CN103931392A - Cutting propagation method for ornithogalum caudatum ait bulb - Google Patents

Abstract

The invention belongs to the fields of plant cultivation techniques, landscape plants and ornamental horticulture and relates to a cutting propagation method for an ornithogalum caudatum ait bulb. In the technical scheme, turfy soil, perlite and vermiculite are mixed according to a certain proportion to form a cultivation medium for cutting propagation of the ornithogalum caudatum ait bulb, and differentiation growing and the reproductive rate of the cut ornithogalum caudatum ait bulb sections are improved by means of the technical conditions and methods of adjusting the suitable PH value of the medium, providing suitable cultivating temperature and humidity and the like. According to the cutting propagation method for the ornithogalum caudatum ait bulb, the cultivation medium for the cutting propagation is optimized, so that the cut ornithogalum caudatum ait bulb sections are in an excellent growing environment, differentiation growing and the reproductive rate of the cut ornithogalum caudatum ait bulb sections can be effectively improved, and the simple and practical technology for producing ornithogalum caudatum ait seedlings is provided for vast flower production enterprises.

Description

A kind of cutting propagation method of tiger's-eye evergreen bulb cutting

technical field

The invention relates to a method for cutting and propagating a tiger's-eye evergreen bulb. The invention belongs to the fields of plant cultivation technology, garden plants and ornamental gardening.

Background technique

Evergreen tiger eye, also known as squill, bird's milk flower. It is a perennial herb of the Liliaceae evergreen genus, native to southern Africa. Bulbs ovoid, green, up to 10 cm in diameter. Leaves 5-6, band-like or strip-shaped-lanceolate, 30-60 cm long, 2.5-5 cm wide. As early as the 1970s, tiger-eye evergreen was introduced to China as an ornamental plant, and it was planted in Hainan, Xi'an, and Changbai Mountains. After the experimental research of many scholars, it was found that Dieffenbachia saponin (OSW-1 for short) was isolated from Dieffenbachia tiger's eye. In the physiological activity screening experiment, it was found that the anticancer activity of "OSW-1" in vitro is ten times or even a hundred times stronger than that of paclitaxel, doxorubicin, camptothecin and other anticancer drugs in clinical use. And it is not toxic to normal cells. The saponin OSW-1 isolated from Dieffenbachia tiger's eye has obvious anticancer activity on lung cancer and breast cancer, which is 10 to 100 times the anticancer activity of common anticancer drugs camptothecin, doxorubicin and paclitaxel. This astonishing discovery will mean that Dieffenbachia tiger's eye may replace drugs such as paclitaxel, doxorubicin, and camptothecin. The economic value is very huge, and it also has high ornamental value as an ornamental flower. At present, the breeding method of evergreen is mainly divided into bulbs and sowing. Although it is relatively easy to survive, a mature bulb can be divided into less than 10 bulbs per year, and the reproductive efficiency is very low. The emergence rate of sowing and breeding is not high. Long, about 3 years or so, greatly affects production efficiency. The cutting propagation method has the advantages of simple operation, high survival rate, and low production cost. It is easily accepted by the market and produces good technical effects. This method is very suitable for popularization and application.

Contents of the invention

The object of the present invention is to aim at the deficiencies in the prior art and provide a method for cutting and propagating Dietera tiger's eye bulbs, which is simple and practical, has low cost, is convenient for large-scale artificial cultivation, and can quickly and effectively mass-produce high-quality Dieffenbachia tiger's eye seedlings.

In order to achieve such purpose, in the technical scheme of the present invention, a certain proportion of peat soil, perlite and vermiculite are used to mix to form the culture medium for the cutting propagation of Dieffenbachia tiger's-eye bulb slices, and by adjusting the pH of the suitable matrix, suitable cultivation conditions are provided. Technical conditions such as temperature and humidity can improve the differentiation, growth and reproduction rate of cuttage Dieffenbachia tiger's eye bulb slices.

The purpose of the present invention is achieved through the following technical solutions.

1. Preparation of culture medium: take dry peat soil, perlite and vermiculite according to the ratio of 3:1:1 to form a culture medium, and then put it in an autoclave to sterilize at 121°C for 30-50 minutes;

2. Adjustment of the pH value of the culture medium: Add appropriate amount of water to the culture medium to make it moist, adjust the pH of the culture medium to 6.0-6.5, then pour the culture medium into the seedling tray for later use, adjust the pH value of the culture medium with dilute Phosphoric acid, sodium hydroxide solution;

3. Cutting and disinfection of the bulb of the mother plant: take the evergreen cue ball with a circumference greater than 20cm, clean it, remove the outer layer of dry scales, put the bulb plate upwards, and cut the cue ball longitudinally from top to bottom into 16 to 20 pieces , and ensure that each part has a bulb disc, and then soak these cut slices of evergreen bulbs in carbendazim diluted 500 to 1000 times for 30 to 40 minutes for disinfection, and then dip the cut surface in rooting powder powder Once, the medicinal powder is coated on the cut surface of the bulb disc, and then taken out as cuttings to be inserted;

4. Planting of bulb slices and cuttings: insert the sterilized cuttings into the culture medium in the seedling tray, the planting row spacing is 4×4 cm, and insert the bulb slices into the culture medium with the direction of the bulb plate, 2/3 of the cuttings Expose the culture substrate, place it in a dark condition at 22-25°C for 70-90 days, control the humidity between 70% and 85% during the period, and water it thoroughly after each culture substrate is dry;

5. Transplantation of seedlings: When new root buds grow on the bulb slices of the cuttings, transplant them into a culture medium mixed with vinegar grains, humus and perlite at a ratio of 6:1:1, and grow under conventional management conditions. into normal tiger eye evergreen plants.

The method for cutting and propagating the evergreen bulbs of the present invention is simple and convenient to operate. By optimizing the culture medium for cutting propagation, the cut evergreen bulb slices are placed in an excellent growth environment, and the differentiation of the evergreen bulb slices cut can be effectively improved. growth and reproduction rate.

The method of the invention can quickly and effectively mass-produce high-quality evergreen seedlings, and provides a simple and practical technology for producing evergreen seedlings for flower production enterprises.

Detailed ways

The technical solutions of the present invention are further described in the following embodiments. The following examples are not intended to limit the present invention.

Example 1

Select a cue ball with a diameter of 22 centimeters and carry out cutting propagation.

1. Preparation of culture medium: Take dry peat soil, perlite and vermiculite according to the ratio of 3:1:1 to form a culture medium, and then put it in an autoclave and sterilize it at 121°C for 40 minutes;

2. Adjustment of the pH value of the culture medium: add an appropriate amount of water to the culture medium to make it moist, adjust the pH of the culture medium to 6.3, then pour the culture medium into the seedling tray for later use, and adjust the pH value of the culture medium with dilute phosphoric acid, Sodium hydroxide solution;

3. Cutting and disinfection of the bulb of the mother plant: take the evergreen cue ball, clean it, remove the outer layer of dry scales, put the bulb up, and cut the cue ball longitudinally from top to bottom into 16 pieces, and ensure that each part has Bulb discs, and then soak the slices of these cut evergreen bulbs in 800-fold diluted carbendazim for 35 minutes for disinfection, and then dip the cut surface in the rooting powder powder, so that the powder is coated on the cut surface of the bulb disc and then taken out as cuttings to be inserted;

4. Planting of bulb slices and cuttings: insert the sterilized cuttings into the culture medium in the seedling tray, the planting row spacing is 4×4 cm, and insert the bulb slices into the culture medium with the direction of the bulb plate, 2/3 of the cuttings Expose the culture substrate, culture in the dark at 22-25°C for 75 days, control the humidity between 70% and 85% during the period, and water thoroughly after each culture substrate is dry;

5. Transplantation of seedlings: When new root buds grow on the bulb slices of the cuttings, transplant them into a culture medium mixed with vinegar grains, humus and perlite at a ratio of 6:1:1, and grow under conventional management conditions. into normal tiger eye evergreen plants.

Example 2

Select a cue ball with a diameter of 26 centimeters and carry out cutting propagation.

1. Preparation of culture medium: Take dry peat soil, perlite and vermiculite according to 3:1:1 and mix into culture medium, then put it in an autoclave and sterilize at 121°C for 35 minutes;

2. Adjustment of the pH value of the culture medium: add an appropriate amount of water to the culture medium to make it moist, adjust the pH of the culture medium to 6.0, then pour the culture medium into the seedling tray for later use, and adjust the pH value of the culture medium with dilute phosphoric acid, Sodium hydroxide solution;

3. Cutting and disinfection of the bulb of the mother plant: take the evergreen cue ball, clean it, remove the outer layer of dry scales, put the bulb up, and cut the cue ball longitudinally from top to bottom into 20 pieces to ensure that each part has a bulb plate, and then soak these cut evergreen bulb slices in 500-fold diluted carbendazim for 40 minutes for disinfection, and then dip the cut surface in the rooting powder powder, so that the powder is coated on the cut surface of the bulb plate , and then taken out as cuttings to be inserted;

4. Planting of bulb slices and cuttings: insert the sterilized cuttings into the culture medium in the seedling tray, the planting row spacing is 4×4 cm, and insert the bulb slices into the culture medium with the direction of the bulb plate, 2/3 of the cuttings Expose the culture substrate, place it in a dark condition at 22-25°C for 85 days, and control the humidity between 70% and 85% during the period, and water it thoroughly after each culture substrate is dry;

5. Transplantation of seedlings: When new root buds grow on the bulb slices of the cuttings, transplant them into a culture medium mixed with vinegar grains, humus and perlite at a ratio of 6:1:1, and grow under conventional management conditions. into normal tiger eye evergreen plants.

Claims (1)

1. Ornithogalum caudatum's bulb cutting cuttage breeding method, is characterized in that comprising the following steps:

1) preparation of culture matrix: get dry turfy soil, perlite and vermiculite were mixed into culture matrix according to 3: 1: 1, is then placed in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 30～50 minutes;

2) the pH value of culture matrix regulates: in culture matrix, add amount of water to make it moistening, regulate culture matrix pH between 6.0～6.5, then culture matrix is poured in seedling-cultivation plate for subsequent usely, while regulating culture matrix pH value, can be used phosphoric acid,diluted, sodium hydroxide solution;

3) cutting of maternal plant bulb and sterilization: get Ornithogalum caudatum's mother bulb that Zhou Jing is greater than 20cm, clean up, remove outer withered scale, plateau upwards, rip cutting mother bulb is divided into 16～20 from top to bottom, and guarantee that every part is all with plateau, then Ornithogalum caudatum's bulb section of these well cuttings is soaked to sterilization in 30～40 minutes in the carbendazim of 500～1000 times of dilutions, and then tangent plane part is dipped in root-inducing powder pulvis, make medicinal powder coated on the tangent plane of plateau, then take out as cuttings to be inserted;

4) plantation of bulb section cuttings: the cuttings of sterilization is inserted in the culture matrix in seedling-cultivation plate, 4 × 4 centimetres of plantation line spaces, under the direction that bulb is cut into slices with plateau, insert culture matrix, 2/3 of cuttings is exposed culture matrix, be placed under the dark condition of 22～25 DEG C and cultivate 70～90 days, controlled humidity, between 70%～85%, and waters permeable after each culture matrix is dry during this time;

5) transplanting of seedling: when growing after new radical bud in the bulb section of cuttings, transplant to vinegar grain, humus soil and perlite according in the culture matrix being mixed at 6: 1: 1, grow up to normal Ornithogalum caudatum plant under Routine Management condition.