CN103837678A - Semiquantitative detector of luteinizing hormone - Google Patents
Semiquantitative detector of luteinizing hormone Download PDFInfo
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- CN103837678A CN103837678A CN201410104924.6A CN201410104924A CN103837678A CN 103837678 A CN103837678 A CN 103837678A CN 201410104924 A CN201410104924 A CN 201410104924A CN 103837678 A CN103837678 A CN 103837678A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/575—Hormones
- G01N2333/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
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Abstract
The invention discloses a semiquantitative detector of luteinizing hormone. The semiquantitative detector comprises a luteinizing hormone gold-labeled test strip, a power supply switch, a light emitting diode, a photoelectric sensor, a power amplifier, an A/D (Analog/Digital) converter, a singlechip, an LCD (Liquid Crystal Display) and a key; after the detector starts up, the singlechip is used for transmitting a signal to the light emitting diode, the light emitting diode is used for transmitting an optical signal to the gold-labeled test strip, the gold-labeled test strip is used for transmitting the optical signal to the photoelectric sensor, the photoelectric sensor is used for processing the optical signal into an electric signal, the electric signal is transmitted to the power amplifier, the power amplifier is used for transmitting a signal to the A/D converter, the A/D converter is used for converting the electric signal into a digital signal to form an instruction, the instruction is processed by the singlechip and then is transmitted to the LCD to display the result, and the result is stored in the singlechip, and the LCD realizes the function of drawing the detection result to be a curve. The detector has the advantages of stability, reliability and accurate test.
Description
Technical field
The half-quantitative detection instrument that the present invention relates to a kind of interstitialcellstimulating hormone (ICSH), belongs to field of medical examination, for detection of the onset of ovulation.
Background technology
Existing for detection of the onset of ovulation be mostly reaction test strips, by every day the shade of detection line being compared to judge device for testing ovulation date.Because the detection line on test paper reaction film directly contacts with air with nature controlling line, can make test solution volatilization, outer carrying liqs is easily penetrated in responding layer simultaneously, affect test result, range estimation error is large, have influence on test result, the outcome record of shade can not be got off to be depicted as curve, carry out the judgement of an onset of ovulation simultaneously.
Summary of the invention
The object of the invention is in order to overcome above deficiency, a kind of reliable and stable, robotization is provided, tests the half-quantitative detection instrument of interstitialcellstimulating hormone (ICSH) accurately.
Object of the present invention is achieved through the following technical solutions: a kind of half-quantitative detection instrument of interstitialcellstimulating hormone (ICSH), comprises interstitialcellstimulating hormone (ICSH) gold-marking test strip, power switch, light emitting diode, photoelectric sensor, power amplifier, A/D digital to analog converter, single-chip microcomputer, LCD liquid crystal display, button;
Power switch is realized power on/off function, signal is passed to light emitting diode by single-chip microcomputer, light signal is passed to gold-marking test strip by light emitting diode, light signal is passed to photoelectric sensor by gold-marking test strip, photoelectric sensor is treated to electrical signal transfer to power amplifier by light signal, signal is passed to A/D analog to digital converter by power amplifier, A/D analog to digital converter converts the electrical signal to digital signal, form an instruction, instruction is by single-chip microcomputer processing, passing to LCD liquid crystal display demonstrates result out and is stored in single-chip microcomputer, button can be realized menu function, LCD LCDs can realize the function of each testing result being drawn out to one section of curve.
Further improvement of the present invention is: photoelectric sensor adopts silicon photocell.
Further improvement of the present invention is: LED source is injected, light source is made up of incident light source, transmitted light source, because gold-marking immunity examination bar develops the color in red color range, its corresponding wavelength is about 515-555nm scope, selecting peak wavelength according to complementary light principle is 530nm light source, light source adopts light emitting diode, when tying at the P-N of light emitting diode while adding positive voltage, the diffusion motion of charge carrier is strengthened, make the electronics in atom obtain kinetic energy from the electronics there of accelerating, transit to high level by low-lying level; In the time that its excited state is returned to normal condition, electronics discharges the energy of doing more with the form of transmitting photon, produce electro optical phenomenon; The energy of photon depend on semiconductor material energy gap Eg., the energy of emitting is larger, the optical radiation wavelength sending is just shorter; Photoelectric sensor adopts the silicon photocell take silicon materials as matrix, and the principle of work of silicon photocell is light volta effect; In the time of the PN junction district of irradiation at silicon photocell, can inspire light induced electron hole in semiconductor time, the light induced electron hole pair on PN junction both sides, using of internal electric field, what belong to majority carrier can not pass through restraining barrier, and minority carrier can pass through restraining barrier; As a result, the light induced electron in P district enters N district, and the photohole in N district enters P district, makes light induced electron one hole in each district to separated; Light induced electron makes N district electronegative in the assembly in N district, and the assembly in the P district of light induced electron makes P district positively charged; Between P district and N district, produce photoproduction electromotive force; After light silicon photocell access load, photocurrent flows to N district from P district through load, obtains power stage in load.
The present invention compared with prior art has the following advantages: the present invention does not need range estimation, detect by light, be not subject to the impact of ambient light power, eyesight, robotization, test accurately, each result can be drawn out in LCD LCDs to one section of curve function, thereby be carried out the judgement of a device for testing ovulation date.
accompanying drawing explanation:
Fig. 1 is structural representation of the present invention;
Number in the figure: 1-power switch, 2-light emitting diode, 3-interstitialcellstimulating hormone (ICSH) (LH) gold-marking test strip, 4-photoelectric sensor, 5-power amplifier, 6-A/D analog to digital converter, 7-single-chip microcomputer, 8-LCD LCDs, 9-button.
embodiment:
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, and this embodiment only, for explaining the present invention, does not form limiting the scope of the present invention.
As Fig. 1 shows a kind of embodiment of the half-quantitative detection instrument of a kind of interstitialcellstimulating hormone (ICSH) of the present invention, comprise power switch 1, light emitting diode 2, interstitialcellstimulating hormone (ICSH) (LH) gold-marking test strip 3, photoelectric sensor 4, power amplifier 5, A/D analog to digital converter 6, single-chip microcomputer 7, LCD LCDs 8, button 9.Power switch 1 is realized power on/off function, signal is passed to light emitting diode 2 by single-chip microcomputer 7, light signal is passed to gold-marking test strip 3 by light emitting diode 2, light signal is passed to photoelectric sensor 4 by gold-marking test strip 3, photoelectric sensor 4 is treated to electrical signal transfer to power amplifier 5 by light signal, signal is passed to A/D analog to digital converter 6 by power amplifier 5, A/D analog to digital converter 6 converts the electrical signal to digital signal, form an instruction, instruction is processed by single-chip microcomputer 7, passing to LCD liquid crystal display 8 demonstrates result out and is stored in single-chip microcomputer 7, button 9 can be realized menu function, LCD LCDs 8 can realize the function of each testing result being drawn out to one section of curve.Photoelectric sensor 4 adopts silicon photocell.
Light emitting diode 2 light sources are injected, and light source is made up of incident light source, transmitted light source, and because gold-marking immunity examination bar 3 develops the color in red color range, its corresponding wavelength is about 515-555nm scope.Selecting peak wavelength according to complementary light principle is 530nm light source; Light source adopts light emitting diode 2, and when tying at the P-N of light emitting diode 2 while adding positive voltage, the diffusion motion of charge carrier is strengthened, and makes the electronics in atom obtain kinetic energy from the electronics there of accelerating, and transits to high level by low-lying level; In the time that its excited state is returned to normal condition, electronics discharges the energy of doing more with the form of transmitting photon, produce electro optical phenomenon.The energy of photon depend on semiconductor material energy gap E
g..The energy of emitting is larger, and the optical radiation wavelength sending is just shorter.Photoelectric sensor 4 adopts the silicon photocell take silicon materials as matrix, and the principle of work of silicon photocell is light volta effect.In the time of the PN junction district of irradiation at silicon photocell, can inspire light induced electron hole in semiconductor time.The light induced electron hole pair on PN junction both sides, using of internal electric field, what belong to majority carrier can not pass through restraining barrier, and minority carrier can pass through restraining barrier.As a result, the light induced electron in P district enters N district, and the photohole in N district enters P district, makes light induced electron one hole in each district to separated.Light induced electron makes N district electronegative in the assembly in N district, and the assembly in the P district of light induced electron makes P district positively charged.Between P district and N district, produce photoproduction electromotive force.After light silicon photocell access load, photocurrent from P district through load flow to N I, in load, obtain power stage.
The present invention does not need range estimation, detects by light, is not subject to the impact of ambient light power, eyesight, robotization, and test accurately, can be drawn out each result one section of curve function, thereby carry out the judgement of a device for testing ovulation date in LCD LCDs.
Claims (3)
1. a half-quantitative detection instrument for interstitialcellstimulating hormone (ICSH), is characterized in that: comprise interstitialcellstimulating hormone (ICSH) gold-marking test strip, power switch, light emitting diode, photoelectric sensor, power amplifier, A/D digital to analog converter, single-chip microcomputer, LCD liquid crystal display, button;
Power switch is realized power on/off function, signal is passed to light emitting diode by single-chip microcomputer, light signal is passed to gold-marking test strip by light emitting diode, light signal is passed to photoelectric sensor by gold-marking test strip, photoelectric sensor is treated to electrical signal transfer to power amplifier by light signal, signal is passed to A/D analog to digital converter by power amplifier, A/D analog to digital converter converts the electrical signal to digital signal, form an instruction, instruction is by single-chip microcomputer processing, passing to LCD liquid crystal display demonstrates result out and is stored in single-chip microcomputer, button can be realized menu function, LCD LCDs can realize the function of each testing result being drawn out to one section of curve.
2. the half-quantitative detection instrument of interstitialcellstimulating hormone (ICSH) according to claim 1, is characterized in that: described photoelectric sensor adopts silicon photocell.
3. the half-quantitative detection instrument of interstitialcellstimulating hormone (ICSH) according to claim 1, it is characterized in that: LED source is injected, light source is made up of incident light source, transmitted light source, because gold-marking immunity examination bar develops the color in red color range, its corresponding wavelength is about 515-555nm scope, selecting peak wavelength according to complementary light principle is 530nm light source, light source adopts light emitting diode, when tying at the P-N of light emitting diode while adding positive voltage, the diffusion motion of charge carrier is strengthened, make the electronics in atom obtain kinetic energy from the electronics there of accelerating, transit to high level by low-lying level; In the time that its excited state is returned to normal condition, electronics discharges the energy of doing more with the form of transmitting photon, produce electro optical phenomenon; The energy of photon depend on semiconductor material energy gap Eg., the energy of emitting is larger, the optical radiation wavelength sending is just shorter; Photoelectric sensor adopts the silicon photocell take silicon materials as matrix, and the principle of work of silicon photocell is light volta effect; In the time of the PN junction district of irradiation at silicon photocell, can inspire light induced electron hole in semiconductor time, the light induced electron hole pair on PN junction both sides, using of internal electric field, what belong to majority carrier can not pass through restraining barrier, and minority carrier can pass through restraining barrier; As a result, the light induced electron in P district enters N district, and the photohole in N district enters P district, makes light induced electron one hole in each district to separated; Light induced electron makes N district electronegative in the assembly in N district, and the assembly in the P district of light induced electron makes P district positively charged; Between P district and N district, produce photoproduction electromotive force; After light silicon photocell access load, photocurrent flows to N district from P district through load, obtains power stage in load.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410104924.6A CN103837678A (en) | 2014-03-20 | 2014-03-20 | Semiquantitative detector of luteinizing hormone |
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| CN201410104924.6A CN103837678A (en) | 2014-03-20 | 2014-03-20 | Semiquantitative detector of luteinizing hormone |
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| CN103837678A true CN103837678A (en) | 2014-06-04 |
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| CN201410104924.6A Pending CN103837678A (en) | 2014-03-20 | 2014-03-20 | Semiquantitative detector of luteinizing hormone |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105116153A (en) * | 2015-08-07 | 2015-12-02 | 南通市伊士生物技术有限责任公司 | Semiquantitative detection system for luteinizing hormone and detection method thereof |
| CN107356767A (en) * | 2017-07-28 | 2017-11-17 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) test strips analyzer |
| CN107389954A (en) * | 2017-07-28 | 2017-11-24 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) ELISA test strip pen |
| CN115327119A (en) * | 2022-10-18 | 2022-11-11 | 深圳市强大创新科技实业有限公司 | Antigen detector and detection method thereof |
Citations (4)
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| CN101839908A (en) * | 2010-05-28 | 2010-09-22 | 李金波 | Quantitative detection device and detection method of biological fluid samples |
| CN201867372U (en) * | 2010-11-30 | 2011-06-15 | 南通市伊士生物技术有限责任公司 | Semi-quantitative quick-detection electronic stylus device for colloidal-gold strip |
| CN201867374U (en) * | 2010-11-30 | 2011-06-15 | 南通市伊士生物技术有限责任公司 | Electronic pen device for gold marking test strip semi-quantitative fast detection |
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
-
2014
- 2014-03-20 CN CN201410104924.6A patent/CN103837678A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101839908A (en) * | 2010-05-28 | 2010-09-22 | 李金波 | Quantitative detection device and detection method of biological fluid samples |
| CN201867372U (en) * | 2010-11-30 | 2011-06-15 | 南通市伊士生物技术有限责任公司 | Semi-quantitative quick-detection electronic stylus device for colloidal-gold strip |
| CN201867374U (en) * | 2010-11-30 | 2011-06-15 | 南通市伊士生物技术有限责任公司 | Electronic pen device for gold marking test strip semi-quantitative fast detection |
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105116153A (en) * | 2015-08-07 | 2015-12-02 | 南通市伊士生物技术有限责任公司 | Semiquantitative detection system for luteinizing hormone and detection method thereof |
| CN105116153B (en) * | 2015-08-07 | 2017-07-07 | 南通市伊士生物技术有限责任公司 | The half-quantitative detection system and its detection method of interstitialcellstimulating hormone (ICSH) |
| CN107356767A (en) * | 2017-07-28 | 2017-11-17 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) test strips analyzer |
| CN107389954A (en) * | 2017-07-28 | 2017-11-24 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) ELISA test strip pen |
| CN115327119A (en) * | 2022-10-18 | 2022-11-11 | 深圳市强大创新科技实业有限公司 | Antigen detector and detection method thereof |
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Address after: 226000 Jiangsu city of Nantong Province, Nantong economic and Technological Development Zone, Road No. 1692 No. 15 building block A Applicant after: NANTONG EGENS BIOTECHNOLOGY CO., LTD. Address before: 226000 Jiangsu Province, Nantong City Lake Economic and Technological Development Zone, Road No. 1692 No. 15 building block A Applicant before: Nantong Egens Biotechnology Co., Ltd. |
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Application publication date: 20140604 |
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