CN103931392A - Cutting propagation method for ornithogalum caudatum ait bulb - Google Patents
Cutting propagation method for ornithogalum caudatum ait bulb Download PDFInfo
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- CN103931392A CN103931392A CN201410143781.XA CN201410143781A CN103931392A CN 103931392 A CN103931392 A CN 103931392A CN 201410143781 A CN201410143781 A CN 201410143781A CN 103931392 A CN103931392 A CN 103931392A
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Abstract
The invention belongs to the fields of plant cultivation techniques, landscape plants and ornamental horticulture and relates to a cutting propagation method for an ornithogalum caudatum ait bulb. In the technical scheme, turfy soil, perlite and vermiculite are mixed according to a certain proportion to form a cultivation medium for cutting propagation of the ornithogalum caudatum ait bulb, and differentiation growing and the reproductive rate of the cut ornithogalum caudatum ait bulb sections are improved by means of the technical conditions and methods of adjusting the suitable PH value of the medium, providing suitable cultivating temperature and humidity and the like. According to the cutting propagation method for the ornithogalum caudatum ait bulb, the cultivation medium for the cutting propagation is optimized, so that the cut ornithogalum caudatum ait bulb sections are in an excellent growing environment, differentiation growing and the reproductive rate of the cut ornithogalum caudatum ait bulb sections can be effectively improved, and the simple and practical technology for producing ornithogalum caudatum ait seedlings is provided for vast flower production enterprises.
Description
Technical field
The present invention relates to a kind of Ornithogalum caudatum's bulb cutting cuttage breeding method.The invention belongs to plant cultivation technology, Ornamental Plants and Horticulture field.
Background technology
Ornithogalum caudatum, another name Urginea maritima, bird breast flower.Be a kind of herbaceos perennial of Liliaceae Rohdea, originate in south, Africa.Bulb ovoid, green, diameter can reach 10 centimetres.Leaf 5-6 piece, band shape or strip lanceolar, long 30-60 centimetre, wide 2.5-5 centimetre.As far back as the seventies, Ornithogalum caudatum introduces China with a kind of ornamental plants, and in Hainan, Xi'an, Changbai Mountain examination plants.After numerous scholar's experimental studies, find, from Ornithogalum caudatum, be separated to OSW-1 (being called for short OSW-1).In physiologically active screening experiment, find: " OSW-1 " Anticancer Activity in vitro is than strong ten times even hundred times of the cancer therapy drugs such as the taxol of clinical use, adriamycin, camptothecine.And normal cell is not had to toxicity.From Ornithogalum caudatum, isolated saponin OSW-1 has obvious active anticancer to lung cancer, breast cancer, is common cancer therapy drug camptothecine, adriamycin, taxol active anticancer 10～100 times.This surprising discovery will mean that Ornithogalum caudatum likely replaces the medicines such as taxol, adriamycin, camptothecine, and economic worth is very huge, also have very high ornamental value as a kind of ornamental flower simultaneously.At present Ornithogalum caudatum's propagation method is mainly taking bulb separation and seed propagation as main, although more easily survive a ripe kind ball, annual bulb separation is less than 10, and reproductive efficiency is very low, and seed propagation emergence rate is not high, breeding cycle is long, about about 3 years, has greatly affected production efficiency.Cuttage breeding method has simple to operate, and survival rate is high, and good technique effect is easily accepted and produce to the advantage that production cost is low by market, and the method is applicable to applying very much.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of Ornithogalum caudatum's bulb cutting cuttage breeding method is provided, simple and practical, with low cost, be convenient to extensive artificial cultivation, fast and effeciently a large amount of production high-quality Ornithogalum caudatum seedling.
For realizing such object, in technical scheme of the present invention, adopt a certain proportion of turfy soil, perlite and vermiculite mix, the culture matrix of composition Ornithogalum caudatum bulb section cottage propagation, by regulating suitable matrix acid-base value, the suitable technical conditions such as cultivation temperature and humidity means being provided, improve Ornithogalum caudatum's bulb section differential growth and the reproduction rate of cuttage.
Object of the present invention is achieved by the following technical programs.
1, the preparation of culture matrix: get dry turfy soil, perlite and vermiculite were mixed into culture matrix according to 3: 1: 1, is then placed in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 30～50 minutes;
2, the pH value of culture matrix regulates: in culture matrix, add amount of water to make it moistening, regulate culture matrix pH between 6.0～6.5, then culture matrix is poured in seedling-cultivation plate for subsequent usely, while regulating culture matrix pH value, can be used phosphoric acid,diluted, sodium hydroxide solution;
3, the cutting of maternal plant bulb and sterilization: get Ornithogalum caudatum's mother bulb that Zhou Jing is greater than 20cm, clean up, remove outer withered scale, plateau upwards, rip cutting mother bulb is divided into 16～20 from top to bottom, and guarantee that every part is all with plateau, then Ornithogalum caudatum's bulb section of these well cuttings is soaked to sterilization in 30～40 minutes in the carbendazim of 500～1000 times of dilutions, and then tangent plane part is dipped in root-inducing powder pulvis, make medicinal powder coated on the tangent plane of plateau, then take out as cuttings to be inserted;
4, the plantation of bulb section cuttings: the cuttings of sterilization is inserted in the culture matrix in seedling-cultivation plate, 4 × 4 centimetres of plantation line spaces, under the direction that bulb is cut into slices with plateau, insert culture matrix, 2/3 of cuttings is exposed culture matrix, be placed under the dark condition of 22～25 DEG C and cultivate 70～90 days, controlled humidity, between 70%～85%, and waters permeable after each culture matrix is dry during this time;
5, the transplanting of seedling: when growing after new radical bud in the bulb section of cuttings, transplant to vinegar grain, humus soil and perlite according in the culture matrix being mixed at 6: 1: 1, grow up to normal Ornithogalum caudatum plant under Routine Management condition.
A kind of Ornithogalum caudatum's bulb cutting cuttage breeding method of the present invention is easy and simple to handle, by optimizing the culture matrix of cottage propagation, Ornithogalum caudatum's bulb of cutting is cut into slices in good growing environment, can effectively improve differential growth and the reproduction rate of Ornithogalum caudatum's bulb section of cuttage.
Can be the fast and effeciently a large amount of production high-quality Ornithogalum caudatum seedlings of the inventive method, for vast production of flowers and plants enterprise provides a kind of technology of simple and practical production Ornithogalum caudatum seedling.
Embodiment
The embodiment passing through is below further described technical scheme of the present invention.Following examples do not form limitation of the invention.
Embodiment 1
Selection Zhou Jing is that Ornithogalum caudatum's mother bulb of 22 centimetres carries out cottage propagation.
1, the preparation of culture matrix: get dry turfy soil, perlite and vermiculite were mixed into culture matrix according to 3: 1: 1, is then placed in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 40 minutes;
2, the pH value of culture matrix regulates: in culture matrix, add amount of water to make it moistening, regulate culture matrix pH between 6.3, then culture matrix is poured in seedling-cultivation plate for subsequent usely, while regulating culture matrix pH value, can use phosphoric acid,diluted, sodium hydroxide solution;
3, the cutting of maternal plant bulb and sterilization: get Ornithogalum caudatum's mother bulb, clean up, remove outer withered scale, plateau upwards, rip cutting mother bulb is divided into 16 from top to bottom, and guarantees that every part is all with plateau, then Ornithogalum caudatum's bulb section of these well cuttings is soaked to sterilization in 35 minutes in the carbendazim of 800 times of dilutions, and then tangent plane part is dipped in root-inducing powder pulvis, make medicinal powder coated on the tangent plane of plateau, then take out as cuttings to be inserted;
4, the plantation of bulb section cuttings: the cuttings of sterilization is inserted in the culture matrix in seedling-cultivation plate, 4 × 4 centimetres of plantation line spaces, under the direction that bulb is cut into slices with plateau, insert culture matrix, 2/3 of cuttings is exposed culture matrix, be placed under the dark condition of 22～25 DEG C and cultivate 75 days, controlled humidity, between 70%～85%, and waters permeable after each culture matrix is dry during this time;
5, the transplanting of seedling: when growing after new radical bud in the bulb section of cuttings, transplant to vinegar grain, humus soil and perlite according in the culture matrix being mixed at 6: 1: 1, grow up to normal Ornithogalum caudatum plant under Routine Management condition.
Embodiment 2
Selection Zhou Jing is that Ornithogalum caudatum's mother bulb of 26 centimetres carries out cottage propagation.
1, the preparation of culture matrix: get dry turfy soil, perlite and vermiculite were mixed into culture matrix according to 3: 1: 1, is then placed in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 35 minutes;
2, the pH value of culture matrix regulates: in culture matrix, add amount of water to make it moistening, regulate culture matrix pH between 6.0, then culture matrix is poured in seedling-cultivation plate for subsequent usely, while regulating culture matrix pH value, can use phosphoric acid,diluted, sodium hydroxide solution;
3, the cutting of maternal plant bulb and sterilization: get Ornithogalum caudatum's mother bulb, clean up, remove outer withered scale, plateau upwards, rip cutting mother bulb is divided into 20 from top to bottom, guarantees that every part is all with plateau, then Ornithogalum caudatum's bulb section of these well cuttings is soaked to sterilization in 40 minutes in the carbendazim of 500 times of dilutions, and then tangent plane part is dipped in root-inducing powder pulvis, make medicinal powder coated on the tangent plane of plateau, then take out as cuttings to be inserted;
4, the plantation of bulb section cuttings: the cuttings of sterilization is inserted in the culture matrix in seedling-cultivation plate, 4 × 4 centimetres of plantation line spaces, under the direction that bulb is cut into slices with plateau, insert culture matrix, 2/3 of cuttings is exposed culture matrix, be placed under the dark condition of 22～25 DEG C and cultivate 85 days, controlled humidity, between 70%～85%, and waters permeable after each culture matrix is dry during this time;
5, the transplanting of seedling: when growing after new radical bud in the bulb section of cuttings, transplant to vinegar grain, humus soil and perlite according in the culture matrix being mixed at 6: 1: 1, grow up to normal Ornithogalum caudatum plant under Routine Management condition.
Claims (1)
1. Ornithogalum caudatum's bulb cutting cuttage breeding method, is characterized in that comprising the following steps:
1) preparation of culture matrix: get dry turfy soil, perlite and vermiculite were mixed into culture matrix according to 3: 1: 1, is then placed in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 30～50 minutes;
2) the pH value of culture matrix regulates: in culture matrix, add amount of water to make it moistening, regulate culture matrix pH between 6.0～6.5, then culture matrix is poured in seedling-cultivation plate for subsequent usely, while regulating culture matrix pH value, can be used phosphoric acid,diluted, sodium hydroxide solution;
3) cutting of maternal plant bulb and sterilization: get Ornithogalum caudatum's mother bulb that Zhou Jing is greater than 20cm, clean up, remove outer withered scale, plateau upwards, rip cutting mother bulb is divided into 16～20 from top to bottom, and guarantee that every part is all with plateau, then Ornithogalum caudatum's bulb section of these well cuttings is soaked to sterilization in 30～40 minutes in the carbendazim of 500～1000 times of dilutions, and then tangent plane part is dipped in root-inducing powder pulvis, make medicinal powder coated on the tangent plane of plateau, then take out as cuttings to be inserted;
4) plantation of bulb section cuttings: the cuttings of sterilization is inserted in the culture matrix in seedling-cultivation plate, 4 × 4 centimetres of plantation line spaces, under the direction that bulb is cut into slices with plateau, insert culture matrix, 2/3 of cuttings is exposed culture matrix, be placed under the dark condition of 22～25 DEG C and cultivate 70～90 days, controlled humidity, between 70%～85%, and waters permeable after each culture matrix is dry during this time;
5) transplanting of seedling: when growing after new radical bud in the bulb section of cuttings, transplant to vinegar grain, humus soil and perlite according in the culture matrix being mixed at 6: 1: 1, grow up to normal Ornithogalum caudatum plant under Routine Management condition.
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| CN201410143781.XA CN103931392A (en) | 2014-04-11 | 2014-04-11 | Cutting propagation method for ornithogalum caudatum ait bulb |
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| CN201410143781.XA CN103931392A (en) | 2014-04-11 | 2014-04-11 | Cutting propagation method for ornithogalum caudatum ait bulb |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561690A (en) * | 2016-10-25 | 2017-04-19 | 徐州得铸生物科技有限公司 | Insecticide composite containing rhodexinA |
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129124A (en) * | 2007-09-27 | 2008-02-27 | 上海交通大学 | Hippeastrum bulb cutting cuttage breeding method |
| CN102090252A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage propagation method of allium giganteum |
-
2014
- 2014-04-11 CN CN201410143781.XA patent/CN103931392A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129124A (en) * | 2007-09-27 | 2008-02-27 | 上海交通大学 | Hippeastrum bulb cutting cuttage breeding method |
| CN102090252A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage propagation method of allium giganteum |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561690A (en) * | 2016-10-25 | 2017-04-19 | 徐州得铸生物科技有限公司 | Insecticide composite containing rhodexinA |
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
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