CN103828678A - Method for quickly identifying pear rotting-resistant disease - Google Patents
Method for quickly identifying pear rotting-resistant disease Download PDFInfo
- Publication number
- CN103828678A CN103828678A CN201410079119.2A CN201410079119A CN103828678A CN 103828678 A CN103828678 A CN 103828678A CN 201410079119 A CN201410079119 A CN 201410079119A CN 103828678 A CN103828678 A CN 103828678A
- Authority
- CN
- China
- Prior art keywords
- pear
- epidermis
- hole skin
- rot
- relative area
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000014443 Pyrus communis Nutrition 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 33
- 201000010099 disease Diseases 0.000 title abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 6
- 240000001987 Pyrus communis Species 0.000 claims abstract description 32
- 210000002615 epidermis Anatomy 0.000 claims abstract description 29
- 210000003491 skin Anatomy 0.000 claims description 31
- 230000000284 resting effect Effects 0.000 claims description 12
- 208000035240 Disease Resistance Diseases 0.000 claims description 5
- 238000003384 imaging method Methods 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 10
- 238000012797 qualification Methods 0.000 description 9
- 241000220324 Pyrus Species 0.000 description 8
- 235000021017 pears Nutrition 0.000 description 8
- 229910052742 iron Inorganic materials 0.000 description 5
- 206010039509 Scab Diseases 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 206010053615 Thermal burn Diseases 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 239000001965 potato dextrose agar Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 229920000297 Rayon Polymers 0.000 description 2
- 210000003038 endothelium Anatomy 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000088401 Pyrus pyrifolia Species 0.000 description 1
- 235000001630 Pyrus pyrifolia var culta Nutrition 0.000 description 1
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明公开了一种梨树抗腐烂病快速鉴定方法，该方法利用梨树一年生休眠枝条的“皮孔相对面积”与人工接种梨树的发病程度之间存在显著的正相关这一规律，选取梨树一年生休眠枝条，通过测定枝条表皮的“皮孔相对面积”，对梨树的抗腐烂病进行分级；与现有技术相比，本发明具有快速、简便、高效的特点。The invention discloses a rapid identification method for pear tree rot resistance. The method utilizes the law that there is a significant positive correlation between the "relative area of lenticels" of annual dormant branches of pear trees and the disease degree of artificially inoculated pear trees. For annual dormant branches of pear trees, the rot resistance of pear trees is graded by measuring the "relative lenticel area" of the branch epidermis; compared with the prior art, the invention has the characteristics of fast, simple and efficient.
Description
Technical field
What the present invention relates to is a kind of authentication method of pear tree disease, in particular the anti-rot rapid identification method of a kind of pear tree.
Background technology
Fungus of Pear Canker Disease all has generation in the each main producing region of China's pears, occurs heavier with Xinjiang, northwest, North China and other places.According to current investigation, Xinjiang Kuerle delicious pear diseased plant rate has reached 50%～80%, and the crisp pears diseased plant rate in the Northwest reaches 30%～50%; North China's pear, Chinese pear diseased plant rate all reach more than 30%.Rot has that generation area is wide, the incidence of disease is high, unmanageable feature, the serious theatre of falling ill, and the sick scar of tree body is innumerable, limb is incomplete, even causes a large amount of Si Shuhuohui garden, and serious threat is to the sound development of China's pears industry.
Exploitation by new technology and multiple technologies integrated, sets up continuable pears rot Comprehensive Preventing control techniques system for planted in different ecological areas, promotes China's pears rot prevention and control technical merit although can be.But, cultivate and filter out the improved Varieties of anti-rot and carry out spread, be the only way which must be passed of effectively controlling Fungus of Pear Canker Disease and spreading the safety in production that increases the weight of, ensures China's pears.At present, the resistance of the different quality resources of qualification pear tree to rot, only gets annual resting shoot sample, adopts electric iron to scald Vitro Inoculation Technique, or field nursery stock is carved and hinders in-situ inoculating.Like this, not only need a large amount of bacterium source of activation culture, after inoculation, still need and wait for about one week, could statistical results.As can be seen here, for the anti-rot characterization and evaluation of pear tree, still lack quick, easy, effective method.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of pear tree anti-rot rapid identification method, to provide a kind of pear tree quick, easy, that effective method identifies high resistance type anti-rot kind.
The present invention is achieved by the following technical solutions, the present invention includes following steps:
The anti-rot rapid identification method of a kind of pear tree, comprises the following steps:
(1) get the annual resting shoot of pear tree, peel the epidermis of branch, measure supracutaneous hole skin and account for the area ratio of whole epidermis, obtain " hole skin relative area ";
(2) disease resistance classification: the Pear Trees with Various Cultivars of " hole skin relative area "≤2% is high resistance type, " hole skin relative area " Pear Trees with Various Cultivars of (2%, 6%) is intermediate form in open interval, the Pear Trees with Various Cultivars of " hole skin relative area " >=6% is susceptible type.
Preferably, in described step (1), annual resting shoot is the annotinous branch to the tree crown periphery before rudiment after pear tree fallen leaves.
Preferably, in described step (1), the middle part that the sampling point of annual resting shoot is branch.
Preferably, in described step (1), the area that strips of epidermis is 1～2 square centimeter.
Preferably, in described step (1), the assay method of " hole skin relative area " is: first with the above precision scanning of 300dpi epidermis, preserve imaging, measure total pixel value of epidermis and total pixel value of hole skin, the ratio of total pixel value of hole skin and total pixel value of epidermis is " hole skin relative area ".
Operation principle of the present invention is: the present invention has chosen various types of kind and represented 38 parts of resources (deriving from 12 kinds of pear), by traditional electrical flatiron scald, the resistance of Vitro Inoculation Technique method qualification different cultivars resource to rot, carry out correlation analysis by the measurement result of the aspect indexs such as morphology, cytology, physiology and molecular biology again,, between " the hole skin relative area " of the annual resting shoot of pear tree and the occurring degree of artificial infection pear tree, there is significant positive correlation (its correlation coefficient r=0.9381) in final discovery.Therefore, established the feasibility that substitutes the resistance level of traditional inoculation method qualification pear tree different cultivars resource to rot with " hole skin relative area ".
The present invention has the following advantages compared to existing technology: the invention provides the anti-rot rapid identification method of a kind of pear tree, the method only need strip the epidermis of the annual resting shoot of pear tree, measure " hole skin relative area ", can, by anti-pear tree rot qualitative classification, there is quick, easy, efficient feature; And traditional electric iron is scalded, Vitro Inoculation Technique method, before inoculation, need to activate a large amount of bacterial classifications, the branch quantity that inoculation needs is many, needs to cultivate and within 7～10 days, just can observe qualification after inoculation, and qualification cycle is long.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented under taking technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
The present embodiment is chosen fresh pears in June, Nan Guoli and Pucheng three kinds of Pear Trees with Various Cultivars of snow and is represented resource, carries out anti-rot Rapid identification, comprises the following steps:
(1) get respectively after fallen leaves to the peripheral annual resting shoot of tree crown of the above-mentioned two kinds of Pear Trees with Various Cultivars before rudiment, peel the epidermis at branch middle part, described epidermis strip approximately 1 square centimeter of area, epidermis is fixed on blank sheet of paper with transparent viscose glue, repeat 5 times;
A, be fixed on the epidermis on blank sheet of paper with the scanner scanning of 300bpi precision, preserve imaging;
B, utilization photoshop image processing software, choose with square selection tool the unit epidermis area that hole skin is evenly distributed, and checks in the pixel value Sa of this unit epidermis area in " histogram " column in " image " menu;
" brightness " and " contrast " of c, increase image, select " magic wand menu ", adjust " tolerance " value, make " magic wand menu " just in time can select all hole skins and be advisable, then in " histogram " column in " image " menu, check in the pixel value La of this unit epidermis area endothelium hole area;
" hole skin relative area " RA(% of d, calculating branch epidermis), RA=La/Sa × 100%;
(2) disease resistance classification:
June fresh pears: La=806, Sa=43818, RA=1.84%, is high resistance type;
Nan Guoli: La=2436, Sa=43820, RA=5.56%, is intermediate form;
Pucheng snow: La=4710, Sa=44019, RA=10.70%, is susceptible type.
Embodiment 2
The present embodiment is chosen 38 parts of Pear Trees with Various Cultivars and is represented resource, relates to 12 kinds and a species hybrid in country variant and area, identifies the anti-rot degree of these 38 parts of Pear Trees with Various Cultivars resources, comprises the following steps:
(1) choose above-mentioned 38 parts of Pear Trees with Various Cultivars December and represent resource, get 3 of the peripheral annual resting shoots of tree crown, peel branch middle part epidermis epidermis strip approximately 2 square centimeters of areas, epidermis is fixed on blank sheet of paper with transparent viscose glue;
A, be fixed on the epidermis on blank sheet of paper with the scanner scanning of 300bpi precision, preserve imaging;
B, utilization photoshop image processing software, choose with square selection tool the unit epidermis area that hole skin is evenly distributed, and checks in the pixel value Sa of this unit epidermis area in " histogram " column in " image " menu;
" brightness " and " contrast " of c, increase image, select " magic wand menu ", adjust " tolerance " value, make " magic wand menu " just in time can select all hole skins and be advisable, then in " histogram " column in " image " menu, check in the pixel value La of this unit epidermis area endothelium hole area;
" hole skin relative area " RA(% of d, calculating branch epidermis), RA=La/Sa × 100%;
(2) disease resistance classification: the Pear Trees with Various Cultivars of " hole skin relative area "≤2% is high resistance type, " hole skin relative area " is in open interval (2%, 6%) Pear Trees with Various Cultivars is intermediate form, and the Pear Trees with Various Cultivars of " hole skin relative area " >=6% is susceptible type, and qualification result is as shown in table 1.
