CN103822923B - A kind of method identifying the Lignum Aquilariae Resinatum true and false - Google Patents
A kind of method identifying the Lignum Aquilariae Resinatum true and false Download PDFInfo
- Publication number
- CN103822923B CN103822923B CN201410060370.4A CN201410060370A CN103822923B CN 103822923 B CN103822923 B CN 103822923B CN 201410060370 A CN201410060370 A CN 201410060370A CN 103822923 B CN103822923 B CN 103822923B
- Authority
- CN
- China
- Prior art keywords
- lignum aquilariae
- aquilariae resinatum
- false
- water
- true
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of method identifying the Lignum Aquilariae Resinatum true and false.Carry out as steps described below: (1) cuts pat from the Lignum Aquilariae Resinatum to identify；(2) decocting in water removes the air in sample；(3) put in softening agent and soften；(4) section is with transparent；(5) Microscopic observation is amplified.The present invention is easy to operate, equipment that need not be expensive not only, and the crowd of being suitable for is wide.Can carry out the qualification of the Lignum Aquilariae Resinatum true and false by this method often in contact with the expert of Lignum Aquilariae Resinatum, more rare, the new hand just having contacted Lignum Aquilariae Resinatum can also utilize this method that Lignum Aquilariae Resinatum is identified.This method can for ensureing Lignum Aquilariae Resinatum quality, reduce false pseudo-Lignum Aquilariae Resinatum and specification Lignum Aquilariae Resinatum market etc. to provide technical guarantee.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of method identifying the Lignum Aquilariae Resinatum true and false.
Background technology
Lignum Aquilariae Resinatum refers to the timber that thymelaeceae eaglewood contains resin.Lignum Aquilariae Resinatum cannot be generated in eaglewood normal growth situation, only just have an opportunity after being subject to external injury near its wound and produce resin formation Lignum Aquilariae Resinatum, the usual Edgeworthia chrysantha Lindl. phase is extremely very long, it is necessary to many decades is even gone up the time of a century, thus the yield of Lignum Aquilariae Resinatum is rare.Although yielding poorly of Lignum Aquilariae Resinatum, but it has the huge market demand in multiple fields such as medical treatment, spice, religion and collections.Lignum Aquilariae Resinatum can, as medical material, be the main component of over one hundred kind of Chinese patent medicine；Lignum Aquilariae Resinatum is also used as spice, is referred to as " kings of ten thousand perfume (or spice) "；The treasure that Lignum Aquilariae Resinatum or religion praise highly, using Lignum Aquilariae Resinatum of burning as giving the highest form visiing the Holy Ghost；Lignum Aquilariae Resinatum is also used as collectibles, is identity and status symbol.
Lignum Aquilariae Resinatum is rare, and demand is big, so Lignum Aquilariae Resinatum price climbs up and up, under the ordering about of economic interests, illegal people utilizes various means to make and sell false Lignum Aquilariae Resinatum, thus common on market pass a fake product off as a genuine one, shoddy phenomenon.So how to identify the true and false of Lignum Aquilariae Resinatum, be essentially all by experience at present.Sum up a set of method differentiating Lignum Aquilariae Resinatum often in contact with the expert of Lignum Aquilariae Resinatum: at a glance, two touch, three hear, four burnings.But this method is not suitable for just contact or contacts the people that Lignum Aquilariae Resinatum is few.The first is because a multitude of names of Lignum Aquilariae Resinatum, and for just contacting the people of Lignum Aquilariae Resinatum, light hears that these prefixs are all dizzy, let alone authenticated；Two genuine Lignum Aquilariae Resinatum is little on the market for it, it was reported that Lignum Aquilariae Resinatum 90% is fake products on the market, the Lignum Aquilariae Resinatum of quality better phoenix feathers and unicorn horns especially, and without meeting real Lignum Aquilariae Resinatum, what carrys out experience, differentiates just not know where to begin.
Summary of the invention
It is an object of the invention to spread unchecked for false Lignum Aquilariae Resinatum at present and only differentiate the severe situation of Lignum Aquilariae Resinatum by experience, it is provided that a kind of method differentiating the Lignum Aquilariae Resinatum true and false of simple possible.Namely this method is applicable to the expert often in contact with Lignum Aquilariae Resinatum, is also applied for just contacting the new hand of Lignum Aquilariae Resinatum.This method is for reducing false pseudo-Lignum Aquilariae Resinatum, and the aspect such as specification Lignum Aquilariae Resinatum market has great importance.
A kind of method identifying the Lignum Aquilariae Resinatum true and false, is characterized in that: identifies Lignum Aquilariae Resinatum true and false method, comprises the following steps:
1) sampling, 2) decocting in water catches up with gas, and 3) softening, 4) section and 5) observe.
Described sampling: when not affecting Lignum Aquilariae Resinatum overall appearance, cuts one piece of square sample with the icking tool inner surface from Lignum Aquilariae Resinatum or the back side；
Described decocting in water catches up with gas: the material that will obtain in step 1), inserts in water, repeatedly boils several times, until sample sinks；
Described softening: by step 2) in material, put in softening agent 3～4 hours, softening is to easily cutting out full sheet.
Described section: by the material in step 3), be cut into the thin slice of about 0.1mm, inserts in chloral hydrate clarifier by thin slice, and 40 DEG C transparent 4～8 hours.
Described observation: by the thin slice in clarifier, is placed on microscope slide, is placed under magnifier, sees whether to meet the typical feature of following Lignum Aquilariae Resinatum: circular or polygonal cavity is conduit, and often 2～10 exist in groups, containing brown resin in catheter segment；The parenchyma cell group of island shape is interxylary phloem, includes brown resin；1 row, the cell containing rosthornite of even 2 row that intersect with interxylary phloem are xylem ray.
Described softening agent be 30% hydrogen peroxide, glacial acetic acid, water by 1:1:2 volume ratio mix form.
Described chloral hydrate clarifier is chloral hydrate, water and glycerol, dissolves by the weight ratio of 5:1.5:1 and mixes.
The invention has the beneficial effects as follows: the present invention is easy to operate, equipment that need not be expensive not only, and the crowd of being suitable for is wide.Can carry out the qualification of the Lignum Aquilariae Resinatum true and false by this method often in contact with the expert of Lignum Aquilariae Resinatum, more rare, the new hand just having contacted Lignum Aquilariae Resinatum can also utilize this method that Lignum Aquilariae Resinatum is identified.This method can for ensureing Lignum Aquilariae Resinatum quality, reduce false pseudo-Lignum Aquilariae Resinatum and specification Lignum Aquilariae Resinatum market etc. to provide technical guarantee.
Detailed description of the invention
Following example are further illustrating the present invention, rather than limitation of the present invention.
Embodiment 1: the qualification of a kind of Sliced
(1) preparation of reagent: softening agent prepare: taking 30% hydrogen peroxide and each 25ml of glacial acetic acid, add water 50ml, mixing, standby；Clarifier prepare: taking chloral hydrate 10g, add water 3g and glycerol 2g, dissolve, standby；
(2) sampling: cut 5 one piece square of samples from Sliced with icking tool；
(3) decocting in water catches up with gas: the sample cut, and inserts in water, boils 3 times on electromagnetic oven, and every minor tick 5 minutes makes sample sink；
(4) softening: by the material in step 3), put in the softening agent configured in step 1), soften 3 hours；
(5) section: by the material in step 3), being cut into the thin slice of about 0.1mm, insert in chloral hydrate clarifier with tweezers by thin slice, 40 DEG C transparent 4 hours；
(6) observe: with tweezers gently by the thin slice in clarifier, be placed on microscope slide, be placed under magnifier, observe.The typical characteristic that this kind of Sliced has Lignum Aquilariae Resinatum is genuine piece；The typical feature of described Lignum Aquilariae Resinatum: circular or polygonal cavity is conduit, and often 2 ~ 10 exist in groups, containing brown resin in catheter segment；The parenchyma cell group of island shape is interxylary phloem, includes brown resin；1 row, the cell containing rosthornite of even 2 row that intersect with interxylary phloem are xylem ray.
