CN103820641A - Fungus pre-oxidization method for arsenic-containing and carbon-containing double refractorygoldore - Google Patents
Fungus pre-oxidization method for arsenic-containing and carbon-containing double refractorygoldore Download PDFInfo
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Abstract
The invention aims at providing a method for effectively treating arsenic-containing sulfides and gold absorption carbonaceous materials in gold ore, which is urgently needed for overcoming the defects of long flow, complex process, high production cost and the like for treating double refractorygoldore by a two-step biological oxidation method. The invention provides a fungus pre-oxidization method for arsenic-containing and carbon-containing double refractorygoldore. The fungus pre-oxidization method comprises the following steps: carrying out amplification culture by using Phanerochaete chrysporium-CCTCC M2013616; then adding a nitrogen-limited medium, gold ore powder and fungus solution into a biological oxidation constant-temperature mixer; carrying out non-sterilizing oxidative degradation on the mixture for 5-15 days to enable the contents of arsenic and carbon in the gold ore powder to be greatly reduced. The gold extraction is performed on the pretreated fungus oxide slag by using a conventional cyanideprocess, so that the cyanide gold extraction rate of the gold ore powder is increased from about 10 percent to over 89 percent, one-step oxidation pretreatment of the arsenic-containing and carbon-containing double refractorygoldore is realized and remarkable economic benefit is obtained.
Description
Technical field
The invention belongs to biological metallurgy technical field, particularly the microbe-preoxidation gold method of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing.
Background technology
The dual difficult-treating gold mine of arsenic bearing-carbon bearing refers to and contains containing pozzuolite compound and rob golden Carbonaceous matter, the class gold mine that gold recovery is very low, such gold mine be distributed in China, the U.S., Australia and Russian etc. all over the world.In the dual difficult-treating gold mine of arsenic bearing-carbon bearing, gold often is contaminated in the sulfide minerals such as mispickel, pyrite, chalcopyrite with the form of micro--inferior micro-even Lattice Gold, thus affected golden with soak the effective of gold reagent and contact.In difficult-treating gold mine, impact is soaked golden Carbonaceous matter and is mainly comprised elemental carbon, organic acid, hydrocarbons.Wherein elemental carbon and organic acid are the golden persons of most important misfortune, and hydrocarbons and golden effect are fainter.Carbonaceous matter can be seized the gold of cyanidation by force by suction type in the process of cyanide gold-leaching, forms so-called " robbing gold " phenomenon.Due to the dual interference containing pozzuolite compound and Carbonaceous matter, the direct cyanidation gold-extracted rate of the dual difficult-treating gold mine of arsenic bearing-carbon bearing is very low, thus before cyaniding, to carry out oxidation pre-treatment, to improve golden leaching yield.The pretreatment process adopting at present mainly contains: high-temperature roasting method, biological oxidation process, pressure oxidation method etc.
High-temperature roasting method technical maturity, strong adaptability, technology are reliable, are the reliable methods that decomposes sulfide and Carbonaceous matter, raising gold leaching rate.But this technique has the following disadvantages: 1. energy consumption high, easily cause environmental pollution; 2. cross roasting and easily form golden secondary physical parcel and the crack closure of hole, thereby cause golden secondary difficulty to soak; 3. Carbonaceous matter incineration condition harshness, it is improper to control, and easily makes the residual activation of Carbonaceous matter; 4. Jin Yi and arsenic form arsenic au-alloy and vapor away.Though pressure oxidation method have gold leaching rate high, react fast, adaptable advantage, its to equipment and materials require high, technical process is complicated, initial cost is large.Research discovery, pressure oxidation method is very micro-to the higher height misfortune Gold Ore effect of organic carbon content, can activate even in some cases Carbonaceous matter.Biological oxidation process because thering is mild condition, the advantage such as flow process is simple, energy consumption is low, selective oxidation and environmental friendliness become current research focus.
Research is found, have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans), the iron sulfur-oxidizing bacterias such as Acidithiobacillus thiooxidans (Acidithiobacillus thiooxidans) and iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans) have good oxidative degradation to the mispickel in gold mine, pyrite and chalcopyrite sulfides, but the effect aspect reduction Carbonaceous matter misfortune gold of these bacteriums is not obvious.Two step biological oxidation processs, because effectively making up traditional mineral chemoautotrophic bacteria in the deficiency aspect processing Carbonaceous matter, have caused common concern.
Brierley and Kulpa first use the sulfide in the dual difficult ore of thiobacillus ferrooxidant (Thiobacillus ferrooxidans) oxidation sulfur-bearing carbon containing, then use the passivating carbonaceous thing of heterotrophism flora.Due to the degraded of sulfide and the passivation of Carbonaceous matter, the cyanidation gold-extracted rate of this gold mine brings up to 40～60% by 0%.But this technique need to add the EDTA sequestrant of high dosage, to promote to rob the passivation of golden Carbonaceous matter, and the membership that adds of high dosage EDTA causes increasing substantially of production cost.
The use rainbow conk (Trametes versicolor) such as Yen W T are carried out oxidation pre-treatment to difficult and dual difficult-treating gold mine, and the first step is to rob golden Carbonaceous matter with the substratum passivation of carrying disease germs, and the leaching yield of gold is 54.1～64.5%.Second step is to use the not substratum sulfide oxidation of carrier, after two steps are processed, puies forward golden rate up to 87.0～95.25%.The key constraints of this patent is the removal that the first step finishes rear thalline, and in degraded system, continues maintaining of degradation condition after thalline removal.
The use two step bacterial oxidation methods such as Amankwa R K are destroyed dual golden ore concentrate hard to treat, the first step is with mineral chemoautotrophic bacteria oxidation of sulfureted mineral, cyanidation gold-extracted rate is 81.1%, second step is to decompose Carbonaceous matter with crinosity streptomycete (Streptomyces setonii), after Streptomyces setonii processes, in gold ore, the content of carbon reduces, and the combined action of above two steps makes cyanidation gold-extracted rate bring up to 94.7%.
Two step biological oxidation processs, because relating to linking and the conversion of two kinds of degraded systems, therefore have the drawbacks such as long flow path, complex process, production cost height.Because above-mentioned pretreatment process has limitation separately aspect the dual difficult-treating gold mine of processing, therefore can effectively process the method containing pozzuolite compound and the golden Carbonaceous matter of misfortune in gold mine in the urgent need to a kind of, to realize the economy of the dual difficult-treating gold mine resource of arsenic bearing-carbon bearing, efficiently to utilize.
Summary of the invention
For the problem existing in above-mentioned pretreatment process, the invention provides the fungi method for pre-oxidizing of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing.The present invention utilizes Phanerochaete chrysosporium RF-G-511 (Phanerochaete chrysosporium RF-G-511, be abbreviated as Phanerochaete chrysosporium), deposit number is CCTCC NO:M2013616, in the center preservation of Chinese Typical Representative culture collection, preservation date: on November 27th, 2013, address: China. Wuhan. Wuhan University, gold bearing sulfide in the dual difficult-treating gold mine of arsenic bearing-carbon bearing and the golden Carbonaceous matter of misfortune are carried out to oxidation pre-treatment, to open the misfortune gold of golden inclusion and reduction Carbonaceous matter, thereby improve golden leaching yield.
For achieving the above object, technical scheme of the present invention is as follows:
A fungi method for pre-oxidizing for the dual difficult-treating gold mine of arsenic bearing-carbon bearing, comprises the following steps:
(1) fungus culture
The Phanerochaete chrysosporium that is CCTCC M2013616 by deposit number is seeded in limit nitrogen substratum, and inoculum size is 3～8 × 10
5individual spore/ml, is shaking culture 3 days under 120～180rpm condition in 30～40 ℃ of culture temperature, shaking speed, and described fungi forms fine and close mycelium pellet;
(2) the dual difficult-treating gold mine of fungal oxidation arsenic bearing-carbon bearing
To limit nitrogen substratum, the fungi bacterium liquid of cultivating in gold mine powder and step (1) joins in bio-oxidation thermostatic mixer, and the volume ratio of inoculum size and substratum is 1:4～6, and gold mine pulp density (W/V) is 10～20%; This pH values of pulp is adjusted to 4.5～7.0, is that 600～1000rpm, aeration quantity are 0.1～0.3m at 30～40 ℃ of culture temperature, agitator speed
3under/h condition, carry out non-sterilizing oxidative degradation 5～15 days;
(3) separate fungal oxidation slag and cyanidation gold-extracted
Ore pulp after oxidative degradation is carried out to solid-liquid separation, the content of arsenic and carbon in detection fungal oxidation slag; Again by after fungal oxidation slag furnishing slag slurry, with CaO, NaOH, Ca (OH)
2in alkali, its pH is adjusted to 9.5～10.5; Again this oxidation sludge is proceeded to next flow process, carry out conventional cyanidation and carry gold, and calculate golden leaching yield.
The fungi method for pre-oxidizing of the dual difficult-treating gold mine of above-mentioned a kind of arsenic bearing-carbon bearing, it is improved Tien & Kirk substratum that the middle Phanerochaete chrysosporium-CCTCC M2013616 of described step (1) cultivates limit nitrogen substratum used, its composition comprises: glucose 10g/L, KH
2pO
40.2g/L, MgSO
47H
2o1.0g/L, ammonium tartrate 0.37g/L, CaCl
20.02g/L, VB
10.004g/L, micro-mixed solution 70ml/L; The composition of described micro-mixed solution comprises: glycine 0.586g/L, NaCl1.0g/L, CoSO
40.1g/L, CuSO
45H
2o0.01g/L, Na
2moO
40.01g/L, H
3bO
30.01g/L, KAl (SO
4)
20.01g/L, ZnSO
47H
2o0.1g/L, CaCl
20.082g/L, FeSO
47H
2o0.1g/L, MgSO
47H
2o3.0g/L, MnSO
47H
2o0.5g/L; Medium pH is 4.5～7.0.
The fungi method for pre-oxidizing of the dual difficult-treating gold mine of above-mentioned a kind of arsenic bearing-carbon bearing, in described step (2) the gold mine powder of granularity≤100 μ m account for whole gold mine powder ratios >=80%.
The fungi method for pre-oxidizing of the dual difficult-treating gold mine of above-mentioned a kind of arsenic bearing-carbon bearing, in described step (2), in non-sterilizing Oxidative Degradation Process, pH values of pulp remains on 4.5～7.0.
The fungi method for pre-oxidizing of the dual difficult-treating gold mine of above-mentioned a kind of arsenic bearing-carbon bearing, in described step (2), first limit nitrogen substratum and gold mine powder are joined in bio-oxidation thermostatic mixer, after high-temp steam sterilizing is processed, then the fungi bacterium liquid of cultivating in step (1) is joined in this agitator.
The present invention compared with prior art, has the following advantages:
1, the type culture that Phanerochaete chrysosporium is white-rot fungi, under the condition of Nutrition Restriction, can secrete plurality of enzymes, and these enzymes have formed Ligninolytic Enzymes system jointly, and this system mainly comprises H
2o
2produce enzyme system and enzyme system of lignin oxidation.Compared with degradation by bacteria system, Phanerochaete chrysosporium degraded system has following advantage:
(1) in Phanerochaete chrysosporium by H
2o
2producing enzyme is the H that catalysis forms
2o
2both can be used as initial oxidation substrates peroxide activator enzyme and started lignin degradation reaction, can participate in again the oxidative degradation containing pozzuolite compound.Therefore Phanerochaete chrysosporium can not only degrade and the Carbonaceous matter such as passivation coal, gac, humic acids, can also be by the Metabolic activity oxygenolysis of self containing pozzuolite compound, thus reach dearsenification decarburization and reduce Carbonaceous matter the object of robbing gold.
(2) Phanerochaete chrysosporium, without substrate for induction, only replys by Nutrition Restriction, can synthesize Ligninolytic Enzymes system, opens degradation of substrates process.The be degraded to degrading enzyme system catalysis of Phanerochaete chrysosporium to substrate take free radical as basic chain reaction process, this reaction process is followed pseudo-first-order kinetics, therefore can realize in theory the thorough degraded containing pozzuolite compound and Carbonaceous matter.
(3) Phanerochaete chrysosporium can pass through oligotrophication, produces redox system on oxyradical and plasma membrane to other microorganisms antagonistic actions, therefore can retain its competitive edge in non-sterilizing degradation process.
(4) the non-specific scope that greatly increases Phanerochaete chrysosporium degraded substrate of degraded substrate, is conducive to its removing arsenic in gold mine and carbon.
2, the present invention is contained pozzuolite compound and is robbed golden Carbonaceous matter by Phanerochaete chrysosporium simultaneous oxidation degraded, thereby can effectively overcome in dual difficult-treating gold mine two step biological oxidation processs, due to the conversion of bacterium system and heterotrophic bacterium system be connected brought series of problems, greatly simplify technical process, saved production cost.
3, Phanerochaete chrysosporium-CCTCC M2013616 is to having stronger oxidative degradation ability containing pozzuolite compound and Carbonaceous matter, after oxidation pre-treatment 5～15 days, in gold mine powder, the content of arsenic 3.51～8.16% is down to 0.28～1.12% by what start; The content of carbon is down to 0.80～1.53% by initial 2.85～3.76%.The cyanidation gold-extracted rate of gold mine powder is brought up to more than 89% by 10～30%, and economic benefit is very remarkable.
4, the antibacterial strategy of non-sterilizing degraded is cultivated in sterilizing of the present invention, can effectively solve the problem of the easy microbiological contamination of Phanerochaete chrysosporium reaction system, for the large-scale industrial application of this bacterium is laid a good foundation.
5, the present invention utilizes the dual difficult-treating gold mine of arsenic bearing-carbon bearing that Phanerochaete chrysosporium-CCTCC M2013616 cannot economical and effective utilization to other techniques to carry out oxidation pre-treatment first, for the extensive utilization of such gold ore resource provides may.
6, due to Phanerochaete chrysosporium-CCTCC M2013616 degraded system partial neutral, can effectively alleviate the acid etching harm to equipment and materials.
Accompanying drawing explanation
Fig. 1 is fungi preoxidation and the Cyanide Process schema of the dual difficult-treating gold mine of arsenic bearing-carbon bearing.
Embodiment
Cultivating Phanerochaete chrysosporium-CCTCC M2013616 limit nitrogen substratum used is improved Tien & Kirk substratum, and its composition comprises: glucose 10g/L, KH
2pO
40.2g/L, MgSO
47H
2o1.0g/L, ammonium tartrate 0.37g/L, CaCl
20.02g/L, VB
10.004g/L, micro-mixed solution 70ml/L; The composition of above-mentioned micro-mixed solution comprises: glycine 0.586g/L, NaCl1.0g/L, CoSO
40.1g/L, CuSO
45H
2o0.01g/L, Na
2moO
40.01g/L, H
3bO
30.01g/L, KAl (SO
4)
20.01g/L, ZnSO
47H
2o0.1g/L, CaCl
20.082g/L, FeSO
47H
2o0.1g/L, MgSO
47H
2o3.0g/L, MnSO
47H
2o0.5g/L; Medium pH is 4.5～7.0.
Shaking table model is: Ha Donglian constant temperature oscillator HZQ-QX.Thermostatic mixer model is: Shanghai sample model JB300-D type.
Embodiment 1
The dual golden ore concentrate hard to treat of this routine handled arsenic bearing-carbon bearing is from GOLD DEPOSIT IN SICHUAN, and the content of its principal element is in table 1, and the sreen analysis after its ore grinding shows, granularity≤100 μ m accounts for 80%, and the main gold bearing sulfide in this gold mine is pyrite and mispickel.Carry gold according to conventional cyanidation, i.e. when cyaniding, pulp density is that 25%, NaCN concentration is 0.1%, and aeration quantity is 0.2m
3/ h, stirring velocity is 1000r/min.After cyaniding 48h, filter, by 6 post-dryings of distilled water flushing, the gold content in cyanide residue and cyanating solution is measured, the direct cyanide leaching of gold is only 16.46%.
The content of table 1 example 1 gold ore principal element
1, fungus culture
Under aseptic condition, the spore inoculating that Phanerochaete chrysosporium-CCTCC M2013616 is produced is to sterilized limit nitrogen substratum, and inoculum size is 5 × 10
5individual spore/ml, is shaking culture 3 days under 120rpm condition in 30 ℃ of culture temperature, shaking speed, and described fungi forms fine and close mycelium pellet;
2, the dual difficult-treating gold mine of fungal oxidation
Limit nitrogen substratum and 300g gold mine breeze to join in bio-oxidation thermostatic mixer 2.5L, the horizontal high voltage steam sterilizing processing of going forward side by side.Then the fungi bacterium liquid 0.5L cultivating in step (1) is seeded in above-mentioned agitator, the concentration of ore pulp (W/V) is 10%, and pH values of pulp is adjusted to 4.5, is 0.2m in 30 ℃ of culture temperature, agitator speed 600rpm, aeration quantity
3non-sterilizing degraded 10 days under the condition of/h, pH values of pulp remains on 4.5～7.0;
3, separate fungal oxidation slag and cyanidation gold-extracted
Ore pulp in bio-oxidation thermostatic mixer is carried out to solid-liquid separation, and the arsenic in fungal oxidation slag and the content of carbon are respectively 0.86% and 1.25%.By after fungal oxidation slag furnishing slag slurry, pH is adjusted to 9.5 with CaO, fungal oxidation slag is proceeded to next flow process, carry out conventional cyanidation and carry gold.When cyaniding, pulp density is that 25%, NaCN concentration is 0.1%, and aeration quantity is 0.2m
3/ h, stirring velocity is 1000r/min.After cyaniding 48h, filter, by 6 post-dryings of distilled water flushing, the gold content in cyanide residue and cyanating solution is measured, and calculated golden leaching yield.After Phanerochaete chrysosporium-CCTCC M2013616 oxidation pre-treatment, the golden extraction yield of this gold ore rises to 92.37%(in table 2 by 16.46%).
Cyanidation gold-extracted result before and after the 1 gold ore bacterium effect of table 2 example
Embodiment 2
This routine handled gold ore is from Shaanxi gold mine, and the content of its principal element is in table 3, and the sreen analysis after its ore grinding shows, granularity≤100 μ m accounts for 88%.Main gold bearing sulfide in this gold mine is pyrite and mispickel, cyanidation gold-extracted according to ordinary method in embodiment 1, and the direct cyanide leaching of gold is only 12.19%.
The content of table 3 example 2 gold ore principal elements
1, fungus culture
Under aseptic condition, the spore inoculating that Phanerochaete chrysosporium-CCTCC M2013616 is produced is to sterilized limit nitrogen substratum, and inoculum size is 3 × 10
5individual spore/ml, shaking culture 3 days under 35 ℃ of culture temperature, shaking speed 150rpm condition, described fungi forms fine and close mycelium pellet;
2, the dual golden ore concentrate hard to treat of fungal oxidation
Limit nitrogen substratum and 625g gold mine breeze to join in bio-oxidation thermostatic mixer 2.5L, the horizontal high voltage steam sterilizing processing of going forward side by side.Then the fungi bacterium liquid 0.625L cultivating in step (1) is seeded in above-mentioned agitator, pulp density (W/V) is 20%, regulates pH values of pulp to 5.5, is 0.1m in 35 ℃ of culture temperature, 800rpm, aeration quantity
3non-sterilizing degraded 5 days under the condition of/h, pH values of pulp remains on 4.5～7.0;
3, separate fungal oxidation slag and cyanidation gold-extracted
Ore pulp in bio-oxidation thermostatic mixer is carried out to solid-liquid separation, and the arsenic in fungal oxidation slag and the content of carbon are respectively 0.28% and 0.80%.By after fungal oxidation slag furnishing slag slurry, adjust pH to 10 with NaOH, fungal oxidation slag is proceeded to next flow process, to carry out conventional cyanidation and carry gold, step is with embodiment 1, and calculates golden leaching yield.After Phanerochaete chrysosporium-CCTCC M2013616 oxidation pre-treatment, the golden extraction yield of this gold ore rises to 89.64%(in table 4 by 12.19%).
Cyanidation gold-extracted result before and after the 2 gold ore bacterium effects of table 4 example
Embodiment 3
This routine handled gold ore is from gold mine in Guangxi, the content of its principal element is in table 5, sreen analysis after its ore grinding shows, granularity≤100 μ m accounts for 85%, main gold bearing sulfide in this gold mine is pyrite and mispickel, cyanidation gold-extracted according to ordinary method in embodiment 1, the direct cyanide leaching of gold is only 25.38%.
The content of table 5 example 3 gold ore principal elements
1, fungus culture
Under aseptic condition, the spore inoculating that Phanerochaete chrysosporium-CCTCC M2013616 is produced is to sterilized limit nitrogen substratum, and inoculum size is 8 × 10
5individual spore/ml, shaking culture 3 days under the condition of 40 ℃ of culture temperature, shaking speed 180rpm, described fungi forms fine and close mycelium pellet;
2, the dual golden ore concentrate hard to treat of fungal oxidation
Limit nitrogen substratum and 472.5g gold mine breeze to join in bio-oxidation thermostatic mixer 2.7L, the horizontal high voltage steam sterilizing processing of going forward side by side, then the fungi bacterium liquid 0.45L cultivating in step (1) is seeded in above-mentioned agitator, pulp density is (W/V) 15%, regulating pH values of pulp to 7.0, is 0.3m in 40 ℃ of culture temperature, agitator speed 1000rpm, aeration quantity
3non-sterilizing degraded 15 days under/h condition, pH values of pulp remains on 4.5～7.0;
3, separate fungal oxidation slag and cyanidation gold-extracted
Ore pulp in bio-oxidation thermostatic mixer is carried out to solid-liquid separation, and the arsenic in fungal oxidation slag and the content of carbon are respectively 1.12% and 1.53%.By after fungal oxidation slag furnishing slag slurry, with Ca (OH)
2adjust pH to 10.5, fungal oxidation slag is proceeded to next flow process, carry out conventional cyanidation and carry gold, step is with embodiment 1, and calculates golden leaching yield.After Phanerochaete chrysosporium-CCTCC M2013616 oxidation pre-treatment, the golden extraction yield of this gold ore rises to 90.65%(in table 6 by 25.38%).
Cyanidation gold-extracted result before and after the 3 gold ore bacterium effects of table 6 example
Claims (5)
1. a fungi method for pre-oxidizing for the dual difficult-treating gold mine of arsenic bearing-carbon bearing, is characterized in that, comprises the following steps:
(1) fungus culture
The Phanerochaete chrysosporium that is CCTCC M2013616 by deposit number is seeded in limit nitrogen substratum, and inoculum size is 3～8 × 10
5individual spore/ml, is shaking culture 3 days under 120～180rpm condition in 30～40 ℃ of culture temperature, shaking speed, and described fungi forms fine and close mycelium pellet;
(2) the dual difficult-treating gold mine of fungal oxidation arsenic bearing-carbon bearing
To limit nitrogen substratum, the fungi bacterium liquid of cultivating in gold mine powder and step (1) joins in bio-oxidation thermostatic mixer, and the volume ratio of inoculum size and substratum is 1:4～6, and gold mine pulp density (W/V) is 10～20%; This pH values of pulp is adjusted to 4.5～7.0, is that 600～1000rpm, aeration quantity are 0.1～0.3m at 30～40 ℃ of culture temperature, agitator speed
3under/h condition, carry out non-sterilizing oxidative degradation 5～15 days;
(3) separate fungal oxidation slag and cyanidation gold-extracted
Ore pulp through oxidative degradation is carried out to solid-liquid separation, the arsenic in detection fungal oxidation slag and the content of carbon; Again by after fungal oxidation slag furnishing slag slurry, with CaO, NaOH, Ca (OH)
2in alkali, its pH is adjusted to 9.5～10.5; Again this oxidation sludge is proceeded to next flow process, carry out conventional cyanidation and carry gold, and calculate golden leaching yield.
2. the fungi method for pre-oxidizing of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing according to claim 1, it is characterized in that, it is improved Tien & Kirk substratum that described Phanerochaete chrysosporium-CCTCC M2013616 cultivates limit nitrogen substratum used, its composition comprises: glucose 10g/L, KH
2pO
40.2g/L, MgSO
47H
2o1.0g/L, ammonium tartrate 0.37g/L, CaCl
20.02g/L, VB
10.004g/L, micro-mixed solution 70ml/L; The composition of described micro-mixed solution comprises: glycine 0.586g/L, NaCl1.0g/L, CoSO
40.1g/L, CuSO
45H
2o0.01g/L, Na
2moO
40.01g/L, H
3bO
30.01g/L, KAl (SO
4)
20.01g/L, ZnSO
47H
2o0.1g/L, CaCl
20.082g/L, FeSO
47H
2o0.1g/L, MgSO
47H
2o3.0g/L, MnSO
47H
2o0.5g/L; Medium pH is 4.5～7.0.
3. the fungi method for pre-oxidizing of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing according to claim 1, is characterized in that, in described step (2), the gold mine powder of granularity≤100 μ m accounts for whole gold mine powder ratio >=80%.
4. the fungi method for pre-oxidizing of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing according to claim 1, is characterized in that, in described step (2), in non-sterilizing Oxidative Degradation Process, pH values of pulp remains on 4.5～7.0.
5. the fungi method for pre-oxidizing of the dual difficult-treating gold mine of a kind of arsenic bearing-carbon bearing according to claim 1, it is characterized in that, in described step (2), first limit nitrogen substratum and gold mine powder are joined in bio-oxidation thermostatic mixer, after high-temp steam sterilizing is processed, again the fungi bacterium liquid of cultivating in step (1) is joined in this agitator, carry out non-sterilizing oxidative degradation.
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| CN107641713A (en) * | 2017-09-30 | 2018-01-30 | 新疆大学 | A kind of agitator of biological oxidation pretreatment method for golden ore concentrate hard to treat |
| CN109943723A (en) * | 2019-04-03 | 2019-06-28 | 上海第二工业大学 | A method of utilizing copper and gold in Phanerochaete chrysosporium recycling electron wastes |
| CN113308605A (en) * | 2021-05-19 | 2021-08-27 | 上海第二工业大学 | Method for strengthening leaching of copper and gold in waste circuit board by phanerochaete chrysosporium by using micro-electric field |
| CN116199320A (en) * | 2023-02-16 | 2023-06-02 | 东北大学 | Optimized seed crystal for high-acid protein-containing arsenic-containing wastewater and method for stabilizing arsenic by optimized seed crystal |
| CN116199320B (en) * | 2023-02-16 | 2024-05-28 | 东北大学 | Optimized seed crystal for high-acid protein-containing arsenic-containing wastewater and method for stabilizing arsenic by optimized seed crystal |
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