CN103961716A - Production method of water-soluble soy isoflavone - Google Patents
Production method of water-soluble soy isoflavone Download PDFInfo
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- CN103961716A CN103961716A CN201410194538.0A CN201410194538A CN103961716A CN 103961716 A CN103961716 A CN 103961716A CN 201410194538 A CN201410194538 A CN 201410194538A CN 103961716 A CN103961716 A CN 103961716A
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- soybean isoflavone
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- schardinger dextrin
- soy isoflavone
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Abstract
A production method of water-soluble soy isoflavone comprises the following steps: a, crystallizing the soy isoflavone, collecting crystals, drying, and crushing; b, preparing a beta-cyclodextrin saturated aqueous solution; c, preparing a soy isoflavone alcohol solution; d, slowly adding the soy isoflavone alcohol solution into the beta-cyclodextrin saturated aqueous solution, and keeping the temperature at 60-90 DEG C, and continuing stirring for 0.5-3 h; e, cooling, and collecting, so that soy isoflavone crystals embedded by the beta-cyclodextrin are obtained; f, crushing the crystals to obtain the water-soluble soy isoflavone. The production method has the advantages as follows: the produced water-soluble soy isoflavone is water-soluble, clear and transparent, slightly sweet in taste and free of beany flavor, bitter taste and other bad smell and taste; the application field of the soy isoflavone is greatly broadened.
Description
Technical field
The present invention relates to the production of water-soluble soybean isoflavone, be specifically related to a kind of production method of water-soluble soybean isoflavone.
Background technology
at present, soybean isoflavone is mainly present in various leguminous plants, and Semen sojae atricolor is the topmost dietary source of soybean isoflavone.Soybean isoflavone has the molecular structure similar to estrogen, can with women's body in estrogen competitive binding estrogen receptor, thereby reach the effect of two-ways regulation women body inner estrogen level.Research shows, soybean isoflavone has more outstanding effect at aspects such as improving Women’s climacteric, osteoporosis, cardiovascular disease.
Common soybeans isoflavone is insoluble in water, has the gas beastly such as beany flavor, bitter taste, flavour, has limited its application in fields such as functional drinks, instant solid beverage, cosmetics.The patent No. is 6458406 United States Patent (USP), and its production method taking common soybeans isoflavone as raw material, through heating, with flocculating agent flocculations such as calcium chloride, is removed water-fast composition in common soybeans isoflavone for directly, makes water-soluble soybean isoflavone.The patent No. is 6340470 United States Patent (USP), and its production method taking common soybeans isoflavone as raw material, through heating, adding the cosolvent hydrotropies such as propylene glycol, makes water-soluble soybean isoflavone for directly.The weak point of above two kinds of methods is, all directly taking common soybeans isoflavone as raw material, beany flavor and the bitter taste of not taking measures to remove common soybeans isoflavone, the water-soluble soybean isoflavone making still has beany flavor beastly and bitter taste, has limited the application of its product.
Publication number is the Chinese patent of CN102586365A, and its production method, for to turn glycosylation between maltogenic amylase catalysis soybean isoflavone glucoside and glycosyl donor, makes the soybean isoflavone product of highly-water-soluble.The weak point of the method is, has changed the original molecular structure of soybean isoflavone, the safety checking of still needing.
Summary of the invention
Object of the present invention is exactly the deficiency for current said method, and a kind of production method of water-soluble soybean isoflavone is provided.
The present invention includes following steps:
A, soybean isoflavone is dissolved in to concentration is in 30%～50% alcoholic solution, fully stirs 0.5h～2h at 30 DEG C～60 DEG C, and cooling, standing sedimentation or filtration, collect crystalline solid, dries, and grinds;
B, beta-schardinger dextrin-is dissolved in purified water, beta-schardinger dextrin-saturated aqueous solution is made in heating;
C, be in 60%～90% alcoholic solution by being dissolved in concentration through the crystal grinding in step a, filter, make soybean isoflavone alcoholic solution;
D, under stirring condition, soybean isoflavone alcoholic solution is slowly added in beta-schardinger dextrin-saturated aqueous solution, and is incubated 60 DEG C～90 DEG C, continue stir 0.5h～3h, make mixed liquor;
E, mixed liquor is cooled to 0 DEG C～10 DEG C, crystallization, standing sedimentation or filtration, collect crystalline solid, and the ethanol wash crystallization body that is 60%～90% with concentration, then standing sedimentation or filtration are collected to such an extent that pass through the soybean isoflavone crystalline solid of beta-schardinger dextrin-embedding;
F, by the soybean isoflavone crystalline solid vacuum drying through beta-schardinger dextrin-embedding of finally collecting, baking temperature is 60 DEG C～90 DEG C, vacuum is 0.02MPa～0.06MPa, grinds and obtain water-soluble soybean isoflavone.
2, the production method of a kind of water-soluble soybean isoflavone according to claim 1, is characterized in that in b step, beta-schardinger dextrin-is dissolved in purified water, and heating-up temperature is 60 DEG C～90 DEG C.
Advantage of the present invention is: the water-soluble soybean isoflavone that the present invention produces, and water-soluble clear, taste is micro-sweet, and bad gas, the flavours such as free from beany flavor and bitter taste have been widened the application of soybean isoflavone greatly.
Detailed description of the invention
The present invention includes following steps:
A, soybean isoflavone is dissolved in to concentration is in 30%～50% alcoholic solution, fully stirs 0.5h～2h at 30 DEG C～60 DEG C, and cooling, standing sedimentation or filtration, collect crystalline solid, dries, and grinds;
B, beta-schardinger dextrin-is dissolved in purified water, beta-schardinger dextrin-saturated aqueous solution is made in heating;
C, be in 60%～90% alcoholic solution by being dissolved in concentration through the crystal grinding in step a, filter, make soybean isoflavone alcoholic solution;
D, under intense agitation, soybean isoflavone alcoholic solution is slowly added in beta-schardinger dextrin-saturated aqueous solution, and is incubated 60 DEG C～90 DEG C, continue stir 0.5h～3h, make mixed liquor;
E, be cooled to 0 DEG C～10 DEG C, crystallization, standing sedimentation or filtration, collect crystalline solid, the ethanol wash crystallization body that is 60%～90% with concentration, standing sedimentation or filtration, collect to such an extent that pass through the soybean isoflavone crystalline solid of beta-schardinger dextrin-embedding;
F, by the soybean isoflavone crystalline solid vacuum drying through beta-schardinger dextrin-embedding of finally collecting, baking temperature is 60 DEG C～90 DEG C, vacuum is 0.02MPa～0.06MPa, grinds and obtain water-soluble soybean isoflavone.
2, the production method of a kind of water-soluble soybean isoflavone according to claim 1, is characterized in that in b step, beta-schardinger dextrin-is dissolved in purified water, and heating-up temperature is 60 DEG C～90 DEG C.
Specific implementation method 1:
Get 20g common soybeans isoflavone (content 40%), add 100mL50% ethanol, be heated to 50 DEG C, fully stir 1h, be cooled to sucking filtration after room temperature.The crystallization soybean isoflavone obtaining is dried under vacuum condition, grind.The crystallization soybean isoflavone of grinding is dissolved in 50mL80% ethanol, slowly joins while stirring in the beta-schardinger dextrin-saturated aqueous solution at 1000mL60 DEG C for preparing in advance, continue to stir 2h.Be cooled to 5 DEG C, leave standstill 24 hours, sucking filtration, is that 80% ethanol washs by the concentration of 2 times of volumes, sucking filtration again.Filter cake is dried at 80 DEG C of vacuum 0.02MPa, temperature, grind, obtain the milky water-soluble soybean isoflavone of 55g, content 10.65%, dissolubility is 1.5g/100mL water.
Specific implementation method 2:
Get 25kg common soybeans isoflavone (content 40%), add 80L40% ethanol, be heated to 60 DEG C, fully stir 1h, be cooled to sucking filtration after room temperature.The crystallization soybean isoflavone obtaining is dried under vacuum condition, grind.The crystallization soybean isoflavone of grinding is dissolved in 60L75% ethanol, slowly joins while stirring in the beta-schardinger dextrin-saturated aqueous solution at 60L80 DEG C for preparing in advance, continue to stir 2h.Be cooled to 5 DEG C, leave standstill 24 hours, sucking filtration, is that 75% ethanol washs by the concentration of 2 times of volumes, sucking filtration again.Filter cake is dried at 80 DEG C of vacuum 0.02MPa, temperature, grind, obtain the milky water-soluble soybean isoflavone of 65kg, content 11.76%, dissolubility is 1.2g/100mL water.
Claims (2)
1. a production method for water-soluble soybean isoflavone, is characterized in that comprising the steps:
A, soybean isoflavone is dissolved in to concentration is in 30%～50% alcoholic solution, fully stirs 0.5h～2h at 30 DEG C～60 DEG C, and cooling, standing sedimentation or filtration, collect crystalline solid, dries, and grinds;
B, beta-schardinger dextrin-is dissolved in purified water, beta-schardinger dextrin-saturated aqueous solution is made in heating;
C, be in 60%～90% alcoholic solution by being dissolved in concentration through the crystal grinding in step a, filter, make soybean isoflavone alcoholic solution;
D, under stirring condition, soybean isoflavone alcoholic solution is slowly added in beta-schardinger dextrin-saturated aqueous solution, and is incubated 60 DEG C～90 DEG C, continue stir 0.5h～3h, make mixed liquor;
E, mixed liquor is cooled to 0 DEG C～10 DEG C, crystallization, standing sedimentation or filtration, collect crystalline solid, and the ethanol wash crystallization body that is 60%～90% with concentration, then standing sedimentation or filtration are collected to such an extent that pass through the soybean isoflavone crystalline solid of beta-schardinger dextrin-embedding;
F, by the soybean isoflavone crystalline solid vacuum drying through beta-schardinger dextrin-embedding of finally collecting, baking temperature is 60 DEG C～90 DEG C, vacuum is 0.02MPa～0.06MPa, grinds and obtain water-soluble soybean isoflavone.
2. the production method of a kind of water-soluble soybean isoflavone according to claim 1, is characterized in that in b step, beta-schardinger dextrin-is dissolved in purified water, and heating-up temperature is 60 DEG C～90 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410194538.0A CN103961716B (en) | 2014-05-09 | 2014-05-09 | A kind of production method of water-soluble soybean isoflavone |
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| CN201410194538.0A CN103961716B (en) | 2014-05-09 | 2014-05-09 | A kind of production method of water-soluble soybean isoflavone |
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| CN103961716A true CN103961716A (en) | 2014-08-06 |
| CN103961716B CN103961716B (en) | 2016-09-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105381473A (en) * | 2015-12-23 | 2016-03-09 | 陕西源邦生物技术有限公司 | Preparation method of water-soluble soybean isoflavone |
| CN108203424A (en) * | 2017-11-20 | 2018-06-26 | 荆门市德爱生物工程股份有限公司 | A kind of production method for the high-purity soybean isoflavone for removing bitter taste |
| CN109105669A (en) * | 2018-09-20 | 2019-01-01 | 浦江县酉泽水产科技有限公司 | Improve the preparation method of Tilapia mossambica immunity feed addictive |
-
2014
- 2014-05-09 CN CN201410194538.0A patent/CN103961716B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 李彦军等: ""大豆异黄酮苷元β-环糊精微胶囊的制备和鉴定"", 《食品工业科技》, vol. 30, no. 9, 31 December 2009 (2009-12-31), pages 183 - 185 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105381473A (en) * | 2015-12-23 | 2016-03-09 | 陕西源邦生物技术有限公司 | Preparation method of water-soluble soybean isoflavone |
| CN108203424A (en) * | 2017-11-20 | 2018-06-26 | 荆门市德爱生物工程股份有限公司 | A kind of production method for the high-purity soybean isoflavone for removing bitter taste |
| CN109105669A (en) * | 2018-09-20 | 2019-01-01 | 浦江县酉泽水产科技有限公司 | Improve the preparation method of Tilapia mossambica immunity feed addictive |
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| CN103961716B (en) | 2016-09-07 |
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Granted publication date: 20160907 |