CN103816277A

CN103816277A - Composition for resisting osteoporosis

Info

Publication number: CN103816277A
Application number: CN201210469066.6A
Authority: CN
Inventors: 邢秋苓
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-11-16
Filing date: 2012-11-16
Publication date: 2014-05-28

Abstract

The invention provides a composition for resisting osteoporosis. The composition comprises the following components in parts by weight: 2-10 parts of flowers carthami, 3-10 parts of astragalus membranaceus, 1-10 parts of radix puerariae, 3-8 parts of rehmannia glutinosa, and 4-7 parts of eucommia ulmoides. The composition has a good effect of inhibiting the formation of osteoclast, and is from natural medicines to achieve the effects of few side effects and safety in use. The composition has the advantages of scientific and reasonable formula, rich component resources, simple extraction method and easy industrial production.

Description

A kind of compositions of osteoporosis disease

Technical field

The present invention relates to pharmaceutical composition, especially relate to the pharmaceutical composition of osteoporosis disease.

Background technology

Along with world population aging becomes clear day by day, the sickness rate of osteoporosis raises year by year, has directly been affected the life quality of middle-aged and elderly people by the osteodynia due to osteoporosis and fracture, has become severe social problem.Human skeleton, by continuous osteogenesis and bone resorption, is being transformed always, and this transformation is transformed in bone.Keeping good balance in osteogenesis at an early age and bone resorption, but with advancing age, because this balance of a variety of causes is tended to bone resorption, the words that continue for a long time this state can make osseous tissue become fragile, and cause occurring the various osteopathias such as osteoporosis, fracture.

Medicine and the method for having developed at present multiple prevention and treatment osteoporosis, but high owing to having price, long-term taking causes multiple side effect, thereby has certain limitation on using.Compared with synthetic new drug, natural medicinal ingredients has safety, inexpensive advantage.

Summary of the invention

The object of the present invention is to provide a kind of natural drug that derives from, there is the pharmaceutical composition of the bone loss diseases such as osteoporosis disease.

In human body, osteoclast is the unique cell that has bone resorption.Research shows, when osteoclast formation becomes Showed Very Brisk, will cause the various osteopathias such as the above-mentioned osteoporosis (osteoporosis) causing due to bone loss.Therefore, suppress the effective way that osteoclast is the bone loss diseases such as treatment osteoporosis.

Applicant of the present invention is chosen at the Chinese crude drug in Chinese Chinese medicine with effects such as bone and muscle strengthening, science, and reasonable compatibility, and the experiment of the impact on osteoclast formation in conjunction with extract and component, obtain anti-osteoporotic composition of the present invention.

Anti-osteoporotic composition of the present invention, comprises following component by weight:

Flos Carthami 2-10, Radix Astragali 3-10, Radix Puerariae 1-10, Radix Rehmanniae Preparata 3-8, Cortex Eucommiae 4-7.

In preferred formula, comprise by weight following component: Flos Carthami 5, the Radix Astragali 3, Radix Puerariae 7, Radix Rehmanniae Preparata 6, the Cortex Eucommiae 5.

In more preferred formula, comprise by weight following component: Flos Carthami 8, the Radix Astragali 5, Radix Puerariae 7, Radix Rehmanniae Preparata 4, the Cortex Eucommiae 5.

The preparation method of anti-osteoporotic composition of the present invention is as follows:

1. take by weight Flos Carthami, the Radix Astragali, Radix Puerariae, Radix Rehmanniae Preparata and the Cortex Eucommiae, clean, be ground into the fritter of 1-10mm, for subsequent use;

2. co-grinding is good Flos Carthami, the Radix Astragali and Radix Puerariae, the alcohol reflux of use 40-80% 1-3 time, extracting solution cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;

3. ethanol extract is added to distilled water thermosol, then use organic solvent extraction, decompression and solvent recovery, obtains extractum I;

4. the dissolve with ethanol of extractum I doubly being measured with 2-10, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains extractum II;

6. by the lyophilization of extractum II, obtain powdered ingredients I;

7. by good co-grinding Radix Rehmanniae Preparata and the Cortex Eucommiae, soak, add 3-10 times of pure water to decoct 3 times, extracting liquid filtering, merging filtrate; The 80-95% ethanol that above-mentioned filtrate is added to 1-5 times of volume, stirs, and cold preservation is spent the night, and filters, and filtrate recycling ethanol obtains extracting solution a;

8. extracting solution a lyophilization, obtains powdered ingredients II;

9. components I and compositionⅱ are mixed, at 30-50 ℃ of dry 3-10 hour, subpackage.

In preferred extracting method, step 3. described organic solvent be the alcohol that contains 1-6 carbon atom, alkane or esters.

In preferred extracting method, at the step alcoholic solution that 4. described ethanol is 30-100%.

Compositions of the present invention has the effect of good inhibition osteoclast formation, and derives from natural drug, and few side effects has and uses safe function.

Compositions of the present invention, scientific formulation, rationally, the aboundresources of component, extracting method be simple, be easy to suitability for industrialized production.

The specific embodiment

Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.

Embodiment 1

Anti-osteoporotic composition, filling a prescription is:

Flos Carthami 8g, Radix Astragali 5g, Radix Puerariae 7g, Radix Rehmanniae Preparata 4g, Cortex Eucommiae 5g.

The extracting method of said composition is:

1. take Flos Carthami, the Radix Astragali, Radix Puerariae, Radix Rehmanniae Preparata and the Cortex Eucommiae by above-mentioned formula, clean, be ground into the fritter of 1-10mm, for subsequent use;

2. co-grinding is good Flos Carthami, the Radix Astragali and Radix Puerariae, the alcohol reflux with 75% 2 times, each 1.5 hours, after extracting solution merges, cold preservation was spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;

3. ethanol extract is added to 5 times of amount distilled water thermosols, be then extracted with ethyl acetate, decompression and solvent recovery, obtains extractum I;

4. the dissolve with ethanol by 5 times of amounts by extractum I, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains extractum II;

6. by the lyophilization of extractum II, obtain powdered ingredients I;

7. by good co-grinding Radix Rehmanniae Preparata and the Cortex Eucommiae, soak, add 5 times of pure water to decoct 3 times, each 1 hour, after merging, filters extracting solution, and filtrate adds 85% ethanol of 3 times of volumes, stirs, and cold preservation is spent the night, and filters, and filtrate recycling ethanol obtains extracting solution a;

8. extracting solution a lyophilization, obtains powdered ingredients II;

9. components I and compositionⅱ are mixed, be dried 5 hours at 35 ℃, subpackage.

Embodiment 2

The compositions that adopts embodiment 1 to obtain, measures its impact on osteoclast formation.

The formation system of osteoclast: adopt RAW264.7 cell under RANKL exists, cultivate the osteoclast that just can be formed as multinuclear for 3-5 days in α-MEM culture fluid.Tartrate resistant acid phosphatase for osteoclast (TRAP) staining forming is observed the positive apocyte of TRAP, and several 3 positive apocytes of above nuclear TRAP under the microscope, as the index of osteoclast formation.

Described RAW264.7 is the strain of cell mouse Monocytes/Macrophages, can be formed as the osteoclast of bone resorption ability under the existence of the osteoclast formation factor (RANKL).

Anti-osteoporotic composition is dissolved in α-MEM culture fluid, be mixed with finite concentration, then join in the osteoclast formation system of RAW264.7 cell, in 5% carbon dioxide, the cell culture incubator of 37 ℃, cultivate 3 days, TRAP staining dyeing for osteoclast, and statistics has 3 positive osteoclasts of above nuclear TRAP under the microscope, the results are shown in Table 1.

The impact of table 1. anti-osteoporotic composition on osteoclast formation

In the test of table 1: RAW264.7 cell is with 5 × 10 ³the concentration of/well is inoculated in 96 orifice plates, in the α-MEM culture fluid that is 10ng/ml, cultivates 3 in RANKL concentration.

In table, the concentration of anti-osteoporotic composition is the ultimate density in cell culture fluid, and each concentration is established 3 holes, and osteoclast number is the average in 3 holes.

In table, matched group is the solvent using in the time that anti-osteoporotic composition concentration is 10ug/ml, as solvent control.

In table, suppression ratio is the suppression ratio of anti-osteoporotic composition to osteoclast formation calculating with respect to matched group.

The result of table 1 is known, and anti-osteoporotic composition significantly suppresses the formation of osteoclast, along with its suppression ratio of increase of concentration also increases.

Claims

1. an anti-osteoporotic composition, is characterized in that comprising by weight following component: Flos Carthami 2-10, Radix Astragali 3-10, Radix Puerariae 1-10, Radix Rehmanniae Preparata 3-8, Cortex Eucommiae 4-7.

2. an anti-osteoporotic composition, is characterized in that comprising by weight following component: Flos Carthami 5, the Radix Astragali 3, Radix Puerariae 7, Radix Rehmanniae Preparata 6, the Cortex Eucommiae 5.

3. an anti-osteoporotic composition, is characterized in that comprising by weight following component: Flos Carthami 8, the Radix Astragali 5, Radix Puerariae 7, Radix Rehmanniae Preparata 4, the Cortex Eucommiae 5.