Embodiment 2: the qualification of a kind of Lignum Aquilariae Resinatum
(1) preparation of reagent: softening agent prepare: taking 30% hydrogen peroxide and each 25ml of glacial acetic acid, add water 50ml, mixing, standby；Clarifier prepare: taking chloral hydrate 10g, add water 3g and glycerol 2g, dissolve, standby;
(2) sampling: with icking tool, Lignum Aquilariae Resinatum is cut open from centre, cut one piece of sample square for 5mm from centre;
(3) decocting in water catches up with gas: the sample cut, and inserts in water, boils 4 times on electromagnetic oven, and every minor tick 5 minutes makes sample sink；
(4) softening: by the material in step 3), put in the softening agent of 15% hydrogen peroxide and 50% glacial acetic acid mixed in equal amounts, soften 3 hours；
5) section: with sharp icking tool, by the material in step 3), being cut into the thin slice of 0.1mm, insert in chloral hydrate clarifier with tweezers by thin slice, 40 DEG C transparent 6 hours；
6) observe: with tweezers gently by the thin slice in clarifier, be placed on microscope slide, be placed under magnifier, observe.One layer of this kind of Lignum Aquilariae Resinatum only periphery has the typical feature of Lignum Aquilariae Resinatum, does not have resin, have shoddy possibility in the timber of its internal layer.
Embodiment 3: the qualification of a kind of Lignum Aquilariae Resinatum bangle
(1) preparation of reagent: softening agent prepare: taking 30% hydrogen peroxide and each 25ml of glacial acetic acid, add water 50ml, mixing, standby；Clarifier prepare: taking chloral hydrate 10g, add water 3g and glycerol 2g, dissolve, standby.
(2) sampling: randomly select a chain strain from Lignum Aquilariae Resinatum bangle to be identified, cut with icking tool, choose half therein as sample；
(3) decocting in water catches up with gas: the sample that will choose, and inserts in water, boils 4 times on electromagnetic oven, and every minor tick 8 minutes makes sample sink；
(4) softening: by the material in step 3), put in the softening agent of 15% hydrogen peroxide and 50% glacial acetic acid mixed in equal amounts, soften 4 hours；
5) section: with sharp icking tool, by the material in step 3), being cut into the thin slice of 0.1mm, insert in chloral hydrate clarifier with tweezers by thin slice, 40 DEG C transparent 7 hours；
6) observe: with tweezers gently by the thin slice in clarifier, be placed on microscope slide, be placed under magnifier, observe.It is genuine piece that this Lignum Aquilariae Resinatum bangle has the typical architectural feature of Lignum Aquilariae Resinatum.
Embodiment 4: the qualification of a kind of Lignum Aquilariae Resinatum bead string
(1) preparation of reagent: softening agent prepare: taking 30% hydrogen peroxide and each 25ml of glacial acetic acid, add water 50ml, mixing, standby；Clarifier prepare: taking chloral hydrate 10g, add water 3g and glycerol 2g, dissolve, standby；
(2) sampling: randomly select a chain strain from Lignum Aquilariae Resinatum bangle to be identified, cut with icking tool, choose half therein as sample；
(3) decocting in water catches up with gas: the sample that will choose, and inserts in water, boils 3 times on electromagnetic oven, and every minor tick 5 minutes makes sample sink；
(4) softening: by the material in step 3), put in the softening agent of 15% hydrogen peroxide and 50% glacial acetic acid mixed in equal amounts, soften 4 hours；
5) section: with sharp icking tool, by the material in step 3), being cut into the thin slice of about 0.1, insert in chloral hydrate clarifier with tweezers by thin slice, 40 DEG C transparent 8 hours；
6) observe: with tweezers gently by the thin slice in clarifier, be placed on microscope slide, be placed under magnifier, observe.This Lignum Aquilariae Resinatum bead string does not have the typical architectural feature of Lignum Aquilariae Resinatum, is false Lignum Aquilariae Resinatum.
Claims (1)
1. the method identifying the Lignum Aquilariae Resinatum true and false, is characterized in that: identifies Lignum Aquilariae Resinatum true and false method, comprises the following steps:
1) sampling, 2) decocting in water catches up with gas, and 3) softening, 4) section and 5) observe；
Described sampling: when not affecting Lignum Aquilariae Resinatum overall appearance, cuts one piece of square sample with the icking tool inner surface from Lignum Aquilariae Resinatum or the back side；
Described decocting in water catches up with gas: the material that will obtain in step 1), inserts in water, repeatedly boils several times, until sample sinks；
Described softening: by step 2) in material, put in softening agent 3～4 hours, softening is to easily cutting out full sheet；
Described section: by the material in step 3), be cut into the thin slice of 0.1mm, inserts in chloral hydrate clarifier by thin slice, and 40 DEG C transparent 4～8 hours；
Described observation: by the thin slice in clarifier, is placed on microscope slide, is placed under magnifier, sees whether to meet the typical feature of following Lignum Aquilariae Resinatum: circular or polygonal cavity is conduit, and often 2～10 exist in groups, containing brown resin in catheter segment；The parenchyma cell group of island shape is interxylary phloem, includes brown resin；1 row, the cell containing rosthornite of even 2 row that intersect with interxylary phloem are xylem ray；
Described softening agent be 30% hydrogen peroxide, glacial acetic acid, water by 1:1:2 volume ratio mix form；
Described chloral hydrate clarifier is chloral hydrate, water and glycerol, dissolves by the weight ratio of 5:1.5:1 and mixes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060370.4A CN103822923B (en) | 2014-02-24 | 2014-02-24 | A kind of method identifying the Lignum Aquilariae Resinatum true and false |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060370.4A CN103822923B (en) | 2014-02-24 | 2014-02-24 | A kind of method identifying the Lignum Aquilariae Resinatum true and false |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103822923A CN103822923A (en) | 2014-05-28 |
| CN103822923B true CN103822923B (en) | 2016-06-29 |
Family
ID=50758091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410060370.4A Active CN103822923B (en) | 2014-02-24 | 2014-02-24 | A kind of method identifying the Lignum Aquilariae Resinatum true and false |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103822923B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699343B (en) * | 2016-01-05 | 2018-08-14 | 华南农业大学 | A kind of method of agalloch eaglewood true and false Undamaged determination |
| CN110006894A (en) * | 2018-12-13 | 2019-07-12 | 康美药业股份有限公司 | A kind of differentiating method of caulis piperis futokadsurae and Caulis et Folium piperis |
| CN117664974A (en) * | 2024-02-01 | 2024-03-08 | 中国林业科学研究院热带林业研究所 | Method for rapidly identifying Qilan agilawood and common agilawood based on tissue dissection in seedling stage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520111A (en) * | 2011-12-16 | 2012-06-27 | 西藏奇正藏药股份有限公司 | Method for detecting eight-flavour agilawood preparation |
| CN102818782A (en) * | 2012-09-04 | 2012-12-12 | 中国医学科学院药用植物研究所海南分所 | Determination method of total 2-(2-phenethyl) chromone compound content |
-
2014
- 2014-02-24 CN CN201410060370.4A patent/CN103822923B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520111A (en) * | 2011-12-16 | 2012-06-27 | 西藏奇正藏药股份有限公司 | Method for detecting eight-flavour agilawood preparation |
| CN102818782A (en) * | 2012-09-04 | 2012-12-12 | 中国医学科学院药用植物研究所海南分所 | Determination method of total 2-(2-phenethyl) chromone compound content |
Non-Patent Citations (4)
| Title |
|---|
| "一种伪品沉香的鉴别";卫爱黎 等;《中国民族民间医药杂志》;20061231(第82期);第284-287页 * |
| "沉香与一种沉香伪品的鉴别研究";童娅 等;《时珍国医国药》;20061231;第17卷(第12期);第2552-2553页 * |
| "沉香的资源、栽培与鉴别研究进展";李红念 等;《亚太传统医药》;20110228;第7卷(第2期);第134-136页 * |
| "香港商品伪沉香鉴别研究";黄海波 等;《广东药学》;20021231;第12卷(第4期);第5-6页"2.2显微鉴别"部分、"3结果"部分、图1-2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103822923A (en) | 2014-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jabaily et al. | Historical biogeography and life-history evolution of Andean Puya (Bromeliaceae) | |
| CN103822923B (en) | A kind of method identifying the Lignum Aquilariae Resinatum true and false | |
| De Vargas et al. | Pleistocene adaptive radiation in Globorotalia truncatulinoides: genetic, morphologic, and environmental evidence | |
| Van de Putte et al. | Lactarius volemus sensu lato (Russulales) from northern Thailand: morphological and phylogenetic species concepts explored | |
| Thongklang et al. | Morphological and molecular characterization of three Agaricus species from tropical Asia (Pakistan, Thailand) reveals a new group in section Xanthodermatei | |
| Harris et al. | Thalassiosira species (Bacillariophyceae) from a Scottish sea-loch | |
| Zhao et al. | Genetic diversity and discrimination of Chimonanthus praecox (L.) Link germplasm using ISSR and RAPD markers | |
| Abdelaziz et al. | Using complementary techniques to distinguish cryptic species: a new Erysimum (Brassicaceae) species from North Africa | |
| Gaonkar et al. | Two new species in the Chaetoceros socialis complex (Bacillariophyta): C. sporotruncatus and C. dichatoensis, and characterization of its relatives, C. radicans and C. cinctus | |
| Cornejo et al. | Multi‐gene phylogeny of the genus Lobaria: Evidence of species‐pair and allopatric cryptic speciation in East Asia | |
| Schechtman et al. | Dermatoscopic fi ndings as a complementary tool in the differential diagnosis of the etiological agent of tinea capitis | |
| Breman et al. | DNA barcoding and evolutionary relationships in Accipiter Brisson, 1760 (Aves, Falconiformes: Accipitridae) with a focus on African and Eurasian representatives | |
| Husemann et al. | Phylogenetic analyses of band‐winged grasshoppers (Orthoptera, Acrididae, Oedipodinae) reveal convergence of wing morphology | |
| Shelton et al. | Exploring the fossil history of pleurocarpous mosses: Tricostaceae fam. nov. from the Cretaceous of Vancouver Island, Canada | |
| Zamora et al. | Understanding lichenicolous heterobasidiomycetes: new taxa and reproductive innovations in Tremella sl | |
| Eberhardt et al. | Adventurous cuisine in Laos: Hebeloma parvisporum, a new species in Hebeloma section Porphyrospora | |
| Breedy et al. | A new species of the genus Eugorgia (Cnidaria: Octocorallia: Gorgoniidae) from mesophotic reefs in the eastern Pacific | |
| Kumar et al. | Rubrioxytricha guamensis nov. spec.(Ciliophora, Spirotricha), a novel hypotrich ciliate from Guam (United States), Micronesia | |
| Ortega et al. | Morphological and ITS identification of Cortinarius species (section Calochroi) collected in Mediterranean Quercus woodlands | |
| Sunesen et al. | Morphology, genetics and toxin profile of Prorocentrum texanum (Dinophyceae) from Argentinian marine coastal waters | |
| Biketova et al. | Reappraisal of the genus Exsudoporus (Boletaceae) worldwide based on multi-gene phylogeny, morphology and biogeography, and insights on Amoenoboletus | |
| Cobos-Villagrán et al. | Three new species of Rhytidhysteron (Dothideomycetes, Ascomycota) from Mexico | |
| Maslova et al. | Diverse epiphyllous fungi on Cunninghamia leaves from the Oligocene of South China and their paleoecological and paleoclimatic implications | |
| Hayes et al. | Consequences of Corymbia (Myrtaceae) hybridisation on leaf-oil profiles | |
| CN101943665B (en) | Method for detecting quality of wild jujube barks |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |