CN103977042A - Application of refrigeration technology for drying Ganoderma applanatum - Google Patents
Application of refrigeration technology for drying Ganoderma applanatum Download PDFInfo
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- CN103977042A CN103977042A CN201410204826.XA CN201410204826A CN103977042A CN 103977042 A CN103977042 A CN 103977042A CN 201410204826 A CN201410204826 A CN 201410204826A CN 103977042 A CN103977042 A CN 103977042A
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Abstract
The invention relates to an application of refrigeration technology for drying Ganoderma applanatum, comprising the steps of: refrigerating and stacking newly harvested Ganoderma applanatum for 2-5 days, and drying. The prepared Ganoderma applanatum is high in content of main effective component, namely, polysaccharides and is strong in antitumor activity.
Description
Invention field
The present invention relates to medicine method for making, the particularly application of Refrigeration Technique in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, belongs to medical technical field.
Background technology
Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganodermaapplanatum (Pers.) Pat.) belongs to fungus for basidiomycetes polypor detailed outline Ganoderma applanatum (Pers. Ex Wallr) Pat. section Ganoderma applanatum (Pers. Ex Wallr) Pat..Its another name is redness of the skin or complexion one's old mother bacterium, BIAN tree tongue, flat ebon tongue, flat bacterium, flat sesame, flat cover Ganoderma applanatum (Pers. Ex Wallr) Pat., the rotten bacterium of white macula and Fructus Gleditsia bacterium, among the people being commonly called as " old Hepar Bovis seu Bubali ".Ganoderma applanatum (Pers. Ex Wallr) Pat. is flat, feeble QI, mildly bitter flavor, tool heat clearing away, removing toxic substances, pain relieving, anticancer effect.
Gathering of prior art with processing method be, gather the whole year, dries in the shade or 40～50 DEG C of oven dry.
Summary of the invention
The object of this invention is to provide the dry new method of a kind of Ganoderma applanatum (Pers. Ex Wallr) Pat., the Ganoderma applanatum (Pers. Ex Wallr) Pat. that the method is dry, main effective ingredient polyoses content is high, and active anticancer is strong.
The present invention is achieved in that
The application of Refrigeration Technique in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, is characterized in that: get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, and freezing, the 2-5 days that banks up at dark and damp place is dry.
The application of Refrigeration Technique of the present invention in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, is characterized in that: freezing is to be refrigerated to freeze.
The application of Refrigeration Technique of the present invention in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, is characterized in that: freezing is to be refrigerated to-5 DEG C to freeze below.
The application of Refrigeration Technique of the present invention in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, is characterized in that: dry is that heap postpone is dried or dries or dries.
Of the present inventionly being refrigerated to subzero certain DEG C, is to be refrigerated to and to freeze at this subzero certain DEG C.
So far completed the present invention, the Ganoderma applanatum (Pers. Ex Wallr) Pat. of preparing by drying means of the present invention, main effective ingredient polyoses content is high, and active anticancer is strong.
Further illustrate usefulness of the present invention below by test example, test example is intended to further illustrate usefulness of the present invention, but not limitation of the present invention.
By with a collection of Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, remove impurity, process by the following method.
1) dry: shady and cool ventilation place, spread out and dry;
2) dry: dry;
3) dry: 50 DEG C of following oven dry;
4) the freezing oven dry of banking up: be refrigerated to-5 DEG C to freezing, bank up 3 days at dark and damp place, 50 DEG C of following oven dry.
One, the dry Ganoderma Applanatum Polysaccharides content comparison of distinct methods
The preparation of reference substance solution: get glucose reference substance appropriate, accurately weighed, add water and make the solution of every 1ml containing 0.1mg, to obtain final product.
The preparation of standard curve: precision measures reference substance solution 0.2ml respectively, 0.4m1, 0.6ml, 0.8ml, 1.0ml, 1.2ml, put in 10ml tool plug test tube, add water to 2.0ml, precision adds sulphuric acid anthrone solution, and (precision takes anthrone 0.1g, adding 80% sulfuric acid solution 100ml makes to dissolve, shake up) 6ml, shake up, put in water-bath and heat 15 minutes, take out, put in people's ice bath cooling 15 minutes, taking corresponding reagent as blank, according to " Chinese Pharmacopoeia " ultraviolet visible spectrophotometry, measure absorbance at 625nm wavelength place, taking absorbance as vertical coordinate, concentration is abscissa, drawing standard curve.
The preparation of need testing solution: get the about 2g of each sample powder, accurately weighed, put in apparatus,Soxhlet's, 90ml adds water, electric heater heating and refluxing extraction is to extracting liquid colourless, and extracting solution is transferred in 100ml measuring bottle, adds water to scale, shaking up. precision measures 10ml, adds ethanol 150ml, shakes up, place 12 hours for 4 DEG C, take out, centrifugal, the supernatant that inclines, precipitation is dissolved in water, and is transferred in 50ml measuring bottle, add water to scale, shake up, to obtain final product.
Algoscopy: precision measures need testing solution 2ml, put in 10ml tool plug test tube, method under the preparation of sighting target directrix curve, from " precision adds sulphuric acid anthrone solution 6ml ", measure absorbance in accordance with the law, read the weight (mg) containing glucose in need testing solution from standard curve, calculate, to obtain final product.
Press dry product and calculate, in anhydrous glucose (C6H12O6), the results are shown in Table 1 containing Ganoderma Applanatum Polysaccharides.
Table 1 distinct methods is dried Ganoderma Applanatum Polysaccharides content (%)
| Drying means | Dry | Dry | Dry | The freezing oven dry of banking up |
| Content (%) | 4.26 | 4.25 | 4.25 | 6.73 |
Found out by table 1, Ganoderma applanatum (Pers. Ex Wallr) Pat. " freezing heap postpone is dry ", polyoses content is apparently higher than " drying ", " drying " and " oven dry ".
Two, the dry anti-human lung of Ganoderma applanatum (Pers. Ex Wallr) Pat. source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and the people's chronic myelogenous leukemia cell K562 test of distinct methods.
1, prepare Ganoderma applanatum (Pers. Ex Wallr) Pat. extract and get respectively the dry Ganoderma applanatum (Pers. Ex Wallr) Pat. pulverizing of distinct methods, respectively decoct with water twice, add water for the first time 8 times and measure, decoct 2 hours, add water for the second time 8 times and measure, decoct 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, and being concentrated into relative density is 1.10(60 DEG C) time, add ethanol to make to reach 75% containing alcohol amount, precipitation, leaves standstill 36 hours, draw supernatant, reclaim ethanol, concentrated, dry, pulverize, obtain the dry Ganoderma applanatum (Pers. Ex Wallr) Pat. extract of distinct methods.
2, tumor cell line people lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562, use RPMI-1649 culture fluid, and 37 DEG C, 5%CO2, relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.
3, the inhibitory action of method MTT colorimetric method for determining extract to growth of tumour cell.The trophophase cell of taking the logarithm, is mixed with cell suspension, 0.8 × 105/ml of 1 × 105/ml of suspension cell attached cell with fresh RPMI-1640 culture fluid.Suspension cell is inoculated in 96 well culture plates after adding respectively variable concentrations Experimental agents; Attached cell is first inoculated in 96 well culture plates, after every hole 90 μ l24h, adds respectively variable concentrations Experimental agents.Final volume is that every hole 100 μ l and every group are established 3 parallel holes, establishes altogether 4 groups: be subject to reagent group T (culture fluid+cell suspension+variable concentrations is subject to reagent), negative control group N (culture fluid+cell suspension), medicine color matched group TC (culture fluid+variable concentrations is subject to reagent), blank group B (culture fluid+normal saline).Tested concentration is followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 DEG C, in 5%CO2 incubator, cultivate after 72h, after adding the 10 μ l concussions of MTT solution to mix to every hole, continue to cultivate 4h, add SDS90 μ l to stop cultivating, 37 DEG C are spent the night, and then under room temperature, on micro oscillator, shake 10min, microplate reader is measured the absorbance (OD value) at 570nm wavelength place, and experiment repeats 3 times.
Calculate as follows growth inhibition ratio:
Growth inhibition ratio (%)=(N group OD average-B group OD average)-(T group OD average-TC group OD average)/N group OD average-B group OD average × 100%.
4, different extracts to the suppression ratio of people's lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 the results are shown in Table 2, table 3, table 4.
The suppression ratio result of table 2 to SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 47.43 | 58.36 | 67.11 |
| Dry | 47.42 | 58.37 | 67.12 |
| Dry | 47.42 | 58.35 | 67.12 |
| The freezing oven dry of banking up | 53.27 | 73.42 | 81.44 |
The suppression ratio result of table 3 to HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 46.72 | 56.54 | 65.57 |
| Dry | 46.41 | 56.51 | 65.58 |
| Dry | 46.34 | 56.53 | 65.54 |
| The freezing oven dry of banking up | 57.61 | 73.37 | 86.18 |
The suppression ratio result of table 4 to K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 53.28 | 66.51 | 71.62 |
| Dry | 53.27 | 66.52 | 71.60 |
| Dry | 53.26 | 66.50 | 71.56 |
| The freezing oven dry of banking up | 61.73 | 74.35 | 80.47 |
Result shows, the dry Ganoderma applanatum (Pers. Ex Wallr) Pat. of distinct methods all has inhibitory action to the propagation of SPCA-1, HepG2, K562 cell, wherein, freezingly banks up that to dry curative effect best.
Detailed description of the invention
embodiment 1
Get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, put in freeze dryer, be refrigerated to-5 DEG C to freezing, banking up 2 days in dark and damp place, dries.
embodiment 2
Get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, put in freeze dryer, be refrigerated to-10 DEG C, bank up 3 days at dark and damp place, dry.
embodiment 3
Get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, put in freeze dryer, be refrigerated to-20 DEG C, bank up in the cool 4 days, dry.
embodiment 4
Get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, put in freeze dryer, be refrigerated to-32 DEG C to freezing, bank up 3 days outdoor, dry.
embodiment 6
Get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, put in freeze dryer, be refrigerated to-15 DEG C, bank up 5 days indoor, dry.
Claims (4)
1. the application of Refrigeration Technique in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry, is characterized in that: get the Ganoderma applanatum (Pers. Ex Wallr) Pat. of newly gathering, and freezing, the 2-5 days that banks up is dry.
2. the application in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry according to the Refrigeration Technique of claim 1, is characterized in that: freezing is to be refrigerated to freeze.
3. the application in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry according to the Refrigeration Technique of claim 1, is characterized in that: freezing is to be refrigerated to-5 DEG C to freeze below.
4. the application in Ganoderma applanatum (Pers. Ex Wallr) Pat. is dry according to the Refrigeration Technique of claim 1, is characterized in that: dry is that heap postpone is dried or dries or dries.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5366412A (en) * | 1976-10-30 | 1978-06-13 | Sato Akihiko | Extracting and separating effective component of mushroom *1mannentake1* |
| CN1382593A (en) * | 2002-06-05 | 2002-12-04 | 张兴范 | Ganoderma-shaped wood artwork and its making method |
| CN103055022A (en) * | 2013-01-10 | 2013-04-24 | 济南康众医药科技开发有限公司 | Method for processing rehmannia and application for rehmannia in preparation for medicine |
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2014
- 2014-05-15 CN CN201410204826.XA patent/CN103977042A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5366412A (en) * | 1976-10-30 | 1978-06-13 | Sato Akihiko | Extracting and separating effective component of mushroom *1mannentake1* |
| CN1382593A (en) * | 2002-06-05 | 2002-12-04 | 张兴范 | Ganoderma-shaped wood artwork and its making method |
| CN103055022A (en) * | 2013-01-10 | 2013-04-24 | 济南康众医药科技开发有限公司 | Method for processing rehmannia and application for rehmannia in preparation for medicine |
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