CN103814653B - A kind of method being differentiated seed vitality by oxygen gas flow rate - Google Patents
A kind of method being differentiated seed vitality by oxygen gas flow rate Download PDFInfo
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- CN103814653B CN103814653B CN201210462127.6A CN201210462127A CN103814653B CN 103814653 B CN103814653 B CN 103814653B CN 201210462127 A CN201210462127 A CN 201210462127A CN 103814653 B CN103814653 B CN 103814653B
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Abstract
Seed vitality be reflection percentage of seedgermination and sprout form the quantizating index of the robustness of plant, seed vitality is higher, and to represent the value of seed larger.The present invention differentiates the vigor of tested seed by the size measuring seed oxygen gas flow rate value, can measure the vigor of an independent seed, and seed need not destructive process before measuring, and measuring process, to seed not damaged, does not affect the follow-up use of seed.The present invention effectively overcome traditional vigor measuring method can only draw statistical conclusions and single seeded vigor cannot be provided, measure after seed cannot continue the defects such as uses, simple to operate, result accurately, can be promoted the use of on a large scale.
Description
Technical field
The present invention relates to a kind of method being differentiated seed vitality by oxygen gas flow rate, be a kind of lossless detection method to seed vitality, belong to biological technical field.
Background technology
No matter life science or agricultural production, seed is all very important living resources goods and materials.The effect of seed quality to scientific research or agricultural production has tremendous influence.Seed vitality defines the generality of seed value, refer to reflect percentage of seedgermination and after sprouting form the quantizating index of the robustness of plant.According to general definition custom, seed vitality is higher, and to represent the value of seed larger.
Measuring the indexs such as germination rate is the conventional method obtaining seed vitality, but these conventional methods exist certain defect.First, their all Corpus--based Method tests, just can reach a conclusion after must adding up, and cannot learn the vigor of a certain grain specific seed to a quantity of seeds; Secondly, these indexs could be measured after all will carrying out certain destructiveness process to seed, and such as measuring germination rate must first by tested seed germination, and tested seed cannot continue after measuring to use.
The inflow and outflow of specific ion or molecule is one of essential characteristic of organism physiological function.Seed is a kind of special organism, also possesses and breathes this basic physiological function, thus the also inflow phenomenon that there is oxygen molecule the same as other biological body.Oxygen, chemical formula O
2, be that well-known biology breathes indispensable important molecule.The flow speed value of oxygen molecule inflow seed directly reflects the power of seed respiratory function, and the power of seed respiratory function is directly related with its vigor size, and therefore oxygen gas flow rate value can as differentiation seed vitality index simply, intuitively and accurately.Utilize the non-invasive micro-test technology of open report, the oxygen gas flow rate value of seed sample can be measured.
Summary of the invention
The present invention measures sample, is differentiated the vigor size of seed by the flow speed value measuring seed plumular axis position oxygen.
Measured seed sample is single seed, and the seed vitality data that measurement draws are for an independent seed, and the data utilizing statistical method to obtain after a large amount of seed sample of non-measured.
Only need tested seed quiescent culture 3-6 hour in liquid environment before measurement, other destructive pre-treatments such as need not to sprout, which ensure that measuring process does not affect the follow-up use to seed.
For realizing best measurement effect, the CaCl of 0.1mmol/L in aforesaid liquid environment, should be contained
2, 0.1mmol/L the MES of KCl and 0.3mmol/L, the pH value of liquid environment should be adjusted to 6.0.
Differentiate that the experimental design of seed vitality is by oxygen gas flow rate: tested seed is placed in liquid environment quiescent culture 3-6 hour, measure the flow velocity numerical value of seed plumular axis position oxygen afterwards, within every 10 seconds, measure data, measure 10 minutes altogether, using the mean value of the data obtained in 10 minutes as the flow speed value recorded, differentiated the vigor of tested seed by the oxygen gas flow rate value recorded.Measure the concrete operation method of lewis' acid flow velocity according to disclosed non-invasive micro-test technology document execution.According to the measuring characteristic of non-invasive micro-test technology, tested seed need not carry out the destructive process such as sprouting before measuring, and measuring process, to seed not damaged, does not affect the follow-up use of seed.
Differentiate that the concrete method of discrimination of seed vitality is by oxygen gas flow rate: when the absolute value of the oxygen gas flow rate value recorded is less than 10pmolcm
-2s
-1, differentiate that tested seed is debility seed, without value; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 10pmolcm
-2s
-1but be less than 40pmolcm
-2s
-1time, differentiate that tested seed is low vitality seed, suggestion is only for laboratory research; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 40pmolcm
-2s
-1but be less than 60pmolcm
-2s
-1time, differentiate that tested seed is medium vigor seed, small-scale land for growing field crops can also be used for plant experimentally or produce as a trial except laboratory research, but not be proposed to be used in extensive agricultural production; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 60pmolcm
-2s
-1time, differentiate that tested seed is high vigor seed, can be used for extensive agricultural production.
The method of seed vitality is differentiated by oxygen gas flow rate, directly provide single seeded vigor data, seed after measurement still can continue use, effectively overcome conventional method can only draw statistical conclusions and cannot provide the vigor of each seed, measure after seed cannot continue the defects such as use.Operation is simple for the method, result accurately and reliably, no matter for laboratory research or the needs of agricultural production can on a large scale in promote the use of.
Embodiment
With embodiment, the present invention is explained further below, but the present invention is not limited to this embodiment.
Embodiment:
Choose four kinds of soya seeds of known germination rate, measure the corresponding relation of different soya seeds oxygen gas flow rate values and its germination rate.
Usually, the seed of germination rate 90%-100% is regarded as high vigor seed, the seed of germination rate 50%-90% is regarded as medium vigor seed, and the seed of germination rate 10%-50% is regarded as low vitality seed, and the seed of germination rate 0%-10% is regarded as debility seed.The germination rate of the four kinds of soya seeds chosen is respectively 95%, 70%, 33% and 7%, represents high vigor, medium vigor, low vitality and the debility seed evaluated with germination rate.From four kinds of soya seeds, respectively randomly draw one measure its oxygen gas flow rate value.
Before measurement, the first CaCl of configuration containing 0.1mmol/L
2, 0.1mmol/L the solution of MES (being often referred to as MES in laboratory research) composition of KCl and 0.3mmol/L, the pH value of solution is adjusted to 6.0.Four tested soya seeds soak 3,4,5,6 hours respectively in the solution.
After soya seeds is dipped to the scheduled time, change the brand-new solution that composition is identical with pH value, in this solution, measure the oxygen gas flow rate value flowing into soya seeds plumular axis, measure and continue 10 minutes, within every 10 seconds, measure data.Measure after terminating 60 corresponding for every soya seeds data are average, this average is as measuring the oxygen gas flow rate value obtained.
The absolute value being the oxygen gas flow rate value that the soya seeds extracted the soybean seed representative of 95% records from germination rate is 77.38pmolcm
-2s
-1, differentiate that this seed is high vigor seed; The absolute value being the oxygen gas flow rate value that the soya seeds extracted the soybean seed representative of 70% records from germination rate is 57.02pmolcm
-2s
-1, differentiate that this seed is medium vigor seed; The absolute value being the oxygen gas flow rate value that the soya seeds extracted the soybean seed representative of 33% records from germination rate is 26.88pmolcm
-2s
-1, differentiate that this seed is low vitality seed; The absolute value being the oxygen gas flow rate value that the soya seeds extracted the soybean seed representative of 7% records from germination rate is 5.70pmolcm
-2s
-1, differentiate that this seed is debility seed.
As can be seen from above-mentioned measurement data, differentiate by germination rate, the conclusion that seed vitality draws differentiates that the conclusion that seed vitality obtains is on all four with tradition by oxygen gas flow rate, this also fully confirms that the conclusion drawn by oxygen gas flow rate differentiation seed vitality is accurately believable.
Claims (4)
1. one kind differentiates the method for seed vitality by oxygen gas flow rate, it is characterized in that, tested seed is placed in liquid environment quiescent culture 3-6 hour, measure the oxygen gas flow rate numerical value at seed plumular axis position afterwards, within every 10 seconds, measure data, measure 10 minutes altogether, using the mean value of the data obtained in 10 minutes as the flow speed value recorded, differentiated the vigor of tested seed by the oxygen gas flow rate value recorded: when the absolute value of the oxygen gas flow rate value recorded is less than 10pmolcm
-2s
-1, differentiate that tested seed is debility seed, without value; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 10pmolcm
-2s
-1but be less than 40pmolcm
-1s
-1time, differentiate that tested seed is low vitality seed, suggestion is only for laboratory research; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 40pmolcm
-2s
-1but be less than 60pmolcm
-2s
-1time, differentiate that tested seed is medium vigor seed, land for growing field crops can also be used for plant experimentally or produce as a trial except laboratory research, but not be proposed to be used in agricultural production; When the absolute value of the oxygen gas flow rate value recorded is more than or equal to 60pmolcm
-2s
-1time, differentiate that tested seed is high vigor seed, can be used for agricultural production.
2., according to the method being differentiated seed vitality by oxygen gas flow rate according to claim 1, it is characterized in that, described tested seed refers to an independent seed.
3. according to the method being differentiated seed vitality by oxygen gas flow rate according to claim 1, it is characterized in that, described seed vitality be reflection percentage of seedgermination and after sprouting form the quantizating index of the robustness of plant, seed vitality is higher, and to represent the value of seed larger.
4. according to the method being differentiated seed vitality by oxygen gas flow rate according to claim 1, it is characterized in that, the CaCl containing 0.1mmol/L in described liquid environment
2, 0.1mmol/L the MES of KCl and 0.3mmol/L, the pH value of liquid environment is 6.0.
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| CN201210462127.6A CN103814653B (en) | 2012-11-16 | 2012-11-16 | A kind of method being differentiated seed vitality by oxygen gas flow rate |
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| CN201210462127.6A CN103814653B (en) | 2012-11-16 | 2012-11-16 | A kind of method being differentiated seed vitality by oxygen gas flow rate |
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| CN103814653B true CN103814653B (en) | 2016-03-16 |
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| CN105815003B (en) * | 2016-03-23 | 2018-12-18 | 河南农业大学 | A method of seed vitality is determined based on Micro dynamic molecular flow detection technique |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101609065A (en) * | 2008-06-20 | 2009-12-23 | 旭月(北京)科技有限公司 | A kind of technology of automatic scanning micro-test of ionic/molecular activity |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101609065A (en) * | 2008-06-20 | 2009-12-23 | 旭月(北京)科技有限公司 | A kind of technology of automatic scanning micro-test of ionic/molecular activity |
Non-Patent Citations (3)
| Title |
|---|
| Influence of aging, oxygen, and moisture on ethanol production from cabbage seeds;corinne f.j. rutzke et al.;《J.Amer.soc.hort.sci》;20080131;第133卷(第1期);第158-164页 * |
| 利用Q2技术快速检测番茄种子引发后的活力;陈能阜等;《中国蔬菜》;20101015(第20期);第47-51页 * |
| 非损伤微测技术在植物活体动态离子组学研究中应用初探;许越等;《2007中国植物生理学会全国学术会议论文摘要汇编》;20070801;第263-264页 * |
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