic acid, Guanidinium hydrochloride, D-Ser, sodium tetradecyl sulfate, troleomycin, vancomycin, Rifamycin Sodium, tetrazolium violet, 8%NaCl, MINOCYCLINE HCL, ditetrazolium chlorides.
2,16S rDNA test
Extract total DNA of L15, take it as template, utilize bacterial 16 S rDNA universal primer (27f:AGAGTTTGATCCTGGCTCAG; 1492r:GGTTACCTTGTTACGACTT) carry out pcr amplification, obtain the amplified production that length is about 1.3kb, amplified production is reclaimed and check order, the nucleotides sequence of amplified production is classified the sequence 1 in sequence table as.
According to the comparison of Gen-Bank sequence homology, bacterial strain L15 and Arthrobacter sp.bE58 (2011) (GenBank accession number JF772506.1) homology is 99%, and this bacterium of preliminary judgement is arthrobacter bacteria (Arthrobacter sp.).
Based on above feature, bacterial strain L15 is accredited as to Arthrobacter (Arthrobacter sp.).This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on February 28th, 2014 and (is called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), preserving number is CGMCC No.8869, and Classification And Nomenclature is Arthrobacter Arthrobacter sp..
Embodiment 2, Arthrobacter L15 are to aflatoxin Degradation
One, cultivate activation
Arthrobacter (Arthrobacter sp.) L15CGMCC No.8869 is inoculated in liquid NB substratum, in temperature, be that 28 ℃ and rotating speed are under the condition of 200rpm (rotation radius 20mm) after shaking culture 24h, all substances in culture vessel are denoted as L15 nutrient solution L15 nutrient solution, for follow-up aflatoxin degradation experiment.
Two, Arthrobacter L15 is to AFB 1degradation
1, AFB 1solution (AFB 1) preparation:
By 1mg AFB 1standard substance ( article No. A6636) be dissolved in and in 2ml Chromatographic Pure Methanol, obtain the AFB that concentration is 500ppb 1solution.
2, degradation experiment
Experimental group: the L15 nutrient solution of getting the above-mentioned acquisition of 0.4ml is placed in 1.5ml centrifuge tube, and adding 0.1ml concentration is the AFB of 500ppb 1solution to its final concentration is 100ppb, fully mixes after 37 ℃ of standing 72h, and the centrifugal 10min of 10000g removes cell.
Control group: the NB substratum that the 0.4ml of take does not connect bacterium adds the AFB that 0.1ml concentration is 500ppb 1.
3, AFB 1detection:
First use methyl alcohol: water (6:4) solution is to experimental group supernatant liquor, control group and AFB 1standard substance extract respectively, then use immune affine decontaminating column to purify extraction (method is with reference to immune affinity column working instructions) to the residual toxin of sample; Finally with HPLC-TOF-MS, purification being extracted to the sample obtaining detects.
HPLC testing conditions is mobile phase methanol: water=1:1; Flow velocity 1ml/min; Chromatographic column C18150mm * 4.6mm, 0.5 μ m; Excitation wavelength 350nm, detects wavelength 450nm; 30 ℃ of column temperatures; Sample size 20 μ l.
AFB 1degradation rate (%)=(residual AFB of control group 1the residual AFB of content-experimental group 1content) the residual AFB of/control group 1content * 100.
Result as shown in Fig. 1 and table 1, A: aflatoxin standard substance (AFB 1retention time be 13.009min); B: control group (AFB 1retention time be 13.660min); C: experimental group (AFB 1retention time be 13.126min);
Residual AFB in control group 1content is 98.65 ± 3.86ppb;
Residual AFB in experimental group 1content is 21.34 ± 2.46ppb;
Result shows, L15 is to AFB 1there is good degradation effect, degradation rate 78.37 ± 2.49%.
Three, Arthrobacter L15 is to AFB 2degradation
1, AFB 2preparation:
By 1mg AFB 2standard substance ( article No. A9887) be dissolved in and in 2ml Chromatographic Pure Methanol, obtain the AFB that concentration is 500ppb 2solution.
2, degradation experiment
Experimental group: basic identical with above-mentioned two method, different is to replace with AFB 2solution.
Control group: identical with above-mentioned two method.
3, AFB 2detection:
Identical with above-mentioned two method.
AFB 2degradation rate (%)=(residual AFB of control group 2the residual AFB of content-experimental group 2content) the residual AFB of/control group 2content * 100.
Result as shown in Figure 2 and Table 1, A: aflatoxin standard substance (AFB 2retention time be 11.401min); B: control group (AFB 1retention time be 11.382min); C: experimental group (AFB 1retention time be 11.310min);
Residual AFB in control group 2content is 105.89 ± 1.69ppb;
Residual AFB in experimental group 2content is 53.58 ± 2.89ppb.
Result shows, L15 is to AFB 2have certain degradation effect, degradation rate is 49.40 ± 2.73%.
Four, Arthrobacter L15 is to AFG 1degradation
1, AFG 1preparation:
By 1mg AFG 1standard substance ( article No. 32756) be dissolved in and in 2ml Chromatographic Pure Methanol, obtain the AFG that concentration is 500ppb 1solution.
2, degradation experiment
Experimental group: basic identical with above-mentioned two method, different is to replace with AFG 1solution.
Control group: identical with above-mentioned two method.
3, AFG 1detection:
Identical with above-mentioned two method.
AFG 1degradation rate (%)=(residual AFG of control group 1the residual AFG of content-experimental group 1content) the residual AFG of/control group 1content * 100.
Result as shown in Figure 3 and Table 1, A: aflatoxin standard substance (AFG 1retention time be 9.706min); B: control group (AFG 1retention time be 9.746min); C: experimental group;
Residual AFG in control group 1content is 101.19 ± 3.17;
Residual AFG in experimental group 1content is 0ppb.
Result shows, L15 is to AFG 1have good degradation effect, degradation rate is 100 ± 0%.
Five, Arthrobacter L15 is to AFG 2degradation
1, AFG 2preparation:
By 1mg AFG 2standard substance ( article No. A0263) be dissolved in and in 2ml Chromatographic Pure Methanol, obtain the AFG that concentration is 500ppb 2solution.
2, degradation experiment
Experimental group: basic identical with above-mentioned two method, different is to replace with AFG 2solution.
Control group: identical with above-mentioned two method.
3, AFG 2detection:
Identical with above-mentioned two method.
AFG 2degradation rate (%)=(residual AFG of control group 2the residual AFG of content-treatment group 2content) the residual AFG of/control group 2content * 100.
Result as shown in Figure 4 and Table 1, A: aflatoxin standard substance (AFG 2retention time be 8.681min); B: control group (AFG 1retention time be 8.902min); C: experimental group;
Residual AFG in control group 2content is 83.42 ± 2.47bbp;
Residual AFG in experimental group 2content is 0bbp.
Result shows, L15 is to AFG 2have good degradation effect, degradation rate is 100 ± 0%.
Six, Arthrobacter L15 is to aflatoxin M 1degradation
1, aflatoxin M 1preparation:
By 1mg aflatoxin M 1standard substance ( article No. 34031) be dissolved in and in 2ml Chromatographic Pure Methanol, obtain the aflatoxin M that concentration is 500ppb 1solution.
2, degradation experiment
Experimental group: basic identical with above-mentioned two method, different is to replace with aflatoxin M 1solution.
Control group: identical with above-mentioned two method.
3, aflatoxin M 1detection:
Identical with above-mentioned two method.
AFM 1degradation rate (%)=(residual AFM of control group 1the residual AFM of content-experimental group 1content) the residual AFM of/control group 1content * 100.
Result as shown in Fig. 5 and table 1, A: aflatoxin standard substance (aflatoxin M 1retention time be 7.847min); B: control group (aflatoxin M 1retention time be 7.866min); C: experimental group (aflatoxin M 1retention time be 7.920min);
Residual aflatoxin M in control group 1content is 92.97 ± 0.56bbp;
Residual aflatoxin M in experimental group 1content is 16.43 ± 0.41bbp.
Result shows, L15 is to aflatoxin M 1have good degradation effect, degradation rate is 82.33 ± 0.44%%.
The degradation effect of table 1 Arthrobacter L15 to aflatoxin

Claims (7)

1. a strain Arthrobacter (Arthrobacter sp.) L15, its deposit number is CGMCC No.8869.
2. Arthrobacter L15 claimed in claim 1 and/or its bacteria suspension and/or its fermented liquid and/or the application of its meta-bolites in aflatoxin degradation.
3. Arthrobacter L15 claimed in claim 1 and/or its bacteria suspension and/or its fermented liquid and/or the application of its meta-bolites in preparing the product of aflatoxin degradation.
4. according to the application described in claim 2 or 3, it is characterized in that: described aflatoxin is AFB 1, AFB 2, AFG 1, AFG 2or aflatoxin M 1.
5. a method for aflatoxin degradation, comprises the steps:, with Arthrobacter claimed in claim 1, aflatoxin is carried out to degradation treatment.
6. method according to claim 5, is characterized in that: the mode of described degradation treatment is by the bacteria suspension of Arthrobacter L15 claimed in claim 1 and/or its fermented liquid and/or its meta-bolites and the sample and/or the Product mix that contain aflatoxin.
7. according to the method described in claim 5 or 6, it is characterized in that: described aflatoxin is AFB 1, AFB 2, AFG 1, AFG 2or aflatoxin M 1.
CN201410203045.9A 2014-05-14 2014-05-14 One strain Arthrobacter and the application in aflatoxin degradation thereof Active CN103981132B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410203045.9A CN103981132B (en) 2014-05-14 2014-05-14 One strain Arthrobacter and the application in aflatoxin degradation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410203045.9A CN103981132B (en) 2014-05-14 2014-05-14 One strain Arthrobacter and the application in aflatoxin degradation thereof

Publications (2)

Publication Number Publication Date
CN103981132A true CN103981132A (en) 2014-08-13
CN103981132B CN103981132B (en) 2016-04-06

Family

ID=51273302

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410203045.9A Active CN103981132B (en) 2014-05-14 2014-05-14 One strain Arthrobacter and the application in aflatoxin degradation thereof

Country Status (1)

Country Link
CN (1) CN103981132B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018184538A1 (en) * 2017-04-06 2018-10-11 中国农业科学院油料作物研究所 Pretreatment method for lc-ms detection of aspergillus flavus metabolomics
WO2019105416A1 (en) * 2017-11-29 2019-06-06 中国农业科学院油料作物研究所 Myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin, and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108102971B (en) * 2018-01-26 2021-04-27 山东省花生研究所（山东省农业科学院花生工程技术研究中心） Pseudomonas monteilii capable of resisting heat and degrading aflatoxin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101724590A (en) * 2009-11-16 2010-06-09 西北农林科技大学 Arthrobacter HW08 for degrading swainsonine and application thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101724590A (en) * 2009-11-16 2010-06-09 西北农林科技大学 Arthrobacter HW08 for degrading swainsonine and application thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CSILLA KRIFATON 等: "Analysis of aflatoxin-B1-degrading microbes by use of a combined toxicity-profiling method", 《MUTATION RESEARCH》 *
MATTHEW C.TAYLOR 等: "Identification and characterization of two families of F420H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation", 《MOLECULAR MICROBIOLOGY》 *
WANG R.R.: "KC466276.1", 《GENBANK》 *
计成 等: "黄曲霉毒素生物降解的研究及前景展望", 《中国家禽》 *
陈晓飞 等: "黄曲霉毒素B1（AFB1）降解菌的筛选鉴定", 《河南科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018184538A1 (en) * 2017-04-06 2018-10-11 中国农业科学院油料作物研究所 Pretreatment method for lc-ms detection of aspergillus flavus metabolomics
WO2019105416A1 (en) * 2017-11-29 2019-06-06 中国农业科学院油料作物研究所 Myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin, and application thereof
US11622560B2 (en) 2017-11-29 2023-04-11 Oil Crops Research Institute, Chinese Academy Of Agricultural Sciences Myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and application thereof

Also Published As

Publication number Publication date
CN103981132B (en) 2016-04-06

Similar Documents

Publication Publication Date Title
CN103710292B (en) One Pseudomonas aeruginosa strain and the application in aflatoxin degradation thereof
CN108102963B (en) Bacillus strain for degrading aflatoxin B1 and screening and application thereof
CN109161497B (en) Microbial preparation for degrading aflatoxin and application
CN103981133A (en) Bacillus amyloliquefaciens and application thereof in degradation of zearalenone
CN110564640B (en) Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof
CN110042072B (en) Aflatoxin B degradation1And application thereof
CN101481713B (en) Method for producing aldinamide by biological catalysis of 2-cyano pyrazine and bacterial strain thereof
CN103981135B (en) One strain Sharpe genus bacillus and the application in aflatoxin degradation thereof
CN103981132B (en) One strain Arthrobacter and the application in aflatoxin degradation thereof
CN109393440A (en) A kind of preparation method of the direct putting type compound lactobacillus-fermencucumber agent for pickled cabbage salting
CN108102953A (en) The application of one bacillus subtilis and its Aspergillus ochraceus A that degrades in post-fermented tea pile fermentation
CN103981134B (en) One Pseudomonas aeruginosa strain and the application in degrading zearalenone thereof
CN108294224A (en) A method of it is fermented using lactobacillus plantarum and removes heavy metal cadmium in rice
CN107312719B (en) Aspergillus niger strain W35 and application thereof in degradation of ochratoxin A
CN103614328A (en) Paenibacillus polymyxa producing 2, 3-butanediol and use thereof
CN116064332B (en) Bacterial strain for degrading aflatoxin B1 and application thereof
CN103981131B (en) One strain Methylotrophic bacillus cereus and the application in degrading zearalenone thereof
CN104087523A (en) Marine Streptomyces, method for preparation of Enterocin therefrom and application of the same
CN105670936B (en) A kind of method of Trametes trogii bacterial strain and its application and Pu'er tea processing
CN102643754B (en) Aspergillus oryzae and application thereof in aspect of improving yield of alcohol
CN112646728B (en) Aspergillus awamori and its application in preparing tannase and degrading tannin
CN112725205B (en) Saccharomyces strain and screening method and application thereof
CN105779363A (en) Serratia liquefaciens and method for converting to synthesize heliotropin
CN105349465A (en) Intertidal Exiguobacterium aestuarii and application thereof
CN115927072B (en) High-efficiency degradation aflatoxin B 1 Bacillus megaterium and zearalenone, application thereof and bacterial preparation

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant