CN103843888A

CN103843888A - Lactobacillus beverage

Info

Publication number: CN103843888A
Application number: CN201210503864.6A
Authority: CN
Inventors: 杨立飞; 鞠慧; 战靖
Original assignee: QINGDAO HUINENGDUO AGRICULTURAL DEVELOPMENT Co Ltd
Current assignee: QINGDAO HUINENGDUO AGRICULTURAL DEVELOPMENT Co Ltd
Priority date: 2012-12-02
Filing date: 2012-12-02
Publication date: 2014-06-11

Abstract

The invention relates to a lactobacillus beverage. The beverage is obtained by mixing disinfected fresh milk with an acclimated lactobacillus fermentation liquid under certain production conditions. The lactobacillus beverage can be drunk by people of all ages, comprising lactose intolerance people, contains no additives, and is purely natural drink.

Description

A kind of sour milk beverage

Technical field

Add a beverage for lactic acid bacteria, belong to drink industry field.

Technical background

A kind of with cow's milk 40-60% in the market, sugar 7-11%, corn flour 4-6%, honey 0.5-1%, phosphate 0.02-0.06%, stabilizing agent 0.1-0.4%, a kind of beverage taking milk as master of water 20-50% composition, it has nutritious, containing multiple unrighted acid and vitamin, containing synthetic dyestuff, essence, the advantage such as taste is pure, particularly can store for a long time, be a kind of cold and hot all suitable drink.But this milk is unsuitable for the people of lactose intolerance, and the present invention does not add any auxiliary material, natural beverage completely.

Summary of the invention

Fresh milk is heated to 65 DEG C--after 72 DEG C of insulation 10min, 25---under 30 MPa homogeneous once, then by Milk During Heating to 90 DEG C sterilizing 20min, be cooled to 37 DEG C for subsequent use; The ratio that is 2: 2: 1 in thalline number by streptococcus thermophilus, lactobacillus bulgaricus and Bifidobacterium is mixed, then with 10 ⁹cfu/100mL ratio is inoculated in the sterilizing degreasing reconstituted milk of mass concentration 12%, and 37 DEG C leave standstill cultivation 12-14h and activate the mixed bacteria that obtains activation; Sterilizing under 20 minutes conditions of 108 DEG C of insulations after pure soya-bean milk and degreasing reconstituted milk are mixed according to the ratio of volume ratio 0: 10,1: 9,2: 8,3: 7,4: 6 and 5: 5 respectively, is designated as nutrient solution 1, nutrient solution 2, nutrient solution 3, nutrient solution 4, nutrient solution 5 and nutrient solution 6 successively; The mixed bacteria of activation is inoculated in nutrient solution 1, inoculum concentration is 3% of nutrient solution 1 volume, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 1 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 1 of 1 time is inoculated in nutrient solution 1 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 1 of 2 times obtains going down to posterity; By going down to posterity, the mixed bacteria 1 of 2 times is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 2 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 2 of 1 time is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 2 of 2 times obtains going down to posterity; By that analogy, until the mixed bacteria 6 of 2 times that obtains going down to posterity; The mixed bacteria 6 of 2 times of going down to posterity is placed in to aseptic centrifuge tube, and at 2-6 DEG C, centrifugation obtains the lactic acid bacteria thalline of domestication, and with the fresh milk of sterilizing or reconstituted milk, concentration is washed down and be adjusted to the lactic acid bacteria thalline of described domestication is 4 × 10 ⁷-2 × 10 ⁸cfu/mL, obtains the streptococcus acidi lactici fermented solution of taming; The streptococcus acidi lactici fermented solution inoculum concentration of getting domestication is the 4-6% of fresh milk volume, after inoculation 40-42 DEG C ferment to pH be 4.5-4.8, then in 4 DEG C refrigeration 12-24 hour.

Embodiment mono-

Fresh milk is heated to 65 DEG C--after 72 DEG C of insulation 10min, under 25 MPa homogeneous once, then by Milk During Heating to 90 DEG C sterilizing 20min, be cooled to 37 DEG C for subsequent use; The ratio that is 2: 2: 1 in thalline number by streptococcus thermophilus, lactobacillus bulgaricus and Bifidobacterium is mixed, then with 10 ⁹cfu/100mL ratio is inoculated in the sterilizing degreasing reconstituted milk of mass concentration 12%, and 37 DEG C leave standstill cultivation 12-14h and activate the mixed bacteria that obtains activation; Sterilizing under 20 minutes conditions of 108 DEG C of insulations after pure soya-bean milk and degreasing reconstituted milk are mixed according to the ratio of volume ratio 0: 10,1: 9,2: 8,3: 7,4: 6 and 5: 5 respectively, is designated as nutrient solution 1, nutrient solution 2, nutrient solution 3, nutrient solution 4, nutrient solution 5 and nutrient solution 6 successively; The mixed bacteria of activation is inoculated in nutrient solution 1, inoculum concentration is 3% of nutrient solution 1 volume, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 1 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 1 of 1 time is inoculated in nutrient solution 1 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 1 of 2 times obtains going down to posterity; By going down to posterity, the mixed bacteria 1 of 2 times is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 2 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 2 of 1 time is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 14 hours, and the mixed bacteria 2 of 2 times obtains going down to posterity; By that analogy, until the mixed bacteria 6 of 2 times that obtains going down to posterity; The mixed bacteria 6 of 2 times of going down to posterity is placed in to aseptic centrifuge tube, and at 2-6 DEG C, centrifugation obtains the lactic acid bacteria thalline of domestication, and with the fresh milk of sterilizing or reconstituted milk, concentration is washed down and be adjusted to the lactic acid bacteria thalline of described domestication is 4 × 10 ⁷-2 × 10 ⁸cfu/mL, obtains the streptococcus acidi lactici fermented solution of taming; The streptococcus acidi lactici fermented solution inoculum concentration of getting domestication is the 4-6% of fresh milk volume, after inoculation 40-42 DEG C ferment to pH be 4.5-4.8, then in 4 DEG C refrigeration 12 hours.

Embodiment bis-

Fresh milk is heated to 65 DEG C--after 72 DEG C of insulation 10min, under 30MPa homogeneous once, then by Milk During Heating to 90 DEG C sterilizing 20min, be cooled to 37 DEG C for subsequent use; The ratio that is 2: 2: 1 in thalline number by streptococcus thermophilus, lactobacillus bulgaricus and Bifidobacterium is mixed, then with 10 ⁹cfu/100mL ratio is inoculated in the sterilizing degreasing reconstituted milk of mass concentration 12%, and 37 DEG C leave standstill cultivation 12-14h and activate the mixed bacteria that obtains activation; Sterilizing under 20 minutes conditions of 108 DEG C of insulations after pure soya-bean milk and degreasing reconstituted milk are mixed according to the ratio of volume ratio 0: 10,1: 9,2: 8,3: 7,4: 6 and 5: 5 respectively, is designated as nutrient solution 1, nutrient solution 2, nutrient solution 3, nutrient solution 4, nutrient solution 5 and nutrient solution 6 successively; The mixed bacteria of activation is inoculated in nutrient solution 1, inoculum concentration is 3% of nutrient solution 1 volume, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 1 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 1 of 1 time is inoculated in nutrient solution 1 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 1 of 2 times obtains going down to posterity; By going down to posterity, the mixed bacteria 1 of 2 times is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 2 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 2 of 1 time is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12 hours, and the mixed bacteria 2 of 2 times obtains going down to posterity; By that analogy, until the mixed bacteria 6 of 2 times that obtains going down to posterity; The mixed bacteria 6 of 2 times of going down to posterity is placed in to aseptic centrifuge tube, and at 2-6 DEG C, centrifugation obtains the lactic acid bacteria thalline of domestication, and with the fresh milk of sterilizing or reconstituted milk, concentration is washed down and be adjusted to the lactic acid bacteria thalline of described domestication is 4 × 10 ⁷-2 × 10 ⁸cfu/mL, obtains the streptococcus acidi lactici fermented solution of taming; The streptococcus acidi lactici fermented solution inoculum concentration of getting domestication is the 4-6% of fresh milk volume, after inoculation 40-42 DEG C ferment to pH be 4.5-4.8, then in 4 DEG C refrigeration 24 hours.

Claims

1. a sour milk beverage, its raw material comprises the streptococcus acidi lactici fermented solution of domestication, fresh milk.

2. a kind of sour milk beverage according to claim 1, its preparation method following steps:

A sterilizing

Fresh milk is heated to 65 DEG C--after 72 DEG C of insulation 10min, 25---under 30 MPa homogeneous once, then by Milk During Heating to 90 DEG C sterilizing 20min, be cooled to 37 DEG C for subsequent use;

The preparation of the streptococcus acidi lactici fermented solution of B domestication

The ratio that is 2: 2: 1 in thalline number by streptococcus thermophilus, lactobacillus bulgaricus and Bifidobacterium is mixed, then with 10 ⁹cfu/100mL ratio is inoculated in the sterilizing degreasing reconstituted milk of mass concentration 12%, and 37 DEG C leave standstill cultivation 12-14h and activate the mixed bacteria that obtains activation; Sterilizing after pure soya-bean milk and degreasing reconstituted milk are mixed according to the ratio of volume ratio 0: 10,1: 9,2: 8,3: 7,4: 6 and 5: 5 respectively, is designated as nutrient solution 1, nutrient solution 2, nutrient solution 3, nutrient solution 4, nutrient solution 5 and nutrient solution 6 successively; The mixed bacteria of activation is inoculated in nutrient solution 1, inoculum concentration is 3% of nutrient solution 1 volume, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 1 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 1 of 1 time is inoculated in nutrient solution 1 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 1 of 2 times obtains going down to posterity; By going down to posterity, the mixed bacteria 1 of 2 times is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill cultivation 12-14 hour, the mixed bacteria 2 of 1 time obtains going down to posterity, by going down to posterity, the mixed bacteria 2 of 1 time is inoculated in nutrient solution 2 according to the ratio of volume ratio 3%, 37 DEG C leave standstill and cultivate 12-14 hour, and the mixed bacteria 2 of 2 times obtains going down to posterity; By that analogy, until the mixed bacteria 6 of 2 times that obtains going down to posterity; The mixed bacteria 6 of 2 times of going down to posterity is placed in to aseptic centrifuge tube, and at 2-6 DEG C, centrifugation obtains the lactic acid bacteria thalline of domestication, and with the fresh milk of sterilizing or reconstituted milk, concentration is washed down and be adjusted to the lactic acid bacteria thalline of described domestication is 4 × 10 ⁷-2 × 10 ⁸cfu/mL, obtains the streptococcus acidi lactici fermented solution of taming;

C. inoculation

The streptococcus acidi lactici fermented solution inoculum concentration of getting domestication is the 4-6% of fresh milk volume, after inoculation 40-42 DEG C ferment to pH be 4.5-4.8, then in 4 DEG C refrigeration 12-24 hour.

3. according to the preparation method described in claim 2, it is characterized in that: sterilizing described in step b is 108 DEG C of insulations 20 minutes.