CN103969305B - bipolar electrode electrochemical luminescence imaging electrolytic cell - Google Patents
bipolar electrode electrochemical luminescence imaging electrolytic cell Download PDFInfo
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- CN103969305B CN103969305B CN201410182107.2A CN201410182107A CN103969305B CN 103969305 B CN103969305 B CN 103969305B CN 201410182107 A CN201410182107 A CN 201410182107A CN 103969305 B CN103969305 B CN 103969305B
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- electrolytic cell
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Abstract
A kind of bipolar electrode electrochemical luminescence imaging electrolytic cell, the upper surface of subtegulum stick with glue be connected in substrate, the upper surface of middle substrate sticks with glue and is connected to substrate, and upper substrate, middle substrate, subtegulum are lucite sheet.It is machined with electrolytic cell c in the center of upper substrate, the end of electrolytic cell c is middle substrate, it is machined with passage b in the center of upper substrate and middle substrate, the end of passage b is subtegulum, left end at upper substrate and middle substrate upper channel b is machined with left reservoir a, right-hand member is machined with right reservoir d, and passage b and left reservoir a and right reservoir d is connected.The present invention have reasonable in design, simple in construction, reusable, just put the advantages such as general with inverted microscope, can be applicable in bipolar electrode electrochemical luminescence imaging analysis.
Description
Technical field
The invention belongs to electrochemical luminescence equipment or device technique field, be specifically related to utilize the electric field of bipolar electrode
Enrichment effect, field inducing electrochemiluminescence electrolytic cell.
Background technology
Bipolar electrode is one and is not connected with external power and is placed in and drives leading in electrolyte between negative electrode and anode
Body.When a sufficiently strong electric field puts on electrolyte solution, even if this conductor is with external power the most directly
Electrical connection, faraday reaction also can occur at the two ends of conductor simultaneously, wherein near drive negative electrode one end send out
, there is reduction reaction, referred to as negative electrode near the one end driving anode in raw oxidation reaction, referred to as anode.Electrochemistry
Luminescence be apply on electrode certain voltage make between electrode reaction product or in electrode reaction product and solution certain
A kind of light radiation carrying out chemical reaction between component and produce.Luminescence reagent is added, as ruthenium joins in electrolytic cell
Pyridine-tripropyl amine (TPA), luminol-hydrogen peroxide etc., driving electrode two ends to apply voltage, then can be at bipolarity electricity
One end of pole detects luminous signal.Traditional bipolar electrode passes through standard photolithography directly at the base of electrolytic cell
Being made on plate, channel die prepares with standard photolithography too, then through cast dimethyl silicone polymer
With coagulator, be heating and curing, the demoulding, it is carried out four steps of irreversible encapsulation with bipolar electrode substrate,
I.e. can get the entirety of bipolar electrode and the sealing of dimethyl silicone polymer electrolytic cell passage.This legal system makees complexity,
And due to bipolar electrode easy damaged, electrolytic cell passage recycling rate of waterused is low.
Summary of the invention
The technical problem to be solved is to overcome above-mentioned traditional bipolar electrode electrochemical cell
Shortcoming, it is provided that a kind of reasonable in design, bipolar electrode electrochemical luminescence imaging electrolytic cell of simple in construction.
Solve above-mentioned technical problem and be the technical scheme is that substrate in the upper surface of subtegulum is provided with,
The upper surface of middle substrate is provided with substrate, is machined with electrolytic cell c, electrolytic cell c in the center of upper substrate
The end be middle substrate, be machined with passage b in the center of upper substrate and middle substrate, the end of passage b is lower base
Sheet, the left end at upper substrate and middle substrate upper channel b is machined with left reservoir a, right-hand member is machined with right reservoir d,
Passage b and left reservoir a and right reservoir d is connected.
The electrolytic cell c of the present invention is shaped as square electrolytic cell groove or rectangle electrolytic cell groove；Passage b
Be shaped as rectangular recess, the length of side of the long length of side ＞ square indentations of rectangular recess, square indentations
The broadside of length of side ＞ rectangular recess is long.The left reservoir a of the present invention, right reservoir d, the center line of passage b
Coincide with the horizontal center line of electrolytic cell c.
The groove generally circular in shape of the left reservoir a of the present invention, the width of the diameter ＞ passage b of circular groove, right
The shape of reservoir d is identical with the shape of left reservoir a.
The length of side of the square electrolytic cell groove of the present invention is 6～9mm, rectangle electrolytic cell groove length × a width of 6
×9.A length of 16～22mm, a width of 2～the 3mm of the passage b of the present invention.The left reservoir a of the present invention and the right side
A diameter of the 3 of reservoir d～5mm.
The length of side most preferably 8.5mm of the square electrolytic cell of the present invention.The passage b's of the present invention is the most a length of
20mm, a width of 3mm.The left reservoir a of the present invention, the optimum diameter of right reservoir d are 4mm.
Owing to the electrolytic cell of the present invention is separate with bipolar electrode, reusable, nontransparent material bipolar
Property electrode (such as gold, silver etc.), electrod-array faces up, with just put microscope from top detect；There iing step
In the case of, electrode surface will not touch at the bottom of pond down, detects from below with inverted microscope, extends electrolytic cell
The scope of application.The present invention have reasonable in design, simple in construction, reusable, just put and inverted microscope
The advantage such as general, can be applicable in bipolar electrode electrochemical luminescence imaging analysis.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention 1.
Fig. 2 is the A-A sectional view of Fig. 1.
Detailed description of the invention
The present invention is described in more detail with embodiment below in conjunction with the accompanying drawings, but the invention is not restricted to following
Embodiment.
Embodiment 1
As shown in Figure 1, 2, the bipolar electrode electrochemical luminescence imaging electrolytic cell of the present embodiment by upper substrate 1,
Middle substrate 2, subtegulum 3 couple composition.
The upper surface of subtegulum 3 stick with glue be connected in substrate 2, the upper surface of middle substrate 2 sticks with glue and is connected to
Substrate 1, upper substrate 1, middle substrate 2, subtegulum 3 are lucite sheet.Add in the center of upper substrate 1
Work has electrolytic cell c, the structure of electrolytic cell c to be square indentations, and the end of square indentations is middle substrate 2, square
The length of side of connected in star is 8.5mm, is machined with passage b, passage b in the center of upper substrate 1 and middle substrate 2
Be shaped as rectangular recess, the end of rectangular recess is subtegulum 3, a length of 20mm of rectangular recess, width
For 3mm, passage b for filling the electrochemical luminescence reagent of detection, logical on upper substrate 1 and middle substrate 2
The left end of road b is machined with left reservoir a, right-hand member is machined with right reservoir d, left reservoir a and right reservoir d
Groove generally circular in shape, a diameter of 4mm, the end of circular groove is subtegulum 3.Passage b respectively with left liquid storage
Groove a and right reservoir d is connected, left reservoir a, right reservoir d, center line and the electrolytic cell c of passage b
Horizontal center line coincide, electrolytic cell c for installing the glass substrate of bipolar electrode 4, bipolar electrode 4
Extensively apply in electrochemistry and report at document.
Embodiment 2
The structure of electrolytic cell c is square indentations, and the length of side of square indentations is 6mm, and passage b is shaped as
Rectangular recess, a length of 16mm of rectangular recess, a width of 2mm, the groove generally circular in shape of left reservoir a,
A diameter of 4mm of circular groove, the structure of right reservoir d is identical with left reservoir a.Other parts
And the connecting relation of parts is same as in Example 1.
Embodiment 3
The structure of electrolytic cell c is square indentations, and the length of side of square indentations is 9mm, and passage b is shaped as
Rectangular recess, a length of 22mm of rectangular recess, a width of 3mm, the groove generally circular in shape of left reservoir a,
A diameter of 5mm of circular groove, the structure of right reservoir d is identical with left reservoir a.Other parts
And the connecting relation of parts is same as in Example 1.
Embodiment 4
In embodiment 1, the structure of electrolytic cell c is rectangular recess, a length of 9mm of rectangular recess, width
For 6mm, the center line of electrolytic cell c length direction and left reservoir a, right reservoir d, the center line of passage b
Coincide.The annexation of other parts and parts is identical with corresponding embodiment.
Claims (5)
1. a bipolar electrode electrochemical luminescence imaging electrolytic cell, is provided with upper substrate on subtegulum (3)
(1), upper substrate (1) is machined with the left storage that electrolytic cell (c), passage (b) and passage (b) are connected
Liquid bath (a) and right reservoir (d), be provided with bipolar electrode 4 in electrolytic cell (c), it is characterised in that:
Being provided with middle substrate (2) between upper substrate (1) and subtegulum (3), described electrolytic cell (c) is positioned at
The center of substrate (1), the end of electrolytic cell (c) is middle substrate (2)；Described passage (b) processing exists
Upper substrate (1) and the center of middle substrate (2), the end of passage (b) is subtegulum (3)；A described left side
Reservoir (a) and right reservoir (d) are processed on upper substrate (1) and middle substrate (2), left reservoir (a)
It is subtegulum (3) with the end of right reservoir (d).
Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 1, it is characterised in that:
Described electrolytic cell (c) be shaped as square electrolytic cell groove or rectangle electrolytic cell groove；Described is logical
Road (b) be shaped as rectangular recess, the length of side of the long length of side ＞ square indentations of rectangular recess, square
The broadside of the length of side ＞ rectangular recess of groove is long；Described left reservoir (a), right reservoir (d), logical
The center line in road (b) coincides with the horizontal center line of electrolytic cell (c).
Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 1, it is characterised in that:
The groove generally circular in shape of described left reservoir (a), the width of diameter ＞ passage (b) of circular groove, right
The shape of reservoir (d) is identical with the shape of left reservoir (a).
Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 2, it is characterised in that:
Described square electrolytic cell is recessed+and the length of side of groove is 6～9mm, rectangle electrolytic cell groove length × a width of 6 × 9；
A length of the 16 of described passage (b)～22mm, a width of 2～3mm；Described left reservoir (a) and right liquid storage
A diameter of the 3 of groove (d)～5mm.
Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 2, it is characterised in that:
The length of side of described square electrolytic cell is 8.5mm；A length of 20mm of described passage (b), a width of 3mm；
Described left reservoir (a), a diameter of 4mm of right reservoir (d).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410182107.2A CN103969305B (en) | 2014-04-30 | 2014-04-30 | bipolar electrode electrochemical luminescence imaging electrolytic cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410182107.2A CN103969305B (en) | 2014-04-30 | 2014-04-30 | bipolar electrode electrochemical luminescence imaging electrolytic cell |
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| CN103969305A CN103969305A (en) | 2014-08-06 |
| CN103969305B true CN103969305B (en) | 2016-08-17 |
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| CN201410182107.2A Expired - Fee Related CN103969305B (en) | 2014-04-30 | 2014-04-30 | bipolar electrode electrochemical luminescence imaging electrolytic cell |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093015A (en) * | 2016-06-01 | 2016-11-09 | 华南师范大学 | A kind of closed type bipolar electrode electrogenerated chemiluminescence cloth chip and preparation method thereof and purposes |
| CN108088879B (en) * | 2017-11-30 | 2020-02-18 | 华南理工大学 | Universal two-electrode type modified electrode unit and preparation method and application thereof |
| JP6757079B2 (en) * | 2018-08-24 | 2020-09-16 | 国立大学法人東北大学 | Electrochemical sensor and manufacturing method of electrochemical sensor |
| CN109324037A (en) * | 2018-10-09 | 2019-02-12 | 济南大学 | The building of alpha-fetoprotein bipolar electrode paper chip Electrochemiluminescsensor sensor |
| CN110412021B (en) * | 2019-08-06 | 2022-04-29 | 华南师范大学 | Electrochemical luminescence micro-fluidic chip sharing bipolar electrode cathode and application thereof |
| CN114487055B (en) * | 2022-01-25 | 2024-02-06 | 华南师范大学 | Multipath closed bipolar electrochemical luminescence chip and application thereof in detection sensing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201016954Y (en) * | 2007-03-02 | 2008-02-06 | 厦门大学 | Static ECL electrolytic cell adapted for lamina electrode |
| CN102749323A (en) * | 2012-07-14 | 2012-10-24 | 福州大学 | Electrochemiluminescence imaging system |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507708A (en) * | 2011-10-21 | 2012-06-20 | 合肥工业大学 | Chip-type thin-layer electrolytic cell capillary electrophoresis microfluidic online sample feeding device |
| CN102636530A (en) * | 2012-03-23 | 2012-08-15 | 厦门大学 | Microelectrode array electrolyzer for in-situ cell electrochemical and microscopic detection |
| CN203247315U (en) * | 2013-04-21 | 2013-10-23 | 武汉兴达高技术工程有限公司 | Bipolar electrolysis bath |
| CN203929704U (en) * | 2014-04-30 | 2014-11-05 | 陕西师范大学 | Bipolar electrode electrochemiluminescence imaging electrolytic cell |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201016954Y (en) * | 2007-03-02 | 2008-02-06 | 厦门大学 | Static ECL electrolytic cell adapted for lamina electrode |
| CN102749323A (en) * | 2012-07-14 | 2012-10-24 | 福州大学 | Electrochemiluminescence imaging system |
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