CN103882066A - Preparation method of novel calcium supplement-calcium lactate gluconate - Google Patents
Preparation method of novel calcium supplement-calcium lactate gluconate Download PDFInfo
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- CN103882066A CN103882066A CN201210557869.7A CN201210557869A CN103882066A CN 103882066 A CN103882066 A CN 103882066A CN 201210557869 A CN201210557869 A CN 201210557869A CN 103882066 A CN103882066 A CN 103882066A
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- lactate gluconate
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Abstract
The invention relates to a preparationg method of calcium lactate gluconate. Calcium lactate gluconate is prepared through double enzyme catalysis with glucose, calcium carbonate and lactic acid as raw materials, and catalase and glucose oxidase as enzyme catalysts. The raw materials concretely comprise 100wt% of glucose, 50-60wt% of calcium carbonate and 30-100wt% of lactic acid; and the addition amount of catalase is 0.3-1.2% of the amount of a glucose dry basis, and the addition amount of glucose oxidase is 0.5-1.5% of the amount of the glucose dry basis. The calcium lactate gluconate product prepared by adopting the method has the advantages of high purity and high solubility, can be used as a new calcium supplements, and has significant advantages.
Description
Technical field
the invention belongs to the technical field of production calcium lactate gluconate, relate in particular to the method for producing calcium lactate gluconate by double-enzyme method.
Background technology
Calcium is one of element of wanting of body weight for humans, and in human body, 99% calcium is deposited in bone, and all the other are 1% in blood circulation.Calcium has important biochemical functions, its Main Function be form bone, maintain nerve and muscle normal excitement, reduce capillary vessel permeability, participate in coagulation process calcium and be constantly consumed, must be supplemented by diet etc.And calcium deficiency is the most serious in the dietary nutrition of China, calcium intake on average only reaches feed rate 50% every day, lacks phenomenon more obvious in the crowds such as pregnant woman, children, old man.Therefore developing the calcium-nutrition intensifying agent that a kind of solubleness is high, specific absorption is good is current problem demanding prompt solution.
Calcium lactate gluconate (CLG) is that a kind of newer calcium supplementing product is applied in F&B.Up to the present, this has been a kind of calcium source medicine.The remarkable spy of this product innovation is high resolution and soft mouthfeel, can improve the quality of food or medicine, so be range of application calcium product-derived more widely.The solubleness of calcium lactate gluconate is than single calcium preparation, as large in the solubleness of calcium lactate, calisanin and calcium lactate and calisanin mixing, and technique is simpler, has good development prospect.
At present, the domestic methods involving report that also there is no desirable production calcium lactate gluconate.
Summary of the invention
The object of the invention is to, a kind of novel method of producing newly calcium source-calcium lactate gluconate is provided, by method provided by the invention, the calcium lactate gluconate product of producing, solubleness is large, and calcium contents is high, good absorbing effect.Meanwhile, this law is also the production method of a kind of efficient, low consumption, high-quality, energy-conservation and environmental protection.The all raw materials of the method all meet the requirement of food sanitation, and product safety is reliable.The product calcium lactate gluconate of producing by the method meets relevant external product standard completely.Therefore, the present invention develops calcium lactate gluconate voluntarily by the method for science, has filled up industry blank.
Technical scheme of the present invention is a kind ofly to get the preparation method of calcium lactate gluconate with catalase and glucose oxidase double-enzyme catalysis legal system.
This preparation method is taking glucose, calcium carbonate and lactic acid as raw material, taking catalase and glucose oxidase as double-enzyme catalysis agent, and preparation calcium lactate gluconate.
In aforesaid method, selected raw material consumption part by weight is: glucose (butt) 100%, calcium carbonate 50-60%, lactic acid (folding 100%) 30-100%; Catalase add-on is the 0.3-1.2 % of glucose butt, glucose oxidase add-on is the 0.5-1.5 % of glucose butt, wherein, the zymetology classification number of glucose oxidase is E.C.1.1.3.4, enzyme activity is 100000U/g, catalatic zymetology classification number is EC 1.11.1.6, and enzyme activity is 50000 U/g.
The concrete operation step of the preparation method of calcium lactate gluconate of the present invention is as follows:
(1) solution that, preparation dry substance concentration is 10-20%.
(2), disposablely add excessive calcium carbonate, the add-on of calcium carbonate is the 50-60% of glucose butt quality, then add a certain amount of lactic acid, the add-on of lactic acid is the 30-100% of glucose quality, add water, be uniformly mixed, preparation becomes the reaction solution containing raw material (butt) 0.35-0.50kg/L.
(3), first to catalase in reaction solution, add-on is the 0.3-1.2 % of glucose butt, then adds glucose oxidase, add-on is the 0.5-1.5 % of glucose butt;
Preferably adding after catalase 1-10 minute, then adding glucose oxidase;
Air flow (VVm) is 0.6-1.2, and retort pressure remains on 0.1-0.2MPa, and stirring velocity is 450-500r/min, and enzyme reaction temperature is 32-39 DEG C.
(4), in the time that in assaying reaction liquid, concentration of reduced sugar is 0.1%, finish reaction.By reaction solution heat up go out enzyme, to decolour, be concentrated to dry substance concentration be 20-40%, carries out decrease temperature crystalline by cooling process, then through separating, the dry finished product calcium lactate gluconate that obtains.
The invention has the beneficial effects as follows: this law technique is simple, the calcium lactate gluconate product purity of producing by this law is high, and solubleness is large, compare with other calcium preparation, solubleness has a clear superiority in, and biological value also has a clear superiority in than other calsium supplement, and market potential is huge.
Embodiment
embodiment 1:
| Raw material | Usage ratio | Actual addition |
| Glucose | 100% | 1112g |
| Calcium carbonate | 51% | 566.5g |
| Lactic acid | 100% | 1112g |
| Glucose oxidase | 1.2% | 4.62ml |
| Catalase | 0.8% | 5.94ml |
Note: above show Raw usage ratio % and refer to taking raw materials of glucose and roll over 100% butt as benchmark, the consumption % by weight (as follows) of needed other raw materials.
By the raw material in upper table, preparation reaction solution 6000 ml, temperature of reaction is 35 DEG C.Pass into sterile air, control air flow is 1:1v/v/min, and retort pressure remains on 0.2MPa, and be 25 hours reaction time, when concentration of reduced sugar in reaction solution be down to≤0.1% time, finish reaction.Reaction solution being warming up to 95 DEG C of enzymes that go out, adding gac at 60 DEG C, to decolour 1 hour by 0.1% (W:V), filter to get filtrate, is under 0.095MPa condition in vacuum tightness, and 60 DEG C are evaporated to dry substance concentration is 40%.Crystallization under room temperature, then separates and is dried, and obtains calcium lactate gluconate finished product 1489g.The yield of calcium lactate gluconate based on glucose is 134%.
embodiment 2:
| Raw material | Usage ratio | Actual addition |
| Glucose | 100% | 1112g |
| Calcium carbonate | 51% | 566.5g |
| Lactic acid | 50% | 556g |
| Glucose oxidase | 1.2% | 4.62ml |
| Catalase | 0.8% | 5.94ml |
By the raw material in upper table, preparation reaction solution 6000 ml, temperature of reaction is 35 DEG C.Pass into sterile air, control air flow is 1:1v/v/min, and retort pressure remains on 0.2MPa, and be 25 hours reaction time, when concentration of reduced sugar in reaction solution be down to≤0.1% time, finish reaction.Reaction solution being heated up as for 95 DEG C of enzymes that go out, add gac at 60 DEG C, to decolour 1 hour by 0.1% (W:V), filter to get filtrate, is under 0.095MPa condition in vacuum tightness, and 60 DEG C are evaporated to dry substance concentration is 40%.Crystallization under room temperature, then separates and is dried, and obtains calcium lactate gluconate finished product 1317g.The yield of calcium lactate gluconate based on glucose is 119%.
embodiment 3:
| Raw material | Ratio | Actual addition |
| Glucose | 100% | 1112g |
| Calcium carbonate | 51% | 566.5g |
| Lactic acid | 33% | 367g |
| Glucose oxidase | 1.2% | 4.62ml |
| Catalase | 0.8% | 5.94ml |
By the raw material in upper table, preparation reaction solution 6000 ml, temperature of reaction is 35 DEG C.Pass into sterile air, control air flow is 1:1v/v/min, and retort pressure remains on 0.2MPa, and be 25 hours reaction time, when reaction is down to≤is finished 0.1% time to concentration of reduced sugar in reaction solution.Reaction solution being heated up as for 95 DEG C of enzymes that go out, add gac at 60 DEG C, to decolour 1 hour by 0.1% (W:V), filter to get filtrate, is under 0.095MPa condition in vacuum tightness, and 60 DEG C are evaporated to dry substance concentration is 40%.Crystallization under room temperature, then separates and is dried, and obtains calcium lactate gluconate finished product 1279g.The yield of calcium lactate gluconate based on glucose is 115%.
Claims (8)
1. a preparation method for novel calsium supplement-calcium lactate gluconate, it is characterized in that taking glucose, calcium carbonate and lactic acid as raw material, and taking catalase and glucose oxidase as enzyme catalyst, double-enzyme catalysis is produced the method for calcium lactate gluconate.
2. the method for producing calcium lactate gluconate as claimed in claim 1, is characterized in that described raw material consumption part by weight is: glucose 100%, calcium carbonate 50-60%, lactic acid 30-100%; Catalase add-on is the 0.3-1.2% of glucose butt, and glucose oxidase add-on is the 0.5-1.5 % of glucose butt.
3. the method for producing calcium lactate gluconate as claimed in claim 1 or 2, the zymetology classification number that it is characterized in that described glucose oxidase is E.C.1.1.3.4, enzyme activity is 100000U/g, and catalatic zymetology classification number is EC 1.11.1.6, and enzyme activity is 50000 U/g.
4. the method for producing calcium lactate gluconate as claimed in claim 1, is characterized in that described raw material consumption part by weight is: glucose 100%, calcium carbonate 51%, lactic acid 100%; Catalase add-on is 0.8 % of glucose butt, and glucose oxidase add-on is 1.2 % of glucose butt.
5. the method for producing calcium lactate gluconate as claimed in claim 1, is characterized in that described raw material consumption part by weight is: glucose 100%, calcium carbonate 51%, lactic acid 50%; Catalase add-on is 0.8 % of glucose butt, and glucose oxidase add-on is 1.2 % of glucose butt.
6. the method for producing calcium lactate gluconate as claimed in claim 1, is characterized in that described raw material consumption part by weight is: glucose 100%, calcium carbonate 51%, lactic acid 33%; Catalase add-on is 0.8 % of glucose butt, and glucose oxidase add-on is 1.2 % of glucose butt.
7. the method for producing calcium lactate gluconate as claimed in claim 1, is characterized in that comprising the following steps:
(1) glucose solution that, compound concentration is 10-20%;
(2), disposablely add excessive calcium carbonate, the 50-60% that the add-on of calcium carbonate is glucose quality, then adds a certain amount of lactic acid, the 30-100% that the add-on of lactic acid is glucose quality, adds water, and through being uniformly mixed, is reaction solution;
(3), first to catalase in reaction solution, add-on is the 0.3-1.2 % of glucose butt, then adds glucose oxidase, add-on is the 0.5-1.5 % of glucose butt;
(4), reaction conditions is: air flow (VVm) is 0.6-1.2, and retort pressure is 0.1-0.2MPa, and stirring velocity is 450-500r/min, and temperature of reaction is 32-39 DEG C;
(5), when concentration of reduced sugar in assaying reaction liquid is to≤0.1% time, finish reaction; By reaction solution heat up go out enzyme, to decolour, be concentrated to dry substance concentration be 20-40%, carries out crystallization by cooling process, then through separating, the dry finished product calcium lactate gluconate that obtains.
8. the method for producing calcium lactate gluconate as claimed in claim 7, is characterized in that, in described step (3), adding after catalase 1-10 minute, then adding glucose oxidase.
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| CN201210557869.7A CN103882066A (en) | 2012-12-20 | 2012-12-20 | Preparation method of novel calcium supplement-calcium lactate gluconate |
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| CN201210557869.7A CN103882066A (en) | 2012-12-20 | 2012-12-20 | Preparation method of novel calcium supplement-calcium lactate gluconate |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1719986A (en) * | 2002-11-22 | 2006-01-11 | 萨拉·A.·卡拉比尼 | Calcium fortified acidic beverages |
| CN101333543A (en) * | 2007-04-03 | 2008-12-31 | 浙江天益食品添加剂有限公司 | Method for manufacturing calcium gluconate by enzyme method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1719986A (en) * | 2002-11-22 | 2006-01-11 | 萨拉·A.·卡拉比尼 | Calcium fortified acidic beverages |
| CN101333543A (en) * | 2007-04-03 | 2008-12-31 | 浙江天益食品添加剂有限公司 | Method for manufacturing calcium gluconate by enzyme method |
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Application publication date: 20140625 |