Meanwhile, the peripheral annual resting shoot of tree crown that represents resource taking above-mentioned 38 parts of Pear Trees with Various Cultivars, as test material, adopts electric iron scald, Vitro Inoculation Technique method as a control group.Every part of resource is got 5 of long 30～40 centimetres of branch sections, on the branch skin of every branch section, poker the wound of 3 diameter 5～7mm with electric iron, wound depth reaches xylem, the rotten pathogenic bacteria cake that wherein potato dextrose agar PDA cultivation 4 days for the inoculation of the wound of 4 branches, punching diameter are 5mm, another root branch wound is inoculated in contrast with aseptic PDA.Each branch section of processing is put in plastic preserving box, and aseptic moistening gauze moisturizing, was placed in 25 DEG C of constant temperature culture after 3 days, observed moisturizing and incidence every 24 hours, after inoculation, 10d measures expansion scab diameter, calculates each kind scab " spreading coefficient " Ec, represents each kind inoculation occurring degree:
Ec=∑ (expansion scab diameter-electric iron punching diameter)/morbidity hole count.
According to vaccination scab spread scenarios, these 38 parts of Pear Trees with Various Cultivars are represented to resource carries out disease resistance classification, be divided into altogether Three Estate:
High resistance type: Ec≤6;
Intermediate form: 6<Ec<50;
Susceptible type: Ec >=50
The present invention and traditional inoculated identification result are as shown in table 1 below:
Table 1: Pear Trees with Various Cultivars represents the anti-rot qualification result of resource
In table 1, can find out, the qualification result of the method for the invention and traditional electrical flatiron are scalded, the qualification result of Vitro Inoculation Technique method is in full accord, and recall rate reaches 100%; On the other hand, 7～10 days consuming time of traditional authentication method, only has about 1 hour and the method for the invention is consuming time, and therefore, than conventional method, the method for the invention advantage is remarkable.
Claims (5)
1. the anti-rot rapid identification method of pear tree, is characterized in that, comprises the following steps:
(1) get the annual resting shoot of pear tree, peel the epidermis of branch, measure supracutaneous hole skin and account for the area ratio of whole epidermis, obtain " hole skin relative area ";
(2) disease resistance classification: the Pear Trees with Various Cultivars of " hole skin relative area "≤2% is high resistance type, " hole skin relative area " Pear Trees with Various Cultivars of (2%, 6%) is intermediate form in open interval, the Pear Trees with Various Cultivars of " hole skin relative area " >=6% is susceptible type.
2. the anti-rot rapid identification method of a kind of pear tree as claimed in claim 1, is characterized in that, in described step (1), annual resting shoot is the annotinous branch to the tree crown periphery before rudiment after pear tree fallen leaves.
3. the anti-rot rapid identification method of a kind of pear tree as described in as arbitrary in claim 1 or 2, is characterized in that, in described step (1), and the middle part that the sampling point of annual resting shoot is branch.
4. the anti-rot rapid identification method of a kind of pear tree as claimed in claim 1, is characterized in that, in described step (1), the area that strips of epidermis is 1～2 square centimeter.
5. the anti-rot rapid identification method of a kind of pear tree as claimed in claim 1, it is characterized in that, in described step (1), the assay method of " hole skin relative area " is: first with the above precision scanning of 300dpi epidermis, preserve imaging, measure total pixel value of epidermis and total pixel value of hole skin, the ratio of total pixel value of hole skin and total pixel value of epidermis is " hole skin relative area ".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410079119.2A CN103828678B (en) | 2014-03-05 | 2014-03-05 | The anti-rot rapid identification method of a kind of pear tree |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410079119.2A CN103828678B (en) | 2014-03-05 | 2014-03-05 | The anti-rot rapid identification method of a kind of pear tree |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103828678A true CN103828678A (en) | 2014-06-04 |
| CN103828678B CN103828678B (en) | 2015-08-12 |
Family
ID=50792834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410079119.2A Expired - Fee Related CN103828678B (en) | 2014-03-05 | 2014-03-05 | The anti-rot rapid identification method of a kind of pear tree |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103828678B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107517726A (en) * | 2017-10-14 | 2017-12-29 | 中国科学院新疆生态与地理研究所 | A kind of fast quick access bacterium of canker of apple fruit pathogen and the method for pathogenicity Rapid identification |
| CN109001368A (en) * | 2018-06-11 | 2018-12-14 | 塔里木大学 | A method of detection Kuerle delicious pear tree infects rot disease |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640671A (en) * | 2012-05-02 | 2012-08-22 | 湖北省农业科学院果树茶叶研究所 | Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm |
| CN102719559A (en) * | 2012-06-12 | 2012-10-10 | 中国检验检疫科学研究院 | Chip for screening avsunviroid viroid and application of chip |
| CN103371062A (en) * | 2012-04-17 | 2013-10-30 | 河北省农林科学院昌黎果树研究所 | In-vitro identification method for venturia nashicola resistance |
| CN103503737A (en) * | 2013-10-09 | 2014-01-15 | 中国农业科学院郑州果树研究所 | Method for identifying resistance of cytosporacarphosperma |
-
2014
- 2014-03-05 CN CN201410079119.2A patent/CN103828678B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371062A (en) * | 2012-04-17 | 2013-10-30 | 河北省农林科学院昌黎果树研究所 | In-vitro identification method for venturia nashicola resistance |
| CN102640671A (en) * | 2012-05-02 | 2012-08-22 | 湖北省农业科学院果树茶叶研究所 | Method for identifying Alternaria altemata (Fr.) Keissler resistance of pear germplasm |
| CN102719559A (en) * | 2012-06-12 | 2012-10-10 | 中国检验检疫科学研究院 | Chip for screening avsunviroid viroid and application of chip |
| CN103503737A (en) * | 2013-10-09 | 2014-01-15 | 中国农业科学院郑州果树研究所 | Method for identifying resistance of cytosporacarphosperma |
Non-Patent Citations (2)
| Title |
|---|
| 张翠香等: "加强防治梨树腐烂病", 《山西农业(致富科技)》, 31 December 2006 (2006-12-31) * |
| 段志坤: "南方梨树几种常见枝干病虫及其防治方法", 《果农之友》, 31 October 2010 (2010-10-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107517726A (en) * | 2017-10-14 | 2017-12-29 | 中国科学院新疆生态与地理研究所 | A kind of fast quick access bacterium of canker of apple fruit pathogen and the method for pathogenicity Rapid identification |
| CN109001368A (en) * | 2018-06-11 | 2018-12-14 | 塔里木大学 | A method of detection Kuerle delicious pear tree infects rot disease |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103828678B (en) | 2015-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bakys et al. | Root rot, associated fungi and their impact on health condition of declining Fraxinus excelsior stands in Lithuania | |
| De Leon et al. | Lentinus squarrosulus and Polyporus grammocephalus: newly domesticated, wild edible macrofungi from the Philippines | |
| CN104126416A (en) | Phlebopus portentosu good character domestication method | |
| Herawati et al. | Domestication and nutrient analysis of Schizopyllum commune, alternative natural food sources in East Kalimantan | |
| JP2015517812A5 (en) | ||
| CN101608213A (en) | A method for identifying the mating type gene of shimeji mushroom | |
| CN102239779B (en) | Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease | |
| McCarthy | The herbaceous layer of eastern old-growth deciduous forests | |
| Barge et al. | Systematics of the ectomycorrhizal genus Lactarius in the Rocky Mountain alpine zone | |
| Ranjbar et al. | Evaluation of substrate components on shiitake mushroom properties | |
| CN103828678A (en) | Method for quickly identifying pear rotting-resistant disease | |
| CN105300895A (en) | Method for performing early warning against potato germination defects by utilizing feature point tangent included angles | |
| CN104480191A (en) | Method for rapidly identifying resistance of apple branches and trunks to ring rot | |
| CN102061330A (en) | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria | |
| Ji et al. | A report on canker disease of Falcataria moluccana caused by Lasiodiplodia theobromae in China | |
| CN105424622B (en) | A kind of method using feature triangle area early warning potato sprouting | |
| CN106305136A (en) | Cut-log tremella cultivation method | |
| Jenkins | Sclerotinia laxa Aderh. & Ruhl: a cause of brown rot of stone fruits not previously recorded in Australia | |
| Yue-Lian et al. | Identification and cultivation of a wild mushroom from banana pseudo-stem sheath | |
| CN102293107B (en) | Method for rapidly identifying black spot resistance of radish germplasms | |
| Terhonen | First report of Diplodia tip blight on Scots pine in Finland | |
| Pasailiuk et al. | Influence of rutin on fruiting of Hericium coralloides, Polyporus umbellatus, and Flammulina velutipes | |
| Ma et al. | First report of Neofusicoccum parvum causing leaf spot on coral trees (Viburnum odoratissimum) in China | |
| SONCHITA | Diversity, distribution and morphological characterization of wild macro fungi from gajni Forest | |
| Saadullah et al. | Contamination of dried figs with fungi and aflatoxigenic potential of some isolates of Aspergillus section Flavi |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20160305 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |