CN102520111A

CN102520111A - Method for detecting eight-flavour agilawood preparation

Info

Publication number: CN102520111A
Application number: CN2011104242951A
Authority: CN
Inventors: 续艳丽; 包旭宏; 张樱山; 陈丽娟; 张国霞
Original assignee: Tibet Cheezheng Tibetan Medicine Co Ltd
Current assignee: Tibet Cheezheng Tibetan Medicine Co Ltd
Priority date: 2011-12-16
Filing date: 2011-12-16
Publication date: 2012-06-27

Abstract

The invention discloses a method for detecting an eight-flavour agilawood preparation, which has the advantages of being strong in specificity, good in repeatability and simple and convenient for operation. The detection method comprises microscopic identification and thin-layer identification. On the basis of the primary standard, specificity thin-layer identification methods of elecampane and myrobalan are newly increased; found from a lot of thin-layer identification tests, a unified method and a developer are adopted for identifying frankincense and elecampane; and, compared with the primary standard, the repeatability and the specificity are stronger.

Description

The detection method of Bawei Chenxiang preparation

Technical field:

The present invention relates to a kind of quality determining method of pharmaceutical composition, particularly the quality determining method of Bawei Chenxiang preparation belongs to Chinese medicine detection technique field.

Background technology

The Bawei Chenxiang ball is a Tibetan medicine, records in " Drug Standard of Ministry of Public Health of the Peoples Republic of China " Tibetan medicine fascicle, and standard is numbered WS3-BC-0235-95.Agalloch eaglewood: record one one of Chinese Pharmacopoeia version in 2010, the 172nd page, these article contain the timber of resin for fragrant Aq μ ilaria sinensis (the Lo μ r.) Gilg of Thymelaeceae plant whitewood.All can gather the whole year, extracts resiniferous timber, removes not resiniferous part, dries in the shade.The banksia rose: record one one of Chinese Pharmacopoeia version in 2010, the 57th page, these article are the dry root of feverfew banksia rose A μ cklandia lappa decene..Autumn, two seasons of winter excavate, and remove silt and fibrous root, segment, and big vertical profile again becomes lobe, hits tertia after the drying.The myrobalan: record one one of Chinese Pharmacopoeia version in 2010, the 173rd page, these article are the dry mature fruit of combretaceae plant myrobalan Terminalia cheb μ la Retz. or fine hair myrobalan Terminalia cheb μ la Retz.var.tomentella K μ rt..Two season of autumn and winter gathers during fruit maturation, removes impurity, dries.Frankincense: record one one of Chinese Pharmacopoeia version in 2010, the 207th page, the resin that these article ooze out for olive subject plant Boswellia carterii Boswellia carterii Birdw. and congener Boswellia bhaw-dajiana Birdw. bark.Be divided into Somalia's frankincense and Ethiopia's frankincense, every kind of frankincense is divided into frankincense pearl and former frankincense again.Fructus choerospondiatis: record one one of Chinese Pharmacopoeia version in 2010, the 41st page, these article are Mongols's conventional crude drugs, are the dry mature fruit of Anacardiaceae plant south wild jujube Choerospondias axillaris (Roxb) B μ rtt et Hill.Gather during fruit maturation autumn, removes impurity, drying.Nutmeg: record one one of Chinese Pharmacopoeia version in 2010, the 126th page, these article are the drying kind benevolence of myristicaceae plant nutmeg Myristica fragrans Ho μ tt..Common bombax flower: record one one of Chinese Pharmacopoeia version in 2010, the 59th page, these article are the dried floral of Bombacaceae plant kapok Gossampin μ s malabarica (DC.) Merr..Spend spring when blooming and gather, remove impurity, dry.Tufa: record in first the 25th page of Ministry of Health of the People's Republic of China's Tibetan medicine ministerial standard (standard numbering: WS3-BC-0025-95).These article are carbonate mineral, main carbonated calcium (CaCo3).All can gather the whole year, removes earth, assorted stone.

The Bawei Chenxiang ball has the heat of clearing away heart-fire, and the peaceful heart is calmed the nerves the effect of having one's ideas straightened out.Be used for pyreticosis and make a psychological attack, coma and delirium, coronary heart disease, angina pectoris.Microscopical identification in the primary standard has just been explained diagnostic characteristics, does not point out that which kind of medicinal material is which characteristic be; Conditions such as unified method for distilling and developping agent are adopted in the discriminating of agalloch eaglewood and frankincense in the primary standard in addition, and the repeatability of standard and specificity are all very poor.

Summary of the invention

The object of the invention is to disclose that a kind of specificity is strong, the detection method of favorable reproducibility, Bawei Chenxiang preparation easy and simple to handle.

The present invention seeks to realize through following scheme.

The detection method of Bawei Chenxiang preparation, this detection method comprise one or more in following microscopical identification and the thin layer discriminating:

A, get the Bawei Chenxiang preparation and add chloral hydrate and change 2-4 time thoroughly, film-making is put microscopically and is observed, and differentiates the morphological feature of the medicinal material of respectively distinguishing the flavor of, and wherein: the main morphological feature of the banksia rose: synanthrin sees that the surface shows radial texture more; The main morphological feature of common bombax flower: the nonglandular hair fragment sees that wall is thick slightly more, and is straight or crooked slightly, contains dark granular substance in the cell that has; Myrobalan's main morphological feature: the endocarp lithocyte is similar round, ellipse, strip or irregular shape, and the prolongation that has is fibrous, or branch is arranged, wall thickness, and the hole ditch is obvious, and the cell that has includes the yellowish-brown thing;

B. the thin layer of frankincense, the banksia rose is differentiated: get Bawei Chenxiang preparation 1-5 weight portion, porphyrize adds acetone 5-30 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 1-3 parts by volume, as need testing solution; Other gets frankincense, each 0.1-2 weight portion of banksia rose control medicinal material, adds acetone 5-30 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 1-3 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; " each 0.001-0.01 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; Cyclohexane-ethyl acetate with 5-15: 0.2-2 is developping agent, and thin layer plate is put saturated 0～40min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color;

C. myrobalan's thin layer is differentiated: get Bawei Chenxiang preparation 2-6 weight portion, porphyrize adds 70% ethanol 10-50 parts by volume, and sonicated 10-45min filters, the filtrating evaporate to dryness, and residue adds 2-8 parts by volume ethanol makes dissolving, as need testing solution; Other gets myrobalan's control medicinal material 0.5-2 weight portion, adds 70% ethanol 10-50 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 2-8 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; " each 0.001-0.01 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; Methenyl choloride-acetone-formic acid with 2-15: 1-6: 0.2-2 is developping agent, and thin layer plate is put saturated 0～40min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color;

Said Bawei Chenxiang preparation raw material medicine consists of: agalloch eaglewood 80-120 weight portion, nutmeg 20-120 weight portion, fructus choerospondiatis 45-120 weight portion, weight portion, myrobalan 80-320 weight portion, frankincense 25-80 weight portion, banksia rose 80-200 weight portion, common bombax flower 45-100 weight portion, tufa 30-150 weight portion.

The discriminating of Bawei Chenxiang preparation of the present invention is preferably:

B. the thin layer of frankincense, the banksia rose is differentiated: get Bawei Chenxiang preparation 3 weight portions, porphyrize adds acetone 25 parts by volume, and sonicated 30min filters, and filtrating is concentrated into 2 parts by volume, as need testing solution; Other gets frankincense, each 0.5 weight portion of banksia rose control medicinal material, adds acetone 25 parts by volume, and sonicated 30min filters, and filtrating is concentrated into 2 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; " each 0.005 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With cyclohexane-ethyl acetate of 9: 1 was developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launched; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color;

C. myrobalan's thin layer is differentiated

Get Bawei Chenxiang preparation 4 weight portions, porphyrize adds 70% ethanol, 30 parts by volume, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 5 parts by volume ethanol makes dissolving, as need testing solution; Other gets myrobalan's control medicinal material 1 weight portion, adds 70% ethanol, 30 parts by volume, and sonicated 30min filters, and filtrating is concentrated into 5 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; " each 0.005 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With 7: 3: 1 methenyl choloride-acetone-formic acid was developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launched; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Said Bawei Chenxiang preparation raw material medicine composition is preferably: agalloch eaglewood 100 weight portions, nutmeg 100 weight portions, fructus choerospondiatis 100 weight portions, myrobalan's 100 weight portions, frankincense 50 weight portions, the banksia rose 175 weight portions, common bombax flower 75 weight portions, tufa 50 weight portions;

Said Bawei Chenxiang preparation raw material medicine composition is preferably: agalloch eaglewood 100 weight portions, nutmeg 40 weight portions, fructus choerospondiatis 65 weight portions, myrobalan's (stoning) 300 weight portions, frankincense 60 weight portions, the banksia rose 100 weight portions, common bombax flower 65 weight portions, tufa 120 weight portions;

The method for making of said Bawei Chenxiang preparation does; More than eight the flavor, be ground into fine powder, sieve, mixing adds the suitable quantity of water pill, drying in the shade promptly gets; Or get said Bawei Chenxiang preparation raw material medicine, and be ground into fine powder, sieve, mixing, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes the oral formulations of accepting clinically, like pill, powder, granule, capsule, tablet etc.

The relation of weight portion according to the invention and parts by volume is a kg/liter, grams per milliliter.

Description of drawings:

Fig. 1 differentiates the thin-layer chromatogram (put under the ultraviolet lamp 365nm and inspect) of agalloch eaglewood according to the ministerial standard discrimination method

Be followed successively by from left to right: 1～4: the sample, 5～6 of the embodiment of the invention 1 preparation: agalloch eaglewood control medicinal material, 7: agalloch eaglewood negative control: 8: frankincense control medicinal material, 9: frankincense negative control, 10～13: the sample of the embodiment of the invention 8 preparations.

Fig. 2 differentiates the thin-layer chromatogram (spraying the vanillic aldehyde sulfuric acid solution with 5%) of frankincense according to the ministerial standard discrimination method

Fig. 3 discrimination method of the present invention is differentiated the thin-layer chromatogram of frankincense, the banksia rose

Be followed successively by from left to right: 1～3: the sample, 4～5 of the embodiment of the invention 1 preparation: frankincense control medicinal material, 6: frankincense negative control, 7～9: the sample, 10～11 of the embodiment of the invention 8 preparations: banksia rose control medicinal material, 12: banksia rose negative control.

Fig. 4: discrimination method of the present invention is differentiated myrobalan's thin layer discriminating figure

Be followed successively by from left to right: 1: the sample, 2～3 of the embodiment of the invention 1 preparation: the sample, 4～5 of the embodiment of the invention 8 preparations: myrobalan's control medicinal material; 6: myrobalan's negative control.

The present invention has carried out microscopical identification to Bawei Chenxiang ball Chinese crude drug, and has spelt out which kind of medicinal material and have which characteristic; The present invention has carried out a large amount of thin layer Study on Identification to prescription medicinal material in the Bawei Chenxiang ball, and test findings shows that conditions such as unified method for distilling and developping agent are adopted in the discriminating of agalloch eaglewood and frankincense in the primary standard; Because the spot colour developing is unintelligible, degree of separation is poor, repeatability; Specificity is relatively poor, has therefore cancelled the thin-layer chromatography of agalloch eaglewood and has differentiated, on the basis of primary standard; The newly-increased banksia rose, myrobalan's specificity thin layer discrimination method, and through a large amount of thin layer discrimination test discovery frankincenses and unified method for distilling of banksia rose discriminating employing and developping agent, compare repeatability and specificity with primary standard stronger; So the frankincense in the primary standard is made an amendment, meet easy, principle fast.

Following experimental example and embodiment further specify but are not limited to the present invention.

Test Example 1: the thin layer discrimination method research of agalloch eaglewood, frankincense

1.1 differentiate agalloch eaglewood and frankincense according to the ministerial standard discrimination method

The preparation of need testing solution: get the fine powder 3g of the embodiment of the invention 1,8 preparations respectively, add acetone 15ml, sonicated 30min filters, and filtrating is concentrated into 2ml, as need testing solution 1, need testing solution 2.

The preparation of agalloch eaglewood control medicinal material solution: get each 0.18g of agalloch eaglewood control medicinal material, add acetone 15ml, sonicated 30min filters, and filtrating is concentrated into 2ml, processes agalloch eaglewood control medicinal material solution.

The preparation of frankincense control medicinal material solution: get each 0.18g of frankincense control medicinal material, add acetone 15ml, sonicated 30min filters, and filtrating is concentrated into 2ml, processes frankincense control medicinal material solution.

The preparation of agalloch eaglewood negative sample solution: in the prescription ratio, preparation does not contain the negative sample of agalloch eaglewood, and processes agalloch eaglewood negative sample solution according to the preparation method of above-mentioned need testing solution.

The preparation of frankincense negative sample solution: in the prescription ratio, preparation does not contain the negative sample of frankincense, and processes frankincense negative sample solution according to the preparation method of above-mentioned need testing solution.

Adsorbent: silica G plate (Haiyang Chemical Plant, Qingdao).

Point sample amount: each 10 μ l of need testing solution, agalloch eaglewood, frankincense control medicinal material solution and negative sample solution.

Developping agent: toluene-acetone-formic acid (8: 1: 0.2).

Developer: 5% vanillic aldehyde sulfuric acid solution (take by weighing the 1g vanillic aldehyde, add the 20ml concentrated sulphuric acid and make dissolving, promptly get).

Conclusion: the photograph thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 10 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With toluene-acetone-formic acid (8: 1: 0.2) is developping agent; Launch, take out, dry; Put under the ultraviolet lamp (365nm) and inspect, the result sees accompanying drawing 1; Spray the vanillic aldehyde sulfuric acid solution with 5% then, 90 ℃ were toasted 5 minutes, took out, and the result sees accompanying drawing 2; The spot of spot of agalloch eaglewood and colour developing back frankincense all develops the color unintelligiblely under the fluorescence, and degree of separation is poor, repeats that the result is still undesirable several times, so in this detection method, cancelled the thin layer discriminating of agalloch eaglewood.

1.2 discrimination method of the present invention is differentiated frankincense, the banksia rose

(1) instrument

Mortar, graduated cylinder, round-bottomed flask, analytical balance, Extraction by Ultrasound device, evaporating dish, sample applicator, silica gel g thin-layer plate, chromatography cylinder, spray bottle.

(2) control medicinal material

Frankincense control medicinal material, banksia rose control medicinal material.

(3) reagent

Sherwood oil (boiling range 60-90 ℃), ethyl acetate, acetone, methyl alcohol, the concentrated sulphuric acid, vanillic aldehyde, distilled water, benzene, toluene, formic acid, cyclohexane.

(4) method of inspection:

Extract choice of Solvent: adopt sherwood oil (boiling range 60-90 ℃), ethyl acetate, acetone, methyl alcohol equal solvent respectively for extracting solvent;

The selection of method for distilling: adopt ultrasonic Extraction and cold soaking respectively;

The selection of developping agent: adopt benzene-acetone-formic acid (8: 1: 0.2) respectively, toluene-acetone-formic acid (8: 1: 0.2), cyclohexane-acetone (10: 3), cyclohexane-ethyl acetate (9: 1) is developping agent.

Select above solvent, method for distilling and the developping agent of extracting to make an experiment respectively, the result is following:

Under above condition, through repetition test, confirmed that finally the specificity thin layer discrimination method of frankincense, the banksia rose is following:

The preparation of need testing solution: get the fine powder 3g of the embodiment of the invention 1,8 preparations, add acetone 25ml, sonicated 30min filters, and filtrating is concentrated into 2ml, as need testing solution 1, need testing solution 2.

The preparation of frankincense control medicinal material solution: get frankincense control medicinal material 0.5g, add acetone 25ml, sonicated 30min filters, and filtrating is concentrated into 2ml, processes frankincense control medicinal material solution.

The preparation of banksia rose control medicinal material solution: get banksia rose control medicinal material 0.5g, add acetone 25ml, sonicated 30min filters, and filtrating is concentrated into 2ml, processes banksia rose control medicinal material solution.

The preparation of banksia rose negative sample solution: in the prescription ratio, preparation does not contain the negative sample of the banksia rose, and processes banksia rose negative sample solution according to the preparation method of above-mentioned need testing solution.

Adsorbent: silica G plate (Haiyang Chemical Plant, Qingdao).

Point sample amount: need testing solution, frankincense, banksia rose control medicinal material solution and frankincense, each 5 μ l of banksia rose negative sample solution.

Developping agent: cyclohexane-ethyl acetate (9: 1).

Conclusion: according to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, drawing each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developping agent with cyclohexane-ethyl acetate (9: 1), launches, and taking-up is dried.Spray is with 5% vanillic aldehyde sulfuric acid solution, and it is clear that hot blast blows to the spot colour developing, and the result sees accompanying drawing 3.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color, the spot degree of separation is better, Rf value is moderate, and other compositions do not have obvious interference to the discriminating of frankincense, the banksia rose just.

Test Example 2: myrobalan's thin layer discrimination method research

(1) instrument

(2) control medicinal material

Myrobalan's control medicinal material.

(3) reagent

Acetone, methyl alcohol, ethanol, the concentrated sulphuric acid, vanillic aldehyde, distilled water, benzene, glacial acetic acid, toluene, formic acid, methenyl choloride, methylene chloride, 10% sulfuric acid ethanol, 10% vanillic aldehyde sulfuric acid, 10% ferric trichloride.

(4) method of inspection:

Extract choice of Solvent: difference 70% ethanol, ethanol, 70% methyl alcohol, the methyl alcohol equal solvent is for extracting solvent;

The selection of method for distilling: adopt ultrasonic Extraction and heating reflux method respectively;

The selection of developping agent: adopt benzene-glacial acetic acid-water (12: 10: 0.4) respectively, toluene-glacial acetic acid-water (12: 10: 0.4), methylene chloride-acetone-formic acid (7: 3: 1), methenyl choloride-acetone-formic acid (7: 3: 1) is developping agent;

The selection of developer: 10% sulfuric acid ethanol, 10% vanillic aldehyde sulfuric acid, 10% ferric trichloride.

Select above solvent, method for distilling, developping agent and the developer of extracting to make an experiment respectively, the result is following:

Under above condition, confirmed finally that through repetition test the specificity thin layer discrimination method of frankincense, the banksia rose is following:

The preparation of need testing solution: get the fine powder 4g of the embodiment of the invention 1,8 preparations, add 70% ethanol 30ml, sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, as need testing solution 1, need testing solution 2.

The preparation of myrobalan's control medicinal material solution: get myrobalan's control medicinal material solution 1g, process myrobalan's control medicinal material solution with method.

The preparation of myrobalan's negative sample solution: in the prescription ratio, preparation does not contain myrobalan's negative control, and processes myrobalan's negative sample solution according to the preparation method of above-mentioned need testing solution.

Adsorbent: silica G plate (Haiyang Chemical Plant, Qingdao).

Point sample amount: each 5 μ l of need testing solution, myrobalan's control medicinal material solution and myrobalan's negative sample solution.

Developping agent: methenyl choloride-acetone-formic acid (7: 3: 1).

Developer: 10% liquor ferri trichloridi.

Conclusion: according to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, drawing each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developping agent with methenyl choloride-acetone-formic acid (7: 3: 1), launches, and taking-up is dried.Spray is with 10% liquor ferri trichloridi, and it is clear that hot blast blows to the spot colour developing, and the result sees accompanying drawing 4.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color, the spot degree of separation is better, Rf value is moderate, and other compositions do not have obvious interference to myrobalan's discriminating just.

Following embodiment all can realize the effect of above-mentioned experimental example.

Embodiment

Embodiment 1: the discriminating of Bawei Chenxiang ball

Agalloch eaglewood 100g, nutmeg 100g, fructus choerospondiatis 100g, myrobalan 100g, frankincense 50g, banksia rose 175g, common bombax flower 75g, tufa 50g;

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing adds water and processes ball, and drying promptly gets.Discrimination method:

A. microscopical identification

Get above-mentioned preparation and add chloral hydrate and change thoroughly 3 times, film-making, put microscopically and observe: synanthrin sees that the surface shows radial texture more; The nonglandular hair fragment sees that wall is thick slightly more, and is straight or crooked slightly, contains dark granular substance in the cell that has; The endocarp lithocyte is similar round, ellipse, strip or irregular shape, and the prolongation that has is fibrous, or branch is arranged, wall thickness, and the hole ditch is obvious, and the cell that has includes the yellowish-brown thing.

B. the discriminating of frankincense, the banksia rose

Get the fine powder 3g of above-mentioned formulation preparation, add acetone 25ml, sonicated 30min filters, and filtrating is concentrated into 2ml, as need testing solution.Other gets frankincense, each 0.5g of banksia rose control medicinal material, adds acetone 25ml, and sonicated 30min filters, and filtrating is concentrated into 2ml, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (9: 1) is developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 4g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 30ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1g, adds 70% ethanol 30ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (7: 3: 1) is developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 2: the discriminating of Bawei Chenxiang granule

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes granule.

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 1.5g of above-mentioned formulation preparation, add acetone 10ml, sonicated 10min filters, and filtrating is concentrated into 1.5ml, as need testing solution.Other gets frankincense, each 0.2g of banksia rose control medicinal material, adds acetone 5ml, and sonicated 10min filters, and filtrating is concentrated into 1ml, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 2 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (6.8: 1) is developping agent, and thin layer plate is put saturated 15min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 2.5g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 15ml, and sonicated 15min filters, the filtrating evaporate to dryness, and residue adds 2ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 0.8g, adds 70% ethanol 10ml, and sonicated 15min filters, the filtrating evaporate to dryness, and residue adds 6ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (8: 1: 0.3) is developping agent, and thin layer plate is put saturated 15min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 3: the discriminating of Bawei Chenxiang honeyed bolus

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes honeyed bolus.

A. microscopical identification

Get above-mentioned preparation and add chloral hydrate and change thoroughly 2 times, film-making, put microscopically and observe: synanthrin sees that the surface shows radial texture more; The nonglandular hair fragment sees that wall is thick slightly more, and is straight or crooked slightly, contains dark granular substance in the cell that has; The endocarp lithocyte is similar round, ellipse, strip or irregular shape, and the prolongation that has is fibrous, or branch is arranged, wall thickness, and the hole ditch is obvious, and the cell that has includes the yellowish-brown thing.

B. the discriminating of frankincense, the banksia rose

Get the fine powder 2.5g of above-mentioned formulation preparation, add acetone 20ml, sonicated 20min filters, and filtrating is concentrated into 2ml, as need testing solution.Other gets frankincense, each 1.2g of banksia rose control medicinal material; Add acetone 15ml, sonicated 20min filters; Filtrating is concentrated into 2ml; Process control medicinal material solution by material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 4 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (13.4: 1) is developping agent, and thin layer plate is put saturated 25min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 3g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 20ml, and sonicated 10min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1g, adds 70% ethanol 20ml, and sonicated 10min filters, the filtrating evaporate to dryness, and residue adds 8ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 10 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (9.1: 3.8: 1.2) is developping agent, and thin layer plate is put saturated 25min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 4: the discriminating of Bawei Chenxiang powder

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes powder.

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 4g of above-mentioned formulation preparation, add acetone 25ml, sonicated 30min filters, and filtrating is concentrated into 2.5ml, as need testing solution.Other gets frankincense, each 0.8g of banksia rose control medicinal material; Add acetone 25ml, sonicated 30min filters; Filtrating is concentrated into 2.5ml; Process control medicinal material solution by material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (10: 1) is developping agent, and thin layer plate is put saturated 30min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 6g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 50ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 3ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 2g, adds 70% ethanol 40ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 3ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 8 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (11: 4: 0.5) is developping agent, and thin layer plate is put saturated 30min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 5: the discriminating of Bawei Chenxiang ball

Agalloch eaglewood 85g, nutmeg 25g, fructus choerospondiatis 50g, myrobalan 85g, frankincense 30g, banksia rose 85g, common bombax flower 50g, tufa 35g;

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Get the fine powder 2g of abovementioned alkyl phenol polyethenoxy formulation preparation, porphyrize adds 70% ethanol 10ml, and sonicated 20min filters, the filtrating evaporate to dryness, and residue adds 4ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 0.6g, adds 70% ethanol 10ml, and sonicated 20min filters, the filtrating evaporate to dryness, and residue adds 4ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 4 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (3: 1.2: 0.4) is developping agent, and thin layer plate is put saturated 10min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 6: the discriminating of Bawei Chenxiang capsule agent

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes capsule.

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 4.5g of above-mentioned formulation preparation, add acetone 30ml, sonicated 45min filters, and filtrating is concentrated into 2ml, as need testing solution.Other gets frankincense, each 1.5g of banksia rose control medicinal material; Add acetone 20ml, sonicated 45min filters; Filtrating is concentrated into 2.5ml; Process control medicinal material solution by material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 1.5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (11: 1) is developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Embodiment 7: the discriminating of Bawei Chenxiang tablet

Get above eight flavor bulk drugs according to above-mentioned composition, be ground into fine powder, sieve, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes tablet.

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 2g of above-mentioned formulation preparation, add acetone 15ml, sonicated 25min filters, and filtrating is concentrated into 2ml, as need testing solution.Other gets frankincense, each 0.5g of banksia rose control medicinal material, adds acetone 15ml, and sonicated 25min filters, and filtrating is concentrated into 2ml, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 8 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (12: 1) is developping agent, and thin layer plate is put saturated 10min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 2g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 10ml, and sonicated 20min filters, the filtrating evaporate to dryness, and residue adds 4ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 0.6g, adds 70% ethanol 10ml, and sonicated 20min filters, the filtrating evaporate to dryness, and residue adds 4ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 4 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (3: 1.2: 0.4) is developping agent, and thin layer plate is put saturated 10min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 8: the discriminating of Bawei Chenxiang ball

Agalloch eaglewood 100g, nutmeg 40g, fructus choerospondiatis 65g, myrobalan 300g, frankincense 60g, banksia rose 100g, common bombax flower 65g, tufa 120g;

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Embodiment 9: the discriminating of Bawei Chenxiang capsule agent

Agalloch eaglewood 115g, nutmeg 115g, fructus choerospondiatis 115g, myrobalan 200g, frankincense 75g, banksia rose 190g, common bombax flower 95g, tufa 145g;

A. the discriminating of frankincense, the banksia rose

B. myrobalan's discriminating

Embodiment 10: the discriminating of Bawei Chenxiang granule

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Get the fine powder 3.5g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 30ml, and sonicated 25min filters, the filtrating evaporate to dryness, and residue adds 6ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1.5g, adds 70% ethanol 25ml, and sonicated 25min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 2 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (13.2: 5: 1.5) is developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 11: the discriminating of Bawei Chenxiang honeyed bolus

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 1g of above-mentioned formulation preparation, add acetone 8ml, sonicated 15min filters, and filtrating is concentrated into 1ml, as need testing solution.Other gets frankincense, each 1g of banksia rose control medicinal material; Add acetone 10ml, sonicated 15min filters; Filtrating is concentrated into 1.5ml; Process control medicinal material solution by material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 10 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (8.7: 1) is developping agent, and thin layer plate is put saturated 25min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Embodiment 12: the discriminating of Bawei Chenxiang tablet

Agalloch eaglewood 90g, nutmeg 110g, fructus choerospondiatis 80g, myrobalan 280g, frankincense 40g, banksia rose 140g, common bombax flower 80g, tufa 90g;

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Embodiment 13: the discriminating of Bawei Chenxiang powder

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 4g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 25ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 2.5ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 0.8g, adds 70% ethanol 25ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 2.5ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (10: 1) is developping agent, and thin layer plate is put saturated 30min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 4.5g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 40ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 8ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1.2g, adds 70% ethanol 35ml, and sonicated 30min filters, the filtrating evaporate to dryness, and residue adds 4ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 5 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (14.5: 5.4: 1.8) is developping agent, and thin layer plate is put saturated 30min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 14: the discriminating of Bawei Chenxiang capsule agent

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

Get the fine powder 5g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 30ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 3ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 2g, adds 70% ethanol 30ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 3ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 10 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With cyclohexane-ethyl acetate (10.5: 1) is developping agent, and thin layer plate is put saturated 15min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

C. myrobalan's discriminating

Get the fine powder 6g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 50ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 7ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1.5g, adds 70% ethanol 40ml, and sonicated 40min filters, the filtrating evaporate to dryness, and residue adds 7ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 8 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (11: 4: 0.5) is developping agent, and thin layer plate is put saturated 30min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 15: the discriminating of Bawei Chenxiang tablet

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Get the fine powder 5g of above-mentioned formulation preparation, porphyrize adds 70% ethanol 45ml, and sonicated 45min filters, the filtrating evaporate to dryness, and residue adds 5ml ethanol makes dissolving, as need testing solution.Other gets myrobalan's control medicinal material 1.8g, adds 70% ethanol 45ml, and sonicated 45min filters, the filtrating evaporate to dryness, and residue adds 8ml ethanol makes dissolving, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia version in 2010 B) test, draw each 6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate; With methenyl choloride-acetone-formic acid (4: 2: 0.6) is developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

Embodiment 16: the discriminating of Bawei Chenxiang granule

Agalloch eaglewood 110g, nutmeg 70g, fructus choerospondiatis 90g, myrobalan 150g, frankincense 55g, banksia rose 140g, common bombax flower 70g, tufa 80g;

Discrimination method:

A. microscopical identification

B. the discriminating of frankincense, the banksia rose

C. myrobalan's discriminating

Claims

1. the detection method of a Bawei Chenxiang preparation is characterized in that this detection method identifies frankincense, the banksia rose in the said Bawei Chenxiang preparation through the thin-layer chromatography discrimination method;

Get Bawei Chenxiang preparation 1-5 weight portion, porphyrize adds acetone 5-30 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 1-3 parts by volume, as need testing solution; Other gets frankincense, each 0.1-2 weight portion of banksia rose control medicinal material, adds acetone 5-30 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 1-3 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; " each 0.001-0.01 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; Cyclohexane-ethyl acetate with 5-15: 0.2-2 is developping agent, and thin layer plate is put saturated 0～40min in the expansion cylinder, launches; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

2. the detection method of a Bawei Chenxiang preparation is characterized in that this detection method identifies said Bawei Chenxiang preparation myrobalan through the thin-layer chromatography discrimination method;

Get Bawei Chenxiang preparation 2-6 weight portion, porphyrize adds 70% ethanol 10-50 parts by volume, and sonicated 10-45min filters, the filtrating evaporate to dryness, and residue adds 2-8 parts by volume ethanol makes dissolving, as need testing solution; Other gets myrobalan's control medicinal material 0.5-2 weight portion, adds 70% ethanol 10-50 parts by volume, and sonicated 10-45min filters, and filtrating is concentrated into the 2-8 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain myrobalan's negative sample, and processes myrobalan's negative sample solution by the preparation method of said need testing solution; " each 0.001-0.01 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; Methenyl choloride-acetone-formic acid with 2-15: 1-6: 0.2-2 is developping agent, and thin layer plate is put saturated 0～40min in the expansion cylinder, launches; Take out; Dry, spray the liquor ferri trichloridi with 10%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

3. according to claim 1 or claim 2 detection method is characterized in that wherein said Bawei Chenxiang preparation raw material medicine consists of: agalloch eaglewood 80-120 weight portion, nutmeg 20-120 weight portion, fructus choerospondiatis 45-120 weight portion, weight portion, myrobalan 80-320 weight portion, frankincense 25-80 weight portion, banksia rose 80-200 weight portion, common bombax flower 45-100 weight portion, tufa 30-150 weight portion.

4. detection method as claimed in claim 1 is characterized in that frankincense wherein, the discriminating of the banksia rose are:

Get Bawei Chenxiang preparation 3 weight portions, porphyrize adds acetone 25 parts by volume, and sonicated 30min filters, and filtrating is concentrated into 2 parts by volume, as need testing solution; Other gets frankincense, each 0.5 weight portion of banksia rose control medicinal material, adds acetone 25 parts by volume, and sonicated 30min filters, and filtrating is concentrated into 2 parts by volume, processes control medicinal material solution; Press material rate and method for making, configuration does not contain the negative sample of frankincense, the banksia rose, and processes frankincense, banksia rose negative sample solution by the preparation method of said need testing solution; " each 0.005 parts by volume of above-mentioned solution is drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, puts respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With cyclohexane-ethyl acetate of 9: 1 was developping agent, and thin layer plate is put saturated 20min in the expansion cylinder, launched; Take out; Dry, spray the vanillic aldehyde sulfuric acid solution with 5%, it is clear that hot blast blows to the spot colour developing; In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the spot of same color.

5. detection method as claimed in claim 2 is characterized in that myrobalan's wherein discriminating is:

6. like the arbitrary described detection method of claim 1-5, it is characterized in that wherein said Bawei Chenxiang preparation raw material medicine consists of: agalloch eaglewood 100 weight portions, nutmeg 100 weight portions, fructus choerospondiatis 100 weight portions, myrobalan's 100 weight portions, frankincense 50 weight portions, the banksia rose 175 weight portions, common bombax flower 75 weight portions, tufa 50 weight portions.

7. like the arbitrary described detection method of claim 1-5, it is characterized in that wherein said Bawei Chenxiang preparation raw material medicine consists of: agalloch eaglewood 100 weight portions, nutmeg 40 weight portions, fructus choerospondiatis 65 weight portions, myrobalan's (stoning) 300 weight portions, frankincense 60 weight portions, the banksia rose 100 weight portions, common bombax flower 65 weight portions, tufa 120 weight portions.

8. like the arbitrary described detection method of claim 1-5, it is characterized in that wherein said Bawei Chenxiang preparation processed by following method; More than eight the flavor, be ground into fine powder, sieve, mixing adds the suitable quantity of water pill, drying in the shade promptly gets; Or get said Bawei Chenxiang preparation raw material medicine, and be ground into fine powder, sieve, mixing, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes the oral formulations of accepting clinically.

9. detection method as claimed in claim 6 is characterized in that wherein said Bawei Chenxiang preparation processed by following method; More than eight the flavor, be ground into fine powder, sieve, mixing adds the suitable quantity of water pill, drying in the shade promptly gets; Or get said Bawei Chenxiang preparation raw material medicine, and be ground into fine powder, sieve, mixing, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes the oral formulations of accepting clinically.

10. detection method as claimed in claim 7 is characterized in that wherein said Bawei Chenxiang preparation processed by following method; More than eight the flavor, be ground into fine powder, sieve, mixing adds the suitable quantity of water pill, drying in the shade promptly gets; Or get said Bawei Chenxiang preparation raw material medicine, and be ground into fine powder, sieve, mixing, mixing by the pharmacy conventional method, adds conventional auxiliary material, processes the oral formulations of accepting clinically.

11., it is characterized in that wherein said oral formulations is pill, powder, granule, capsule or tablet like claim 9 or 10 described detection methods.

12. detection method as claimed in claim 8 is characterized in that wherein said oral formulations is pill, powder, granule, capsule or tablet.