CN103875447B - The two-way cultivation box of one Plants and AM mycosymbiosis - Google Patents
The two-way cultivation box of one Plants and AM mycosymbiosis Download PDFInfo
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- CN103875447B CN103875447B CN201410055399.3A CN201410055399A CN103875447B CN 103875447 B CN103875447 B CN 103875447B CN 201410055399 A CN201410055399 A CN 201410055399A CN 103875447 B CN103875447 B CN 103875447B
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Abstract
The two-way cultivation box of one Plants and AM mycosymbiosis, fill nutrient solution in liquid storage tank, it connects support plate, lay waterleaf paper and mycelia filter membrane on support plate, waterleaf paper two ends are immersed in nutrient solution, phytotron is established on support plate top, and both sides arrange mycorhiza room respectively and change liquid mouth, the indoor placement culture matrix of plant growth and communicate with bottom mycorhiza room, its top seals with breathable sealing film respectively.The present invention can make nutrient solution automatically enter mycorhiza room and phytotron, root system of plant is grown along mycelia filter membrane, enter behind mycorhiza room until root system and AM fungal spore is inoculated in root system surface, can realize observing AM hypha,hyphae characteristic and the forming process with host plant Root Symbiont structure under transparent condition directly perceived, research photosynthesis of plant and AM fungal physiology biochemical characteristic.Present invention achieves the dual cultivation to AM fungi and whole strain plant, for studying AM fungi and plant relationship and providing an effective way to the effect of plant.
Description
Technical field
The present invention relates to the cultivation box of a Plants, the two-way cultivation box of specifically plant and AM mycosymbiosis, belongs to fungi basic research field.
Background technology
By the restriction of arbuscular mycorrhiza (ArbuscularMycorrhiza, AM) mycosymbiosis characteristic, be difficult to for a long time study it under transparent condition intuitively, cause the research for aspects such as AM hypha,hyphae structure and physio-biochemical characteristics thereof seriously delayed.In recent years, utilize agrobacterium rhizogenes (
agrobacteriumrhizogenes) inducing plant root system generation hair shape root, and successfully establish the dual culture technique of excised root (Wang Jingjing etc., 2010 on this basis; Kastell
etal., 2013).This technology can realize morphological development and the physio-biochemical characteristics thereof of observing AM fungi at culture dish situ, is a kind of effective ways of generally acknowledging research AM hypha,hyphae physio-biochemical characteristics at present in the world.But, the method still can not cultivate whole strain plant by the restriction of culture dish space and nutritional condition, namely can not study AM fungi when aboveground vegetation part and photosynthesis participate in, sucrose can only be added in the medium to replace photosynthesis, thus limit the application of this technology in some researchs, as research carbohydrate and the mutual transmission etc. of mineral matter between fungi and aboveground vegetation part.Thus, invent a kind of plant growth that had both been applicable to and be also applicable to the culture vessel of AM conk and just seem particularly important, still do not have a kind of desirable culture vessel available at present.
Summary of the invention
For realizing the dual cultivation to whole strain plant and AM fungi, the invention discloses the two-way cultivation box of a Plants and AM mycosymbiosis.
The present invention is achieved by the following technical solutions:
Nutrient solution is filled in liquid storage tank, support plate is connected between the front and rear wall of liquid storage tank top, lay waterleaf paper on support plate, waterleaf paper is placed mycelia filter membrane, the prolongation of waterleaf paper two ends is dipped vertically in nutrient solution, support plate middle upper portion is provided with phytotron, the U-shaped dividing plate of phytotron both sides arranges mycorhiza room outward respectively, two mycorhiza outsides establish displacement liquid mouth respectively, the indoor placement culture matrix of plant growth, phytotron communicates with bottom two mycorhiza rooms, its top seals with breathable sealing film respectively.
The present invention utilizes waterleaf paper to make nutrient solution automatically enter mycorhiza room and phytotron, root system of plant in phytotron grows into mycorhiza room along mycelia filter membrane, enter behind mycorhiza room until root system and the AM fungal spore of surface sterilization is inoculated in root system surface, regularly under Stereo microscope, observe AM fungi, can realize observing AM hypha,hyphae characteristic and the forming process with host plant Root Symbiont structure thereof under transparent condition intuitively, research has the AM fungal physiology biochemical characteristic in photosynthesis of plant and acrial part participation situation.The present invention is reasonable in design, and structure is simple, with low cost, easy to use, achieves the dual cultivation to AM fungi and whole strain plant simultaneously, for furtheing investigate AM fungi and plant relationship and providing an effective way to the effect of plant.
Accompanying drawing explanation
Accompanying drawing 1 is the main TV structure generalized section of the embodiment of the present invention.
Accompanying drawing 2 is vertical views of embodiment of the present invention accompanying drawing 1 half overlay film.
Accompanying drawing 3 are embodiment of the present invention accompanying drawings 1 along A-A to generalized section.
Description of reference numerals is as follows:
Phytotron 1, U-shaped dividing plate 2, culture matrix 3, mycelia filter membrane 4, mycorhiza room 5, waterleaf paper 6, liquid storage tank 7, nutrient solution 8, support plate 9, breathable sealing film 10, changes liquid mouth 11.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail:
Be fill nutrient solution (8) in 36 × 10 × 10 ~ 20cm liquid storage tank (7) in specification, support plate (9) is connected between the front and rear wall of liquid storage tank (7) top, the upper lay waterleaf paper (6) of support plate (9), waterleaf paper (6) is upper places mycelia filter membrane (4), the prolongation of waterleaf paper (6) two ends is dipped vertically in nutrient solution (8), support plate (9) middle upper portion is provided with the phytotron (1) of 10 × 10 × 20 ~ 30cm, the U-shaped dividing plate (2) of phytotron (1) both sides arranges the mycorhiza room (5) of 10 × 10 × 3 ~ 5cm outward respectively, displacement liquid mouth (11) is established in two mycorhiza room (5) outsides respectively, quartz sand is placed as culture matrix (3) in phytotron (1), phytotron (1) communicates with two mycorhiza room (5) bottoms, its top uses breathable sealing film (10) to seal respectively.
Passing through in liquid storage tank, increase nutrient solution when the present invention uses makes the distance of nutrition liquid level and support plate remain at 3 ~ 10cm, high pressure damp and hot (121 DEG C) sterilizing 20min, after taking out cooling, the plant seed of surface sterilization is placed in culture matrix, then (temperature is 20 ~ 25 DEG C, light application time 12 ~ 14hd this cultivation box to be put into climatic cabinate
-1) middle cultivation; According to vegetation growth state during cultivation, every 3 ~ 7d aseptically opens sealed membrane, being blotted by the nutrient solution in liquid storage tank, then rejoining new nutrient solution, to ensure that plant well grows by changing liquid mouth liquid-transfering gun; When root system of plant grows into mycorhiza room along mycelia filter membrane, the AM fungal spore of surface sterilization is inoculated in root system surface; Because mycorhiza room is without culture matrix and sealed membrane is transparent, therefore can directly observe under the microscope AM fungal spore sprouting, infect and the forming process etc. of spore, thus easily realize observational study AM fungi under transparent condition intuitively, for further investigation AM fungi provides a desirable culture vessel.
Claims (3)
1. the two-way cultivation box of a Plants and AM mycosymbiosis, it is characterized in that being achieved through the following technical solutions: in liquid storage tank (7), fill nutrient solution (8), support plate (9) is connected between the front and rear wall of liquid storage tank (7) top, the upper lay waterleaf paper (6) of support plate (9), waterleaf paper (6) is upper places mycelia filter membrane (4), the prolongation of waterleaf paper (6) two ends is dipped vertically in nutrient solution (8), support plate (9) middle upper portion is provided with phytotron (1), the U-shaped dividing plate (2) of phytotron (1) both sides arranges mycorhiza room (5) outward respectively, displacement liquid mouth (11) is established in two mycorhiza room (5) outsides respectively, culture matrix (3) is placed in phytotron (1), phytotron (1) communicates with two mycorhiza room (5) bottoms, its top uses breathable sealing film (10) to seal respectively.
2. the two-way cultivation box of a Plants according to claim 1 and AM mycosymbiosis, it is characterized in that liquid storage tank (7) specification is 36 × 10 × 10 ~ 20cm, phytotron (1) specification is 10 × 10 × 20 ~ 30cm, and mycorhiza room (5) specification is 10 × 10 × 3 ~ 5cm.
3. the two-way cultivation box of a Plants according to claim 1 and AM mycosymbiosis, is characterized in that culture matrix (3) is quartz sand.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410055399.3A CN103875447B (en) | 2014-02-19 | 2014-02-19 | The two-way cultivation box of one Plants and AM mycosymbiosis |
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| CN201410055399.3A CN103875447B (en) | 2014-02-19 | 2014-02-19 | The two-way cultivation box of one Plants and AM mycosymbiosis |
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| Publication Number | Publication Date |
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| CN103875447A CN103875447A (en) | 2014-06-25 |
| CN103875447B true CN103875447B (en) | 2016-01-20 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186198B (en) * | 2014-07-02 | 2016-06-08 | 贵州大学 | For the Nutrient retranslocation acting device between plant |
| CN104830663A (en) * | 2015-04-13 | 2015-08-12 | 青岛农业大学 | Double room hypha test apparatus and double room hypha test method |
| CN104996183B (en) * | 2015-07-28 | 2018-08-17 | 中国农业大学 | A kind of method and its dedicated unit of influence of the detection microorganism to root system of plant |
| CN106190944B (en) * | 2016-07-07 | 2019-10-01 | 华南农业大学 | A method of layering culture enrichment arbuscular mycorrhizal fungi spore |
| CN106508429A (en) * | 2016-11-10 | 2017-03-22 | 中国矿业大学(北京) | Multiplication method of arbuscular mycorrhiza fungi inoculant in coal mine area |
| CN106576910B (en) * | 2016-12-14 | 2020-04-21 | 山西省农业科学院食用菌研究所 | Culture method of in-vitro sterile host plant-mycorrhizal edible fungus symbiotic seedling |
| CN106834087B (en) * | 2017-03-01 | 2019-02-22 | 厦门大学 | A kind of the AM fungi numerous and functional experiment device of expansion and application method of timing cycle |
| CN110476713B (en) * | 2019-09-26 | 2021-03-16 | 华南农业大学 | Device and method for continuously collecting arbuscular mycorrhizal fungi hyphae |
| CN113207668A (en) * | 2021-05-17 | 2021-08-06 | 南京农业大学 | Full-automatic soilless culture fungus plants co-culture device |
| CN113817798B (en) * | 2021-09-29 | 2023-07-21 | 广西民族大学 | Potted plant detection device and detection method for plant effect of microorganism volatile substances |
| CN115500178B (en) * | 2022-10-25 | 2024-01-09 | 贵州大学 | Bird king tea mycorrhiza seedling raising device and application method thereof |
Citations (5)
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| US4531324A (en) * | 1983-10-07 | 1985-07-30 | Agracetus | Plant tissue culture device |
| CN1354252A (en) * | 2000-11-21 | 2002-06-19 | 李晓林 | Culture of ramaria mycorrhizal fungi by using glass bead as culture medium |
| WO2005048688A1 (en) * | 2003-11-18 | 2005-06-02 | Her Majesty The Queen | Bio dynamic interface system for plant growing |
| CN101608159A (en) * | 2008-06-20 | 2009-12-23 | 钟明基 | A kind of cultivation from method and the device of the inoculation bacterium of mycorrhizal fungi |
| KR101321652B1 (en) * | 2012-07-12 | 2013-10-23 | 이영섭 | Mycorrhizal fungi cultivating apparatus |
-
2014
- 2014-02-19 CN CN201410055399.3A patent/CN103875447B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4531324A (en) * | 1983-10-07 | 1985-07-30 | Agracetus | Plant tissue culture device |
| CN1354252A (en) * | 2000-11-21 | 2002-06-19 | 李晓林 | Culture of ramaria mycorrhizal fungi by using glass bead as culture medium |
| WO2005048688A1 (en) * | 2003-11-18 | 2005-06-02 | Her Majesty The Queen | Bio dynamic interface system for plant growing |
| CN101608159A (en) * | 2008-06-20 | 2009-12-23 | 钟明基 | A kind of cultivation from method and the device of the inoculation bacterium of mycorrhizal fungi |
| KR101321652B1 (en) * | 2012-07-12 | 2013-10-23 | 이영섭 | Mycorrhizal fungi cultivating apparatus |
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