CN103808777A - Electrochemical reaction tank for microscopic observation in dark fields - Google Patents
Electrochemical reaction tank for microscopic observation in dark fields Download PDFInfo
- Publication number
- CN103808777A CN103808777A CN201410074100.9A CN201410074100A CN103808777A CN 103808777 A CN103808777 A CN 103808777A CN 201410074100 A CN201410074100 A CN 201410074100A CN 103808777 A CN103808777 A CN 103808777A
- Authority
- CN
- China
- Prior art keywords
- electrochemical reaction
- heat insulating
- electrode
- insulating lamina
- reaction cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003487 electrochemical reaction Methods 0.000 title claims abstract description 32
- 239000011521 glass Substances 0.000 claims abstract description 11
- 238000001446 dark-field microscopy Methods 0.000 claims description 13
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 7
- 229920005573 silicon-containing polymer Polymers 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 3
- 230000005684 electric field Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 8
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 8
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 8
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 8
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000002105 nanoparticle Substances 0.000 description 6
- 238000003384 imaging method Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000013007 heat curing Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Images
Abstract
The invention relates to an electrochemical reaction tank for microscopic observation in dark fields. The electrochemical reaction tank comprises an electrochemical reaction tank body and an electrochemical original part, wherein the electrochemical reaction tank body is characterized in that an insulated thin layer I with two holes is arranged on an ITO glass sheet, and the two holes in the insulated thin layer I are connected to each other; the electrochemical original part comprises a counter electrode and a reference electrode which are respectively located and arranged in the holes in the insulated thin layer I. The electrochemical reaction tank has the advantages of simplicity in manufacture, low cost, repeated use, convenience in operation, capability of working for a long time, adjustable height and the like and is suitable for operation in dark electric fields.
Description
Technical field
The invention belongs to technical field of electrochemistry, relate to a kind of electrochemical reaction cell that can be used for dark-field microscopy.
Background technology
along with the development of modern science and technology, optical microscopy and spectral technique etc. is applied to science and technology field and production division more and more widely by updating and developing.Single-particle spectrum is a kind of traditional spectral investigation method based on micro-imaging technique.Spectrum microtechnic and TEM, SEM, STM and atomic force microscope (Atomic Force Microscope, AFM), galvanochemistry etc. are combined, become one of method the most simply and easily of research nano metallic colloid.The electrochemical reactor that can be used for using under dark field microscope of having delivered at present, exist operates inconvenience more, and reusability is poor, and device integrated complex is difficult to realize the problems such as long-time use, need to further improve.
Summary of the invention
The object of this invention is to provide a kind of electrochemical reaction cell that can be used for dark-field microscopy, solution can be used for the electrochemical reaction cell using under dark field microscope at present, and exist operates inconvenience more, and reusability is poor, device integrated complex, is difficult to realize the problems such as long-time use.
The present invention is achieved through the following technical solutions: a kind of electrochemical reaction cell that can be used for dark-field microscopy, comprise electrochemical reaction cell and galvanochemistry original paper, wherein, described electrochemical reaction cell is that heat insulating lamina with holes is installed on ito glass sheet, and the hole on heat insulating lamina one is that two and two holes are connected; Galvanochemistry original paper comprises electrode and contrast electrode, and electrode and contrast electrode are distinguished in the hole of location and installation on heat insulating lamina one.
Further, described heat insulating lamina one is dimethyl silicone polymer thin layer, hereinafter to be referred as PDMS thin layer.
Further, also comprise heat insulating lamina two, heat insulating lamina two is between heat insulating lamina one and ito glass sheet, on heat insulating lamina two, have a hole, on heat insulating lamina one, also have the 3rd hole, the 3rd hole is connected with the first two hole, and aligns with the hole on heat insulating lamina two.
Further, described heat insulating lamina two is dimethyl silicone polymer thin layer.
Further, the described bottom to electrode and contrast electrode is wound in the concentrically ringed planar structure of spiral fashion.
Further, the diameter of the described bottom helical structure to electrode and contrast electrode is slightly less than the diameter in the hole on heat insulating lamina one.
Further, also comprise electrode locating device, this device comprises base plate and sleeve pipe, and base plate longitudinal register is arranged on the side of electrochemical reaction cell and base plate and is longitudinally fixed with sleeve pipe, and sleeve pipe is set on electrode and contrast electrode.
Further, described sleeve pipe is platypelloid type.
Adopt the good effect of technique scheme: electrochemical reactor of the present invention make simple, cost is low, can Reusability, operation is convenient, the advantage such as highly adjustable that can work long hours, and is more suitable for dark electric field operation; , can well keep apart electrode and contrast electrode and working electrode meanwhile, effectively prevent short circuit.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention
Fig. 2 is the imaging of the nano particle under dark field microscope before electrochemical reaction;
Fig. 3 is the imaging of the nano particle under dark field microscope after electrochemical reducting reaction.
In figure, 1 ito glass, 2 one, 3 pair of heat insulating lamina electrodes, 4 contrast electrodes, 5 heat insulating lamina two, 6 base plates, 7 sleeve pipes.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described further, but should not be construed as limitation of the present invention:
Fig. 1 is structural representation of the present invention, as shown in the figure, a kind of electrochemical reaction cell that can be used for dark-field microscopy, comprise electrochemical reaction cell and galvanochemistry original paper, wherein, described electrochemical reaction cell is that heat insulating lamina with holes 1 is installed on ito glass sheet 1, and the hole on heat insulating lamina 1 is that two and two holes are connected; Galvanochemistry original paper comprises electrode 3 and contrast electrode 4, in electrode 3 and contrast electrode 4 holes of difference location and installation on heat insulating lamina 1.Hole on heat insulating lamina one is the space of accepting sample.Further, described heat insulating lamina 1 is dimethyl silicone polymer thin layer, can be also the materials such as other nonconducting superpolymer.Because heat insulating lamina one is very thin, therefore can realize low clearance operation under details in a play not acted out on stage, but told through dialogues.
Because distance between electrode is very little, in order to prevent short circuit, also comprise heat insulating lamina 25, heat insulating lamina 25 is between heat insulating lamina 1 and ito glass sheet 1, on heat insulating lamina 25, have a hole, on heat insulating lamina 1, also have the 3rd hole, the 3rd hole is connected with the first two hole, and aligns with the hole on heat insulating lamina 25.Increase by second thin layer, make electrode and contrast electrode and working electrode well keep apart, can well prevent short circuit.Further, described heat insulating lamina 25 is dimethyl silicone polymer thin layer, can be also the materials such as other nonconducting superpolymer.
In order to increase the working area to electrode and contrast electrode, the described bottom to electrode 3 and contrast electrode 4 is wound in the concentrically ringed planar structure of spiral fashion.Meanwhile, the diameter of the described bottom helical structure to electrode 3 and contrast electrode 4 is slightly less than the diameter in the hole on heat insulating lamina 1.
Further, also comprise electrode locating device, this device comprises base plate 6 and sleeve pipe 7, and base plate 6 longitudinal registers are arranged on the side of electrochemical reaction cell and base plate 6 and are longitudinally fixed with sleeve pipe 7, sleeve pipe 7 is set on electrode 3 and contrast electrode 4, and electrode is fixed.Described sleeve pipe 7 is platypelloid type, and Main Function is in order to prevent that electrode from rotating.
The present embodiment illustrates the making of this device.
The preparation of PDMS thin layer cover plate: Sylgard 184 monomers and hardening agent are with 10:1(mass ratio) ratio mix, degassed, on clean plane cofferdam, after heat curing, take off and can obtain with ing, adopt as required card punch punch.
Design, making PDMS(dimethyl silicone polymer) film; Cover on the ito glass by plasma treatment making a call to a PDMS solidfied material after circular hole; The PDMS thin layer with three breach holes is adhered on PDMS thin layer above, aligns with hole on the PDMS thin layer of ground floor in first breach hole of this layer.Then this reaction tank is fixed to dark field microscope below.
Glass and PDMS are placed in plasma cleaner after cleaning up respectively, processing time 2min, and bonding aligns at once.
Electrode locating device preparation: will be coiled into concentrically ringed planar structure to electrode and contrast electrode bottom helical disk, outermost circular diameter is slightly less than the bore dia of reaction tank.To the tinsel of electrode and contrast electrode be passed to a sleeve pipe, sleeve pipe is flattened, be fixed on base plate, available glue or other setting tool fixations, to prevent that in use procedure, electrode rotates.Electrode locating device is arranged on both sides the concentric collimation with detection cell.
comparative example
This device is easy to loading and unloading, reusable, easy and simple to handle.When use, with adhesive tape, the glass that connects electrode is attached on objective table, makes electrode immerse solution.Method is simple.Advantage has: 1, easy to use, can reuse; 2, between the operating period, electrode does not block and affects light path; 3, the contrast electrode of various sizes all can use; Color and the spectrum change of the nano particle before and after 4, can home position observation electrochemical reaction under dark field microscope.Fig. 2 is the imaging of the nano particle under dark field microscope before electrochemical reaction, Fig. 3 is the imaging of the nano particle under dark field microscope after electrochemical reducting reaction, as shown in Figure 2 and Figure 3, color and the spectrum change of the nano particle that this device shows are very clear, are more suitable for dark electric field operation.
Claims (8)
1. one kind can be used for the electrochemical reaction cell of dark-field microscopy, it is characterized in that: comprise electrochemical reaction cell and galvanochemistry original paper, wherein, described electrochemical reaction cell is heat insulating lamina one (2) with holes to be installed ito glass sheet (1) is upper, and the hole on heat insulating lamina one (2) is that two and two holes are connected; Galvanochemistry original paper comprises electrode (3) and contrast electrode (4), and electrode (3) and contrast electrode (4) are distinguished in the hole of location and installation on heat insulating lamina one (2).
2. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 1, is characterized in that: described heat insulating lamina one (2) is dimethyl silicone polymer thin layer.
3. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 1, it is characterized in that: also comprise heat insulating lamina two (5), heat insulating lamina two (5) is between heat insulating lamina one (2) and ito glass sheet (1), on heat insulating lamina two (5), have a hole, on heat insulating lamina one (2), also have the 3rd hole, the 3rd hole is connected with the first two hole, and aligns with the hole on heat insulating lamina two (5).
4. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 3, is characterized in that: described heat insulating lamina two (5) is dimethyl silicone polymer thin layer.
5. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 1, is characterized in that: the described bottom to electrode (3) and contrast electrode (4) is wound in the concentrically ringed planar structure of spiral fashion.
6. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 5, is characterized in that: the diameter of the described bottom helical structure to electrode (3) and contrast electrode (4) is slightly less than the diameter in the hole on heat insulating lamina one (2).
7. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 1, it is characterized in that: also comprise electrode locating device, this device comprises base plate (6) and sleeve pipe (7), base plate (6) longitudinal register is arranged on the side of electrochemical reaction cell and base plate (6) and is longitudinally fixed with sleeve pipe (7), and sleeve pipe (7) is set on electrode (3) and contrast electrode (4).
8. the electrochemical reaction cell that can be used for dark-field microscopy according to claim 7, is characterized in that: described sleeve pipe (7) is platypelloid type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410074100.9A CN103808777B (en) | 2014-03-03 | 2014-03-03 | A kind of electrochemical reaction cell that can be used for dark-field microscopy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410074100.9A CN103808777B (en) | 2014-03-03 | 2014-03-03 | A kind of electrochemical reaction cell that can be used for dark-field microscopy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103808777A true CN103808777A (en) | 2014-05-21 |
| CN103808777B CN103808777B (en) | 2016-01-20 |
Family
ID=50705860
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410074100.9A Expired - Fee Related CN103808777B (en) | 2014-03-03 | 2014-03-03 | A kind of electrochemical reaction cell that can be used for dark-field microscopy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103808777B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880453A (en) * | 2015-05-06 | 2015-09-02 | 华东理工大学 | Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949946A (en) * | 2010-09-03 | 2011-01-19 | 东华大学 | Method for producing photoelectrochemical microfluidic detection chip of three-electrode system |
| CN102183669A (en) * | 2011-02-15 | 2011-09-14 | 中国科学院化学研究所 | Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof |
| CN202421123U (en) * | 2012-01-10 | 2012-09-05 | 刘钧 | Electrode device for analyzing micro-potential dissolution |
| CN102654477A (en) * | 2012-04-23 | 2012-09-05 | 华东理工大学 | Dark-field nano spectral electrochemical detection pool for plasma observation |
| CN102749322A (en) * | 2012-07-04 | 2012-10-24 | 浙江大学 | Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array |
-
2014
- 2014-03-03 CN CN201410074100.9A patent/CN103808777B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949946A (en) * | 2010-09-03 | 2011-01-19 | 东华大学 | Method for producing photoelectrochemical microfluidic detection chip of three-electrode system |
| CN102183669A (en) * | 2011-02-15 | 2011-09-14 | 中国科学院化学研究所 | Micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and preparation method thereof |
| CN202421123U (en) * | 2012-01-10 | 2012-09-05 | 刘钧 | Electrode device for analyzing micro-potential dissolution |
| CN102654477A (en) * | 2012-04-23 | 2012-09-05 | 华东理工大学 | Dark-field nano spectral electrochemical detection pool for plasma observation |
| CN102749322A (en) * | 2012-07-04 | 2012-10-24 | 浙江大学 | Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880453A (en) * | 2015-05-06 | 2015-09-02 | 华东理工大学 | Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103808777B (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Aria et al. | Time evolution of the wettability of supported graphene under ambient air exposure | |
| Tzouvadaki et al. | Label-free ultrasensitive memristive aptasensor | |
| Yuan et al. | Engineering ultra-low work function of graphene | |
| Sun et al. | A multiscale TiO2 nanorod array for ultrasensitive capture of circulating tumor cells | |
| CN103199020B (en) | Based on preparation method and the detection method of the liquid grid-type graphene field effect pipe of PI | |
| Cho et al. | High-sensitivity and low-power flexible schottky hydrogen sensor based on silicon nanomembrane | |
| Kim et al. | Poly (ethylene glycol)-modified tapered-slit membrane filter for efficient release of captured viable circulating tumor cells | |
| Liu et al. | Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood | |
| Tabassian et al. | Graphene mesh for self‐sensing ionic soft actuator inspired from mechanoreceptors in human body | |
| Santhanagopalan et al. | High-voltage electrophoretic deposition for vertically aligned forests of one-dimensional nanoparticles | |
| CN105021680A (en) | Graphene sensor based method for detecting MicroRNA | |
| CN103861469A (en) | Method for preparing tubular graphene composite membrane | |
| CN105772118A (en) | SERS (Surface Enhanced Raman Spectroscopy) microfluidic chip with enhanced substrate integrated on ITO conductive glass and preparation method for SERS microfluidic chip | |
| CN102071135A (en) | High resolution patch clamp based on scanning probe microscopy technology and operating method thereof | |
| Martinez et al. | SU-8 hollow cantilevers for AFM cell adhesion studies | |
| BR112014007672A2 (en) | method for collecting cellular material and installing for performance of said method | |
| Zhu et al. | A gold nanoparticle-modified indium tin oxide microelectrode for in-channel amperometric detection in dual-channel microchip electrophoresis | |
| Jeon et al. | Hydrophobic surface treatment and interrupted atomic layer deposition for highly resistive Al2O3 films on graphene | |
| CN103808777B (en) | A kind of electrochemical reaction cell that can be used for dark-field microscopy | |
| Bae et al. | Stretchable non‐enzymatic fuel cell‐based sensor patch integrated with thread‐embedded microfluidics for self‐powered wearable glucose monitoring | |
| Huang et al. | Ultrathin self-assembled diphenylalanine nanosheets through a gold-stabilized strategy for high-efficiency adsorption/desorption/ionization | |
| Bagheri et al. | Electrospun titania sol–gel‐based ceramic composite nanofibers for online micro‐solid‐phase extraction with high‐performance liquid chromatography | |
| CN101452020A (en) | In-situ measurement material surface resistivity method under vacuum environment | |
| Yanagi et al. | Sub-10-nm-thick SiN nanopore membranes fabricated using the SiO2 sacrificial layer process | |
| Arya et al. | Substrate Versatile Roller Ball Pen Writing of Nanoporous MoS2 for Energy Storage Devices |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 Termination date: 20180303 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |